28 results about "Cell encapsulation" patented technology

The invention belongs to the technical field of biology, and particularly relates to a high-throughput preparation method of a single-cell-derived organoid based on a microcapsule spherical shell structure, and the preparation method comprises the following steps: preparing an aqueous two-phase microcapsule encapsulated with a single cell, culturing the single cell in the aqueous two-phase microcapsule to obtain the organoid, the substances of the inner phase and / or the outer phase respectively comprise any one or a combination of more of the following substances: methacrylated gelatin, carboxymethyl cellulose, glucan, sodium alginate and polyvinyl alcohol. The invention also provides the organoid prepared by the preparation method and application of the preparation method. According to the invention, the microcapsule with a permeable core-shell structure is formed by packaging the microcapsule in a single-cell form in two-aqueous-phase liquid drops, the wrapping rate is effectively controlled and adjusted on the premise of conforming to Poisson distribution by optimizing parameters, and the single-cell wrapping rate and the organoid single-cell induction efficiency are optimized.

The invention relates to a microbial single-cell gelation encapsulation method based on aloe gel and application, the method realizes single-cell gelation encapsulation through electrostatic interaction of sterile aloe gel and intestinal anaerobic bacteria (such as Agathobacter rectasis), and the method comprises the specific steps of aloe gel preparation, bacterial suspension culture and oscillation mixing to form a stable structure. The preparation method has the advantages that the acid resistance and biocompatibility of the natural aloe gel are utilized to replace a traditional synthetic material, so that the gastrointestinal tolerance of thalli is improved; meanwhile, the synergistic effect of aloe active ingredients (such as aloe-emodin) and intestinal bacterial metabolites (butyric acid) is combined, so that the anti-inflammatory and intestinal steady-state regulation and control effects are enhanced. Experiments show that the survival rate of the encapsulated bacteria in simulated gastric juice within 4 hours is larger than or equal to 90%, the intestinal colonization rate is effectively increased compared with naked bacteria, the inflammatory factor level of a colitis model mouse can be remarkably reduced, and a safe and efficient new strategy is provided for micro-ecological treatment of inflammatory bowel diseases.

The present disclosure relates to a modified polymer and a use thereof. Specifically, the present disclosure provides a modified monomer as shown in formula I, a polymer containing same, a hydrogel, a capsule, and a corresponding use. An alginate of the present disclosure is used for cell encapsulation, and can provide an anti-fibrotic effect and reduce foreign body reaction.

An implantable therapeutic delivery device comprising a porous pouch and a cell encapsulation device configured to be housed in the porous pouch. The porous pouch can comprise a bioabsorbable material and / or a vascularization promoter, and the porous pouch can be packaged separately from the cell encapsulation device.

The invention relates to hydrogel with ice recrystallization inhibiting activity, a preparation method of the hydrogel and application of the hydrogel in cell cryopreservation. Firstly, novel hydrogel is provided; a polysaccharide-PVA graft and sulfhydrylation modified gelatin are mixed to prepare the hydrogel with ice recrystallization inhibiting activity, in-situ cell encapsulation culture verifies that the hydrogel has good cell compatibility and can maintain the proliferation performance of cells, and the hydrogel is suitable for cryopreservation of a cell-hydrogel structure after long-term culture and has good application prospects. And the biological activity of the cells is maintained.

A fish liver decellularized extracellular matrix based microfluidic 3D printing hydrogel for liver regeneration, and a preparation method thereof are provided. A fish liver decellularized extracellular matrix (dECM) is combined with gelatin methacryloyl (GelMA), and loaded with hepatic spheroids derived from induced pluripotent stem cells (iPSC-hep) for liver regeneration. The fish liver decellularized extracellular matrix based microfluidic 3D printing hydrogel of the present disclosure has excellent biocompatibility and retains intact endogenous growth factors, maintains the biological activity of cells, ensures effective cell encapsulation, and is conducive to robust functional expression of iPSC-hep. After being transplanted in vivo, the hydrogel significantly improves the survival rate and liver function of mice with acute liver failure, and promotes liver regeneration and repair.

