722 results about "Chitin" patented technology

A ternary deep eutectic solvent (TDES) system for a biomolecule (for example, chitin) recovery from a biomass (for example, seafood waste), and the method of preparation of ternary deep eutectic solvent (TDES) system, and a process to differentiate / extract / recover biomolecules (such as chitin) from a biomass (such as seafood waste).

The invention discloses an N-acetylglucosamine transferase AcetRS-R346D and an application thereof, and belongs to the technical field of functional enzymes. And the amino acid sequence of the N-acetylglucosamine transferase AcetRS-R346D is as shown in SEQ ID NO. 3. The invention further discloses the application of the N-acetylglucosamine transferase AcetRS-R346D in the preparation of the chitosan oligosaccharide. The N-acetylglucosamine transferase AcetRS-R346D disclosed by the invention is obtained by carrying out mutation modification on the N-acetylglucosamine transferase AcetRS, and can be used for producing the chitosan oligosaccharide with the polymerization degree of 4 to 7, especially (GlcNAc) 7. The product spectrum of the N-acetylglucosamine transferase is widened, and the N-acetylglucosamine transferase can be used for preparing high-polymerization-degree chitosan oligosaccharide and has a wide application prospect.

The invention belongs to the field of natural product extraction, and particularly relates to a method for extracting chitosan by using hermetia illucens in a high-valued manner. According to the method, the ionic liquid is introduced while the chitosan is extracted by adopting a deep eutectic solvent method, the deep eutectic solvent can form strong interaction with chitin molecules, the ionic liquid can further enhance specific recognition of the chitin molecules, and the ionic liquid and the deep eutectic solvent have a synergistic effect, so that solubility and recognizability of the chitin are improved; protein and salt are efficiently removed, meanwhile, glucosidic bonds of a chitin macromolecular main chain are prevented from being hydrolyzed and broken, and the extraction rate and purity of chitosan are remarkably improved. According to the method, the extraction condition is mild, the eutectic solvent can be recycled and reused, the extraction process flow of the chitosan is simplified, and the method is environment-friendly.

The invention belongs to the technical field of soil improvement, and relates to a soil conditioner for saline-alkali soil and a soil improvement method. The soil conditioner comprises vinegar residues, chitin, glucose, a composite biological preparation, a modified gel and a functional additive, the composite biological agent comprises bacillus subtilis powder, bacillus licheniformis powder, trichoderma spore powder, brown algae powder and light calcium carbonate; the functional auxiliary agent comprises modified bentonite, polyglutamic acid, pregelatinized starch and biochar potassium humate. Through the synergistic effect of multiple components and structural design optimization, systematic remediation of physical properties, chemical properties and biological activity of the saline-alkali soil is achieved. According to the technology, the limitation of single function of a traditional improvement measure is broken through, and the long-standing technical problems of return salt rebound, low fertility, microbial community imbalance and the like in saline-alkali soil treatment are effectively solved by constructing a multi-layer and multi-dimensional soil improvement system.

The invention relates to the technical field of lithium ion battery fire extinguishing agents, in particular to a lithium ion battery fire extinguishing agent and a preparation process. The preparation process comprises the following steps: S1, biomass pretreatment: mixing 40-50 parts of chitin powder with a 2wt% sodium hydroxide solution according to a mass volume ratio of 1: 10, stirring at 60 DEG C for 1-2 hours, and centrifuging and freeze-drying to obtain a chitin crude product; dispersing the chitin crude product in deionized water, and carrying out ultrasonic treatment to obtain a cellulose suspension with the solid content of 1%; s2, cellulose modification: adding 15-20 parts of povidone into the cellulose suspension, and mixing and stirring at 60 DEG C for 1 hour to obtain a modified cellulose suspension; s3, introducing nano silicon dioxide; s4, preparing modified gel; and S5, cross-linking and drying.

