52 results about "Inverted microscope" patented technology

According to the liquid drop sorting instrument based on fluorescence detection, an inverted microscope serves as a whole instrument base and is located at the bottom, an XY precise moving platform is fixed to the microscope, a z-axis moving platform is fixed to a simple supporting frame beside the microscope, a fluorescence channel is installed on the z-axis moving platform, and a microfluidic liquid drop sorting module is fixed to the tip end of the fluorescence channel. A fluorescence detection module is arranged on the side wall of the fluorescence channel, the fluorescence detection module and the microfluidic liquid drop sorting module are both connected with the signal end of the FPGA signal processing module, the fluorescence detection module and the microfluidic liquid drop sorting module are controlled through the FPGA, the condition of generated liquid drops is observed through a microscope, the sorting process of the liquid drops is dynamically adjusted, and the quality of the liquid drops is improved. The closed-loop control of fluorescent liquid drop sorting is realized; according to the invention, high-precision and high-throughput liquid drop sorting can be realized, the liquid drop screening efficiency is greatly improved, the target liquid drop can be accurately identified and selected, and the method can be widely applied to the fields of high-throughput screening, single cell analysis, enzyme evolution, drug discovery and the like.

Disclosed in the present invention are a prism-type total internal reflection imaging apparatus compatible with a commercial inverted microscope, and a method for using same. The method comprises the following steps: first injecting a sample into a sample cell; turning on a laser, and generated evanescent waves illuminating nearby fluorescent molecules to obtain an image; and processing the image to obtain the three-dimensional positions of all the fluorescent molecules in a visual field, obtaining the three-dimensional trajectories of the fluorescent molecules, calculating a mean square end-to-end distance and a diffusion factor of the trajectories, and obtaining a relative potential energy distribution curve on the basis of the relative positions of the molecules. The present invention can be compatible with a mainstream commercial inverted microscope, and does not need to carry out any refit on existing microscopes. A sample stage is mounted on the inverted microscope and an excitation light path module is mounted on the side edge of the sample stage, so that a total internal reflection light path having a specific wavelength can be generated. The apparatus can achieve single molecule positioning and three-dimensional tracking with a high signal-to-noise ratio, and the sample cell can be flexibly replaced before each test without influencing the total internal reflection light path. The apparatus is applied to the fields of life science, material analysis and the like.

The invention relates to a 3D cell clone culture screening method and system, and the method comprises the following steps: obtaining an image of a clone cell to be selected through an inverted microscope, and calculating the position information of the clone cell to be selected in X and Y directions; obtaining the Z-axis position of an inverted microscope focusing mechanism when the clone cell needing to be selected is clearest in the inverted microscope and the Z-axis position of the inverted microscope focusing mechanism when the bottom of the culture hole is calibrated, and calculating the position information z of the clone cell needing to be selected away from the bottom of the culture hole; according to the position information, a three-axis moving mechanism is used for driving a pipettor to suck clone cells needing to be selected, and the clone cells are transferred into a clone receiving plate. The height position of the clone cell is calibrated through the focusing distance of the inverted microscope, so that the clone cell can be accurately selected, the accuracy of cell screening is ensured, and the method has very high universality, universality and use value.

The invention discloses a method for culturing fungal-feeding nematodes by using two fungi in a mixed manner and reducing pollution of a culture medium. The method comprises the following steps: A, separating and extracting euphorbia fungal-feeding nematodes; b, identifying the euphorbia fungal nematodes; c, preparing a strain mixture which comprises botrytis cinerea and trichoderma harzianum in a mass ratio of 1: 1; d, inoculating the strain mixture into a PDA liquid culture medium to prepare a nematode PDA culture medium; e, centrifuging the nematodes obtained in the step B; f, disinfecting the nematodes, namely disinfecting the surfaces of the nematodes by using a mixed solution of streptomycin sulfate and actinomycete ketone; g, adding sterile water into the nematodes disinfected in the step F, and centrifuging; h, repeating the step G for multiple times to obtain nematode liquid; i, inoculating the disinfected nematodes into a flat plate of a PDA solid culture medium; and J, observing the growth quantity of the nematodes in the culture medium by using an inverted microscope. According to the method, the nematodes are cultured by culturing the fungus-eating nematode strain mixture, and the method is used for culturing and propagating the nematodes.

