55 results about "Cell encapsulation" patented technology

The invention belongs to the technical field of biology, and particularly relates to a high-throughput preparation method of a single-cell-derived organoid based on a microcapsule spherical shell structure, and the preparation method comprises the following steps: preparing an aqueous two-phase microcapsule encapsulated with a single cell, culturing the single cell in the aqueous two-phase microcapsule to obtain the organoid, the substances of the inner phase and / or the outer phase respectively comprise any one or a combination of more of the following substances: methacrylated gelatin, carboxymethyl cellulose, glucan, sodium alginate and polyvinyl alcohol. The invention also provides the organoid prepared by the preparation method and application of the preparation method. According to the invention, the microcapsule with a permeable core-shell structure is formed by packaging the microcapsule in a single-cell form in two-aqueous-phase liquid drops, the wrapping rate is effectively controlled and adjusted on the premise of conforming to Poisson distribution by optimizing parameters, and the single-cell wrapping rate and the organoid single-cell induction efficiency are optimized.

The embodiment of the invention provides an encapsulated cell droplet sorting method and system, electronic equipment and a storage medium, and belongs to the technical field of intelligent biomedical engineering. The method comprises the following steps: acquiring a sorting area image of a micro-fluidic channel in a micro-fluidic chip; cutting the sorting area image according to a preset cutting frame to obtain a microfluidic droplet image; the microfluidic liquid drop image is input into a cell recognition model based on a convolutional neural network structure to obtain a cell recognition result, and the cell recognition result is used for judging whether cells exist in liquid drops or not; and determining a chip control signal of the micro-fluidic chip according to the cell recognition result so as to collect encapsulated cell droplets in the sorting area. According to the invention, the droplet cell encapsulation efficiency can be improved.

The invention relates to a microbial single-cell gelation encapsulation method based on aloe gel and application, the method realizes single-cell gelation encapsulation through electrostatic interaction of sterile aloe gel and intestinal anaerobic bacteria (such as Agathobacter rectasis), and the method comprises the specific steps of aloe gel preparation, bacterial suspension culture and oscillation mixing to form a stable structure. The preparation method has the advantages that the acid resistance and biocompatibility of the natural aloe gel are utilized to replace a traditional synthetic material, so that the gastrointestinal tolerance of thalli is improved; meanwhile, the synergistic effect of aloe active ingredients (such as aloe-emodin) and intestinal bacterial metabolites (butyric acid) is combined, so that the anti-inflammatory and intestinal steady-state regulation and control effects are enhanced. Experiments show that the survival rate of the encapsulated bacteria in simulated gastric juice within 4 hours is larger than or equal to 90%, the intestinal colonization rate is effectively increased compared with naked bacteria, the inflammatory factor level of a colitis model mouse can be remarkably reduced, and a safe and efficient new strategy is provided for micro-ecological treatment of inflammatory bowel diseases.

An electrochemical cell, including: a first electrode, a second electrode, a first connecting conductor, a second connecting conductor, a cell encapsulation, the first electrode and the second electrode being arranged inside the cell encapsulation and insulated with respect to the surrounding area, preferably in a fluid-tight manner, by the cell encapsulation, and the first connecting conductor and the second connecting conductor protruding from the cell encapsulation. It is provided that the first connecting conductor and the second connecting conductor are each, at least in sections, insulated with respect to the surrounding area, preferably in a fluid-tight manner, by an insulation, the insulated regions of the first connecting conductor and of the second connecting conductor protruding from the cell encapsulation, and a respective terminal section of the first connecting conductor and of the second connecting conductor not being covered by the insulation.

The present disclosure relates to a modified polymer and a use thereof. Specifically, the present disclosure provides a modified monomer as shown in formula I, a polymer containing same, a hydrogel, a capsule, and a corresponding use. An alginate of the present disclosure is used for cell encapsulation, and can provide an anti-fibrotic effect and reduce foreign body reaction.

