29 results about "Spider silk" patented technology

The application discloses a kind of imitative spider silk cellulose gel fibers and its preparation method and application, method includes: using DMAc to activate cellulose powder and obtain activated cellulose powder;Activated cellulose powder is added in DMAc / LiCl solvent and dissolved, and obtain cellulose solution;Using microfluidic equipment, cellulose solution is injected into deionized water and solidified, and washing is obtained cellulose gel fiber;Cellulose gel fiber is soaked in PVA-PAA precursor liquid, and then placed under ultraviolet lamp irradiation, then freeze and thaw, and obtain cellulose / PVA / PAA hydrogel fiber;Cellulose / PVA / PAA hydrogel fiber is pre-stretched, then soaked in CuSO4 solution, and then washed with deionized water after soaking, and obtain imitative spider silk cellulose gel fiber.The imitative spider silk cellulose gel fiber provided by the application has strong toughness, flexible conductivity and environmental resistance.

A recombinant spider silk protein, consisting of no more than 800 amino acids, comprising a set of domains arranged according to the formula (NT)-REP-CT, wherein: the optional NT-domain, if present, comprises a sequence of 100 to 160 amino-acid residues derived from the N-terminal domain of a spider silk protein; the REP-domain comprises a sequence of 30 to 600 amino acid residues derived from the repetitive segment of a spider silk protein; and the CT-domain comprises a sequence of 70 to 120 amino acid residues derived from the C-terminal domain of a spider silk protein selected from: a sequence of 72 to 110 amino acid residues derived from the C-terminal domain of a spider silk protein, wherein the sequence comprises at least 7 residues independently selected from K, R, E and D; a sequence having at least 85% identity to SEQ ID NO: 15 or any one of SEQ ID NOs: 62-65 or 67-73; and a sequence having at least 70% identity to SEQ ID NOs: 64 or any one of SEQ ID NOs: 62-65 or 67-73, wherein the sequence comprises at least 7 residues independently selected from K, R, E and D.

A plurality of methods and apparatus for managing deficiencies of the meibomian gland lipid production and delivery, including wearable fabrics configured with a matrix of micro- and nano-electromechanical materials, or composite fibres of yarn that are substantially made from carbon nanotubes, graphene, spider silk, natural silk, denim, cotton, metals, or combination thereof. Other methods and apparatus include infrared and ultrasound energy sources within a kit aimed at personalised therapies for improving overall health of the eyelids, while providing for adjunctive relief for associated dry eye symptoms. The management kit may comprise a wearable fabric or spectacle frame configured with infrared emitters and sensors, a handheld imaging device and software containing dosage and administration information coupled with information on improving eyelid hygiene which is reviewed and periodically updated using user-specific information.

The application discloses a kind of biomimetic water-collecting and compacting device co-located in corn seeding site, belong to the technical field of agricultural machinery equipment, the device includes biomimetic compacting wheel, compacting wheel side counterweight and floating buffer mechanism.Biomimetic compacting wheel is circumferentially provided with protrusions, and the surface of the protrusions is arranged with water-collecting microstructure imitating spider silk spindle node-connecting fiber, which relies on Laplace differential pressure to direct water collection.By matching the seed falling time and the rotation stroke of the biomimetic compacting wheel, the protrusions can accurately point to the seeds for compacting;The floating buffer mechanism can adapt to the ups and downs of the surface and adjust the compacting load.The device simultaneously completes accurate compacting and water-collecting soil conservation in one operation, improving the soil moisture content and the corn emergence rate.

The invention discloses a preparation method of anti-crack and anti-freezing fiber composite concrete, and relates to the field of concrete. The preparation method comprises the following steps: immersing bionic spider silk into a microbial mineralization solution, adding a photoresponse nano material, and adjusting illumination, so that calcium carbonate crystals are arranged in a honeycomb shape on the surface of the spider silk, bionic spider silk fibers and microbial mineralization layers are alternately deposited, and a'fiber-mineralization layer-fiber 'sandwich structure is constructed. The structure cooperates with a spider silk spiral and honeycomb mineralization layer, the concrete interface strength is greatly improved, shrinkage cracks are effectively inhibited, microorganisms are activated and mineralized in the cracks to repair the cracks, and the concrete is endowed with high crack resistance and self-repairing capacity. Meanwhile, the self-made nano-composite fiber takes polyvinyl alcohol and ethylene glycol copolymer as main materials, polylactic acid micro-cavities containing anhydrous calcium chloride are connected to two ends of the self-made nano-composite fiber, and the freezing resistance of the concrete is improved by the hydrophilicity of the polyvinyl alcohol and the heat release of the anhydrous calcium chloride. The anti-cracking and anti-freezing fiber composite concrete prepared by the invention has the effects of high cracking resistance and high freezing resistance.

