490 results about "Chitin" patented technology

The invention belongs to the technical field of biomedical materials, and particularly relates to a multi-layer multifunctional hydrogel microsphere for diabetic wounds as well as a preparation method and application of the multi-layer multifunctional hydrogel microsphere. The preparation method comprises the following steps: firstly, synthesizing a chitosan derivative with proper amino and aldehyde group contents, oxidized macromolecules and modified chitin; then preparing photocuring hydrogel microspheres by utilizing a microfluidic technology, and sequentially immersing the photocuring hydrogel microspheres into an oxidized polymer solution, a modified chitin solution, a tannic acid solution and a metal salt solution, so as to construct the hydrogel microspheres with a multifunctional layer structure. The invention further provides specific application intervals of parameters such as solvents used by various solutions, concentration ranges and soaking time. The preparation method provided by the invention has expansibility and universality, various types of microspheres can be constructed for sequential release of bioactive substances, and the requirements of tissue repair are met.

The invention belongs to the technical field of green processing of biomass materials, and particularly relates to a green preparation process of high-deacetylation-degree chitin. The preparation method aims at the problem of high energy consumption of existing high-deacetylation-degree chitin preparation. According to the method, raw materials are pretreated by utilizing a compound enzyme system under a low-temperature condition, chitin crystal lattices are preliminarily dissociated by combining a deep eutectic solvent at a medium temperature, high deacetylation conversion is realized through short-time dissolution of an ionic liquid, and meanwhile, a membrane separation-electrodialysis-gradient freezing crystallization three-stage recycling technology is matched, so that recycling of the solvent is realized; the method greatly reduces the reaction temperature and energy consumption, avoids the use of corrosive reagents, and has the advantages of environmental protection, low energy consumption, high deacetylation degree of the product, excellent quality and the like.

The invention discloses a preparation method and application of crab shell chitin without chemical decolorization, and the preparation method comprises the following steps: crushing the crab shell of Eriocheir sinensis to obtain crab shell powder; dissolving crab shell powder in distilled water according to a ratio of 1: 10-1: 30 (w / v) to obtain a crab shell powder solution; treating the crab shell powder solution by using plasma jet (PJ), washing to be neutral, and drying to obtain plasma pretreated crab shell powder; dissolving the crab shell powder pretreated by the plasma into 0.1-3M hydrochloric acid (HCl) solution according to the ratio of (1: 10)-(1: 30) (w / v), and carrying out demineralization treatment; washing the demineralized solid matter to be neutral, performing deproteinization treatment by using 0.1-3M sodium hydroxide (NaOH) solution, washing to be neutral, and drying to obtain white solid crab shell chitin. In the extraction process of the crab shell chitin, a strong oxidant (such as H2O2) is not required to be used for decoloring, the color is close to that of commercial chitin, and the process is simple, green and environment-friendly; meanwhile, the production cost is reduced.

The invention relates to the field of water treatment, in particular to a composite material with polyfunctional groups and a preparation method and application thereof.The composite material with the polyfunctional groups contains at least two groups including carboxyl, amino, amido and hydroxyl and at least one group including sulfo and nitro; the composite material contains a cross-linked compound of a polymer I and a polymer II, and the polymer I is a carbon chain polymer and contains at least one group including a sulfonic acid group and a nitro group; and the polymer II is at least one of chitosan and a derivative thereof, and chitin and a derivative thereof. The composite material with multiple functional groups is preferably a composite fiber, has a synergistic adsorption effect of multiple functional groups, can effectively adsorb a complex heavy metal ion system in a water body, and is a heavy metal ion adsorbent which is high in adsorption efficiency, large in adsorption quantity and wide in applicable temperature range.

The present invention provides a novel resource-recycling technology for converting crustacean waste into violacein. Specifically, an engineered strain of Viola viride that efficiently metabolizes chitin and synthesizes violacein is disclosed. The engineered strain can be used for crustacean waste treatment and chitin resource conversion.

