58results about How to "Simple extraction and separation method" patented technology

The invention discloses a total bufogenin extract with antitumor function and also discloses a preparation method of the extract and application thereof in the preparation of antitumor medicines. The total bufogenin extract of the invention is prepared by organic solvent extraction, column chromatography separation and purification of dried toadskin; the content of total bufogenin is over 80 percent, wherein the content of resibufogenin is over 12 percent, the content of cinobufagin is over 4 percent, and the content of bufotalin is over 10 percent. Compared with the prior art, the invention eliminates bufotenine alkaloid which has irritation, is easy to cause hypersensitivity but has no antitumor activity, efficiently collects the effective part of total bufogenin which has significant antitumor activity, therefore pharmacological action is stronger, the clinical curative effect is higher and the toxic and side effect is lower. The total bufogenin extract can be prepared into various pharmaceutical preparations for clinical application, therefore the invention has the functions of killing tumor cells, inducing differentiation and apoptosis of tumor cells, inhibiting the neovascularization of tumor cells and lowering the neoplasm metastasis rate, etc.

The invention relates to a method for extracting a euscaphis konishii hayata high-efficiency anti-inflammatory action compound and a product. The method mainly comprises the following steps: taking a euscaphis konishii hayata plant, chopping, drying at the temperature of 50 DEG C and grinding; taking the plant powder, condensing and performing reflux extraction for three times by using distilled water, wherein the extraction time is 3 hours each time; filtering and merging so as to obtain the filtrate, extracting the filtrate by using n-butyl alcohol, extracting for three times, merging the n-butyl alcohol extracting solution, performing rotary evaporation and concentration, thereby obtaining the extract S, namely, the product provided by the invention. The extraction and separation method is simple and rich in resources, the extract is obvious in effects of diminishing inflammation and inhibiting capillary permeability, and the effects of the extract are obviously higher than those of dexamethasone injection.

The invention discloses an anemarrhena asphodeloides bge steroid saponin compound, and a preparation method and an application thereof in preparation of an antitumor drug. The compound is spirostanolsteroid saponin, the molecular formula of the compound is C45H74O17, and the molecular weight of the compound is 886. The compound is prepared by extracting medicinal plant anemarrhena asphodeloides bge with 75% ethanol, extracting with water-saturated n-butyl alcohol, and separating and purifying by macroporous adsorption resin, silica gel column chromatography, ODS reversed-phase column chromatography, gel column chromatography and other methods. MTT experimental results show that the compound has remarkable anti-tumor activity on human breast cancer cell strains (MCF-7) and human liver cancer cell strains (HepG2), and is expected to become an anti-tumor candidate drug.

The invention provides eucommia ulmoides extract and an application thereof in treatment of osteoporosis, and belongs to the technical field of traditional Chinese medicine extraction. The eucommia ulmoides extract comprises the following components in parts by weight: 20-50 parts of eucommia ulmoides petroleum ether extract and 10-30 parts of eucommia ulmoides ethanol extract; the eucommia ulmoides extract can be effectively used for treating the osteoporosis by controlling the weight part ratio of the eucommia ulmoides petroleum ether extract to the eucommia ulmoides ethanol extract to be (1-5): 1. The invention further provides an extraction method of the eucommia ulmoides petroleum ether extract and the eucommia ulmoides ethanol extract. Due to the fact that different effective components are different in solubility in different solvents, the content of the effective components can be effectively adjusted by adopting different solvents for extraction; the used extraction separationmethod is simple and easy to implement; and the osteoporosis treating effect is good.

The invention relates to the technical field of food and traditional Chinese medicines and provides a preparation method and applications of three novel compounds extracted and separated from paeoniaanomala. Chemical structural formulas of the compounds are shown in formulas (I), (II) and (III). Dried root tubers of paeonia anomala are crushed and then subjected to heating reflux extraction with80% ethanol, a total extract obtained through reduced pressure concentration is dispersed with water, extraction is performed with petroleum ether, ethyl acetate and water-saturated n-butyl alcohol with the same volume respectively, and parts are obtained. The n-butyl alcohol part is dissolved with water, macroporous resin column chromatography is performed, and fractions are obtained; the 30% ethanol part is separated and purified through silica gel column chromatography, a target chromatographic liquid is collected, separation with a C18 reversed phase chromatographic column and purificationwith Sephadex LH-20 (hydroxypropyl sephadex) are performed, and the compounds I, II and III are obtained. The invention also provides applications of the three compounds in neuroprotection and neurodegenerative disease treatment.

The invention discloses an application of a cardiac glycoside compound 12 beta-hydroxycalotropin in preparing medicines for treating and preventing tumors. The cardiac glycoside compound 12 beta-hydroxycalotropin has obvious antitumor activity and can be served as a lead compound for treating the tumors. Moreover, an extraction and separation method of the cardiac glycoside compound 12 beta-hydroxycalotropin provided by the invention is simple and practicable and low in cost, and has high efficiency and yield and good extract quality.

The invention relates to new application of a hydroquinone compound, particularly application of a hydroquinone compound (shikonin) in preparing medicines for resisting hepatitis C virus (HCV). The hydroquinone compound (shikonin) has obvious activity for resisting HCV, and the selection index is up to 43.56; and thus, the hydroquinone compound (shikonin) can be used as a lead compound for treating hepatitis C. The molecular formula of the hydroquinone compound (shikonin) is C16H16O5, and the chemical structural formula is disclosed in the specification.

