29 results about "Inverted microscope" patented technology

The application discloses a sectioning method and imaging method for high-resolution imaging of intact thick biological tissues, and the sectioning method comprises the following steps: S1, placing a cover glass on a first object slide and covering both with adhesive tape; S2, digging off the adhesive tape covered in the area of the cover glass, and processing the edges of the dug-off area with a hydrophobic medium; S3, transferring a tissue sample to be observed to the cover glass, absorbing the sample liquid, adding an anti-fluorescence quencher dropwise, absorbing the sample solvent after standing, adding gel dropwise, and fixing the sample on the cover glass; S4, removing the cover glass and placing it on a second object slide, so that the tissue sample is located between the cover glass and the second object slide, and the cover glass is fixedly connected with the second object slide through the adhesive tape, and the sectioning is completed. The application can observe the distribution and quantity of specific cells in thick tissues at high resolution while keeping the integrity of the tissues, and the preparation method is simple, is not limited by light paths, sizes and the like, and is suitable for most upright or inverted microscopes.

The embodiment of the invention discloses a visual nanoneedle transfection micro-fluidic chip, a preparation method and a cell transfection method.The micro-fluidic chip comprises a patterned glass nanoneedle substrate and a transparent PDMS micro-channel layer, the transparent PDMS micro-channel layer is bonded to the patterned glass nanoneedle substrate, and the transparent PDMS micro-channel layer is bonded to the patterned glass nanoneedle substrate. The patterned glass nanoneedle substrate and the transparent PDMS micro-channel layer jointly form a light-transmitting structure and are compatible with a fluorescent inverted microscope; wherein the surface of the patterned glass nanoneedle substrate is provided with a nanoneedle array of which the pinpoint height range is 700-1000nm and the pinpoint diameter range is 50-200nm; the transparent PDMS micro-channel is a micro-channel prepared from a high-transparency PDMS material, and the micro-channel comprises a channel main body, a channel inlet formed in one end of the channel main body and a channel outlet formed in the other end of the channel main body; wherein the height range of a flow channel in the flow channel main body is 10-25 microns, the width range of the flow channel is 10-100 microns, and a flow channel inlet is used for being connected with an external micro-flow pump.

This invention relates to the field of microscopy and discloses an inverted microscope, including a base with an eyepiece for human observation, an objective lens for magnifying objects rotatably connected to the base, an illumination lamp for increasing brightness, and a transparent stage for placing samples, positioned directly above the objective lens and below the illumination lamp, all fixedly connected to the base via a bracket. The base also includes a cleaning assembly, a dust removal assembly, and a sweeping assembly. This invention effectively avoids the problem of difficulty in removing dust and impurities remaining on the stage, which can affect the stage's light transmittance. Furthermore, the cleaning process on the stage surface also removes dust from the objective lens, improving the device's practicality.

The invention provides a CO2-water / salt water displacement interface instability characterization system and method based on a microfluidic technology, and belongs to the field of carbon dioxide salt water layer sealing. According to the invention, a micro-fluidic chip with specific pore structure characteristics is designed, a micro-fluidic visual experiment system based on an inverted microscope is established, and characterization parameters including capillary number, viscosity ratio, fractal dimension and the like are extracted by carrying out digital processing on continuously collected image sequences; the interface evolution characteristics of CO2 displacement water / salt water are described through the capillary number and the viscosity ratio, and the interface complexity is quantitatively described by means of fractal dimensions so as to reflect the instability of the interface. According to the method, the interface instability in the displacement process is converted into a numerical value capable of being accurately predicted from traditional qualitative description, the influence mechanism of the pore structure, the viscosity ratio, the capillary tube number and the like on the interface instability can be visually revealed, and key data support is provided for CO2 geological sequestration evaluation.

