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Response surface method for extracting Solidago canadensis antioxidant component

An anti-oxidant component and response surface method technology, which is applied in the direction of medical preparations containing active ingredients, plant/algae/fungus/moss components, anti-toxins, etc., can solve the problems of low precision, low extraction efficiency, unfavorable production industrialization, etc. Problems, to achieve the effect of high precision and efficiency, high clearance rate

Inactive Publication Date: 2015-08-12
JIAXING VOCATIONAL TECHN COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the phenolic antioxidant components of Solidago canadensis have strong anti-oxidation and free radical scavenging effects, and can also delay or prevent the oxidation of other nutrients. However, the extraction process of phenolic components in Solidago canadensis is currently mainly Orthogonal optimization method is used for extraction, and the extraction efficiency is too low, which is not conducive to the industrialization of production
For example, Guo Jie mentioned the optimal extraction method using the orthogonal experiment method in the article "Research on the phenolic components and biological activities of Solidago canadensis" published in the master's degree thesis of Yangzhou University. The orthogonal experiment method can screen out the significant influence indicators factors and give the best level combination of factors, but there are disadvantages that the best combination of factors and response values ​​in the entire area cannot be found, and linear models are mostly used, which deviate from the actual situation and the accuracy is not high.

Method used

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  • Response surface method for extracting Solidago canadensis antioxidant component
  • Response surface method for extracting Solidago canadensis antioxidant component
  • Response surface method for extracting Solidago canadensis antioxidant component

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Effect of Extraction Solvent on Extraction Rate of Total Phenols and OH Scavenging Rate

[0052] Weigh five parts of Solidago canadensis pollen, each 1.0000g, and then use a Soxhlet extractor to degrease; add the degreased Solidago canadensis pollen to the solvent to make the liquid-solid ratio 20:1, and control the temperature at 40 Ultrasonic extraction was carried out under the condition of ℃ for 30min, the power of the ultrasound was 100W, and the solvents were 60% acetone aqueous solution, 60% ethyl acetate aqueous solution, 60% ethanol aqueous solution, 60% methanol aqueous solution and ultrapure water respectively 20.00mL, to investigate the effect of solvent on the extraction rate of total phenols and the scavenging rate of OH. Such as figure 1 As shown, when acetone is used as the extraction solvent, the concentration of total phenols ( figure 1 The ability of adopting phenolic concentration to express in) and OH scavenging rate is all stronger, therefore, th...

Embodiment 2

[0054] The effect of the volume fraction of acetone on the extraction rate of total phenols and the scavenging rate of ·OH

[0055] Weigh six parts of Solidago canadensis pollen, each 1.0000g, use a Soxhlet extractor and carry out reflux degreasing treatment in petroleum ether, add volume fractions of 40%, 50%, 20.00mL of 60%, 70%, 80% and 90% acetone aqueous solution (volume fraction) was ultrasonically treated at 40°C for 30min with an ultrasonic power of 100W to investigate the effect of acetone concentration on the extraction rate of total phenols and the scavenging rate of ·OH. Specific findings such as figure 2 As shown, with the increase of acetone concentration (acetone volume fraction), the OH scavenging rate decreased, while the total phenol extraction rate fluctuated. When the acetone volume fraction was 40% to 60%, the total phenol extraction first decreased and then increased; When the volume fraction of acetone is greater than 60%, the extraction rate of total ...

Embodiment 3

[0057] Effect of Liquid-Solid Ratio on Extraction Rate of Total Phenols and ·OH Scavenging Rate

[0058] Weigh six parts of Solidago canadensis pollen, each 1.0000g, use a Soxhlet extractor and carry out reflux degreasing treatment in petroleum ether, respectively according to the liquid-solid ratio of 10:1, 20:1, 30:1, 40: 1. Add 40% acetone aqueous solution at 50:1 and 60:1, ultrasonicate at 40°C for 30 minutes, and the ultrasonic power is 100W, to investigate the effect of liquid-solid ratio on the yield of total phenols and hydroxyl scavenging rate, the specific results are as follows image 3 As shown, with the increase of the liquid-solid ratio, the yield of total phenols showed an increasing trend, and the OH clearance first decreased and then increased. When the liquid-solid ratio was greater than 30:1, the OH clearance rate began to increase, and the liquid-solid ratio When it is 60:1, although the total phenol extraction rate and OH clearance rate are high, the amoun...

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Abstract

The invention relates to a method for extracting antioxidant components of solidago canadensis L. by response surface method, which belongs to the field of plant extraction technology and aims to solve problems of the existing orthogonal extraction method, such as low extraction efficiency and low accuracy. The method comprises the steps: pulverizing Solidago canadensis L. pollens, and degreasing; adding acetone-water mixed solvent, ultrasonic extracting, and setting variable parameters X1, X2 and X3 by response surface method, wherein X1 represents acetone volume fraction, X2 represents ultrasonic time, and X3 represents liquid-to-solid ratio; centrifuging after ultrasonic treatment to obtain extract liquid containing the antioxidant components. The method provided by the invention has the advantages of high accuracy and high efficiency; and the extract liquid has the advantages of high total phenol extraction rate and high OH free radical scavenging rate.

Description

technical field [0001] The invention relates to a method for extracting antioxidant components of solidago canadensis by using a response surface method, and belongs to the technical field of plant extraction. Background technique [0002] Solidago Canadensis L., also known as Bawanghua, is a perennial herb native to North America. It was introduced into my country as a garden ornamental plant in 1935, especially in Shanghai and Nanjing, and later it also escaped in the wild. Solidago canadensis reproduces through rhizomes and seeds, which has strong fecundity, fast propagation speed, obvious growth advantages and wide ecological adaptability, thus posing a serious threat to biodiversity. [0003] On the other hand, the medicinal parts of Solidago canadensis are mainly branches, leaves and medicinal sequences, which contain a large amount of saponins and flavonoids. At present, the research on chemical components is mostly focused on polyynes, terpenes, saponins and flavonoi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/28A61P39/06
Inventor 陈凌
Owner JIAXING VOCATIONAL TECHN COLLEGE
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