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Screening method and application of Bian chicken molecular marker

A screening method and molecular marker technology, applied in the field of genetics, can solve the problems of not being able to objectively evaluate the conservation effect of border chickens, restricting the development and utilization of border chicken breed resources, poor accuracy, etc., achieving abundant data, low cost, Apply a wide range of effects

Active Publication Date: 2019-02-01
JIANGSU INST OF POULTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the evaluation of the conservation effect of border chickens mainly depends on conventional phenotypic trait records, pedigree records and low-density DNA molecular marker information, with low throughput and poor accuracy, which cannot objectively evaluate the conservation effect of border chickens; Development and Utilization of Liaobian Chicken Variety Resources

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  • Screening method and application of Bian chicken molecular marker
  • Screening method and application of Bian chicken molecular marker
  • Screening method and application of Bian chicken molecular marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Example 1 Screening of Chicken Molecular Markers

[0060] (a) Blood sample collection: Bian chicken conservation group and other breed conservation groups came from the national local chicken breed gene bank of Jiangsu Institute of Poultry Science, and 20 individuals (10 males and 10 females) were selected for each group according to the family line. ), there is no relationship between the individuals. 1 mL to 1.5 mL of blood was collected from the wing vein, anticoagulated with sodium citrate (ACD), and stored at -20°C for later use.

[0061] (b) DNA sample acquisition: Genomic DNA of all species was extracted by conventional phenol-chloroform method. Quality inspection of the obtained DNA, including nanodrop preliminary detection of DNA sample concentration; electrophoresis detection of DNA integrity, including whether DNA is degraded, whether there is contamination by other impurities such as protein and RNA; Qubit 2.0 accurately quantifies DNA samples and selects t...

Embodiment 2

[0071] The application of embodiment 2 chicken molecular markers

[0072] (a) Individual blood collection of Bian Chicken Bao population: use medical disposable syringes to collect 0.5-1.0ml of venous whole blood from male and female chicken wings randomly selected from Bao population (no less than 10 roosters and no less than 20 hens), Immediately after collection, inject 2 μL of 0.5mol / L Na 2 EDTA anticoagulant in an enzyme-free tube, and then store the enzyme-free tube at 4°C for future use.

[0073] (b) DNA extraction and quality inspection: suck out 0.2 to 0.3 ml of male and hen individual blood preserved in enzyme-free tubes at room temperature, and extract individual blood DNA using conventional animal peripheral blood phenol-phenol extraction; agarose gel The DNA integrity was analyzed by electrophoresis, and the DNA purity was detected by a spectrophotometer; qualified samples were stored at -80°C for SNP detection.

[0074] (c) SNP marker detection of chicken breed...

Embodiment 3

[0086] Application of Example 3 Chicken Molecular Markers in New Strain Breeding

[0087] On the basis of screening out the genes of the characteristics of the chicken breeds, we further analyzed the SNPs of each of the above genes.

[0088] (a) Individual blood collection of Bian Chicken Bao population: use medical disposable syringes to collect 0.5-1.0ml of venous whole blood from male and female chicken wings randomly selected from Bao population (no less than 10 roosters and no less than 20 hens), Immediately inject 2μL of 0.5mol / L Na 2 EDTA anticoagulant in an enzyme-free tube, and then store the enzyme-free tube at 4°C for future use.

[0089] (b) DNA extraction and quality inspection: suck out 0.2 to 0.3 ml of male and hen individual blood preserved in enzyme-free tubes at room temperature, and extract individual blood DNA using conventional animal peripheral blood phenol-phenol extraction; agarose gel The DNA integrity was analyzed by electrophoresis, and the DNA pur...

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Abstract

Relating to the field of genetics, the invention provides a screening method and application of a Bian chicken molecular marker. The screening method of a Bian chicken molecular marker includes: conducting high-throughput sequencing detection on SNP marker, calculating genetic statistics, analyzing and comparing the genetic difference of Bianji group and other chicken breed groups, and screening out a Bian chicken molecular marker. The method provided by the invention alleviates the technical problems of high Bian chicken genetic differentiation research cost and low accuracy caused by lack ofefficient molecular biological method in existing Bian chicken breed conservation effect evaluation process, and reaches the technical effect of efficient and rapid screening of a molecular marker for Bian chicken breed conservation effect evaluation. The invention also provides a screening method of the Bian chicken molecular marker and application of the screened molecular marker, and alleviates the technical problems of lack of efficient molecular biological marker and screening method in Bian chicken research.

Description

technical field [0001] The invention relates to the field of genetics, in particular to a method for screening molecular markers of chicken and its application. Background technique [0002] Domestic poultry breeds are precious resources endowed by the natural ecological environment and different national cultures for thousands of years. During the breeding and domestication process for thousands of years, many excellent characteristics have gradually formed. Bian chicken is a special local breed in my country. It has the excellent characteristics of large body, heavy eggs, good meat quality, strong adaptability, roughness and cold resistance. It is native to the inner and outer areas of the Great Wall adjacent to the northern part of Shanxi Province in the Inner Mongolia Autonomous Region. Because the local people regard the Great Wall as the "side wall", it is called side chicken. In recent years, with the impact of introduced breeds and the migration of people from mount...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/6869
CPCC12Q1/6869C12Q1/6888C12Q2600/124C12Q2600/156C12Q2535/122
Inventor 张会永朱静韩威李国辉朱云芬殷建玫苏一军盛中伟沈海玉
Owner JIANGSU INST OF POULTRY SCI
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