73 results about "Droplet microfluidics" patented technology

The invention belongs to the technical field of microfluidics, and particularly relates to a liquid separation-convergence integrated liquid drop micro-fluidic chip and a liquid drop preparation method.The liquid separation-convergence integrated liquid drop micro-fluidic chip is characterized in that a liquid drop generation unit is arranged at the communication position of a branch splitting channel and a convergence channel which is correspondingly communicated with the branch splitting channel; and under the action of continuous phase shearing force, the dispersed phase solution is sheared off by the continuous phase solution, so that liquid drops are formed. The droplet generation units are symmetrically arranged along the circumference of the channel, the distance from each unit to the center is equal, and a continuous phase solution and a dispersed phase solution encountered at each droplet generation unit are almost equivalent, so that the sizes of the generated droplets are consistent, the droplets with basically consistent sizes are obtained at a collection port, and high-flux preparation of microspheres with uniform sizes is realized.

The invention belongs to the technical field of cosmetics, and particularly relates to a water-in-oil-in-water three-phase coated microbead as well as a preparation method and application thereof. The water-in-oil-in-water three-phase coated microbead is of a spherical structure and sequentially comprises an outer water phase, an inner oil phase and an inner water phase from outside to inside, the outer water phase comprises sodium alginate, agarose, cyclodextrin, glycerin and other components, the inner oil phase comprises caprylic / capric triglyceride, grape seed oil, olive squalane, tocopherol and other components, and the inner water phase comprises sodium alginate, agarose, cyclodextrin, glycerin and other components. And the inner water phase comprises ampelopsis grossedentata extract, astragalus membranacus root extract and other components. According to the invention, an easily oxidized and unstable active matter is fully and firmly protected by adopting a droplet microfluidic coating technology, so that the active matter is free from external stimulation, active ingredients with different effects play multiple roles, and the composition has good anti-inflammatory, soothing and oil-controlling effects and can be applied to the field of cosmetics.

The invention belongs to the technical field of microfluidics, and particularly relates to an integrated liquid drop micro-fluidic chip based on 3D printing and a liquid drop preparation method. Comprising a continuous phase channel unit and a dispersed phase channel unit, the continuous phase channel unit comprises a main channel unit, and a plurality of main split channels are communicated in the circumferential direction around an outlet of the main channel unit; the dispersed phase channel unit comprises a branch channel unit, a plurality of branch splitting channels are circumferentially communicated around an outlet of the branch channel unit, a droplet control structure is arranged at the communication part of the main splitting channel and the outlet of the branch splitting channel, and a droplet generation channel is arranged at the communication part of the outlet of the branch splitting channel and the outlet of the main splitting channel; an outlet of each liquid drop generation channel is communicated with a collection opening; the multiple collection openings communicate with the amplification section structure, and an outlet of the amplification section structure communicates with a total collection opening. The three-dimensional space is fully utilized, high-flux and uniform preparation of the liquid drops can be achieved, and the method is suitable for large-scale production of the liquid drops.

The invention discloses a hydrogel microsphere sorting method, an artificial organ preparation method, a system and a medium. The method comprises the following steps: acquiring a hydrogel microsphere image in the micro-fluidic chip; analyzing the hydrogel microsphere image through a neural network to obtain an analysis result; based on the analysis result, hydrogel microspheres wrapping the target cells are sorted out; the neural network comprises a feature extraction layer and a multi-scale joint output head, and the hydrogel microsphere image is processed by the multi-scale joint output head after being convolved by the feature extraction layer to obtain an analysis result. According to the method, the hydrogel microspheres wrapped with the target cells are accurately identified and sorted by using a droplet microfluidic technology, a standardized artificial organ can be prepared based on the sorted hydrogel microspheres, and the functional maturity and reliability of the artificial organ are remarkably improved.