This invention discloses a self-contained conical microchannel and its design method. The microchannel includes a conical channel with a biomimetic fishtail structure and a biomimetic spider silk structure on its surface. The biomimetic fishtail structure is a fishtail-shaped groove formed by bending the base of a triangle with a consistent orientation inward. The biomimetic spider silk structure is a spider silk-shaped groove composed of hemispherical and conical channels. Multiple biomimetic fishtail structures are arranged in an array, and multiple biomimetic spider silk structures are arranged in an array, alternating between the fishtail and spider silk arrays. This invention combines the biomimetic fishtail and spider silk structures on the surface of the conical channel. The fishtail structure enables self-collection of liquid within the conical microchannel, while the spider silk structure enables rapid self-transportation of liquid. This technology can be applied to micro-drug delivery without external force, microfluidic devices, and friction interfaces.

The invention discloses a spider silk collecting device and process, and particularly relates to the technical field of spider silk, the spider silk collecting device comprises a telescopic rod composed of an expansion sleeve and two long rods, the head of the telescopic rod is provided with an installation assembly, the front portion of the installation assembly is provided with flannelette fixed through an opening clamp, and the surface of the flannelette is provided with three-dimensional fluff. And collecting the whole cobweb by means of the three-dimensional fluff. According to the spider silk collecting device and technology, in the storage technology, the flannelette attached with spider webs is fixed to the foaming plates through insect needles, spider silk stress and adhesion caused by flannelette bending are effectively avoided, and through the small foaming plates fixed to the four corners of the foaming plates, when a plurality of foaming plates carrying spider silk are placed in a carton, the spider silk collecting effect is good, and the spider silk collecting effect is good. Stable intervals are formed, and extrusion damage during transportation or storage is prevented.

The application provides a kidney-tonifying and anti-fatigue polypeptide composition and a preparation method thereof, belongs to the technical field of medicinal biological peptide complexes, and the composition comprises oyster polypeptide, holothurian polypeptide, spider silk and blue ears grass-pentamerous fruit composite extract, cistanche-dendrobium officinale composite extract, ginseng extract and deer penis extract; the oyster polypeptide is a common enzymolysis product of oyster meat by trypsin and bovine chymotrypsin; the holothurian polypeptide is a common enzymolysis product of holothurian body wall by alkaline protease, collagenase type II and subtilisin; the spider silk and blue ears grass-pentamerous fruit composite extract is a product obtained by the following steps: subjecting spider silk and blue ears grass and pentamerous fruit to enzymolysis, extracting with an ethanol aqueous solution, and purifying with a SA sulfonic acid silica gel strong cation exchange column; the cistanche-dendrobium officinale composite extract is a product obtained by the following steps: subjecting cistanche and dendrobium officinale to enzymolysis, extracting with an ethanol aqueous solution, and purifying with a phenylboronic acid affinity column-polyamide column series connection column; and the composition realizes the synergistic effect of kidney tonification and anti-fatigue.

The invention discloses an environment-friendly silk fabric low-temperature plasma anti-wrinkle finishing process, and particularly relates to the technical field of biopolymer engineering. The process sequentially comprises the steps of pre-cleaning, low-temperature plasma activation, polyproline solution impregnation, roll compacting and pre-drying, acetylation treatment, neutralization and soaping, dehydration and drying and the like. Performing micro-etching and activation on the surface of the silk through oxygen low-temperature plasma to increase reaction sites; constructing a biological cross-linked network imitating the elasticity of spider silk on the surface of the fiber by utilizing polyproline with a II-type helical structure; unreacted amino groups are sealed through acetylation treatment, so that the hydrophobicity and the network stability are enhanced. The process does not need to use formaldehyde resin, keeps high breaking strength retention rate and washable stability while realizing excellent wrinkle resistance, has the comprehensive advantages of environmental protection and excellent durability, and is suitable for wrinkle-resistant function finishing of high-end silk products.