The invention belongs to the technical field of biomedical materials, and particularly relates to a multi-layer multifunctional hydrogel microsphere for diabetic wounds as well as a preparation method and application of the multi-layer multifunctional hydrogel microsphere. The preparation method comprises the following steps: firstly, synthesizing a chitosan derivative with proper amino and aldehyde group contents, oxidized macromolecules and modified chitin; then preparing photocuring hydrogel microspheres by utilizing a microfluidic technology, and sequentially immersing the photocuring hydrogel microspheres into an oxidized polymer solution, a modified chitin solution, a tannic acid solution and a metal salt solution, so as to construct the hydrogel microspheres with a multifunctional layer structure. The invention further provides specific application intervals of parameters such as solvents used by various solutions, concentration ranges and soaking time. The preparation method provided by the invention has expansibility and universality, various types of microspheres can be constructed for sequential release of bioactive substances, and the requirements of tissue repair are met.

The invention relates to chitin-based acrylic resin and a preparation method thereof, and belongs to the technical field of intersection of high value-added utilization of biomass resources and acrylic resin. The chitin-based acrylic resin is prepared from the following components in parts by weight: 30 to 50 parts of acrylic modified chitin, 20 to 30 parts of acrylic monomer, 10 to 20 parts of polyaldehyde monomer, 5 to 10 parts of polybasic organic amine, 1 to 5 parts of emulsifier, 0.1 to 0.5 part of initiator, 0.2 to 0.4 part of defoaming agent and 80 to 120 parts of deionized water. According to the method, forestry and agricultural residues are used as raw materials, chitin with small molecular chains is obtained through deacetylation treatment, meanwhile, a large number of primary amino groups with high chemical reactivity are exposed on the surfaces of the molecular chains of the chitin, and 3-formyl-2-butenoic acid is further utilized to react with the chitin, so that the yield of the chitin is increased. Acrylic acid groups are successfully grafted to chitin molecular chains to be subjected to polymerization reaction with acrylic acid monomers to form macromolecule resin, meanwhile, the macromolecule resin is subjected to cross reaction with polyaldehyde and polybasic organic amine to generate net-shaped crosslinking, and the strength of the acrylic resin after film forming is improved.

The invention belongs to the technical field of green processing of biomass materials, and particularly relates to a green preparation process of high-deacetylation-degree chitin. The preparation method aims at the problem of high energy consumption of existing high-deacetylation-degree chitin preparation. According to the method, raw materials are pretreated by utilizing a compound enzyme system under a low-temperature condition, chitin crystal lattices are preliminarily dissociated by combining a deep eutectic solvent at a medium temperature, high deacetylation conversion is realized through short-time dissolution of an ionic liquid, and meanwhile, a membrane separation-electrodialysis-gradient freezing crystallization three-stage recycling technology is matched, so that recycling of the solvent is realized; the method greatly reduces the reaction temperature and energy consumption, avoids the use of corrosive reagents, and has the advantages of environmental protection, low energy consumption, high deacetylation degree of the product, excellent quality and the like.

The invention discloses a preparation method and application of crab shell chitin without chemical decolorization, and the preparation method comprises the following steps: crushing the crab shell of Eriocheir sinensis to obtain crab shell powder; dissolving crab shell powder in distilled water according to a ratio of 1: 10-1: 30 (w / v) to obtain a crab shell powder solution; treating the crab shell powder solution by using plasma jet (PJ), washing to be neutral, and drying to obtain plasma pretreated crab shell powder; dissolving the crab shell powder pretreated by the plasma into 0.1-3M hydrochloric acid (HCl) solution according to the ratio of (1: 10)-(1: 30) (w / v), and carrying out demineralization treatment; washing the demineralized solid matter to be neutral, performing deproteinization treatment by using 0.1-3M sodium hydroxide (NaOH) solution, washing to be neutral, and drying to obtain white solid crab shell chitin. In the extraction process of the crab shell chitin, a strong oxidant (such as H2O2) is not required to be used for decoloring, the color is close to that of commercial chitin, and the process is simple, green and environment-friendly; meanwhile, the production cost is reduced.