A cell encapsulation device for implantation in a body includes one or more cell encapsulation layers, each of the one or more cell encapsulation layers including at least one membrane and a guide tube. The at least one membrane is semi-permeable. The at least one membrane forms a chamber for encapsulating cells and at least one entry port through the at least one membrane. The guide tube extends from the at least one entry port into the chamber. The guide tube includes a porous wall along at least a portion of its length. The guide tube is capable of guiding movement of a catheter inside the chamber.

Methods and apparatus for acoustically assisted droplet generation with or without cell encapsulation.

The invention provides a cell packaging oxygen production device and application thereof, and belongs to the technical field of implantable medical instruments, the cell packaging oxygen production device comprises: a raw material storage module for storing a hydrogen peroxide solution; the diffusion control module is arranged between the raw material storage module and the cell packaging module and comprises a catalytic cavity and an isolation structure, and a catalyst is arranged in the catalytic cavity and used for catalyzing hydrogen peroxide molecules to generate oxygen; the isolation structure comprises a first isolation membrane and a second isolation membrane, and the first isolation membrane is arranged between the raw material storage module and the catalytic cavity and controls hydrogen peroxide molecules to diffuse into the catalytic cavity; the second isolating membrane is arranged between the catalytic cavity and the cell packaging module and is used for controlling oxygen to diffuse into the cell packaging module; the cell encapsulation module contains a cell encapsulation matrix containing cells and is used for receiving oxygen and supplying oxygen to the cells. The cell packaging oxygen production device can control the oxygen production rate and match the oxygen consumption demand of cells, effectively avoids the toxicity of hydrogen peroxide and the migration risk of a catalyst, and is excellent in biological safety.

A soft package lithium ion battery comprises an outer bag formed by folding a soft package film, and the outer bag comprises a battery cell packaging area located in the middle and used for containing a soft package battery cell, a first air bag located on the left side of the battery cell packaging area and a second air bag located on the right side of the battery cell packaging area. The tops of the first air bag and the second air bag are packaged by a top sealing area in a heat sealing mode, the left side of the first air bag is packaged by a first side sealing area in a heat sealing mode, the right side of the second air bag is packaged by a second side sealing area in a heat sealing mode, and an isolation pre-sealing line parallel to the second side sealing area is arranged on the left side of the second air bag and close to the battery cell containing area. The isolation pre-sealing line is formed by gap heat sealing, the isolation pre-sealing line comprises an edge sealing area composed of a plurality of sealing edges which are sequentially arranged at intervals, and an unsealed area is formed between every two adjacent edge sealing points. According to the utility model, the extraction amounts of electrolyte and gas on the two sides of the soft package battery cell are almost the same in the secondary sealing process, so that the hardness of the two sides of the battery cell is consistent, and the internal consistency of the battery cell is better.

Thin film devices, e.g., multilayer thin film devices, that encapsulate cells for transplantation into a subject are provided. Also provided are methods of using and methods of preparing the subject devices. The thin film devices include a first porous polymer layer and a second porous polymer layer that define a lumen therebetween and encapsulate a population of cells within the lumen. The thin film devices can promote vascularization into the lumen of the device via the pores in the first polymer layer and / or second polymer layer; limit foreign body response to the device; limit ingress of cells, immunoglobulins, and cytokines into the lumen via the first and the second polymer layers; and release from the first polymer layer and / or the second polymer layer molecules secreted by the population of cells.

The invention relates to a cell encapsulation system (1), the system comprising at least: a first container (11) for holding a cell solution and a second container (12) for holding a gelling solution, a milli-fluidic or micro-fluidic encapsulation device (20) connected to the containers to form cell microcompartments, the outer layer of which is the gelling solution and the core the cell solution, wherein each container is connected to an inlet of the encapsulation device by a distributor (31, 32) associated with a solution displacement system (41, 42) capable of causing the solution contained in said container (11, 12) to flow from said distributor to the inlet of the encapsulation device (20), characterized in that the first distributor (31) has at least one upward-extending portion. Figure to be published with the abstract: Fig. 1

The invention relates to a method for dynamically encapsulating microbial cells by using a hydrogen bond organic framework material, and belongs to the technical field of cell encapsulation. The method comprises the following steps: preparing nano HOF, dispersing cells in a culture medium, adding a nano HOF aqueous dispersion, culturing, and dynamically packaging proliferative cells by the HOF. According to the invention, a hydrogen bond organic framework material monomer containing carboxyl is selected to encapsulate cells, self-assembly of organic molecule units on the surfaces of the cells is realized through a dynamic hydrogen bond network, and highly reversible hydrogen bonds interact to construct an intelligent encapsulation shell with a controllable disassembly / assembly function; and continuous packaging protection in the whole cell proliferation process can be realized.

