99 results about "Droplet microfluidics" patented technology

The invention belongs to the technical field of microfluidics, and particularly relates to a liquid separation-convergence integrated liquid drop micro-fluidic chip and a liquid drop preparation method.The liquid separation-convergence integrated liquid drop micro-fluidic chip is characterized in that a liquid drop generation unit is arranged at the communication position of a branch splitting channel and a convergence channel which is correspondingly communicated with the branch splitting channel; and under the action of continuous phase shearing force, the dispersed phase solution is sheared off by the continuous phase solution, so that liquid drops are formed. The droplet generation units are symmetrically arranged along the circumference of the channel, the distance from each unit to the center is equal, and a continuous phase solution and a dispersed phase solution encountered at each droplet generation unit are almost equivalent, so that the sizes of the generated droplets are consistent, the droplets with basically consistent sizes are obtained at a collection port, and high-flux preparation of microspheres with uniform sizes is realized.

The invention relates to the technical field of droplet digital nucleic acid detection and biological materials, in particular to microgel based on acrylic anhydride gelatin and nucleic acid to be detected as well as preparation and application of the microgel. The preparation method comprises the following steps: firstly, dissolving a photoinitiator and acrylic anhydride gelatin, and uniformly mixing to obtain a pre-polymerized solution; uniformly mixing the pre-polymerized solution with a to-be-detected nucleic acid mixed solution to obtain a dispersion phase; oil incompatible with the dispersed phase is used as a continuous phase; building a constant-temperature device, and enabling the dispersed phase to form liquid drops by using a liquid drop microfluidic method; and finally, performing amplification treatment on the liquid drops, and then performing photo-initiation crosslinking to obtain the microgel based on the acrylic anhydride gelatin and the nucleic acid to be detected. The prepared microgel based on the acrylic anhydride gelatin and the nucleic acid to be detected is further applied to detection of target nucleic acid.

The invention discloses an EV membrane protein single molecule detection method based on droplet microfluidics and application thereof. The method comprises the following steps: firstly, constructing an Ab-oligo compound which is formed by sequentially connecting an antibody, Linker, Linker connection DNA, RNA capable of being specifically cut by RNA enzyme and template DNA for starting signal amplification; then capturing the extracellular vesicles in the plasma by using the CD9 / CD63 / CD81 functionalized magnetic beads; the method comprises the following steps: adding an Ab-oligo compound and a sealing buffer solution into extracellular vesicles, incubating, removing the uncombined Ab-oligo compound, adding RNA enzyme for incubating and enzyme digestion, collecting template DNA for starting signal amplification, and performing microdroplet digital PCR analysis to realize single molecule analysis of EV membrane protein. According to the invention, the one-to-one correspondence relationship between the antibody and oligonucleotide is established through Linker for the first time, single protein information is converted into single nucleic acid chain information, and EV membrane protein single molecule detection is realized. The method is simple to operate, can qualitatively and quantitatively detect the single EV membrane protein, and is beneficial to further research on the relationship between the EV membrane protein and diseases.

The invention belongs to the technical field of cosmetics, and particularly relates to a water-in-oil-in-water three-phase coated microbead as well as a preparation method and application thereof. The water-in-oil-in-water three-phase coated microbead is of a spherical structure and sequentially comprises an outer water phase, an inner oil phase and an inner water phase from outside to inside, the outer water phase comprises sodium alginate, agarose, cyclodextrin, glycerin and other components, the inner oil phase comprises caprylic / capric triglyceride, grape seed oil, olive squalane, tocopherol and other components, and the inner water phase comprises sodium alginate, agarose, cyclodextrin, glycerin and other components. And the inner water phase comprises ampelopsis grossedentata extract, astragalus membranacus root extract and other components. According to the invention, an easily oxidized and unstable active matter is fully and firmly protected by adopting a droplet microfluidic coating technology, so that the active matter is free from external stimulation, active ingredients with different effects play multiple roles, and the composition has good anti-inflammatory, soothing and oil-controlling effects and can be applied to the field of cosmetics.