The invention discloses a preparation method of a PVA / chitin porous nanosheet layered film for salinity gradient power generation, and belongs to the technical field of bio-based material functionalization. The preparation method comprises the following operation steps: firstly, preparing a chitin porous nanosheet by utilizing an acid steam combined ultrasonic strategy; modifying the surface of the chitin porous nanosheet with rich positive charges through quaternization reaction, and performing suction filtration and drying to obtain a porous nanosheet layered film; and finally, circularly freezing, thawing and packaging the porous nanosheet layered film by using polyvinyl alcohol hydrogel to prepare the PVA / chitin porous nanosheet layered film. According to the PVA / chitin porous nanosheet layered film, by means of combination of chitin nanosheet layer gap channels and micro-nano pore channels in nanosheet surfaces, ion transmission paths are increased, ion transmission resistance is reduced, the microstructure of the layered film is effectively stabilized jointly by means of the interlayer weak force effect and the hydrogen bond bonding effect between polyvinyl alcohol molecules, and therefore the film has the advantages of being capable of being used for preparing the composite film. And the optimal salinity gradient power generation output power density reaches 7.33 W / m < 2 >.

The invention relates to the field of comprehensive utilization of biomass resources and preparation of high polymer materials, in particular to a green preparation method of high-deacetylation-degree chitin capable of recycling reaction waste liquid. The invention aims to solve the problems of serious environmental pollution, resource waste, low product purity and incomplete waste liquid recycling in chitin extraction. The method comprises the following steps: by taking crustacean biomass as a raw material, firstly carrying out high-voltage pulse electric field pretreatment to crush the tissue structure of the raw material and improve the reaction activity, and then heating and extracting in a choline chloride-lactic acid deep eutectic solvent system to realize efficient removal of proteins and inorganic salts in the raw material; according to the method, the chitin and the alkali liquor are subjected to a rapid deacetylation reaction by adopting microwave assistance and cooperating with the action of the phase transfer catalyst; according to the method, a waste liquid graded recycling system is adopted, hydrochloric acid in waste acid liquid generated by decalcification is recycled through electrodialysis, alkali liquid is recycled through ultrafiltration-nanofiltration double-membrane combination, deep eutectic solvent components are recycled through reduced pressure distillation, and recycling of acid, alkali and a solvent is achieved.

The invention relates to an application of carboxylated chitin or carboxylated chitosan in promoting insect-killing fungi to prevent and treat diseases and insect pests. Research finds that carboxylated chitin or carboxylated chitosan can significantly promote spore germination and hypha growth of insecticidal fungi, also can significantly enhance stress resistance of the insecticidal fungi, significantly enhance toxicity of the insecticidal fungi to pests, improve ultraviolet resistance and high temperature resistance of the insecticidal fungi, and improve the insecticidal activity of the insecticidal fungi. The invention also finds that the carboxylated chitin or chitosan can effectively inhibit the growth of Xanthomonas oryzae pv. Oryzae. Therefore, the carboxylated chitin or the carboxylated chitosan can be used for preparing a product for enhancing the insecticidal fungus effect and a product for preventing and treating the rice bacterial leaf blight, and the prevention and treatment of plant diseases and insect pests are realized. In addition, carboxylated chitin and carboxylated chitosan are derived from natural polymer materials, have the characteristics of safety, no toxicity, biodegradability, environmental friendliness and the like, are environment-friendly and economical, and have wide application prospects and market values.

The invention relates to an instant composition with a long-acting oral health-care function for dogs and cats as well as a preparation method and application of the instant composition, and belongs to the technical field of pet food and pet oral care. The composition comprises 20%-30% of whey protein powder, 10%-20% of hydrolyzed gelatin, 1%-3% of low-molecular-weight chitin saccharide gum, 1%-2% of modified cassava starch, 2%-5% of sodium alginate, 1%-3% of fructo-oligosaccharide, 0.03%-0.08% of lysozyme, 0.03%-0.08% of tea polyphenol, 0.05%-0.15% of zinc gluconate, 0.05%-0.1% of persimmon tannin, 3%-5% of tuna hydrolysate, 4%-6% of whole goat milk powder, 2%-4% of skim yoghurt powder and the balance water. The added active ingredients have long-acting slow-release and continuous effects in the formula, oral pathogenic bacteria can be effectively inhibited, dental plaque and dental calculus formation can be reduced, halitosis can be lastingly relieved, and the toothpaste has good market competitiveness.

The invention belongs to the technical field of agricultural fertilizers, and particularly relates to an amino acid water-soluble fertilizer containing chitosan oligosaccharide and salt-tolerant bacillus and a preparation method of the amino acid water-soluble fertilizer. According to the method, litopenaeus vannamei heads are used as raw materials, a refined solution is prepared through mixed acid pretreatment, double-enzyme fermentation and nanofiltration membrane desalination, and the refined solution is mixed with a salt-tolerant domesticated bacillus licheniformis / bacillus natto solution, citric acid-malic acid chelated medium elements and a protective agent. According to the method, by optimizing a pretreatment process and a chelating system, synergistic degradation of shrimp head protein and chitin is achieved, the prepared fertilizer has the compound functions of amino acid, chitosan oligosaccharide and a salt-tolerant microbial agent, the problems that a microbial agent in a traditional fertilizer is poor in salt tolerance and prone to precipitation are solved, and the method is suitable for facility agriculture and saline-alkali soil and has a wide application prospect. The crop stress resistance can be enhanced; the growth effect is improved.