The invention discloses a hydrophobic polymer, a preparation method thereof and a single cell encapsulation method based on interaction of a subject and an object. The hydrophobic polymer disclosed by the invention can be embedded into a cell membrane to carry out hydrophobic modification on cells, and anchoring groups are modified on the surfaces of the cells, so that single-cell encapsulation of the cells is facilitated. The single cell encapsulation method based on interaction of the subject and the object comprises the following steps: 1) preparing hydrophobic polymer intercalation modified cells; and 2) carrying out layer-by-layer packaging on the hydrophobic polymer intercalation modified cells by using the cyclodextrin grafted hyaluronic acid and the adamantane grafted hyaluronic acid. The single cell encapsulation method based on host-guest interaction has the advantages of high encapsulation efficiency, high cell survival rate, good biocompatibility, simplicity in operation, mild reaction process, high reaction speed and the like, is suitable for large-scale industrial application, and cells obtained by encapsulation can be used for tissue repair in multiple aspects and are wide in application range.

An implantable therapeutic delivery device comprising a porous pouch and a cell encapsulation device configured to be housed in the porous pouch. The porous pouch can comprise a bioabsorbable material and / or a vascularization promoter, and the porous pouch can be packaged separately from the cell encapsulation device.

The invention relates to hydrogel with ice recrystallization inhibiting activity, a preparation method of the hydrogel and application of the hydrogel in cell cryopreservation. Firstly, novel hydrogel is provided; a polysaccharide-PVA graft and sulfhydrylation modified gelatin are mixed to prepare the hydrogel with ice recrystallization inhibiting activity, in-situ cell encapsulation culture verifies that the hydrogel has good cell compatibility and can maintain the proliferation performance of cells, and the hydrogel is suitable for cryopreservation of a cell-hydrogel structure after long-term culture and has good application prospects. And the biological activity of the cells is maintained.

This application provides a cell encapsulation stromal material and its manufacturing method and application. The material is prepared by cutting, low-temperature microparticulation grinding, enzymatic decomposition, cell component removal, virus inactivation, freeze-drying and radiation sterilization using cartilage from mammals such as pigs, cows, sheep, horses and deer. The method is not for diagnostic purposes, and the stromal material is used for 3D bioprinting alone or with encapsulated cells. The main components of the material are type II collagen, chondroitin sulfate and hyaluronic acid, and have the advantages of no crosslinking toxicity, low immunogenicity, good biocompatibility and can be used for human implantation. In terms of structure, it has a good surface topology structure, provides a tissue microenvironment similar to that in the body for in vitro cell culture, and can be used as a microcarrier for cell culture, as well as promote the formation of cell mass, and can encapsulate cells in the gel-state stromal during the process of cell storage and transportation, protect cells from the effects of adverse environments and improve the viability of cells.

The invention relates to the technical field of biological agents, in particular to a Michael addition reaction mediated polysaccharide and probiotic surface covalent coupling single cell encapsulation method. The method comprises the following steps: adding polysaccharide into dimethyl sulfoxide for dissolving, adding triethylamine and methacrylic anhydride to obtain modified polysaccharide containing alpha, beta-unsaturated ester, adding activated probiotic suspension into an HEPES buffer solution, adding a modified polysaccharide solution containing alpha, beta-unsaturated ester into the vortex-dispersed probiotic suspension, oscillating and incubating to obtain the probiotic suspension containing alpha, beta-unsaturated ester. Preparing a probiotic suspension of the nano coating encapsulated by the polysaccharide; according to the method, a stable and firm protective layer is formed on the surface of the probiotics, the activity and functions of natural bacteria are kept, meanwhile, the conveying and colonization survival efficiency of the probiotics in the intestinal tract is effectively improved through a compact polysaccharide structure on the surface of the probiotics, and the commercial application value of the probiotics in the fields of food and medicine is improved.

