Polycyclic polyisopentenyl acyl phloroglucinol compound and its preparation method and application
A technology for polycyclic polyprenyl acyl phloroglucinol and prenyl acyl phloroglucinol, applied in the field of medicine, can solve problems such as toxic and side effects, and achieve the effects of simple extraction and separation methods and easy raw materials
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Embodiment 1
[0045] Preparation and structural identification of polycyclic polyisopentenyl acylphloroglucinol compounds involved in the present invention
[0046] 1. Preparation of compound:
[0047] Step 1: Mince the whole plant of the material A. spp. (12.9 kg), and extract it by cold soaking with ethanol-water solution with a volume fraction of 60% to 99% for 2 to 4 times, each time for 12 to 24 hours. Preferably, the volume fraction is 70% to 95% ethanol-water solution for cold soaking and extraction for 2 to 3 times, each time for 18 to 24 hours. The soaking liquid was recovered and concentrated under reduced pressure to obtain 949.68 g of crude ethanol extract, which was extracted with an equal volume of petroleum ether and then concentrated under reduced pressure to obtain crude extract.
[0048] Step 2: Pass 150.7 g of the petroleum ether crude extract in step 1 through silica gel column chromatography, using cyclohexane and ethyl acetate as mobile phases (1:0, 50:1, 25:1, 10:1, ...
Embodiment 2
[0066] Example 2: Anti-breast cancer and cervical cancer effects of the compounds involved in the present invention targeting the nuclear receptor RXRα
[0067] 1 Materials and Instruments
[0068] 1.1 Experimental cells: human breast cancer MCF-7 and cervical cancer HeLa cells
[0069] 1.2 Experimental Instruments
[0070] Inverted Microscope Japan Olympus Leica Company
[0071] Cell incubator American Thermo company
[0072] Millipore Ultrapure Water Meter Millipore, USA
[0073] High-speed refrigerated centrifuge American beckMan Coulter Co., Ltd.
[0074] Micropipette Eppendorf, USA
[0075] Protein vertical electrophoresis instrument Bio-Rad, USA
[0076] Wet transfer tank American Bio-Rad company
[0077] Disposable petri dish Belgium Orange Scientific company
[0078] 48-well culture plate, Orange Scientific, Belgium
[0079] 96-well culture plate, Orange Scientific, Belgium
[0080] Vertical Automatic Electric Pressure Steam Sterilizer Shanghai Shenan Medical...
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