141 results about "Droplet microfluidics" patented technology

The invention discloses a single-cell metabolite spectrum quantitative analysis platform and method based on droplet microfluidics, and relates to the technical field of mass spectrometry. The platform comprises a fluid pumping module, a cell oscillation module, a microfluidic mixed liquid drop generation module, a microfluidic mixed liquid drop reinjection and separation module and a mass spectrometry module. The fluid pumping module comprises a high-pressure nitrogen cylinder and a gas pressure driver; the cell oscillation module comprises a cell oscillator; the micro-fluidic mixed droplet generation module comprises a micro-fluidic droplet generation chip, a cell lysis solution, an internal standard, an oil phase, a cell suspension and a droplet collection and incubation centrifugal tube; the micro-fluidic mixed liquid drop reinjection and separation module comprises a micro-fluidic liquid drop reinjection and separation chip; and the mass spectrometry module comprises an electrospray needle and a mass spectrometer. According to the method, the single cell is captured and the internal standard substance is introduced at the same time by using droplet microfluidics, and mass spectrometry is carried out, so that high-flux single cell encapsulation and accurate quantification of metabolites are realized.

The invention belongs to the technical field of microfluidics, and particularly relates to a liquid separation-convergence integrated liquid drop micro-fluidic chip and a liquid drop preparation method.The liquid separation-convergence integrated liquid drop micro-fluidic chip is characterized in that a liquid drop generation unit is arranged at the communication position of a branch splitting channel and a convergence channel which is correspondingly communicated with the branch splitting channel; and under the action of continuous phase shearing force, the dispersed phase solution is sheared off by the continuous phase solution, so that liquid drops are formed. The droplet generation units are symmetrically arranged along the circumference of the channel, the distance from each unit to the center is equal, and a continuous phase solution and a dispersed phase solution encountered at each droplet generation unit are almost equivalent, so that the sizes of the generated droplets are consistent, the droplets with basically consistent sizes are obtained at a collection port, and high-flux preparation of microspheres with uniform sizes is realized.

The invention discloses a magnetic control liquid drop micro-fluidic chip, device and method for chemiluminescence immunoassay, and belongs to the technical field of chemiluminescence immunoassay, the micro-fluidic chip comprises: a chip main body, which comprises a plurality of vertically through separation chambers for loading a reagent and serving as a reaction container, if the chip has the function of separating plasma from the whole blood sample, the first cavity adopts a bottom closed structure, and no gap is formed in the separation position between the first cavity and the second cavity; the top thin film and the bottom thin film are used for sealing the separated cavity of the chip main body; and the top gland and the bottom gland are matched with the chip main body in structural shape and are used for firmly attaching the two layers of films to the chip main body. According to the invention, the whole process integration of sample pretreatment, reagent mixing, reaction incubation and chemiluminescence detection can be realized, and a chemiluminescence immunoassay solution which is efficient, low in cost and easy to operate is provided through chip design, device integration and method optimization.

The invention relates to the technical field of droplet digital nucleic acid detection and biological materials, in particular to microgel based on acrylic anhydride gelatin and nucleic acid to be detected as well as preparation and application of the microgel. The preparation method comprises the following steps: firstly, dissolving a photoinitiator and acrylic anhydride gelatin, and uniformly mixing to obtain a pre-polymerized solution; uniformly mixing the pre-polymerized solution with a to-be-detected nucleic acid mixed solution to obtain a dispersion phase; oil incompatible with the dispersed phase is used as a continuous phase; building a constant-temperature device, and enabling the dispersed phase to form liquid drops by using a liquid drop microfluidic method; and finally, performing amplification treatment on the liquid drops, and then performing photo-initiation crosslinking to obtain the microgel based on the acrylic anhydride gelatin and the nucleic acid to be detected. The prepared microgel based on the acrylic anhydride gelatin and the nucleic acid to be detected is further applied to detection of target nucleic acid.

The invention discloses an EV membrane protein single molecule detection method based on droplet microfluidics and application thereof. The method comprises the following steps: firstly, constructing an Ab-oligo compound which is formed by sequentially connecting an antibody, Linker, Linker connection DNA, RNA capable of being specifically cut by RNA enzyme and template DNA for starting signal amplification; then capturing the extracellular vesicles in the plasma by using the CD9 / CD63 / CD81 functionalized magnetic beads; the method comprises the following steps: adding an Ab-oligo compound and a sealing buffer solution into extracellular vesicles, incubating, removing the uncombined Ab-oligo compound, adding RNA enzyme for incubating and enzyme digestion, collecting template DNA for starting signal amplification, and performing microdroplet digital PCR analysis to realize single molecule analysis of EV membrane protein. According to the invention, the one-to-one correspondence relationship between the antibody and oligonucleotide is established through Linker for the first time, single protein information is converted into single nucleic acid chain information, and EV membrane protein single molecule detection is realized. The method is simple to operate, can qualitatively and quantitatively detect the single EV membrane protein, and is beneficial to further research on the relationship between the EV membrane protein and diseases.

