37 results about "Repetitive sequence" patented technology

The invention provides a beet genetic diversity analysis method based on whole genome re-sequencing. The method comprises the steps of PacBio HiFi library construction, computer sequencing, data information analysis, assembly quality evaluation and Hi-C auxiliary genome assembly. The invention further provides application, and the high-continuity beet reference genome is used for analyzing beet intraspecific genetic diversity and developing high-density molecular markers. According to the method, the high precision of HiFi, the super-long read length of Nanopore and Hi-C space interaction data are combined for the first time, the problems of repeated sequence and complex SV analysis are solved, and all-round breakthrough of genome continuity, annotation integrity, genetic diversity coverage and epigenetic integration is achieved.

The invention discloses a pleiotropic InDel molecular marker related to soybean yield, quality and agronomic traits and application, and relates to the technical field of molecular detection. Whole genome association analysis finds that a specific number of TC repetitive sequences exist in a specific region of a No.19 chromosome of soybean and are remarkably related to the characters of soybean yield, quality, plant height, leaf type and flowering time, and an InDel molecular marker is developed based on the TC repetitive sequences. By utilizing the InDel molecular marker, the genotype of a target site can be quickly and accurately detected, and soybean materials with different genetic backgrounds can be distinguished, so that soybean varieties with excellent agronomic traits such as yield, quality related traits, plant height and flowering time can be effectively screened in an auxiliary manner. According to the method, the breeding selection accuracy and efficiency are remarkably improved, and a powerful tool is provided for accelerating the soybean genetic improvement process.

This invention belongs to the field of molecular genetics and discloses a method and application for controlling fish fertility using in vivo gene editing technology. The applicant, for the first time, cloned a carp-specific U6 promoter and used this promoter to construct the U6 gene. MOVIE gRNA transgenic vectors and water The Cas9 vector allows for the establishment of an in vivo editing system. This strategy enables the heritability of sterility, a reproductive control trait, through hybridization of fertile parents to produce sterile offspring. The operation is highly efficient, simple, and environmentally friendly. Furthermore, since high-copy-rate short, scattered repetitive sequences exist in all fish species, this strategy has broad applicability across species.

The invention discloses a self-assembled nano-capture antibacterial peptide KIL4 and a preparation method and application thereof, and belongs to the technical field of biology, and the amino acid sequence is shown as SEQ ID No.1. The preparation method comprises the following steps: adopting an alpha-spiral heptapeptide repetitive sequence (abcdefg) 4 template, and selecting lysine to provide positive charges at positions b and c; isoleucine and leucine are respectively filled to a position a and a position d to provide intermolecular hydrophobic force, and lysine and glutamic acid are selected to be respectively filled to a position e and a position g to provide intermolecular electrostatic force; the position f of the center of the hydrophilic surface of the polypeptide is filled with tryptophan to optimize the hydrophobicity of the polypeptide, on the basis, the sequence is repeated for four times, and the invention further discloses application of the polypeptide in preparation of drugs for treating gram-positive bacteria or / and gram-negative bacteria infectious diseases. The antibacterial peptide disclosed by the invention has dual effects of resisting bacteria and capturing bacteria, has excellent biocompatibility, and provides an effective technical support for developing novel antibacterial drugs.

The invention belongs to the field of chemical synthesis, and particularly relates to a chemical synthesis method of elastin-like polypeptide. The invention provides a chemical synthesis method of elastin-like, which comprises the following steps of: constructing a pentapeptide repetitive sequence (valine-proline-glycine-X-glycine, X is amino acid except proline, and the amino acid except glycine is respectively and independently selected from levoamino acid or dextroamino acid) of elastin-like polypeptide through liquid phase reaction; the elastin-like polypeptide can be prepared with the target molecular weight of 1000-500000 by adding amino groups and carboxyl groups into an organic solvent, forming orthogonal protection on the amino groups and the carboxyl groups, then selectively removing amino or carboxyl end protecting groups respectively, accurately coupling the amino groups and the carboxyl groups in a solution and repeating the deprotection and coupling processes for multiple times. The preparation method can realize accurate regulation and control of the molecular weight, sequence, chirality and topological structure of the elastin-like protein, improves the synthesis efficiency of the high-molecular-weight elastin-like polypeptide, has the advantages of large synthesis scale, low cost and the like, and has a wide application prospect.