The invention relates to the field of packaging materials, and particularly discloses a transparent high-barrier film and a preparation method thereof. The transparent high-barrier film comprises a matrix film and chitin nanofiber films positioned on two sides of the matrix film, the matrix film is prepared from the following raw materials in parts by weight: 50 to 80 parts of PBAT resin, 20 to 30 parts of PLA resin, 25 to 40 parts of modified poly epsilon-caprolactone, 0.5 to 1 part of compatilizer, 0.1 to 1 part of antioxidant, 15 to 25 parts of filler and 0.5 to 0.8 part of nucleating agent; the modified poly (epsilon-caprolactone) is prepared from the following raw materials in parts by weight: 80 to 100 parts of epsilon-caprolactone, 3 to 5 parts of cellulose nanofiber, 2.5 to 3 parts of halloysite aluminum-magnesium-loaded hydrotalcite, 100 to 120 parts of ionic liquid and 1 to 2 parts of catalyst. The transparent high-barrier film disclosed by the invention not only has excellent oxygen and water vapor barrier properties, mechanical properties and good biodegradability, but also has the light transmittance of 90% or above.

Insects with elevated levels of protein, chitin, elements, amino acids, vitamins, and / or sterols are disclosed. The insects have elevated levels due to a diet that includes mushroom biomass. The mushroom biomass includes any one or more of the mushroom fruitbody, the stem, and the mycelium. The enriched insects with biomolecules from mushroom biomass can be used as they are or as supplements to increase the levels of biomolecules in humans or other animals that may ingest the insects or a modified form of the insect.

The invention discloses biomass hard carbon and a preparation method and application thereof, and belongs to the technical field of carbon material preparation. The preparation method of the biomass hard carbon comprises the following steps: providing a dissolving solution of cellulose / chitin; adding the waste biomass into the dissolved solution, and stirring to obtain a solution dissolved with cellulose / chitin; directly adding a pickling solution for pickling to obtain regenerated cellulose / chitin; finally, pre-oxidation crosslinking and high-temperature carbonization are conducted, and the biomass hard carbon is prepared. According to the invention, the crystallinity of the regenerated cellulose is regulated and controlled, and a crystalline region and an amorphous region of the regenerated cellulose are reasonably matched, so that the derived biomass hard carbon has rich closed-cell structures and closed-cell rate and brings excellent electrochemical performance to a sodium-ion battery as a negative electrode material. The preparation method is mild in treatment condition and simple in treatment process, does not need to consume a large amount of acid and alkali, and has the advantages of high efficiency, economy and environmental friendliness.

The invention provides a compound microbial agent capable of resisting diseases and insect pests and a preparation method thereof, and the compound microbial agent consists of the following substances in parts by mass: 50-100 parts of bacillus subtilis, 40-80 parts of beauveria bassiana, 35-75 parts of trichoderma harzianum, 20-50 parts of modified straw powder, 10-15 parts of a humic acid-diatomite complex, 10-15 parts of trehalose, 5-10 parts of chitin powder and 5-10 parts of shellac. According to the invention, the modified straw powder and the humic acid-diatomite complex are adopted as a core carrier, so that the defects of poor adsorbability and nutrition deficiency of the traditional diatomite or synthetic carrier are overcome, and a high-porosity and nutrient-rich living environment is provided for bacillus subtilis, beauveria bassiana and trichoderma harzianum; compared with the prior art, the method provided by the invention has the advantages that the cost is low, the field planting rate and the field survival rate of viable bacteria in the microbial inoculum are remarkably improved, meanwhile, controllable coating is performed by utilizing an ethanol solution of shellac, a refractory synthetic polymer coating material is replaced, thalli are protected from environmental stress, and meanwhile, the risk of lasting residual pollution of a chemical coating material in soil is avoided.