The utility model belongs to the field of agricultural machinery, and particularly relates to a bionic ditching and pressing device which comprises a ditching device and a seed furrow pressing device. The working section of a seed furrow pressing wheel in the seed furrow pressing device is a spider silk-like spindle knot curve, the equation of the spider silk-like spindle knot curve is y = 0.0397 * 2-0.9936 x + 6.3198, the value range of x is 0-25 mm, and the value range of y is 0-6.3198 mm. The device can realize the functions of ditching, seed furrow pressing and furrow shape construction, after seed furrow pressing, macropores of soil at the bottom of a seed furrow are reduced, the proportion of small pores is increased, the capillary effect of soil moisture is enhanced, and the moisture retention capacity of a seedbed is improved; a spider silk-like spindle knot curve ditch bottom formed by the bionic working section of the seed ditch press wheel has a certain water collecting effect; the formed seedbed environment is good in soil moisture content, corn seed germination and seedling emergence are facilitated, and the problem of seedbed moisture loss caused by traditional V-shaped seed ditches is solved.

The invention relates to the technical field of biomimetic materials, polymer composite materials and polymer fibers, and discloses a preparation method of biomimetic assembled high-performance artificial spider silk fibers, which comprises the following steps: preparing primary gel fibers containing rigid network nodes and flexible polymer chains; stretching the primary gel fiber in a closed environment in which the temperature and the solvent vapor pressure are controlled at the same time; according to the environment, solvent molecules preferentially swell the flexible polymer chain, the rigid network nodes are kept stable, and the applied drawing force drives the flexible polymer chain to slide and axially orientate among the rigid network nodes. According to the method, the specific stretching environment is constructed, internal physical conflicts of stretching gain and structural damage are remodeled into a synergistic process, the situation that microdefects are generated at a rigid-flexible interface by stretching force is avoided, and a bionic rigid-flexible composite structure which is difficult to achieve in the prior art and has high integrity is obtained for final fibers.

Cosmeceutical compositions comprising a major ampullate spidroin protein (MaSp)-based fiber are disclosed. Articles comprising the compositions are further disclosed.

The invention discloses a spider silk-like hybrid polymer fiber and a preparation method thereof, and belongs to the field of synthetic fibers, and the preparation method comprises the following steps: dissolving, heating and stirring a carboxyl-containing polymer until the carboxyl-containing polymer is completely swelled, and adding an acid solution for adjustment to obtain polymer gel; adding an amino-containing polymer into the polymer gel, heating and uniformly stirring to obtain hybrid gel, and centrifuging the hybrid gel to obtain a hybrid gel spinning precursor stock solution; extruding the hybrid gel spinning precursor stock solution into an anti-solvent coagulating bath, collecting fibers, and obtaining hybrid polymer fibers after moisture absorption is balanced; and carrying out pre-stretching and moisture absorption balance to obtain the spider silk-like hybrid polymer fibers with different uniaxial orientation and crystallinity structures. According to the invention, continuous preparation of the fiber is realized by combining the high-molecular polymer with anti-solvent induced phase separation and pre-stretching strain programming, the method is simple, the raw material source is environment-friendly, and the prepared fiber has comprehensive characteristics similar to spider silk.

The invention provides a preparation method of spider protein synthetic leather, which comprises the following steps: S10, preparing surface layer slurry: selecting spider protein powder with the average particle size D50 of 1.0-10.0 microns, dispersing the spider protein powder in a polar organic solvent for pre-infiltration treatment to obtain suspension liquid, and drying the suspension liquid to obtain the surface layer slurry; then mixing the suspension liquid with a polyurethane surface layer resin solution, and uniformly dispersing to prepare surface layer slurry containing spider protein powder; s20, preparing inner-layer slurry: preparing polyurethane inner-layer slurry which does not contain arachnoid protein powder; s30, dry molding: coating release paper with the surface layer slurry, drying the surface layer slurry to form a surface layer, and controlling the ratio of the dry film thickness of the surface layer to the average particle size D50 of the spider protein powder to be (5: 1)-(10: 1); and then coating the surface layer with the inner layer slurry, drying to form an inner layer, attaching base cloth to the inner layer, and curing and stripping to obtain the spider protein synthetic leather. The synthetic leather prepared by the preparation method has good mechanical properties, good breathable effect and excellent low-temperature folding resistance.