The invention relates to the field of water treatment, in particular to a composite material with polyfunctional groups and a preparation method and application thereof.The composite material with the polyfunctional groups contains at least two groups including carboxyl, amino, amido and hydroxyl and at least one group including sulfo and nitro; the composite material contains a cross-linked compound of a polymer I and a polymer II, and the polymer I is a carbon chain polymer and contains at least one group including a sulfonic acid group and a nitro group; and the polymer II is at least one of chitosan and a derivative thereof, and chitin and a derivative thereof. The composite material with multiple functional groups is preferably a composite fiber, has a synergistic adsorption effect of multiple functional groups, can effectively adsorb a complex heavy metal ion system in a water body, and is a heavy metal ion adsorbent which is high in adsorption efficiency, large in adsorption quantity and wide in applicable temperature range.

The invention discloses bio-based polyester and a preparation method thereof, and belongs to the technical field of biomass resource utilization and bio-based polyester, and the bio-based polyester comprises the following components: deacetylated chitosan, aldehyde modified lignin, polybasic organic amine, polybasic aldehyde monomer, a catalyst, 1, 4-dioxane, methanol and deionized water. The method comprises the following steps: extracting chitosan from shrimp / crab shells, and exposing primary amino groups through alkali treatment; the method comprises the following steps: extracting lignin from coffee grounds, and grafting an aldehyde group with a polyaldehyde monomer; the aldehyde modified lignin and chitosan are subjected to Michael addition reaction and then are crosslinked with polyaldehyde monomers and polybasic organic amine to form a net structure, so that the film forming strength is improved. The environment burden caused by the coffee grounds and shrimp shells or crab shells is reduced, and meanwhile, a low-cost and environment-friendly bio-based polyester is provided for the field of coatings.

The multifunctional environment-friendly and energy-saving flowerpot comprises a pot body and a tray, a boss is arranged on a bottom layer plate of an inner cavity of the pot body, and a drainage hole is formed in the middle of the boss. One end of the honeycomb ceramic tube is communicated with the inner cavity of the pot body, the other end of the honeycomb ceramic tube penetrates through the drain hole and is flush with the lower surface of the bottom plate of the pot body or is sleeved on the boss, the thickness of the wall of the honeycomb ceramic tube is 30-40% of the diameter, the porosity of the honeycomb ceramic tube is 40-70%, and the honeycomb ceramic tube contains pelelith and chitin. According to the utility model, frequent watering is not needed, soil can always keep moist humidity for growth of the plant bonsai for a long time, water is not accumulated, and hot air generated by water evaporation can be timely discharged when the bonsai is heated at high temperature, so that the bonsai cannot be dried up and burnt when the room temperature rises; therefore, the potted landscape can grow normally for a long time, the pot body is bactericidal and antibacterial, water molecules are refined to be conveniently absorbed by a root system, and the functions of constant temperature, heat preservation, ventilation and the like are achieved.

The present invention provides a novel resource-recycling technology for converting crustacean waste into violacein. Specifically, an engineered strain of Viola viride that efficiently metabolizes chitin and synthesizes violacein is disclosed. The engineered strain can be used for crustacean waste treatment and chitin resource conversion.

The invention discloses a preparation method of a PVA / chitin porous nanosheet layered film for salinity gradient power generation, and belongs to the technical field of bio-based material functionalization. The preparation method comprises the following operation steps: firstly, preparing a chitin porous nanosheet by utilizing an acid steam combined ultrasonic strategy; modifying the surface of the chitin porous nanosheet with rich positive charges through quaternization reaction, and performing suction filtration and drying to obtain a porous nanosheet layered film; and finally, circularly freezing, thawing and packaging the porous nanosheet layered film by using polyvinyl alcohol hydrogel to prepare the PVA / chitin porous nanosheet layered film. According to the PVA / chitin porous nanosheet layered film, by means of combination of chitin nanosheet layer gap channels and micro-nano pore channels in nanosheet surfaces, ion transmission paths are increased, ion transmission resistance is reduced, the microstructure of the layered film is effectively stabilized jointly by means of the interlayer weak force effect and the hydrogen bond bonding effect between polyvinyl alcohol molecules, and therefore the film has the advantages of being capable of being used for preparing the composite film. And the optimal salinity gradient power generation output power density reaches 7.33 W / m < 2 >.

