81 results about "Microsatellite" patented technology

The invention discloses SSR (simple sequence repeat) multiple PCR (polymerase chain reaction) primers for paternity test of grass carp and application of the SSR multiple PCR primers. The primers comprise 16 pairs of specific primers, and base sequences of the 16 pairs of specific primers are respectively shown as SEQ ID NO.1-32 in sequence. The invention further discloses a fish paternity test kit comprising the SSR multiple PCR primer, an SSR multiple fluorescent PCR method for grass carp paternity test and application of the SSR multiple PCR primer, the kit or the method in grass carp paternity test. According to the kit, 16 pairs of specific SSR primers, multiple fluorescent PCR and a universal amplification primer technology are combined, 16 sites can be detected at a time, universal primers can be repeatedly used, and compared with simple single-site detection, the efficiency is improved, and the cost is reduced; the microsatellite loci contained in the invention are 3-6 basic groups, the allele size interpretation is more accurate, and the accuracy of genotype data is improved.

The invention discloses a grass carp marker release effect evaluation method, which specifically comprises the following steps: dividing released grass carps into large grass carps and small grass carps, implanting microsatellite markers into abdominal cavities of the large grass carps, and fixing external markers on dorsal fins of the small grass carps; the released grass carp is subjected to fin ray cutting sampling, and the age, the weight and the shape of the grass carp are recorded; constructing a sample genetic archive; recapturing the released groups; and identifying the recaptured individuals, and counting released individuals in the recaptured grass carp. The larger the recapture rate is, the better the enhancement and release effect is, so that the contribution of the enhancement and release of the grass carps to the ecological quantity of all the grass carps in the river is estimated, and the enhancement and release effect of the grass carps is determined. The method is beneficial to recovery of the number of the grass carps in the river, the releasing effect of the grass carps can be known, the releasing method is improved in time, and the method has great popularization value.

The invention discloses SSR (Simple Sequence Repeat) multiple PCR (Polymerase Chain Reaction) primers for megalobrama amblycephala paternity test and application, the primers comprise seven pairs of specific primers, namely a primer pair MamGLB2501, a primer pair MamGLB2502, a primer pair MamGLB2503, a primer pair MamGLG2504, a primer pair MamGLG2505, a primer pair MamGLR2506 and a primer pair MamGLR2507, and the base sequences of the seven pairs of specific primers are sequentially shown as SEQ ID NO.1-14; the invention also discloses an SSR multiplex fluorescent PCR method for megalobrama amblycephala paternity test by using the primer and application of the primer or the method in megalobrama amblycephala paternity test. The microsatellite site amplified by the primer is 3-6 base repetition, the typing is accurate, and the polymorphism of the amplified site is high; the method is accurate in identification and low in price.

The embodiment of the invention discloses a preparation method of a kit applied to endometrial cancer microsatellite state detection. The preparation method comprises the following steps: taking a first preset volume of polymerase chain reaction amplification liquid; taking a polymerase chain reaction primer mixed solution with a second preset volume; and combining the weighed polymerase chain reaction amplification liquid and polymerase chain reaction primer mixed liquid into the kit applied to endometrial cancer microsatellite state detection. According to the embodiment, the accuracy and the stability of microsatellite state detection of the endometrial cancer can be improved, so that the kit consumed by repeatedly detecting the microsatellite state in the endometrial cancer is reduced.

The invention provides a microsatellite genotyping method, and belongs to the technical field of genotyping. According to the microsatellite genotyping method, a bar code primer is introduced into a microsatellite primer, and the bar code primer comprises a bar code and a universal primer. The universal primer can be used for increasing PCR products obtained by the microsatellite primer so as to supplement a target product obtained by the microsatellite primer, the problem of amplification of a low-quality DNA template is solved, the library building success rate is remarkably increased to 90-100% from 60-70%, the library building and sequencing times are remarkably reduced, the sample number of each time of library building and sequencing is increased, and the library building and sequencing efficiency is improved. The method has the remarkable advantages of low cost and high accuracy.