The invention belongs to the technical field of microfluidics, and particularly relates to an integrated liquid drop micro-fluidic chip based on 3D printing and a liquid drop preparation method. Comprising a continuous phase channel unit and a dispersed phase channel unit, the continuous phase channel unit comprises a main channel unit, and a plurality of main split channels are communicated in the circumferential direction around an outlet of the main channel unit; the dispersed phase channel unit comprises a branch channel unit, a plurality of branch splitting channels are circumferentially communicated around an outlet of the branch channel unit, a droplet control structure is arranged at the communication part of the main splitting channel and the outlet of the branch splitting channel, and a droplet generation channel is arranged at the communication part of the outlet of the branch splitting channel and the outlet of the main splitting channel; an outlet of each liquid drop generation channel is communicated with a collection opening; the multiple collection openings communicate with the amplification section structure, and an outlet of the amplification section structure communicates with a total collection opening. The three-dimensional space is fully utilized, high-flux and uniform preparation of the liquid drops can be achieved, and the method is suitable for large-scale production of the liquid drops.

The invention discloses a hydrogel microsphere sorting method, an artificial organ preparation method, a system and a medium. The method comprises the following steps: acquiring a hydrogel microsphere image in the micro-fluidic chip; analyzing the hydrogel microsphere image through a neural network to obtain an analysis result; based on the analysis result, hydrogel microspheres wrapping the target cells are sorted out; the neural network comprises a feature extraction layer and a multi-scale joint output head, and the hydrogel microsphere image is processed by the multi-scale joint output head after being convolved by the feature extraction layer to obtain an analysis result. According to the method, the hydrogel microspheres wrapped with the target cells are accurately identified and sorted by using a droplet microfluidic technology, a standardized artificial organ can be prepared based on the sorted hydrogel microspheres, and the functional maturity and reliability of the artificial organ are remarkably improved.

The utility model provides a digital droplet micro-fluidic chip system and relates to the technical field of microbiological detection. The digital liquid drop micro-fluidic chip system comprises a micro-fluidic liquid drop generation chip, a micro-fluidic liquid drop fusion chip and a micro-fluidic liquid drop reaction chip. The microfluidic liquid drop generation chip comprises n liquid drop generation units, wherein each liquid drop generation unit comprises a first dispersed phase inlet, a second dispersed phase inlet, a dispersed phase mixing channel and a liquid drop generation area; the dispersed phase mixing channel is of a snakelike structure, a broken line structure or a spiral structure; the liquid drop generation area comprises a cross intersection, and the cross intersection is of a closing-up structure which is in a trapezoid shape. The digital droplet micro-fluidic chip system disclosed by the utility model has the advantages of high detection flux, high stability, high sensitivity, rapidness, low cost and the like, is suitable for simultaneously detecting various bacteria in an unknown sample, and can be applied to the fields of environmental microorganism detection, food-borne pathogenic bacterium detection, clinical pathogenic bacterium diagnosis and the like.

The invention provides a room-temperature high-chemical-yield ultrasonic enhanced droplet microfluidic synthesis method of perovskite quantum dots. The method comprises the following steps: manufacturing a droplet microfluidic reaction chip with a two-section series double-T-shaped structure, and coupling the droplet microfluidic reaction chip with an ultrasonic vibrator to form a reaction system; the precursor solution, the ligand solution and the acetonitrile solution are injected into a droplet microfluidic reaction chip in proportion, and an ultrasonic vibrator is started to carry out ultrasonic enhancement on the reaction solution. And centrifugally drying the product to obtain the perovskite quantum dots. Compared with the prior art, the method has the following beneficial effects: 1, mass transfer between two phases is accelerated through ultrasonic treatment, the reaction process is enhanced, and the chemical yield and efficiency of the quantum dots are improved; 2, the size of the quantum dots can be regulated and controlled by adjusting the flow rate ratio and the ultrasonic power; 3, the specific surface area of reactants can be increased by utilizing the constructed reaction system, and the reaction conditions can be accurately controlled; and 4, the reaction system is high in automation compatibility degree and suitable for distributed continuous production.

The present application relates to the technical field of microfluidic chip, and disclose a centrifugal microfluidic chip for generating different size droplets simultaneously, including a liquid distribution channel for uniformly distributing sample to multiple droplet generation units, the design of a quantitative pool ensures the fixed volume of liquid and the consistency of the number of droplet generation, the chip contains a sample inlet and two continuous phase reagent inlets, the sample is precisely distributed to the quantitative pool by centrifugal force and enters the droplet generation units respectively, the first droplet generation unit is used for generating large size droplets with a diameter of 100-200 mu m, and the second droplet generation unit is used for generating small size droplets with a diameter of 10-100 mu m, the microfluidic chip can realize the preparation of ultrahigh throughput droplets, the generated droplets are guided to the collection area by centrifugal force to form a regular single-layer arrangement, the chip has wide application in the fields of biomedical research, material science, chemical synthesis and environmental monitoring, especially in the preparation of fluorescent microspheres, which can improve the experimental efficiency and result consistency.

