Patents
Literature
Patsnap Eureka AI that helps you search prior art, draft patents, and assess FTO risks, powered by patent and scientific literature data.

67 results about "Inverted microscope" patented technology

An inverted microscope is a microscope with its light source and condenser on the top, above the stage pointing down, while the objectives and turret are below the stage pointing up. It was invented in 1850 by J. Lawrence Smith, a faculty member of Tulane University (then named the Medical College of Louisiana).

Liftable mechanism of inverted microscope condenser

The utility model provides a lifting mechanism of an inverted microscope condenser, which comprises a microscope main body, and a guide rail mechanism is arranged above the microscope main body; the lifting mechanism is arranged at the bottom of the guide rail mechanism; the collecting lens is mounted on the guide rail mechanism in a sliding manner through a collecting lens fixing bracket; the guide rail mechanism comprises a high-precision ball screw with a rotating gear at the bottom; the linear moving pairs are arranged on the left side and the right side of the high-precision ball screw; the lifting mechanism comprises a self-locking worm and gear pair meshed with the rotating gear; the rolling bearing is connected with the outer side of the self-locking worm and gear pair; the lifting rotating shaft is connected with the outer side of the rolling bearing; the lifting knob is connected with the outer side of the lifting rotating shaft. The device is easy and convenient to operate and high in efficiency, and the collecting lens can be adjusted to any height.
Owner:SUZHOU FLUOMED TECH CO LTD

Liquid drop sorter based on fluorescence detection

According to the liquid drop sorting instrument based on fluorescence detection, an inverted microscope serves as a whole instrument base and is located at the bottom, an XY precise moving platform is fixed to the microscope, a z-axis moving platform is fixed to a simple supporting frame beside the microscope, a fluorescence channel is installed on the z-axis moving platform, and a microfluidic liquid drop sorting module is fixed to the tip end of the fluorescence channel. A fluorescence detection module is arranged on the side wall of the fluorescence channel, the fluorescence detection module and the microfluidic liquid drop sorting module are both connected with the signal end of the FPGA signal processing module, the fluorescence detection module and the microfluidic liquid drop sorting module are controlled through the FPGA, the condition of generated liquid drops is observed through a microscope, the sorting process of the liquid drops is dynamically adjusted, and the quality of the liquid drops is improved. The closed-loop control of fluorescent liquid drop sorting is realized; according to the invention, high-precision and high-throughput liquid drop sorting can be realized, the liquid drop screening efficiency is greatly improved, the target liquid drop can be accurately identified and selected, and the method can be widely applied to the fields of high-throughput screening, single cell analysis, enzyme evolution, drug discovery and the like.
Owner:XI AN JIAOTONG UNIV

Prism-type total internal reflection imaging apparatus compatible with commercial inverted microscope, and method for using same

Disclosed in the present invention are a prism-type total internal reflection imaging apparatus compatible with a commercial inverted microscope, and a method for using same. The method comprises the following steps: first injecting a sample into a sample cell; turning on a laser, and generated evanescent waves illuminating nearby fluorescent molecules to obtain an image; and processing the image to obtain the three-dimensional positions of all the fluorescent molecules in a visual field, obtaining the three-dimensional trajectories of the fluorescent molecules, calculating a mean square end-to-end distance and a diffusion factor of the trajectories, and obtaining a relative potential energy distribution curve on the basis of the relative positions of the molecules. The present invention can be compatible with a mainstream commercial inverted microscope, and does not need to carry out any refit on existing microscopes. A sample stage is mounted on the inverted microscope and an excitation light path module is mounted on the side edge of the sample stage, so that a total internal reflection light path having a specific wavelength can be generated. The apparatus can achieve single molecule positioning and three-dimensional tracking with a high signal-to-noise ratio, and the sample cell can be flexibly replaced before each test without influencing the total internal reflection light path. The apparatus is applied to the fields of life science, material analysis and the like.
Owner:SOUTH CHINA UNIV OF TECH