The invention discloses a low-density sperm braking method without a PVP medium in ICSI fertilization, and relates to the field of assisted reproduction therapy, and the method comprises the following steps: step 1, diluting a sperm suspension until the density is 0.1 * 10 < 6 > / ml, and placing the diluted sperm suspension in an in-vitro operating solution without PVP; step 2, under an inverted microscope, directly pressing and braking the tail of the sperm in the in-vitro operating fluid by using a microinjection needle, so that the tail of the sperm is folded or curled to deform; and step 3, transferring the braked sperms into operation liquid drops containing ovum to complete microinjection, and performing tail pressing operation in an isotonic operation liquid without PVP (Polyvinyl Pyrrolidone), so that the tail parts of the sperms present visible curled deformation or tail folding. The sperm motility destruction rate is 100% (residual motility is completely prevented from interfering fertilization), and the tail cell membrane rupture rate is 100% (it is ensured that activation factors in sperms are fully released).

Femtosecond pulse near-infrared Gaussian laser is adopted as a unified light source and is simultaneously used for time-sharing scanning optical tweezers capturing and two-photon fluorescence excitation, and a light path part is sequentially provided with a height adjusting assembly, a galvanometer scanning element, a beam expanding assembly and a telescope assembly. The light is reflected by a dichroscope configured on an inverted microscope platform, strong focusing is realized in a PDMS sample chamber by a high numerical aperture objective lens, bright field illumination is provided by a white light LED lamp, and a two-photon fluorescence signal is collected by a CMOS camera; a high-precision biosensing system based on a CRISPR / Cas12a system is constructed on the surface of a microsphere enriched with a detection signal output unit, quenching-recovery conversion of a fluorescence signal is realized, and two-photon fluorescence imaging is carried out in a multiple optical capture microsphere array formed by time-sharing scanning optical tweezers. The method has the advantages of high detection accuracy and sensitivity, low background signal, good imaging stability, strong anti-interference capability in a complex biological medium and the like.

The utility model relates to observation device technical field belongs to a kind of mouse live imaging fixing device based on inverted microscope, including fixed on the fixed device box base body and base of microscope platform, the fixed device box base body bottom is fixedly connected on base, the upper portion of fixed device box base body is equipped with detachable seal cover, the base is opened on the observation window, the fixed device box base body perimeter is also opened experimental fixed hole. The utility model can flexibly match the tissue of surgical exposure mouse and the mouse of surgical installation observation window are observed using inverted microscope, reduce the development cost of multiple sets of fixed device, can also achieve certain mouse physiological state maintenance function, and compatible various equipment required by experiment, simplify live imaging observation procedure.

This invention discloses an illumination device for solving the concave liquid surface effect in well plate imaging, belonging to the field of microscope light source technology. The device includes an inverted microscope illumination source and several annular light sources distributed in a dome-like pattern around it. The inverted microscope illumination source is composed of a central light source, a collimating lens, and a condenser lens in sequence. The annular light sources contain multiple LED beads, and the size of the rings matches the diameter of the microwells in a common cell culture plate, with adjustable angles. Through the synergistic effect of traditional vertical illumination and dome-shaped oblique illumination, this invention effectively improves the imaging quality of well plates, adapts to microwell plates of different diameters, is easy to operate, and highly practical, providing strong support for cell observation and other related work.

The utility model belongs to the technical field of scanning ionic conductance microscopes, and discloses a scanning ionic conductance microscope platform for single cell scanning and detection, which comprises an inverted microscope body, a culture dish tray of the inverted microscope body is detachably connected with a protection device, and the protection device is internally provided with an accommodating cavity for embedding a culture dish. The containing cavity extends to the bottom of the protection device, a first observation hole communicated with the top of the containing cavity is formed in the top of the protection device, a transparent cover plate is arranged on the upper side of the first observation hole, and a second observation hole is formed in the transparent cover plate; a carbon dioxide concentration detection device is arranged in the containing cavity, a carbon dioxide input hole and a carbon dioxide concentration detection device wire passing hole are further formed in the protection device, and the protection device is connected with a carbon dioxide supply device through the carbon dioxide input hole. According to the scanning ion conductance microscope platform, on the basis of an existing scanning ion conductance microscope platform, the gas environment of cells during in-vitro culture can be guaranteed, and meanwhile target cells can be conveniently found.