The application discloses a sectioning method and imaging method for high-resolution imaging of intact thick biological tissues, and the sectioning method comprises the following steps: S1, placing a cover glass on a first object slide and covering both with adhesive tape; S2, digging off the adhesive tape covered in the area of the cover glass, and processing the edges of the dug-off area with a hydrophobic medium; S3, transferring a tissue sample to be observed to the cover glass, absorbing the sample liquid, adding an anti-fluorescence quencher dropwise, absorbing the sample solvent after standing, adding gel dropwise, and fixing the sample on the cover glass; S4, removing the cover glass and placing it on a second object slide, so that the tissue sample is located between the cover glass and the second object slide, and the cover glass is fixedly connected with the second object slide through the adhesive tape, and the sectioning is completed. The application can observe the distribution and quantity of specific cells in thick tissues at high resolution while keeping the integrity of the tissues, and the preparation method is simple, is not limited by light paths, sizes and the like, and is suitable for most upright or inverted microscopes.

The utility model relates to an inverted microscope capable of rapidly switching samples, which comprises a microscope main body, a sliding chute arranged on the microscope main body, a switching frame sliding in and out of the sliding chute, and at least two groups of fixing units arranged on the switching frame and used for fixing glass slides, the fixing unit comprises a sliding piece which is arranged in the switching frame and slides upwards in an inclined mode, an edge pressing piece is hinged to the upper end of the sliding piece, and the hinged position is located in the middle of the edge pressing piece. In the fixing process, the sliding part is pushed by the driving structure to realize the process of firstly lifting and then pressing, on the contrary, when the glass slides are not fixed, the sliding part and the edge pressing part are contracted in the switching frame, shielding cannot be formed to affect replacement of the glass slides, when one glass slide is observed, the other glass slide can be prepared in advance, and the time of observing the other glass slide is shortened. And the observation efficiency is greatly improved.

The embodiment of the invention discloses a visual nanoneedle transfection micro-fluidic chip, a preparation method and a cell transfection method.The micro-fluidic chip comprises a patterned glass nanoneedle substrate and a transparent PDMS micro-channel layer, the transparent PDMS micro-channel layer is bonded to the patterned glass nanoneedle substrate, and the transparent PDMS micro-channel layer is bonded to the patterned glass nanoneedle substrate. The patterned glass nanoneedle substrate and the transparent PDMS micro-channel layer jointly form a light-transmitting structure and are compatible with a fluorescent inverted microscope; wherein the surface of the patterned glass nanoneedle substrate is provided with a nanoneedle array of which the pinpoint height range is 700-1000nm and the pinpoint diameter range is 50-200nm; the transparent PDMS micro-channel is a micro-channel prepared from a high-transparency PDMS material, and the micro-channel comprises a channel main body, a channel inlet formed in one end of the channel main body and a channel outlet formed in the other end of the channel main body; wherein the height range of a flow channel in the flow channel main body is 10-25 microns, the width range of the flow channel is 10-100 microns, and a flow channel inlet is used for being connected with an external micro-flow pump.

This invention relates to the field of microscopy and discloses an inverted microscope, including a base with an eyepiece for human observation, an objective lens for magnifying objects rotatably connected to the base, an illumination lamp for increasing brightness, and a transparent stage for placing samples, positioned directly above the objective lens and below the illumination lamp, all fixedly connected to the base via a bracket. The base also includes a cleaning assembly, a dust removal assembly, and a sweeping assembly. This invention effectively avoids the problem of difficulty in removing dust and impurities remaining on the stage, which can affect the stage's light transmittance. Furthermore, the cleaning process on the stage surface also removes dust from the objective lens, improving the device's practicality.

The invention discloses an inverted microscope objective lens, which includes a first lens group and a second lens group; the first lens group includes a first lens with positive refractive power, the second lens group has positive refractive power, and the second lens group includes a second lens, a third lens, a fourth lens, a fifth lens, a sixth lens, a seventh lens and an eighth lens in sequence, the second lens has positive refractive power, the third lens has negative refractive power, the fourth lens has positive refractive power, the fifth lens has positive refractive power, the sixth lens has negative refractive power, the seventh lens has positive refractive power, the eighth lens has negative refractive power, and the second lens group has at least two groups of cemented lenses. The invention includes a first lens group and a second lens group along the direction from the object side to the image side, and by designing eight lenses accordingly, the inverted objective lens optical system has the characteristics of large magnification, long working distance, large numerical aperture, high resolution performance, small chromatic aberration, large field of view, small number of lenses, and short total length of the optical system.