3D cell clone culture screening method and screening system

The invention relates to a 3D cell clone culture screening method and system, and the method comprises the following steps: obtaining an image of a clone cell to be selected through an inverted microscope, and calculating the position information of the clone cell to be selected in X and Y directions; obtaining the Z-axis position of an inverted microscope focusing mechanism when the clone cell needing to be selected is clearest in the inverted microscope and the Z-axis position of the inverted microscope focusing mechanism when the bottom of the culture hole is calibrated, and calculating the position information z of the clone cell needing to be selected away from the bottom of the culture hole; according to the position information, a three-axis moving mechanism is used for driving a pipettor to suck clone cells needing to be selected, and the clone cells are transferred into a clone receiving plate. The height position of the clone cell is calibrated through the focusing distance of the inverted microscope, so that the clone cell can be accurately selected, the accuracy of cell screening is ensured, and the method has very high universality, universality and use value.
Owner:SUZHOU ZHONGYAN BIO-INFORMATION CO LTD

Monomolecular observation and function research platform based on nanopore and detection method

The invention discloses a nanopore-based single molecule observation and function research platform and a detection method, and belongs to the technical field of single molecule observation and detection. The invention discloses a nanopore-based single molecule observation and function research platform, which comprises an inverted microscope system, a single molecule observation and function research assembly, an openable electromagnetic shielding cover, a control system, an upper computer, a display system and a shockproof platform. Researchers can perform observation, detection and related function research work on a single molecule by using the platform, especially can realize observation, detection and related function research on DNA molecules and protein molecules, and the invention provides a novel detection platform for single molecule research, which is used in the fields of function research, sequencing and the like of the single molecule and has a wide application prospect. And position imaging, length detection and characteristic structure analysis of a single long-chain molecule are realized.
Owner:CHONGQING INST OF GREEN & INTELLIGENT TECH CHINESE ACAD OF SCI

Method for culturing fungal-eating nematodes by mixing two fungi and reducing pollution of culture medium

The invention discloses a method for culturing fungal-feeding nematodes by using two fungi in a mixed manner and reducing pollution of a culture medium. The method comprises the following steps: A, separating and extracting euphorbia fungal-feeding nematodes; b, identifying the euphorbia fungal nematodes; c, preparing a strain mixture which comprises botrytis cinerea and trichoderma harzianum in a mass ratio of 1: 1; d, inoculating the strain mixture into a PDA liquid culture medium to prepare a nematode PDA culture medium; e, centrifuging the nematodes obtained in the step B; f, disinfecting the nematodes, namely disinfecting the surfaces of the nematodes by using a mixed solution of streptomycin sulfate and actinomycete ketone; g, adding sterile water into the nematodes disinfected in the step F, and centrifuging; h, repeating the step G for multiple times to obtain nematode liquid; i, inoculating the disinfected nematodes into a flat plate of a PDA solid culture medium; and J, observing the growth quantity of the nematodes in the culture medium by using an inverted microscope. According to the method, the nematodes are cultured by culturing the fungus-eating nematode strain mixture, and the method is used for culturing and propagating the nematodes.
Owner:CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY

Cell culture counting plate

The utility model discloses a cell culture counting plate which comprises a base and an upper cover, a lower groove is formed in the base, a mixed solution is contained in the lower groove, a sliding groove is formed in the base, one end of the sliding groove is closed, the other end of the sliding groove is open, and a connecting block is correspondingly arranged at the bottom of the upper cover. One side of the upper cover is connected with an extension seat, supporting legs are arranged at the bottom of the extension seat, second pulleys are arranged at the bottoms of the supporting legs, an upper groove is formed in the upper cover, a slide is arranged in the upper groove, and the upper cover is connected to the base in a sliding mode and plays a role in sealing the base; when the cell culture counting plate is used, the mixed solution in the lower groove is dropped on the slide through the dropper, then the slide in the lower groove of the cell culture counting plate is aligned and placed under a lens of an inverted microscope, the number of cells in the sample solution is obtained, and finally the number of cells in the mixed solution is calculated through a counting formula.
Owner:PNAS CELL (XIAMEN) BIOTECHNOLOGY CO LTD

