49 results about "Spider silk" patented technology

The invention provides a bio-based adhesive and a preparation method thereof. Inspired by a spider thread structure, biomass adhesive interface enhancement based on a tannic acid adhesion group is combined with construction of a differential phase separation structure by biomass-induced polylipoic acid depolymerization, and a brand new strategy of adhesion-cohesion synergistic enhancement of the adhesive strength of the adhesive is realized. According to the invention, tannic acid and carboxymethyl chitosan are introduced into a lipoic acid-based adhesive, so that the purpose of synergistically enhancing the bonding performance of the adhesive through adhesion-cohesion is achieved, and the defects of weak cohesion, low bonding strength, poor water resistance, poor moisture resistance and the like of the conventional biomass adhesive are overcome. Benefited from a new strategy of adhesion-cohesion synergistic enhancement, the biomass adhesive has good adhesive strength in various environments and materials. In addition, the biomass adhesive also has the characteristics of biological safety, antibacterial and mildew-proof properties and recoverability, so that the product has wider application and potential compared with the previous product, and has a good application prospect.

The invention discloses a recombinant protein and application thereof in preparation of sodium alginate fibers. The recombinant protein is obtained by fusion expression of a functional protein and at least one alginic acid binding domain protein. The amino acid sequence of the alginic acid binding domain protein is as shown in at least one of SEQ ID No. 1, SEQ ID No. 2 and SEQ ID No. 3. The recombinant spider silk protein with the alginic acid CBM region can exert the material science characteristics in a sodium alginate-calcium ion system, the mechanical property of alginic acid fiber is greatly enhanced, and meanwhile, the biological activity of protein can be provided for an alginate fiber system.

Cosmeceutical compositions comprising a major ampullate spidroin protein (MaSp)-based fiber are disclosed. Articles comprising the compositions are further disclosed.

The application discloses a kind of imitative spider silk cellulose gel fibers and its preparation method and application, method includes: using DMAc to activate cellulose powder and obtain activated cellulose powder;Activated cellulose powder is added in DMAc / LiCl solvent and dissolved, and obtain cellulose solution;Using microfluidic equipment, cellulose solution is injected into deionized water and solidified, and washing is obtained cellulose gel fiber;Cellulose gel fiber is soaked in PVA-PAA precursor liquid, and then placed under ultraviolet lamp irradiation, then freeze and thaw, and obtain cellulose / PVA / PAA hydrogel fiber;Cellulose / PVA / PAA hydrogel fiber is pre-stretched, then soaked in CuSO4 solution, and then washed with deionized water after soaking, and obtain imitative spider silk cellulose gel fiber.The imitative spider silk cellulose gel fiber provided by the application has strong toughness, flexible conductivity and environmental resistance.

Compared with the prior art, the method for extracting the spider silk fibroin has the advantage that the content of the spider silk fibroin can be remarkably increased.

A recombinant spider silk protein comprises an NT domain, a REP domain and a CT domain. The REP domain comprises a set of domains according to the formula pA1-pG-pA2. pG represents a glycine-rich domain and pA1 and pA2 represent alanine-rich domains. One of pA1 and pA2 is a poly-alanine domain and the other of pA1 and pA2 is a poly-alanine domain having every third or fourth alanine residue replaced by an isoleucine residue or a valine residue. Spider silk spun from the recombinant spider silk proteins of the invention have improved mechanical properties.

Compared with the prior art, the method for extracting the spider silk fibroin has the advantage that the content of the spider silk fibroin can be remarkably increased.

The invention provides a cement-based material with a spider silk-like microstructure and a preparation method of the cement-based material, and belongs to the technical field of cement-based materials and preparation of the cement-based materials. The cement-based material comprises the following raw materials: double-component cement, reinforced fibers, mineral whiskers and an additive, the two-component cement comprises silicate cement and sulphoaluminate cement. The expansion effect generated by the hydration reaction of anhydrous calcium sulphoaluminate in the double-component cement not only counteracts the partial shrinkage effect generated by the hydration of Portland cement clinker minerals, but also extrudes the reinforced fibers, so that the binding force between the fibers and a cement matrix is increased. Besides, a secondary frame formed by the generated ettringite raphioid crystals and the added mineral whiskers is combined with a main body frame of the reinforced fibers to form a spider-screen-frame-imitating toughening structure, so that the strength of the material can be improved, and the toughness of the material is also improved.

