96 results about "Laboratory research" patented technology

The invention relates to the technical field of biological catalysis, and discloses an M88F mutant enzyme for preparing rebaudioside I. The enzyme is obtained by the following mutations generated by UGT76G1: M88F: methionine of the 88th amino acid sequence of UGT76G1 is mutated into phenylalanine; and the substrate rebaudioside A (RA) can be efficiently and directionally converted into rebaudioside I (RI) with higher value. In an optimized reaction system, the conversion rate stably reaches 40%, and the catalytic efficiency is improved by more than 5-8 times compared with the common reference enzyme. The enzyme preparation has the characteristics of high catalytic activity, mild reaction conditions, simplicity and convenience in operation and the like, shows good stability and reproducibility in laboratory research and large-scale production, and has a wide industrial application prospect.

The invention discloses a combined treatment medicine for acute myelogenous leukemia. The combined treatment medicine is prepared from vinaclaria, azacitidine and aclarithromycin. The medicine is applied to preparation of an acute myelogenous leukemia cell proliferation inhibitor; the medicine is applied to preparation of an acute myelogenous leukemia cell apoptosis inducer. The three medicines are combined to achieve a synergistic effect: the three medicines have a synergistic anti-tumor effect instead of a simple superposition effect, and a new combined medication mechanism is a result obtained through laboratory research and an initial effect of clinical application. The subsequent consolidation treatment scheme may also be used for part of initially treated patients with acute myelogenous leukemia, which are not suitable for enhanced chemotherapy or intense intense treatment by using targeted drugs, and relapse / refractory patients with acute myelogenous leukemia, and a better treatment scheme can also bring new hope and dawn to more patients.

The invention relates to the technical field of tobacco manufacturing, in particular to a method for characterizing the quantitative relaxed feeling of an aerosol generating product, the characterization method realizes the conversion of the cigarette smoking relaxed feeling from subjective qualitative feeling to objective quantitative feeling, through accurate sensor monitoring and scientific algorithm calculation, the individual difference of evaluation is greatly reduced, and the evaluation accuracy is improved. Different laboratories and researchers can obtain quantitative results with high repeatability and high credibility according to the method; a powerful tool is provided for a cigarette research and development link, research and development personnel can adjust elements such as a cigarette formula and a filter tip structure in a targeted manner according to quantitative data, and the smoking easiness is accurately optimized; the invention further provides a characterization device for the quantitative suction relaxed feeling of the aerosol generating product, the characterization device comprises a flow sensor A for obtaining airflow flow Q (t), a pressure difference sensor B for obtaining dynamic suction resistance R (t), a time sequence control unit, a suction machine, a power module and a calculation module, the characterization device is used for achieving the steps of the characterization method, and the related computer product is further provided.

The invention relates to a specific antibody aiming at CD228 protein and an antigen binding fragment thereof, which are used for detecting and treating various tumors, such as melanoma, mesothelioma, colorectal cancer and the like. The antibody binding fragment is helpful for diagnosis and treatment of cancers through specific binding with CD228. The invention further comprises antibody drug conjugates (ADCs) which bind a drug to an antibody through a linker, so as to improve the therapeutic effect and reduce the influence on normal cells. Meanwhile, nucleic acids encoding the antibodies or antigen-binding fragments thereof are provided to produce recombinant antibodies or fragments. Besides, the invention also provides a kit which is used for laboratory research, clinical diagnosis and treatment effect monitoring, is beneficial to determining whether tissues or cells express CD228 or not, and provides a new strategy for personalized and precise treatment of cancers.

The invention relates to the field of high-voltage electrical engineering, in particular to a GIS insulation structure physical model construction method, system and device and a medium, and the method comprises the steps: obtaining first electric field distribution data of a GIS insulation structure under a preset voltage; based on the first electric field distribution data, constructing an equivalent electric field model of the GIS insulation structure; calculating second electric field distribution data of the equivalent electric field model by using finite element electric field simulation; the equivalent electric field model is adjusted, so that the difference between the first electric field distribution data and the second electric field distribution data reaches a preset difference condition, and an optimal equivalent electric field model is obtained; and constructing a physical model of the GIS insulation structure based on the optimal equivalent electric field model. According to the method, the engineering representativeness and accuracy of data obtained by performing experimental researches such as partial discharge by using the physical model are greatly improved, and a solid foundation is laid for reliable conversion of laboratory research results to actual engineering application.