The invention relates to the technical field of leather materials, and particularly discloses wear-resistant leather with an enhanced protein bionic structure and a preparation method of the wear-resistant leather. The wear-resistant leather sequentially comprises a multi-stage bionic wear-resistant surface layer, a protein bionic core layer and base cloth from top to bottom, pangolin scale macroscopic ribs and sharkskin microcosmic grooves are re-carved on the multi-stage bionic wear-resistant surface layer, and a nano hard-phase filler is compounded; the protein bionic core layer adopts a spider silk multistage fiber structure and a shell hard phase embedding mechanism, and a dynamic cross-linked network is introduced; and the transition layer and the dynamic cross-linking bonding layer realize interlayer stable combination and micro-damage self-repairing. The preparation method comprises the steps of raw material pretreatment, raw material preparation of each layer, step-by-step forming, composite curing and the like. Through multistage collaborative bionic and dynamic crosslinking design, the problems that traditional protein-based leather is poor in wear resistance and insufficient in long-term effect are solved, meanwhile, the skin-friendly and environment-friendly advantages are reserved, the wear resistance, the tearing strength and the interlayer binding force are remarkably improved, and the protein-based leather is suitable for high-end shoes, automobile interiors and other scenes.

The invention belongs to the technical field of material science, and particularly provides a preparation method of fiber containing spider silk protein, which comprises the following steps: 1) stirring and mixing 20-50 parts by weight of spider silk protein hydrolysate, 5-10 parts by weight of modified protamine, 20-50 parts by weight of polylactic acid and 20-50 parts by weight of ethanol to form a spinning solution; and (2) spraying the spinning solution obtained in the step (1) into bath lotion by adopting an electrostatic spinning technology, and then performing silk guiding, heating and winding forming to obtain the fiber containing the spider silk protein. Meanwhile, the invention also provides the fiber containing the spider silk protein prepared by the method, and application of the fiber in biomedical materials. The invention mainly solves the problems of single function and insufficient comprehensive performance of the existing fiber containing spider silk protein.

The application discloses a spider-silk-microstructure-shaped-memory polyimide composite material and a preparation method and application thereof, and the composite material is designed by coupling a spider-silk-microstructure-shaped-memory polyimide, a flame retardant and an electromagnetic shielding two-dimensional intercalation compound.The conductive polyaniline is intercalated with a transition metal carbon / nitride to prepare a Ti3C2T x @PANI intercalation compound, and then the SMPI is compounded to enhance the mechanical strength and electromagnetic shielding property of the spider-silk-microstructure-shaped resin matrix, and when the thickness of the SMPI composite material is 100 microns, the electromagnetic shielding efficiency of the SMPI composite material exceeds 25 dB in the X wave band, and more than 99% of the incident microwaves are effectively shielded.In addition, the flame retardant grade of the composite material reaches V0 level, the limiting oxygen index is 39.1%, the composite material also has high-temperature resistance and shape memory characteristics, the shape fixing rate is 96%, and the shape recovery rate is 97%.The composite material can eliminate electromagnetic interference, is flame-retardant, and can also autonomously deform, and is applied to the fields of self-adapting deformation, thermal management electromagnetic shielding devices and intelligent wearable devices.

The present disclosure relates to methods for isolating and purifying synthetic block copolymer proteins using solvent and high shear microfluidization techniques.

The invention provides an enterokinase light chain recombinant protein and application and a method for efficient soluble expression of a recombinant enterokinase light chain, the enterokinase light chain recombinant protein is composed of more than two fusion proteins AgSg or mutants thereof and an enterokinase light chain or mutants thereof, the amino acid sequence of the fusion proteins AgSg is shown as SEQ ID NO.3, and the amino acid sequence of the fusion proteins AgSg is shown as SEQ ID NO.3. The amino acid sequence of the enterokinase light chain is as shown in SEQ ID NO. 1; according to the enterokinase light chain recombinant protein, a specific amount of fusion protein AgSg derived from the N end of spider silk sericin is fused with an enterokinase light chain or a mutant thereof in a series connection manner, so that the expression quantity of the enterokinase light chain or / and the mutant can be remarkably improved, and the enterokinase light chain or / and the mutant can be expressed in a soluble form; the defects that a traditional escherichia coli system is low in expression quantity and expression and purification are difficult in an inclusion body form are overcome, and the method can be applied to production of high-efficiency soluble enterokinase light chains.