A sectioning method and imaging method for high-resolution imaging of intact thick biological tissue

The application discloses a sectioning method and imaging method for high-resolution imaging of intact thick biological tissues, and the sectioning method comprises the following steps: S1, placing a cover glass on a first object slide and covering both with adhesive tape; S2, digging off the adhesive tape covered in the area of the cover glass, and processing the edges of the dug-off area with a hydrophobic medium; S3, transferring a tissue sample to be observed to the cover glass, absorbing the sample liquid, adding an anti-fluorescence quencher dropwise, absorbing the sample solvent after standing, adding gel dropwise, and fixing the sample on the cover glass; S4, removing the cover glass and placing it on a second object slide, so that the tissue sample is located between the cover glass and the second object slide, and the cover glass is fixedly connected with the second object slide through the adhesive tape, and the sectioning is completed. The application can observe the distribution and quantity of specific cells in thick tissues at high resolution while keeping the integrity of the tissues, and the preparation method is simple, is not limited by light paths, sizes and the like, and is suitable for most upright or inverted microscopes.
Owner:QINGPU BRANCH OF ZHONGSHAN HOSPITAL AFFILIATED TO FUDAN UNIV (SHANGHAI QINGPU DISTRICT CENT HOSPITAL)

Inverted microscope capable of rapidly switching samples

The utility model relates to an inverted microscope capable of rapidly switching samples, which comprises a microscope main body, a sliding chute arranged on the microscope main body, a switching frame sliding in and out of the sliding chute, and at least two groups of fixing units arranged on the switching frame and used for fixing glass slides, the fixing unit comprises a sliding piece which is arranged in the switching frame and slides upwards in an inclined mode, an edge pressing piece is hinged to the upper end of the sliding piece, and the hinged position is located in the middle of the edge pressing piece. In the fixing process, the sliding part is pushed by the driving structure to realize the process of firstly lifting and then pressing, on the contrary, when the glass slides are not fixed, the sliding part and the edge pressing part are contracted in the switching frame, shielding cannot be formed to affect replacement of the glass slides, when one glass slide is observed, the other glass slide can be prepared in advance, and the time of observing the other glass slide is shortened. And the observation efficiency is greatly improved.
Owner:YIZHENG (SHANGHAI) BIOTECHNOLOGY CO LTD

Visual nanoneedle transfection micro-fluidic chip, preparation method and cell transfection method

The embodiment of the invention discloses a visual nanoneedle transfection micro-fluidic chip, a preparation method and a cell transfection method.The micro-fluidic chip comprises a patterned glass nanoneedle substrate and a transparent PDMS micro-channel layer, the transparent PDMS micro-channel layer is bonded to the patterned glass nanoneedle substrate, and the transparent PDMS micro-channel layer is bonded to the patterned glass nanoneedle substrate. The patterned glass nanoneedle substrate and the transparent PDMS micro-channel layer jointly form a light-transmitting structure and are compatible with a fluorescent inverted microscope; wherein the surface of the patterned glass nanoneedle substrate is provided with a nanoneedle array of which the pinpoint height range is 700-1000nm and the pinpoint diameter range is 50-200nm; the transparent PDMS micro-channel is a micro-channel prepared from a high-transparency PDMS material, and the micro-channel comprises a channel main body, a channel inlet formed in one end of the channel main body and a channel outlet formed in the other end of the channel main body; wherein the height range of a flow channel in the flow channel main body is 10-25 microns, the width range of the flow channel is 10-100 microns, and a flow channel inlet is used for being connected with an external micro-flow pump.
Owner:QINGZHI BIOTECHNOLOGY (XUZHOU) CO LTD