The utility model provides a digital droplet micro-fluidic chip system and relates to the technical field of microbiological detection. The digital liquid drop micro-fluidic chip system comprises a micro-fluidic liquid drop generation chip, a micro-fluidic liquid drop fusion chip and a micro-fluidic liquid drop reaction chip. The microfluidic liquid drop generation chip comprises n liquid drop generation units, wherein each liquid drop generation unit comprises a first dispersed phase inlet, a second dispersed phase inlet, a dispersed phase mixing channel and a liquid drop generation area; the dispersed phase mixing channel is of a snakelike structure, a broken line structure or a spiral structure; the liquid drop generation area comprises a cross intersection, and the cross intersection is of a closing-up structure which is in a trapezoid shape. The digital droplet micro-fluidic chip system disclosed by the utility model has the advantages of high detection flux, high stability, high sensitivity, rapidness, low cost and the like, is suitable for simultaneously detecting various bacteria in an unknown sample, and can be applied to the fields of environmental microorganism detection, food-borne pathogenic bacterium detection, clinical pathogenic bacterium diagnosis and the like.

The present application relates to the technical field of microfluidic chip, and disclose a centrifugal microfluidic chip for generating different size droplets simultaneously, including a liquid distribution channel for uniformly distributing sample to multiple droplet generation units, the design of a quantitative pool ensures the fixed volume of liquid and the consistency of the number of droplet generation, the chip contains a sample inlet and two continuous phase reagent inlets, the sample is precisely distributed to the quantitative pool by centrifugal force and enters the droplet generation units respectively, the first droplet generation unit is used for generating large size droplets with a diameter of 100-200 mu m, and the second droplet generation unit is used for generating small size droplets with a diameter of 10-100 mu m, the microfluidic chip can realize the preparation of ultrahigh throughput droplets, the generated droplets are guided to the collection area by centrifugal force to form a regular single-layer arrangement, the chip has wide application in the fields of biomedical research, material science, chemical synthesis and environmental monitoring, especially in the preparation of fluorescent microspheres, which can improve the experimental efficiency and result consistency.

The invention discloses an electrically-driven integrated liquid drop microfluidic device, relates to the technical field of microfluidics, and is used for solving the technical problems that most of the existing control modes are limited to plane control and cannot adapt to unstructured working environments in a deep cavity and a biochemical analyzer. The electrically-driven integrated liquid drop microfluidic device comprises a supporting plate, a liquid collecting plate, a detection platform, a displacement mechanism and a liquid transferring mechanism, the supporting plate is provided with a first surface and a second surface which are oppositely arranged; the supporting plate is arranged on the fixed platform through the first surface; the liquid collecting plate is arranged on the supporting plate through the second surface; the detection platform is arranged at any end of the liquid collection plate; the displacement mechanism is arranged to move in the horizontal and / or vertical direction relative to the liquid collecting plate; the pipetting mechanism is arranged on the displacement mechanism and is used for moving the liquid drops or the liquid marbles from the liquid collection area to the detection platform through dielectrophoretic force.

The application discloses a kind of based on digital droplet microfluidic multi-sample flux multi-index detection device and application thereof, belong to electromechanical device, microfluidic chip and immunoprotein analysis field.The device includes droplet microfluidic equipment and immunoprotein analysis chip component, droplet microfluidic equipment is used to drive multiple droplets, mainly divided into three major systems: power supply system, computer operating system and chip clamp system, immunoprotein analysis chip component includes biological analysis substrate and microarray chip.The present application drives micro liquid sample by digital droplet microfluidic technology, realizes the multi-index detection of automated immunoprotein analysis chip.The detection device of the present application has a series of advantages such as rapidity, independence, low consumption, automation, function integration and multi-index detection, and is expected to become the most fruitful rapid diagnostic device for improving tumor diagnosis positive rate.

Single-cell methods are provided for investigating DNA and RNA interactions with regulatory proteins and evaluating the cytotoxicity of immune cells towards target cells, such as tumor cells. The methods utilize droplet microfluidics and imaging-based technology to facilitate the isolation and analysis of protein-bound nucleic acids from individual immune cells or apoptotic tumor cells. The methods utilize fluorescence-activated droplet sorting and are capable of isolating and characterizing mitochondrial DNA from cytotoxic immune cells.

