Integrated liquid drop microfluidic chip

Abstract

An integrated liquid drop microfluidic chip comprises one or more liquid drop generation structures, every liquid drop generation structure is connected with a corresponding collection cavity structure through a corresponding connection channel, a water phase reagent and an oil phase reagent respectively go through the one or more liquid drop generation structures to generate liquid drops with uniform dimension, an emulsion including the liquid drops goes through the connection channel and directly or indirectly enters the collection cavity structure, and is adaptively arranged to form a regular liquid drop array in the collection cavity structure, the liquid drop array undergoes a biochemical reaction under constant temperature control or temperature circulation control in an air heating refrigeration or Peltier semiconductor heating refrigeration mode, and real time monitoring or timed optical detection is also carried out. The integrated liquid drop microfluidic chip reduces the sample transfer process, can be conveniently integrated with a thermometric module and an optical detection module, greatly improves the reaction and detection speed, and also simplifies the complex degree of a whole apparatus system.

Description

technical field [0001] The invention belongs to the technical field of biochemical microfluidics, and in particular relates to an integrated droplet microfluidics chip. Background technique [0002] At present, biochemical analysis and detection based on droplet microfluidic technology has been fully applied and developed, such as QX200 TM and Raindrop TM Digital PCR system, these two systems have greatly improved the detection sensitivity, accuracy and measurable concentration dynamic range, but they still cannot fully meet the needs of biochemical detection in practical applications. On the one hand, these two systems have high requirements for peripheral control components, which leads to increased system complexity and difficulty in reducing system costs; It is often necessary to transfer between different instruments, which makes the degree of automation and integration of the system not high. In addition, the amplification reaction and flow detection of the system t...

AI Technical Summary

Problems solved by technology

Firstly, the chip will inevitably cause the loss of droplets, which will affect the detection results; secondly, air bubbles are easy to be generated in the cavity of the chip, and these bubbles will continuously expand and contract during the PCR amplification process, which is extremely dangerous. may lead to the failure of the experiment

Finally, the arrangement structure of the droplets in the chip cavity is heavily dependent on the ratio of water to oil in the early stage of droplet formation, which will inevitably bring operational difficulties and limit the later application

[0004] Document CN103343092 mentions a method for making a digital PCR chip based on mineral oil-saturated PDMS material, but because PDMS material has a super strong absorption capacity for oily molecules, and the strength of PDMS material is poor, it is easy to deform, so It is mostly used for laboratory research and cannot meet the application requirements of industrialization

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