11 results about "Comamonas" patented technology

The invention relates to a compound biological agent for soil remediation of northeast agriculture and forestry grassland by taking corn straw as a raw material as well as a preparation method and application of the compound biological agent. The compound biological agent is prepared from bacillus subtilis, paenibacillus mucilaginosus, saccharomycetes, Korean comamonas and an auxiliary agent. The corn straw is used as a main raw material, the composite biological agent is used for biological composting fermentation under the low-temperature condition in northeast, the prepared product has the unique characteristic of secreting polyglutamic acid and potassium under the low-temperature condition, the cost is low, the seed emergence rate is increased after application, the resistance of seedlings is enhanced, and the yield of the seedlings is increased. The soil physical and chemical properties can be effectively improved, the stress effect is reduced, effective nutrients in soil absorbed by crops are increased, and the crop yield is increased.

The invention belongs to the technical field of sewage treatment, and particularly relates to a preparation method of a low-temperature composite flora, a cold-resistant microbial agent for low-temperature sewage treatment and application of the cold-resistant microbial agent. The invention provides a low-temperature complex microbial community, which is prepared from flavobacterium (Flavobacterium), aeromonas (Aeromonas), sphingomonas (Sphingobacterium), pseudomonas (Pseudomonas), Comamonas (Comamonas), Massiella (Massiella) and Delftia (Delftia), and is characterized in that the low-temperature complex microbial community is prepared from the following raw materials: the flavobacterium (Flavobacterium), the aeromonas (Aeromonas), the sphingomonas (Sphingobacterium), the pseudomonas (Pseudomonas (Pseudomonas (Pseudomonas (Pseudomonas (Pseudomonas (Pseudomonas (Pseudomonas (Pseudomonas (Pseudomonas (Pseudomonas (Pseudomonas (Pseudomonas The low-temperature composite flora can treat domestic wastewater under a low-temperature condition, and is high in nitrogen removal rate, short in treatment time, wide in application range and better in impact resistance.

The invention discloses a mass spectrum method for rapidly identifying pantoea agglomerans, and solves the technical problems of high required cost and long consumed time in the prior art. The method comprises the following steps: S1, acquiring a pantoea agglomerans characteristic fingerprint spectrum; s2, according to the obtained pantoea agglomerans characteristic fingerprint spectrum, obtaining a mass-nucleus ratio and a corresponding weight of the pantoea agglomerans characteristic fingerprint spectrum; and S3, performing mass spectrum detection on unknown bacteria by using the obtained pantoea agglomerans characteristic fingerprint spectrum, comparing the mass spectrum of the unknown bacteria with the characteristic fingerprint spectrum, calculating a score, and judging whether the unknown bacteria are pantoea agglomerans or not according to a score result. According to the method disclosed by the invention, unknown bacteria can be identified in a short time by combining a mass spectrum technology with a characteristic fingerprint spectrum, so that the detection efficiency is greatly improved.

The invention provides comamonas and application thereof in removing soluble organic carbon, and relates to the technical field of sewage treatment. The Comamonas sp. WN2 strain disclosed by the invention is preserved in the Guangdong Microbial Culture Collection Center (GDMCC), and the preservation number is GDMCC NO. 64336. The Comamonas sp. WN2 strain disclosed by the invention has the advantages that the Comamonas sp. The comamonas strain WN2 provided by the invention has efficient organic carbon degradation capability, and can significantly reduce the total organic carbon and chemical oxygen demand level of a water body. The biological membrane generated on the basis of the comamonas strain WN2 is used for sewage treatment, so that the technical effects of improving the sewage treatment efficiency and effectively reducing the operation cost are achieved.

The invention discloses a compound microbial agent capable of efficiently degrading chlorfenapyr and application of the compound microbial agent, and belongs to the technical field of environmental microorganisms. The compound microbial agent is prepared from three strains of bacteria, namely, Brucella anthropi GXAS CMJ5, Shenotrophomonas maltophilia GXAS CMJ9 and Comamonas terrae GXAS CMJ10, according to a specific ratio, and the compound microbial agent is prepared from three strains of bacteria, namely, Brucella anthropi GXAS CMJ5, Shenotrophomonas maltophilia GXAS CMJ9 and Comamonas terrae GXAS CMJ10. The invention also provides a screening and construction method of the composite flora and an optimal condition for degrading chlorfenapyr. Under the optimal condition, the degradation rate of the composite flora on 150 mg / L chlorfenapyr is as high as 97.60%, and the strains are free from antagonism and high in environmental adaptability. The invention provides efficient, safe and economic special microbial resources and technical schemes for bioremediation of chlorfenapyr polluted soil, water bodies and agricultural products, and has wide application prospects.