The invention discloses a method and a device for preparing liquid drops by using a liquid drop generating device in the technical field of liquid drop microfluidics, and the device comprises a liquid drop receiving tank for containing continuous phase liquid, a liquid drop generating mechanism for producing the liquid drops and a micro-vibration mechanism for providing a vibration source, wherein the vibration output direction of the piezoelectric actuator is perpendicular to or approximately perpendicular to the length direction of the pointed-end pipe, so that the continuous-phase liquid near the nozzle can conveniently form an acoustic vortex effect to promote the formation of liquid drops. The method for preparing the liquid drops comprises the following steps: S1, respectively preparing a dispersed phase liquid and a continuous phase liquid; s2, extending a nozzle to a depth h below the liquid level of the continuous-phase liquid; s3, working parameters of the piezoelectric actuator are set, the working parameters comprise the voltage U and the sine wave frequency f1, the voltage U ranges from 20 V to 120 V, and the sine wave frequency f1 ranges from 2 kHz to 20 kHz. By triggering the local acoustic vortex effect in the continuous phase, the formation of the liquid drops is promoted, and the purposes of reducing the particle size variation of the liquid drops and improving the production efficiency of the liquid drops are achieved.

The invention relates to the technical field of micro-nano sensing and detection, in particular to a temperature sensing method based on a micro-nano robot. The invention discloses a temperature detection method based on a micro-nano robot. The temperature detection method comprises the following steps: step 1, preparing a temperature sensitive material by using copper tetraamminesulfate particles and resin; 2, preparing a micro-nano robot with a temperature detection function by adopting a droplet microfluidic technology and a physical vapor deposition technology based on the temperature sensitive material in the step 1; 3, extracting color features by using the micro-nano robot with the temperature detection function obtained in the step 2, and building a temperature detection regression prediction model based on the color features; and 4, on the basis of the temperature regression prediction model obtained in the step 3, the micro-nano robot with the temperature detection function is used for carrying out temperature detection on the micro gap, and the average value of the detection results of the multiple micro-nano robots is taken as the final detection temperature. The problem that an existing temperature detection method in the industrial field is limited is solved.

Provided are a single-cell chromatin accessibility and transcriptome combined sequencing method and application thereof. The method comprises: treating a cell nucleus using a transposase to obtain gDNA of a chromatin open region connected with a first specific tag sequence; treating the cell nucleus using a transcriptome capture sequence to obtain cDNA connected with a second specific tag sequence; generating a droplet based on a droplet microfluidic to seal the cell nucleus and a first microbead in the droplet, wherein the gDNA and the cDNA are captured by the first microbead, and wherein more than one cell nucleus is sealed in the droplet; and sequencing the gDNA and the cDNA to obtain combined information of single-cell chromatin accessibility and transcriptome according to the first specific tag sequence and the second specific tag.

The application discloses a polar microorganism separation and culture method based on a droplet microfluidic technology. The method comprises the following steps: obtaining a polar sample and performing oscillation extraction at low temperature to obtain a sample suspension; determining a plate cultivable rate R through a solid plate culture method; based on the R and a preset target number of cultivable cells N, calculating a target total number of cells T required by a droplet microfluidic experiment, and preparing a sample with a corresponding cell concentration for machine loading; generating single-cell-wrapped microdroplets through a droplet microfluidic system for the sample for machine loading, and performing long-term culture at low temperature; and screening positive droplets and purifying strains after the culture. Through the cultivable rate feedback, the sample concentration for the microfluidic loading is precisely optimized, the separation efficiency of the polar difficult-to-culture microorganism is significantly improved, the inter-species competition inhibition of the traditional plate is broken, and the diversity and novel group proportion of the obtained strains are greatly improved.

The application discloses a droplet microfluidic sorting method, a sorting system, an electronic device and a storage medium. The droplet microfluidic sorting method comprises the following steps: acquiring a first time point of positive droplet trigger sorting in a microfluidic device; determining a second time point of the positive droplet reaching an outlet of a collection channel according to the first time point and a time length of the positive droplet passing through the collection channel to reach the outlet of the collection channel; the outlet of the collection channel is used for forming an oil droplet; and comparing an oil droplet formation time point closest to the first time point with the second time point to determine a target oil droplet containing the positive droplet. The droplet microfluidic sorting method realizes the automation of sorting a single target droplet, further sorts the target liquid into a droplet containing a single target bacterium or cell, avoids the loss of cells in the demulsification process or the phenomenon that target cells do not exist in part of the well plates or multiple target cells exist in part of the well plates, and thus the efficiency of target sequence acquisition is improved.

