86 results about "Phenol extraction" patented technology

The invention discloses total phenyl propanoid extract and total phenol extract extracted from grassleaved sweetflag rhizome and its preparation method. Total phenyl propanoid extract mainly comprises beta -asaricin, alpha -asaricin, eudesmin, galgravin, veraguensin and other derivant with veraguensin as mother nuclide. Total phenol extract mainly comprises caffeic acid, protocatechuic acid, ferulic acid, vanillic acid and glucosides with vanillic acid as mother nuclide and other derivants. Total phenyl propanoid extract and total phenol extract of grassleaved sweetflag rhizome can be extracted through one or more of methods of solvent-extraction, solvent extraction, precipitation method, macroporous adsorbent resin, extraction by supercritical fluid, column chromatography, and liquid-liquid countercurrent distribution chromatography. Total percentage content of phenyl propanoids in the obtained total phenyl propanoid extract of Grassleaved sweetflag rhizome is 5-100% (w/w), wherein content of beta -asaricin and alpha -asaricin occupy 5-100% (w/w).Total percentage content of phenols in total phenol extract of grassleaved sweetflag rhizome is 5-100% (w/w), wherein content of caffeic acid and protocatechuic acid occupy 5-100% (w/w).

The invention discloses an extraction method for mangrove plant kandelia candel leaf total protein suitable for two-dimensional electrophoresis. A traditional trichloroacetic acid/acetone precipitation method for protein extraction and a traditional phenol extraction method for protein extraction are combined, a cosolvent SDS solution is introduced, the extraction process is optimized, and the Phe-B method suitable for extraction of the mangrove plant kandelia candel leaf total protein is established. The extraction efficiency and quality of the protein are effectively improved, and the technical scheme can be specifically suitable for extraction of the kandelia candel leaf protein in two-dimensional electrophoresis. The method has the advantages of being easy to operate, high in protein extraction efficiency and fewer in interfering substance, and is suitable for materials with difficult protein extraction, low protein extraction efficiency and more interfering substances. The extracted kandelia candel leaf protein completely meets the requirements of the first direction and the second direction of two-dimensional electrophoresis, a high-quality two-dimensional electrophoresis gel map with high resolution, more clear protein points, uniform distribution and the clear background can be obtained, and experiment repeatability and stability are good.

The invention relates to a high-value utilization method for oxygen-enriched coal tar. The method comprises the following steps of: first separating the oxygen-enriched coal tar into coarse phenol oil and dephenolization distillate oil in a phenol extraction unit, and performing hydrogenation reaction on the dephenolization distillate oil in a hydro-conversion unit to obtain an oxygen-enriched gas phase product and a hydrogenation liquid phase product in which hydrocarbon is taken as a main component; and performing alkali wash on the oxygen-enriched gas phase product to allow the oxygen-enriched gas phase product to enter the front end of the hydro-conversion unit and recycling, allowing the hydrogenation liquid phase product to enter a fractional distillation unit to separate out naphtha and diesel oil products, allowing tail oil to enter the hydro-conversion unit, and mixing the tail oil and the dephenolization distillate oil, wherein a mixture of tail oil and dephenolization distillate oil is used as a hydrogenation raw material. According to the method, the coal tar raw materials are subjected to high-value utilization, and the running cycle of the hydro-conversion unit can be prolonged.

The invention discloses a piece of complete equipment for extracting phenols in medium and low temperature coal tar, which belongs to the complete equipment for extracting the low temperature coal tar. The complete equipment for extracting the phenols in the medium and low temperature coal tar comprises five kettles, four tanks and three groups of condensers, which are respectively a medium and low temperature coal tar extracting and separating kettle, an extraction light oil No. 1 extractant concentration kettle, a No. 1 solvent tank, an extraction crude phenol No. 2 extraction agent concentration kettle, a No. 2 solvent tank, a No. 3 solvent refined crude phenol extraction kettle; an extraction refined crude phenol filter tank, an extraction refined crude phenol No. 3 extractant concentration kettle, a No. 3 solvent tank, a No. 1 condenser, a No. 2 condenser and a No. 3 condenser. The complete equipment disclosed by the invention can quickly, simply and selectively realize the refined separation of the low-carbon hydrocarbons and mixed phenols from the medium and low temperature coal tar in high purity, has an obvious condensing effect of the multi-level condensers, has little environmental pollution, can be easy to recycle and circularly use, and can easily realize the high-quality and high added value use, such as large-scale production, the separation and conversion of the low temperature coal tar and so on.

