240 results about "Spider silk" patented technology

Spider silk proteinencodingnucleic acids, polypeptides and antibodies immunologically specificthereforeare disclosed. Methods of use therefore are also provided.

An artificial polypeptide fiber of the present invention is an artificial fiber containing a polypeptide as a main component, and has a stress of 350 MPa or more and a toughness of 138 MJ/m³ or more. A method for producing an artificial polypeptide fiber of the present invention is a method for producing the artificial polypeptide fiber obtained by spinning a spinning solution (6) containing a polypeptide derived from natural spider silk proteins and performing drawing of at least two stages. The drawing of at least two stages includes a first-stage drawing (3) in wet heat and a second-stage drawing (4) in dry heat. Thereby, the present invention provides high-toughness artificial polypeptide fibers having favorable stress and rupture elongation, and a method for producing the same.

The invention discloses a method for preparing spider silk protein/polylactic acid composite nano-fiber yarn. The method comprises the following steps of preparing a spinning solution, preparing a bath solution and then adopting an improved electrostatic spinning method to guide, heat, wind and shape bunched fibers, so as to obtain the spider silk protein/polylactic acid composite nano-fiber yarn. The composite nano-fiber yarn of the invention can be continuously spun for more than 10 hours and have no broken ends, and a certain amount of spider silk protein effectively reduces the diameter of the fibers and improves the strength of the yarn. In addition, the fracture strength and flexibility of the composite nano-fiber yarn can be further improved through secondary stretching and combined twisting, so that the composite nano-fiber yarn can be applied in biomedical materials and other fields better.

Dental floss that includes at least one filament made of spider silk.

The invention discloses a full-automatic two-sided silk-screen printing integrated machine which comprises a control system, a feed transmission device, a main machine table and a material receiving transmission device. The feed transmission device is connected with the main machine table, the main machine table is connected with the material receiving transmission device, rails are arranged on two sides of the main machine table, a transmission table frame is arranged on the main machine table and can move back and forth on the rails, a material carrying table is arranged on the transmission table frame and connected with a material carrying lifting motor, and the material carrying lifting motor drives the material carrying table to go up and down. The main machine table is divided into five working zones in sequence according to silk-screen printing working process, material sensors corresponding to the working zones are arranged on the rails, the material sensors are all connected with the control system, and the feed transmission device, a feed zone, a first printing zone, a turning zone, a second printing zone, a material receiving zone and the material receiving transmission device are all controlled by the control system. The full-automatic two-sided silk-screen printing integrated machine can achieve two-sided printing of printed circuit boards, reduces workload of workers greatly, and improves production efficiency substantially.

The invention relates to a preparation method of a recombinant spider silk protein/silver-bearing nano wound membrane. The preparation method comprises the steps of dissolving PVA (Polyvinyl Acetate) in H2O to prepare a PVA aqueous solution, conducting electrostatic spinning to prepare PVA nanofiber membrane, uniformly spraying a silver nitrate weak solution onto the PVA nanofiber membrane, drying, dissolving pNSR 16 in formic acid to prepare a formic acid solution of RGD (Arginine-Glycine-Aspartate)-spider silk protein, electrically spinning the formic acid solution of the RGD-spider silk protein into the PVA nanofiber membrane by an electrostatic spinning technology to form a pNSR 16 composite silver-bearing PVA nanofiber membrane, dyring, and obtaining the silver-bearing nano biological wound membrane. The membrane adopts natural protein materials and has bioactivity; as silver nitrate antibacterial components are introduced, the membrane has a significant antibacterial property and can kill most gram-negative bacteria and gram-positive bacteria; with the adoption of the electrostatic spinning technology, the preparation process is simple; and compared with medical gauze, the number of days required by complete healing of animal skin is reduced by approximately one third.

A method of producing polymers of an isolated spider silk protein involves providing a solution of said spider silk protein in a liquid medium at pH 6.4 or higher and/or an ion composition that prevents polymerisation of the spider silk protein. The properties of the liquid medium are adjusted to a pH of 6.3 or lower and an ion composition that allows polymerisation of the spider silk protein. The spider silk protein is allowed to form polymers in the liquid medium, and the resulting spider silk protein polymers are isolated from the liquid medium. The resulting polymers are useful as fibers, films, foams, nets or meshes.

A method for the treatment of spider silk filament for use as a thread or composition in the manufacture of cosmetic, medical, textile, and industrial applications, wherein the spider silk filament, derived from genetically modified organisms, is treated with at least one component selected from the group consisting of vitamins, hormones, antioxidants, chelating agents, antibiotics, preserving agents, fragrances, dyes, pigments, magnetic nanoparticles, nanocrystals, cell adhesion enhancers, thermal insulators, shrinkage agents and cosmetic, medical or dermatological active substances. Textile fabrics obtained by this method are stronger, bio-compatible, bio-degradable and have a higher thermal conductivity. Treated spider silk filament can also be applied in an oil-in-water or water-in-oil protective cream that is hypoallergenic and ensures a firmer skin.