The invention provides a CO2-water / salt water displacement interface instability characterization system and method based on a microfluidic technology, and belongs to the field of carbon dioxide salt water layer sealing. According to the invention, a micro-fluidic chip with specific pore structure characteristics is designed, a micro-fluidic visual experiment system based on an inverted microscope is established, and characterization parameters including capillary number, viscosity ratio, fractal dimension and the like are extracted by carrying out digital processing on continuously collected image sequences; the interface evolution characteristics of CO2 displacement water / salt water are described through the capillary number and the viscosity ratio, and the interface complexity is quantitatively described by means of fractal dimensions so as to reflect the instability of the interface. According to the method, the interface instability in the displacement process is converted into a numerical value capable of being accurately predicted from traditional qualitative description, the influence mechanism of the pore structure, the viscosity ratio, the capillary tube number and the like on the interface instability can be visually revealed, and key data support is provided for CO2 geological sequestration evaluation.

The utility model relates to a separating, screening and sucking device for cells. The separating, screening and sucking device comprises a rotary sucking module, a separating and screening module, a visual detection module and a discharging and carrying module, the rotary suction module comprises a rotary manipulator module and a suction module arranged at the tail end of a mechanical arm of the rotary manipulator module; the separating and screening module comprises an objective table and an inverted microscope module arranged below the objective table; the visual detection module is used for guiding and positioning the rotary suction module and realizing displacement compensation; after the separating and screening module completes screening through vision, the rotary suction module sucks the microorganisms to the discharging and carrying module. Compared with manual cell selection, the efficiency of manual cell extraction can be improved; operations such as boring cell selection and easy errors are omitted, and the yield of manual cell selection is improved; and the device has strong universality, universality and use value in different fields and different products.

The application provides a high-speed atomic force microscope scanning method based on visual guidance, which comprises the following steps: a direct correlation model of regional gray scale variation and the end position of a scanning mechanism is established, and a magnetic hysteresis compensation strategy based on a PI inverse model is given, so that accurate servo control of a complex scanning track can be realized on the basis of low visual delay; and a device for realizing the method comprises an atomic force microscope, a flexible nanometer positioning platform, a general positioning platform, an inverted microscope, a camera, a sliding table, an inverted microscope clamp, a platform support, a computer and a visual servo control system. The atomic force microscope scanning control method and device provided by the application have the advantages of high upper limit of scanning frequency, high scanning precision and high efficiency in improving the scanning performance of an existing atomic force microscope.

To provide an inverted microscope equipped with an immersion liquid supply device capable of easily supplying immersion liquid even during observation.SOLUTION: An inverted microscope comprises: a liquid immersion objective lens 102a; a dry objective lens 102b; a rotary revolver 103 on which a plurality of objective lenses are mountable and which rotates to switch the objective lenses; a support base 100; a focus mechanism 104 which moves the rotary revolver 103 in an optical axis O3a direction with respect to the support base 100; an immersion liquid supply base 11a fixed to the support base 100; an immersion liquid supply unit 11b which includes an immersion liquid supply tube 11c that is movable in an optical axis direction with respect to the immersion liquid supply base 11a and supplies immersion liquid to the liquid immersion objective lens; and transmission mechanisms (11ba, 11a) which allow transmission of movement of the focus mechanism in the optical axis direction to the immersion liquid supply unit when the liquid immersion objective lens is arranged within a focus range on the optical axis, and releases transmission of movement of the focus mechanism in the optical axis direction to the immersion liquid supply unit when the liquid immersion objective lens is arranged in a downward region located on the optical axis and outside the focus range.SELECTED DRAWING: Figure 2