The invention relates to technical development and application of new special medical food on the basis of a new food raw material framework, and belongs to the technical field of special medical purpose formula food (FSMP). According to the series of foods, new food raw materials (euglena, haematococcus pluvialis and ampelopsis grossedentata leaves) are taken as cores, medicinal and edible components (lotus leaves, haws and mulberry leaves) and marine active substances (fucoidan polysaccharides and chitin) are combined, functional factors are extracted through a prehydrolysis SBE technology and a biological enzymolysis technology, and the bioavailability is improved by adopting a nano-carrier technology. The product comprises a total nutrient base material (20-30% of protein and 15-25% of dietary fiber), functional components (10-15% of a new food raw material extract and 8-12% of a traditional Chinese medicine extract) and a metabolism regulator (conjugated linoleic acid and L-carnitine). Clinical tests show that after an obese patient takes 400 kcal of the replacement meal every day, the weight of the obese patient is reduced by 10.2 kg on average (4.8 kg of a control group) 12 weeks later, and the body fat rate is reduced by 16.3%. The product supports line iteration (such as powder, tablets and liquid food) through modular formula design, and fills the application blank of new food raw materials in special medical food.

The invention discloses a preparation method of Pickering emulsion, the prepared Pickering emulsion and application of the Pickering emulsion. Comprising the following steps: mixing an aqueous solution containing chitin nano-bundles with an oil phase to obtain Pickering emulsion; the aqueous solution containing the chitin nano-beams is obtained by the following steps: reacting raw materials containing water, p-toluenesulfonic acid and chitin, and separating to obtain the aqueous solution containing the chitin nano-beams. And the lower-layer product obtained in the preparation of the nano chitin is utilized, so that the full-component utilization of the chitin is realized. The prepared Pickering emulsion has no oil-water separation phenomenon within 7 days.

The invention discloses a full bio-based concrete admixture and a preparation method thereof, and belongs to the technical field of green building materials. The full bio-based concrete admixture is prepared from the following raw materials in parts by weight: 40 to 50 parts of lignin sulfonate, 10 to 15 parts of nano cellulose, 8 to 12 parts of tannic acid-iron ion chelate, 5 to 8 parts of nano silicon dioxide, 5 to 8 parts of pH sensitive chitin microgel, 10 to 12 parts of starch microcapsules and 3 to 5 parts of saponin. The invention also discloses a preparation method of the full bio-based concrete admixture. The full-bio-based concrete admixture can solve the problems of poor environmental protection property, poor compatibility and poor microcrack repairing performance of the existing admixture, and has wide application prospects.

The invention relates to preparation and application of a desoxyribonucleic acid and desoxyribonucleic acid derivative transdermal delivery system, and belongs to the technical field of biological medicine and skin care products. Desoxyribonucleic acid and derivatives thereof have the effects of resisting aging, repairing and the like, but are difficult to penetrate through a skin barrier due to large molecular weight and strong polarity. According to the invention, hydroxyethyl chitosan, desoxyribonucleic acid and derivatives thereof are combined through electrostatic interaction to form the nano-composite. The compound is small in particle size and can promote transdermal absorption through hair follicle or cuticle gaps. The preparation method is simple, does not need an auxiliary reagent, and comprises the step of mixing a hydroxyethyl chitosan solution and a deoxyribonucleic acid derivative solution. Experiments show that the compound significantly enhances the transdermal efficiency, and has the effects of promoting cell repair and proliferation and resisting inflammation. The composition can be used for skin care products, and is high in safety and wide in application prospect.

The invention belongs to the field of nano materials, and discloses a hydroxyapatite whisker / chitin whisker co-assembled liquid crystal as well as a preparation method and application thereof. The co-assembled liquid crystal is obtained by mixing hydroxyapatite whisker liquid crystal and chitin whisker liquid crystal which have the same electrical property, performing ultrasonic homogenization treatment and further stabilizing at room temperature. In a co-assembled liquid crystal system, the preparation and long-term stability of the co-assembled liquid crystal system are realized by two whiskers with the same electrical property based on a dual-drive principle of electrostatic repulsion and spatial orientation entropy; and the co-assembled liquid crystal simulates the liquid crystal phase and the ordered organic / inorganic phase composition in the living body in the nature in the organic / inorganic phase composition and the liquid crystal state. In addition, the adopted raw materials are wide in source and low in cost, the preparation process is simple, efficient and easy to implement, and no surfactant or dispersing agent is needed to stabilize the co-assembled liquid crystal system.