The application provides a method for detecting impurities in leboregen starting material LBS5, and belongs to the field of pharmaceutical analysis chemistry. S ,5 R )‑1‑(3‑fluorophenyl)‑3‑oxabicyclo[3.1.0]hexan‑2‑one. The method adopts high performance liquid chromatography, can simultaneously and accurately quantify one degradation impurity and four process impurities in LBS5, and has strong specificity, high sensitivity, good accuracy, good reproducibility and stable and reliable method. The method can realize the control of the impurities, ensures the safety of LBS5 as a pharmaceutical intermediate, further ensures the safety of a preparation of LBS5, reduces the risk of drug use, simultaneously reduces the difficulty of subsequent synthesis steps, realizes rapid, economic and environmental protection production. The method is suitable for laboratory research and development, and is also suitable for quality control in commercial production.

The invention discloses a high-density homogeneous green anode carbon block dynamic formula and a forming parameter optimization method, belongs to the technical field of electrolytic aluminum carbon, and aims to solve the problems that control indexes in a green anode formula and a manufacturing process are too simple, and the quality of a prebaked anode cannot be well controlled and improved. The method comprises the following steps: analyzing the quality of calcined coke for raw anode carbon block burdening; the method is suitable for dynamic formula technology laboratory research of different raw material conditions; quickly analyzing the quality condition of the calcined coke and establishing a feedback system; analyzing the viscosity of the asphalt; and performing a kneading forming process parameter optimization test. According to the method, multi-factor and all-around technology and process research are integrated, the key technology for producing the high-density homogeneous green anode from related raw materials is broken through, high-density homogenization of the green anode volume density index is achieved, the quality of the green anode is remarkably improved, the problem that the volume density of the green anode is not uniform due to quality fluctuation of calcined coke is solved, and the method is pioneered in the industry and has wide application prospects. And the practical value is extremely high.

The application discloses an evaluation method and system for coke quality entering a furnace by using an industrial CT. The evaluation method comprises the following steps: selecting multiple pieces of coke entering the furnace as samples to obtain coke samples A and B to be measured; scanning the original coke samples A and B to be measured by using the industrial CT to obtain original crack and pore characterization values of the coke samples A and B to be measured; treating the coke samples A and B to be measured after processing by using heat treatment and alkali metal corrosion, and then scanning the coke samples A and B to be measured after processing by using the industrial CT to obtain internal crack and pore characterization values of the coke samples A and B to be measured after heat treatment and alkali metal corrosion treatment; processing the internal crack and pore characterization values of the same positions collected, and evaluating the coke quality entering the furnace according to the processing results. The method solves the limitation of observing the coke crack structure in the steel enterprises, is simple to operate, is accurate in result, is suitable for popularization and application in the steel enterprises and laboratory research, and has a good application prospect.

The invention discloses a preparation method of resistance-type strain sensing hydrogel, and belongs to the technical field of gel materials.The preparation method comprises the steps that surface modification is conducted on carboxylated carbon nanotubes through levodopa, so that the CNTs form stable water-phase dispersion liquid; meanwhile, the strong hydrogen bond interaction between the catechol group and the functional group on the surface of the MXene is utilized, so that the DCNT and the MXene are stably combined, the oxidation resistance of the MXene is enhanced, and the mechanical property and the conductivity of the MXene hydrogel are improved; the defects that MXene is easy to oxidize and CNT is poor in dispersity in water are effectively overcome; the preparation method has the advantages of simplicity and convenience in operation and cost controllability, and effectively promotes the conversion process of the flexible electronic device from laboratory research to practical application.

This invention discloses a bioreactor for plant cell culture, comprising a culture container body, a liquid replenishment system, a sampling unit, a stirring and aeration system, and an exhaust gas treatment device. The culture container is made of high borosilicate glass, with the top sealed by a GL45-PP cap and a four-hole stainless steel plate. Externally, it is connected to the liquid material inlet, sampling unit, aeration system, and exhaust gas treatment device via silicone tubing. Gas is bubbled into the culture system through filters in the sampling unit and aeration system, utilizing bubbles and airflow to achieve stirring and realize suspension culture of plant cells. This invention, through the use of a high borosilicate glass culture container, pressure difference-based liquid replenishment, and a convenient sampling unit, achieves continuous plant cell culture, improves the safety, stability, and efficiency of the culture system, enhances cell culture results and the yield of plant secondary metabolites, and meets the needs of laboratory research and industrial production.