The invention relates to a method for processing low-temperature pyrolyzed coal tar. The method comprises the following steps of: pressurizing and standing low-temperature coal tar to be pyrolyzed, dehydrating initially by changing surface tension, adding a demulsifying agent to form azeotropic water through light oil circulation and performing final dehydration; extracting crude phenate from the dehydrated low-temperature coal tar by alkaline washing; purifying the extracted crude phenate through oil-water mixed gas and decomposing through carbon dioxide so as to obtain crude phenol; and dehydrating the obtained crude phenol, deslagging and rectifying so as to obtain a target product. By the method, the phenol extraction rate of the low-temperature pyrolyzed coal tar is over 95 percent, the problem of difficult oil and water separation can be solved and the dehydration rate after processing is between 0.5 and 1 percent. The method is a processing method which has low cost and reasonably utilizes a low-temperature coal tar resource.

The invention provides a polyphenol mixture recycling purification technology which comprises the steps of alkalization and phase splitting: heating a polyphenol mixture to react with a sodium carbonate solution to general phenol sodium salt, conveying reaction liquid into an alkalization and phase splitting tower for continuous reaction to generate the phenol sodium salt and phase splitting and obtaining a water phase rich in the phenol sodium salt and an organic light component, acidification, water washing and phenol extraction: conveying the water phase into an acidification tower for acidification, allowing an organic phase on the upper layer in the acidification tower to overflow into a water washing tower for washing treatment, ethanol crystallization mixing and adding the organic phase after the water washing and ethanol into a crystallizer for crystallization to form 2,3-dimethyl phenol, and rectification and separation: continuously conveying primary mother liquid into a primary rectification tower for rectification till a metacresol finished product with a content greater than 98% is generated continuously in the primary rectification tower, and conveying kettle liquid with a 3,5-dimethyl phenol content greater than 80% from the primary rectifying tower into a secondary rectification tower for continuous rectification and purification to form a product with the 3,5-dimethyl phenol content greater than 98%.

The present invention discloses an Indian strawberry phenol extraction extracted from an Indian strawberry whole grass, which also relates to the preparation method of the extraction and the function of the extraction. The preparation method of the Indian strawberry phenol extraction is as following, which comprises soaking and extraction of the Indian strawberry by water, ethanol, or a mixed solution with water and ethanol in a certain ratio; vacuum concentration of the Indian strawberry extraction liquid; then the ethanol is removed from the extraction liquid and passed through a malodorous resin column; the liquid is eluted by the ethanol after elution of the non affection component with the water; the ethanol elution liquid and vacuum concentration are collected, therefore the Indian strawberry phenol extraction is obtained. The Indian strawberry phenol extraction of the present invention possesses functions of prohibiting the generation and growth of various kinds of tumor cells. Therefore the present invention is applicable for treatment of a plurality of cancer diseases comprising the lung cancer, the liver cancer, the nasopharynx cancer, the gastric cancer, the cervical cancer, the endometrium cancer and the ovarian cancer, etc. In addition, the Indian strawberry phenol extraction in the present invention has active function of improving the immune ability in the organism.

The invention provides a process for purifying typhoid Vi polysaccharide. In the process, phenol is not used for removing protein in the purification process, so use of the phenol is avoided, the dialysis step after phenol extraction is not required, production time is shortened, and production efficiency is improved. The phenol is not used in the technical process, so vaccine does not contain the phenol, a vaccinated object does not contact with the phenol and is not damaged by the phenol, health of vaccinees is contributed, health of production staff and ecological environment are protected, and the defect that a great deal of phenol is used in the traditional process is overcome.