Inverted microscope

This invention relates to the field of microscopy and discloses an inverted microscope, including a base with an eyepiece for human observation, an objective lens for magnifying objects rotatably connected to the base, an illumination lamp for increasing brightness, and a transparent stage for placing samples, positioned directly above the objective lens and below the illumination lamp, all fixedly connected to the base via a bracket. The base also includes a cleaning assembly, a dust removal assembly, and a sweeping assembly. This invention effectively avoids the problem of difficulty in removing dust and impurities remaining on the stage, which can affect the stage's light transmittance. Furthermore, the cleaning process on the stage surface also removes dust from the objective lens, improving the device's practicality.
Owner:ZHIHE BIOLOGICAL (GUANGZHOU) CO LTD

An inverted microscope objective

The invention discloses an inverted microscope objective lens, which includes a first lens group and a second lens group; the first lens group includes a first lens with positive refractive power, the second lens group has positive refractive power, and the second lens group includes a second lens, a third lens, a fourth lens, a fifth lens, a sixth lens, a seventh lens and an eighth lens in sequence, the second lens has positive refractive power, the third lens has negative refractive power, the fourth lens has positive refractive power, the fifth lens has positive refractive power, the sixth lens has negative refractive power, the seventh lens has positive refractive power, the eighth lens has negative refractive power, and the second lens group has at least two groups of cemented lenses. The invention includes a first lens group and a second lens group along the direction from the object side to the image side, and by designing eight lenses accordingly, the inverted objective lens optical system has the characteristics of large magnification, long working distance, large numerical aperture, high resolution performance, small chromatic aberration, large field of view, small number of lenses, and short total length of the optical system.
Owner:MOTIC CHINA GROUP CO LTD

CO2-water / salt water displacement interface instability characterization system and method based on microfluidic technology

PendingCN121954987ACompensate for the inability to simulate real underground reservoir conditionsRealize real-time visual observationFlow propertiesMaterial analysis by optical meansCapillary TubingEngineering
The invention provides a CO2-water / salt water displacement interface instability characterization system and method based on a microfluidic technology, and belongs to the field of carbon dioxide salt water layer sealing. According to the invention, a micro-fluidic chip with specific pore structure characteristics is designed, a micro-fluidic visual experiment system based on an inverted microscope is established, and characterization parameters including capillary number, viscosity ratio, fractal dimension and the like are extracted by carrying out digital processing on continuously collected image sequences; the interface evolution characteristics of CO2 displacement water / salt water are described through the capillary number and the viscosity ratio, and the interface complexity is quantitatively described by means of fractal dimensions so as to reflect the instability of the interface. According to the method, the interface instability in the displacement process is converted into a numerical value capable of being accurately predicted from traditional qualitative description, the influence mechanism of the pore structure, the viscosity ratio, the capillary tube number and the like on the interface instability can be visually revealed, and key data support is provided for CO2 geological sequestration evaluation.
Owner:XINJIANG UNIVERSITY

Separating, screening and sucking device for cells

The utility model relates to a separating, screening and sucking device for cells. The separating, screening and sucking device comprises a rotary sucking module, a separating and screening module, a visual detection module and a discharging and carrying module, the rotary suction module comprises a rotary manipulator module and a suction module arranged at the tail end of a mechanical arm of the rotary manipulator module; the separating and screening module comprises an objective table and an inverted microscope module arranged below the objective table; the visual detection module is used for guiding and positioning the rotary suction module and realizing displacement compensation; after the separating and screening module completes screening through vision, the rotary suction module sucks the microorganisms to the discharging and carrying module. Compared with manual cell selection, the efficiency of manual cell extraction can be improved; operations such as boring cell selection and easy errors are omitted, and the yield of manual cell selection is improved; and the device has strong universality, universality and use value in different fields and different products.
Owner:BEIJING FOCUSIGHT TECH

