High-throughput screening system and method of fast growing microalgae strains

Abstract

The invention belongs to the technical field of biology and particularly relates to a high-throughput screening system and method of fast growing microalgae strains. The system comprises a liquid drop monodisperse single cell wrapping unit and a reinjecting screening unit. The method comprises steps as follows: microalgae cells are washed and dispersed in gel to proper concentration and injected into a micro-fluidic chip to form monodisperse single cell liquid drops, and microspheres wrapped in an oil phase are subjected to deoiling, filtration and redispersion and cultured in a constant-temperature incubator; whether living cells exist and the growing speed of the cells are judged according to imaging by the aid of an inverted microscope, gel amplification pictures are collected through random interval sampling, gel microspheres where the cells grow are counted, the growth condition of microalgae in the single cell level is further monitored, and the microalgae strains with remarkable growth rate difference are separated on the basis. According to the constructed high-throughput screening system and method of the fast growing microalgae strains, the system is simple, the cost is low, and the system and the method can be used for screening of phenotypic correlation of growth of a mutant library of alga.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a high-throughput screening system and method for rapidly growing microalgae strains at the single-cell level based on the droplet microfluidic technology in the gel droplet microfluidic technology. Background technique [0002] Microalgae is a kind of ancient low-level plants, which are widely distributed in oceans, freshwater lakes and other waters. They can directly use sunlight, carbon dioxide and simple nutrients containing nitrogen, phosphorus and other elements to grow rapidly. Microalgae cells contain a variety of high-value nutrients and chemical raw materials, polysaccharides, proteins, pigments, etc. produced by cell metabolism. They are used in the pharmaceutical industry, food industry, animal feed, environmental testing and purification, biotechnology and renewable energy manufacturing. There are wide applications. Algae cultivation is a production process o...

AI Technical Summary

Problems solved by technology

[0003] Traditional screening methods mostly use sequencing, PCR, or methods with specific response mechanisms to screen algal strains, which is time-consuming and laborious. It can neither obtain single cells efficiently and quickly, nor perform accurate high-throughput separation of algal strains with specific phenotypes.

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