25 results about "Repetitive sequence" patented technology

The invention discloses a pleiotropic InDel molecular marker related to soybean yield, quality and agronomic traits and application, and relates to the technical field of molecular detection. Whole genome association analysis finds that a specific number of TC repetitive sequences exist in a specific region of a No.19 chromosome of soybean and are remarkably related to the characters of soybean yield, quality, plant height, leaf type and flowering time, and an InDel molecular marker is developed based on the TC repetitive sequences. By utilizing the InDel molecular marker, the genotype of a target site can be quickly and accurately detected, and soybean materials with different genetic backgrounds can be distinguished, so that soybean varieties with excellent agronomic traits such as yield, quality related traits, plant height and flowering time can be effectively screened in an auxiliary manner. According to the method, the breeding selection accuracy and efficiency are remarkably improved, and a powerful tool is provided for accelerating the soybean genetic improvement process.

This invention belongs to the field of molecular genetics and discloses a method and application for controlling fish fertility using in vivo gene editing technology. The applicant, for the first time, cloned a carp-specific U6 promoter and used this promoter to construct the U6 gene. MOVIE gRNA transgenic vectors and water The Cas9 vector allows for the establishment of an in vivo editing system. This strategy enables the heritability of sterility, a reproductive control trait, through hybridization of fertile parents to produce sterile offspring. The operation is highly efficient, simple, and environmentally friendly. Furthermore, since high-copy-rate short, scattered repetitive sequences exist in all fish species, this strategy has broad applicability across species.

The invention discloses a self-assembled nano-capture antibacterial peptide KIL4 and a preparation method and application thereof, and belongs to the technical field of biology, and the amino acid sequence is shown as SEQ ID No.1. The preparation method comprises the following steps: adopting an alpha-spiral heptapeptide repetitive sequence (abcdefg) 4 template, and selecting lysine to provide positive charges at positions b and c; isoleucine and leucine are respectively filled to a position a and a position d to provide intermolecular hydrophobic force, and lysine and glutamic acid are selected to be respectively filled to a position e and a position g to provide intermolecular electrostatic force; the position f of the center of the hydrophilic surface of the polypeptide is filled with tryptophan to optimize the hydrophobicity of the polypeptide, on the basis, the sequence is repeated for four times, and the invention further discloses application of the polypeptide in preparation of drugs for treating gram-positive bacteria or / and gram-negative bacteria infectious diseases. The antibacterial peptide disclosed by the invention has dual effects of resisting bacteria and capturing bacteria, has excellent biocompatibility, and provides an effective technical support for developing novel antibacterial drugs.

The invention provides an ethylene biosynthesis RhACO4 gene for regulating and controlling the aging of Chinese rose flowers and application of the ethylene biosynthesis RhACO4 gene, and belongs to the technical field of gene engineering. The coding nucleotide sequence of the RhACO4 gene is as shown in SEQ ID NO. 1. According to the invention, the fact that two genotypes (ProRhACO4A and ProRhACO4B) exist in an RhACO4 promoter region in 358 tetraploid Chinese rose populations is found for the first time, and the difference is that whether a section of GA dinucleotide tandem repeat sequence (GA-peak) which is 11 times is contained or not. The ProRhACO4A type carrying GA-peak is significantly related to a lower gene expression level, a lower endogenous ethylene synthesis amount and a longer vase life. The invention provides a key gene target, a molecular marker and a technical path for cultivating a new variety of rosa chinensis with a long flowering period through molecular marker-assisted selection or genetic engineering means.

The application discloses a Buster transposon system and application thereof, and the system comprises AnBT, CrBT, HvBT, LcBT and LlBT transposons and corresponding transposases, the nucleotide sequences of the AnBT, CrBT, HvBT, LcBT and LlBT transposons are shown in SEQ ID No. 1-5, and the transposases are wild type or enhanced type. The application obtains key elements such as target site repeat (TSD) and terminal inverted repeat (TIR) of the AnBT, CrBT, HvBT, LcBT and LlBT transposons and corresponding transposase sequences through molecular reconstitution, and constructs a set of gene transfer vector systems. The AnBT, CrBT, HvBT, LcBT and LlBT vector systems can effectively mediate gene transfer through cell verification, and have great application potential in transgenic animal preparation and gene therapy.