The invention provides an ethylene biosynthesis RhACO4 gene for regulating and controlling the aging of Chinese rose flowers and application of the ethylene biosynthesis RhACO4 gene, and belongs to the technical field of gene engineering. The coding nucleotide sequence of the RhACO4 gene is as shown in SEQ ID NO. 1. According to the invention, the fact that two genotypes (ProRhACO4A and ProRhACO4B) exist in an RhACO4 promoter region in 358 tetraploid Chinese rose populations is found for the first time, and the difference is that whether a section of GA dinucleotide tandem repeat sequence (GA-peak) which is 11 times is contained or not. The ProRhACO4A type carrying GA-peak is significantly related to a lower gene expression level, a lower endogenous ethylene synthesis amount and a longer vase life. The invention provides a key gene target, a molecular marker and a technical path for cultivating a new variety of rosa chinensis with a long flowering period through molecular marker-assisted selection or genetic engineering means.

The application discloses a Buster transposon system and application thereof, and the system comprises AnBT, CrBT, HvBT, LcBT and LlBT transposons and corresponding transposases, the nucleotide sequences of the AnBT, CrBT, HvBT, LcBT and LlBT transposons are shown in SEQ ID No. 1-5, and the transposases are wild type or enhanced type. The application obtains key elements such as target site repeat (TSD) and terminal inverted repeat (TIR) of the AnBT, CrBT, HvBT, LcBT and LlBT transposons and corresponding transposase sequences through molecular reconstitution, and constructs a set of gene transfer vector systems. The AnBT, CrBT, HvBT, LcBT and LlBT vector systems can effectively mediate gene transfer through cell verification, and have great application potential in transgenic animal preparation and gene therapy.

A Cas12 RNP complex comprising: a first fusion protein comprising an extracellular vesicle (EV) polypeptide and a Cas12 protein; and a circular crRNA (CRISPR RNA) comprising: a spacer sequence, a dire

The present invention refers to hyperactive variants of a transposase of the transposon system Sleeping Beauty (SB). The invention further refers to corresponding nucleic acids producing these variants, to a gene transfer system for stably introducing nucleic acid(s) into the DNA of a cell by using these hyperactive variants of a transposase of the transposon system Sleeping Beauty (SB) and to transposons used in the inventive gene transfer system, comprising a nucleic acid sequence with flanking repeats (IRs and / or RSDs). Furthermore, applications of these transposase variants, the transpsoson, or the gene transfer system are also disclosed such as gene therapy, insertional mutagenesis, gene discovery (including genome mapping), mobilization of genes, library screening, or functional analysis of genomes in vivo and in vitro. Finally, pharmaceutical compositions and kits are also encompassed.

The invention discloses a recombinant II-type collagen for promoting cartilage repair as well as a preparation method and application of the recombinant II-type collagen. The amino acid sequence of the recombinant II-type collagen is constructed by repeating the amino acid sequence as shown in SEQ ID NO: 1 for multiple times, and the number of repeating times is larger than or equal to 2 and smaller than or equal to 20. The recombinant II type collagen disclosed by the invention has good activity of promoting cartilage cell proliferation, and particularly, the recombinant II type collagen of the recombinant II type collagen is superior to full-length II type collagen, and can be used for promoting cartilage repair. The recombinant II-type collagen has 100% sequence identity with human II-type collagen, strictly follows a Gly-X-Y repetitive sequence mode, does not introduce heterologous amino acid residues, and does not have rejection and sensitization risks.

The present technology relates to compositions and methods for modulating expression of genes, which include a target oligonucleotide sequence, such as repeats of a particular oligonucleotide sequence containing 3 to 10 nucleotides. In particular aspects, the present technology relates to agents having a formula A-L-B, wherein -L- is a linker; A- is a Brd4 binding moiety; and —B is a nucleic acid binding moiety, such as a polyamide or complementary oligonucleotide, that specifically binds to the target oligonucleotide sequence.