This invention provides a photovoltaic cell encapsulation structure and preparation method for improving weather resistance and heat dissipation efficiency. The photovoltaic cell encapsulation structure includes at least: a photovoltaic cell layer; a first encapsulation layer disposed on the light-receiving side of the photovoltaic cell layer, the first encapsulation layer containing a chemically bonded compound of organosilicon resin and nano-silica; and a second encapsulation layer disposed on the backlight side of the photovoltaic cell layer, the second encapsulation layer containing a blend of a polymer matrix and a thermally conductive filler. This invention achieves significant results in extending module lifespan, improving heat dissipation performance, and maintaining process compatibility through a dual-layer functional design of "weather resistance on top and thermal conductivity on the bottom."

The invention relates to a cell encapsulation system (1) comprising: a container (11) of a cell solution, a second container (12) of a solution suitable for gelling, an encapsulation device (20) for forming cell microcompartments, in which the containers are connected to the encapsulation device by distributors (31, 32) associated with solution displacement systems (41, 42); and comprising a control unit (4) arranged to, prior to a production step: in a priming step (E32) of the first distributor, control the displacement system (41) to circulate a portion of the cell solution in this distributor (31); in a priming step (E33) of the second distributor subsequent to the priming step (E32), control the displacement system (42) to circulate a portion of the solution suitable for gelling in this distributor (32). Figure to be published with the abbreviation: Fig. 1

The invention relates to a preparation method of a fluorescent probe for detecting the viscosity of a cell membrane in three-dimensional hydrogel, the preparation method comprises the following steps: synthesizing an intermediate Br-BODIPY, reacting the Br-BODIPY with N-methyldiallylamine in toluene, precipitating and purifying to obtain a target product fluorescent probe BODIPY-C. The fluorescent probe BODIPY-C has a viscosity sensitive rotor BODIPY and a photopolymerizable vinyl group, and can be used for detecting the viscosity of the cell membrane in the three-dimensional hydrogel. Covalent grafting to photo-crosslinkable hydrogel can be promoted through ultraviolet-initiated polymerization; the invention further discloses a fluorescent probe application method, after BODIPY-C and HM hydrogel are subjected to an ultraviolet light crosslinking reaction, BODIPY-C is combined with an HM hydrogel network in a covalent grafting mode, when the BODIPY-C is applied to a three-dimensional hydrogel system for encapsulating cells, in a fluorescent probe grafted hydrogel cell encapsulation construction body, the fluorescent probe BODIPY-C is positioned on a cell membrane in the construction body, and in the three-dimensional hydrogel system for encapsulating the cells, the fluorescent probe BODIPY-C is positioned on a cell membrane in the construction body. The change of the cell membrane viscosity can be monitored in real time through a BODIPY-C fluorescence signal, and the method is good in detection effect, can reflect the real condition of a three-dimensional culture system, and can be applied to the field of fluorescence probe detection.

The utility model provides a perovskite cell packaging assembly, which comprises a glass substrate, a perovskite cell sheet, a conductive adhesive tape, a bus bar, a packaging film and a glass cover plate from bottom to top in sequence, the glass substrate is divided into a central area and a peripheral packaging area, the perovskite cell sheet is located in the central area of the glass substrate, and the conductive adhesive tape is located in the peripheral packaging area of the glass substrate. The two conductive adhesive tapes are respectively arranged at two sides of the perovskite cell piece, the length of the conductive adhesive tapes is greater than that of the perovskite cell piece, the two bus bars are respectively overlapped and connected with the conductive adhesive tapes through welding, and the overlapping areas of the bus bars and the conductive adhesive tapes are located in the packaging area of the glass substrate. According to the perovskite cell packaging assembly, the original assembly structure is optimized, and the outdoor stability and the photoelectric conversion efficiency of the perovskite photovoltaic assembly are improved based on the welding superposition structure of the bus bar and the conductive adhesive tape.