The present invention provides a real-time flow cytometric analyzer of single-cell dynamics combining droplet microfluidics and multimodal optical microscopy, and belongs to the field of cell biology experimental devices. The analyzer comprises a cell / signal loading & acquisition and control system, a high-throughput microfluidic single-cell manipulation chip, a multi-channel optical microscopy and multimodal signal acquisition and control system, and a data processing and analysis system. Based on droplet-based microfluidic chip technology, multimodal optical microscopy technology, single-cell dynamics theoretical modeling and analysis methods, and artificial intelligence technology, the integrated detection of spatiotemporal information of single-cell dynamics can be realized. The analyzer provides a new rapid and accurate single-cell dynamics analysis platform for exploring the occurrence and development mechanism of major human diseases, disease diagnosis, cell sorting and drug screening, etc.

The invention belongs to the technical field of cosmetics, and particularly relates to a water-in-oil-in-water three-phase coated microbead as well as a preparation method and application thereof. The water-in-oil-in-water three-phase coated microbead is of a spherical structure and sequentially comprises an outer water phase, an inner oil phase and an inner water phase from outside to inside, the outer water phase comprises sodium alginate, agarose, cyclodextrin, glycerin and other components, the inner oil phase comprises caprylic / capric triglyceride, grape seed oil, olive squalane, tocopherol and other components, and the inner water phase comprises sodium alginate, agarose, cyclodextrin, glycerin and other components. And the inner water phase comprises ampelopsis grossedentata extract, astragalus membranacus root extract and other components. According to the invention, an easily oxidized and unstable active matter is fully and firmly protected by adopting a droplet microfluidic coating technology, so that the active matter is free from external stimulation, active ingredients with different effects play multiple roles, and the composition has good anti-inflammatory, soothing and oil-controlling effects and can be applied to the field of cosmetics.

The present invention provides a culture medium and method for high-throughput screening of probiotics from fermented Chinese medicine by microfluidic technology, which relates to the field of microbial technology, wherein Tween-80 is removed from the culture medium. The droplet spectrum baseline formed is flat, and the proportion of positive bacteria screened is significantly increased. Single bacteria from a sample colony are embedded in a droplet containing the above-mentioned culture medium through a microfluidic system. A single run experiment of droplet microfluidic technology can process about 5,000 droplets. The droplets are cultured and screened through a set target OD value range to obtain positive droplets, and the positive droplets are placed in a functional screening culture medium to culture and obtain potential probiotics. It was found through experimental verification that the number and abundance of potential probiotics screened by microfluidic technology are superior to traditional screening methods, greatly reducing the time and labor costs of screening.

The invention belongs to the technical field of microfluidics, and particularly relates to an integrated liquid drop micro-fluidic chip based on 3D printing and a liquid drop preparation method. Comprising a continuous phase channel unit and a dispersed phase channel unit, the continuous phase channel unit comprises a main channel unit, and a plurality of main split channels are communicated in the circumferential direction around an outlet of the main channel unit; the dispersed phase channel unit comprises a branch channel unit, a plurality of branch splitting channels are circumferentially communicated around an outlet of the branch channel unit, a droplet control structure is arranged at the communication part of the main splitting channel and the outlet of the branch splitting channel, and a droplet generation channel is arranged at the communication part of the outlet of the branch splitting channel and the outlet of the main splitting channel; an outlet of each liquid drop generation channel is communicated with a collection opening; the multiple collection openings communicate with the amplification section structure, and an outlet of the amplification section structure communicates with a total collection opening. The three-dimensional space is fully utilized, high-flux and uniform preparation of the liquid drops can be achieved, and the method is suitable for large-scale production of the liquid drops.