The invention relates to the technical field of cell packaging and counting, in particular to a micro-fluidic chip and a cell counting system. A sample hole, a dye hole, a waste liquid hole and a dilution hole are formed in the micro-fluidic chip, a sample micro-channel, a dye micro-channel, a waste liquid channel and a dilution micro-channel are arranged in the micro-fluidic chip, the sample hole is communicated with the sample micro-channel, the dye hole is communicated with the dye micro-channel, the waste liquid hole is communicated with the waste liquid channel, and the dilution hole is communicated with the dilution micro-channel; a dilution cavity, a uniform mixing micro-channel and a photographing observation cavity are also arranged in the micro-fluidic chip. According to the micro-fluidic chip disclosed by the invention, through integrated design and ingenious design of the positions and connection relations of the dilution cavity, the uniform mixing micro-channel and the photographing observation cavity, the steps of sample dilution, dyeing, observation and the like are successfully integrated into one micro-fluidic chip, so that a full-automatic process from sample introduction to final counting is realized, the operation flow is greatly simplified, and the working efficiency is improved. And the cell counting efficiency and accuracy are greatly improved.

A fish liver decellularized extracellular matrix based microfluidic 3D printing hydrogel for liver regeneration, and a preparation method thereof are provided. A fish liver decellularized extracellular matrix (dECM) is combined with gelatin methacryloyl (GelMA), and loaded with hepatic spheroids derived from induced pluripotent stem cells (iPSC-hep) for liver regeneration. The fish liver decellularized extracellular matrix based microfluidic 3D printing hydrogel of the present disclosure has excellent biocompatibility and retains intact endogenous growth factors, maintains the biological activity of cells, ensures effective cell encapsulation, and is conducive to robust functional expression of iPSC-hep. After being transplanted in vivo, the hydrogel significantly improves the survival rate and liver function of mice with acute liver failure, and promotes liver regeneration and repair.

A cell encapsulation device for implantation in a body includes one or more cell encapsulation layers, each of the one or more cell encapsulation layers including at least one membrane and a guide tube. The at least one membrane is semi-permeable. The at least one membrane forms a chamber for encapsulating cells and at least one entry port through the at least one membrane. The guide tube extends from the at least one entry port into the chamber. The guide tube includes a porous wall along at least a portion of its length. The guide tube is capable of guiding movement of a catheter inside the chamber.

The present invention discloses a polyurethane ultra-thin hierarchical porous membrane that can be used for cell encapsulation and its manufacturing method and application. The method comprises the following steps: (1) polyurethane is dissolved in dimethyl sulfoxide, and then deaerated; (2) an aluminum plate is pre-cooled using a refrigeration cycle machine; (3) the obtained polyurethane solution is added dropwise on a glass plate, and then excess solution is scraped off with a film-laying device; (4) the glass plate is placed on a pre-cooled aluminum plate; (5) after observing that the solution crystallizes, the glass plate is placed in warm water; (6) the film is taken out of the water and dried. The method of the present invention is simple, overcomes the problem that the unidirectional freezing aperture is larger, and the obtained microporous membrane pore diameter is within the range of 500nm-5μm, and the film-making conditions are adjusted to obtain the desired pore diameter. The porous membrane of the present invention has a large number of longitudinal through-holes, and in an ultra-thin thickness (100μm), a hierarchical structure is realized, with a supporting layer with a large aperture, and a barrier layer with a small aperture, which is conducive to controlling the permeation isolation molecular weight.

Methods and apparatus for acoustically assisted droplet generation with or without cell encapsulation.

The invention relates to a microfluidic chip (1) consisting of a body (2), a primary inlet (5) and at least one secondary inlet (6) non-coaxial to the primary inlet (5), each primary inlet (5) and secondary inlet (6) for receiving a substance, and comprising an outlet (7), the primary inlet (5) being connected to the outlet (7) via a primary channel (8), the main channel has a substantially straight portion defining a central axis Z of the microfluidic chip (1), and the secondary inlet is also connected to the outlet (7) via a secondary channel (9). According to the invention, the secondary channel (9) is divided into at least two secondary ducts (11) of the same size, which converge in a coaxial annular injection duct with the primary channel (8) in an equally distributed manner about a central axis Z.