The invention discloses a composition containing spider silk hydrolysate. The composition is prepared from the following raw materials in parts by weight: 25-35 parts of spider silk hydrolysate, 15-20 parts of cinnamon leaf residue extracting solution, 2-6 parts of lotus extracting solution, 3-7 parts of stearyl alcohol, 5-9 parts of glycerol, 2-6 parts of palmitoyl tripeptide and 14 parts of stearyl glucoside. The invention further provides application of the composition containing the spider silk hydrolysate in preparation of anti-aging drugs or skin care products. According to the composition of the spider silk hydrolysate, the anti-aging effect is improved, the added stearyl alcohol, glycerol, palmitoyl tripeptide and stearyl glucoside are used as auxiliary materials to be applied to anti-aging drugs or skin care products, the good anti-aging curative effect is achieved, the increase rate of the skin moisture content of the products can be improved due to the addition of the spider silk hydrolysate, and the anti-aging effect of the products is improved. And meanwhile, the anti-aging effect of the product can be stabilized, and the raw materials are matched with one another, so that a remarkable improvement effect is achieved.

A recombinant spider silk protein, consisting of no more than 800 amino acids, comprising a set of domains arranged according to the formula (NT)-REP-CT, wherein: the optional NT-domain, if present, comprises a sequence of 100 to 160 amino-acid residues derived from the N-terminal domain of a spider silk protein; the REP-domain comprises a sequence of 30 to 600 amino acid residues derived from the repetitive segment of a spider silk protein; and the CT-domain comprises a sequence of 70 to 120 amino acid residues derived from the C-terminal domain of a spider silk protein selected from: a sequence of 72 to 110 amino acid residues derived from the C-terminal domain of a spider silk protein, wherein the sequence comprises at least 7 residues independently selected from K, R, E and D; a sequence having at least 85% identity to SEQ ID NO: 15 or any one of SEQ ID NOs: 62-65 or 67-73; and a sequence having at least 70% identity to SEQ ID NOs: 64 or any one of SEQ ID NOs: 62-65 or 67-73, wherein the sequence comprises at least 7 residues independently selected from K, R, E and D.

A plurality of methods and apparatus for managing deficiencies of the meibomian gland lipid production and delivery, including wearable fabrics configured with a matrix of micro- and nano-electromechanical materials, or composite fibres of yarn that are substantially made from carbon nanotubes, graphene, spider silk, natural silk, denim, cotton, metals, or combination thereof. Other methods and apparatus include infrared and ultrasound energy sources within a kit aimed at personalised therapies for improving overall health of the eyelids, while providing for adjunctive relief for associated dry eye symptoms. The management kit may comprise a wearable fabric or spectacle frame configured with infrared emitters and sensors, a handheld imaging device and software containing dosage and administration information coupled with information on improving eyelid hygiene which is reviewed and periodically updated using user-specific information.

The application discloses a kind of biomimetic water-collecting and compacting device co-located in corn seeding site, belong to the technical field of agricultural machinery equipment, the device includes biomimetic compacting wheel, compacting wheel side counterweight and floating buffer mechanism.Biomimetic compacting wheel is circumferentially provided with protrusions, and the surface of the protrusions is arranged with water-collecting microstructure imitating spider silk spindle node-connecting fiber, which relies on Laplace differential pressure to direct water collection.By matching the seed falling time and the rotation stroke of the biomimetic compacting wheel, the protrusions can accurately point to the seeds for compacting;The floating buffer mechanism can adapt to the ups and downs of the surface and adjust the compacting load.The device simultaneously completes accurate compacting and water-collecting soil conservation in one operation, improving the soil moisture content and the corn emergence rate.

The invention discloses a costume fabric containing spider silk, which is characterized in that modified spider silk is used as first warp and weft spinning yarn, antibacterial cotton thread is used as second warp and weft spinning yarn, and then the first warp and weft spinning yarn and the second warp and weft spinning yarn are interlaced and woven in the warp and weft direction to obtain the costume fabric containing spider silk. In addition, the invention further provides a preparation method of the garment fabric containing the spider silk. The prepared fabric containing spider silk is simple in preparation method and very excellent in breaking strength, tearing strength and puncture resistance, and the fabric has very good antibacterial property on staphylococcus aureus and escherichia coli after being subjected to aging treatment or saline water treatment and is also very excellent in antibacterial stability.

