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Steroid saponin compound in siberian fritillary bulb

A technology of irbe saponins and steroidal saponins, which is applied in the field of steroidal saponins, and can solve the problem of not finding non-alkaloid components

Active Publication Date: 2012-06-20
深圳弘汇生物医药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, there are still many problems in the chemical research on the non-alkaloid components of Fritillaria, and no non-alkaloid components have been found to exert antihypertensive, anticholinergic, anti-inflammatory, anti-tumor, antispasmodic, antiasthmatic, antitussive, and expectorant properties. and antibacterial active ingredients

Method used

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  • Steroid saponin compound in siberian fritillary bulb
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  • Steroid saponin compound in siberian fritillary bulb

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Example 1: Isolation of Compounds

[0077] With 2 kg of dried bulbs of Igami (Fritillaria Pallidiflora Schrenk.), the ethanol with a volume fraction of 95% was extracted three times for 3 hours each time, and the solvent consumption was 10, 10, 8, and the combined filtrate was decompressed After recovering the solvent, 89.0 g of extract was obtained. The medicinal dregs extracted with 95% ethanol were extracted three times with 70% ethanol by volume fraction for 3 hours each time, the solvent consumption was 10, 10, 8, the filtrate was combined, and the solvent was recovered under reduced pressure to obtain 98.0 g of extractum. Add appropriate amount of 2L of water to disperse the obtained extract, and extract with equal volumes of petroleum ether, chloroform, ethyl acetate, and n-butanol in sequence (each solvent is extracted three times). After recovering the solvent, 2.2 g of petroleum ether layer extract, 3.5 g of chloroform layer, 5.1 g of ethyl acetate layer, and...

Embodiment 2

[0082] Embodiment 2: identification of chemical structure

[0083] The chemical structures of the nine isolated compounds were determined by 1-dimensional and 2-dimensional nuclear magnetic resonance (1D, 2D-NMR), mass spectrometry (MS), circular dichroism (CD) and other physical and chemical methods. Its chemical structure and identification means are shown in Table 1:

Embodiment 3

[0084] Embodiment 3: HPLC-TOF-MS analysis

[0085] Configuration of the reference solution: Ibeta saponin A, Ibeta saponin B, Ibeta saponin C, Ibeta saponin D, Ibeta saponin E, Ibeta saponin F, Ibeta saponin G, Ibeta saponin H, Ibeta saponin I Precisely weigh an appropriate amount of self-made chemical reference substance, and prepare a 5 μg / ml reference substance solution with methanol.

[0086] Configuration of sample solution: Precisely weigh 5g of each Fritillaria sample, extract with 100ml chloroform Sabouraud extractor for 3 hours, recover chloroform extract, dissolve in 10ml methanol, pretreat with SPE small chromatographic column filled with ODS, 95% acetonitrile Wash with 20ml, combine methanol and 95% acetonitrile eluent, dissolve in methanol, dilute to 5ml volumetric flask, filter with 0.2μm filter membrane, and obtain.

[0087] Test method: Inject 10 μl of sample, perform HPLC-TOF-MS analysis, use the M+H peak of each compound as the selected ion to obtain the chr...

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Abstract

The invention relates to a steroid saponin compound in siberian fritillary bulb extracted from a Chinese medicine, i.e., Siberian fritillary bulb, a preparation process thereof and medicinal uses of asthma preventing, cough relieving, phlegm eliminating and the like thereof. A composition can be applied to medicaments, functional foods and foods. The compound is extracted from fritillary plants based on medicinal materials including thunberg fritillary bulb, unibract fritillary bulb, siberian fritillary bulb, hupeh fritillary bulb, ussuriensis fritillary bulb or fritillaria anhuiensis.

Description

Technical field: [0001] The present invention relates to the active ingredient isolated from Ignebra, especially the antihypertensive, anti-cholinergic, anti-inflammatory, anti-tumor, antispasmodic, anti-asthma, antitussive, expectorant and antibacterial effects New steroidal saponins. Background technique: [0002] Fritillaria is a kind of Fritillaria of Liliaceae, including a variety of important medicinal plants, and its medicinal parts are mostly underground bulbs. Fritillaria has the effects of clearing away heat and moistening lungs, relieving cough and eliminating phlegm. According to records, there are about 130 species of this genus. kind. In my country, its types mainly include Chuan Fritillaria, Zhejiang Fritillaria, and Yi Fritillaria. Modern research on the chemical components of Fritillaria mainly focuses on its alkaloid components. The alkaloid components in Fritillaria mainly include steroidal alkaloids and isosteroid alkaloids. Antitussive, antispasmodic,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J71/00C07J17/00A61K31/7048A61K31/704A61P11/06A61P11/14A61P11/10A61P31/04A61P9/10A61P9/00A61P25/00A61P29/00A61P37/08A61P11/00A61P1/16A61P35/00A61P3/06A61P3/10A61P37/04A61P39/06A23L1/30G01N30/02
Inventor 李国玉沈硕
Owner 深圳弘汇生物医药有限公司
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