The invention discloses an electrically-driven integrated liquid drop microfluidic device, relates to the technical field of microfluidics, and is used for solving the technical problems that most of the existing control modes are limited to plane control and cannot adapt to unstructured working environments in a deep cavity and a biochemical analyzer. The electrically-driven integrated liquid drop microfluidic device comprises a supporting plate, a liquid collecting plate, a detection platform, a displacement mechanism and a liquid transferring mechanism, the supporting plate is provided with a first surface and a second surface which are oppositely arranged; the supporting plate is arranged on the fixed platform through the first surface; the liquid collecting plate is arranged on the supporting plate through the second surface; the detection platform is arranged at any end of the liquid collection plate; the displacement mechanism is arranged to move in the horizontal and / or vertical direction relative to the liquid collecting plate; the pipetting mechanism is arranged on the displacement mechanism and is used for moving the liquid drops or the liquid marbles from the liquid collection area to the detection platform through dielectrophoretic force.

The application discloses a kind of based on digital droplet microfluidic multi-sample flux multi-index detection device and application thereof, belong to electromechanical device, microfluidic chip and immunoprotein analysis field.The device includes droplet microfluidic equipment and immunoprotein analysis chip component, droplet microfluidic equipment is used to drive multiple droplets, mainly divided into three major systems: power supply system, computer operating system and chip clamp system, immunoprotein analysis chip component includes biological analysis substrate and microarray chip.The present application drives micro liquid sample by digital droplet microfluidic technology, realizes the multi-index detection of automated immunoprotein analysis chip.The detection device of the present application has a series of advantages such as rapidity, independence, low consumption, automation, function integration and multi-index detection, and is expected to become the most fruitful rapid diagnostic device for improving tumor diagnosis positive rate.

The application provides a preparation method of organoids based on a single cell chip, which comprises microfluidic chip preparation, a two-water-phase solution preparation, a single cell suspension preparation, microfluidic manipulation, single cell-loaded microgel generation and organoid construction and the like. The application takes a microfluidic chip integrated with a normally closed pneumatic pump valve as a technical platform, takes a single cell suspension pre-incubated with a crosslinking agent as a chemical reaction core, takes a water-in-water droplet as a forming template, and realizes efficient in-situ loading of single dispersed seed cells in a micro hydrogel carrier through a one-step method, and simply removes empty droplets without cells in a subsequent transfer step. The single cells in the microgel can be cultured, expanded and self-organized to form required organoids. The application improves the consistency of the starting point of the organoids, has the high-throughput characteristics of the droplet microfluidic technology, realizes efficient and highly reproducible in-vitro construction of the organoids, and can play a great role in the fields of basic research and transformation application related to the organoids.

The invention relates to the technical field of polymer microparticle preparation, in particular to a special-shaped polymer microparticle preparation method which comprises the following steps: taking a polymer-containing solution as an inner oil phase and a polyvinyl alcohol aqueous solution as an outer water phase, generating micro-droplets by using a droplet micro-fluidic chip, and standing the micro-droplets under a constant-temperature condition to volatilize a solvent; and by regulating and controlling the components and proportion of the polymer and the concentration and constant temperature of the polyvinyl alcohol aqueous solution, the micro-droplets are induced to generate controllable phase separation or interface self-assembly, and are finally cured into the special-shaped polymer microparticles. According to the invention, controllable preparation of spherical, dumbbell-shaped, raspberry-shaped and other polymorphic microparticles is realized through a droplet microfluidic technology and a solvent volatilization coordinated regulation strategy. The method has the characteristics of universality, high repeatability and simple and convenient process, can adapt to various polymer systems, and meets the diversified requirements of the fields of biomedicine, catalysis, functional materials and the like on microparticles with complex shapes.

Single-cell methods are provided for investigating DNA and RNA interactions with regulatory proteins and evaluating the cytotoxicity of immune cells towards target cells, such as tumor cells. The methods utilize droplet microfluidics and imaging-based technology to facilitate the isolation and analysis of protein-bound nucleic acids from individual immune cells or apoptotic tumor cells. The methods utilize fluorescence-activated droplet sorting and are capable of isolating and characterizing mitochondrial DNA from cytotoxic immune cells.