A high-speed atomic force microscope scanning method based on visual guidance

The application provides a high-speed atomic force microscope scanning method based on visual guidance, which comprises the following steps: a direct correlation model of regional gray scale variation and the end position of a scanning mechanism is established, and a magnetic hysteresis compensation strategy based on a PI inverse model is given, so that accurate servo control of a complex scanning track can be realized on the basis of low visual delay; and a device for realizing the method comprises an atomic force microscope, a flexible nanometer positioning platform, a general positioning platform, an inverted microscope, a camera, a sliding table, an inverted microscope clamp, a platform support, a computer and a visual servo control system. The atomic force microscope scanning control method and device provided by the application have the advantages of high upper limit of scanning frequency, high scanning precision and high efficiency in improving the scanning performance of an existing atomic force microscope.
Owner:SOUTH CHINA UNIV OF TECH

Inverted microscope

To provide an inverted microscope equipped with an immersion liquid supply device capable of easily supplying immersion liquid even during observation.SOLUTION: An inverted microscope comprises: a liquid immersion objective lens 102a; a dry objective lens 102b; a rotary revolver 103 on which a plurality of objective lenses are mountable and which rotates to switch the objective lenses; a support base 100; a focus mechanism 104 which moves the rotary revolver 103 in an optical axis O3a direction with respect to the support base 100; an immersion liquid supply base 11a fixed to the support base 100; an immersion liquid supply unit 11b which includes an immersion liquid supply tube 11c that is movable in an optical axis direction with respect to the immersion liquid supply base 11a and supplies immersion liquid to the liquid immersion objective lens; and transmission mechanisms (11ba, 11a) which allow transmission of movement of the focus mechanism in the optical axis direction to the immersion liquid supply unit when the liquid immersion objective lens is arranged within a focus range on the optical axis, and releases transmission of movement of the focus mechanism in the optical axis direction to the immersion liquid supply unit when the liquid immersion objective lens is arranged in a downward region located on the optical axis and outside the focus range.SELECTED DRAWING: Figure 2
Owner:EVIDENT CORP

Single cell cloning and picking device

The utility model relates to the related technical field of single cell picking devices, in particular to a single cell clone picking device, which is used for picking single cells in a cell culture dish, the cell picking culture dish is positioned in a picking area of an inverted microscope objective table, and the single cell clone picking device is used for picking the single cells in the cell culture dish. Microscope collecting lenses with fixed positions are arranged above the picking area at intervals, and microscope collecting lenses are arranged above the picking area of the inverted microscope objective table at intervals; cells at the bottom of the culture dish are scraped off through the cell suction tube opening, the cells are sucked and stored in the cell suction tube by adjusting the position of the rubber plug through the suction adjusting assembly, the sucked cells can be pushed into a new culture dish containing a culture medium, and picking and transferring of single cell clones are achieved. The device is easy to assemble and disassemble, and is suitable for most inverted microscopes, and the cell suction tube opening covers single cell cloning, so that other parenchyma cells cannot be doped in the picking process, and the unicity of single cell cloning is ensured.
Owner:DENTAL HOSPITAL AFFILIATED TO GUANGXI MEDICAL UNIV (DENTAL HOSPITAL OF GUANGXI ZHUANG AUTONOMOUS REGION)

Low-density sperm braking method without PVP medium in ICSI fertilization

The invention discloses a low-density sperm braking method without a PVP medium in ICSI fertilization, and relates to the field of assisted reproduction therapy, and the method comprises the following steps: step 1, diluting a sperm suspension until the density is 0.1 * 10 < 6 > / ml, and placing the diluted sperm suspension in an in-vitro operating solution without PVP; step 2, under an inverted microscope, directly pressing and braking the tail of the sperm in the in-vitro operating fluid by using a microinjection needle, so that the tail of the sperm is folded or curled to deform; and step 3, transferring the braked sperms into operation liquid drops containing ovum to complete microinjection, and performing tail pressing operation in an isotonic operation liquid without PVP (Polyvinyl Pyrrolidone), so that the tail parts of the sperms present visible curled deformation or tail folding. The sperm motility destruction rate is 100% (residual motility is completely prevented from interfering fertilization), and the tail cell membrane rupture rate is 100% (it is ensured that activation factors in sperms are fully released).
Owner:随刘才