The invention provides a novel 3,4-ring-opening polyacylation grayananes diterpene compound and also relates to a method for extracting and separating the 3,4-ring-opening polyacylation grayananes diterpene compound from Pieris Formosa. The obtained compound is determined as 3,4-seco-3,10-olide-5beta-hydroxy-6beta,7alpha, 11alpha,15alpha,16alpha-pentaacetoxy-14beta-propionyloxygrayan-4(19)-ene through structural identification and is called as pieris formosin E(1), and 3,4-ring-opening polyacylation grayananes diterpene which is discovered for the first time has the structural formula.

The invention belongs to the field of medicinal compounds, and concretely discloses marine fungus-derived depside compounds and an application thereof in treatment of type 2 diabetes. The structure of the depside compounds is represented by formula (I). The compounds 1-6 can substantially inhibit the activity of alpha-glucosidase, the IC50 value of the compound 1 is 10.4[mu]M, the IC50 value of the compound 2 is 13.3[mu]M, the IC50 value of the compound 3 is 2.1[mu]M, the IC50 value of the compound 4 is 12.4[mu]M, the IC50 value of the compound 5 is 9.8[mu]M, and the IC50 value of the compound 6 is 11.7[mu]M, wherein the IC50 value of positive control acarbose is 553.7[mu]M. Kinetic researches of the alpha-glucosidaseinhibition effect show that the compounds 1, 3 and 5 are noncompetitive inhibitors. The compounds can be used for preparing alpha-glucosidase inhibitor medicines in order to prevent and treat the type 2 diabetes.

The invention discloses the use of cardiac glycoside compound 12 beta-hydroxycalotropin in preparing medicines having tumor treating and preventing effects. The cardiac glycoside compound 12 beta-hydroxycalotropin has remarkable anti-tumor activity and is capable of serving as a lead compound for treating tumors; meanwhile, a method for extracting and separating the cardiac glycoside compound 12 beta-hydroxycalotropin provided by the invention is simple, feasible, low in cost, and high in efficiency and yield; the extract is good in quality.

The invention discloses a preparation method of separating lysozyme by amino-functionalized magnetic nanoparticles and specifically designs a method for separating lysozyme from egg white by the use of a magnetic nanoparticles adsorption material. According to the preparation method, Fe3O4 nanoparticles are selected and undergo surface-amino functionization modification so as to obtain amino-functionalized magnetic nanoparticles; the nanoparticles are added into an egg white solution and are saturated after 1 h of adsorption at 37 DEG C; the amino-functionalized magnetic nanoparticles saturated by adsorption are added into a phosphate buffer (20 nmol, pH=8.0, 1 mol/L NaCl) to be eluted at 37 DEG C for 2 h; and magnetic separation is carried out to obtain a solution, the eluate is put into a refrigerator of 4 DEG C, and centrifugation is carried out to obtain a solid enzyme powder. The separation method for extraction of egg white lysozyme is simple and has high separation efficiency. The obtained lysozyme has high purity. In addition, conditions are mild and have little influence on lysozyme activity, and recovery rate is high.

The invention discloses a method for purifying quercetin glucoside in semen lepidii. The method is characterized by comprising the following steps of (1) carrying out primary water extraction on quercetin-3-O-beta-D-glucose-7-O-beta-D-gentiobioside in the semen lepidii; (2) performing alcohol precipitation and impurity removal on the semen lepidii water extract; (3) eluting and separating by using LX-68G type macroporous resin; (4) eluting and purifying with a CM-agarose gel FF column; and (5) carrying out preparative liquid phase purification, so as to obtain a quercetin-3-O-beta-D-glucose-7-O-beta-D-gentiobioside monomeric compound. Experimental results show that the CM-agarose gel FF has a good purification effect on quercetin-3-O-beta-D-glucose-7-O-beta-D-gentiobioside, the purity of a sample subjected to preparative liquid phase separation and purification can reach 99.0% or above, the technical requirement that the purity of the sample is larger than 98.5% of a national traditional Chinese medicine reference substance is met, and the obtained product can be used as the traditional Chinese medicine reference substance.

The invention discloses an aryl dihydronaphthalene lignan compound with antineoplastic activity and a preparation method and application thereof, the aryl dihydronaphthalene lignan compound has the following structural general formula and belongs to the technical field of medicine, and the compound is extracted and separated from dry rhizome of rhizoma drynariae. Meanwhile, the compound 1 is prepared from 12-hydroxylauric acid through methylation reaction, esterification reaction and oxidative coupling reaction; the compound 2 is prepared by carrying out oxidative coupling reaction on a known compound glycyl 12-caffeoyloxane obtained by separation, and the compound 2 is prepared by carrying out oxidative coupling reaction on the known compound glycyl 12-caffeoyloxane; the compound disclosed by the invention can be obtained through extraction separation and can also be obtained through chemical synthesis; the compounds 1 and 2 disclosed by the invention have very strong selective inhibition activity on a human cervical cancer HeLa cell strain.

The invention specifically relates to a novel flavonoid compound extracted and separated from Bupleurum marginatum and a preparation method thereof, belonging to the technical field of medicine. The compound has a chemical structure formula as shown in a formula I which is described in the specification. The preparation method for the flavonoid compound extracted and separated from Bupleurum marginatum comprises the following successive steps: extracting; concentration; extraction; silica gel column chromatographic separation; and Sephadex LH-20 chromatographic separation. The invention has the advantage that the extraction and separation method for the novel flavonoid compound extracted from Bupleurum marginatum is simple and has good reproducibility.