77 results about "Cell encapsulation" patented technology

A new class of poly(beta-amino alcohols) (PBAAs) has been prepared using combinatorial polymerization. The inventive PBAAs may be used in biotechnology and biomedical applications as coatings (such as coatings of films or multilayer films for medical devices or implants), additives, materials, excipients, non-biofouling agents, micropatterning agents, and cellular encapsulation agents. When used as surface coatings, these PBAAs elicited different levels of inflammation, both in vitro and in vivo, depending on their chemical structures. The large chemical diversity of this class of materials allowed us to identify polymer coatings that inhibit macrophage activation in vitro. Furthermore, these coatings reduce the recruitment of inflammatory cells, and reduce fibrosis, following the subcutaneous implantation of carboxylated polystyrene microparticles. These polymers may be used to form polyelectrolyte complex capsules for cell encapsulation. The invention may also have many other biological applications such as antimicrobial coatings, DNA or siRNA delivery, and stem cell tissue engineering.

The present invention provides a synthetic, poorly crystalline apatite (PCA) calcium phosphate containing a biologically active agent and / or cells (preferably tissue-forming or tissue-degrading cells). The compositions provided by the present invention are useful for a variety of in vivo and in vitro applications, including drug delivery (for example, to bony sites, the central nervous system, intramuscular sites, subcutaneous sites, interperitoneal sites, and occular sites) tissue growth (preferably bone or cartilage) osseous augmentation, and methods of diagnosing disease states by assaying tissue forming potential of cells isolated from a host. The invention also provides methods of preparing delivery vehicles, of altering delivery vehicle characteristics, and of delivering biologically active agents to a site. The invention further provides in vitro cell culture systems and cell encapsulation materials. The invention is useful for both medical and veterinary applications.

A multi-layered microcapsule has an inner extracellular matrix and an outer shell. The inner extracellular matrix includes a first inner layer of biopolymer and a second intermediate layer of polymer that provides partial immune-protection and holds the first layer in place. The outer shell can form an exoskeleton to provide mechanical stability. Each of the individual layers can be varied to optimize mechanical stability, cell function, and immuno-protection.

Methods and devices for the formation of droplets of a first fluid in a second fluid and the encapsulation of particles or cells within such droplets are disclosed. Impetus for droplet formation is provided by the creation of a transient bubble, which may be induced using a pulsed laser. Droplet volume and the frequency at which droplets are formed can be controlled by modulation of the pulsed laser. The disclosed methods and devices are particularly suitable for use in microfluidic devices.

The present inventions in various aspects provide elastic polymers compositions for encapsulation of cells. In various embodiments, the polymers are formed by the reaction of a multifunctional alcohol or ether and a difunctional or higher order acid to form a pre-polymer, which is cross-linked in the presence of glycerol and a population of cells to form elastic porous polymer scaffolds suitable for cell encapsulation and / or proliferation.

Covalently modified alginate polymers, possessing enhanced biocompatibility and tailored physiochemical properties, as well as methods of making and use thereof, are disclosed herein. The covalently modified alginates are useful as a matrix for the encapsulation and transplantation of cells. Also disclosed are high throughput methods for the characterizing the biocompatibility and physiochemical properties of modified alginate polymers.

Disclosed herein are vascular cell encapsulation devices that can deliver cell type(s) for the treatment of a disease or disorder.

A method for encapsulating photovoltaic cells into single functional units is described. These units share the mechanical and electric properties of the encapsulation layers and allow for flexible module architecture to be implemented at the cell level. This enables cost reduction and improved performance of photovoltaic power generation.

A new class of poly(beta-amino alcohols) (PBAAs) has been prepared using combinatorial polymerization. The inventive PBAAs may be used in biotechnology and biomedical applications as coatings (such as coatings of films or multilayer films for medical devices or implants), additives, materials, excipients, non-biofouling agents, micropatterning agents, and cellular encapsulation agents. When used as surface coatings, these PBAAs elicited different levels of inflammation, both in vitro and in vivo, depending on their chemical structures. The large chemical diversity of this class of materials allowed us to identify polymer coatings that inhibit macrophage activation in vitro. Furthermore, these coatings reduce the recruitment of inflammatory cells, and reduce fibrosis, following the subcutaneous implantation of carboxylated polystyrene microparticles. These polymers may be used to form polyelectrolyte complex capsules for cell encapsulation. The invention may also have many other biological applications such as antimicrobial coatings, DNA or siRNA delivery, and stem cell tissue engineering.

The invention discloses preparation and application of a pH glucose dual sensitive hydrogel. The hydrogel is formed by crosslinking of phenylboronic acid modified chitosan and an active functional group equipped crosslinking agent through imine bond and phenylboronic acid ester two dynamic covalent bonds, and has injectability and self-repairing performance. The imine bond is stable under normal physiological pH value, can hydrolyze under slightly acidic pH value, and is sensitive to the pH value change. Phenylboronic acid ester can undergo competing reaction in the presence of glucose, so that hydrogel can have network structure change so as to have glucose responsiveness. By regulating the structure, composition and functional groups of phenylboronic acid modified chitosan and the crosslinking agent, the rheological properties of the hydrogel can be adjusted, and activity release of the hydrogel to a substrate can be controlled. The system can be used as a drug carrier to convey various antitumor and diabetes drugs, also can be applied as a tissue engineering scaffold material to cell encapsulation and culture, thus realizing synergic treatment on the drug and cell level.