Load-bearing composite panels, materials, and products made by surrounding with a long fiber and/or fiber cloth reinforced polyurethane resin, an assembly containing one or more load-bearing members, graphene, a structural polyurethane/resin sandwich composite and/or spider silk protein fiber-cloth-continuous fibers. The composite structures can provide stronger, lighter-weight structural items such as vehicle floor and body panels, bullet-proof anti-ballistic panel products, vehicle bullet-proof anti-ballistic body panel structures and floors, bullet-proof vests, vehicle chassis, monocoque chassis, motor homes chassis-bodies, fuselage floors and frames for aircraft and/or UAV's, bicycle and motorcycle frames, wind turbine blades frames and structures, ship or boat haul body structures, shipment containers, pre-fabricated walls of buildings, train structure body or floor panels, solar panel supports, battery housings, mobile home walls, roof modules, truck beds, and truck trailer floors. Such composite panels, materials, and products can also be utilized in artificial organs, ligaments or tendons, artificial disc vertebrae, ropes, and 3D printing parts.

The invention provides an isolated major ampullate spidroin protein, which consists of from 150 to 420 amino acid residues and is defined by the formula KEP-CT. KEP is a repetitive, N-terminally derived protein fragment having from 80 to 300 amino acid residues. CT is a C-terminally derived protein fragment having from 70 to 120 amino acid residues. The invention further provides an isolated fusion protein consisting of a first protein fragment, which is a major ampullate spidroin protein, and a second protein fragment comprising a fusion partner and a cleavage agent recognition site. The first protein fragment is coupled via said cleavage agent recognition site to the fusion partner. The invention also provides a method of producing a major ampullate spidroin protein and polymers thereof.

A solution-dyed protein fiber of the present invention includes 0-100 mass % of silk fibroin and 100-0 mass % of a polypeptide derived from spider silk proteins when the protein fiber is assumed to be 100 mass %, wherein the solution-dyed protein fiber contains a solution-dyeing colorant. The fiber is obtained by: dissolving or dispersing a solution-dyeing colorant in a solvent used for a spinning solution or in dimethyl sulfoxide, thereby preparing a coloring liquid; adding a solvent to the coloring liquid in an amount necessary for a spinning solution; adding and dissolving protein powder into the solvent, thereby preparing a spinning solution; and subjecting the spinning solution to wet spinning or dry-wet spinning. Thereby, the present invention provides a low-cost solution-dyed protein fiber in which a solution-dyeing colorant is dispersed uniformly and that can exhibit bright color tone, and a method for producing the same.

Transgenic silkworms comprising at least one nucleic acid encoding a chimeric silk polypeptide comprising one or more spider silk elasticity and strength motifs are disclosed. Expression cassettes comprising nucleic acids encoding a variety of chimeric spider silk polypeptides (Spider 2, Spider 4, Spider 6, Spider 8) are also disclosed. A piggyBac vector system is used to incorporate nucleic acids encoding chimeric spider silk polypeptides into the mutant silkworms to generate stable transgenic silkworms. Chimeric silk fibers having improved tensile strength and elasticity characteristics compared to native silkworm silk fibers are also provided. The transgenic silkworms greatly facilitate the commercial production of chimeric silk fibers suitable for use in a wide variety of medical and industrial applications.

The present invention relates to a spider silk crystal protein gene synthesized by plant preferance codon, its expression carrier, the plant cells transformed from it, and the transgenic plant and its descendant. Said gene can be effectively expressed in fibrous plant and the obtained protein moleculae can be combined with the cellulose of plant, so improving the strength of plant fibres.

The invention relates to the field of tissue engineering, in particular to a preparation method of a composite nanometer fiber small-diameter intravascular tissue engineering stent material. The technical scheme is achieved as follows: formic acid is used as a common solvent; RGD-recombinant spider silk protein, polycaprolactone and chitosan are mixed in a mass ratio of 1:8:1 according to the requirements of an intravascular stent to prepare a spinning solution with the concentration of 20-30 percent (w/v); electrospinning process parameters are as follows: the diameter of a cross section of acylindrical rotating shaft is 3-6 mm, the rotating speed is 1,500-3,000rpm, the curing distance is 15-30 cm, the voltage is 50-150kV, the extrusion speed is 3-7ml/h and the temperature is 45-50 DEG C. The small-diameter intravascular stent has favorable cell adhesion-promoting capability, anticoagulant property and mechanical property for resisting physiological environment and can be clinicallyapplied as a small-diameter intravascular tissue engineering stent.

The invention relates to a bionic spider silk-like polymer fiber with a water collecting characteristic and a preparation method thereof, belonging to the field of chemical bionic technology. The bionic spider silk-like polymer fiber with the water collecting characteristic comprises, by weight, 100 parts of a fiber matrix polymer and 1 to 50 parts of spray-dried powdered rubber. The filament of the polymer fiber has a spider silk-like microstructure with spindle knot units and joint units distributed at intervals, wherein each spindle knot unit has a radial height of 10 to 300 [mu]m and an axial length of 10 to 1000 [mu]m; the diameters of the joint units are in a range of 5 to 200 [mu]m; and the lengths of the joint units are in a range of 10 to 5000 [mu]m. According to the invention, the method can be implemented by using traditional spinning equipment without any extra additional equipment, and is simple and stable in process and capable of realizing continuous large-scale preparation. The prepared fiber with a bionic spider silk structure in the invention has low cost and good stability.

The present invention relates to the fields of molecular biology and plant biology. Specifically, the invention is directed to the methods for expressing spider silk proteins in plants and the synthesis and purification of spider silk proteins therefrom.