A Cas12 RNP complex comprising: a first fusion protein comprising an extracellular vesicle (EV) polypeptide and a Cas12 protein; and a circular crRNA (CRISPR RNA) comprising: a spacer sequence, a dire

The present invention refers to hyperactive variants of a transposase of the transposon system Sleeping Beauty (SB). The invention further refers to corresponding nucleic acids producing these variants, to a gene transfer system for stably introducing nucleic acid(s) into the DNA of a cell by using these hyperactive variants of a transposase of the transposon system Sleeping Beauty (SB) and to transposons used in the inventive gene transfer system, comprising a nucleic acid sequence with flanking repeats (IRs and / or RSDs). Furthermore, applications of these transposase variants, the transpsoson, or the gene transfer system are also disclosed such as gene therapy, insertional mutagenesis, gene discovery (including genome mapping), mobilization of genes, library screening, or functional analysis of genomes in vivo and in vitro. Finally, pharmaceutical compositions and kits are also encompassed.

The invention discloses a recombinant II-type collagen for promoting cartilage repair as well as a preparation method and application of the recombinant II-type collagen. The amino acid sequence of the recombinant II-type collagen is constructed by repeating the amino acid sequence as shown in SEQ ID NO: 1 for multiple times, and the number of repeating times is larger than or equal to 2 and smaller than or equal to 20. The recombinant II type collagen disclosed by the invention has good activity of promoting cartilage cell proliferation, and particularly, the recombinant II type collagen of the recombinant II type collagen is superior to full-length II type collagen, and can be used for promoting cartilage repair. The recombinant II-type collagen has 100% sequence identity with human II-type collagen, strictly follows a Gly-X-Y repetitive sequence mode, does not introduce heterologous amino acid residues, and does not have rejection and sensitization risks.

To provide a cell death-inducing agent capable of inducing cell death of cancer cells based on a new mechanism, a pharmaceutical composition for treating or preventing cancer comprising the cell death-inducing agent, and a cancer cell-detecting agent for detecting cancer cells to which the cell death-inducing agent can be applied.SOLUTION: An agent for inducing apoptosis of cancer cells expressing IFI16, ASC, and Caspase-1, the agent comprising a polynucleotide having at least one repeat unit in a repeat sequence of telomeric DNA.SELECTED DRAWING: None

The invention relates to microsatellite instability detection in cell-free DNA. Provided herein are methods for determining a microsatellite instability state of a sample. In one aspect, the method includes quantifying the number of different repetitive sequence lengths present at each of the more than one microsatellite loci according to sequence information to produce a locus score for each of the more than one microsatellite loci. The method further includes, for each of the more than one microsatellite locus, comparing the locus score for the given microsatellite locus to a locus-specific training threshold for the given microsatellite locus, and identifying the given microsatellite locus as unstable to produce a microsatellite instability score when the locus score for the given microsatellite locus exceeds a locus-specific training threshold for the given microsatellite locus, the microsatellite instability score includes a number of unstable microsatellite loci from more than one microsatellite locus.

The invention relates to the technical field of recombinant protein design and synthesis, and particularly discloses a recombinant spider silk protein and a synthesis method thereof. The recombinant spider silk protein provided by the invention is subjected to N-terminal and C-terminal structural domain replacement of different spider species and different spider silk types, intermediate functional domain repetitive sequence large fragment recombination and other optimization designs, and a series of recombinant spider silk proteins are obtained through a cell-free protein synthesis method and prokaryotic fermentation expression; compared with natural spider dragline silk protein or other existing recombinant spider silk protein, the spider silk protein has good composite mechanical performance and high temperature resistance, meanwhile, the molecular weight is small, the total expression quantity and soluble expression are remarkably improved, and a novel raw material and a design synthesis method of the recombinant spider silk protein are provided for development of high-performance biological materials.

The invention belongs to the technical field of biological detection, and particularly relates to a molecular beacon probe composition, a reagent and a kit for detecting miR-122. The composition comprises a molecular beacon and a specific hairpin probe, the nucleotide sequence of the molecular beacon is shown as SEQ ID NO.1, and the molecular beacon is modified with a fluorescence reporter group and a quenching group; the hairpin probe comprises two restriction enzyme cutting sites, two segments of repetitive sequences and a recognition site capable of being specifically combined with miR-122. According to the application, miR-122 is specifically combined with a hairpin probe to start a reaction, a dual-cycle cascade mechanism of target triggering, initiation chain generation and secondary chain displacement is constructed, and rapid quantitative analysis of miR-122 is realized in a single tube by a one-step method through high-fidelity recognition and exponential signal amplification of an initiation chain and a molecular beacon. The system has ultrahigh sensitivity, single-base resolution specificity and high accuracy, provides key technical support for noninvasive early screening of diseases such as liver cancer and the like, and has important clinical application value.