Fluorescent biosensing analysis device and method

Femtosecond pulse near-infrared Gaussian laser is adopted as a unified light source and is simultaneously used for time-sharing scanning optical tweezers capturing and two-photon fluorescence excitation, and a light path part is sequentially provided with a height adjusting assembly, a galvanometer scanning element, a beam expanding assembly and a telescope assembly. The light is reflected by a dichroscope configured on an inverted microscope platform, strong focusing is realized in a PDMS sample chamber by a high numerical aperture objective lens, bright field illumination is provided by a white light LED lamp, and a two-photon fluorescence signal is collected by a CMOS camera; a high-precision biosensing system based on a CRISPR / Cas12a system is constructed on the surface of a microsphere enriched with a detection signal output unit, quenching-recovery conversion of a fluorescence signal is realized, and two-photon fluorescence imaging is carried out in a multiple optical capture microsphere array formed by time-sharing scanning optical tweezers. The method has the advantages of high detection accuracy and sensitivity, low background signal, good imaging stability, strong anti-interference capability in a complex biological medium and the like.
Owner:WUHAN UNIV OF SCI & TECH

Multi-objective switching device for microscope

The utility model discloses a multi-objective switching device for a microscope, which comprises an objective module and a driving mechanism, the driving mechanism comprises a linear driving mechanism and a rotary driving mechanism, the linear driving mechanism is used for driving the objective module to move towards or away from the linear driving mechanism, and the rotary driving mechanism comprises a rotating shaft. The objective lens module comprises a plurality of objective lenses and objective lens mounting pieces; the objective lens mounting pieces are connected with the rotating shaft; the objective lens mounting part is mounted around the rotating shaft, one side, far away from the rotating shaft, of the objective lens mounting part is detachably connected with at least two objective lenses, and magnification times of the objective lenses are different. According to the multi-objective switching device for the microscope, the switching of the objective can be realized by utilizing the rotation of the rotating shaft, and the included angle between the objective can be adjusted according to the actual situation, so that the adjustment of the number of the objective installed by the objective installation piece can be realized, and the whole structure is compact and small, and can be applied to an inverted microscope system.
Owner:APPLITECH BIOLOGICAL TECH CO LTD

Microscope Enabled 3D Bioprinting System

An apparatus controls a series of actuators mounted onto inverted microscope assemblies to add 3D bioprinting and pick and place capabilities on a microscopic scale. One or more syringes are also mounted to the apparatus and the plunger of each syringe is independently actuated to extrude or extract fluids from the optically aligned needle. A software package communicates with the microscope x, y stage as well as the on-board motor controller which actuates the syringes and needle to set the x, y, z position and syringe in real time.
Owner:WISCONSIN ALUMNI RES FOUND

Mouse live imaging fixation device based on inverted microscope

The utility model relates to observation device technical field belongs to a kind of mouse live imaging fixing device based on inverted microscope, including fixed on the fixed device box base body and base of microscope platform, the fixed device box base body bottom is fixedly connected on base, the upper portion of fixed device box base body is equipped with detachable seal cover, the base is opened on the observation window, the fixed device box base body perimeter is also opened experimental fixed hole. The utility model can flexibly match the tissue of surgical exposure mouse and the mouse of surgical installation observation window are observed using inverted microscope, reduce the development cost of multiple sets of fixed device, can also achieve certain mouse physiological state maintenance function, and compatible various equipment required by experiment, simplify live imaging observation procedure.
Owner:ZHEJIANG HEHU TECH CO LTD