The invention provides heteromorphic polysaccharide hydrogel microparticles based on microfluidics as well as a preparation method and application thereof, and relates to the technical field of preparation of non-spherical hydrogel microparticles. According to the invention, an oil phase is introduced into the droplet microfluidic device and the phase is located in the middle layer of the coaxial flow direction, and an oil phase containing acetic acid molecules is introduced into the droplet microfluidic device and the phase is located in the outermost layer of the coaxial flow direction. The chitosan-alginic acid homogeneous solution is prepared by the following steps: dissolving chitosan and alginic acid into a special-shaped emulsion template, dripping liquid into a transition pipeline and a sample receiving pipe, diffusing acetic acid molecules in an outermost oil phase into the special-shaped emulsion template to lower the pH value of the chitosan-alginic acid homogeneous solution, so that chitosan is protonized and is subjected to ionic crosslinking with alginic acid carboxyl, thereby realizing the preparation of the special-shaped chitosan-alginic acid polysaccharide hydrogel microparticles. The heteromorphic polysaccharide hydrogel microparticles prepared by the preparation method disclosed by the invention have good morphology stability, a controllable heteromorphic structure and an autofluorescence characteristic. The microparticles have good application prospects in drug delivery carriers, cell encapsulation carriers and tissue engineering carriers.

This invention provides a mussel-inspired biomimetic adhesive and its preparation method. The adhesive is made by uniformly mixing dopamine-modified hyaluronic acid, polydopamine, and gallium nitrate in a specific ratio. The adhesive prepared by this invention is simple to prepare, injectable, and convenient for practical applications. The adhesive prepared by this invention maintains excellent adhesion performance in humid environments and exhibits excellent adhesion performance in humid environments with a pH range of 1–10. This adhesive is non-cytotoxic, and its various properties can be adjusted according to application requirements. It can be applied not only to the bonding of various materials such as glass, metals and their oxides, plastics, and rubber, but also has broad application prospects in clinical medicine, including the bonding and repair of skin or tissue organs, sealing and hemostasis, cell encapsulation, and delivery of bioactive molecules.

The present invention relates to the technical field of biology, and provides a method for enabling a plasma cell to capture an antibody secreted by the plasma cell. Specifically, the method comprises: labeling the plasma cell with a capture reagent; and performing single-cell encapsulation treatment on the labeled plasma cell to capture an antibody, wherein the capture reagent has the activity of binding the antibody secreted by the plasma cell, and the plasma cell has the activity of secreting the antibody. By means of the method, the antibody secreted by the plasma cell can be recaptured onto the membrane surface of the plasma cell to form a structure similar to a BCR of a memory B cell, enabling a corresponding antigen protein to specifically recognize and bind to the BCR on the membrane surface of the plasma cell, thereby enabling screening and sequencing of an antigen-specific plasma cell.

The invention relates to a cell encapsulation system (1), the system comprising at least: a first container (11) intended to contain a solution of cells and a second container (12) intended to contain a solution capable of gelling, a millifluidic or microfluidic encapsulation device (20) connected to the containers for forming cellular microcompartments, of which the outer layer is the solution capable of gelling and the core is the solution of cells, wherein each container is connected to an inlet of the encapsulation device by a dispenser (31, 32) associated with a solution movement system (41, 42) capable of causing the solution contained in the container (11, 12) to flow through the dispenser toward the inlet of the encapsulation device (20), characterized in that the first dispenser (31) has at least one upwardly extending portion.

The present invention is directed to formulations comprising a water-immiscible fluorocarbon solvent and at least one amphiphilic polymer, wherein the least one amphiphilic polymer is selected from a diblock co-polymer surfactant of formula A-B and a triblock co-polymer surfactant of formula B'-A'-B', wherein A and A' independently of each other represent a water-soluble polymer block having a weight average molecular weight in the range of 300 g / mol to 2000 g / mol, and B and B' represent independently of each other a perfluorinated polyether block having a weight average molecular weight in the range of 1500 g / mol to 7500 g / mol. Said formulations exhibit improved properties which render them particularly useful in droplet-based microfluidics applications, including the construction of lipid vesicles, cell encapsulation and monitoring, and droplet digital molecular biology.