The invention discloses a magnetic immobilized enzyme and a preparation method and application thereof in preparation of chitosan oligosaccharide, the immobilized enzyme comprises a carrier and an enzyme loaded on the carrier, the carrier is a magnetic nanoparticle modified double-hole silicon dioxide nanoparticle, the double-hole silicon dioxide nanoparticle simultaneously contains a plurality of macropores and a plurality of micropores, and the macropores and the micropores are uniformly distributed on the carrier. The aperture of the large hole is 20-40nm, the aperture of the small hole is 2-5nm, and the magnetic immobilized enzyme has strong magnetism, is more rapid and convenient to recover, has good reusability, is simple and convenient to operate, has a wide application prospect, and is suitable for industrial preparation of chitosan oligosaccharide.

The invention discloses polysaccharide fluid hydrogel for digestive tract submucous injection and a preparation method of the polysaccharide fluid hydrogel, and belongs to the field of medical biological materials. According to the invention, carboxylated chitin or a carboxylated chitosan derivative with a mass percentage of 0.1-6%, or hydroxyalkylated chitin or a hydroxyalkylated chitosan derivative and collagen or gelatin are subjected to micro-crosslinking to form a low-crosslinking-degree supramolecular derivative as a solute; the polysaccharide fluid hydrogel is formed by taking water, normal saline, a physiological equilibrium solution, a phosphate buffer solution or a Green's solution as a solvent. By controlling the molar ratio of the raw materials to the cross-linking agent and performing micro-crosslinking with a small amount of the cross-linking agent, the prepared low-crosslinking-degree supramolecular derivative can form hydrogel in an aqueous solvent, has shear thinning and self-repairing characteristics, has certain viscosity, is easy to push and inject, and can be applied to the field of water treatment. The lifting height of the mucous membrane can be effectively kept, the height keeping time can be effectively prolonged, the mucous membrane is not prone to overflowing under the condition that the mucous membrane is damaged, an endoscope injection device is not blocked, and the material is an ideal material for forming a safe liquid pad through digestive tract submucous injection.

The invention relates to the field of comprehensive utilization of biomass resources and preparation of high polymer materials, in particular to a green preparation method of high-deacetylation-degree chitin capable of recycling reaction waste liquid. The invention aims to solve the problems of serious environmental pollution, resource waste, low product purity and incomplete waste liquid recycling in chitin extraction. The method comprises the following steps: by taking crustacean biomass as a raw material, firstly carrying out high-voltage pulse electric field pretreatment to crush the tissue structure of the raw material and improve the reaction activity, and then heating and extracting in a choline chloride-lactic acid deep eutectic solvent system to realize efficient removal of proteins and inorganic salts in the raw material; according to the method, the chitin and the alkali liquor are subjected to a rapid deacetylation reaction by adopting microwave assistance and cooperating with the action of the phase transfer catalyst; according to the method, a waste liquid graded recycling system is adopted, hydrochloric acid in waste acid liquid generated by decalcification is recycled through electrodialysis, alkali liquid is recycled through ultrafiltration-nanofiltration double-membrane combination, deep eutectic solvent components are recycled through reduced pressure distillation, and recycling of acid, alkali and a solvent is achieved.

The invention relates to an application of carboxylated chitin or carboxylated chitosan in promoting insect-killing fungi to prevent and treat diseases and insect pests. Research finds that carboxylated chitin or carboxylated chitosan can significantly promote spore germination and hypha growth of insecticidal fungi, also can significantly enhance stress resistance of the insecticidal fungi, significantly enhance toxicity of the insecticidal fungi to pests, improve ultraviolet resistance and high temperature resistance of the insecticidal fungi, and improve the insecticidal activity of the insecticidal fungi. The invention also finds that the carboxylated chitin or chitosan can effectively inhibit the growth of Xanthomonas oryzae pv. Oryzae. Therefore, the carboxylated chitin or the carboxylated chitosan can be used for preparing a product for enhancing the insecticidal fungus effect and a product for preventing and treating the rice bacterial leaf blight, and the prevention and treatment of plant diseases and insect pests are realized. In addition, carboxylated chitin and carboxylated chitosan are derived from natural polymer materials, have the characteristics of safety, no toxicity, biodegradability, environmental friendliness and the like, are environment-friendly and economical, and have wide application prospects and market values.