The utility model relates to the related technical field of single cell picking devices, in particular to a single cell clone picking device, which is used for picking single cells in a cell culture dish, the cell picking culture dish is positioned in a picking area of an inverted microscope objective table, and the single cell clone picking device is used for picking the single cells in the cell culture dish. Microscope collecting lenses with fixed positions are arranged above the picking area at intervals, and microscope collecting lenses are arranged above the picking area of the inverted microscope objective table at intervals; cells at the bottom of the culture dish are scraped off through the cell suction tube opening, the cells are sucked and stored in the cell suction tube by adjusting the position of the rubber plug through the suction adjusting assembly, the sucked cells can be pushed into a new culture dish containing a culture medium, and picking and transferring of single cell clones are achieved. The device is easy to assemble and disassemble, and is suitable for most inverted microscopes, and the cell suction tube opening covers single cell cloning, so that other parenchyma cells cannot be doped in the picking process, and the unicity of single cell cloning is ensured.

The invention discloses a low-density sperm braking method without a PVP medium in ICSI fertilization, and relates to the field of assisted reproduction therapy, and the method comprises the following steps: step 1, diluting a sperm suspension until the density is 0.1 * 10 < 6 > / ml, and placing the diluted sperm suspension in an in-vitro operating solution without PVP; step 2, under an inverted microscope, directly pressing and braking the tail of the sperm in the in-vitro operating fluid by using a microinjection needle, so that the tail of the sperm is folded or curled to deform; and step 3, transferring the braked sperms into operation liquid drops containing ovum to complete microinjection, and performing tail pressing operation in an isotonic operation liquid without PVP (Polyvinyl Pyrrolidone), so that the tail parts of the sperms present visible curled deformation or tail folding. The sperm motility destruction rate is 100% (residual motility is completely prevented from interfering fertilization), and the tail cell membrane rupture rate is 100% (it is ensured that activation factors in sperms are fully released).

Femtosecond pulse near-infrared Gaussian laser is adopted as a unified light source and is simultaneously used for time-sharing scanning optical tweezers capturing and two-photon fluorescence excitation, and a light path part is sequentially provided with a height adjusting assembly, a galvanometer scanning element, a beam expanding assembly and a telescope assembly. The light is reflected by a dichroscope configured on an inverted microscope platform, strong focusing is realized in a PDMS sample chamber by a high numerical aperture objective lens, bright field illumination is provided by a white light LED lamp, and a two-photon fluorescence signal is collected by a CMOS camera; a high-precision biosensing system based on a CRISPR / Cas12a system is constructed on the surface of a microsphere enriched with a detection signal output unit, quenching-recovery conversion of a fluorescence signal is realized, and two-photon fluorescence imaging is carried out in a multiple optical capture microsphere array formed by time-sharing scanning optical tweezers. The method has the advantages of high detection accuracy and sensitivity, low background signal, good imaging stability, strong anti-interference capability in a complex biological medium and the like.

An apparatus controls a series of actuators mounted onto inverted microscope assemblies to add 3D bioprinting and pick and place capabilities on a microscopic scale. One or more syringes are also mounted to the apparatus and the plunger of each syringe is independently actuated to extrude or extract fluids from the optically aligned needle. A software package communicates with the microscope x, y stage as well as the on-board motor controller which actuates the syringes and needle to set the x, y, z position and syringe in real time.

The utility model relates to observation device technical field belongs to a kind of mouse live imaging fixing device based on inverted microscope, including fixed on the fixed device box base body and base of microscope platform, the fixed device box base body bottom is fixedly connected on base, the upper portion of fixed device box base body is equipped with detachable seal cover, the base is opened on the observation window, the fixed device box base body perimeter is also opened experimental fixed hole. The utility model can flexibly match the tissue of surgical exposure mouse and the mouse of surgical installation observation window are observed using inverted microscope, reduce the development cost of multiple sets of fixed device, can also achieve certain mouse physiological state maintenance function, and compatible various equipment required by experiment, simplify live imaging observation procedure.

This invention discloses an illumination device for solving the concave liquid surface effect in well plate imaging, belonging to the field of microscope light source technology. The device includes an inverted microscope illumination source and several annular light sources distributed in a dome-like pattern around it. The inverted microscope illumination source is composed of a central light source, a collimating lens, and a condenser lens in sequence. The annular light sources contain multiple LED beads, and the size of the rings matches the diameter of the microwells in a common cell culture plate, with adjustable angles. Through the synergistic effect of traditional vertical illumination and dome-shaped oblique illumination, this invention effectively improves the imaging quality of well plates, adapts to microwell plates of different diameters, is easy to operate, and highly practical, providing strong support for cell observation and other related work.