The invention belongs to the technical field of molecular biology, and particularly relates to a method for screening algae cells. The method comprises the following steps: mixing a suspension of algae cells with a lipid dye for dyeing to obtain a dyed algae cell suspension; taking the dyed algae cell suspension as a water phase, and generating liquid drops from the water phase and the oil phase through a liquid drop micro-fluidic technology; and separating the liquid drops with the optical signals higher than the reference value, wherein the liquid drops contain the target algae cells. Through a liquid drop sorting method, a reaction system is improved to picoliter grade, finer control and operation are realized, high-flux and high-precision screening in a microenvironment is facilitated, and the problem that the reaction system is limited is solved. In addition, the screening cost of a single strain is also reduced. By adopting a method of firstly dyeing and then generating the liquid drops, omission possibly occurring in the fusion process of the liquid drops and the dye liquid drops is avoided, and fluorescence detection errors caused by different dye concentrations in the algae cell dyeing and liquid drop sorting process are avoided.

The invention provides a digital liquid drop micro-fluidic chip system and a bacteria detection method, and relates to the technical field of microbiological detection, the digital liquid drop micro-fluidic chip system comprises a micro-fluidic liquid drop generation chip, a micro-fluidic liquid drop fusion chip and a micro-fluidic liquid drop reaction chip. The bacterium detection method comprises the step of carrying out bacterium detection on a sample by using the digital droplet micro-fluidic chip system. The digital droplet micro-fluidic chip system and the bacterium detection method have the advantages of high detection flux, high stability, high sensitivity, rapidness, low cost and the like, are suitable for simultaneous detection of various bacteria in unknown samples, and can be used in the fields of environmental microorganism detection, food-borne pathogenic bacterium detection, clinical pathogenic bacterium diagnosis and the like.

The invention discloses a hydrogel microsphere sorting method, an artificial organ preparation method, a system and a medium. The method comprises the following steps: acquiring a hydrogel microsphere image in the micro-fluidic chip; analyzing the hydrogel microsphere image through a neural network to obtain an analysis result; based on the analysis result, hydrogel microspheres wrapping the target cells are sorted out; the neural network comprises a feature extraction layer and a multi-scale joint output head, and the hydrogel microsphere image is processed by the multi-scale joint output head after being convolved by the feature extraction layer to obtain an analysis result. According to the method, the hydrogel microspheres wrapped with the target cells are accurately identified and sorted by using a droplet microfluidic technology, a standardized artificial organ can be prepared based on the sorted hydrogel microspheres, and the functional maturity and reliability of the artificial organ are remarkably improved.

The invention relates to the field of single cell analysis of droplet microfluidics, in particular to a controllable cell lysis method in an emulsion. The invention provides a method for cell lysis in a liquid drop, which comprises the following steps: 1) liquid drop single cell wrapping: wrapping a single cell into the liquid drop through a micro-fluidic chip in the presence of a first oil phase to obtain a first mixture containing the first oil phase and the liquid drop; and 2) cell lysis: replacing the first oil phase in the first mixture with a second oil phase containing a cell lysis solution, and incubating to realize cell lysis. For realizing cell lysis in the liquid drop, the second oil phase containing the cell lysis solution can realize cell lysis of the liquid drop outside the micro-fluidic chip, so that the operation difficulty is low, the cell lysis method is simple and efficient, low-cost, high-throughput and controllable cell lysis can be realized, and the applicability is wide.

The invention discloses a reusable blood coagulation detection device based on a droplet microfluidic technology and a use method, and belongs to the technical field of droplet microfluidics, and the device comprises a microfluidic chip, an injection pump and an optical detection device. The micro-fluidic chip is used for generating and capturing blood droplets and comprises a PDMS layer, a three-dimensional electrode and a glass substrate, the PDMS layer and the glass substrate are bonded, and the three-dimensional electrode is poured in a second flow channel of the PDMS layer. The injection pump is used for controlling constant flow and is connected with an inlet of the PDMS layer. The optical detection device is used for detecting the blood coagulation process in the micro-fluidic chip and comprises a light source, a first lens, a second lens and a photoelectric sensor. The liquid drops in the micro-fluidic chip are controlled by applying an external electric field, the blood liquid drops are captured and released, the blood coagulation process is monitored by adopting an optical method, and a new thought is provided for blood coagulation function detection. The chip is prevented from being blocked when blood is coagulated mainly in a mode of wrapping the blood by an oil phase, and possibility is provided for flushing and reusing the chip.