The invention discloses an intelligent preparation and control method for nano metal powder driven by a droplet microfluidic technology, relates to the technical field of metal powder processing, and aims to accurately control particle distribution, calculate particle size distribution uniformity Dcv and dispersion stability coefficient Dsf and optimize a shear flow field through a droplet interface dynamic model in combination with a dynamic light scattering and adaptive optimization algorithm. The particle size uniformity is ensured, the powder stacking density and the conductive stability are improved, and the problem that particle size distribution is difficult to accurately control in an existing method is solved; a high-sensitivity sensor is adopted to monitor environmental interference, Fourier transform analysis and Gaussian process regression reinforcement learning are combined to optimize temperature and flow velocity, by-products and pollutants are reduced, powder purity is ensured, long-term stability of electronic devices is improved, and resistance drift or local overheating is avoided. And aiming at the agglomeration problem, an agglomeration trend value Pag is calculated and graded evaluation is carried out, so that particle agglomeration is effectively inhibited, and the stability and reliability of the conductive material are improved.

The invention discloses a method and a device for preparing liquid drops by using a liquid drop generating device in the technical field of liquid drop microfluidics, and the device comprises a liquid drop receiving tank for containing continuous phase liquid, a liquid drop generating mechanism for producing the liquid drops and a micro-vibration mechanism for providing a vibration source, wherein the vibration output direction of the piezoelectric actuator is perpendicular to or approximately perpendicular to the length direction of the pointed-end pipe, so that the continuous-phase liquid near the nozzle can conveniently form an acoustic vortex effect to promote the formation of liquid drops. The method for preparing the liquid drops comprises the following steps: S1, respectively preparing a dispersed phase liquid and a continuous phase liquid; s2, extending a nozzle to a depth h below the liquid level of the continuous-phase liquid; s3, working parameters of the piezoelectric actuator are set, the working parameters comprise the voltage U and the sine wave frequency f1, the voltage U ranges from 20 V to 120 V, and the sine wave frequency f1 ranges from 2 kHz to 20 kHz. By triggering the local acoustic vortex effect in the continuous phase, the formation of the liquid drops is promoted, and the purposes of reducing the particle size variation of the liquid drops and improving the production efficiency of the liquid drops are achieved.

The invention relates to the technical field of micro-nano sensing and detection, in particular to a temperature sensing method based on a micro-nano robot. The invention discloses a temperature detection method based on a micro-nano robot. The temperature detection method comprises the following steps: step 1, preparing a temperature sensitive material by using copper tetraamminesulfate particles and resin; 2, preparing a micro-nano robot with a temperature detection function by adopting a droplet microfluidic technology and a physical vapor deposition technology based on the temperature sensitive material in the step 1; 3, extracting color features by using the micro-nano robot with the temperature detection function obtained in the step 2, and building a temperature detection regression prediction model based on the color features; and 4, on the basis of the temperature regression prediction model obtained in the step 3, the micro-nano robot with the temperature detection function is used for carrying out temperature detection on the micro gap, and the average value of the detection results of the multiple micro-nano robots is taken as the final detection temperature. The problem that an existing temperature detection method in the industrial field is limited is solved.

Provided are a single-cell chromatin accessibility and transcriptome combined sequencing method and application thereof. The method comprises: treating a cell nucleus using a transposase to obtain gDNA of a chromatin open region connected with a first specific tag sequence; treating the cell nucleus using a transcriptome capture sequence to obtain cDNA connected with a second specific tag sequence; generating a droplet based on a droplet microfluidic to seal the cell nucleus and a first microbead in the droplet, wherein the gDNA and the cDNA are captured by the first microbead, and wherein more than one cell nucleus is sealed in the droplet; and sequencing the gDNA and the cDNA to obtain combined information of single-cell chromatin accessibility and transcriptome according to the first specific tag sequence and the second specific tag.

The present invention relates to the field of droplet microfluidics, particularly relates to a droplet sorting chip. The droplet sorting chip disclosed herein is provided with a cavity area, which can accommodate impurities such as fibers entering the droplet sorting chip, can effectively prevent clogging and collision and fusion of droplets caused by impurities, and ensure smooth droplet sorting.