The invention relates to a method for encapsulating living cells and labels, as well as encapsulated labelled cells and kits for performing such encapsulation. The encapsulated cells may be useful in multiple parallel tissue culture experiments, where the labels in each microcapsule may be used to decipher a cells path through a series of culturing steps.

Zwitterionic polymers or biocompatible polymers with improved properties for cell encapsulation, coating of devices, or a combination thereof are described. The biocompatible polymer contains a zwitterionic monomer, a monomer with a reactive side chain, and optionally another hydrophobic monomer or a neutral hydrophilic monomer. The zwitterionic polymers are cross-linked with a cross-linker via covalent bond to form a zwitterionic hydrogel in the presence of cells. Also provided, are methods of making and using the zwitterionic polymers.

The present invention provides novel, biocompatible matrices for cell encapsulation and transplantation. It further provides methods for delivering agents to encapsulated cells and to the local environment of a host system. The invention also provides methods for targeting and manipulating particular cells and / or proteins of the host system. In a composition aspect of the invention, a composition including a collection of capsules is provided. The capsules comprise an inner core, and the inner core is covered by an outer shell composed of a positive polyelectrolyte and a negative polyelectrolyte. The inner core of the capsules contains at least one cell.

Provided herein is a cell encapsulation device comprising an internal chamber suitable for holding biological agents, wherein the internal chamber is disposed between a first semipermeable layer and a second semi-permeable layer, and the first and second semi-permeable layers are mounted on a supporting frame surrounding a perimeter of the internal chamber; wherein the supporting frame comprises a first frame element and a second frame element, and the first and second frame elements co-operate to position the first and second semipermeable layers at a predetermined separation distance between the layers.

The invention relates to the field of responding type injectable hydrogels, in particular to a dipeptide hydrogel, a preparation method and application thereof. A dipeptide molecule is of an XY structure, wherein X is selected from tyrosine, phenylalanine, tryptophan or histidine; Y is selected from aspartic acid, asparagine, glutamic acid or glutamine. The hydrogel has a quick ultrasonic response characteristic, can be quickly recovered to a gel state after stimulating response, is stable and uniform in properties, and can be applied to the fields of biological nano-materials, medicament controlled-release, medicament delivery, cell encapsulation and tissue repair.

The invention relates to the technical field of biomedical polymer materials, in particular to injectable thermosensitive physical hydrogel with functional side groups and a preparation method thereof. Polyethylene glycol serves as a hydrophilic block (B), polycaprolactone serves as a hydrophobic block (A), a polymer composition with the functional side groups is connected, an aqueous solution, with a certain mass percentage, of the composition is in a free flowing sol state when the temperature of the aqueous solution is lower than that of a human body and is in a non-flowing gel state when the temperature of the aqueous solution is equal to that of the human body, and conversion from sol to gel is achieved when the low temperature is raised to the high temperature. The hydrogel is injectable and biodegradable, has good biocompatibility and can be widely applied to medicine conveying, cell encapsulation and tissue repair.

Covalently modified alginate polymers, possessing enhanced biocompatibility and tailored physiochemical properties, as well as methods of making and use thereof, are disclosed herein. The covalently modified alginates are useful as a matrix for the encapsulation and transplantation of cells. Also disclosed are high throughput methods for the characterizing the biocompatibility and physiochemical properties of modified alginate polymers.

A two-component, molecular-recognition gelation strategy that enables cell encapsulation without the need for environmental triggers is provided. The two components, which in one example contain WW and polyproline-rich peptide domains that interact via hydrogen bonds, undergo a sol-gel phase transition upon simple mixing. Hence, physical gelation is induced by the mixing of two components at constant environmental conditions, analogous to the formation of chemically crosslinked epoxies by the mixing of two components. Variations in the molecular-level design of the two components are used to predictably tune the association energy and hydrogel viscoelasticity. These hetero-assembly physical hydrogels encapsulate neural progenitor cells at constant physiological conditions within 10 seconds to create uniform 3D cell suspensions that continue to proliferate, differentiate, and adopt well-spread morphologies.

Sealing cell for encapsulating a microelectronic component arranged on a substrate, with a cap, said sealing cell including: a bottom including a receiving zone for the substrate and a peripheral zone surrounding the receiving zone, a side wall formed of an internal face, an external face and an upper face, the upper face being configured to support the cap facing the receiving zone, an opening, arranged in the bottom of the cell, in the side wall, or in the cap, the opening being configured to be connected to a pumping system, in such a way as to be able to place under controlled atmosphere a cavity delimited by the side wall, the bottom and the cap.