1. Name of the designed product: transmitter (small spider silk). 2. Use of the designed product: for toys. 3. Design points of the designed product: in shape. 4. Picture or photo best indicating the design points: perspective view.

The present invention relates to recombinant spidroin bioconjugates (chemically modified engineered spider silk proteins). More particularly, the invention relates to recombinant spidroin bioconjugates with poly-ethyleneglycol polymers and their use in regenerative medicine.

Described are spider silk articles of wear. The article of wear, which may be apparel or shoes, includes an inner surface, wherein the inner surface includes an area including spider silk. The spider silk of the article of wear may be arranged to contact the skin of a wearer when the article of wear is worn.

The invention discloses a preparation method of a dual bionic wettability patterned fog collection surface structure, and belongs to the field of atmospheric water collection and functional surface preparation. Based on a double bionic idea of cactus and spider silk, the preparation of the fog collecting surface structure is realized by combining laser ablation and surface modification technologies. The method comprises the following steps: firstly, carrying out primary laser ablation on an aluminum sheet to obtain a super-hydrophilic aluminum sheet substrate; carrying out ultrasonic cleaning, and immersing into a fluorosilane alcohol solution to carry out low surface energy modification treatment; then, the modified aluminum sheet is moved into a drying box to be dried, and the super-hydrophobic aluminum sheet is obtained. And a hydrophilic area with a preset pattern is constructed on the super-hydrophobic aluminum sheet through secondary laser ablation, a rectangular through hole structure is constructed through continuous laser ablation, and the fog collecting surface structure is obtained. The method is simple in process step, the obtained surface has excellent fog water collecting performance, and the method is suitable for large-scale production.

The application provides an unpowered bottom layer floating lander based on bionic principle, which comprises a floating body, a lander body, a plurality of hanging devices, a flexible bearing cable and a plurality of ballast; the floating body is connected with the lander body through the hanging devices; the flexible bearing cable is connected between the floating body and the lander body in a slack manner; the lander body comprises a controller, a battery connected with the controller and a plurality of throw-off devices; the throw-off devices are connected with the ballast; and the controller is further connected with the hanging devices. The unpowered bottom layer floating lander based on bionic principle changes the rigid connection of the traditional lander into the flexible cable connection based on the bionic principle of spider silk flight; and the propulsion of the sea current to the flexible cable is utilized to realize the unpowered cruising of the lander near the bottom.

A recombinant bacterium having the ability to / express MaSP-based proteins, nucleic acids encoding same and method for the preparation of synthetic dragline spider silk are provided.

The invention discloses a PET enzyme-spider silk protease recombinant protein and application thereof in degradation of PET microplastics, the recombinant protein is obtained by connecting a spider silk protein R gene to a C terminal of a PETase gene through a seamless cloning technology, and after a PET digestive enzyme is combined with spider silk protease, the degradation efficiency of the PET digestive enzyme in a sewage water body can be improved; according to the present invention, the defects that the PET material micro-plastic has characteristics of large dispersion area, low enzyme contact probability and difficult degradation are overcome, and the SDS-PAGE analysis, the p-NP determination method, the high performance liquid chromatography and other technical means prove that the recombinant protein is more effective than the single PETase in the PET plastic degradation aspect. The method provided by the invention not only solves the problem of low degradation efficiency of the PET micro-plastic in the sewage water body, but also provides an innovative biochemical solution for environmental protection and plastic pollution treatment, and has important practical value and wide application prospect.

The invention relates to the field of communication, in particular to a bending-resistant super-flexible cable with high shielding effectiveness, which comprises a conductor, an insulating layer wrapped on the conductor, an isolating layer wrapped on the insulating layer, an inner sheath wrapped on the isolating layer, a shielding layer wrapped on the inner sheath and an outer sheath wrapped on a weaving layer, and the isolating layer is formed by weaving artificial spider threads. The artificial spider threads have high strength and toughness, and the conductor and the insulating layer are wrapped by the artificial spider threads, so that the overall strength is improved, and the tensile property and the bending resistance of the cable are improved.