The invention provides a cordyceps militaris mycelium and a preparation method and application thereof, and relates to the technical field of biological materials. According to the invention, chitinase is added into the cordyceps militaris mycelium material to carry out enzymolysis on chitin to obtain chitooligosaccharide and N-acetylglucosamine, so that beta-glucan in a mycelium framework is dissolved out, and the grafting efficiency of the cordyceps militaris mycelium material is improved; furthermore, the antibacterial effect of the antibacterial material is improved by carrying out grafting reaction on the cordyceps militaris mycelium material and an exogenous monomer antibacterial peptide which has antibacterial performance and contains carboxyl groups.

The invention provides a soil stabilizer and a preparation method thereof, and belongs to the technical field of soil stabilizers. The soil stabilizer comprises humic acid, chitin, polyacrylamide, diatomite, iron powder, compound bacteria and a water-retaining agent. According to the soil stabilizer disclosed by the invention, the humic acid and the chitin are added, so that rich nutrient elements are provided for soil, and the soil structure is improved; secondly, the diatomite is modified, and the water-retaining agent is added, so that the water-retaining capacity of the soil is improved; the organic environment of the soil is improved by adding the iron powder and the compound bacteria. The soil stabilizer disclosed by the invention not only can improve the soil structure of a farmland, but also can enhance the fertility of the farmland, increase the content of organic matters in the soil and the number of microorganisms, improve the microorganism living environment of the soil, and further improve the quality and the number of crops in the farmland.

The invention relates to an instant composition with a long-acting oral health-care function for dogs and cats as well as a preparation method and application of the instant composition, and belongs to the technical field of pet food and pet oral care. The composition comprises 20%-30% of whey protein powder, 10%-20% of hydrolyzed gelatin, 1%-3% of low-molecular-weight chitin saccharide gum, 1%-2% of modified cassava starch, 2%-5% of sodium alginate, 1%-3% of fructo-oligosaccharide, 0.03%-0.08% of lysozyme, 0.03%-0.08% of tea polyphenol, 0.05%-0.15% of zinc gluconate, 0.05%-0.1% of persimmon tannin, 3%-5% of tuna hydrolysate, 4%-6% of whole goat milk powder, 2%-4% of skim yoghurt powder and the balance water. The added active ingredients have long-acting slow-release and continuous effects in the formula, oral pathogenic bacteria can be effectively inhibited, dental plaque and dental calculus formation can be reduced, halitosis can be lastingly relieved, and the toothpaste has good market competitiveness.

The invention belongs to the technical field of agricultural fertilizers, and particularly relates to an amino acid water-soluble fertilizer containing chitosan oligosaccharide and salt-tolerant bacillus and a preparation method of the amino acid water-soluble fertilizer. According to the method, litopenaeus vannamei heads are used as raw materials, a refined solution is prepared through mixed acid pretreatment, double-enzyme fermentation and nanofiltration membrane desalination, and the refined solution is mixed with a salt-tolerant domesticated bacillus licheniformis / bacillus natto solution, citric acid-malic acid chelated medium elements and a protective agent. According to the method, by optimizing a pretreatment process and a chelating system, synergistic degradation of shrimp head protein and chitin is achieved, the prepared fertilizer has the compound functions of amino acid, chitosan oligosaccharide and a salt-tolerant microbial agent, the problems that a microbial agent in a traditional fertilizer is poor in salt tolerance and prone to precipitation are solved, and the method is suitable for facility agriculture and saline-alkali soil and has a wide application prospect. The crop stress resistance can be enhanced; the growth effect is improved.