This invention relates to the field of fecal testing, and more specifically, to a method for detecting human parasitic pathogens without the use of slides and coverslips, comprising the following steps: Step 1, adding an appropriate amount of water to the culture wells of a cell culture plate, placing an appropriate amount of feces in the water of the culture well and stirring evenly; Step 2, cutting a piece of textile or nylon mesh of similar size to the culture well and placing it into the culture well to isolate the fecal residue and fecal liquid from each other; Step 3, after stirring and mixing the fecal liquid, transferring the fecal liquid to the culture wells of a new cell culture plate, covering it with the cell plate cover, and examining it under a microscope using an inverted microscope.

The utility model relates to the technical field of cell culture consumables, in particular to a dynamic stretching cell culture dish capable of real-time microscope observation and marker detection, which comprises a culture dish main body, a bottom membrane and a sensor, the bottom film covers the bottom surface of the culture dish main body; the culture dish main body can be stretched along a set direction, and the upper surface of the culture dish main body is provided with a culture pond penetrating through the upper surface and the lower surface; among the side walls around the culture pond, the side wall parallel to the stretching direction is a stretching side wall, and the side wall perpendicular to the stretching direction is a fixed side wall; two groups of clamping plates are symmetrically arranged in the culture pond, each group of clamping plates is arranged close to the fixed side wall, and a set distance is formed between each group of clamping plates and the fixed side wall; and the sensors are arranged between each group of clamping plates and the fixed side wall. Stretching along a set direction to implement mechanical stimulation on the cells; the bottom membrane with limited thickness is matched with the inverted microscope, so that the change condition of the cells after mechanical stimulation can be observed; the sensor can detect cell secretion factors in real time.

The utility model discloses an inverted microscope multicolor fluorescence illumination device, including microscope body, the front end of microscope body is provided with the focusing knob, the top left side of microscope body is connected with the observation cylinder, the top of observation cylinder is installed with the ocular lens, the top middle section of microscope body is equipped with the accommodation groove, the upper end of accommodation groove is connected with the object table, the inner chamber bottom of accommodation groove is installed with the objective lens, the lower extreme of accommodation groove is provided with the fluorescence filter component, the top right side of microscope body is connected with the stand, the cross end of stand is connected with the light collector, the right end surface bottom of microscope body is connected with T type rail, the outside of T type rail is equipped with the supporting plate, the top of supporting plate is connected with the illumination box, the convenience of multicolor fluorescence observation is greatly promoted, is favorable for improving the imaging quality of fluorescence image to obtain clearer, reliable observation result.

The invention discloses a multichannel fluorescence imaging system based on time sequence pulse excitation and asynchronous acquisition, and particularly relates to the technical field of biomedical engineering and optical microscopic imaging. Comprising an inverted microscope main body, a multi-wavelength laser excitation module, a high-speed Scmos imaging device supporting hardware triggering, a lower computer time sequence control unit based on a microcontroller, and an upper computer calculation processing platform. The lower computer utilizes a hardware timer to generate a synchronous control signal with microsecond-level precision, adopts a time division multiplexing strategy to control opening of lasers with different wavelengths, and is matched with hardware handshake logic to force the camera to perform global shutter synchronous exposure. The upper computer builds a memory asynchronous buffer architecture, decouples high-bandwidth image acquisition and a low-speed hard disk storage process by using a producer-consumer model, and temporarily stores original data by using a memory buffer pool.

