811 results about "Microsatellite" patented technology

PROBLEM

There are provided induced malignant stem cells capable of in vitro proliferation that are useful in cancer research and drug discovery for cancer therapy, as well as processes for production thereof, cancer cells derived from these cells, and applications of these cells.

MEANS FOR SOLVING

An induced malignant stem cell capable of in vitro proliferation are characterized by satisfying the following two requirements:

• (1) having at least one aberration selected from among (a) an aberration of methylation (high or low degree of methylation) in a tumor suppressor gene or a cancer-related genetic region in endogenous genomic DNA, (b) a somatic mutation of a tumor suppressor gene or a somatic mutation of an endogenous cancer-related gene in endogenous genomic DNA, (c) abnormal expression (increased or reduced/lost expression) of an endogenous oncogene or an endogenous tumor suppressor gene, (d) abnormal expression (increased or reduced/lost expression) of a noncoding RNA such as an endogenous cancer-related microRNA, (e) abnormal expression of an endogenous cancer-related protein, (f) an aberration of endogenous cancer-related metabolism (hypermetabolism or hypometabolism), (g) an aberration of endogenous cancer-related sugar chain, (h) an aberration of copy number variations in endogenous genomic DNA, and (i) instability of microsatellites in endogenous genomic DNA in an induced malignant stem cell; and
• (2) expressing genes including POU5F1 gene, NANOG gene, SOX2 gene, and ZFP42 gene.

The invention discloses a child-mother satellite space debris clearing platform. The child-mother satellite space debris clearing platform includes a plurality of child satellites for clearing space debris; each child satellite is detachably connected the same mother satellite; the mother satellite is used for driving the child satellites to move among a multiple pieces of space debris; a space debris clearing device is arranged on each child satellite; and each space debris clearing device includes a child measurement device for measuring a pose of the space debris and a child satellite capturing device. A microsatellite is used as the mother satellite, and a plurality of micro-nano satellites are used as the child satellites; operations such as orbit transferring, closing and detailed survey, and capturing and de-orbiting can be completed coordinately; the child-mother satellite space debris clearing platform is low in cost, is fast in response speed, and is high in reliability; after the child satellites are released, the combined space debris observation network can acquire accurate debris pose information through coordinate observation; and the child satellites can independently complete de-orbiting operations, the mother satellite can directly go to next space debris to be cleared, time and energy can be saved, the benefits of space debris clearing can be increased, and the cost is reduced.

The application relates to a method of a predicting the presence of invasive pancreatic cancer or high grade dysplasia, pre-cancerous pancreatic states and non-neoplastic conditions comprising detailed molecular analysis incorporating DNA quality and quantity, K-ras mutational analysis and a broad spectrum of tumor suppressor gene linked microsatellite LOH. Methods of diagnosing, determining prognosis of and determining a course of treatment for pancreatic cancer or high grade dysplasia, pre-cancerous pancreatic states and non-neoplastic conditions are also provided.

Micro satellite is disclosed with foldable solar panels that may be winded around the body of the micro satellite so that the growth in outer dimensions of the satellite is no more than 10-20 mm along each one of the length, width and height of the microsatellite so that the micro satellite may be launched in an auxiliary payload volume of a launcher. The foldable solar panels may be deployed to employ area that exceeds 9 times the product of the length by the width of the satellite and 6 times the product of the height by the length. The solar power produced by the solar panel and their light weight enable carrying of cargo that is at least 0.6 of the of the total mass of the satellites.

The invention discloses a method for identifying camellia oleifera cultivars and a special primer and a kit thereof; according to the invention, DNA of a camellia oleifera sample to be identified and DNA of a standard control sample are used as templates; PCR amplification is performed with the leading of a same primer pair to obtain a product; the product is treated by electrophoresis; genotype comparison of the electrophoresis results is performed; if the genotypes are different, the cultivars are different; otherwise similarity probability calculation is performed; if the detection requirements are not met, a primer is selected again for PCR identification till the identification probability requirements are met; compared with present camellia oleifera identification methods, the invention comprises the following outstanding advantages: 10 pairs of special microsatellite primers for camellia oleiferaare used to identify the genetic authenticity of camellia oleifera cultivars; the identification method has simple and rapid operations, and the identification accuracy is high. In addition, a corresponding detection kit developed by the method of the invention can be used to identify the genetic authenticity of camellia oleifera cultivars, and has the advantages of simple application method, speediness, and sensitivity; the detection results are reliable, stable, and accurate; and the kit provides reliable technical means for the identification of genetic authenticity of camellia oleifera cultivars. The invention plays an important role in the supervision and examination of the application of camellia oleifera improved varieties, has wide application prospects, has very good practicality, and can provide good economic benefit and social benefit.