The invention discloses a preparation method of anti-crack and anti-freezing fiber composite concrete, and relates to the field of concrete. The preparation method comprises the following steps: immersing bionic spider silk into a microbial mineralization solution, adding a photoresponse nano material, and adjusting illumination, so that calcium carbonate crystals are arranged in a honeycomb shape on the surface of the spider silk, bionic spider silk fibers and microbial mineralization layers are alternately deposited, and a'fiber-mineralization layer-fiber 'sandwich structure is constructed. The structure cooperates with a spider silk spiral and honeycomb mineralization layer, the concrete interface strength is greatly improved, shrinkage cracks are effectively inhibited, microorganisms are activated and mineralized in the cracks to repair the cracks, and the concrete is endowed with high crack resistance and self-repairing capacity. Meanwhile, the self-made nano-composite fiber takes polyvinyl alcohol and ethylene glycol copolymer as main materials, polylactic acid micro-cavities containing anhydrous calcium chloride are connected to two ends of the self-made nano-composite fiber, and the freezing resistance of the concrete is improved by the hydrophilicity of the polyvinyl alcohol and the heat release of the anhydrous calcium chloride. The anti-cracking and anti-freezing fiber composite concrete prepared by the invention has the effects of high cracking resistance and high freezing resistance.

This invention discloses a self-contained conical microchannel and its design method. The microchannel includes a conical channel with a biomimetic fishtail structure and a biomimetic spider silk structure on its surface. The biomimetic fishtail structure is a fishtail-shaped groove formed by bending the base of a triangle with a consistent orientation inward. The biomimetic spider silk structure is a spider silk-shaped groove composed of hemispherical and conical channels. Multiple biomimetic fishtail structures are arranged in an array, and multiple biomimetic spider silk structures are arranged in an array, alternating between the fishtail and spider silk arrays. This invention combines the biomimetic fishtail and spider silk structures on the surface of the conical channel. The fishtail structure enables self-collection of liquid within the conical microchannel, while the spider silk structure enables rapid self-transportation of liquid. This technology can be applied to micro-drug delivery without external force, microfluidic devices, and friction interfaces.

The invention discloses anti-cracking and anti-freezing fiber composite concrete and a preparation method thereof, and relates to the field of concrete. The preparation method comprises the following steps: immersing bionic spider silk into a microbial mineralization solution, adding a photoresponse nano material, and adjusting illumination, so that calcium carbonate crystals are arranged in a honeycomb shape on the surface of the spider silk, bionic spider silk fibers and microbial mineralization layers are alternately deposited, and a'fiber-mineralization layer-fiber 'sandwich structure is constructed. The structure cooperates with a spider silk spiral and honeycomb mineralization layer, the concrete interface strength is greatly improved, shrinkage cracks are effectively inhibited, microorganisms are activated and mineralized in the cracks to repair the cracks, and the concrete is endowed with high crack resistance and self-repairing capacity. Meanwhile, the self-made nano-composite fiber takes polyvinyl alcohol and ethylene glycol copolymer as main materials, polylactic acid micro-cavities containing anhydrous calcium chloride are connected to two ends of the self-made nano-composite fiber, and the freezing resistance of the concrete is improved by the hydrophilicity of the polyvinyl alcohol and the heat release of the anhydrous calcium chloride. The anti-cracking and anti-freezing fiber composite concrete prepared by the invention has the effects of high cracking resistance and high freezing resistance.

The invention discloses a spider silk collecting device and process, and particularly relates to the technical field of spider silk, the spider silk collecting device comprises a telescopic rod composed of an expansion sleeve and two long rods, the head of the telescopic rod is provided with an installation assembly, the front portion of the installation assembly is provided with flannelette fixed through an opening clamp, and the surface of the flannelette is provided with three-dimensional fluff. And collecting the whole cobweb by means of the three-dimensional fluff. According to the spider silk collecting device and technology, in the storage technology, the flannelette attached with spider webs is fixed to the foaming plates through insect needles, spider silk stress and adhesion caused by flannelette bending are effectively avoided, and through the small foaming plates fixed to the four corners of the foaming plates, when a plurality of foaming plates carrying spider silk are placed in a carton, the spider silk collecting effect is good, and the spider silk collecting effect is good. Stable intervals are formed, and extrusion damage during transportation or storage is prevented.