The application provides a method for detecting secretion expression of a target protein, which comprises the following steps: fusing the target protein with a fluorescence-enhanced nanobody to obtain a fusion protein; adding the obtained fusion protein into a system containing a fluorescent protein and a fluorescence-inhibited nanobody to perform a reaction; and measuring the fluorescence intensity of the system after the reaction is completed, and calculating the secretion expression amount of the target protein according to the change amount of the fluorescence intensity. In the application, the GBP1 is fused with the target protein, and the expression interference of the GBP1 on the target protein is reduced by adding a connecting peptide, so that the secretion expression amount of the target protein is determined. The method of the application can detect a wide concentration range of the target protein, has a high signal response multiple, and can be applied to a shake flask, a well plate and a droplet microfluidic screening system.

1. Name of the product in this design: Main body of a droplet microfluidic cell sorter. 2. Application of this design: The main body of the droplet microfluidic cell sorter is used for sorting and screening cells. 3. The key design features of this product are its main shape and the pattern of its main view. 4. The image or photograph that best illustrates the design's key points: a 3D model.

The present application relates to the field of droplet microfluidics, and more particularly to a droplet reading chip and a method of using the same. The disclosed droplet reading chip comprises a microcavity for tiling droplets and a pipette for pipetting droplets into the microcavity. The chip can tile a large number of droplets at the same time, meeting the requirement of high-throughput detection of droplets. In addition, the chip can keep the droplets in a relatively closed space during detection, reducing the risk of contamination.

The application provides a method for preparing hollow non-spherical micro-nanoparticles based on a coaxial micro-reactor, and belongs to the field of droplet microfluidics and polymer materials. A three-channel coaxial micro-reactor is designed to generate a water-in-oil-in-water (W / O / W) double emulsion. The innermost phase is an alkaline electrolyte aqueous solution, the intermediate oil phase is a polymer solution, and the outermost phase is a surfactant aqueous solution. With the volatilization of the intermediate phase solvent, the double emulsion droplets form hollow non-spherical micro-nanoparticles. The double emulsion is placed in a collection liquid with different electrolyte concentrations, water molecules in the collection liquid penetrate into the innermost phase under the action of osmotic pressure, increase the size of the inner droplets of the double emulsion, and then change the morphology of the outer droplets, so that the non-spherical structure required is presented, and the size of the hollow micro-nanoparticles is controllable. The coaxial micro-channel reactor used in the application adopts a flow focusing structure, which is used for controllable generation of double emulsion to prepare multi-chamber hollow non-spherical micro-nanoparticles.

The application discloses ecdysterone-low molecular weight fructan composite microspheres with fat-reducing and muscle-increasing functions, and a preparation method and application thereof, and belongs to the technical field of biological medicine and functional food. The composite microspheres have a core-shell structure, the inner core is a calcium alginate gel, the outer shell is chitosan, the inner core co-encapsulates ecdysterone and low molecular weight fructan, and the mass ratio of the two is 0.8-1.2:1. The preparation adopts a liquid drop microfluidic technology combined with an internal gelation method to form the inner core, and adopts a post-crosslinking method to coat the outer shell. The composite microspheres are regular in shape, uniform in particle size, high in encapsulation rate, good in stability, excellent in pH response slow-release and anti-degradation characteristics in the gastrointestinal tract. In-vivo pharmacological experiments prove that the composite microspheres can reduce the body weight and body fat rate of obese mice, increase the lean meat content and muscle strength, realize synergistic effects of fat reduction and muscle increase, and can be used for preparing drugs or functional food for improving obesity-related muscle loss, low-muscle obesity and related metabolic syndrome.

Microfluidics technology incorporating 3D gel droplets is provided for analysis of tumor cells and their interactions with immune cells, such as natural killer cells. The gel droplets include the presence of extracellular matrix components, which alter cell functions including viability and mobility. The system allows cells co-incorporated into 3D gel droplets to be observed in real-time using fluorescence microscopy, with fluorescence-based sorting of droplets for recovery of individual cells based on their function. High-quality RNA can be extracted from the sorted droplets for transcription profiling.