The invention discloses hydrogel for preventing postoperative tissue adhesion and a preparation method thereof, and belongs to the field of medical biological materials. The hydrogel is formed by taking a carboxylated chitin / chitosan derivative with the percentage concentration of 0.5-7% or a low-crosslinking-degree supramolecular derivative of a hydroxyalkylated chitin / chitosan derivative and collagen or gelatin as a solute and water, normal saline, a physiological equilibrium solution, a phosphate buffer solution or a Green's solution as a solvent, the crosslinking degree of the low-crosslinking-degree supramolecular crosslinking derivative is 0.1 to 6 mol percent. The molar ratio of the raw materials to the cross-linking agent is controlled, a small amount of cross-linking agent is used for micro-crosslinking, the prepared low-crosslinking-degree supramolecular derivative can form hydrogel in an aqueous solvent, and the hydrogel has adhesiveness and self-repairing performance, can effectively prolong the residence time and physical barrier time of the hydrogel on the surface of an operative wound, and can be used for preparing the hydrogel. Therefore, the effect of preventing postoperative combined adhesion of the hydrogel is improved, and the hydrogel is an ideal hydrogel material for preventing postoperative abdominal cavity and uterine cavity tissue adhesion.

The invention discloses a high-efficiency green extraction method of semen plantaginis chitin, and belongs to the technical field of natural product extraction. The method comprises the steps of raw material pretreatment, ultrasonic-assisted enzymolysis, enzyme deactivation, centrifugal separation, ultrafiltration concentration, alcohol precipitation and drying. The core innovation lies in that an ultrasonic-assisted composite enzymolysis synergistic technology is adopted, the dissolution rate of the polysaccharide is remarkably increased, the enzyme activity is protected, and finally the high-purity polysaccharide with the molecular weight of 9.2 kDa and the extraction rate of 13.82% is obtained. Compared with a traditional method, intermittent ultrasound and low-temperature enzymolysis are cooperated, so that polysaccharide chain breakage is effectively reduced; the compound enzyme degrades cell wall components in a targeted manner and breaks through the efficiency bottleneck of a single enzyme; toxic solvents are avoided in the whole process, and the process is environment-friendly. The data of the embodiment shows that the extraction rate is increased by 30% or above compared with that of a single enzyme method, the molecular weight distribution of the product is uniform, and the method is suitable for large-scale production of functional polysaccharides in the fields of food, medicine and the like.

The invention relates to the field of packaging materials, and particularly discloses a transparent high-barrier film and a preparation method thereof. The transparent high-barrier film comprises a matrix film and chitin nanofiber films positioned on two sides of the matrix film, the matrix film is prepared from the following raw materials in parts by weight: 50 to 80 parts of PBAT resin, 20 to 30 parts of PLA resin, 25 to 40 parts of modified poly epsilon-caprolactone, 0.5 to 1 part of compatilizer, 0.1 to 1 part of antioxidant, 15 to 25 parts of filler and 0.5 to 0.8 part of nucleating agent; the modified poly (epsilon-caprolactone) is prepared from the following raw materials in parts by weight: 80 to 100 parts of epsilon-caprolactone, 3 to 5 parts of cellulose nanofiber, 2.5 to 3 parts of halloysite aluminum-magnesium-loaded hydrotalcite, 100 to 120 parts of ionic liquid and 1 to 2 parts of catalyst. The transparent high-barrier film disclosed by the invention not only has excellent oxygen and water vapor barrier properties, mechanical properties and good biodegradability, but also has the light transmittance of 90% or above.