An apparatus for recovering petroleum and phenols from oilfield produced wastewater is formed by a wastewater equalization pool, a demulsification pool, a gravity settling pool or a centrifuge or an air floatation device, a petroleum storage tank, a wastewater collecting pool, a phenol adsorption tower, an eluant storage tank, a phenol-containing eluate storage tank, an adsorption tower dephenolized wastewater storage tank, a phenol extraction tank extractant storage tank, a distillation tower and a phenol storage tank. A recovery method adopting the apparatus comprises the following steps: 1, adding an acid to the oilfield produced wastewater to adjust the pH value to 2-6 in order to demulsify; 2, carrying out gravity settlement, centrifuge or air floatation separation on the demulsified oilfield produced wastewater to obtain recovered petroleum and deoiled or dewaxed wastewater; and 3, allowing the deoiled or dewaxed wastewater to go through the adsorption tower and to adsorb phenols in the wastewater, and adding a 5-10% sodium hydroxide solution or alcohol after absorption is saturated to elute in order to regenerate an adsorbent and recover phenols. Influences of phenol wastewater treatment on biochemical treatment are eliminated through recovering petroleum and phenols from the oilfield produced wastewater, and the apparatus and the method simultaneously realize treatment of pollution of the oilfield produced wastewater and recycling of resources.

The invention relates to a method for producing fuel oils by hydrogenation after coal tar cleaning and phenol extraction and a system thereof. According to the technical scheme, coal tar is sent into a distillation column to separate a phenol-rich fraction and other fractions; dephenolized oil obtained after cleaning and phenol extraction of the phenol-rich fraction is used as solvent naphtha to be mixed with a catalyst and a vulcanizing agent, and the mixture is vulcanized to prepare catalyst slurry; the other fractions and the catalyst slurry are mixed and the mixture is sent into a slurry-bed hydrocracking reactor to undergo a hydrocracking reaction; reactants obtained after the hydrocracking reaction are then sent into a separation system to separate hydrogen-rich gas, light oil and tail oil containing a catalyst; the light oil is sent into a fixed-bed hydrogenation reactor to undergo a hydrofining reaction; and reactants obtained after the hydrofining reaction are sent into a fractionating tower to separate gaseous hydrocarbon, a gasoline fraction and a diesel fraction. The technology of the invention is simple; utilization rate of raw materials is high; the system is environmentally friendly, has low production cost and operating cost and is simple to maintain; service life of a catalyst is prolonged; and product yield is high.

The invention discloses a Hib polysaccharide purifying technology which comprises eight steps of: collecting polysaccharide composite precipitate, centrifugally collecting the raw sugar precipitate, washing the raw sugar precipitate, drying the raw sugar, dissolving the raw sugar, performing phenol extraction, removing phenol and refining to obtain refined sugar. The invention provides a Hib polysaccharide purifying technology; each index of the Hib polysaccharide manually purified by the technology in the continuous production batch can meet the pharmacopeia standard at the intermediate level; the impurity residue amount is far lower than the standard limit value, and thus the product quality is improved; the operation steps and reagent usage amount are reduced, and the production of waste is correspondingly reduced, thereby being conducive to environmental protection; the purifying conditions are relatively mild, and the product is treated in a tight environment so as to meet the requirements of the existing GMP (good manufacturing practice) on the production process and protect the product from pollution; and the refining recovery rate of Hib polysaccharide can be improved by 20-30% over the traditional technology.

The invention discloses a method for extracting and purifying a columnar high-purity animal mtDNA. In the method, an alkaline denaturation method is taken as a basis and improved by adding a DNaseI digestion step to eliminate the residue of nuclear DNA, and by adding a RNase digestion step to eliminate the residue of RNA; a column chromatography is adopted to replace a phenol extraction method for purifying the mtDNA to solve the problems of phenol toxicity and residue thereof. The mitochondrial DNA obtained by the method has high purity; and electrophoresis detection displays that an electrophoresis band is clear, neat and even with clear background and without the situations of macromolecular DNAs near sample application holes, front-end DNA trailing, and the like.