Methods and devices for the formation of droplets of a first fluid in a second fluid and the encapsulation of particles or cells within such droplets are disclosed. Impetus for droplet formation is provided by the creation of a transient bubble, which may be induced using a pulsed laser. Droplet volume and the frequency at which droplets are formed can be controlled by modulation of the pulsed laser. The disclosed methods and devices are particularly suitable for use in microfluidic devices.

The present invention provides novel, biocompatible matrices for cell encapsulation and transplantation. If further provides methods for delivering agents to encapsulate cells and to the local environment of a host system. The invention also provides methods for targeting and manipulating particular cells and / or proteins of the host system. In a composition aspect of the invention, a composition including a collection of capsules is provided. The capsules comprise an inner core, and the inner core is covered by an outer shell composed of a positive polyelectrolyte and a negative polyelectrolyte. The inner core of the capsules contains at least one cell.

Positively-charged initiator polymers having a polymerization initiator group and a cationic portion are provided. The initiator polymers can be used with a polymerizable material for the formation of a polymeric matrix on a surface. The initiator polymers are particularly useful for cell encapsulation using macromers.

A method for encapsulating photovoltaic cells into single functional units is described. These units share the mechanical and electric properties of the encapsulation layers and allow for flexible module architecture to be implemented at the cell level. This enables cost reduction and improved performance of photovoltaic power generation.

The present disclosure provides light-sensitive protein hydrogels, and methods of their use thereof. The hydrogels can be used for cell encapsulation, culturing, and selective release under appropriate light conditions.

The invention relates to the field of new energy source lithium battery production, in particular to a square lithium battery cell encapsulation aluminum shell device. The square lithium battery cellencapsulation aluminum shell device comprises a machine frame assembly, a second work table, an aluminum shell feeding device and an aluminum shell carrying device, wherein the second work table is fixedly arranged on the machine frame assembly; a work procedure for completing the battery cell and aluminum shell compression is completed in the second work table; the aluminum shell feeding device is fixedly arranged on the machine frame assembly, is arranged beside the second work table and is used for conveying an aluminum shell to be processed; the aluminum shell carrying device is fixedly arranged on the second work table and can leftwards and rightwards move; the aluminum shell on the aluminum shell feeding device is carried on the second work table for processing. The square lithium battery cell encapsulation aluminum shell device has the advantages that the operation of charging a battery cell into the aluminum shell is completed; the automation level is improved; the square lithium battery production is more intelligentized.

An interfacial technique utilizes hydrodynamic micro-vortices to perform (i) high efficiency single cell encapsulation and (ii) size-selective capturing of cells based on their sizes in a single microfluidic device. A notable feature of this technique is that it can perform high efficiency single cell encapsulation at low cell concentrations, and this technique is all passive, controlled only by the flow rates of the two phases and does not require complex structures or on-chip active devices. Single bead / cell encapsulation was demonstrated at 50% efficiency, which is at least 10 times greater than the random encapsulations at the introduced cell concentrations. Also demonstrated is the selective trapping of cells based on their sizes. This present technique expands the capabilities of droplet microfluidics for applications ranging from single cell genomics, proteomic assays to sample preparation.

The invention relates to the technical field of solar battery cell encapsulation, particularly to an ejector pin structure of a laminating machine. The laminating machine comprises a heating plate, wherein a guide hole is formed in the heating plate; the upper surface of the heating plate is a plane. The ejector pin structure comprises an ejector pin body matched with the guide hole and a platform covering the top of the ejector pin body, wherein the upper surface the platform is a plane; the ejector pin body is inserted in the guide hole; a sunken part matched with the platform is arranged at the part, right below the platform, of the heating plate; when the lower surface of the platform is in contact with the bottom surface of the sunken part, the upper surface of the platform and the upper surface of the heating plate are level. According to the ejector pin structure of the laminating machine provided by the invention, the materials of the ejector pin body, the platform and the heating plate are the same, so that consistency of heat transfer among the ejector pin part and other parts of the heating plate is guaranteed when the heating plate is heated; the platform is added at the top of the ejector pin body, so that pieces to be subjected to lamination are uniformly heated when the laminating machine is heated, consistency of EVA crosslinking degree in the pieces to be subjected to lamination is guaranteed, and the properties of a solar component are guaranteed.

A two-component, molecular-recognition gelation strategy that enables cell encapsulation without the need for environmental triggers is provided. The two components, which in one example contain WW and polyproline-rich peptide domains that interact via hydrogen bonds, undergo a sol-gel phase transition upon simple mixing. Hence, physical gelation is induced by the mixing of two components at constant environmental conditions, analogous to the formation of chemically crosslinked epoxies by the mixing of two components. Variations in the molecular-level design of the two components are used to predictably tune the association energy and hydrogel viscoelasticity. These hetero-assembly physical hydrogels encapsulate neural progenitor cells at constant physiological conditions within 10 seconds to create uniform 3D cell suspensions that continue to proliferate, differentiate, and adopt well-spread morphologies.