The invention discloses an SSR (Simple Sequence Repeat) primer composition for evaluating genetic diversity of channel catfish breeding population and an application method of the SSR primer composition, and belongs to the technical field of fish genetic breeding. The method comprises the following steps: modifying 5'ends of forward primers of the primers by using FAM fluorophores respectively, amplifying genome DNA of each breeding group by using the 10 pairs of microsatellite site primers respectively, analyzing amplification products, reading genotypes, analyzing parameters, and determining the genotype of the breeding group. The analysis result is used for evaluating the population polymorphism, the variation condition of the population genetic structure and the genetic differentiation degree among the populations. By applying the evaluation method disclosed by the invention, the population genetic diversity and genetic structure of continuous breeding generations of channel catfish can be systematically evaluated. The SSR primer and the genetic diversity evaluation method can be used for genetic improvement and new variety breeding of channel catfish.

The invention discloses a method for counting the number of wild rock sheep based on a microsatellite molecular marker. The method comprises the following steps: preprocessing a rock sheep manure sample and extracting DNA (Deoxyribonucleic Acid); performing double PCR amplification on the microsatellite marker, wherein each group of microsatellite primers comprises two pairs of specific primers; carrying out electrophoresis separation on a PCR product by using 12% polyacrylamide gel, and reading the size of an allele fragment of each microsatellite site through GeneMapper software after silver staining and developing; analyzing the acquired allele data by using software, and counting the number of different wild rock sheep individuals in the region according to an individual identification standard; the individual identification standard is as follows: if the genotypes of all 10 microsatellite loci are completely the same, or only one allele of one locus is different, the same individual is determined. The method is particularly suitable for non-invasive individual identification, genetic relationship identification and population quantity statistics of plateau wild rock sheep populations, and provides technical support for rock sheep resource protection and dynamic monitoring.

The invention relates to the technical field of computer images, and provides a spatial weak moving target detection method and system driven by time sequence image registration, and the method comprises the steps: obtaining a time sequence image short sequence of spatial weak moving target detection; inter-frame registration is carried out on the time sequence image short sequence to obtain a registration transformation matrix, and geometric correction is carried out on subsequent frames to complete space registration; double labeling is carried out based on the time sequence data after space registration is completed; and inputting the time sequence image short sequence subjected to spatial registration into a trained target detection model to obtain a detection result, and performing inter-frame registration on the time sequence image short sequence and constructing double annotations based on a motion sequence frame and coordinate points. The weak moving target can still keep feature alignment and track consistency under the conditions of cross-frame background disturbance and low signal-to-noise ratio; in combination with a cooperative structure of the encoder and the two-stage detection head, detection and high-precision positioning of weak targets such as space debris and microsatellites are realized, and the detection stability and accuracy of the weak and small moving targets in the space are improved.

The invention provides a cold air thruster of a microsatellite. The cold air thruster is integrated with a valve assembly, a piezoelectric actuator, an elastic mechanism, a capacitance displacement sensor, a flow sensor and a control unit. The valve assembly is driven by the executing mechanism, the gap between the disc-shaped diaphragm and the valve port is accurately regulated and controlled, and fine adjustment of gas flow and thrust is achieved. And the piezoelectric ceramic and elastic design of the actuating mechanism ensures that the valve is normally opened and quickly responded. The sensor system optimizes the control accuracy through feedback of a capacitance sensor and a flow sensor. The double-closed-loop control strategy adjusts driving voltage based on displacement and flow information, improves dynamic response and reduces control noise. The shell assembly provides comprehensive protection. The problems that in the prior art, thrust control precision is low, dynamic response performance is poor, and control noise is large can be effectively solved.

This invention discloses a method for evaluating the effectiveness of stock enhancement and release based on microsatellite markers. The method involves collecting a dataset of recaptured samples of released species; obtaining microsatellite genetic data of released broodstock, released fry, and recaptured samples; performing consistency processing on the microsatellite genetic data of released broodstock to supplement the virtual parent microsatellite genotype database; generating an allele contribution probability weight matrix to obtain an allele contribution probability dataset of recaptured samples; generating a basic matching list to construct a contribution weight dataset; and outputting the stock enhancement and release contribution rate evaluation results and storing them in a stock enhancement and release evaluation database. This invention makes the stock enhancement contribution rate no longer simply dependent on the number of matches, but comprehensively reflects the true contribution of recaptured individuals in the overall genetic background, thus improving the accuracy and robustness of stock enhancement and release effectiveness evaluation from an algorithmic perspective.