The utility model relates to the technical field of aluminum alloy production, in particular to an inverted microscope micro sample clamp for an aluminum alloy microscopic structure, and aims to solve the problems that in microscopic structure tests of thin profile, plate and wire aluminum alloy products and the like, a lamellar or thin rod-shaped aluminum alloy microscopic structure sample is difficult to place on an inverted microscope, and the test time is shortened. And the test efficiency is low. The clamp is composed of a sliding rod clamp frame, a left sliding block and a right sliding block, sliding rods are sleeved with the sliding blocks, the sliding blocks form a clamping device under the action of springs, baffles are fixed to the two ends of the sliding rods through hole sleeves, the sliding blocks abut against semicircular through holes in the middles of the two sliding blocks, and sample clamping is achieved. By means of the clamping device, the small aluminum alloy microscopic structure sample can be stably clamped, the problem that the thin-sheet-shaped and thin-rod-shaped aluminum alloy microscopic structure sample is difficult to place on an inverted microscope objective table is effectively solved, the test operation difficulty is greatly reduced, and the overall test efficiency is improved.

The invention relates to the technical field of microscopes, and discloses an inverted microscope which comprises a microscope base, an eyepiece used for human eyes to observe is arranged on the microscope base, an objective lens used for amplifying an object is rotatably connected to the microscope base, and an illuminating lamp used for improving brightness is arranged on the microscope base. The microscope base is fixedly connected with a transparent objective table used for placing a sample through a support, the transparent objective table is located over the objective lens and under the illuminating lamp, a cleaning assembly is arranged on the microscope base, a dust removal assembly is further arranged on the microscope base, and a sweeping assembly is arranged on the dust removal assembly. According to the objective table, the problem that the light transmission of the objective table is affected due to the fact that dust and impurities remaining on the objective table are difficult to remove is effectively avoided, meanwhile, floating dust on an objective lens can be removed in the process of cleaning the surface of the objective table, and the practicability of the objective table is improved.

The invention discloses a micro-fluidic chip special for bacterial biofilm culture and bottom in-situ observation, and belongs to the technical field of micro-fluidic chips. The micro-fluidic chip is composed of a glass substrate and a micro-channel main body formed by pouring polydimethylsiloxane, and after the glass substrate and the micro-channel main body are bonded through UV glue, a sunken micro culture chamber used for fixing bacteria and promoting the formation of a biological membrane is formed in the micro-channel main body. The micro-fluidic chip is compatible with an inverted microscope and a high-content imaging analysis system (such as Operetta CLS), and can be directly used for bottom observation. A sunken micro culture cavity is integrated in the chip and is used for fixing bacteria; the height of the microchannel connected with the outlet is low, bacteria can be limited in the cavity, meanwhile, a nutrient solution can be permitted to be continuously poured, and a stable fluid environment is provided for biofilm formation. According to the structural design, long-time and in-situ real-time observation and research of bacterial biofilm growth are realized.

The invention relates to a clay mineral hydration expansion dynamic characterization method. The invention relates to a clay mineral hydration expansion dynamic characterization method which comprises the following steps: constructing a micro-fluidic chip by adopting polydimethylsiloxane; pretreatment and loading of a clay sample: selecting clay minerals, drying, properly grinding and cutting, and filling the clay minerals into a sample chamber; a precise injection pump or a peristaltic pump is used for introducing water or a solution with specific chemical properties into the micro-fluidic chip at a controlled flow rate; the solution enters the sample chamber along the liquid inlet channel, infiltrates the clay minerals, and initiates the structure of the clay minerals to absorb water; the micro-fluidic chip is placed on an inverted microscope, real-time imaging is carried out through a transparent window, and image sequences of the expansion process of the clay minerals in the sample chamber are continuously collected at set time intervals. The method can capture and quantify the'non-uniform expansion behavior 'which cannot be recognized by the traditional method and continuously record the expansion process.

The application belongs to the technical field of medicine, and provides application of methylbadosolon in preparation of a medicine for inhibiting respiratory syncytial virus infection, and the application establishes an RSV infected cell and mouse model, observes pathological effects of BEAS-2B cells after infection of RSV by using an inverted microscope, detects cytotoxicity and antiviral effects of methylbadosolon by using a CCK8 method, detects RSV, IL-6, IL-8, IKKbeta and NF-kappa B mRNA expression by using a reverse transcription polymerase chain reaction, and detects RSV F, p-IKKbeta, p-NF-kappa B and IL-6 protein expression by using a Western blotting, for the first time, pharmacodynamics of methylbadosolon for relieving lung inflammation and injury caused by RSV infection through an IKKbeta / NF-kappa B pathway is confirmed, and methylbadosolon can be used for preparation of a medicine for inhibiting respiratory syncytial virus infection.