To provide a cell death-inducing agent capable of inducing cell death of cancer cells based on a new mechanism, a pharmaceutical composition for treating or preventing cancer comprising the cell death-inducing agent, and a cancer cell-detecting agent for detecting cancer cells to which the cell death-inducing agent can be applied.SOLUTION: An agent for inducing apoptosis of cancer cells expressing IFI16, ASC, and Caspase-1, the agent comprising a polynucleotide having at least one repeat unit in a repeat sequence of telomeric DNA.SELECTED DRAWING: None

The present invention discloses a method for establishing a cervical disease progression prediction model based on low-depth whole-genome sequencing technology, which uses cervical scraping or vaginal swabs to collect exfoliated cells, extract DNA, and perform whole-genome sequencing to obtain the original offline data of low-depth whole-genome sequencing of the DNA sample; the above-mentioned original data is subjected to standard quality control, and then compared with the human reference genome sequence and the repetitive sequences are marked; the genome instability index is calculated by the following formula:; In addition, the present invention also relates to a method for constructing a cervical disease prediction model; the present invention is conducive to improving the accuracy and sensitivity of detection, reducing the cost of detection, and improving the accessibility of cervical disease screening and prediction, while contributing to the early diagnosis of cervical disease and the formulation of treatment decisions, and improving the management and treatment effects of patients with cervical disease.

The invention relates to a plasmid vector for detecting the translation condition of amino acid sequence protein and application of the plasmid vector, and belongs to the technical field of biology. The plasmid vector for detecting the protein translation condition of the amino acid sequence, provided by the invention, is loaded with an exogenous amino acid sequence, and the amino acid sequence contains an amino acid repetitive sequence; a fluorescent protein coding gene of one color is inserted in the upstream of an amino acid repetitive sequence coding region, and a fluorescent protein coding gene of another color is inserted in the downstream of the amino acid repetitive sequence coding region. According to the invention, the translation process of the protein containing the amino acid repetitive sequence in the cell can be monitored in real time according to the expression condition of the fluorescent protein, and the formation process of the truncated object after translation blocking is captured in real time, so that a favorable tool is provided for researching and evaluating the translation condition of the protein containing the amino acid repetitive sequence in the cell; the method can also be further applied to pathogenesis research of related diseases such as Huntington's disease and / or neuronal intracellular inclusion body disease.

The invention relates to microsatellite instability detection in cell-free DNA. Provided herein are methods for determining a microsatellite instability state of a sample. In one aspect, the method includes quantifying the number of different repetitive sequence lengths present at each of the more than one microsatellite loci according to sequence information to produce a locus score for each of the more than one microsatellite loci. The method further includes, for each of the more than one microsatellite locus, comparing the locus score for the given microsatellite locus to a locus-specific training threshold for the given microsatellite locus, and identifying the given microsatellite locus as unstable to produce a microsatellite instability score when the locus score for the given microsatellite locus exceeds a locus-specific training threshold for the given microsatellite locus, the microsatellite instability score includes a number of unstable microsatellite loci from more than one microsatellite locus.

The invention relates to pichia pastoris engineering bacteria for expressing feline-derived erythropoietin recombinant protein, and belongs to the technical field of genetic engineering bacteria. The amino acid sequence of the feline-derived erythropoietin recombinant protein is as shown in SEQ ID NO: 1. According to the invention, a yeast codon preference DNA repetitive sequence, GC content and the like of the feline-derived erythropoietin are optimized, the codon adaptation index of the feline-derived erythropoietin is increased to 0.8-1, the GC content is optimized to 40-60%, and an optimized feline-derived erythropoietin coding gene segment is obtained; the feline-derived erythropoietin recombinant protein can be effectively translated and modified in the pichia pastoris, so that the efficient expression of the feline-derived erythropoietin recombinant protein in the pichia pastoris is realized, the enzyme production efficiency of the recombinant pichia pastoris in the fermentation process is improved, the immunogenicity is reduced, and the activity and purity of the target protein are improved.