The invention relates to a semi-coke high-concentration organic wastewater resource treatment system and method. The system comprises a pretreatment unit, an ammonia evaporation and phenol extractiontreatment unit and a fine filtration and adsorption treatment unit which are connected in sequence, wherein the pretreatment unit completes pretreatment on semi-coke high-concentration organic wastewater by heavy oil separation, multiphase flow separation, flocculation sedimentation, double medium filtration and high-precision coalescence oil-water separation, so that coal tar and mechanical impurities in the pretreated wastewater are treated to qualified indicators; the ammonia evaporation and phenol extraction treatment unit implements ammonia removal and phenol extraction on the pretreatedwastewater by using hot steam carrier heat transfer and countercurrent extraction for separating phenol oil and water; and the fine filtration and adsorption treatment unit performs fine filtration and deep phenol removal on the wastewater after the ammonia removal and phenol extraction. The system and the method can effectively treat the semi-coke wastewater, recover ammonia nitrogen, phenol andoil as byproducts from the semi-coke wastewater, greatly reduce the operating cost of an environmental protection device, and are particularly effective for later industrial application.

The invention discloses a preparation method of group A/C meningococcal polysaccharide. The preparation method comprises the following steps: (1) carrying out fermentation culture on meningococcus, and carrying out sterilization treatment, thus obtaining a sterilized culture solution; (2) preparing meningococcus capsular polysaccharide; and (3) dissolving the rough polysaccharide, carrying out chromatography elution, collecting target peaks containing the polysaccharide, carrying out desalting on the collected polysaccharide solution by adopting an ultrafiltration membrane and adopting water for injection as an ultrafiltrate, thus obtaining the desalted solution, namely, the group A/C meningococcal polysaccharide solution. According to the preparation method, firstly, sterilization treatment is carried out, so that the inactivated culture solution is obtained, then, the capsular polysaccharide is prepared, and then, the capsular polysaccharide is purified by adopting the chromatography method; the use of abundant phenol in the traditional purification method is avoided, on the basis that the purification quality is guaranteed, the destruction of phenol to the environment is effectively avoided, meanwhile, the recovery rate of polysaccharide is approximate to that of the traditional phenol extraction method, in addition, the operation is simple, and the preparation method is suitable for large-scale production.

The invention discloses a method for preparing triphenyl phosphate through phenol extraction in aryl phosphoric ester production waste water. The method comprises the following steps of adding acid into the aryl phosphoric ester alkali washing waste water for regulating the pH value to 6 to 7; stirring and still standing layering to obtain an oil layer and waste water with the phenol content beinglower than 25000 ppm; adding triphenyl phosphate extraction agents into the waste water for stirring extraction; transferring an extraction phase into a distillation kettle for reduced pressure distillation dewatering; putting the dewatered extracts into an esterification kettle; next, adding phosphorus oxychloride and anhydrous magnesium chloride for esterification reaction to obtain triphenyl phosphate coarse products; sequentially performing acid washing, alkali washing, water washing and distillation treatment to finally obtain the qualified triphenyl phosphate finished product. Through the unified use of the triphenyl phosphate with the most simple phenol reuse process and least links as the extraction agents, one set of equipment can be used for solving the phenol recovery problem of various aryl phosphoric ester waste water in a concentrated way.

The present invention discloses a broadbean root system proteomics analysis sample preparation method, which taking a fresh broadbean root system; grinding with liquid nitrogen; adding a phenol extraction liquid; adding a Tris balanced phenol saturated solution; carrying out centrifugation to collecting the upper layer phenol; adding 0.1 mol/L ammonium acetate-methanol solution; carrying out centrifugation to collecting precipitate; washing with methanol; carrying out centrifugation to collecting precipitate; carrying out vacuum drying on the precipitate; adding a sample lysate; carrying out centrifugation, and taking the supernatant; carrying out protein quantitation; sub-packaging, and freezing; carrying out sample hydration; and completing the sample preparation. According to the present invention, the broadbean root system proteomics analysis sample preparation method has advantages of simple experiment steps, easy operation, good repeatability, and easy studying and easy grasping by the technical staff having the experiment background; and the selected experiment reagents are the two-dimensional electrophoresis conventional reagents, and the expensive and rare reagents are not used, such that the experimental operation cost is low.