The invention relates to the technical field of genetic engineering, in particular to recombinant I-type human collagen as well as a preparation method and application thereof. The invention provides recombinant I-type human collagen. The recombinant I-type human collagen comprises an amino acid sequence as shown in SEQ ID NO.7 or an amino acid sequence having identity with the amino acid sequence as shown in SEQ ID NO.7 according to a sequence from an N terminal to a C terminal. The recombinant I-type human collagen disclosed by the invention is optimized and modified by taking natural I-type human collagen as an original gene sequence. The sequence has a triple-helix structure characterized by collagen, a (GlyXY) n repeated amino acid sequence, a high proline region and an RGD repeated sequence, and can be self-assembled to form good triple-helix high-stability collagenous fiber; meanwhile, the preparation process is simple, and high-yield collagen can be obtained at low cost.

The application discloses a kind of molecular markers for identifying mung bean resistance to bean weevil, specific primer group and application thereof, and belongs to the technical field of plant molecular markers.The nucleotide sequence of the molecular marker is shown as SEQ ID NO.1.The application clones and verifies for the first time VrRD22 The gene is the core resistance source of the bean weevil-resistant mung bean resource, and a functional molecular marker and detection system are developed accordingly.The marker development strategy (repeat sequence primer based on functional gene structure variation) of the application can provide a paradigm for the exploration and marker development of similar stress resistance genes, and has strong industrial practical value.

The present invention refers to hyperactive variants of a transposase of the transposon system Sleeping Beauty (SB). The invention further refers to corresponding nucleic acids producing these variants, to a gene transfer system for stably introducing nucleic acid(s) into the DNA of a cell by using these hyperactive variants of a transposase of the transposon system Sleeping Beauty (SB) and to transposons used in the inventive gene transfer system, comprising a nucleic acid sequence with flanking repeats (IRs and / or RSDs). Furthermore, applications of these transposase variants, the transposon, or the gene transfer system are also disclosed such as gene therapy, insertional mutagenesis, gene discovery (including genome mapping), mobilization of genes, library screening, or functional analysis of genomes in vivo and in vitro. Finally, pharmaceutical compositions and kits are also encompassed.

The present invention relates to polypeptides comprising a first amino acid sequence comprising one or more GG repeat sequences and a peptide or polypeptide of interest in form of a fusion protein that exhibits increased renaturation efficiency and optionally also improved expression. Also encompassed are nucleic acids encoding these polypeptides, host cells that comprise said nucleic acids, and methods for protein expression and renaturation using said nucleic acids, host cells and polypeptides.

The invention discloses antisense oligonucleotide and application thereof in preparation of a medicine for treating a neuronal intracellular inclusion body disease, and relates to the technical field of biological medicine. The invention provides an antisense oligonucleotide capable of specifically targeting an abnormal CGG repetitive amplification transcript of a NOTCH2NLC gene and a preparation method of the antisense oligonucleotide. The antisense oligonucleotide comprises a nucleotide sequence which is complementarily paired with a base in a 5 'UTR region containing a CGG repetitive sequence in a NOTCH2NLC gene, and aims to reduce the expression of toxic RNA and protein, so that a brand-new and effective treatment means is provided for treating the neuronal intracellular inclusion body disease; according to the invention, the abnormal repeated mRNA target of the NOTCH2NLC gene is found, and interference is carried out on the target, so that the generation of toxic RNA aggregation lesions and pathogenic inclusion bodies can be effectively reduced, and the pathological phenotype of a disease model is reversed. The antisense oligonucleotides of the present invention can be used for the treatment of neuronal intracellular inclusion body diseases.

Provided are a recombinant micromolecular collagen, an expression system thereof and a preparation method therefor, relating to the fields of synthetic biology, genetic engineering and biotechnology. Provided are multiple types of recombinant micromolecular collagen derived from type III and type XVII collagen, and successfully expressed; a micromolecular collagen expression system and a preparation method for the micromolecular collagen are established, the preparation method comprising constructing a dedicated chassis cell, and designing a micromolecular collagen tandem repeat sequence and a recombinant vector. The micromolecular collagen achieves a balance between transdermal absorption and biological activity, and the expression system or method causes expression of the micromolecular collagen to greatly increase yield; the cost and risk of foreign protein residue caused by using protease in vitro are avoided, and the time and cost of a subsequent purification process may also be reduced.