The invention discloses a ganoderma lucidum mutant strain as well as an open liquid fermentation method and application thereof. The Ganoderma lucidum mutant strain provided by the invention is Ganoderma lucidum MKX25023, and the registration number of the Ganoderma lucidum mutant strain in the China General Microbiological Culture Collection Center is CGMCC (China General Microbiological Culture Collection Center) No. 42078. The strain has no difference between biomass in an open culture environment and that in a sterilized culture environment, the stable yield of intracellular products (such as chitin) can be ensured, and when the strain is used as a chassis cell, the utilization value of the strain can be further expanded, and the industrialization practicability and economic value are remarkably improved.

The invention discloses a chitin-silicon-calcium-based modified material for rammed earth wall repair and a weather resistance evaluation method thereof, and belongs to the technical field of rammed earth wall repair, the chitin-silicon-calcium-based modified material comprises metakaolin, calcium oxide, quartz and chitin; the ratio of chitin to metakaolin to calcium oxide to quartz is 1: 1: 2: 4; the method comprises the following steps: pre-treating at the earlier stage of a test, evaluating freeze-thaw cycle performance, evaluating dry-wet cycle performance, evaluating salt corrosion resistance, analyzing association between a microstructure and a strength performance, and finally, revealing a material degradation rule and mechanism by combining scanning electron microscope observation, unconfined compressive strength test and crack rate analysis. When the freezing and thawing cycle number N is less than or equal to 5, the dry and wet cycle number I is less than or equal to 9, and the salt solution concentration c is less than or equal to 0.3 mol / L, the characteristics of freezing and thawing resistance, dry and wet resistance, salt erosion resistance and the like of the modified material are obviously improved; after the modified material is added, internal pores of plain soil can be filled, unconfined compressive strength of a soil body is enhanced, and long-term durability of the soil body is improved.

The invention discloses a chitin-based fluorescent film for Zr < 4 + > detection and a preparation method thereof. The material takes a chitin film as a substrate and is chemically modified in situ by a one-pot method. The method comprises the following steps: firstly, dissolving chitin in an alkali / urea system, preparing a chitin film through a tape casting method, and washing the film with deionized water until the pH value is neutral; then, a multi-component Mannich reaction is adopted, the chitin thin film obtained after hot pressing reacts with quercetin molecules and aldehyde compounds, and the modified covalent grafted chitin-based fluorescent thin film is obtained. Through introduction of quercetin molecules, the chitin film is endowed with fluorescence performance, and meanwhile, carbonyl and polyhydroxy structures of quercetin have the capability of easily forming strong complexes with metal ions, so that the modified chitin-based fluorescent film has the characteristic of quantitatively detecting the metal ions. The preparation method disclosed by the invention accords with green chemistry, is simple to operate and stable in performance, and can be used for rapidly and highly selectively detecting zirconium (IV) ions.

The invention relates to the technical field of continuous fiber reinforced composite materials, in particular to warp yarn for window decoration and a preparation process of the warp yarn. According to the invention, the problems of poor mechanical property and poor antibacterial property of traditional warp yarns for window decoration are solved. The preparation method comprises the following steps: mixing Bio-EG and Petrol-EG according to a ratio, reacting with terephthalic acid, precisely controlling the temperature, and adding a phosphorus stabilizer to obtain a high-performance polyester core material; chitin is modified by DMAA, nano silicon dioxide is modified by KH-560, and the chitin and the nano silicon dioxide are subjected to melt blending with a PLA matrix to form a reinforced sheath material; the sizing agent is composed of WPU, amino silicone oil microemulsion and other substances, and a protective film is formed on the surface of the fiber after the process is optimized; through core-sheath composite spinning and post-treatment processes such as segmented cooling, drafting and heat setting, the prepared warp yarn has high strength, high elongation, wear resistance, resilience and good antibacterial functions.

To provide a high-concentration dispersion of nanosized chitin having a uniform and fine fiber width.SOLUTION: A dispersion of nanosized chitin contains nanosized chitin with a fiber width of 20 nm or less in a concentration of 10 wt.%-30 wt.%.SELECTED DRAWING: Figure 1

The invention discloses allyl functionalized hyaluronic acid hydrogel as well as a preparation method and application thereof in electrolyte, and belongs to the technical field of hydrogel electrolyte. And dissolving the modified hyaluronic acid in a solvent, sequentially adding a cross-linking agent, the modified chitin nanofibers and an initiator, carrying out polymerization reaction at a preset temperature for a preset time to obtain hydrogel, and soaking the hydrogel in an electrolyte to obtain the modified hyaluronic acid hydrogel electrolyte. According to the scheme, the existing problems that a traditional hydrogel electrolyte is prone to icing at low temperature, the ionic conductivity is suddenly reduced, and the mechanical performance is degraded are solved, and the mechanical strength is enhanced by constructing an allyl functionalized hyaluronic acid three-dimensional chemical cross-linked network; modified chitin nanofibers are introduced to bind water molecules through hydrogen bonds to inhibit growth of ice crystals, graphene quantum dots are added to optimize an ion transmission path, and the hydrogel electrolyte which still keeps high ionic conductivity, excellent freezing resistance and high mechanical strength at the low temperature of-40 DEG C is prepared.