The invention belongs to molecular biology DNA labeling technique and application field, concretely relates to a porphyra yezoensis microsatellite marker screening method, and a method for analyzing germplasm genetic diversity by the makers, thereby lay foundations for genetic structure analysis of porphyra yezoensis, germplasm resource protection and molecular marker auxiliary breeding.

The invention provides a method and a system for satellite telemetry speed increasing and load data fusion. A measurement and control function and a data transmission function of a microsatellite are integrally designed, and the measurement and control function and the data transmission function are realized in a measurement and control mode and a data transmission mode respectively; in the measurement and control mode, a USB (universal serial bus) measurement and control system is adopted to download telemetry data; in the data transmission mode, acquired effective load data and the telemetry data are packaged, mixed and downloaded, and then the load data and the telemetry data are analyzed on the ground through a virtual channel identification in a classified manner. According to the method and the system for satellite telemetry speed increasing and load data fusion, measurement and control data and data transmission data are effectively fused, the ratio of the measurement and control data to the data transmission data can be flexibly adjusted, and the limit rate of measurement and control data transmission is greatly increased; redundancy of on-board equipment is effectively reduced, allocation of on-board resources is reasonably optimized, the utilization rate of space is remarkably increased, power consumption is remarkably reduced, and besides, the current condition of shortage of frequency resources is relieved.

The invention relates to a microsatellite colorectal cancer instability amplification system and a detection kit thereof. The amplification system disclosed by the invention comprises a general-specific chimeric primer pair and a mixture of general primers, wherein the general-specific chimeric primer pair respectively aims at microsatellite sites BAT25, BAT26, D2S123, D5S346 and D17S250; the general primer is FluD5-Up; respective positive primer 5' ends in the general-specific chimeric primer pair are provided with a sequence of the FluD5-Up; the FluD5-Up is a fluorescent-labeled primer. The amplification system and the detection kit thereof which are disclosed by the invention overcome the shortcoming that different primers require for multiple fluorescent labels; five microsatellite gene sites are detected in the same PCR (polymerase chain reaction) system, so that a result is intuitive and reliable; the time can be saved, and the efficiency is high; MSI (medium-scale integration) gene sites can be effectively detected and analyzed.

The invention provides a modular low-impact linkage type microsatellite separating unit which comprises a launching guide module, a locking module, a pre-tightening module and a release module which are installed on a bottom plate. One end of the pre-tightening module is connected with the locking module through a pressing rod, the other end of the pre-tightening module is connected with the release module through a pull rope, and a linkage mechanism is formed. The separating unit is composed of a plurality of modular units and is simple and compact in structure, and all degrees of freedom of a fixed object are completely limited. In addition, the linkage mechanism is formed by the locking module, the pre-tightening module and the release module, and motion transfer is smooth and convenient. A grooving bolt is expanded to be broken through the release module based on shape memory alloy, so that impacts on microsatellites in the release process are extremely small, and no influence is generated on the posture and stability control over a spacecraft body and separated objects.

The invention discloses a composition for detecting the instability of a microsatellite and application of the composition. The composition comprises primer pairs respectively used for detecting specific target genes relevant with the instability of the microsatellite in a to-be-detected sample. A kit for detecting the instability of the microsatellite is used for detecting microsatellite distribution conditions of nine loci of Bat 26, Bat 25, NR-21, NR-24, NR-27, Mono-27, D5S346, D2S123 and D17S250 and three loci of Penta C, Penta D and Amel by virtue of a specific multi-fluorescence PCR fragment through an analysis capillary electrophoresis method and detecting whether the microsatellite is in a stable or instable state. By adjusting the proportion of 12 pairs of primers, the use amounts of Taq enzyme and magnesium ions, a PCR system and the like, and the sensitivity and specificity of the whole kit are improved.