The present invention relates to the technical field of recombinant protein design and synthesis, and specifically discloses a recombinant spider silk protein and its synthesis method. The recombinant spider silk protein provided by the present invention undergoes optimized design such as replacement of the N-terminal and C-terminal domains of different spider species and different spider silk types, and large fragment recombination of the middle functional domain repeat sequences. A series of recombinant spider silk proteins are obtained through cell-free protein synthesis methods and prokaryotic fermentation expression. Compared with natural spider dragline silk proteins or other existing recombinant spider silk proteins, they generally have good composite mechanical properties and high-temperature resistance characteristics. At the same time, they have a relatively small molecular weight, significantly improving the total expression level and soluble expression, providing new raw materials and design and synthesis methods for the development of high-performance biomaterials.

The invention discloses a recombinant plasmid and application thereof, and belongs to the technical field of gene engineering. The invention constructs a recombinant plasmid which comprises a signal peptide, a receptor binding domain RBD, heptapeptide repetitive sequences HR1 and HR2 and a flexible connecting peptide. Wherein the signal peptide has the functions of guiding protein transportation and positioning, and can accurately guide related peptide fragments to proper cell positions. And the peptide fragments HR1 and HR2 can be automatically assembled into a 6-spiral bundle structure. The S1 protein plays a core role in virus infection and immune response, and correct folding of the S1 protein is a basis for ensuring functional integrity of the S1 protein. The S1 protein is prepared by the recombinant plasmid constructed by the invention, and the S1 protein can be correctly folded in cells and effectively anchored on cell membranes. Therefore, the antigen epitope of the S1 protein can be better displayed, and favorable conditions are provided for subsequent immunoreactions.

This invention relates to an AI-based early tumor screening system that couples the nucleosome localization cycle and mutation phase characteristics of cfDNA, belonging to the field of in vitro diagnostic technology. The system includes: a sample processing and nucleic acid extraction unit, which collects peripheral blood from subjects, purifies the plasma through two-stage centrifugation, extracts circulating cell-free DNA, and assesses its quality; a sequencing execution and data calibration unit, which constructs a library and performs whole-genome sequencing, removes abnormal fragments through quality control screening, removes repetitive sequences and corrects for biases after alignment with a human reference genome, and outputs standardized data; a core feature analysis and extraction unit, which identifies the nucleosome localization center, determines the orientation characteristics of DNA entanglement with histones, and integrates multimodal information to construct a core feature combination; and a model construction and risk assessment unit, which converts the features into a risk score and generates a screening report containing the risk level. This system achieves non-invasive, accurate, and efficient early tumor screening, covering a variety of common cancer types, and provides reliable support for early clinical diagnosis and treatment.

The invention relates to the technical field of electrochemical biosensors, in particular to an electrochemical biosensor for detecting domoic acid based on aptamer-regulated CRISPR (clustered regularly interspaced short palindromic repeats) activity as well as a preparation method and application of the electrochemical biosensor. The electrochemical biosensor comprises a'three-element 'detection system: 1, synthesizing a metal organic framework (UiO-66-NH2) loaded with methylene blue MB signal molecules as a signal label; 2, using rolling circle amplification (RCA) to construct a DNA nanostructure rich in repetitive sequences on the magnetic beads as a signal amplification platform; and 3, the sequence of the domoic acid aptamer is ingeniously designed into an activation switch of Cas12a. When target domoic acid exists, a Cas12a system is inactivated through competitive binding, so that a complete DNA nanostructure is reserved, a large number of signal tags are captured, and a strong electrochemical signal is generated.

The invention belongs to the technical field of agricultural biology, and discloses a molecular marker related to the iron content of tomato fruits and application of the molecular marker. The molecular marker is located in the 82086047-82086180th site region of a No.1 chromosome of a tomato genome SL2.50 ITAG2.4, and comprises a repetitive sequence in a nucleotide sequence as shown in SEQ ID NO: 1. The DNA methylation level of the molecular marker is in significant negative correlation with the iron content of tomato fruits. The invention also provides a primer pair, a kit and a method for detecting the methylation level of the molecular marker, and a method for screening tomato varieties with high iron content by using the molecular marker. The invention provides an effective technical means for early screening and molecular marker-assisted breeding of iron-rich tomato varieties.

Aspects of the disclosure relate to methods and compositions (e.g., kits) for detecting certain repeat-associated non-ATG (RAN) proteins in a subject (e.g., a subject having or suspected of having a disease associated with a CAG and / or CTG repeat expansion). In some embodiments, methods described by the disclosure comprise detecting one or more RAN proteins in a biological sample obtained from a subject by an immunoassay using one or more antibodies that target homopolymeric repeat regions of RAN proteins. In some embodiments, the disclosure relates to kits comprising one or more antibodies that target homopolymeric repeat regions of RAN proteins, and an immunoassay plate and / or reagents. In some embodiments, the disclosure provides methods of producing anti-RAN protein antibodies.