The invention provides a bromo-oxime ether content detection method which is characterized by comprising the following steps: adding a bromo-oxime ether reaction solution into an anhydrous aprotic polar solvent, adding a sodium alkoxide solution for reaction, and purifying to obtain a standard sample of a bromo-oxime ether sodium alkoxide derivative; adding the bromo-oxime ether reaction solution into an anhydrous aprotic polar solvent, and adding a sodium alkoxide solution for reaction to obtain a to-be-detected sample of the bromo-oxime ether sodium alkoxide derivative; and analyzing the standard sample and the sample to be detected by adopting a high performance liquid chromatography external standard method to obtain the content of bromo-oxime ether in the bromo-oxime ether reaction liquid. The method is high in detection accuracy, good in repeatability and suitable for being applied to the scenes of laboratory research and development, factory production and the like.

The present invention provides a method for detecting impurities in lomepirlone starting material LMS1, belonging to the field of pharmaceutical analytical chemistry. The present invention provides a method for detecting multiple impurities in lomepirlone starting material LMS1. This method can accurately quantify three isomers and three process impurities in lomepirlone starting material LMS1 simultaneously, and has strong specificity, high sensitivity, good accuracy, good reproducibility and stable and reliable method. Using this method, the control of these impurities can be achieved, ensuring the safety of lomepirlone starting material LMS1 as a pharmaceutical intermediate, thereby further ensuring the safety of its preparations and reducing the risk of medication. This method is applicable to laboratory research and development, and is also applicable to quality control in commercial production.

The application discloses a traditional Chinese medicine composition for treating lung cancer and a preparation method thereof. The traditional Chinese medicine composition comprises the following raw medicinal materials in parts by weight: Fritillaria thunbergii Miq. 5-8 parts, Caesalpinia sappan 5-8 parts, Polygonatum odoratum 5-8 parts, Iridaceae 5-8 parts, Platycodon grandiflorum 5-8 parts, Inula japonica 5-8 parts, Sasa veitchii 22-25 parts, Humulus scandens 9-12 parts, Bidens pilosa 22-25 parts and Glycyrrhiza uralensis 2-5 parts. The traditional Chinese medicine composition is developed on the basis of the phlegm and blood stasis blocking lung pathogenesis of lung cancer in traditional Chinese medicine diagnosis and treatment, combines the phlegm and blood stasis treatment method of traditional Chinese medicine and laboratory research, and has the effects of resolving phlegm and removing blood stasis and inhibiting tumor growth, and has a good effect on the treatment of lung cancer. Experiments prove that the traditional Chinese medicine composition significantly inhibits lung cancer cell proliferation in vitro experiments and improves the lung cancer pathological process of lung cancer mice in vivo experiments, and is a great innovation of a lung cancer treatment drug.

The invention discloses a method for promoting sterile germination and seedling formation of paphiopedilum brevifilifolium seeds. The method comprises the following steps: selecting pods of paphiopedilum leucopeniculatum as explants, disinfecting the pods, and cutting the pods to obtain sterile seeds of the paphiopedilum leucopeniculatum; mixing the disinfected and sterilized paphiopedilum brevifilifolium seeds with sterile water to prepare a seed suspension, and sowing the seed suspension on a culture medium for dark culture. The application of the invention provides a practical and efficient technical path for artificial propagation and industrial seedling culture of orchid plants. The method is easy and convenient to operate, good in repeatability, suitable for laboratory research and large-scale production and wide in application prospect. By regulating and controlling the synergistic effect of the illumination condition and the carbon source type, the physiological metabolism of the dormant seeds of the paphiopedilum baphicacanthus is effectively activated, the technical bottleneck that orchid plants are difficult to germinate is broken through, orchid resource protection and development of a breeding technology are promoted, and theoretical support and practical basis are provided for sustainable utilization of rare ornamental plants in China.

The invention belongs to the technical field of functional polymer materials and photocatalysis, and particularly relates to a preparation method and application of an antibacterial coating based on polyvinyl chloride plastics. Comprising the following steps: S1, pretreatment; s2, carrying out a reaction; s3, post-processing is carried out; s4, compounding with polyelectrolyte; and S5, preparing the coating. Through a specific catalytic cracking process, the waste PVC is converted into a functional photocatalytic antibacterial coating material, so that resource utilization and functional utilization of the waste chlorine-containing plastic are realized; a catalytic cracking process under a solvent-free condition is adopted, so that the use of an organic solvent is avoided; the coating material has relatively good photochemical stability, and can realize a relatively lasting antibacterial effect; the method has relatively low requirements on equipment, reaction conditions are easy to control, and the method is not only suitable for laboratory research, but also convenient for engineering and industrial amplification. And by combining the processing performance of the obtained coating, the technology has popularization and application potential.