The invention discloses a pinhole array miniature electrostatic electric thruster. The pinhole array miniature electrostatic electric thruster comprises a neutralizer and a thruster body which are mutually connected, wherein the thruster body sequentially comprises a grid component, a pinhole array component and a propellant storage chamber which are cooperatively connected from top to bottom; the pinhole array component is formed by conveying plates and a pinhole array plate; the conveying plates are arranged on the lower surface of the pinhole array plate; pinhole arrays are uniformly distributed in the pinhole array plate; the grid component consists of an extraction grid, an acceleration grid and an insulation bracket; the extraction grid, the acceleration grid and the pinhole array plate are sequentially connected through the insulation bracket; and coaxial pinholes corresponding to the pinhole arrays are formed in the extraction grid and the acceleration grid; and heaters and the propellant are all located in the propellant storage chamber. The pinhole array miniature electrostatic electric thruster has the advantages that the structure is simple; the size is small; the specific impulse can reach 700-2000 s; the propellant storage function and the conveying function are integrated; the propellant is stored in a pressure-free mode; no movable components exist; and the like. The pinhole array miniature electrostatic electric thruster can be packaged in a chip mode, and can be applied to microsatellites as a standard thrusting module.

The invention provides a DNA probe library for hybridization with MSI (micro-satellite instability) detection related micro-satellite sites. The DNA probe library comprises one or more DNA probes capable of hybridizing with the MSI state related microsatellite sites. In the DNA probes, the MSI related micro-satellite sites are shown in the following sequences: SEQ ID NO. 1-66. Besides, the invention provides a method for enriching and detecting the MSI related micro-satellite sites by the probe library. With combination of the method with the next generation sequencing technology (NGS), sensitivity, accuracy and comprehensiveness of MSI detection can be greatly improved.

The invention relates to a method which uses microsatellite marking technology for identifying the animal origin ingredients in meat products, belonging to the biological high-tech field. The use of the microsatellite marking technology for identifying the origin ingredients of cattle, sheep, pigs, dogs, chickens and ducks in the meat products comprises the extraction of genomic DNA in meat products, the conducting of PCR reaction, the PCR product detection and primer screening, and the identification of samples. The method selects microsatellite marks with specific property among the types and universal property in the type, designs specific primers, establishes PCR detection methods, and identifies the origin ingredients of cattle, sheep, pigs, dogs, chickens and ducks in the meat products according to the size and the availability of amplification fragment. The invention has the advantages of rapidity, simplicity, accuracy, strong specificity and high sensitiveness. The invention is suitable for being promoted and used for detecting multitudinous samples. The invention also has great significance for preventing the mad cow disease from being spread into China and the illegal businessmen from concealing the commodity elements, and ensuring national security and the health of people.

The invention discloses an FPGA (Field Programmable Gate Array) key data protection method for a microsatellite, and aims to solve the technical problem of poor protection capability due to error accumulation in an existing FPGA data protection method. According to the technical scheme, FPGA resources are partitioned into a local structure, memory resources and an AHB-Lite bus firstly. The memory resources of key data are partitioned into three key memories and one ordinary memory in a way of mounting a slave memory on the AHB-Lite bus. Single-bit data detection and correction and multi-bit data detection are realized in combination with a three-mode redundancy technology and an error correction detection technology. If a single-bit error occurs, data is corrected through an error correction detection function. If a multi-bit error occurs, detection interrupt and a corresponding error data address are generated by the error correction detection function; the memories are re-read with the three-mode redundancy technology; and memory error data is corrected. If single particle upset is detected, the memories are written back, so that device memory data returns to normal.

The invention belongs to the technical field of indoor positioning, and discloses a microsatellite base station positioning system and a method based on a single or multiple Bluetooth transmitting units. The positioning system comprises at least one microsatellite base station for broadcasting the identification code, coordinates, the flow and the room number of the base station, transmitter signal propagation parameters and working mode message information. A microsatellite base station positioning system on user equipment is used for receiving the signals of the microsatellite base station, analyzing the obtained message information and calculating self one-dimensional, two-dimensional or three-dimensional coordinates. Precision time synchronization and dedicated receiving equipment are not needed, user habits are not changed, and a general phone can be used to acquire the precision equivalent to that of outdoor positioning; seamless butt joint between indoor positioning and outdoor positioning can be realized; and the microsatellite base station is low in cost, low in power consumption, convenient to deploy and good in environmental adaptability and extendibility, and no external cable and no fiber is needed.

