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Method for extracting protein and chitin from fly maggot by using enzyme hydrolysis as well as preparing chitosan from chitin

A technology of fly maggot chitin and hydrolyzed protein powder, which is applied in the field of biotechnology enzymatic hydrolysis of fly maggot protein, can solve problems such as large waste of protein resources, pollution in production process, complicated processing, etc., achieve important economic and social benefits, improve Storage safety, the effect of improving nutritional value

Inactive Publication Date: 2003-05-07
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the high protein content in the maggot skin obtained by the mechanical method, the follow-up processing is complicated, the processing time is long, the waste of protein resources is large, and the production process is seriously polluted.
More importantly, there is no effective way to remove the black mouth and yellow viscera during processing, which affect the degree of polymerization, color and impurity of chitin and chitosan products, resulting in a decline in product quality
[0004] Find out from relevant literature, though existing fly maggot is used for the open literature report of animal protein powder formula and as animal protein feed additive, and existing from fly maggot extracts the open literature report of fly maggot chitin with chemical method, as Chinese patents 971164908, 981034462, 991193911, 001002651, etc., but there are no reports of extracting protein from fly maggots and recovering chitin by enzymatic hydrolysis at home and abroad

Method used

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  • Method for extracting protein and chitin from fly maggot by using enzyme hydrolysis as well as preparing chitosan from chitin
  • Method for extracting protein and chitin from fly maggot by using enzyme hydrolysis as well as preparing chitosan from chitin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Hydrolyzed protein and chitin were extracted from fly maggots by enzymatic hydrolysis. Fresh fly maggots are crushed by rubber mill, and the slurry is heat-treated, then hydrolyzed for 3 hours with AS.1398 protease at an enzyme-to-substrate ratio of 500u / g, 45°C and pH 7.0. At this time, the degree of hydrolysis was 18%, the dissolution rate of protein was 81%, and the hydrolyzate had no bitter taste.

[0042] The enzyme hydrolyzate is used to inactivate the enzyme, filtered, and the filtrate undergoes Maillard reaction. When the reaction is carried out at 120°C for 60 minutes, the product has a strong fragrance and the fishy smell disappears. Spray drying to obtain fly maggot protein powder.

[0043] The filter residue of fly maggot skin is used to extract chitin, and the method is the same as in Example 4 below.

Embodiment 2

[0045] Hydrolyzed protein and chitin were extracted from fly maggots by enzymatic hydrolysis. Using AS.1398 protease and flavor enzyme, the two are combined with a 1:1 (w / w) compound enzyme for enzymolysis, and the rest of the conditions are the same as in Example 1. The degree of hydrolysis and protein dissolution rate after hydrolysis can also achieve satisfactory results.

Embodiment 3

[0047] Hydrolyzed protein was extracted from fly maggots by enzymatic hydrolysis. After the fly maggots are enzymatically hydrolyzed, the enzymatic hydrolyzate is directly enriched by Maillard reaction, spray-dried to obtain fly maggot protein powder, and the protein content is more than 60%.

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Abstract

A process for extracting protein and chitin from fly maggot and preparing chitosan from the chitin incldues such steps as extracting hydrolytic protein by enzyme hydrolysis, removing stink and enhancing fragrance by Maillard reaction, enzymolyzing, removing yellow substances and black spot, etc. The quality of produced protein and chitin conforms with food grade standard.

Description

technical field [0001] The invention relates to a method for extracting protein and chitin from fly maggots, in particular to a method for extracting protein and chitin from fly maggots by enzymatic hydrolysis and preparing chitosan from chitin, which belongs to biotechnology enzymatic hydrolysis Field of fly maggot protein technology. Background technique [0002] Fly maggot is an insect with fast reproduction speed and high nutritional value, which contains high-quality protein and chitin resources. At present, the level of large-scale breeding of fly maggots has been formed. But fly maggot is mainly used as alternative feed at present, and the technical content of product is low, and economic benefit is poor, has seriously hindered the development of fly maggot breeding industry. In recent years, with the in-depth understanding of the nutritional value and functional activity of fly maggots, the use of fly maggots has shifted from alternative feed to in-depth developmen...

Claims

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Application Information

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IPC IPC(8): C12P19/04C12P21/00
Inventor 夏文水陈洁项建琳贺继东
Owner JIANGNAN UNIV
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