The present invention relates to the field of photovoltaic cell packaging adhesive films, and provides an anti-PID type EVA adhesive film, the anti-PID type EVA adhesive film comprises EVA resin, a cross-linking agent, an assistant cross-linking agent, a grafting monomer and a functional auxiliary agent, the grafting monomer comprises high-functional-group acrylate and low-functional-group acrylate, the unsaturated double-bond functional group of the high-functional-group acrylate is greater than or equal to 3, and the unsaturated double-bond functional group of the low-functional-group acrylate is greater than or equal to 2. Unsaturated double-bond functional groups of the low-functional-group acrylate are less than or equal to 2, and the low-functional-group acrylate not only can reduce the viscosity of the high-functional-group acrylate, but also has certain compatibilization and swelling effects on the EVA resin, so that the compatibility of the high-functional-group acrylate and the EVA resin can be enhanced; absorption and uniform dispersion of the high-functional-group acrylate in the EVA resin are facilitated, so that the compactness of the EVA adhesive film after crosslinking is improved, and the anti-PID effect of the EVA adhesive film is improved.

The invention provides a cell packaging oxygen production device and application thereof, and belongs to the technical field of implantable medical instruments, the cell packaging oxygen production device comprises: a raw material storage module for storing a hydrogen peroxide solution; the diffusion control module is arranged between the raw material storage module and the cell packaging module and comprises a catalytic cavity and an isolation structure, and a catalyst is arranged in the catalytic cavity and used for catalyzing hydrogen peroxide molecules to generate oxygen; the isolation structure comprises a first isolation membrane and a second isolation membrane, and the first isolation membrane is arranged between the raw material storage module and the catalytic cavity and controls hydrogen peroxide molecules to diffuse into the catalytic cavity; the second isolating membrane is arranged between the catalytic cavity and the cell packaging module and is used for controlling oxygen to diffuse into the cell packaging module; the cell encapsulation module contains a cell encapsulation matrix containing cells and is used for receiving oxygen and supplying oxygen to the cells. The cell packaging oxygen production device can control the oxygen production rate and match the oxygen consumption demand of cells, effectively avoids the toxicity of hydrogen peroxide and the migration risk of a catalyst, and is excellent in biological safety.

A soft package lithium ion battery comprises an outer bag formed by folding a soft package film, and the outer bag comprises a battery cell packaging area located in the middle and used for containing a soft package battery cell, a first air bag located on the left side of the battery cell packaging area and a second air bag located on the right side of the battery cell packaging area. The tops of the first air bag and the second air bag are packaged by a top sealing area in a heat sealing mode, the left side of the first air bag is packaged by a first side sealing area in a heat sealing mode, the right side of the second air bag is packaged by a second side sealing area in a heat sealing mode, and an isolation pre-sealing line parallel to the second side sealing area is arranged on the left side of the second air bag and close to the battery cell containing area. The isolation pre-sealing line is formed by gap heat sealing, the isolation pre-sealing line comprises an edge sealing area composed of a plurality of sealing edges which are sequentially arranged at intervals, and an unsealed area is formed between every two adjacent edge sealing points. According to the utility model, the extraction amounts of electrolyte and gas on the two sides of the soft package battery cell are almost the same in the secondary sealing process, so that the hardness of the two sides of the battery cell is consistent, and the internal consistency of the battery cell is better.

