14 results about "Genetic differentiation" patented technology

The invention discloses an SSR (Simple Sequence Repeat) primer composition for evaluating genetic diversity of channel catfish breeding population and an application method of the SSR primer composition, and belongs to the technical field of fish genetic breeding. The method comprises the following steps: modifying 5'ends of forward primers of the primers by using FAM fluorophores respectively, amplifying genome DNA of each breeding group by using the 10 pairs of microsatellite site primers respectively, analyzing amplification products, reading genotypes, analyzing parameters, and determining the genotype of the breeding group. The analysis result is used for evaluating the population polymorphism, the variation condition of the population genetic structure and the genetic differentiation degree among the populations. By applying the evaluation method disclosed by the invention, the population genetic diversity and genetic structure of continuous breeding generations of channel catfish can be systematically evaluated. The SSR primer and the genetic diversity evaluation method can be used for genetic improvement and new variety breeding of channel catfish.

The application discloses a kind of integrated hybrid information purebred genome prediction method, belong to animal genetic breeding and propagation technical field.The population genetic dataset comprising purebred population A, B and hybrid population CB is constructed, and the population genetic differentiation is verified by principal component analysis and genetic differentiation index;After the genotypes of purebred and hybrid population CB are phased, the ancestral information inference method is used to determine the origin information of each allele and process missing sites;Based on the origin information of allele, additive effect partial kinship correlation matrix and dominant effect partial kinship correlation matrix are constructed;The above matrix is integrated into three-trait mixed linear model, forming the prediction model that simultaneously incorporates purebred and hybrid population genetic information;The application distinguishes the breed origin of allele and quantifies its additive and dominant effect, significantly improves the prediction accuracy of purebred genome breeding value, is suitable for the traits difficult to directly determine in purebred population, and provides reliable technical support for animal breeding.

The application provides a method for determining salmon run time based on genetic distance and principal coordinate analysis, and belongs to the technical field of molecular biology. On the basis of obtaining salmon microsatellite data, the application makes accurate determination of salmon run time at the molecular level by using a genetic differentiation coefficient in combination with a technical means of principal coordinate component analysis. Based on the application, technical support is provided for accurate determination of the run time of anadromous fish, and the application has important significance for the study of anadromous fish.

The invention provides a molecular marker linked with a major QTL (Quantitative Trait Loci) of the total grain number of a single rice plant and application of the molecular marker. The molecular marker is TGN5. A new major QTL TGN5 for controlling the total grain number of a single plant of the super rice Y900 is identified, is positioned in a section of about 1.46 Mb of a long arm of a chromosome 5, and just falls in a hot spot region Chr5: 17.9-27.3 Mb range of indica-japonica genetic differentiation of parents; the TGN5 is reduced to an interval of about 251.39 Kb by constructing a local genetic linkage map, and 13.3% of phenotypic variation is explained to the maximum extent; the loci are verified in segregation populations of the super rice Y1 and Y2 at the same time, and the single plant total grain number heterosis of the three-stage super rice can be differentially influenced. The molecular marker provided by the invention can be applied to regulation and control of the total grain number of a single rice plant.

The invention provides a 20K liquid phase chip for simultaneously and accurately identifying various goose varieties and application, the 20K liquid phase chip covers 22005 SNP molecular markers, and the SNP molecular markers are specifically composed of a genetic differentiation index FSTgt, a genetic differentiation index FSTgt, a genetic differentiation index FSTgt, a genetic differentiation index FSTgt, a genetic differentiation index FSTgt, a genetic differentiation index FSTgt, a genetic differentiation index FSTgt The SNP molecular marker is composed of 4991 genetic differentiation specific sites, 12 important character associated sites and 17002 whole genome background sites of 22005 goose varieties, and the specific positions of the 22005 SNP molecular marker sites are shown in a table 1 in the specification. According to the method, 16 goose varieties can be accurately distinguished, the identification efficiency is high, the detection process can be completed within 24 hours, 48-96 samples can be detected at a time, and the technical repetition rate is gt; the cost of the chip is far lower than that of a high-density chip, and the chip is suitable for carrying out multi-variety and large-scale germplasm monitoring and identification work in a breed conservation field, a research and development unit and the like, and is high in practicability.

The application belongs to the technical field of animal molecular marker, and discloses a SNP marker combination for identifying Diqing Tibetan pigs. Based on whole genome resequencing data of Diqing Tibetan pigs and other pig breeds, sample quality control and population structure analysis are carried out to determine the core samples with clear genetic background. On this basis, the deletion rate of whole genome SNP sites is filtered, the polymorphism is filtered, and the linkage disequilibrium is filtered to reduce redundant sites. Further combined with the difference of allele frequency and genetic differentiation index, a specific SNP site set with stable allele frequency distribution in Diqing Tibetan pig population and significant difference from other pig breeds is screened to obtain 57 SNP sites. Compared with the traditional method depending on appearance characteristics or pedigree record, the application has the advantages of high sensitivity, convenient operation and low detection cost, and is suitable for breed verification in Diqing Tibetan pig germplasm resource protection and production management.