This invention belongs to the field of animal genetics and breeding technology, specifically disclosing an SNP chip for distinguishing five native Hubei yellow cattle breeds (Yiling Yellow Cattle, Dabie Mountain Yellow Cattle, Yunba Yellow Cattle, Enshi Yellow Cattle, and Sanjiaoshan Yellow Cattle). This invention combines whole-genome sequencing and three selection signals (Tajima's D, FST, and π ratio) to obtain candidate SNP loci. Random forest scoring is then applied to these candidate loci to further reduce the number of required SNP loci, ultimately obtaining breed-specific SNP loci combinations. Probes are designed based on these SNP loci and their preceding and following 60 bp sequences, enabling the development of an SNP chip for distinguishing the five native Hubei yellow cattle breeds. This provides technical support for breed selection of native Hubei yellow cattle and, compared to traditional pedigree and microsatellite identification methods, offers advantages in high accuracy and ease of operation.

This invention discloses a deployable ultra-wideband, high-gain, wide-beam circularly polarized antenna. The antenna includes a top-mounted section of the radiating arm, an integrated feed balun, a middle deployable / retractable section of the radiating arm, a bottom-mounted section of the radiating arm, a top mounting base and connector, a built-in deployable mechanism, and a metal base plate. The bottom-mounted section of the radiating arm is fixedly mounted on the metal base plate, and the middle deployable / retractable section is connected between the top-mounted and bottom-mounted sections of the radiating arm. The integrated feed balun is embedded inside the top-mounted section of the radiating arm, and the built-in deployable mechanism is fixedly mounted on the metal base plate, connecting to the integrated feed balun within the middle deployable / retractable section of the radiating arm via the top mounting base and connector. This invention achieves the deployment and retraction of the integrated antenna through the built-in deployable mechanism, offering advantages such as small size, light weight, high retraction ratio, high reliability, and ultra-wideband capability. It is suitable for the multi-functional needs of microsatellites for wireless reconnaissance, communication, and signal acquisition.

A satellite, particularly a microsatellite, comprises a plurality of compartments selected from an avionics compartment (2a), a propulsion compartment, a payload compartment. Each compartment of the plurality of compartments (2) comprises at least one module formed by a plurality of bays (6) arranged laterally with respect to one another. Each bay (6) comprises a structure (8) having at least a base wall (8A), a back wall (8B) and possibly two side walls (8C) extending laterally from the back wall, particularly with opposite inclinations, a front panel (8D) being removably connectable to the structure (8). Each bay (6) further comprises at least one onboard unit (10), a plurality of mechanical connection elements, configured to allow mutual fastening of different bays (6) arranged adjacent to each other, and at least one electrical connection element for transmission of power and / or signal. The at least one electrical connection element is configured to establish power and / or signal connection with at least one different bay (6), so as to guarantee a power and / or signal communication between onboard units (10) belonging to different bays (6).

The invention discloses a method for identifying DNA (deoxyribonucleic acid) fragments of male parent nibea albiflora in a gynogenesis pseudosciaena crocea genome. According to the scheme, the pseudosciaena crocea genome is used as a reference coordinate system; male parent positive sites in a gynogenesis pseudosciaena crocea genome are screened by identifying the specific variation sites of the nibea albiflora and analyzing the genotypes of the gynogenesis pseudosciaena crocea at the specific variation sites of the nibea albiflora; performing multi-sequence comparison, calculating the number of positive sites in comparison blocks and the proportion, filtering candidate segments and the like to establish the method for identifying the genetic material from the male parent nibea albiflora in the gynogenetic large yellow croaker genome. At present, related identification is mainly carried out through comparative analysis and microsatellite marking; the methods have the problems of high cost, low positive rate and the like; according to the scheme, genetic materials derived from male parent nibea albiflora in a gynogenetic pseudosciaena crocea genome are comprehensively and accurately screened on the whole genome level, the economic character source of gynogenetic progeny can be defined, and application such as variety identity identification and variety right protection is facilitated by developing specific molecular markers.