The invention discloses a primer combination for quantitatively detecting cell telomerase activity, a kit and application, and relates to the technical field of telomerase activity detection. According to the primer combination for detecting telomerase activity and the detection method provided by the invention, the primer TS and the primer ACX-UP1 with specific sequences are selected, and the primer ACX-UP1 is provided with a stem-loop structure containing a fluorophore and a quenching group, so that not only can the functions of the primer be realized, but also the functions of a probe can be realized; therefore, the problems that a probe cannot be designed on a telomere repetitive sequence and the specificity of a dye method is poor are solved. The primer combination and the detection method provided by the invention have the characteristics of reliability, stability, convenience, rapidness and accuracy, so that the manpower, material resources and time cost required by telomerase activity detection are greatly reduced, and the accuracy is greatly improved.

This invention relates to rare earth element binding protein and methods of recovering a rare earth element (REE) from a sample. The (REE) binding protein comprises the repeating sequence X1X2X3X4X5X6X7X8X9 wherein X denotes any amino acid, and X1 is D or E, X2 is A, T, or S, X3 is D, E or N, X4 is G, A, or F, X5 is D, X6 is G, S, or D, X7 is Y, L, V, F, I, E or W, X8 is A, V, I, L, F or T, X9 is D, E, or N.

The invention relates to the technical field of recombinant protein design and synthesis, and particularly discloses a recombinant spider silk protein and a synthesis method thereof. The recombinant spider silk protein provided by the invention is subjected to N-terminal and C-terminal structural domain replacement of different spider species and different spider silk types, intermediate functional domain repetitive sequence large fragment recombination and other optimization designs, and a series of recombinant spider silk proteins are obtained through a cell-free protein synthesis method and prokaryotic fermentation expression; compared with natural spider dragline silk protein or other existing recombinant spider silk protein, the spider silk protein has good composite mechanical performance and high temperature resistance, meanwhile, the molecular weight is small, the total expression quantity and soluble expression are remarkably improved, and a novel raw material and a design synthesis method of the recombinant spider silk protein are provided for development of high-performance biological materials.

The invention belongs to the technical field of biological detection, and particularly relates to a molecular beacon probe composition, a reagent and a kit for detecting miR-122. The composition comprises a molecular beacon and a specific hairpin probe, the nucleotide sequence of the molecular beacon is shown as SEQ ID NO.1, and the molecular beacon is modified with a fluorescence reporter group and a quenching group; the hairpin probe comprises two restriction enzyme cutting sites, two segments of repetitive sequences and a recognition site capable of being specifically combined with miR-122. According to the application, miR-122 is specifically combined with a hairpin probe to start a reaction, a dual-cycle cascade mechanism of target triggering, initiation chain generation and secondary chain displacement is constructed, and rapid quantitative analysis of miR-122 is realized in a single tube by a one-step method through high-fidelity recognition and exponential signal amplification of an initiation chain and a molecular beacon. The system has ultrahigh sensitivity, single-base resolution specificity and high accuracy, provides key technical support for noninvasive early screening of diseases such as liver cancer and the like, and has important clinical application value.

The invention relates to the technical field of genetic engineering, in particular to recombinant I-type human collagen as well as a preparation method and application thereof. The invention provides recombinant I-type human collagen. The recombinant I-type human collagen comprises an amino acid sequence as shown in SEQ ID NO.7 or an amino acid sequence having identity with the amino acid sequence as shown in SEQ ID NO.7 according to a sequence from an N terminal to a C terminal. The recombinant I-type human collagen disclosed by the invention is optimized and modified by taking natural I-type human collagen as an original gene sequence. The sequence has a triple-helix structure characterized by collagen, a (GlyXY) n repeated amino acid sequence, a high proline region and an RGD repeated sequence, and can be self-assembled to form good triple-helix high-stability collagenous fiber; meanwhile, the preparation process is simple, and high-yield collagen can be obtained at low cost.

The application discloses a kind of molecular markers for identifying mung bean resistance to bean weevil, specific primer group and application thereof, and belongs to the technical field of plant molecular markers.The nucleotide sequence of the molecular marker is shown as SEQ ID NO.1.The application clones and verifies for the first time VrRD22 The gene is the core resistance source of the bean weevil-resistant mung bean resource, and a functional molecular marker and detection system are developed accordingly.The marker development strategy (repeat sequence primer based on functional gene structure variation) of the application can provide a paradigm for the exploration and marker development of similar stress resistance genes, and has strong industrial practical value.