The invention provides a fishing net strength testing device, and relates to the technical field of fishing net detection, the fishing net strength testing device comprises a rod, the rod is provided with a plurality of chutes surrounding the axis array of the rod, and the chutes are provided with end parts and strip-shaped openings; the device further comprises conical blocks corresponding to the sliding grooves, the conical blocks are inserted into the sliding grooves and extend outwards from the strip-shaped openings, and slopes are arranged on the outwards-extending parts and used for making contact with line structures forming meshes. The invention provides two test modes, the first mode is suitable for dynamic performance analysis in a research and development stage, response characteristics of a fishing net material under a complex working condition are captured through a flexible adjusting system, the second mode is specially designed for production quality inspection, and the test efficiency is improved. Standardized batch detection can be achieved through the rigid structure and the modular assembly, the full-chain testing requirement from laboratory research to industrial production can be met through complementation of the two technical routes, and a complete solution is provided for mechanical property evaluation of fishing net materials.

The application discloses a safe, efficient and low-cost cleaning solution for suspended cells and application thereof, which comprises multiple kinds of glucose, amino acid supplements, vitamin supplements, anti-shear protective agents and sodium bicarbonate, is designed for the harvesting and washing process of suspended cells, can effectively remove residual culture medium, cell fragments, metabolic waste and process-related impurities while effectively maintaining cell viability, viable cell density and normal morphology, so as to realize the harvesting target of high-purity, high-activity and low-risk material of suspended cells, and is especially suitable for the production process of high-demand cell products such as cell culture meat. The cleaning solution system can be adapted to the continuous harvesting process of a large-scale bioreactor, can be integrated with automatic harvesting equipment, realizes stable, efficient and repeatable cell collection and washing operation, and meets the full-scene requirements from laboratory research and development to industrial production.

The microscale particle flow and reaction measuring probe comprises a sleeve, the sleeve comprises an inner tube and an outer tube, a light source receiving opening is formed in the outer tube, and a light source is arranged at the position corresponding to the light source receiving opening; an objective lens, a light guide column and an eyepiece are fixedly mounted in the inner tube; an optical fiber bundle is fixed between the inner tube and the outer tube; the device can be connected with imaging equipment with different interfaces, the magnification is changed by adjusting the object distance and the image distance, manual focusing is carried out in combination with a connector, a high-quality dynamic image of micro-scale dynamic particles is obtained, and synchronous measurement of multiple parameters such as concentration, speed, rotation and collision of particles in a multiphase flow system is further achieved through an AI particle tracking algorithm. The structure is simple, the detection speed is high, and the rapid detection requirements of laboratory research and industrial production can be met.

The application discloses a method for regulating bubble size in a high-temperature molten salt bubbling tower, comprising the following steps: according to the diameter range of the target bubble required by the bubbling tower, a single-hole bubbling experimental device is built by selecting a bubble distributor with a corresponding characteristic aperture; according to the component of the molten salt required by the bubbling tower, a database of the relationship between the molar ratio of each chemical component of the molten salt and the physical properties is established; in the single-hole bubbling experimental device, a regulation mode is selected according to the size of the characteristic aperture of the bubble distributor, the adjustable range of the bubble diameter is adjusted to the diameter range of the target bubble required by the bubbling tower; and the obtained regulation mode is applied to the bubbling tower; the application can reveal a comprehensive solution of the synergistic mechanism among multiple parameters, so as to realize accurate and predictable control of the bubble size from microns to millimeters, and solve the technical problems from laboratory research to industrial device amplification.

The present invention discloses a hybridoma cell line that stably secretes monoclonal antibodies against mink Aleutian disease virus and its application, belonging to the field of biotechnology. The monoclonal antibody of the present invention is secreted by the hybridoma cell line with the deposit number CGMCC No. 23875. The hybridoma cell line has the ability to stably secrete IgG1 antibodies, and the monoclonal antibodies produced are high in titer and single in subclass, and can specifically recognize ADV VP2 protein. 492 FPHEV 496 The antigen epitope is 100% homologous to 24 ADV strains published domestically and internationally. It reacts specifically with ADV strains or proteins and can be used as a diagnostic reagent for mink Aleutian disease. It has practical application value and provides a material basis and technical support for clinical differential diagnosis and laboratory research of mink Aleutian disease.