The invention discloses a bio-based repairable high-conductivity high-sensitivity sensing fiber as well as preparation and application thereof. According to the fiber, silk fibroin serves as a matrix, ChNCs serves as a dynamic cross-linking agent, an ion conductor and an electronic conductor are combined, and an ion-electron double-conduction network and a core-shell structure are constructed through microfluidic spinning. The fiber has high conductivity (gt, 5S / m), rapid self-healing (recovery of gt, 90% in 30 min), excellent mechanical strength (gt, 5MPa) and biodegradability, and is suitable for intelligent sensing fields such as wearable sensors, implantable medical equipment, human-computer interaction interfaces and the like.

The bioplastic additives and antimicrobial plastics include polyphenols and organic polymers. The polyphenol can be a phenol, a polyphenol, a bioflavonoid, a flavonoid, a tannin, a coumarin, a lagman, a quinone, a stilbene, or a qurcuminoidenin. The organic polymer may be chitosan, chitin, cellulose, and keratin. In an advantageous form, the polyphenol is hesperidin, and the organic biopolymer is chitosan.

The invention provides a nutrient solution formula. The nutrient solution formula comprises organic nitrogen, organic phosphorus, organic potassium, active calcium, quick-acting sulfur, magnesium, zinc, selenium, ferrous sulfate, organic silicon, expanded fertilizer, mineral source humic acid, chitin, live bacterial fertilizer, boric acid, manganese sulfate, zinc sulfate heptahydrate, ammonium molybdate, copper sulfate heptahydrate, disodium iron ethylenediaminetetraacetate, citric acid and water. According to the nutrient solution formula and the preparation system thereof provided by the invention, microelements are added into the formula, so that the nutrient requirements of vegetables during growth are met, the growth of the vegetables is promoted, and meanwhile, citric acid is added, so that the nutrient solution is stable in property, and the effectiveness of the nutrient solution can be kept in a relatively long period; the physiological acid-base reaction caused in the nutrient solution absorption process of the leaf vegetables can be kept stable, the activity of the root system is improved through high water-soluble phosphorus in the formula, the photosynthesis is improved through the magnesium element, long-acting biological sulfur is provided, and continuous energy supply is provided.

The invention provides a method for preparing high-activity chitosan oligosaccharide based on a microbial fermentation method. The method comprises the following steps: cooling a fermentation culture medium containing chitin powder, inoculating an aspergillus niger seed solution under stirring and ventilation, and fermenting for 24 hours; stopping stirring for 30 seconds, adding a soybean protein isolate solution, reducing the rotating speed to 180 rpm, and fermenting for 12 hours; continuously reducing the rotating speed to 150 rpm, adding a soybean protein isolate solution, and fermenting for 12-14 hours; and centrifuging the fermentation liquor, taking supernate, filtering, adding a xanthan gum solution, stirring, concentrating, drying in vacuum, and crushing, thereby obtaining the product. According to the method disclosed by the invention, the industrial pain points that oligosaccharide is easy to degrade and active sites are easy to oxidize in traditional fermentation are solved by virtue of segmented dynamic electrostatic combination and deep synergy of aspergillus niger directional fermentation enzyme production and soybean protein isolate, and the preparation of the high-activity chitosan oligosaccharide is realized.

This invention discloses a chitinase-producing Bacillus albus A01 and its application in the fermentation of shrimp and crab solid waste. The chitinase-producing Bacillus albus is named... Bacillus albus A01, with accession number CCTCC: M 20252571; the 16S rDNA gene sequence of this chitinase-producing Bacillus albus strain A01 is shown in SEQ ID NO.1. The fermentation agent of this strain can effectively degrade the chitin content in shrimp and crab solid waste, and improve the nutritional value of shrimp and crab fermented powder through solid-state fermentation. This invention solves the problem of unsatisfactory fermentation effects of existing feed fermentation strains, and has great application potential for improving the feed nutritional quality of shrimp and crab fermented powder and developing feed resources.