The utility model belongs to the technical field of scanning ionic conductance microscopes, and discloses a scanning ionic conductance microscope platform for single cell scanning and detection, which comprises an inverted microscope body, a culture dish tray of the inverted microscope body is detachably connected with a protection device, and the protection device is internally provided with an accommodating cavity for embedding a culture dish. The containing cavity extends to the bottom of the protection device, a first observation hole communicated with the top of the containing cavity is formed in the top of the protection device, a transparent cover plate is arranged on the upper side of the first observation hole, and a second observation hole is formed in the transparent cover plate; a carbon dioxide concentration detection device is arranged in the containing cavity, a carbon dioxide input hole and a carbon dioxide concentration detection device wire passing hole are further formed in the protection device, and the protection device is connected with a carbon dioxide supply device through the carbon dioxide input hole. According to the scanning ion conductance microscope platform, on the basis of an existing scanning ion conductance microscope platform, the gas environment of cells during in-vitro culture can be guaranteed, and meanwhile target cells can be conveniently found.

This invention relates to the field of fecal testing, and more specifically, to a method for detecting human parasitic pathogens without the use of slides and coverslips, comprising the following steps: Step 1, adding an appropriate amount of water to the culture wells of a cell culture plate, placing an appropriate amount of feces in the water of the culture well and stirring evenly; Step 2, cutting a piece of textile or nylon mesh of similar size to the culture well and placing it into the culture well to isolate the fecal residue and fecal liquid from each other; Step 3, after stirring and mixing the fecal liquid, transferring the fecal liquid to the culture wells of a new cell culture plate, covering it with the cell plate cover, and examining it under a microscope using an inverted microscope.

1. The name of this design product: Neuroelectrophysiological detection and stimulation platform. 2. Purpose of this product design: This product design is used for neuroelectrophysiological detection and stimulation of isolated cells and can be used in conjunction with an inverted microscope. 3. The key point of the design of this product lies in its shape. 4. The picture or photo that best illustrates the design points: Stereoscopic drawing 1.

The invention discloses an aluminum-plastic interface in-situ morphology and spectrum combined analysis under a hydrothermal environment, which comprises a sample holder, an in-situ heating table is arranged on the sample holder, a hydrothermal reactor for placing a sample is arranged in the in-situ heating table, observation windows are arranged on the upper side and the lower side of the in-situ heating table, and the in-situ heating table is connected with the hydrothermal reactor. An inverted microscope is arranged below an observation window on the lower side of the in-situ heating table, a camera is arranged at an observation port of the inverted microscope, and a Fourier infrared ATR spectrometer and a Raman spectrometer are arranged above the observation window on the upper side of the in-situ heating table; the camera, the Fourier infrared ATR spectrometer and the Raman spectrometer are respectively connected with the server, and the server is sequentially connected with the machine learning module and the visual report module. The aluminum-plastic interface in-situ morphology and spectrum combined analysis system under the hydrothermal environment has the problems of insufficient real-time monitoring and incomplete data analysis in the current research.

1. Name of the Design Product: Inverted Microscope (PX55). 2. Use of the Design Product: An instrument for magnifying tiny objects. 3. Design Key Points of the Design Product: Lies in the shape. 4. Picture or Photograph that Best Illustrates the Design Key Points: Perspective view.

The utility model relates to the technical field of cell culture consumables, in particular to a dynamic stretching cell culture dish capable of real-time microscope observation and marker detection, which comprises a culture dish main body, a bottom membrane and a sensor, the bottom film covers the bottom surface of the culture dish main body; the culture dish main body can be stretched along a set direction, and the upper surface of the culture dish main body is provided with a culture pond penetrating through the upper surface and the lower surface; among the side walls around the culture pond, the side wall parallel to the stretching direction is a stretching side wall, and the side wall perpendicular to the stretching direction is a fixed side wall; two groups of clamping plates are symmetrically arranged in the culture pond, each group of clamping plates is arranged close to the fixed side wall, and a set distance is formed between each group of clamping plates and the fixed side wall; and the sensors are arranged between each group of clamping plates and the fixed side wall. Stretching along a set direction to implement mechanical stimulation on the cells; the bottom membrane with limited thickness is matched with the inverted microscope, so that the change condition of the cells after mechanical stimulation can be observed; the sensor can detect cell secretion factors in real time.

