44 results about "Comamonas" patented technology

The invention discloses a highly effective degradation strain JN6 for petroleum hydrocarbons in oily sludge and application thereof, which belong to the technical field of bioremediation. The degradation strain JN6 is preserved in the China General Microbiological Culture Collection Center, the preservation number is CGMCC NO. 14977, and the classification number is Comamonas sp. The strain JN6 with a petroleum hydrocarbon degradation function which is disclosed by the invention is applied to treatment of oily sludge by degrading petroleum hydrocarbons, the degradation rate of the single strain of bacteria on total petroleum hydrocarbon (TPH) in oily sludge is 62.16 percent within 30 days, and the optimum degradation conditions of the bacteria on petroleum pollutants are 15 DEGC to 40 DEGC and the pH value of 7 to 11. The biological method is used in oily sludge remediation technology, and has the advantages of high effectiveness, low cost, environment-friendliness and the like.

The invention relates to a Comamonas testosteroni engineering bacterium. Directional regulation is adjusted and controlled to Comamonas testosterone through a DNA recombination technology, so as to produce Comamonas testosteroni AMCa 3 (CGMCC No.2364) having the capability of efficiently degrading steroid compounds. The engineering bacterium can effectively degrade testosterone, cholesterol and other steroid compounds, has good degradation effect to soils and livestock-poultry excreta seriously polluted by steroid compounds, and can be used for the treatment of livestock-poultry excreta, producing low-cholesterol food in food industry, and transforming steroid substances in the production of feed additives and dairy products for producing pollution-free food. The engineering bacterium has important significance in environmental remediation and agricultural application.

Recalcitrant chemical oxygen demand (COD) of a liquid is reduced in a water treatment system. The method includes pretreating the liquid in a pretreatment unit to remove indigenous bacteria or microbes to a population level below which the indigenous organisms can interfere with the screened and externally introduced microorganisms. The liquid is then provided to a reactor that has a filter bed formed with a carrier material. Special microbes are screened and used to colonize the carrier material to remove recalcitrant COD. A biofilm is cultured on the surface of the carrier material to immobilize the screened microbes in the reactor. The method further includes percolating the liquid from the pretreatment unit through the filter bed colonized with the screened microbes to degrade at least part of the recalcitrant COD under aerobic conditions. In one embodiment, the filter is formed with biological granular activated carbon (GAC) as the carrier material and the screened microbes comprise at least one microbial species selected from the group consisting of Bacillus, Comamonas, Arthrobacter, Micrococcus, Pseudomonas, Pediococcus, Achromobacter, Flavobacterium, Mycobacterium, Rhodanobacter, Stenotrophomonas and Yeast.

The invention relates to a constitutive expression genetically engineered bacterium Escherichia coli CICC 11032S for producing L-alanine. The host is escherichia coli CICC 11022S (same as CGMCC No. 5450) producing L-aspartase, and contains L-aspartate beta-decarboxylase gene of comamonas testosterone CICC 11023S (same as CGMCC No. 6083). By utilizing the constructed engineered bacterium for whole-cell high-density catalysis, fumaric acid and ammonia water can be efficiently conversed for producing L-alanine, the output is 112.7 g/L, the production rate is 12.5 g/(L*h), and the conversion rate is 93.9%. The substrates are cheap, the production process is simple, by-products are less, the production cost is effectively reduced, and extremely good application value is provided.

The invention relates to burkholderia caribensis and comamonas for efficiently degrading atrazine. A mixed fermental culture medium formula and a culture method for high-density cell culture are obtained through response surface optimization design, and specially, a mixed bacterium for efficiently degrading atrazine and a fermental culture method are provided. According to the mixed bacterium, the burkholderia caribensis and the comamonas for efficiently degrading atrazine are utilized; a formula of a fermental culture medium for high-density cell culture is obtained through response surface optimization design; the formula comprises 6.72g of cane sugar, 6.75g of glucose, 6.34g of bean pulp, 0.6g of dipotassium phosphate, 2.5g of monopotassium phosphate, 0.3g of magnesium sulfate heptahydrate, 0.4g of sodium chloride, 3g of yeast powder and 1L of distilled water; the pH is 7.0-7.5. The cultured mixed bacterial liquid is adopted for soil remediation tests, and 200mg/L of atrazine can be completely degraded within 7 days. A high-density fermental culture formula and method are provided for development of the mixed bacteria.