The application provides a method for determining salmon run time based on genetic distance and principal coordinate analysis, and belongs to the technical field of molecular biology. On the basis of obtaining salmon microsatellite data, the application makes accurate determination of salmon run time at the molecular level by using a genetic differentiation coefficient in combination with a technical means of principal coordinate component analysis. Based on the application, technical support is provided for accurate determination of the run time of anadromous fish, and the application has important significance for the study of anadromous fish.

The application discloses a coaxial pulse plasma thruster with a magnet as an outer electrode, and belongs to the technical field of microsatellite aerospace propulsion. The coaxial pulse plasma thruster with a magnet as an outer electrode mainly comprises an anode, a magnet cathode, a metal cathode, a propellant, an insulating outer shell, an insulating base, a supply spring and a spark plug. The metal cathode is divided into a perforated metal cathode and a non-perforated metal cathode. The thruster is coaxial in shape; the inner electrode is a solid cylinder, the outer electrode is a hollow pipe with a circular cross section, the solid propellant is located between the inner and outer electrodes, the supply spring is arranged between the tail of the propellant and the insulating base, and the outer electrode adopts segmented magnets and metal as the cathode. The application utilizes the magnetic field to restrict the radial movement of plasma so as to improve the collision ionization level, and provides Lorentz force to better accelerate the plasma. The segmented magnetic field provided by the segmented magnets with different magnetic poles opposite to each other can provide the magnetic field while reducing the overall mass of the thruster, and is beneficial to adjusting the magnetic field distribution in the thruster pipeline.

The invention provides a combinable general high-rigidity mechanical test tool for microsatellites, and the tool comprises a mounting plate which is horizontally or vertically arranged, is provided with regularly arranged mounting holes, and is used for mounting a bottom plate, an inclined support and an adapter when the mounting plate is vertically arranged; the bottom plate is horizontally mounted at the bottom of the mounting plate and is used for being mounted on a vibration table; the inclined support is mounted and supported between the mounting plate and the bottom plate; and the multiple adapters are correspondingly arranged on the side, away from the inclined support, of the mounting plate according to the hole distance of fixing holes of a target satellite to be measured, each adapter comprises a pressing plate and an adapter block, and the pressing plate is provided with a replacement hole, can be used for adaptively mounting the adapter blocks with mounting interfaces of different specifications, and is used for adaptively mounting different satellites. The technical problems that due to the design limitation of one type and one installation of an existing test tool, the tool cost is high, storage is difficult, management is tedious, the repeated design efficiency is low, and the requirements of multiple types of microsatellites are difficult to meet are solved.

The application discloses a kind of Megalobrama amblycephala parentage identification SSR multiplex PCR primer and application, the primer includes 7 pairs of specific primers, respectively primer pair MamGLB2501, MamGLB2502, MamGLB2503, MamGLG2504, MamGLG2505, MamGLR2506 and MamGLR2507, the base sequence of 7 pairs of specific primers is sequentially shown as SEQ ID NO.1~14;Also disclosed is the SSR multiplex fluorescence PCR method for Megalobrama amblycephala parentage identification using the above primer, and the application of the above primer or method in Megalobrama amblycephala parentage identification.The microsatellite site amplified by the primer of the application is 3-6 base repeats, accurate in typing, and high in amplification site polymorphism;The method of the application is accurate in identification and low in price.

Disclosed are compounds of formula (I) useful as Werner helicase (WRN) inhibitors, pharmaceutical compositions comprising the same, and their use for the prevention or treatment of cancers characterized by microsatellite height instability (MSI-H) or mismatch repair defects (dMMR).