The invention discloses a preparation method of a magnetic targeting ultra-small Fe3O4 nanoprobe SPIONPs, relates to the field of immune targeting biomedical diagnosis and treatment, and adopts S1 to prepare a NaOH solution and S2 to prepare a triton solvent. And S3, triton drops by drops. Heating is performed; s5, weighing FeCl3. 6H2O and FeCl2. 4H2O according to a molar ratio to prepare a solution; S6, slowly dropwise adding the mixed solution into the NaOH solution; according to the preparation method, magnetic nanoparticles with the particle size of 2-30 nm are prepared, an ultra-small Fe3O4 nanoprobe SPIONPs realizes loading functionalization, and the obtained magnetic targeting nanoparticles and probes subjected to surface modification functionalization modification treatment have the characteristics of long acting, stability, high efficiency, simplicity in preparation and high yield. Particularly, the invention has important application in the fields of immune targeted biomedical diagnosis and treatment, magnetic imaging, nuclear magnetic resonance enhanced imaging, gene editing, gene protection, gene differentiation, nuclear magnetic contrast fluorescence microscopy and targeted immune medical treatment.

The invention is suitable for the technical field of computational biology and parasitic molecule epidemiology, and provides a whole genome machine learning method and system for toxoplasma gondii population structure analysis. According to the method, an SNP genotype probability matrix is constructed by using whole genome sequencing data, an optimal group structure is identified in combination with unsupervised clustering, and then multi-site sequence typing and rapid classification prediction of toxoplasma gondii are realized through supervised tag integration and supervised learning models. According to the method, a closed-loop mechanism of'unsupervised clustering-semi-supervised labeling-supervised learning 'is formed, and interpretability and expandability are achieved while high precision (the average accuracy is about 97%) is kept. According to the method, core information sites are obtained through screening based on progressive feature selection of feature importance, high robustness and accuracy can still be kept under the background of low sequencing depth and metagenome mixing, support is provided for genetic differentiation evaluation, epidemiological traceability and the like, modules are clear and easy to integrate, and efficient landing is achieved.

This invention relates to a molecular marker for polymorphic SNPs in the Yangtze finless porpoise, its development method, and its application. Belonging to the field of DNA molecular marker technology, the nucleotide sequences of the polymorphic SNP molecular marker sites in the Yangtze finless porpoise are shown in SEQ ID NO:1-NO.19, and the primers used to amplify the SNP molecular marker sites are shown in Snpp 01-57. The SNP molecular markers provided by this invention are all derived from high-quality chromosome-level genomes of the Yangtze finless porpoise and are screened using resequencing data from three geographical populations. This allows for a more comprehensive detection of polymorphic SNP sites in the Yangtze finless porpoise genome. By utilizing KASP genotyping technology, genotyping of polymorphic SNP sites in the Yangtze finless porpoise can be performed efficiently and accurately, significantly reducing detection costs. This provides effective genetic molecular markers for population genetics studies such as genetic diversity assessment, genetic differentiation analysis, and kinship identification of the Yangtze finless porpoise and its closely related species.

The rate of evolution may be estimated for any length of genomic fragments (or amino acid sequences) from a single codon (or amino acid position) to the entire genome or protein. Under the condition that a target genome reference sequence at initial time and each genome specific sequence of one or more target strains within a certain time period after the initial time are given, some genome fragments can be selected; a genetic differentiation parameter for a reference sequence fragment may then be calculated based on the genetic distance between the fragment and a corresponding fragment in each strain-specific sequence. Based on the genetic differentiation parameters, the evolution rate or evolution stability parameter of the fragment may be calculated and applied to the design of antiviral therapy or antiviral vaccines and / or the estimation of tumor growth.

The present invention provides a microfluidic system and method for reprogramming, proliferating, preserving, and optionally differentiating cells. [Solution] An integrated microfluidic system is provided that utilizes microfluidic chip technology to accept a patient sample containing cells, grow the cells, reprogram the grown cells, and then store the reprogrammed cells in a microfluidic chip. These microfluidic chips containing the reprogrammed cells can then be used in situations of genetic differentiation to specific cell types. Overall, this system and workflow is suitable as a hospital-based device that enables the creation of any patient-derived iPSC for downstream diagnostic or therapeutic applications.

The invention discloses a picea genetic diversity analysis method, and relates to the technical field of forest tree genetic analysis, and the method comprises the steps: firstly obtaining two-dimensional space coordinates and genotype data of a picea sample in a replanting patch, determining a strip extension direction, and generating a one-dimensional projection coordinate; then constructing a parameter search space containing candidate stripe width and initial phase, traversing and searching an optimal stripe structure parameter which maximizes the genetic differentiation index between the virtual alternating subgroups; and finally, on the basis of the optimal grouping, calculating an in-band diversity reference value for removing the mixing effect, and correcting observation diversity parameters of the whole group by taking the in-band diversity reference value as a reference. According to the method, the recessive banded provenance structure in the complementary planting forest stand can be automatically identified, the Warrend effect deviation caused by neglect of a space structure is effectively eliminated, the problem of virtual high inbreeding coefficient in traditional evaluation is solved, and an accurate decision basis is provided for scientific conservation and operation of picea germplasm resources.

This invention discloses a genetic discrimination method for sedimentary bauxite based on gallium / lithium elemental distribution, belonging to the field of genetic discrimination technology. This method establishes a multi-dimensional geochemical index system, including gallium-titanium ratio, gallium weathering ratio, and lithium content, and solidifies specific quantitative threshold standards for three types of deposits: allochthonous weathering, in-situ sedimentary, and sedimentary-leaching altered deposits. It boasts high discrimination accuracy and effectively solves the technical problems of ambiguity, multiple solutions, and high misjudgment rate caused by traditional methods relying on field macroscopic observation and subjective experience. Furthermore, based on cluster analysis and discriminant analysis algorithms, it solves for the optimal classification interface in the multi-dimensional parameter space, compiling a gallium-lithium dual-element collaborative discrimination chart and a regionally specific genetic type quantitative standard table, significantly improving the scientific rigor of bauxite mineralization regularity research and the efficiency of mineral exploration.