The invention belongs to the technical field of
biology and particularly relates to a high-
throughput screening
system and method of fast growing microalgae strains. The
system comprises a
liquid drop monodisperse single
cell wrapping unit and a reinjecting screening unit. The method comprises steps as follows: microalgae cells are washed and dispersed in gel to proper concentration and injected into a micro-fluidic
chip to form monodisperse single
cell liquid drops, and microspheres wrapped in an
oil phase are subjected to deoiling,
filtration and redispersion and cultured in a constant-temperature
incubator; whether living cells exist and the growing speed of the cells are judged according to imaging by the aid of an
inverted microscope, gel amplification pictures are collected through
random interval sampling, gel microspheres where the cells grow are counted, the growth condition of microalgae in the single
cell level is further monitored, and the microalgae strains with remarkable growth
rate difference are separated on the basis. According to the constructed high-
throughput screening
system and method of the fast growing microalgae strains, the system is simple, the cost is low, and the system and the method can be used for screening of phenotypic correlation of growth of a
mutant library of alga.