The present disclosure relates to thiazolopyridine-7 (4h)-one-based compounds, including compounds of Formula I, i.e., the isomer 4H-[l, 3] thiazolo [4, 5-b] pyridine-7-one or 4H-[l, 3] thiazolo [5, 4-b] pyridine-7-one compounds, and pharmaceutically acceptable salts thereof, and compositions thereof, in particular to pharmaceutical compositions comprising the compounds of Formula I, i.e., the isomer 4H-[l, 3] thiazolo [4, 5-b] pyridine-7-one compounds. And methods of treating cancer, such as cancers involving WRN proteins, in particular cancers characterized by high microsatellite instability (MSI-H) or lack of mismatch repair (dMMR). (I)

The application discloses a multiplex PCR primer for SSR of Sparus macrocephalus and application thereof, the multiplex PCR primer for SSR of Sparus macrocephalus comprises eight pairs of specific primers, namely primer pairs XD1, XD2, XD3, XD4, XD5, XD6, XD7 and XD8, and base sequences of the eight pairs of specific primers are shown in SEQ ID NO. 1-16 in sequence respectively; a multiplex fluorescence PCR method for SSR of Sparus macrocephalus and application of the multiplex PCR primer, a kit containing the primer or a method in evaluation of genetic diversity of Sparus macrocephalus are also disclosed; the primer amplification in the application is 3-6 base repeats of SSR sites, is stable and accurate in typing, and the method comprises a group of eight microsatellite sites, is high in accuracy, low in cost and convenient to operate.

The application discloses a method for identifying the genetic relationship and ploidy of hybrid kelp polyploids, which utilizes screened SSR markers to perform PCR amplification on the parents of a hybrid combination and the hybrid kelp polyploid offspring cultivated, and performs electrophoresis detection, so as to identify the genetic relationship of the hybrid kelp polyploids and realize the identification of the ploidy of the hybrid kelp polyploids. The screened kelp SSR markers need to meet the requirement that bands capable of distinguishing the parents of the hybrid combination exist after performing PCR amplification on the DNA of the parents of the hybrid combination and performing electrophoresis detection. The application is based on the molecular biology technology of kelp microsatellite molecular markers (SSR), and simultaneously identifies the genetic relationship of the hybrid combination, and realizes the purpose of accurately identifying the ploidy of the hybrid kelp polyploids from the molecular level.

The invention belongs to the technical field of identification of variety resources and germplasm innovation, and discloses an SSR (simple sequence repeat) core primer combination for identifying the content of'high saikoside 'in a radix bupleuri variety, a kit and application. According to the invention, a pair of specific SSR core primers (CHSSR1F / CHSSR1R) are screened by using a microsatellite molecular marker development technology. The primer combination can specifically amplify a characteristic band of 136 bp in a Chinese thorowax root variety with high saikosaponin, so that the Chinese thorowax root variety with low saikosaponin can be quickly and accurately identified and distinguished from other Chinese thorowax root varieties with low saikosaponin. The SSR core primer combination, the kit and the identification method provided by the invention overcome the defects that the traditional chromatographic identification method is time-consuming, high in cost, dependent on equipment and the like, have the advantages of reliable result, simplicity and convenience in operation, high detection efficiency and the like, and are beneficial to popularization and protection of a'high saikosaponin 'radix bupleuri variety and medicinal material quality control.

The application discloses a method for constructing a marker microsatellite site stability prediction model, and belongs to the technical field of MSI detection. The application establishes a regression model through known microsatellite sites to predict the stability of 22 marker microsatellite sites. The stability data of the marker microsatellite sites is obtained by using the method, and a machine learning model can be further established to predict the MSI overall state. By using the application, the MSI overall state can be predicted without pairing normal samples in different NGS detection platforms and different cancer types through analysis of second-generation sequencing data, and the method is stable, fast, accurate, and highly repeatable, and the detection limitation is reduced.

The application relates to the technical field of satellites, in particular to a micro-satellite release device which comprises a shell, a micro-satellite, a rotating piece, a release device, the micro-satellite is located in the inside of a storage groove of the shell, one side of the storage groove is provided with a push plate one, the push plate one is connected in the inside of the storage groove through an elastic rod one, the inside of the shell is provided with a limiting groove one, the inside of the shell is provided with an elastic buckle one, the inside of the shell is provided with a waste groove, one side of the release device is provided with a detection piece, the detection piece is connected with a detection head through a detection rod, the lower portion of the detection head is provided with a push plate, one side of the detection head is provided with a connecting head, the micro-satellite can be released in two opposite directions at the same time, the angle adjustment of the device when the micro-satellite is released in the opposite direction can be avoided, and the micro-satellite can be efficiently released.