The invention discloses an iron-reducing comamonas and an application thereof. The inventor screens the iron-reducing comamonas with the humus reduction activity and the iron reduction activity by a great deal of experiments. The bacteria can reduce the ferric iron such as ironic citrate, iron hydroxide, ferrihydrite and goethite; and reduce the humus and the humic model AQDS (anthraquinone-2, 6-disulfonic acid). The iron-reducing comamonas is the facultative anaerobe, has wide electro donor spectrum. The electro donors which can be oxidized by the bacteria in the anaerobic condition are glycerine, glucose and sucrose.

Provided is a polychlorinated biphenyl-decomposing composition obtained according to a method comprising respectively culturing at least one main microbial strain belonging to Comamonas species and having biphenyl dioxygenase, and at least one complementary microbial strain selected from the group consisting of Pseudomonas species, Achromobacter species, Rhodococcus species and Stenotrophomonas species and having biphenyl dioxygenase, and mixing at least two types of microbial cells recovered from each of the culture media. A composition containing these compounded microorganisms is useful for efficiently decomposing or detoxifying comparatively low concentrations of PCBs present in large amounts in waste products contaminated with polychlorinated biphenyls.

The invention provides comamonas capable of effectively degrading picolinic acid and an application of the comamonas, and belongs to the technical field of bioengineering. According to the invention, a picolinic acid degrading bacterium, namely comamonas, is separated and screened out, and the comamonas is classified and named as comamonas sp., which is preserved in China General Microbiological Culture Collection Center with preservation number of CGMCC NO.10700. The comamonas disclosed by the invention has the advantages that the comamonas is capable of effectively degrading the picolinic acid within a relatively wide pH value range, in particular under the circumstance of relatively high alkalinity; and the comamonas, as a biological enhancing preparation, can be applied to aerobic biological treatment of picolinic acid wastewater in weak base and weak acid environments; therefore, the comamonas has a broad application prospect.

The invention belongs to the technical field of sewage treatment, and discloses a compound microorganism water purifying agent and an application thereof. The compound microorganism water purifying agent comprises a microorganism and a carrier, the microorganism comprises clostridium, saccharomycetes, micrococcus, acidobacteria, bacteroid, rhizobia, burkholderia cenocepacia, thiobacillus, opitutae, hydrogenophilales, lysobacter, brevundimonas, xanthomonas, comamonas, rhodocyclus and rhodospirillum, and the microorganism is fixed onto the carrier. The compound microorganism water purifying agent has high activity and is high in treatment efficiency, long-time domestication and selection is omitted, a short stable period is achieved in a treatment system, strains in the compound microorganism water purifying agent are cooperatively interact, utilization and metabolic decomposition of nutrient substances in water by the microorganism are facilitated, and COD (chemical oxygen demand) and ammonia nitrogen are efficiently reduced.

The invention provides aquatic comamonas and a culture method and application thereof. The aquatic comamonas is KY-02, and the preservation number is CGMCC No.11866. The aquatic comamonas KY-02 can degrade cellulose with the content of 1500 mg/L in sewage, so that the COD of sewage is lowered, the degradation onset time is short, and the degradation rate can reach about 15% on the second day. Compared with physical and chemical methods, the aquatic comamonas has the advantages of being low in cost, free of secondary pollution and the like, and has the wide application prospect and the good economic value in the field of papermaking sewage treatment.

The invention discloses an oncomelania killing bacteria. The biological collection number of the oncomelania killing bacteria (comamonas sp.X3) is CCTCC M 2013442; and the oncomelania killing bacteria CCTCC M 2013442 is comamonas bacteria which is screened from moribund negative oncomelania and can quickly and effectively kill oncomelania. The bacteria provided by the invention is capable of killing oncomelania and can be directly used as a biological molluscacide; by comparing the biological molluscacide prepared from the oncomelania killing bacteria CCTCC M 2013442 with the traditional chemical molluscacide, the preparation method is simple and environment-friendly while the cost is low.