The invention relates to the field of photovoltaic cell packaging adhesive films, and provides a damp-heat-resistant packaging adhesive film for an N-type TOPCon cell, the damp-heat-resistant packaging adhesive film comprises a matrix resin and a functional additive, the matrix resin is a vinyl copolymer, the functional additive comprises an acid acceptor and a corrosion inhibitor, the acid acceptor is a compound containing an epoxy group, and the corrosion inhibitor is a compound containing an epoxy group. The number of epoxy functional groups in the compound containing the epoxy groups is greater than or equal to 2, and the corrosion inhibitor is a compound containing a benzotriazole group. Wherein the corrosion inhibitor can gradually migrate to the surface of the damp-heat-resistant packaging adhesive film, so that the damp-heat-resistant packaging adhesive film can gradually become a structure of a base body layer and an anti-corrosion layer arranged on the surface of one side of the base body layer along with the increase of time. The acid acceptor and the corrosion inhibitor are combined, so that the problem that the battery is corroded by substances such as water vapor and acid can be effectively solved.

Provided are novel serum-free culture media which comprise basic fibroblast growth factor (bFGF), transforming growth factor beta-3 and ascorbic acid at a concentration of at least about 50 microgram / ml; ascorbic acid at a concentration range of about 400-600 microgram / ml, bFGF at a concentration range of about 50-200 ng / ml, xeno-free serum replacement and a lipid mixture; the IL6RIL6 chimera at a concentration range of about 50-200 picogram per milliliter (pg / ml); or leukemia inhibitory factor (LIF) at a concentration of at least 2000 units / ml; cell cultures comprising same with pluripotent stem cells such as human embryonic stem cells and induced pluripotent stem (iPS) cells, and methods of using same for expanding pluripotent stem cells in an undifferentiated state using two-dimensional or three-dimensional culture systems; and methods of expanding iPS cells in a suspension culture devoid of substrate adherence and cell encapsulation.

Thin film devices, e.g., multilayer thin film devices, that encapsulate cells for transplantation into a subject are provided. Also provided are methods of using and methods of preparing the subject devices. The thin film devices include a first porous polymer layer and a second porous polymer layer that define a lumen therebetween and encapsulate a population of cells within the lumen. The thin film devices can promote vascularization into the lumen of the device via the pores in the first polymer layer and / or second polymer layer; limit foreign body response to the device; limit ingress of cells, immunoglobulins, and cytokines into the lumen via the first and the second polymer layers; and release from the first polymer layer and / or the second polymer layer molecules secreted by the population of cells.

The invention relates to a cell encapsulation system (1), the system comprising at least: a first container (11) for holding a cell solution and a second container (12) for holding a gelling solution, a milli-fluidic or micro-fluidic encapsulation device (20) connected to the containers to form cell microcompartments, the outer layer of which is the gelling solution and the core the cell solution, wherein each container is connected to an inlet of the encapsulation device by a distributor (31, 32) associated with a solution displacement system (41, 42) capable of causing the solution contained in said container (11, 12) to flow from said distributor to the inlet of the encapsulation device (20), characterized in that the first distributor (31) has at least one upward-extending portion. Figure to be published with the abstract: Fig. 1

The invention relates to a method for dynamically encapsulating microbial cells by using a hydrogen bond organic framework material, and belongs to the technical field of cell encapsulation. The method comprises the following steps: preparing nano HOF, dispersing cells in a culture medium, adding a nano HOF aqueous dispersion, culturing, and dynamically packaging proliferative cells by the HOF. According to the invention, a hydrogen bond organic framework material monomer containing carboxyl is selected to encapsulate cells, self-assembly of organic molecule units on the surfaces of the cells is realized through a dynamic hydrogen bond network, and highly reversible hydrogen bonds interact to construct an intelligent encapsulation shell with a controllable disassembly / assembly function; and continuous packaging protection in the whole cell proliferation process can be realized.

The present invention discloses a zwitterionic supramolecular hydrogel based on anion-π and its preparation method and application. The hydrogel is self-assembled by a zwitterionic copolymer and a p-styrene sulfonic acid copolymer. In the present invention, the supramolecular hydrogel only needs to be rapidly formed by simply mechanically mixing the zwitterionic polymer copolymer and the p-styrene sulfonate copolymer solution, and the dynamic cross-linking characteristics give it excellent injectability and self-repairing properties. This hydrogel is composed of zwitterions with excellent immune compatibility and poly (p-styrene sulfonate) with heparin-like properties, so it has excellent immune compatibility and can play a role in various in vivo application scenarios. In addition, this dynamic cross-linking network based on anion-π interaction and the heparin-like structure are very beneficial to the preservation of proteins and have broad application prospects in the fields of protein delivery and cell encapsulation.