An illumination device for solving the concave liquid surface effect in orifice plate imaging

This invention discloses an illumination device for solving the concave liquid surface effect in well plate imaging, belonging to the field of microscope light source technology. The device includes an inverted microscope illumination source and several annular light sources distributed in a dome-like pattern around it. The inverted microscope illumination source is composed of a central light source, a collimating lens, and a condenser lens in sequence. The annular light sources contain multiple LED beads, and the size of the rings matches the diameter of the microwells in a common cell culture plate, with adjustable angles. Through the synergistic effect of traditional vertical illumination and dome-shaped oblique illumination, this invention effectively improves the imaging quality of well plates, adapts to microwell plates of different diameters, is easy to operate, and highly practical, providing strong support for cell observation and other related work.
Owner:CHONGQING LIANQING RUIQI TECH CO LTD

Scanning ionic conductance microscope platform for single cell scanning and detection

ActiveCN223769998UMaterial impedanceScanning ion-conductance microscopyPetri dish
The utility model belongs to the technical field of scanning ionic conductance microscopes, and discloses a scanning ionic conductance microscope platform for single cell scanning and detection, which comprises an inverted microscope body, a culture dish tray of the inverted microscope body is detachably connected with a protection device, and the protection device is internally provided with an accommodating cavity for embedding a culture dish. The containing cavity extends to the bottom of the protection device, a first observation hole communicated with the top of the containing cavity is formed in the top of the protection device, a transparent cover plate is arranged on the upper side of the first observation hole, and a second observation hole is formed in the transparent cover plate; a carbon dioxide concentration detection device is arranged in the containing cavity, a carbon dioxide input hole and a carbon dioxide concentration detection device wire passing hole are further formed in the protection device, and the protection device is connected with a carbon dioxide supply device through the carbon dioxide input hole. According to the scanning ion conductance microscope platform, on the basis of an existing scanning ion conductance microscope platform, the gas environment of cells during in-vitro culture can be guaranteed, and meanwhile target cells can be conveniently found.
Owner:XI AN JIAOTONG UNIV

A method for detecting human parasitic diseases without using a glass slide and a cover glass

This invention relates to the field of fecal testing, and more specifically, to a method for detecting human parasitic pathogens without the use of slides and coverslips, comprising the following steps: Step 1, adding an appropriate amount of water to the culture wells of a cell culture plate, placing an appropriate amount of feces in the water of the culture well and stirring evenly; Step 2, cutting a piece of textile or nylon mesh of similar size to the culture well and placing it into the culture well to isolate the fecal residue and fecal liquid from each other; Step 3, after stirring and mixing the fecal liquid, transferring the fecal liquid to the culture wells of a new cell culture plate, covering it with the cell plate cover, and examining it under a microscope using an inverted microscope.
Owner:HAINAN MEDICAL UNIV

Sperm identification method, system and device, computer equipment and readable storage medium

The invention relates to a sperm identification method, system and device, computer equipment and a readable storage medium. The method comprises the following steps: in response to a sperm identification instruction, controlling a three-axis electric control objective table to move a culture dish placed on the objective table in spaces in X, Y and Z directions of an inverted microscope according to a preset scanning path, so that the inverted microscope scans sperm observation liquid in the culture dish, obtaining a microscopic image of the sperm observation liquid; taking the microscopic image as input, calling a trained sperm identification model, identifying sperms in the microscopic image, and obtaining a sperm identification result, the trained sperm identification model being obtained by training based on a historical microscopic image of category labels and position information of a real frame carrying sperms; and determining the spatial position of each identified sperm based on the sperm identification result. By adopting the method, the sperm identification efficiency can be improved.
Owner:REPRODUCTIVE & GENETIC HOSPITAL OF CITIC XIANGYA CO LTD