The invention relates to the technical field of crop cultivation. In particular to a rice leaf fertilizer and a fertilizing method and application thereof. The rice leaf fertilizer is prepared from the following components in percentage by weight: 10 to 20 percent of chitin derivative, 5 to 15 percent of compound amino acid, 5 to 10 percent of composted livestock and poultry manure, 5 to 15 percent of fulvic acid, 1 to 3 percent of zinc sulfate, 0.5 to 2 percent of borax, 0.2 to 0.5 percent of potassium silicate, 0.1 to 0.5 percent of polyether modified siloxane, 0.05 to 0.1 percent of rhamnolipid, 0.1 to 0.15 percent of betaine, 1 to 2 percent of sodium alginate and the balance of water. By applying the foliar fertilizer in drought and salt stress environments, the content of beta-glucan in rice is increased, the rice foliar fertilizer disclosed by the invention not only can be popularized for planting rice in northern drought regions in China, but also the planted rice has higher beta-glucan content, and has a health assisting effect on diabetics with limited starch consumption.

Insects with elevated levels of protein, chitin, elements, amino acids, vitamins, and / or sterols are disclosed. The insects have elevated levels due to a diet that includes mushroom biomass. The mushroom biomass includes any one or more of the mushroom fruitbody, the stem, and the mycelium. The enriched insects with biomolecules from mushroom biomass can be used as they are or as supplements to increase the levels of biomolecules in humans or other animals that may ingest the insects or a modified form of the insect.

The invention discloses biomass hard carbon and a preparation method and application thereof, and belongs to the technical field of carbon material preparation. The preparation method of the biomass hard carbon comprises the following steps: providing a dissolving solution of cellulose / chitin; adding the waste biomass into the dissolved solution, and stirring to obtain a solution dissolved with cellulose / chitin; directly adding a pickling solution for pickling to obtain regenerated cellulose / chitin; finally, pre-oxidation crosslinking and high-temperature carbonization are conducted, and the biomass hard carbon is prepared. According to the invention, the crystallinity of the regenerated cellulose is regulated and controlled, and a crystalline region and an amorphous region of the regenerated cellulose are reasonably matched, so that the derived biomass hard carbon has rich closed-cell structures and closed-cell rate and brings excellent electrochemical performance to a sodium-ion battery as a negative electrode material. The preparation method is mild in treatment condition and simple in treatment process, does not need to consume a large amount of acid and alkali, and has the advantages of high efficiency, economy and environmental friendliness.

The invention provides a compound microbial agent capable of resisting diseases and insect pests and a preparation method thereof, and the compound microbial agent consists of the following substances in parts by mass: 50-100 parts of bacillus subtilis, 40-80 parts of beauveria bassiana, 35-75 parts of trichoderma harzianum, 20-50 parts of modified straw powder, 10-15 parts of a humic acid-diatomite complex, 10-15 parts of trehalose, 5-10 parts of chitin powder and 5-10 parts of shellac. According to the invention, the modified straw powder and the humic acid-diatomite complex are adopted as a core carrier, so that the defects of poor adsorbability and nutrition deficiency of the traditional diatomite or synthetic carrier are overcome, and a high-porosity and nutrient-rich living environment is provided for bacillus subtilis, beauveria bassiana and trichoderma harzianum; compared with the prior art, the method provided by the invention has the advantages that the cost is low, the field planting rate and the field survival rate of viable bacteria in the microbial inoculum are remarkably improved, meanwhile, controllable coating is performed by utilizing an ethanol solution of shellac, a refractory synthetic polymer coating material is replaced, thalli are protected from environmental stress, and meanwhile, the risk of lasting residual pollution of a chemical coating material in soil is avoided.

The invention discloses a method for extracting chitin in shrimp shells by a ball-milling-assisted liquid acid room-temperature one-step method, which comprises the following steps: 1) washing the shrimp shells in boiling water, then taking out, drying to constant weight, crushing and sieving to obtain shrimp shell powder; 2) mixing the shrimp shell powder with 15 wt% HCl, and carrying out ball milling in a zirconium dioxide ball milling tank for 12-16 hours; and (3) after the ball milling is completed, washing the mixed milling product by using deionized water, and drying the solid obtained by washing to constant weight, so as to obtain the chitin product. According to the method, an alkaline reagent is not used, meanwhile, the acid consumption is greatly reduced, additional heating is not needed, and the technological process is shortened. The method has the advantages of simple process, mild conditions, environment friendliness, less waste liquid and the like, and is a green and environment-friendly process system.