This application belongs to the field of bioassay technology, and particularly relates to microsatellite markers and their applications for brown-spotted grouper, freshwater grouper, and their hybrid offspring, the 'Shanhu' hybrid grouper. This application provides SSR molecular markers for brown-spotted grouper, freshwater grouper, and their hybrid offspring, the 'Shanhu' hybrid grouper, including the following molecular markers: ad30, ae26, ab42, ad19, ad25, ae27, and ac34; the nucleotide sequences of the molecular markers are shown in SEQ ID NO.1 to SEQ ID NO.7, and the primer pairs for amplifying these molecular markers include the nucleotide sequences shown in SEQ ID NO.8 to SEQ ID NO.21. This application provides microsatellite markers for brown-spotted grouper, freshwater grouper, and their hybrid offspring, the 'Shanhu' hybrid grouper, and their applications, providing seven SSR molecular marker combinations that can be used for population genetic diversity analysis and identification of the three grouper species.

A primer for detection of a mutation in a microsatellite contained in a target sequence of a double stranded DNA molecule, wherein the mutation is a frameshift of the microsatellite as compared with the corresponding wild type microsatellite sequence. The primer comprises a region of at least 10 nucleotides that is complementary to the target sequence of the antisense or the sense strand of the DNA molecule containing the microsatellite having a frameshift mutation, except that the primer includes between one and four, or one and three, nucleotides which are mismatched to the target sequence containing the mutation in the microsatellite and which are also mismatched to a corresponding sequence containing the wild type microsatellite.

The invention relates to the technical field of aerospace crafts, in particular to a chemical-electric hybrid mode solid propulsion device for microsatellites, which comprises a combustion chamber shell, a control electrode unit, an electric control propellant, an inner heat protection layer, a spray pipe inner heat insulation layer and an electric propeller, the control electrode unit comprises an outer electrode, the outer electrode is concentrically arranged in the combustion chamber shell, a plurality of grain holes in different reference circles are evenly distributed in the outer electrode along the center of the outer electrode, a plurality of grain holes are evenly distributed in each reference circle, and each grain hole is filled with an electric control propellant to form a grain. Inner electrodes which are concentrically arranged are embedded into the grain, and the polarities of the inner electrodes on the adjacent reference circles are opposite; wherein the head of the inner electrode is in contact with the electric control propellant, and an insulating paint layer is arranged on the outer wall of the inner electrode. According to the invention, not only can high-thrust ignition work be realized, but also a high-frequency low-thrust ignition working mode can be realized, and the requirements of multiple ignition and intelligent control of a propeller under the in-orbit service condition of a microsatellite are met.

The invention belongs to the technical field of biology, and particularly relates to a microsatellite-based primer combination for paternity test of holothuria leucospilota and application of the microsatellite-based primer combination. The sequence information of the primer combination is as shown in SEQ ID NO. 1 to SEQ ID NO. 16. According to the method, the parent-child relationship of the holothuria leucospilota is identified through genetic typing data of eight pairs of microsatellite fluorescent primers for the first time, and the method has high efficiency and accuracy. The method has high application value in the aspect of effect evaluation of enhancement and release of the holothuria leucospilota, solves the problem that a traditional physical marker is not suitable for marking juvenile holothuria leucospilota, and can relatively accurately judge whether the recaptured holothuria leucospilota is a released individual or not.

The application discloses a coil excitation type hollow cathode thruster, which comprises a bottom plate and a shell arranged at the edge of the bottom plate, and the bottom plate and the shell form a containing space. The coil excitation type hollow cathode thruster further comprises a hollow cathode structure, an anode structure and an excitation structure. The hollow cathode structure comprises a hollow cathode tube, an emitter, a heating wire and a holding electrode, the hollow cathode tube is arranged in the containing space, the emitter is arranged in the hollow cathode tube, and the heating wire is arranged on the outer wall of the hollow cathode tube and surrounds the emitter. The anode structure comprises an insulating channel and an anode, the insulating channel is arranged outside the holding electrode, and the anode is arranged in the insulating channel. The excitation structure comprises an excitation coil, a magnetic conducting column and a permanent magnet. The excitation coil excitation type electric propulsion hollow cathode thruster structure of the application is relatively simple and has low power consumption, and meets the requirements of microsatellites on microthrusters.