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Anemarrhena asphodeloides bge steroid saponin compound, and preparation method and application thereof

A technology for steroidal saponins and compounds, applied in the field of medicine, can solve problems such as patents or literature reports that have not yet been found, and achieve the effects of significant anti-tumor activity, simple extraction and separation methods, and novel structures

Active Publication Date: 2019-12-31
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The steroidal saponin compound involved in the present invention and its activity have not been found to have patents or literature reports so far

Method used

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  • Anemarrhena asphodeloides bge steroid saponin compound, and preparation method and application thereof
  • Anemarrhena asphodeloides bge steroid saponin compound, and preparation method and application thereof
  • Anemarrhena asphodeloides bge steroid saponin compound, and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0035] Preparation of steroidal saponins

[0036] Weigh 10kg of dried rhizomes of Anemarrhena vulgaris and place them in an extraction tank. Add 50L of 75% ethanol. After soaking overnight, heat in a water bath to reflux and extract for 3 hours. Filter, and extract the filter residue twice with 75% ethanol (50L), each for 3 hours, and combine three times The filtrate was concentrated to obtain an extract-like ethanol extract (3.09kg). The ethanol extract is suspended in purified water to obtain a suspension, and then extracted with an equal volume of petroleum ether, ethyl acetate, and water-saturated n-butanol three times in sequence, and the extract is concentrated to obtain an extract of three parts. The n-butanol extract (600g) was roughly divided by macroporous adsorption resin, and the first gradient elution was carried out with 10%, 30%, 50%, 70%, 95% (v / v) ethanol-water. The eluate from the 70% ethanol fraction was crudely separated by silica gel column chromatography. ...

Embodiment 2

[0050] Anti-tumor activity test of compound I

[0051] 1. Cell lines and reagent materials Human breast cancer cell line MCF-7 and human liver cancer cell line HepG2 were provided by the American Type Culture Collection (ATCC); DMEM medium, RPMI-1640 medium, fetal bovine serum, trypsin, DMSO And MTT etc.

[0052] 2. MTT method to detect cell survival rate

[0053] Culture the MCF-7 and HepG2 cell lines with RPMI-1640 medium containing 10% fetal bovine serum, and take the MCF-7 and HepG2 cell lines that are in the logarithmic growth phase and are in good condition. The medium is discarded and pre-cooled aseptically Wash three times with PBS, then digest with trypsin solution and observe with an inverted biological microscope. After digestion, the cells are mixed evenly. Spread the MCF-7 and HepG2 cell lines on a 96-well plate respectively to ensure that the MCF-7 cell line is 2500 cells / well and the HepG2 cell line is 2000 cells / well. The drug is administered at constant temperature...

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Abstract

The invention discloses an anemarrhena asphodeloides bge steroid saponin compound, and a preparation method and an application thereof in preparation of an antitumor drug. The compound is spirostanolsteroid saponin, the molecular formula of the compound is C45H74O17, and the molecular weight of the compound is 886. The compound is prepared by extracting medicinal plant anemarrhena asphodeloides bge with 75% ethanol, extracting with water-saturated n-butyl alcohol, and separating and purifying by macroporous adsorption resin, silica gel column chromatography, ODS reversed-phase column chromatography, gel column chromatography and other methods. MTT experimental results show that the compound has remarkable anti-tumor activity on human breast cancer cell strains (MCF-7) and human liver cancer cell strains (HepG2), and is expected to become an anti-tumor candidate drug.

Description

Technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to an Zhimu steroidal saponin compound, a preparation method thereof, and application in the preparation of antineoplastic drugs. Background technique [0002] As the main cause of human death, cancer has become a global public health problem. Because cancer has the characteristics of high mortality, low treatment efficiency, and difficult to control, many scientists are committed to developing new treatment methods and new chemotherapy strategies for the treatment of various cancers. However, because some treatment programs have obvious side effects and high treatment costs, in recent years, countries have turned their attention to natural medicines. [0003] Natural medicines have gradually become the focus of cancer treatment drug research due to their advantages of wide targets and low toxicity, and great progress has been made in cancer treatment. At present, many drugs...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J71/00A61P35/00A61K31/7048
CPCC07J71/0005A61P35/00
Inventor 黄雪峰张敏杰赵云芳
Owner CHINA PHARM UNIV
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