The present application relates to a kind of molybdenum disulfide nanosheet and its large-scale preparation method, and the preparation method comprises the following steps: sulfonic acid group precursor is dissolved in water with trinitro-pyrene ultrasonic, hydrothermal reaction is carried out, the initial product is filtered, dialysis and drying, obtain graphene quantum dot powder;Graphene quantum dot powder is mixed with molybdenum disulfide powder and carries out ball milling reaction, centrifugal separation and washing, obtain molybdenum disulfide nanosheet composite sheet.In the ball milling process, graphene quantum dot has abundant surface functional groups, increases the electron density of sulfur atom and molybdenum atom and then controls molybdenum disulfide structure to form nanosheet.Compared with prior art, the preparation method of the present application is simple, low in cost, mild in reaction, small in toxicity and simple in post-processing, can prepare macro (gram level), good quality, colloid state stable molybdenum disulfide nanosheet, the method for preparing nanoscale molybdenum disulfide nanosheet is suitable for both laboratory research and industrial mass production.

The invention discloses a kit for detecting intestinal core bacteria by a TaqMan real-time fluorescent quantitative PCR (Polymerase Chain Reaction) method, and belongs to the technical field of kits. The kit disclosed by the invention comprises standard positive templates of nine common intestinal core bacteria and nine groups of specific primers and probes which are required in a PCR amplification process and correspond to the standard positive templates. The kit disclosed by the invention is simple, convenient and rapid to operate; the specificity is good and the sensitivity is high; complicated raw information data analysis does not need to be carried out; the intestinal core bacteria can be detected and quantified without high-throughput sequencing, and three bacteria can be detected in one reaction hole, so that the cost is greatly reduced. The kit provided by the invention can be used for rapidly and quantitatively detecting nine intestinal core bacteria including bifidobacterium, lactobacillus, prevotella, bacteroides and the like, can partially replace a high-throughput detection method which is higher in cost and more complicated to operate according to research and diagnosis purposes, and is suitable for being widely popularized in clinical detection and laboratory research.

The application discloses a loudspeaker acoustic load test system, and relates to the technical field of loudspeaker acoustic load test systems. a : wherein, A s represents a radiation area, R e represents a voice coil DC resistance, L e represents a voice coil inductance, Bl represents a magnetic circuit force factor, R ms represents a mechanical damping, M ms represents a mechanical vibration mass, C ms represents a mechanical compliance. The application is based on a classical electroacoustic system, utilizes a sound impedance calculation relationship of a loudspeaker small signal model, and proposes a sound impedance test system based on loudspeaker T / S parameters. Compared with a traditional sound wave guide method for measuring sound impedance, the application has the advantages of high signal-to-noise ratio, low cost, strong adaptability, high efficiency and convenience, and can be used for parameter measurement and quality control in laboratory research and development and production line batch manufacturing.

The invention discloses a method for detecting the solid solution content of rare earth elements in a steel material, which comprises the following steps: firstly, electrolyzing a steel sample which is ground and polished until the surface has no macroscopic defects in a KCl or LiCl + tartaric acid (C4H6O6) aqueous solution, and filtering the electrolyzed suspension to realize the separation of solid-solution rare earth atoms from the rare earth elements in a second phase; then, concentrating the filtered solution, and cooling to a constant volume; and finally, measuring the concentration of the rare earth element in the solution by using an inductively coupled plasma mass spectrometer (ICP-MS), and calculating by using a formula to obtain the solid solubility of the rare earth element in the steel and iron material. The method can safely, reliably and cheaply measure the solid solubility of rare earth elements in steel and iron materials in laboratory research and production practice, and has the characteristics of high sensitivity, safe experimental process, efficient pretreatment process, cheap and easily available medicines and the like.

The invention discloses a construction method of a tumor organoid microenvironment chip model, the model and application of the model. The construction method comprises the following steps: acquiring a tumor organ and preparing cell precipitate; carrying out enzymolysis and digestion treatment on the cell precipitate, and then adding an extracellular matrix to prepare a tumor organ suspension; the method comprises the following steps: adding a tumor organoid suspension into a micro-fluidic culture carrier, culturing to prepare suspensions of fibroblasts and vascular endothelial cells, respectively adding the two cell suspensions into corresponding hole sites of the micro-fluidic culture carrier, and finally culturing to obtain the tumor organoid microenvironment chip model. The model prepared by the method can more truly simulate the complex tumor microenvironment in vivo, is suitable for drug screening and drug sensitivity detection, is beneficial to improving the accuracy and reliability of research, and accelerates the transformation from laboratory research to clinical application.