This invention relates to rare earth element binding protein and methods of recovering a rare earth element (REE) from a sample. The (REE) binding protein comprises the repeating sequence X1X2X3X4X5X6X7X8X9 wherein X denotes any amino acid, and X1 is D or E, X2 is A, T, or S, X3 is D, E or N, X4 is G, A, or F, X5 is D, X6 is G, S, or D, X7 is Y, L, V, F, I, E or W, X8 is A, V, I, L, F or T, X9 is D, E, or N.

The present invention refers to hyperactive variants of a transposase of the transposon system Sleeping Beauty (SB). The invention further refers to corresponding nucleic acids producing these variants, to a gene transfer system for stably introducing nucleic acid(s) into the DNA of a cell by using these hyperactive variants of a transposase of the transposon system Sleeping Beauty (SB) and to transposons used in the inventive gene transfer system, comprising a nucleic acid sequence with flanking repeats (IRs and / or RSDs). Furthermore, applications of these transposase variants, the transposon, or the gene transfer system are also disclosed such as gene therapy, insertional mutagenesis, gene discovery (including genome mapping), mobilization of genes, library screening, or functional analysis of genomes in vivo and in vitro. Finally, pharmaceutical compositions and kits are also encompassed.

The present invention relates to polypeptides comprising a first amino acid sequence comprising one or more GG repeat sequences and a peptide or polypeptide of interest in form of a fusion protein that exhibits increased renaturation efficiency and optionally also improved expression. Also encompassed are nucleic acids encoding these polypeptides, host cells that comprise said nucleic acids, and methods for protein expression and renaturation using said nucleic acids, host cells and polypeptides.

The invention discloses antisense oligonucleotide and application thereof in preparation of a medicine for treating a neuronal intracellular inclusion body disease, and relates to the technical field of biological medicine. The invention provides an antisense oligonucleotide capable of specifically targeting an abnormal CGG repetitive amplification transcript of a NOTCH2NLC gene and a preparation method of the antisense oligonucleotide. The antisense oligonucleotide comprises a nucleotide sequence which is complementarily paired with a base in a 5 'UTR region containing a CGG repetitive sequence in a NOTCH2NLC gene, and aims to reduce the expression of toxic RNA and protein, so that a brand-new and effective treatment means is provided for treating the neuronal intracellular inclusion body disease; according to the invention, the abnormal repeated mRNA target of the NOTCH2NLC gene is found, and interference is carried out on the target, so that the generation of toxic RNA aggregation lesions and pathogenic inclusion bodies can be effectively reduced, and the pathological phenotype of a disease model is reversed. The antisense oligonucleotides of the present invention can be used for the treatment of neuronal intracellular inclusion body diseases.

This invention discloses a molecular marker for identifying non-photosensitive eggplant and its application. The molecular marker is located at -1959bp to -1234bp of the SmMYB113 promoter in eggplant, and its nucleotide sequence is shown in SEQ ID NO:1. This invention discovers that the RU repeat sequence in the SmMYB113 promoter enables light-independent expression of SmMYB113 in non-photosensitive eggplant, thereby enhancing anthocyanin accumulation even in the absence of light, and can serve as a molecular marker for identifying non-photosensitive eggplant. Using the molecular marker of this invention, the accuracy of identification in 264 natural eggplant populations was verified to be 95.8%. This invention accurately locates the key gene regulating non-photosensitive eggplant and develops the corresponding molecular marker, which can provide technical support for marker-assisted selection breeding of eggplant and has significant application value in production.

Provided are a recombinant micromolecular collagen, an expression system thereof and a preparation method therefor, relating to the fields of synthetic biology, genetic engineering and biotechnology. Provided are multiple types of recombinant micromolecular collagen derived from type III and type XVII collagen, and successfully expressed; a micromolecular collagen expression system and a preparation method for the micromolecular collagen are established, the preparation method comprising constructing a dedicated chassis cell, and designing a micromolecular collagen tandem repeat sequence and a recombinant vector. The micromolecular collagen achieves a balance between transdermal absorption and biological activity, and the expression system or method causes expression of the micromolecular collagen to greatly increase yield; the cost and risk of foreign protein residue caused by using protease in vitro are avoided, and the time and cost of a subsequent purification process may also be reduced.