54 results about "Spidroin" patented technology

The invention discloses a method for preparing spider silk protein/polylactic acid composite nano-fiber yarn. The method comprises the following steps of preparing a spinning solution, preparing a bath solution and then adopting an improved electrostatic spinning method to guide, heat, wind and shape bunched fibers, so as to obtain the spider silk protein/polylactic acid composite nano-fiber yarn. The composite nano-fiber yarn of the invention can be continuously spun for more than 10 hours and have no broken ends, and a certain amount of spider silk protein effectively reduces the diameter of the fibers and improves the strength of the yarn. In addition, the fracture strength and flexibility of the composite nano-fiber yarn can be further improved through secondary stretching and combined twisting, so that the composite nano-fiber yarn can be applied in biomedical materials and other fields better.

The invention relates to natural polypeptides material for biomineralization and a preparation method thereof, in particular to a natural polypeptides material for preparing a crystal structure with fine biocompatibility by mineralization, belonging to the biological medical material field. The polypeptides material containing natural fibroin consists of degumming fibroin of insect silk and spidroin, which can be used for crystals mineralized at room temperature including zinc oxide, ferric oxide, gallium oxide, cobalt oxide, titanium oxide, hydroxylapatite, calcium phosphate and calcium carbonate. The silk fibroin solution after degumming is subjected to water washing, acid hydrolysis, dialysis, centrifuge enrichment to obtain the natural polypeptides material for mineralization, natural polypeptides material can be used for preparing composite materials with fine biocompatibility by mineralization.

A method of producing polymers of an isolated spider silk protein involves providing a solution of said spider silk protein in a liquid medium at pH 6.4 or higher and/or an ion composition that prevents polymerisation of the spider silk protein. The properties of the liquid medium are adjusted to a pH of 6.3 or lower and an ion composition that allows polymerisation of the spider silk protein. The spider silk protein is allowed to form polymers in the liquid medium, and the resulting spider silk protein polymers are isolated from the liquid medium. The resulting polymers are useful as fibers, films, foams, nets or meshes.

The invention belongs to molecular biology and biomaterial fields, spider dragline silk gene is used as a base for artificial directed mutation of a part of amino acid sequence, a recombinant spider silk protein is obtained by an escherichia coli overexpression method and a Ni column purification technology, and after electrostatic spinning, a spider-silk-like protein is obtained, and the spider-silk-like protein has better consistency and elasticity than a natural spider silk protein. The spider-silk-like protein is an n-polyploid such as a tri-polyploid, hexa-polyploid or other integer-polyploid of an amino acid sequence shown in SEQ IDNO:5, wherein n is an integer equal to or greater than 1.

The invention discloses MRRP protein, a gene, a carrier, engineering bacteria and application of the MRRP protein, the gene, the carrier and the engineering bacteria to preparation of wound healing medicine. The MRRP protein is formed by sequentially connecting mussel attachment protein Ma1, elastic drosophila melanogaster protein Res1, a spider silk protein nitrogen end sequence Nt1, a spider silk protein middle-piece repetitive sequence SSR, a spider silk protein carbon end sequence Ct1, elastic drosophila melanogaster protein Res2 and mussel attachment protein Ma2. The MRRP protein integrates self-assembling performance of spider silk protein, high elasticity of the elastic drosophila melanogaster protein and high viscosity of the mussel attachment protein, comprehensive performance is excellent, lengths are uniform, the MRRP protein can be biodegraded completely, the yield is high by adopting a microorganism method for preparing, and chemical pollution is avoided; the MRRP protein has high elasticity, and can bond wounds, so that the wounds are bonded without using lines.

The invention relates to the field of weaving of textiles and discloses a water-absorbing composite silk fabric and a processing method thereof. The method includes steps: subjecting modified polyethylene glycol to mixed reaction with fibroin; spinning and twisting to obtain modified silk fibers; mixing spider silk protein liquid with glucan; spinning and twisting to obtain modified spider silk fibers; blending the obtained modified silk fibers with the modified spider silk fibers to obtain a water-absorbing composite silk gray fabric; subjecting the water-absorbing composite silk gray fabricto mixed reaction with protease solution, performing high-temperature enzyme deactivation, adding chitosan mixed liquid, filtering after stirring reaction, and performing freeze drying to obtain the water-absorbing composite silk fabric. The water-absorbing composite silk fabric prepared according to the method is excellent in water absorbing performance and great in drapability.

The invention discloses a ciprofloxacin dry powder inhaler and a preparation method thereof. The dry powder inhaler is prepared from a drug and a binary auxiliary material, wherein the drug accounts for 80 to 20 percent by mass of the dry powder inhaler, the binary auxiliary material accounts for 20 to 80 percent by mass of the dry powder inhaler, and the binary auxiliary material comprises a mucus diluting agent and biological adhesive protein; the mucus diluting agent is at least one of mannitol, ambroxol hydrochloride, aminothiopropionic acid, and bromhexine hydrochloride; and the biological adhesive protein is at least one of silk fibroin, albumin or spidroin. The dry powder inhaler prepared by the invention is good in mobility, good in aerodynamic performance, capable of targeting thedrug to lung and capable of effectively increasing the local concentration of the drug, has mucus diluting and biological mucosa adsorption characteristics, and is particularly suitable for a patientwith bronchiectasia caused by the chronic infection of the lung.

The invention relates to a nanoparticle emulsion loaded with a skin nutrition agent and a preparation method thereof. The nanoparticle emulsion is characterized by comprising the following ingredientsin percentage by weight: 0.1 percent to 50 percent of spider silk protein, 0.1 percent to 10 percent of bis[2-Hydroxyethyl] imino tris-(hydroxymethyl)-methane, 0.1 percent to 40 percent of the skin nutrition agent and 0.1 percent to 85 percent of a water and ethanol mixture, wherein the molecular weight of the spider silk protein is 80 to 100 kDa. The obtained nanoparticle emulsion loaded with the skin nutrition agent has higher biocompatibility, is milder, and has low irritation. Compared with other spider silk protein nanoemulsion, the nanoparticle emulsion has the advantages of higher encapsulation rate, better absorption permeability and higher stability.

Provided herein are methods of improving solubilization, extraction, and isolation of recombinant spider silk proteins with a salt and alcohol buffer. Provided herein are methods of solubilizing a recombinant spider silk protein from a host cell, comprising: providing a cell culture comprising a host cell, wherein the host cell expresses a recombinant spider silk protein; collecting an insoluble portion derived from the cell culture, wherein the insoluble portion comprises the recombinant spider silk protein; adding the insoluble portion of the host cell to a solution comprising a salt and an alcohol, thereby solubilizing the recombinant spider silk protein in the solution.

The invention relates to the technical field of biomedical science, and provides spider silk protein bone nails and a preparation method thereof. The preparation method of the spider silk protein bonenails comprises the following steps of (1) preparing a spider silk protein solution; and (2) injecting the spider silk protein solution into a mold, performing treatment with methanol or ethanol, andperforming drying so as to obtain the spider silk protein bone nails. The preparation method of the spider silk protein bone nails provided by the invention is simple to operate and can be in industrialized production; and the prepared spider silk protein bone nails have the advantages of being high in strength, high in tenacity, light, resistant to ultraviolet rays, biodegradable and the like, can be degraded into amino acids and polypeptide in human bodies, and do not have any toxic or harmful effects on the human bodies.