The utility model discloses an inverted microscope multicolor fluorescence illumination device, including microscope body, the front end of microscope body is provided with the focusing knob, the top left side of microscope body is connected with the observation cylinder, the top of observation cylinder is installed with the ocular lens, the top middle section of microscope body is equipped with the accommodation groove, the upper end of accommodation groove is connected with the object table, the inner chamber bottom of accommodation groove is installed with the objective lens, the lower extreme of accommodation groove is provided with the fluorescence filter component, the top right side of microscope body is connected with the stand, the cross end of stand is connected with the light collector, the right end surface bottom of microscope body is connected with T type rail, the outside of T type rail is equipped with the supporting plate, the top of supporting plate is connected with the illumination box, the convenience of multicolor fluorescence observation is greatly promoted, is favorable for improving the imaging quality of fluorescence image to obtain clearer, reliable observation result.

The utility model relates to the technical field of microscope loading platforms, in particular to an inverted microscope dissecting table which comprises a main sliding table, a control mechanism and a plurality of sets of driving mechanisms. The control mechanism comprises a shell and a mounting seat fixed in the shell, the mounting seat is movably connected with a control rod, the control rod can transversely and longitudinally swing on the mounting seat, and a plurality of pressing arms are fixed on the control rod; a plurality of inching switches fixed at the bottom of the shell are arranged below the pressing arm; the driving mechanism comprises a hydraulic pump, a hydraulic cylinder and a communicating and oil inlet pipeline, and an electromagnetic valve is mounted on the oil inlet pipeline and connected with an inching switch in series; the end of a piston rod of the hydraulic cylinder is detachably connected with a transmission cavity and a hydraulic pipe which are communicated with each other, one end of the hydraulic pipe is communicated with the main sliding table, a piston head is slidably connected into the transmission cavity, and the end of the piston rod is fixed to the piston head. According to the device, the microscope objective table can be finely and stably adjusted, so that the convenience in the dissecting operation process is improved.

An automatic oil dispenser (10) for mounting onto an objective (14) in an inverted microscope system, the dispenser comprising: an outer annulus (52) configured to contact the objective to form a cavity; an inlet port (20) below the outer annulus (52) for inputting oil into the outer annulus (52); a pump for driving oil upwards through and out of the dispenser; an inner annulus (56) which tapers to an annular outlet (36); and a channel (53) connecting the two annuli such that oil fills the outer annulus (56), then the inner annulus (52) and then erupts through the outlet (36) onto an objective lens (26).

An inverted microscope is provided, the inverted microscope including a microscope stage which includes an opening prepared for transmitted light illumination and which is designed to receive a sample holder and an imaging optical unit arranged below the microscope stage. A closed lower incubation space which surrounds at least the imaging optical unit is arranged adjacent to a lower side of the microscope stage. The inverted microscope is configured such that a temperature in the lower incubation space is adjustable to a specifiable target temperature, for the purposes of which at least one temperature sensor is arranged in the lower incubation space, the measurement signal of the at least one temperature sensor serving to set the specifiable target temperature.

The invention discloses a multichannel fluorescence imaging system based on time sequence pulse excitation and asynchronous acquisition, and particularly relates to the technical field of biomedical engineering and optical microscopic imaging. Comprising an inverted microscope main body, a multi-wavelength laser excitation module, a high-speed Scmos imaging device supporting hardware triggering, a lower computer time sequence control unit based on a microcontroller, and an upper computer calculation processing platform. The lower computer utilizes a hardware timer to generate a synchronous control signal with microsecond-level precision, adopts a time division multiplexing strategy to control opening of lasers with different wavelengths, and is matched with hardware handshake logic to force the camera to perform global shutter synchronous exposure. The upper computer builds a memory asynchronous buffer architecture, decouples high-bandwidth image acquisition and a low-speed hard disk storage process by using a producer-consumer model, and temporarily stores original data by using a memory buffer pool.