The invention relates to a high-throughput screening method of alpha-1, 2-fucosyltransferase and a mutant obtained by screening, and belongs to the technical field of enzyme engineering. The invention provides a high-throughput screening method of alpha-1, 2-fucosyltransferase (alpha-1, 2-fucosyltransferase). The method comprises the following steps: constructing an indicator strain of 2 '-fucosyllactose, enabling the content of the 2'-fucosyllactose to be positively correlated with the fluorescence intensity, then expressing an L-fucokinase and alpha-1, 2-fucosyltransferase mutant library in a chassis strain, carrying out water-in-oil droplet embedding on the chassis strain, and carrying out fusion co-culture on the chassis strain and the indicator strain to obtain the 2 '-fucosyllactose. A chassis strain containing the alpha-1, 2-fucosyltransferase forward mutant is obtained through droplet microfluidic sorting according to a fluorescence detection result, the enzyme activity of the mutant FutCV93I is 2.31 times that of an original enzyme, and the enzyme activity is greatly improved. By using the method provided by the invention, the alpha-1, 2-fucosyltransferase can be subjected to high-throughput screening, and industrial production of the 2 '-fucosyllactose and large-scale application and popularization of the 2'-fucosyllactose in the fields of foods, medicines and the like are facilitated.

The utility model provides a digital droplet micro-fluidic chip system and relates to the technical field of microbiological detection. The digital liquid drop micro-fluidic chip system comprises a micro-fluidic liquid drop generation chip, a micro-fluidic liquid drop fusion chip and a micro-fluidic liquid drop reaction chip. The microfluidic liquid drop generation chip comprises n liquid drop generation units, wherein each liquid drop generation unit comprises a first dispersed phase inlet, a second dispersed phase inlet, a dispersed phase mixing channel and a liquid drop generation area; the dispersed phase mixing channel is of a snakelike structure, a broken line structure or a spiral structure; the liquid drop generation area comprises a cross intersection, and the cross intersection is of a closing-up structure which is in a trapezoid shape. The digital droplet micro-fluidic chip system disclosed by the utility model has the advantages of high detection flux, high stability, high sensitivity, rapidness, low cost and the like, is suitable for simultaneously detecting various bacteria in an unknown sample, and can be applied to the fields of environmental microorganism detection, food-borne pathogenic bacterium detection, clinical pathogenic bacterium diagnosis and the like.

The invention provides a room-temperature high-chemical-yield ultrasonic enhanced droplet microfluidic synthesis method of perovskite quantum dots. The method comprises the following steps: manufacturing a droplet microfluidic reaction chip with a two-section series double-T-shaped structure, and coupling the droplet microfluidic reaction chip with an ultrasonic vibrator to form a reaction system; the precursor solution, the ligand solution and the acetonitrile solution are injected into a droplet microfluidic reaction chip in proportion, and an ultrasonic vibrator is started to carry out ultrasonic enhancement on the reaction solution. And centrifugally drying the product to obtain the perovskite quantum dots. Compared with the prior art, the method has the following beneficial effects: 1, mass transfer between two phases is accelerated through ultrasonic treatment, the reaction process is enhanced, and the chemical yield and efficiency of the quantum dots are improved; 2, the size of the quantum dots can be regulated and controlled by adjusting the flow rate ratio and the ultrasonic power; 3, the specific surface area of reactants can be increased by utilizing the constructed reaction system, and the reaction conditions can be accurately controlled; and 4, the reaction system is high in automation compatibility degree and suitable for distributed continuous production.

The present application relates to the technical field of microfluidic chip, and disclose a centrifugal microfluidic chip for generating different size droplets simultaneously, including a liquid distribution channel for uniformly distributing sample to multiple droplet generation units, the design of a quantitative pool ensures the fixed volume of liquid and the consistency of the number of droplet generation, the chip contains a sample inlet and two continuous phase reagent inlets, the sample is precisely distributed to the quantitative pool by centrifugal force and enters the droplet generation units respectively, the first droplet generation unit is used for generating large size droplets with a diameter of 100-200 mu m, and the second droplet generation unit is used for generating small size droplets with a diameter of 10-100 mu m, the microfluidic chip can realize the preparation of ultrahigh throughput droplets, the generated droplets are guided to the collection area by centrifugal force to form a regular single-layer arrangement, the chip has wide application in the fields of biomedical research, material science, chemical synthesis and environmental monitoring, especially in the preparation of fluorescent microspheres, which can improve the experimental efficiency and result consistency.