The invention discloses a novel method for removing protein impurities in groups A, C meningococcal capsular polysaccharide. According to the novel method, under the acidic pH condition, an ionic detergent sodium deoxycholate has characteristics of forming micelle and absorbing the precipitate protein and is utilized to separate capsular polysaccharide and the protein. Refined polysaccharides produced by the novel method all accord with pharmacopoeia requirements. Recovery rate of polysaccharide is similar to that of polysaccharide by a traditional cold phenol extraction method. In addition, the novel method provided by the invention has characteristics of simple operation, low production cost and no harm to production personnel and the environment, and is more suitable for large-scale production.

A coal tar processing and coal co-processing combined process includes steps: firstly, subjecting a coal tar light component to phenol extraction treatment; then, subjecting coal tar heavy distillateto prehydrogenation to promote conversion of colloid and asphaltene in heavy distillate into partially hydrogenated polyaromatic hydrocarbon, so that the hydrogen supply performance of the heavy distillate can be improved; finally, mixing liquid-phase products in different distillation ranges in a separator after hydrogenation with coal powder, and adding into a coal-oil co-processing device to obtain gasoline, diesel and phenol products, so that coal tar and coal co-processing is realized. By combination of coal tar phenol extraction, heavy distillate prehydrogenation and coal-oil co-processing processes, quality-based utilization of coal tar is realized, a great quantity of solvent oil with the great hydrogen supply performance is provided for coal-oil co-processing, the coal tar and coal conversion efficiency is remarkably improved, and the process has advantages of low hydrogen consumption, high oil yield, great economic benefits and benefits to long-period large-scale operation ofa coal-oil co-processing device and the like.

The invention relates to a method for extracting phenolic compounds in low-temperature coal tar by using ionic liquid. The method for extracting the phenolic compounds in the low-temperature coal tar by using the ionic liquid specifically comprises the following steps: (1) adding monoethanolamine formic acid ionic liquid in the low-temperature coal tar, and performing reaction to obtain mixed solution; (2) standing the mixed solution to enable two phases to be layered, transferring the subnatant into another container, adding ethyl ether into the container and standing for layering, wherein the subnatant is mixture of the ethyl ether and the phenolic compounds; (3) distilling out the ethyl ether to obtain the phenolic compounds. The method for extracting the phenolic compounds in the low-temperature coal tar by using the ionic liquid is simple and easy to operate, the environmental pollution is small and the phenol extraction rate highly reaches 98 percent.

The present invention discloses a method for purification of bacterial capsular polysaccharide at low temperature (<20° C.) by salt-induced two-phase aqueous micellar system with TritonX-114 and can significantly reduce the endotoxins. Impurity proteins and the endotoxins need to be removed as many as possible in the preparation process of bacterial capsular polysaccharide vaccine to reduce the side reaction of the vaccines. Currently, the phenol extraction method, column chromatography and ethanol precipitation method all have a number of drawbacks. As compared with the phenol, the nonionic detergent has the advantages such as non-corrosiveness and non-carcinogenicity, and does not cause harm to the environment. As compared with the column chromatography, the method of the present invention is large in throughput, time-saving, economical, and easily expanding the scale production, which is a safe, environment-friendly and efficient.

This invention relates to a kind of nm sensor and a method for testing the phenol concentration in the Roles of phanerochaete chrysosporium aerobic broth, which takes Au nm particles fixed on the surface of a sensor as the crystal seeds, utilizes the theory of catalyst increase of Au nm particles under the action of phenol recovery and the rule of absorption spectrum variance to compute the variety of the nm particle absorption peak value in the spectrum to test the phenol concentration. This invention takes the hydroquinol concentration as the total phenol level to be analyzed by ultraviolet-visual absorption spectrum and applies a scan mirror to express the surface variance of the sensor, in which, the linear sphere of the hydroquinol concentration is 7x10< -6 >-3.81x10< -4 >M and the test limit is 7x10< -6 >M.

The invention relates to a mouth wash with a function of resisting helicobacter pylori. The mouth wash is prepared from traditional Chinese medicine active components A, a filler B, a corrigent C anda solvent D, wherein the traditional Chinese medicine active components A comprise 0.05-0.1 part of rheum emodin extraction liquid, 0.05-0.1 part of berberine extraction liquid, 0.1-0.2 part of sophocarpidine extraction liquid, 0.2-0.4 part of baicalin extraction liquid and 0.3-0.7 part of mango ginger combined phenol extraction liquid. The mouth wash can effectively kill the helicobacter pylori and inhibit colonization of the helicobacter pylori.