The invention belongs to the field of microorganisms, relates to a degradation bacterium W1 for propyzamide, and belongs to comamonas; the degradation bacterium is preserved in China Center for Type Culture Collection on October 11th, 2013, the preservation number is CCTCC NO. M2013464, and the registered number of 16S rRNA in Genebank is 1680505. The bacterium can degrade 90% of 100mg/L of propyzamide within 8 hours. The degradation bacterium is used for degrading herbicide propyzamide, and is used for biological purification of water, soil and agriculture products polluted by the propyzamide.

Disclosed is an immunopotentiating composition comprising an effective immunostimulating substance which can activate natural immunity and a subsequent immunity mediated by lymphocytes without causing any damage to cells. Specifically disclosed is an immunopotentiating composition which is characterized by comprising, as an active ingredient, an immunostimulating substance produced by decomposing at least one bacterium selected from an MRE symbiotic bacteria group consisting of Bacillus sp. (FERM BP-11209), Lysinibacillus fusiformis (FERM BP-11206), Bacillus sonorensis, Lysinibacillus sp. (FERM BP-11207) and Comamonas sp. (FERM BP-11208).

The invention provides a genetically engineered bacterium for rapidly degrading methyltestosterone and an application thereof. The process comprises the steps of knocking a PhaR gene of testosterone comamonas to obtain a high-expression engineering strain PK-4 capable of generating 3,17 beta-hydroxysteroid dehydrogenase by a gene homologous recombination technology; preparing bacterial suspension from the engineering strain PK-4, inoculating into waste water generated by livestock and poultry and aquatic product cultivation containing testosterone pollutant; cultivating for 48 hours when the temperature is 25 DEG C and the rotating speed is 150rpm, wherein the degradation rate of the methyltestosterone is 78.36-90.23%; the pH value is 6-8 in the waste water of which the methyltestosterone concentration is 1mg/L; the temperature is 15-25 DEG C; and cultivating for 48 hours at 150rpm in an oscillating manner, so that the degradation rate of the methyltestosterone can achieve 60.15%-90.23%. The genetically engineered bacterium has the beneficial effects that the engineering strain PK-4 of removed testosterone comamonas of the PhaR gene is obtained for the first time; and the genetically engineered bacterium is strong in adaptive capacity in swine wastewater, and fast in removal speed of the methyltestosterone, and has a great application value in the aspects of treatment and disposal of breeding waste and bioremediation of environmental pollution.

The invention discloses an anode photosynthetic solar fuel cell system additionally provided with degradation strains and application thereof. According to the anode photosynthetic solar fuel cell system, at least one of additionally added degrading bacteria, namely comamonas or bacillus, is added, and the abundance of electrochemical active microorganisms in soil is improved in a manner of externally connecting the degrading strain so that the capability of degrading surrounding environmental pollutants is improved, and the system has a good application prospect in treating Cr and NO3-N pollution in the soil.

The invention discloses a composite microbial agent capable of degrading agricultural wastes and belongs to the technical field of microbes. The active ingredients of the agent refer to the following strains separated from environments such as termite guts, composted straws and sediments of South China Sea: enterobacterium (Enterobacter sp.)YC105C, comamonas (Comamonas serinivorans) C35, rhodococcus (Rhodococcus sp.) L17 and bacillus (Bacillus ligniniphilus) L1. The composite microbial agent contains the concentrated bacterium powder of the following components in percentage by mass: 20-30% of enterobacterium, 30-40% of comamonas, 10-20% of rhodococcus and 20-40% of bacillus. The composite microbial agent is capable of degrading the agricultural wastes such as wheat straws, maize straws and green soybean straws, the quantity of the straws returned to the field is increased, and the cultivated land quality is improved, so that the growth of crops is promoted.

The invention discloses a method for promoting anaerobic digestion sludge heavy metal stability. The method comprises the steps that iron reducing bacteria are added in anaerobic digestion sludge, andanaerobic digestion is conducted for 20-60 days at 25-37 DEG C; the iron reducing bacteria are selected from at least one of humus reductive corynebacterium, iron reducing comamonas and klebsiella pneumoniae. According to the method, heavy metal can form stable minerals, and then rapid fixation of the heavy metal is achieved. In the iron reducing bacteria iron reduction process, mineralization oforganic matter is coupled, and meanwhile the sludge digestion process is directly accelerated.