The invention discloses an electrically-driven integrated liquid drop microfluidic device, relates to the technical field of microfluidics, and is used for solving the technical problems that most of the existing control modes are limited to plane control and cannot adapt to unstructured working environments in a deep cavity and a biochemical analyzer. The electrically-driven integrated liquid drop microfluidic device comprises a supporting plate, a liquid collecting plate, a detection platform, a displacement mechanism and a liquid transferring mechanism, the supporting plate is provided with a first surface and a second surface which are oppositely arranged; the supporting plate is arranged on the fixed platform through the first surface; the liquid collecting plate is arranged on the supporting plate through the second surface; the detection platform is arranged at any end of the liquid collection plate; the displacement mechanism is arranged to move in the horizontal and / or vertical direction relative to the liquid collecting plate; the pipetting mechanism is arranged on the displacement mechanism and is used for moving the liquid drops or the liquid marbles from the liquid collection area to the detection platform through dielectrophoretic force.

The application discloses a kind of based on digital droplet microfluidic multi-sample flux multi-index detection device and application thereof, belong to electromechanical device, microfluidic chip and immunoprotein analysis field.The device includes droplet microfluidic equipment and immunoprotein analysis chip component, droplet microfluidic equipment is used to drive multiple droplets, mainly divided into three major systems: power supply system, computer operating system and chip clamp system, immunoprotein analysis chip component includes biological analysis substrate and microarray chip.The present application drives micro liquid sample by digital droplet microfluidic technology, realizes the multi-index detection of automated immunoprotein analysis chip.The detection device of the present application has a series of advantages such as rapidity, independence, low consumption, automation, function integration and multi-index detection, and is expected to become the most fruitful rapid diagnostic device for improving tumor diagnosis positive rate.

An automatic system which combines a three-branch sorter with three independently-controlled valves and corresponding collection platforms. The subgroups of droplets are alternatively sorted into three channels followed by being pumped out into PCR tubes on the platforms. Experimentally, this system can realize an accurate collection with a large working range for both pure positive samples and mixed samples with negative ones. This technology effectively dispenses a large number of droplets into small subgroups with quantitative number, which builds the basis for digital droplet microfluidics.

The application provides a preparation method of organoids based on a single cell chip, which comprises microfluidic chip preparation, a two-water-phase solution preparation, a single cell suspension preparation, microfluidic manipulation, single cell-loaded microgel generation and organoid construction and the like. The application takes a microfluidic chip integrated with a normally closed pneumatic pump valve as a technical platform, takes a single cell suspension pre-incubated with a crosslinking agent as a chemical reaction core, takes a water-in-water droplet as a forming template, and realizes efficient in-situ loading of single dispersed seed cells in a micro hydrogel carrier through a one-step method, and simply removes empty droplets without cells in a subsequent transfer step. The single cells in the microgel can be cultured, expanded and self-organized to form required organoids. The application improves the consistency of the starting point of the organoids, has the high-throughput characteristics of the droplet microfluidic technology, realizes efficient and highly reproducible in-vitro construction of the organoids, and can play a great role in the fields of basic research and transformation application related to the organoids.

The invention relates to the technical field of polymer microparticle preparation, in particular to a special-shaped polymer microparticle preparation method which comprises the following steps: taking a polymer-containing solution as an inner oil phase and a polyvinyl alcohol aqueous solution as an outer water phase, generating micro-droplets by using a droplet micro-fluidic chip, and standing the micro-droplets under a constant-temperature condition to volatilize a solvent; and by regulating and controlling the components and proportion of the polymer and the concentration and constant temperature of the polyvinyl alcohol aqueous solution, the micro-droplets are induced to generate controllable phase separation or interface self-assembly, and are finally cured into the special-shaped polymer microparticles. According to the invention, controllable preparation of spherical, dumbbell-shaped, raspberry-shaped and other polymorphic microparticles is realized through a droplet microfluidic technology and a solvent volatilization coordinated regulation strategy. The method has the characteristics of universality, high repeatability and simple and convenient process, can adapt to various polymer systems, and meets the diversified requirements of the fields of biomedicine, catalysis, functional materials and the like on microparticles with complex shapes.

Single-cell methods are provided for investigating DNA and RNA interactions with regulatory proteins and evaluating the cytotoxicity of immune cells towards target cells, such as tumor cells. The methods utilize droplet microfluidics and imaging-based technology to facilitate the isolation and analysis of protein-bound nucleic acids from individual immune cells or apoptotic tumor cells. The methods utilize fluorescence-activated droplet sorting and are capable of isolating and characterizing mitochondrial DNA from cytotoxic immune cells.