Fluid immersion control for inverted microscopy

Inverted microscope system (100), comprising: a control system (150) for fluid immersion for inverted microscopy, wherein the control system comprises: a sensor ring (104) coupled to a distal region of a microscope lens used with an inverted microscope (120), wherein the sensor ring (104) forms a first ring in fluid communication with the microscope lens, the first ring being able to convey a fluid that forms an immersion layer on a surface of the microscope lens during imaging using the microscope lens, and wherein the sensor ring (104) further comprises: a plurality of sensor electrodes (306) arranged circumferentially around the first ring and defining a first diameter, wherein each sensor electrode of the plurality of sensor electrodes (306) has an arc shape, and wherein each of the plurality of sensor electrodes remains in contact with the fluid when the microscope lens is immersed in the fluid; a common electrode (304) arranged as a second ring within the first ring, the common electrode remaining in fluid communication with the fluid; and a fluid port (612) for receiving additional fluid to replenish the immersion layer; a fluid refilling system (208) coupled to the fluid connection and capable of providing the additional fluid; an immersion controller (206) coupled to the plurality of sensor electrodes (306), the common electrode (304) and the fluid refill system (208), wherein the immersion controller is capable of: when the microscope lens is immersed in the fluid, a first resistance of the fluid is measured between each of the plurality of sensor electrodes (306) and the common electrode (304) in order to detect the fluid at each respective sensor electrode of the plurality of sensor electrodes; when the fluid exposes a first sensor electrode, which is contained in the plurality of sensor electrodes, measuring a second resistance of an air gap at the first sensor electrode; and in response to the detection of the air gap, controlling the fluid replenishment system (208) to provide the additional fluid, wherein the immersion control (206) is able to provide a fluid and thereby immerse the microscope lens at least up to the first diameter, the first diameter being larger than a second diameter corresponding to an optical axis (114) of the microscope lens.
Owner:IDEX HEALTH & SCIENCE LLC

Neuroelectrophysiological detection and stimulation platform

ActiveCN309444614SSomaPhysical therapy
1. The name of this design product: Neuroelectrophysiological detection and stimulation platform. 2. Purpose of this product design: This product design is used for neuroelectrophysiological detection and stimulation of isolated cells and can be used in conjunction with an inverted microscope. 3. The key point of the design of this product lies in its shape. 4. The picture or photo that best illustrates the design points: Stereoscopic drawing 1.
Owner:SHENZHEN HUADA GENE INST

Aluminum-plastic interface in-situ morphology and spectrum combined analysis system and analysis method in hydrothermal environment

The invention discloses an aluminum-plastic interface in-situ morphology and spectrum combined analysis under a hydrothermal environment, which comprises a sample holder, an in-situ heating table is arranged on the sample holder, a hydrothermal reactor for placing a sample is arranged in the in-situ heating table, observation windows are arranged on the upper side and the lower side of the in-situ heating table, and the in-situ heating table is connected with the hydrothermal reactor. An inverted microscope is arranged below an observation window on the lower side of the in-situ heating table, a camera is arranged at an observation port of the inverted microscope, and a Fourier infrared ATR spectrometer and a Raman spectrometer are arranged above the observation window on the upper side of the in-situ heating table; the camera, the Fourier infrared ATR spectrometer and the Raman spectrometer are respectively connected with the server, and the server is sequentially connected with the machine learning module and the visual report module. The aluminum-plastic interface in-situ morphology and spectrum combined analysis system under the hydrothermal environment has the problems of insufficient real-time monitoring and incomplete data analysis in the current research.
Owner:XIAN UNIV OF TECH

Inverted Microscope (PX55)

1. Name of the Design Product: Inverted Microscope (PX55). 2. Use of the Design Product: An instrument for magnifying tiny objects. 3. Design Key Points of the Design Product: Lies in the shape. 4. Picture or Photograph that Best Illustrates the Design Key Points: Perspective view.
Owner:MOTIC CHINA GROUP CO LTD