The invention discloses a quadruple fluorescent SSR molecular marker detection method for a tea tree. According to the invention, a set of detection method for quadruple SSR molecular markers in the tea tree is established by the combination of a primer combination, a touchdown PCR (polymerase chain reaction), capillary fluorescence electrophoresis and other technologies, and the method can not only improve the research efficiency and accuracy of the SSR molecule markers in the tea tree, but also save labor. However, the current detection of the SSR molecular markers in the tea tree mainly adopts a polyacrylamide gel electrophoresis technology, which has the disadvantages of low resolution, large error, low efficiency and the like; and by adopting the quadruple SSR molecular marker detection method provided by the invention, the detection of 4 pairs of SSR primers can be completed at a time. At the same time, the defects of primer annealing temperature screening, manual running rubber,artificial reading tape and the like are avoided, not only is the work efficiency greatly improved, but also the method is simple, rapid, accurate, stable and good in repeatability, and can be used for the research of tea tree variety identification, fingerprint mapping construction, population genetic structure, genetic differentiation and the like.