The invention discloses a nanometer packaging method of probiotics and obtained nanometer packaged probiotics, and belongs to the technical field of microbial preparations. The nano packaging method provided by the invention comprises the following steps: providing a probiotic bacterial solution, and mixing the probiotic bacterial solution with a ferric ion solution, so that ferric ions are adsorbed on the surfaces of probiotics; mixing the probiotic bacterial liquid adsorbed with the ferric ions with an anion polysaccharide solution rich in carboxyl, and forming a nanogel layer on the surfaces of the probiotics through ionic crosslinking; and mixing the probiotic bacterial liquid coated with the nanogel layer with a polyphenol compound solution, and forming a metal-polyphenol network through coordination complexation, so as to obtain the nano-encapsulated probiotics. The nanogel layer and the metal-polyphenol network are sequentially constructed on the surface of the probiotics, and the synergistic protection mechanism of the nanogel layer and the metal-polyphenol network is utilized, so that efficient single-cell encapsulation of the probiotics is successfully realized, and the survival rate of the probiotics in a gastric acid environment and the colonization ability of the probiotics in intestinal tracts are remarkably improved.

The invention relates to a cell encapsulation system, the system comprising at least: two containers (11, 12), one of which is intended to hold a cell solution and the other a gelling solution; a milli-fluidic or micro-fluidic encapsulation device (13) connected to the containers to form cell microcompartments, the outer layer of which is the gelling solution and the core the cell solution; a collection tank (15) for the cell microcompartments containing a stiffening solution; each container being connected to an inlet of the encapsulation device by one or more distributors (14); and at least one first container (11) being connected to a solution displacement system (P11, P12) capable of introducing a pressurized gas into said container to generate a flow of the solution contained in said container towards the encapsulation device. Figure to be published with the abstract: Fig. 1

The disclosure relates generally to a system, device, and method for cell culturing. In certain embodiments, the system, device, and method may be used to encapsulate single cells in embryo-like, core-shell microcapsules. In some embodiments, microfluidic devices may be utilized to fabricate core-shell hydrogel microcapsules, which may be used to encapsulate individual cells. In some embodiments, the disclosed system and method are utilized to encapsulate cancer stem cells. The disclosed system, device, and method can be used to isolate and culture CSCs, to facilitate the understanding of cancer biology and etiology, and to advance the development of effective CSC-targeted cancer therapies.

This invention relates to the field of biological feed preparation, and discloses a high-yield biological feed for laying hens based on probiotic regulation and its preparation method, comprising: mixing a fermentation substrate containing eggshell powder with a compound microbial agent, the compound microbial agent including Bacillus subtilis and Lactobacillus acidophilus; maintaining the dissolved oxygen concentration inside the fermentation substrate in the range of 20% to 40% by adjusting the external air intake; sealing the fermentation tank and stopping the aeration when the pH value of the fermentation substrate drops to the range of 5.5 to 6.0; triggering the extracellular polymer to undergo a coagulation reaction through an acid-induced mechanism, embedding Lactobacillus acidophilus in situ inside the extracellular polymer to form a microbial cell encapsulation material with acid-permeability resistance; simultaneously utilizing lactic acid to chemically react with the calcium carbonate component in the eggshell powder as a calcium source to generate calcium lactate to achieve the organic conversion of the eggshell calcium source.

The present application provides a kind of based on metal-polyphenol network and light click thiol-alkene gelatin layer layer self-assembly probiotic single cell coating method, first polyphenol is self-assembled with metal ion on the surface of probiotic cell and forms metal-polyphenol network layer, then guide adsorption alkenyl functionalized gelatin on the surface of probiotic cell and form the second layer coating, finally based on the principle of thiol-alkene light click reaction forms crosslinked alkenyl functionalized gelatin protective coating.The probiotic coating method of the present application simplifies the operation steps of existing layer layer self-assembly technology, shortens the encapsulation time, and the number of encapsulation layers is reduced, but the dense protective coating can protect the cell from the damage of gastrointestinal tract adversity environment to maintain the activity of probiotic bacteria, and improve the colonization of probiotic bacteria in intestinal tract.

The present disclosure relates to a modified polymer and a use thereof. Specifically, the present disclosure provides a modified monomer represented by formula I, an alginate polymer containing same, a hydrogel, a capsule, and a corresponding use. The alginate of the present disclosure is used for cell encapsulation, exerting an antifibrotic effect and reducing foreign body reactions.