The present application relates to the field of parasitic detection method, more particularly, to a kind of etiology parasitic detection method of human body, comprising the following steps;Step 1, add clean water in the culture hole of cell culture plate, take a proper amount of feces and place in clean water and stir evenly;Step 2, the fecal liquid in the culture hole of cell culture plate is transferred to test tube, then the test tube is placed into centrifuge and centrifuged;Step 3, the supernatant in test tube is taken out and placed in another test tube;Step 4, repeat step 2 and step 3 until the fecal liquid is clear;Step 5, take the supernatant of step 4, collect pathogen;Step 6, the supernatant of step 5 is examined by inverted microscope, and the parasitic detection of human body is carried out.

The present invention relates to the technical field of biochips, and provides a sperm enrichment apparatus. The sperm enrichment apparatus comprises: an inverted microscope, comprising a stage; a high-speed CCD, disposed above the inverted microscope; a surface acoustic wave microfluidic chip, disposed on the stage and located below the high-speed CCD, and comprising a substrate and an interdigital electrode plated on the substrate; a signal generator, configured to generate ultrasonic waves; and an amplifier, configured to amplify the ultrasonic waves and then load the amplified ultrasonic waves to the surface acoustic wave microfluidic chip. The sperm enrichment apparatus, as a non-invasive method, has biocompatibility. The apparatus is mainly applied to raw sperm samples (including those with poor motility and no motility), and does not involve excessive manipulation of the sperm, thereby ensuring the naturalness of the fertilization process. The apparatus is reusable, simple to operate, and time-saving.

The invention relates to a variable curvature scanning lighting device for an inverted microscope and an imaging method thereof, the device comprises the inverted microscope and a flexible arc-shaped bracket located above the inverted microscope, and a plurality of LED light sources capable of independently addressing are uniformly arranged on the flexible arc-shaped bracket along the arc direction; the curvature adjusting mechanism is used for adjusting the curvature of the flexible arc-shaped bracket and changing the NA range of illumination of each LED light source; the rotating mechanism is configured to control the flexible arc-shaped bracket to rotate around the optical axis of the inverted microscope under the current curvature; the imaging method comprises the following steps: respectively executing complete rotary scanning under different curvatures by using the device, collecting a data set covering a plurality of NA ranges and all azimuth angles, and finally obtaining a high-quality image through calculation and reconstruction. Dual programmable control over the illumination angle is achieved, and the device is particularly suitable for deep optimization imaging of three-dimensional samples such as organoids and the like and has the remarkable advantages of being compact in structure and low in cost efficiency.

The present invention relates to a goniometer (angle measuring instrument) used to measure the polarization angle that affects the crystal orientation of a metal structure by rotating an inner ring with a scale. "Reference Figure 1 of Usage" shows the device with a fixing device for securing the sample stage attached. "Reference Figure 2 of Usage" shows the device with the sample stage set up. "Reference Figure 3 of Usage" shows the device with the sample mounted on the sample stage. The sample is mounted on the bottom side of the device and observed using an inverted microscope, as shown in "Reference Figure 4 of Usage".

The utility model relates to the technical field of inverted microscope accessories, in particular to an inverted microscope objective table heating device. The glass slide heating device comprises an objective table and a light transmitting opening formed in the objective table, a heating mechanism is assembled between the objective table and the light transmitting opening, the heating mechanism is used for heating a glass slide, and the heating mechanism comprises a bearing groove, an electric heating wire and a heat preservation layer. Through the structural design of the heating mechanism and the top heat preservation mechanism, heat preservation can be conveniently carried out on the end portion of the glass slide in the bearing groove, the heat preservation cover plate can cover the upper portion of the glass slide, invasion of external cold air is effectively isolated, the heat dissipation speed is greatly reduced, and the heat preservation effect is improved. And through the structural design of the adjusting mechanism, the device can quickly cover the heat preservation cover plate above the glass slide, and meanwhile, after observation is finished, the heat preservation cover plate can be conveniently and quickly removed from the upper part of the glass slide, so that use is facilitated.