The invention discloses a complete virus particle detection quantification system and method based on droplet microfluidics, and belongs to the field of micro-nano technology, the system comprises a single virus adsorption microsphere and two subsystems; the single virus adsorption microspheres are coupled with an antibody through a streptavidin chemical bond, and single viruses are adsorbed by virtue of antigen-antibody specific binding; the single virus nucleic acid detection subsystem wraps microsphere liquid drops with viruses, water-phase liquid containing microsphere-virus samples and a fluorescent probe reagent are subjected to RT-PCR amplification, single virus nucleic acid is detected in the liquid drops, and fluorescent probes are used for calibrating and comparing fluorescence intensity. And the high-throughput liquid drop screening subsystem analyzes the size of the liquid drop and the intensity of the fluorescence signal, identifies the liquid drop containing the single virus, and accurately counts the fluorescence liquid drops, so that quantitative analysis on the single virus level is realized. Complete virus particles are identified by adopting a method of combining nucleic acid detection and protein detection, and high-sensitivity detection and absolute quantitative analysis on a single virus level are realized by adopting a droplet microfluidic technology.

The invention discloses a preparation method of an exosome-enriched silkworm chrysalis chitosan magnetic microsphere and application of the exosome-enriched silkworm chrysalis chitosan magnetic microsphere in treatment of intrauterine adhesion, the method is based on a droplet microfluidic technology, chitosan suspension of magnetic nanoparticles is prepared and is uniformly distributed through high-speed oscillation, high-voltage direct current is used for realizing dispersion of droplets, and the magnetic microspheres are prepared. And injecting the dispersed liquid drops into an alkaline solution from a proper height, and curing into balls. A large number of in-vivo and in-vitro experiments prove that the synthesized silkworm chrysalis chitosan magnetic microspheres have good biocompatibility and directional movement capacity, meanwhile, the drug loading and releasing performance of the microspheres is stable, the safety meets the in-vivo injection requirement, and a new thought is provided for treatment of intrauterine adhesion.

The application relates to an integrated multifunctional integrated droplet microfluidic chip and application thereof, the integrated multifunctional integrated droplet microfluidic chip comprising a droplet generation area (1), a biochemical reaction incubation area (9) and a droplet sorting area (10). The application realizes the integrated function of single B cell droplet generation, incubation and sorting, meets the high-throughput demand, effectively reduces droplet fusion, is simple to operate and controllable in cost. The application also has important scientific application value in the fields of stem cell screening, immunotherapy CAR-T cell screening, microbial strain screening, organoid construction and other biological synthesis, disease diagnosis and microbial research.

The invention provides a liquid drop micro-fluidic chip based on a micro-filtration array. The micro-filtration array structure is highly integrated in the water phase sample introduction area of the chip, synchronous filtration of cell suspension in the inflow process is achieved, newly formed cell blocks or fragments can be effectively intercepted and prevented from entering the liquid drop generation area, and therefore the liquid drop wrapping quality is guaranteed from the source. In an actual test, the droplet micro-fluidic chip has an efficient filtering capability, supports the droplet generation frequency of about 1000 Hz, has been verified in a 293T cell suspension system, has good stability and repeatability, and can be widely applied to single cell sequencing, cell screening and other high-throughput single cell analysis scenes.

This application discloses a method for screening single B cells. The method provided in this application uses magnetic beads coated with IL-6 antigen, combined with fluorescently labeled secondary antibody, and employs droplet microfluidic technology to rapidly sort B cells. The obtained IL-6 positive B cells are then directly cultured, and the generated antibodies are subjected to ELISA and IL-6 antigen-antibody affinity detection to obtain the target cell line. The entire process is greatly simplified and the workload is significantly reduced.