The invention discloses a method for extracting proteins from rubber trees and sesuvium portulacastrum plants by a phenol extraction method. The method for extracting proteins from rubber trees and sesuvium portulacastrum plants comprises the following steps: collecting plant tissues, grinding tissue samples in a liquid nitrogen environment to obtain plant tissue powder; adding BPP extraction buffer solution to the plant tissue powder, carrying out mixed centrifugal treatment to obtain mixed solution, taking the supernatant of the mixed solution, adding a precipitant to the supernatant in 1:5of volume ratio of the supernatant to the precipitant, carrying out precipitation at -20 DEG C overnight to obtain plant protein precipitates, and washing the plant protein precipitates to extract proteins. According to the method of the present invention, proteins can be extracted from plants with polysaccharides and polyphenols or halophyte plants by combining different precipitants, and proteins in low-concentration solution can be extracted. The method of the present invention is suitable for extracting proteins from plants with polysaccharides, polyphenols and high salt content, and can also be used for obtaining proteins in high yield from recalcitrant plants.

A method for preparing fuel oil by co-processing of solid waste and coal tar includes: (1), extracting phenol of light components of coal tar; (2), subjecting heavy distillate of coal tar to prehydrogenation to promote the process of converting gelatine and asphaltene of heavy distillate into partially hydrogenated polycyclic aromatic hydrocarbon so as to improve hydrogen supply performance of theheavy distillate; (3), mixing liquid-phase products with different boiling ranges with the solid waste in a separator after the hydrogenation so as to obtain a mixture, and feeding the mixture in a co-processing reaction device to obtain gasoline, diesel oil and phenolic products, thereby realizing co-processing of the solid waste and coal tar. According to the method, the process of phenol extraction to the coal tar and the co-processing of hydrogenated heavy distillate and the solid waste are combined, a great quantity of solvent oil with good hydrogen supply capacity is provided for liquefying treatment of the solid waste, molecular utilization of the coal tar, and scaled, clean and effective utilization of the solid waste are both achieved, conversion efficiency of the coal tar and the solid waste is increased greatly, and the method has the advantages of needing low hydrogen consumption, having high oil yield and good economic benefit, and facilitating long-cycle scaled running of device, and the like.

The invention relates to the field of biotechnology, in particular to a method for extracting fermented grain RNA rapidly and efficiently. The method comprises three steps of pretreating a fermented grain sample, extracting the total RNA of the fermented grain sample and removing the DNA from the total RNA of the sample. The method provided by the invention has the following beneficial effects: the fact that the microbial total RNA is extracted by a hot phenol method in a Baijiu making process is proposed for the first time; by the pretreatment step, microorganisms in the fermented grains anddistiller's yeasts can be obtained comprehensively; saccharose-containing buffer liquid is adopted in the extraction step, so high-quality RNA can be obtained; and 1/10 volume of 3 mol/L sodium acetate with the pH being 5.2 is added before acid phenol extraction, so RNA and protein layering is benefited. The method provided by the invention is low in cost, convenient and rapid in operation, avoidsenvironmental pollution and has small harm to a human body; furthermore, the RNA can be extracted from the fermented grains rapidly and efficiently, and the method is of great importance for researchon microbial total RNA extraction in the Baijiu making process.

The invention discloses a phenolic wastewater biological treatment device. The phenolic wastewater biological treatment device comprises a raw liquid supply tank used for containing phenolic wastewater, a biological reaction component connected with the raw liquid supply tank, a phenol extraction part connected with the biological reaction component, a separation device which is connected with the phenol extraction part and separates out solution and water and a pump component providing flowing power for the solution; the solution separated out by the separation device flows into the biological reaction component. The invention provides a biological treatment device which can continuously and efficiently remove phenol difficult to degrade in industrial wastewater, water is effectively improved, and phenol and an extraction agent are recycled, so that the effects of environment conservation and cost reduction are achieved.