The utility model discloses a kind of metal melt pouring casting devices, belong to metal smelting and casting technical field, comprising: support, support is used to support on ground;Tilting frame, tilting frame both sides are rotatably connected with support by pivot, handle is fixedly connected on tilting frame, for placing crucible in tilting frame;Clamping device, clamping device is set on tilting frame, clamping device is detachably connected with crucible.The utility model can realize single independent casting operation when small and medium-sized crucible casting larger mould, can improve the convenience of operation, safety and work efficiency, reduce the dependence on manpower and reduce production risk;The principle and manufacturing process of the device are simple, suitable for laboratory research and development experiment and factory production use.

The invention discloses a power generation boiler unit shutdown active organic amine anticorrosion protective agent, which is prepared by adding 3-10 mg / L of cyclohexylamine, 1-5 mg / L of octanoic acid dicyclohexylamine, 10-30 mg / L of tetramethylmorpholine, 10-20 mg / L of diethylhydroxylamine and 10-20 mg / L of diethylaminoethanol into a solvent, and the solvent is deionized water or demineralized water. The use pH environment of the protective agent is 9.5-10.0, the temperature is 250-450 DEG C, the protective agent is good in water solubility, non-toxic, full-volatile and free of obvious side effects on a thermodynamic system, dissolved solids of the system cannot be increased, and when the protective agent is used, the pH of boiler water is stabilized to 9.5-10.0 by using ammonia water. A laboratory research test and an industrial test prove that the protective agent has good protection performance on metal of a water vapor system of a boiler unit, is convenient to use, has no influence on equipment in the boiler unit system, has an excellent atmospheric corrosion resistance effect, and can be widely applied to shutdown and standby protection of the power generation boiler unit.

The invention relates to the technical field of power plant chemistry, and discloses a urea hydrolyzer material type selection method and screening device.The type selection method comprises the following steps that a type selection pool of urea hydrolyzer materials is determined; determining screening conditions according to the test requirements of the urea hydrolyzer material; continuously recording the macroscopic morphology change of the test piece of the screening test; on the basis of the macroscopic morphology change, a metallographic microscope is selected to observe the microscopic morphology change of the test piece; and combining the macroscopic morphology change with the morphology change found by adopting a metallographic microscope, and sorting the corrosion performance of the materials in the model selection pool. The invention aims to provide a laboratory research method and a screening device for urea hydrolyzer key component material selection. By adopting the research method and the screening device, the corrosion resistance of different materials can be evaluated, so that the purpose of optimal selection of the materials of the key parts of the urea hydrolyzer is achieved. Compared with industrial tests, the method has obvious advantages of low cost and short period.

The invention discloses a KRAS targeted heterocyclic compound synthesis path analysis method based on targeted driving, and belongs to the technical field of KRAS targeted heterocyclic compound synthesis. The method comprises the following steps: firstly, establishing a target point-path adaptation relationship between a synthesis system and a synthesis path and generating a path selection behavior set, then constructing a dynamic synthesis label set to record reaction key parameters, then generating a synthesis behavior data matrix and converting the synthesis behavior data matrix into an error feature matrix, and finally, evaluating the binding synthesis system through the error overlapping degree, and generating a consensus path set. According to the method, the problems that a traditional method depends on experience, dynamic tracking is lacked, errors are uncontrollable, and consensus paths are difficult to screen are solved, the objectivity and accuracy of path selection are improved, the synthesis experiment period is shortened, the research and development cost is reduced, and the method is suitable for laboratory research and development and industrial synthesis scenes of KRAS targeted heterocyclic compounds.

This invention discloses a method and application for detecting F17 Escherichia coli. Using the fimbrial virulence gene ctg_04543 or ctg_04549 of F17 E. coli as a target, a fluorescent quantitative probe is designed based on the nucleotide sequence. Using F17 E. coli genomic DNA as a template, the F17 fimbrial gene ctg_04543 or ctg_04549 is detected by fluorescent quantitative PCR. Based on the fitted standard curve, F17 E. coli in the sample can be quantitatively detected. The F17 E. coli detection method provided by this invention is simple, rapid, quantitative, highly sensitive, and highly specific. It can achieve quantitative detection of F17 E. coli within 2-3 hours, meeting the detection needs of various situations such as laboratory research and animal pathogen diagnosis, and has broad application prospects and extremely high market implementation potential.