The application discloses a polar microorganism separation and culture method based on a droplet microfluidic technology. The method comprises the following steps: obtaining a polar sample and performing oscillation extraction at low temperature to obtain a sample suspension; determining a plate cultivable rate R through a solid plate culture method; based on the R and a preset target number of cultivable cells N, calculating a target total number of cells T required by a droplet microfluidic experiment, and preparing a sample with a corresponding cell concentration for machine loading; generating single-cell-wrapped microdroplets through a droplet microfluidic system for the sample for machine loading, and performing long-term culture at low temperature; and screening positive droplets and purifying strains after the culture. Through the cultivable rate feedback, the sample concentration for the microfluidic loading is precisely optimized, the separation efficiency of the polar difficult-to-culture microorganism is significantly improved, the inter-species competition inhibition of the traditional plate is broken, and the diversity and novel group proportion of the obtained strains are greatly improved.

The application discloses a droplet microfluidic sorting method, a sorting system, an electronic device and a storage medium. The droplet microfluidic sorting method comprises the following steps: acquiring a first time point of positive droplet trigger sorting in a microfluidic device; determining a second time point of the positive droplet reaching an outlet of a collection channel according to the first time point and a time length of the positive droplet passing through the collection channel to reach the outlet of the collection channel; the outlet of the collection channel is used for forming an oil droplet; and comparing an oil droplet formation time point closest to the first time point with the second time point to determine a target oil droplet containing the positive droplet. The droplet microfluidic sorting method realizes the automation of sorting a single target droplet, further sorts the target liquid into a droplet containing a single target bacterium or cell, avoids the loss of cells in the demulsification process or the phenomenon that target cells do not exist in part of the well plates or multiple target cells exist in part of the well plates, and thus the efficiency of target sequence acquisition is improved.

The application provides a method for detecting secretion expression of a target protein, which comprises the following steps: fusing the target protein with a fluorescence-enhanced nanobody to obtain a fusion protein; adding the obtained fusion protein into a system containing a fluorescent protein and a fluorescence-inhibited nanobody to perform a reaction; and measuring the fluorescence intensity of the system after the reaction is completed, and calculating the secretion expression amount of the target protein according to the change amount of the fluorescence intensity. In the application, the GBP1 is fused with the target protein, and the expression interference of the GBP1 on the target protein is reduced by adding a connecting peptide, so that the secretion expression amount of the target protein is determined. The method of the application can detect a wide concentration range of the target protein, has a high signal response multiple, and can be applied to a shake flask, a well plate and a droplet microfluidic screening system.

The invention discloses a droplet micro-fluidic device for cell co-culture and a use method, belongs to the field of micro-fluidic analysis, and particularly relates to a droplet micro-fluidic device which comprises a chip main body material, an oily liquid substance covering the surface of the chip main body material, and a vertical porous membrane material arranged in the oily liquid substance. The porous membrane material comprises at least one of a polycarbonate porous membrane, a PVDF (Polyvinylidene Fluoride) porous membrane and a porous composite membrane, and the porosity of the porous membrane material is more than 1%. The droplet microfluidic device has the advantages of high flux, low consumption, multi-step operation, direct observation and the like, and has strong compatibility with a classical biochemical mechanism analysis method. The liquid drops are separated by the porous membrane material and are connected with each other, and the liquid drops on the two sides can be used as a cell, organoid or tissue culture chamber and also can be used as a liquid storage tank for sample adding. The liquid drops are used as a culture chamber, so that co-culture of cells can be realized, and support is provided for cell co-culture, drug screening and cell microenvironment simulation.

1. Name of the product in this design: Main body of a droplet microfluidic cell sorter. 2. Application of this design: The main body of the droplet microfluidic cell sorter is used for sorting and screening cells. 3. The key design features of this product are its main shape and the pattern of its main view. 4. The image or photograph that best illustrates the design's key points: a 3D model.

The present application relates to the field of droplet microfluidics, and more particularly to a droplet reading chip and a method of using the same. The disclosed droplet reading chip comprises a microcavity for tiling droplets and a pipette for pipetting droplets into the microcavity. The chip can tile a large number of droplets at the same time, meeting the requirement of high-throughput detection of droplets. In addition, the chip can keep the droplets in a relatively closed space during detection, reducing the risk of contamination.