The application discloses a surface-modified microparticle visual detection method. The preparation method adopts a liquid-phase co-coating method, modifies microparticles by using a surface modifier, and simultaneously uses a trace amount of dye as a dyeing agent to prepare dyed surface-modified microparticles; and the modification of the microparticle surface is observed by using a confocal fluorescence inverted microscope. The application establishes a visual detection method of a microparticle surface modifier, and can be widely applied to surface detection and research of various microparticles.

The utility model provides a fluorescent camera applied to an inverted microscope. The fluorescent camera comprises a mounting base and a moving assembly, the mounting base is provided with a mounting through groove, the mounting through groove is used for mounting the focus-shrinking photosensitive assembly, and the focus-shrinking photosensitive assembly is used for adjusting the focal length of optical imaging; the moving assembly is provided with a light path adjusting element, and the moving assembly is horizontally and movably mounted at the top of the mounting base; when the moving assembly moves to the position above the focus-shrinking photosensitive assembly, the light path adjusting element is added into an optical path between the objective lens and the ocular lens. The utility model has the technical effects of simple operation, convenient installation, and neat and beautiful appearance.

The invention discloses an X-ray microscopic system based on GAGG: Ce scintillator lens coupling, belongs to the technical field of X-ray microscopic imaging, and solves the problem that multi-mode imaging cannot be realized in the same X-ray microscopic system in the prior art. The X-ray microscopic imaging system comprises an X-ray source for irradiating a sample, and a linear light path type imaging system or an inverted microscope-based imaging system which is matched with the X-ray source to carry out X-ray microscopic imaging on the sample. The X-ray microscopic imaging device is used for X-ray microscopic imaging.

The invention discloses a two-dimensional material micro-spectrum measurement system under a variable temperature condition, which relates to the technical field of two-dimensional material spectrum detection, and comprises an excitation light source module, a light path transmission module, a variable temperature sample module, a spectrum acquisition module and a data processing module, the excitation light source module comprises a wide-spectrum halogen tungsten lamp and 532nm laser; the light path transmission module comprises an optical fiber, an objective lens, a pinhole, a collimating lens, a reflector and a focusing lens; the variable-temperature sample module comprises a cold and hot table inverted on the microscope objective table; the spectrum acquisition module is an optical fiber spectrometer, and a reflection mode is adopted during absorption spectrum measurement; the data processing module is used for carrying out Lorentz fitting on the acquired spectral data and calculating a lattice thermal expansion coefficient through the change of an absorption spectrum peak position; according to the invention, two measurement functions of absorption spectrum and fluorescence spectrum are integrated, measurement of different spectrum types can be realized through light source switching, the system does not need to be disassembled or reconstructed, and the measurement efficiency is improved.

The utility model discloses a multifunctional inverted micro-area optical measuring device, which comprises a bottom plate and a spectrograph, a multifunctional optical testing module is arranged at the top of the bottom plate, a Kohler lighting module is arranged on the multifunctional optical testing module, and an electric objective lens turntable is arranged at the top of the Kohler lighting module. A sample table is arranged on one side of the top of the Kohler illumination module, the electric objective lens rotating disc is located below the sample table, an XYZ axis is arranged in the sample table, a transmission light path module is arranged on the top of the sample table, and a circuit control module is arranged at one end of the back face of the sample table. According to the utility model, the top-down modular design idea of an inverted system is adopted, different compatible test modules can be established based on Kohler illumination, a user only needs to purchase a set of inverted microscope frame and a corresponding optical measurement function module to realize various different measurement requirements, and the whole set of equipment is high in cost performance, strong in openness and convenient to use. A user can expand and develop functions by himself / herself, the electric degree is high, and use is convenient.