This invention provides a label-free live cell screening system and method based on droplet microfluidics. The system includes: a microfluidic chip with fluorescence signal monitoring sites, imaging sites, and sorting sites; a fluorescence detection unit configured to excite and acquire fluorescence signals at the fluorescence signal monitoring sites; an imaging unit configured to acquire images of droplets at the imaging sites; a sorting unit configured to sort droplets encapsulating target cells at the sorting sites; and a signal processor that, when a droplet is detected passing through the fluorescence signal monitoring site based on the fluorescence signal, triggers the imaging unit to acquire images of the droplet at the imaging site; and when the signal processor determines, based on image analysis, that the droplet encapsulates a target cell, triggers the sorting unit to sort the droplet encapsulating the target cell at the sorting site. This invention utilizes fluorescence signals to monitor droplets, improving monitoring efficiency and accuracy, thereby significantly increasing the precision and throughput of droplet sorting.

The invention discloses a droplet microfluidic detection system for detecting pathogenic bacteria based on a deoxyribozyme probe, which belongs to the technical field of microbiological detection, and mainly comprises an automatic sample introduction device, a microfluidic droplet generation chip, a capillary tube-laser induced fluorescence detector and a signal acquisition and analysis device, the pathogen detection process comprises the following steps: respectively and automatically injecting an actual water sample, a deoxyribozyme probe, liquid drop oil, a pathogen lysis solution and a reaction buffer solution into the microfluidic liquid drop generation chip by the automatic sample injection device, stably generating liquid drops, and detecting the liquid drops through the capillary tube-laser induced fluorescence detector, and the signal acquisition and analysis device acquires and analyzes the fluorescence signal. The method has the advantages of high detection flux, high stability, high sensitivity, rapidness and the like, and can be applied to the field of environmental microbiological detection.

This invention provides a peptide synthesis system and method based on a digital droplet microfluidic chip. The peptide synthesis system includes a fluid transport mechanism, a digital droplet microfluidic chip, and a control mechanism. The digital droplet microfluidic chip includes a first substrate and a second substrate disposed opposite each other, with a droplet-containing space formed between the first and second substrates. The first and second substrates form a peptide synthesis electrode array composed of a plurality of peptide synthesis electrode units. The control mechanism controls the fluid transport mechanism to drive the raw material droplets to each peptide synthesis region according to a planned droplet movement path to react with magnetic bead droplets for peptide synthesis. The peptide synthesis region is composed of one or more peptide synthesis electrode units. This invention utilizes microliter-level droplet synthesis for peptide synthesis. Due to the small volume of the microdroplets, the mass transfer rate within the droplets is very fast, shortening the time required for each step in the synthesis process.

The invention belongs to the technical field of biology, and particularly relates to a single cell ATAC sequencing method based on droplet microfluidics. The reagent combination provided by the invention is characterized in that a fragmented product with different captured sequences at two ends is obtained by cutting an open region of chromatin through a transposition complex with different linker sequences, and due to the different captured sequences, the loss of the fragmented product of a sample is reduced; according to the present invention, the nucleic acid molecule coupled on the carrier can capture the fragmented product through the capture sequence, the cell label contained in the nucleic acid molecule can further distinguish different cells or cell nucleuses so as to achieve the single cell level chromatin open region analysis, and the reagent can be used for the microfluidic system so as to achieve the high-throughput detection.

The invention discloses chip detachable press-fit liquid drop microfluidic production equipment. The production equipment comprises a chip press-fit module, a continuous liquid inlet module and a microsphere detection module, the chip press-fit module comprises a chip assembly, a lever and a press-fit air cylinder, the chip assembly comprises a cover plate, a micro-fluidic chip and a bottom plate which are stacked from top to bottom, a channel is formed in the micro-fluidic chip, one end of the lever is connected with the press-fit air cylinder, and the other end of the lever applies pressure to the chip assembly through a pressing assembly so as to seal the micro-fluidic chip; the continuous liquid inlet module comprises a liquid inlet tank and a liquid supplementing tank, the liquid inlet tank is connected with a liquid inlet of the chip assembly through a liquid outlet pipeline, the liquid supplementing tank is connected with the liquid inlet tank through a liquid supplementing pipeline, and a check valve is arranged on the liquid supplementing pipeline; and the microsphere detection module is used for detecting the particle size of microspheres contained in the solution discharged from the liquid outlet of the chip assembly. According to the equipment, the micro-fluidic chip can be detached, the continuous production of liquid drop micro-fluidic can be realized, and the liquid drop on-line detection function is realized at the same time.