The invention belongs to the technical field of biology, and particularly relates to high-throughput multi-mode index marking and sequencing detection on single-cell or subcellular nucleic acid substances. According to the invention, a microbead-coupled particle of a PCR primer containing a bar code is designed, the microbead-coupled particle can be delivered to a plurality of different reaction systems in a single reaction by means of droplet microfluidics and the like, and a specific bar code of the reaction system is added to a target nucleic acid molecule in the reaction system in a high-flux parallel manner; the single cell transcriptome or multi-omics sequencing database building is completed. The method disclosed by the invention has the advantages of large cell flux, simplicity and convenience in operation, low price, low pseudosingle cell rate, low no-load rate of a micro-reaction system, high data quality and strong applicability.

The application provides a method for preparing hollow non-spherical micro-nanoparticles based on a coaxial micro-reactor, and belongs to the field of droplet microfluidics and polymer materials. A three-channel coaxial micro-reactor is designed to generate a water-in-oil-in-water (W / O / W) double emulsion. The innermost phase is an alkaline electrolyte aqueous solution, the intermediate oil phase is a polymer solution, and the outermost phase is a surfactant aqueous solution. With the volatilization of the intermediate phase solvent, the double emulsion droplets form hollow non-spherical micro-nanoparticles. The double emulsion is placed in a collection liquid with different electrolyte concentrations, water molecules in the collection liquid penetrate into the innermost phase under the action of osmotic pressure, increase the size of the inner droplets of the double emulsion, and then change the morphology of the outer droplets, so that the non-spherical structure required is presented, and the size of the hollow micro-nanoparticles is controllable. The coaxial micro-channel reactor used in the application adopts a flow focusing structure, which is used for controllable generation of double emulsion to prepare multi-chamber hollow non-spherical micro-nanoparticles.

The application discloses ecdysterone-low molecular weight fructan composite microspheres with fat-reducing and muscle-increasing functions, and a preparation method and application thereof, and belongs to the technical field of biological medicine and functional food. The composite microspheres have a core-shell structure, the inner core is a calcium alginate gel, the outer shell is chitosan, the inner core co-encapsulates ecdysterone and low molecular weight fructan, and the mass ratio of the two is 0.8-1.2:1. The preparation adopts a liquid drop microfluidic technology combined with an internal gelation method to form the inner core, and adopts a post-crosslinking method to coat the outer shell. The composite microspheres are regular in shape, uniform in particle size, high in encapsulation rate, good in stability, excellent in pH response slow-release and anti-degradation characteristics in the gastrointestinal tract. In-vivo pharmacological experiments prove that the composite microspheres can reduce the body weight and body fat rate of obese mice, increase the lean meat content and muscle strength, realize synergistic effects of fat reduction and muscle increase, and can be used for preparing drugs or functional food for improving obesity-related muscle loss, low-muscle obesity and related metabolic syndrome.

Microfluidics technology incorporating 3D gel droplets is provided for analysis of tumor cells and their interactions with immune cells, such as natural killer cells. The gel droplets include the presence of extracellular matrix components, which alter cell functions including viability and mobility. The system allows cells co-incorporated into 3D gel droplets to be observed in real-time using fluorescence microscopy, with fluorescence-based sorting of droplets for recovery of individual cells based on their function. High-quality RNA can be extracted from the sorted droplets for transcription profiling.

The invention discloses a complete virus particle detection quantification system and method based on droplet microfluidics, and belongs to the field of micro-nano technology, the system comprises a single virus adsorption microsphere and two subsystems; the single virus adsorption microspheres are coupled with an antibody through a streptavidin chemical bond, and single viruses are adsorbed by virtue of antigen-antibody specific binding; the single virus nucleic acid detection subsystem wraps microsphere liquid drops with viruses, water-phase liquid containing microsphere-virus samples and a fluorescent probe reagent are subjected to RT-PCR amplification, single virus nucleic acid is detected in the liquid drops, and fluorescent probes are used for calibrating and comparing fluorescence intensity. And the high-throughput liquid drop screening subsystem analyzes the size of the liquid drop and the intensity of the fluorescence signal, identifies the liquid drop containing the single virus, and accurately counts the fluorescence liquid drops, so that quantitative analysis on the single virus level is realized. Complete virus particles are identified by adopting a method of combining nucleic acid detection and protein detection, and high-sensitivity detection and absolute quantitative analysis on a single virus level are realized by adopting a droplet microfluidic technology.