The application provides a kidney-tonifying and anti-fatigue polypeptide composition and a preparation method thereof, belongs to the technical field of medicinal biological peptide complexes, and the composition comprises oyster polypeptide, holothurian polypeptide, spider silk and blue ears grass-pentamerous fruit composite extract, cistanche-dendrobium officinale composite extract, ginseng extract and deer penis extract; the oyster polypeptide is a common enzymolysis product of oyster meat by trypsin and bovine chymotrypsin; the holothurian polypeptide is a common enzymolysis product of holothurian body wall by alkaline protease, collagenase type II and subtilisin; the spider silk and blue ears grass-pentamerous fruit composite extract is a product obtained by the following steps: subjecting spider silk and blue ears grass and pentamerous fruit to enzymolysis, extracting with an ethanol aqueous solution, and purifying with a SA sulfonic acid silica gel strong cation exchange column; the cistanche-dendrobium officinale composite extract is a product obtained by the following steps: subjecting cistanche and dendrobium officinale to enzymolysis, extracting with an ethanol aqueous solution, and purifying with a phenylboronic acid affinity column-polyamide column series connection column; and the composition realizes the synergistic effect of kidney tonification and anti-fatigue.

The invention discloses an environment-friendly silk fabric low-temperature plasma anti-wrinkle finishing process, and particularly relates to the technical field of biopolymer engineering. The process sequentially comprises the steps of pre-cleaning, low-temperature plasma activation, polyproline solution impregnation, roll compacting and pre-drying, acetylation treatment, neutralization and soaping, dehydration and drying and the like. Performing micro-etching and activation on the surface of the silk through oxygen low-temperature plasma to increase reaction sites; constructing a biological cross-linked network imitating the elasticity of spider silk on the surface of the fiber by utilizing polyproline with a II-type helical structure; unreacted amino groups are sealed through acetylation treatment, so that the hydrophobicity and the network stability are enhanced. The process does not need to use formaldehyde resin, keeps high breaking strength retention rate and washable stability while realizing excellent wrinkle resistance, has the comprehensive advantages of environmental protection and excellent durability, and is suitable for wrinkle-resistant function finishing of high-end silk products.

The utility model belongs to the field of agricultural machinery, and particularly relates to a bionic ditching and pressing device which comprises a ditching device and a seed furrow pressing device. The working section of a seed furrow pressing wheel in the seed furrow pressing device is a spider silk-like spindle knot curve, the equation of the spider silk-like spindle knot curve is y = 0.0397 * 2-0.9936 x + 6.3198, the value range of x is 0-25 mm, and the value range of y is 0-6.3198 mm. The device can realize the functions of ditching, seed furrow pressing and furrow shape construction, after seed furrow pressing, macropores of soil at the bottom of a seed furrow are reduced, the proportion of small pores is increased, the capillary effect of soil moisture is enhanced, and the moisture retention capacity of a seedbed is improved; a spider silk-like spindle knot curve ditch bottom formed by the bionic working section of the seed ditch press wheel has a certain water collecting effect; the formed seedbed environment is good in soil moisture content, corn seed germination and seedling emergence are facilitated, and the problem of seedbed moisture loss caused by traditional V-shaped seed ditches is solved.

The invention relates to the technical field of biomimetic materials, polymer composite materials and polymer fibers, and discloses a preparation method of biomimetic assembled high-performance artificial spider silk fibers, which comprises the following steps: preparing primary gel fibers containing rigid network nodes and flexible polymer chains; stretching the primary gel fiber in a closed environment in which the temperature and the solvent vapor pressure are controlled at the same time; according to the environment, solvent molecules preferentially swell the flexible polymer chain, the rigid network nodes are kept stable, and the applied drawing force drives the flexible polymer chain to slide and axially orientate among the rigid network nodes. According to the method, the specific stretching environment is constructed, internal physical conflicts of stretching gain and structural damage are remodeled into a synergistic process, the situation that microdefects are generated at a rigid-flexible interface by stretching force is avoided, and a bionic rigid-flexible composite structure which is difficult to achieve in the prior art and has high integrity is obtained for final fibers.

Cosmeceutical compositions comprising a major ampullate spidroin protein (MaSp)-based fiber are disclosed. Articles comprising the compositions are further disclosed.

The invention discloses a spider silk-like hybrid polymer fiber and a preparation method thereof, and belongs to the field of synthetic fibers, and the preparation method comprises the following steps: dissolving, heating and stirring a carboxyl-containing polymer until the carboxyl-containing polymer is completely swelled, and adding an acid solution for adjustment to obtain polymer gel; adding an amino-containing polymer into the polymer gel, heating and uniformly stirring to obtain hybrid gel, and centrifuging the hybrid gel to obtain a hybrid gel spinning precursor stock solution; extruding the hybrid gel spinning precursor stock solution into an anti-solvent coagulating bath, collecting fibers, and obtaining hybrid polymer fibers after moisture absorption is balanced; and carrying out pre-stretching and moisture absorption balance to obtain the spider silk-like hybrid polymer fibers with different uniaxial orientation and crystallinity structures. According to the invention, continuous preparation of the fiber is realized by combining the high-molecular polymer with anti-solvent induced phase separation and pre-stretching strain programming, the method is simple, the raw material source is environment-friendly, and the prepared fiber has comprehensive characteristics similar to spider silk.