The invention discloses an intelligent preparation and control method for nano metal powder driven by a droplet microfluidic technology, relates to the technical field of metal powder processing, and aims to accurately control particle distribution, calculate particle size distribution uniformity Dcv and dispersion stability coefficient Dsf and optimize a shear flow field through a droplet interface dynamic model in combination with a dynamic light scattering and adaptive optimization algorithm. The particle size uniformity is ensured, the powder stacking density and the conductive stability are improved, and the problem that particle size distribution is difficult to accurately control in an existing method is solved; a high-sensitivity sensor is adopted to monitor environmental interference, Fourier transform analysis and Gaussian process regression reinforcement learning are combined to optimize temperature and flow velocity, by-products and pollutants are reduced, powder purity is ensured, long-term stability of electronic devices is improved, and resistance drift or local overheating is avoided. And aiming at the agglomeration problem, an agglomeration trend value Pag is calculated and graded evaluation is carried out, so that particle agglomeration is effectively inhibited, and the stability and reliability of the conductive material are improved.

The invention discloses a method and a device for preparing liquid drops by using a liquid drop generating device in the technical field of liquid drop microfluidics, and the device comprises a liquid drop receiving tank for containing continuous phase liquid, a liquid drop generating mechanism for producing the liquid drops and a micro-vibration mechanism for providing a vibration source, wherein the vibration output direction of the piezoelectric actuator is perpendicular to or approximately perpendicular to the length direction of the pointed-end pipe, so that the continuous-phase liquid near the nozzle can conveniently form an acoustic vortex effect to promote the formation of liquid drops. The method for preparing the liquid drops comprises the following steps: S1, respectively preparing a dispersed phase liquid and a continuous phase liquid; s2, extending a nozzle to a depth h below the liquid level of the continuous-phase liquid; s3, working parameters of the piezoelectric actuator are set, the working parameters comprise the voltage U and the sine wave frequency f1, the voltage U ranges from 20 V to 120 V, and the sine wave frequency f1 ranges from 2 kHz to 20 kHz. By triggering the local acoustic vortex effect in the continuous phase, the formation of the liquid drops is promoted, and the purposes of reducing the particle size variation of the liquid drops and improving the production efficiency of the liquid drops are achieved.

The invention relates to the technical field of microfluidics, in particular to a droplet micro-fluidic chip. Comprising a main pipeline, a first pipeline and a second pipeline, the first pipeline and the second pipeline are respectively communicated with the main pipeline, and the first pipeline and the second pipeline are respectively positioned on two opposite sides of the main pipeline; the same liquid flows in the first pipeline and the second pipeline. The hydrogel microspheres loaded with the hiPSCs not only are beneficial to transfer and control of the hydrogel microspheres loaded with the hiPSCs, but also can load different types of cells in the shell and core space of the microspheres, thereby being beneficial to research on the interaction relationship between cells or organoid derived from the cells.

The invention relates to the technical field of micro-nano sensing and detection, in particular to a temperature sensing method based on a micro-nano robot. The invention discloses a temperature detection method based on a micro-nano robot. The temperature detection method comprises the following steps: step 1, preparing a temperature sensitive material by using copper tetraamminesulfate particles and resin; 2, preparing a micro-nano robot with a temperature detection function by adopting a droplet microfluidic technology and a physical vapor deposition technology based on the temperature sensitive material in the step 1; 3, extracting color features by using the micro-nano robot with the temperature detection function obtained in the step 2, and building a temperature detection regression prediction model based on the color features; and 4, on the basis of the temperature regression prediction model obtained in the step 3, the micro-nano robot with the temperature detection function is used for carrying out temperature detection on the micro gap, and the average value of the detection results of the multiple micro-nano robots is taken as the final detection temperature. The problem that an existing temperature detection method in the industrial field is limited is solved.

Provided are a single-cell chromatin accessibility and transcriptome combined sequencing method and application thereof. The method comprises: treating a cell nucleus using a transposase to obtain gDNA of a chromatin open region connected with a first specific tag sequence; treating the cell nucleus using a transcriptome capture sequence to obtain cDNA connected with a second specific tag sequence; generating a droplet based on a droplet microfluidic to seal the cell nucleus and a first microbead in the droplet, wherein the gDNA and the cDNA are captured by the first microbead, and wherein more than one cell nucleus is sealed in the droplet; and sequencing the gDNA and the cDNA to obtain combined information of single-cell chromatin accessibility and transcriptome according to the first specific tag sequence and the second specific tag.