A therapeutic device includes a pouch having opposed first and second ends and first and second composite layers extending between the opposed first and second ends. The first composite layer includes a first cell permeable layer extending between the opposed first and second ends and a first cell retentive layer extending between the opposed first and second ends. The pouch includes a reservoir formed between the first and second composite layers, contacting the first cell retentive layer, and at least one port in fluid communication with the reservoir. The therapeutic device further includes a removal element configured to operably engage the first end of the pouch, to cause the first end to be moveable towards the second end by eversion. In some embodiments, each of the first and second composite layers includes a plurality of wrinkles that are incrementally disengaged from a tissue to remove the therapeutic device atraumatically.

This invention provides a photovoltaic cell encapsulation structure and preparation method for improving weather resistance and heat dissipation efficiency. The photovoltaic cell encapsulation structure includes at least: a photovoltaic cell layer; a first encapsulation layer disposed on the light-receiving side of the photovoltaic cell layer, the first encapsulation layer containing a chemically bonded compound of organosilicon resin and nano-silica; and a second encapsulation layer disposed on the backlight side of the photovoltaic cell layer, the second encapsulation layer containing a blend of a polymer matrix and a thermally conductive filler. This invention achieves significant results in extending module lifespan, improving heat dissipation performance, and maintaining process compatibility through a dual-layer functional design of "weather resistance on top and thermal conductivity on the bottom."

The invention relates to a cell encapsulation system (1) comprising: a container (11) of a cell solution, a second container (12) of a solution suitable for gelling, an encapsulation device (20) for forming cell microcompartments, in which the containers are connected to the encapsulation device by distributors (31, 32) associated with solution displacement systems (41, 42); and comprising a control unit (4) arranged to, prior to a production step: in a priming step (E32) of the first distributor, control the displacement system (41) to circulate a portion of the cell solution in this distributor (31); in a priming step (E33) of the second distributor subsequent to the priming step (E32), control the displacement system (42) to circulate a portion of the solution suitable for gelling in this distributor (32). Figure to be published with the abbreviation: Fig. 1

The present disclosure provides methods of detecting cell cystic canals in tissue of a subject for cancer diagnosis, screening, and metastasis prediction.

The invention relates to a preparation method of a fluorescent probe for detecting the viscosity of a cell membrane in three-dimensional hydrogel, the preparation method comprises the following steps: synthesizing an intermediate Br-BODIPY, reacting the Br-BODIPY with N-methyldiallylamine in toluene, precipitating and purifying to obtain a target product fluorescent probe BODIPY-C. The fluorescent probe BODIPY-C has a viscosity sensitive rotor BODIPY and a photopolymerizable vinyl group, and can be used for detecting the viscosity of the cell membrane in the three-dimensional hydrogel. Covalent grafting to photo-crosslinkable hydrogel can be promoted through ultraviolet-initiated polymerization; the invention further discloses a fluorescent probe application method, after BODIPY-C and HM hydrogel are subjected to an ultraviolet light crosslinking reaction, BODIPY-C is combined with an HM hydrogel network in a covalent grafting mode, when the BODIPY-C is applied to a three-dimensional hydrogel system for encapsulating cells, in a fluorescent probe grafted hydrogel cell encapsulation construction body, the fluorescent probe BODIPY-C is positioned on a cell membrane in the construction body, and in the three-dimensional hydrogel system for encapsulating the cells, the fluorescent probe BODIPY-C is positioned on a cell membrane in the construction body. The change of the cell membrane viscosity can be monitored in real time through a BODIPY-C fluorescence signal, and the method is good in detection effect, can reflect the real condition of a three-dimensional culture system, and can be applied to the field of fluorescence probe detection.