The invention provides a preparation method of spider protein synthetic leather, which comprises the following steps: S10, preparing surface layer slurry: selecting spider protein powder with the average particle size D50 of 1.0-10.0 microns, dispersing the spider protein powder in a polar organic solvent for pre-infiltration treatment to obtain suspension liquid, and drying the suspension liquid to obtain the surface layer slurry; then mixing the suspension liquid with a polyurethane surface layer resin solution, and uniformly dispersing to prepare surface layer slurry containing spider protein powder; s20, preparing inner-layer slurry: preparing polyurethane inner-layer slurry which does not contain arachnoid protein powder; s30, dry molding: coating release paper with the surface layer slurry, drying the surface layer slurry to form a surface layer, and controlling the ratio of the dry film thickness of the surface layer to the average particle size D50 of the spider protein powder to be (5: 1)-(10: 1); and then coating the surface layer with the inner layer slurry, drying to form an inner layer, attaching base cloth to the inner layer, and curing and stripping to obtain the spider protein synthetic leather. The synthetic leather prepared by the preparation method has good mechanical properties, good breathable effect and excellent low-temperature folding resistance.

The application provides a bionic spider robot for desert tree planting and a working method thereof, and relates to the technical field of robots.The robot comprises a head mechanism, a neck mechanism, a walking mechanism and a body mechanism;the body mechanism comprises an upper bottom plate and a lower bottom plate, the upper end of the upper bottom plate is provided with a body shell, a water tank and a water pump are arranged in the body shell, solar panels are arranged on the outer side of the body shell, and the body mechanism further comprises a soil drilling mechanism, a sapling transportation mechanism, a soil cultivating mechanism and a winding mechanism.The robot in the application imitates the principle of spider silk spinning, and combines automation design and principle so that the bionic spider robot can automatically wind the planted saplings from the tail, the unique winding design can make the saplings support and depend on each other when being planted, and greatly increases the resistance to wind sand and the survival rate of planting.

The invention belongs to the field of biomedical materials and tissue engineering, and particularly relates to a composite gel scaffold based on verruca vestita spider silk protein and a preparation method of the composite gel scaffold, and the composite gel scaffold is used for repairing meniscus cartilage injury. According to the scaffold, the mechanical performance of spider silk protein, the microenvironment simulation function of chondroitin sulfate and the induced differentiation effect of BMP-2 growth factors are integrated, and in-situ regeneration of cartilage tissue is achieved.

A fusion protein and modified cellulose, as well as a preparation method, contain a protein that can bind to cellulose. This fusion protein can significantly improve the properties of cellulose. Compared to the original cellulose membrane, the fused spider silk membrane has significantly improved softness and maximum tensile strength, and its tear resistance is greatly enhanced.

The invention relates to the technical field of leather materials, and particularly discloses wear-resistant leather with an enhanced protein bionic structure and a preparation method of the wear-resistant leather. The wear-resistant leather sequentially comprises a multi-stage bionic wear-resistant surface layer, a protein bionic core layer and base cloth from top to bottom, pangolin scale macroscopic ribs and sharkskin microcosmic grooves are re-carved on the multi-stage bionic wear-resistant surface layer, and a nano hard-phase filler is compounded; the protein bionic core layer adopts a spider silk multistage fiber structure and a shell hard phase embedding mechanism, and a dynamic cross-linked network is introduced; and the transition layer and the dynamic cross-linking bonding layer realize interlayer stable combination and micro-damage self-repairing. The preparation method comprises the steps of raw material pretreatment, raw material preparation of each layer, step-by-step forming, composite curing and the like. Through multistage collaborative bionic and dynamic crosslinking design, the problems that traditional protein-based leather is poor in wear resistance and insufficient in long-term effect are solved, meanwhile, the skin-friendly and environment-friendly advantages are reserved, the wear resistance, the tearing strength and the interlayer binding force are remarkably improved, and the protein-based leather is suitable for high-end shoes, automobile interiors and other scenes.