Microbial agent for repairing petroleum-polluted saline alkali soil and preparation method thereof

Abstract

The invention relates to a microbial agent for repairing petroleum-polluted saline alkali soil and a preparation method thereof, and belongs to the technical field of resource environment. According to a principle of microbial ecology, microbial community is extracted and screened from soils with high salt and alkali and high petroleum pollution to prepare the microbial agent, wherein obligate petroleum degrading bacteria can be sphingolipid bacteria, comamonas, chitin autophagy bacteria and bacillus. According to culture medium and soil environment experiments, the microbial agent can guarantee that the extracted microorganism can be survived in the soils with the salt content of 5,000mg / kg, and meanwhile, the degradation capability to the polluted soils with the petroleum content of 10-50g / kg can reach 80%. The microbial agent has the advantages of simple process, rich microorganisms, obvious dominant bacteria, good stability and capability of facilitating obligate petroleum degrading bacteria to adapt to a new pollution environment, increasing and improving the micro-ecological environment quality of the soils and avoiding secondary pollution to the environment, and can obviously improve the degradation efficiency of petroleum hydrocarbon in the saline alkali soil.

Description

technical field [0001] The invention relates to the remediation of oil pollution in soil, in particular to a microbial agent for remediation of oil-polluted saline-alkali soil and its preparation. Background technique [0002] With the rapid development of the petroleum industry, the amount of oil extraction and use is increasing year by year. Therefore, the pollution to the soil environment caused by oil field mining areas, long-distance transportation and use of oil is becoming more and more serious. [0003] When petroleum pollutants enter the soil, it will lead to changes in the physical and chemical properties of the soil, such as the reduction of soil permeability and the reduction of effective water conduction channels in the soil, which will cause obstacles to plant root respiration and water and nutrient intake from the soil, thereby affecting plant growth and development. At the same time, due to the influence of pollutants, the carbon-nitrogen ratio of the soil c...

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Problems solved by technology

[0055] The purpose of the present invention is to solve the pollution problem of petroleum and saline-alkali dual stress urgently for oil-polluted saline-alkali soil, and to provide a kind of microbial agent and preparation method for repairing oil-polluted saline-alkali soil. The extracted microorganisms can survive in soil with a high salinity of 5000mg / kg, and have a degradability of up to 80% for contaminated soil with a petroleum content of 10000-50000mg / kg

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