The invention discloses a coaxial anode insulating pulse plasma thruster and relates to the technical field of microsatellite thrusters. The coaxial anode insulating pulse plasma thruster comprises acathode, an anode and an insulating barrel sleeving the cathode, wherein the cathode is cylindrical, a discharge end is arranged at the front end of the cathode, the anode is of a cylindrical structure fixed to the outer wall of the insulating barrel, and the inner wall of the anode is in contact with the outer wall of the insulating barrel; an insulating layer wraps the outer surface of the anode; axes of the cathode, the anode and the insulating barrel are superposed; the cylindrical end of the cathode is electrically connected to a negative high voltage terminal of an external circuit; andthe anode is grounded. The anode is wrapped by means of the insulating barrel and the insulating layer, so that a passage where plasmas move toward the anode is blocked, and more plasmas are ejected axially along the insulating barrel, and therefore, the density of a plasma jet is improved. By arranging the insulating barrel, the plasmas under the action of hump potential are ejected axially alongthe insulating barrel to form the plasma jet which is high in energy and good in directionality.

A multi-code-rate multi-channel multi-system measurement and control system of a microsatellite comprises a transmit-receive antenna, a USB responder, a spread spectrum responder, an antenna integration USB responder, an antenna integration spread spectrum responder, a microwave network and a comprehensive electronic computer. The comprehensive electronic computer is respectively in communication connection with the USB responder, the spread spectrum responder, the antenna integration USB responder and the antenna integration spread spectrum responder, the USB responder and the spread spectrum responder are in communication connection with the transmit-receive antenna through the microwave network, and the antenna integration USB responder and the antenna integration spread spectrum responder each comprises an integrated measurement and control antenna. The multi-code-rate multi-channel multi-system measurement and control system has the advantages that research cost of a spacecraft is reduced, overall reliability of the tiny spacecraft is improved, the antenna and the responder are integrated, the weight is low and power consumption is low.

The invention discloses an advanced electronic integrated system for a microsatellite. The system comprises a universal computer, a power control module, a USB (universal serial bus) or spread-spectrum measurement and control module, a GNC (global navigation chart) module, a driving control module and a data transmission module, wherein the universal computer is used for the unified management and processing of the microsatellite; the power control module is used for receiving a remote control or program control command, and implementing the distribution and control of primary power of the microsatellite; the USB or spread-spectrum measurement and control module is used for implementing remote control and remote measuring functions of the microsatellite; the GNC module is used for performing attitude and orbit measurement and control on a GNC system; the driving control module is used for finishing driving control over micro variable structure solar wings, claws and the expansion of flexible solar wings; the data transmission module is used for receiving payload data and image data from the universal computer, finishing data management and packing processing, and downloading the data. The system has the characteristics of small size, lightweight, low volume cost, short development cycle, high performance and the like of the microsatellite.

The invention belongs to the field of microsatellite molecular markers and particularly relates to an acipenser dabryanus microsatellite marker as well as a screening method and application of the microsatellite molecular marker, wherein the acipenser dabryanus microsatellite marker has the nucleotide sequence of any one or more of SEQ ID No.1-8. In the invention, the development of microsatellite molecular markers using the high-throughput sequencing technology is faster and costs less than a traditional method; and moreover, the functional traits of corresponding EST sequence can be found while the microsatellite sites are obtained, and the gene relevance is relatively strong. In the invention, through the developed microsatellite sites, basic data is provided for the genetic diversity of acipenser dabryanus, and the study fields such as genetic diversity analysis, genetic map establishment, gene location, variety identification, germplasm conservation, quantitative character gene analysis and parent-child relationship identification can be carried out. According to actual needs, any one or more markers disclosed by the invention can be adopted, or any one or more pairs of specific primers corresponding to related markers can be analyzed and studied.

The invention belongs to the field of molecular biology DNA marking technology and application, in particular to a detecting method for a Litopenaeus vannamei Boone LvE165 microsatellite DNA marker. The invention provides a Litopenaeus vannamei Boone LvE165 microsatellite specific DNA primer, a PCR (Polymerase Chain Reaction) system utilizing the primer and a detecting method for the Litopenaeus vannamei Boone LvE165 microsatellite DNA marker. The method comprises the following steps of: extracting a Litopenaeus vannamei Boone genome and diluting for later use; designing specific primers at both ends of a sequence by utilizing a Litopenaeus vannamei Boone LvE165 microsatellite DNA core sequence; and then carrying out PCR amplification on genome DNA of different groups of Litopenaeus vannamei Boone or individuals in the group by using the primer, analyzing a product and confirming the genotype of each individual so as to obtain a polymorphism genetic variation map. The invention is mainly applied to Litopenaeus vannamei Boone germplasm resource and genetic diversity analysis, molecular population genetics, construction of the genetic map, and the like.