The invention relates to the technical field of agricultural microorganisms. The invention discloses a strain of Comamonas odontotermes GZL 91, and the collection number of the Comamonas odontotermes GZL 91 is CGMCC (China General Microbiological Culture Collection Center) NO.36852. The invention further discloses a preparation method of the Comamonas odontotermes GZL 91. The strain GZL 91 is preserved in the China General Microbiological Culture Collection Center on November 27th, 2025, the Chinese name of the strain GZL 91 is called comamonas, the Latin name of the strain GZL 91 is Comamonas odontobacteria, the preservation number of the strain GZL 91 is CGMCC NO.36852, the preservation center is called CGMCC for short, and the address of the preservation center is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The base sequence of the strain GZL 91 is as shown in SEQ ID NO.1 in a sequence table. The composite microbial agent containing the strain is used for straw in-situ decomposition, the early-stage decomposition effect is remarkable, straw is powdery, the physicochemical property of soil can be remarkably improved, and the content of organic matter in the soil is increased by 15%; and the crop yield is increased by about 10%.

Disclosed herein are engineered P. putida KT2440 co-expressing PETase and MHETase enzymes that selectively degrades PET into monomers, ethylene glycol and terephthalate (TPA). In another embodiment, disclosed herein are methods for making and using a highly efficient EG metabolizing P. putida KT2440 strain. Given that native P. putida does not have a TPA metabolic pathway, nor the proteins to transport TPA into the cell, the next metabolic engineering challenge for developing synthetic P. putida strain to plastic upcycling was enabling TPA catabolismin P. putida KT2440. TPA transporters and catabolic pathway have been characterized in several microorganisms including Comamonas sp. strain E6 and Rhodococcus jostii RHA1.

The invention discloses a cadmium-tolerant comamonas strain and an application thereof. According to the present invention, a PBAT efficient degradation bacterium comamonas fluminis S261-2 strain is separated from the eisenia fetida intestinal tract content, the strain is preserved in Guangdong Microbial Culture Collection Center on April 7, 2025, and the preservation number is GDMCC No: 66102; researches show that the S261-2 strain has salt-tolerant and cadmium-tolerant capabilities, can grow in an environment with a PBAT mulching film as a unique carbon source, acts on the PBAT mulching film to initiate hydrolysis of the PBAT mulching film and cause breakage of molecular chains, so that the effect of degrading the PBAT mulching film is achieved. Meanwhile, the S261-2 strain can normally grow under the stress of 200 mg / L of cadmium ions and can promote degradation of PBAT mulching films, the degradation effect is good, the strain is suitable for bioremediation of plastic and heavy metal combined pollution sites, the environmental adaptability of the strain is higher, and the biological treatment cost can be reduced.

This invention discloses *Comamonas* sp. strain S1-158 and its applications. Strain S1-158 is a new species of *Comamonas* sp. capable of efficiently synthesizing volatile selenium, with accession number GDMCC No: 63731. The *Comamonas* sp. strain S1-158 of this invention exhibits strong tolerance to selenate, selenite, and elemental selenium, and can efficiently convert selenite and elemental selenium into volatile selenium. A method for the large-scale preparation of methylselenic acid using a bioreactor has also been established, which can be used for the treatment of selenium-containing wastewater and the bioremediation of selenium-polluted environments, showing broad application prospects.

The invention discloses a chlorohydrocarbon efficient synergistic degradation combined fungicide, a preparation method and application, and belongs to the technical field of microorganisms, the combined fungicide contains any one strain from rhodococcus or flavobacterium, and any one strain from bacillus, acinetobacter or comamonas. Through limitation of specific bacterium genus combination and artificial proportion optimization, the combined bacterium agent realizes a stable synergistic effect among different bacterium genus, the degradation efficiency is remarkably enhanced, and the 24-hour degradation rate of chlorinated hydrocarbon can be increased to 70% or above and is remarkably higher than that of a single bacterium system. Meanwhile, large-scale production can be achieved through the combined microbial agent, the overall cost is lower than that of a conventional scheme when the combined microbial agent is used for treating chlorinated hydrocarbon pollutants, additional secondary treatment is not needed after repairing is completed, and economic benefits are remarkable. When the combined microbial agent is used for treating chlorinated hydrocarbon pollutants, high-temperature treatment or chemical oxidation agents are not needed, and the combined microbial agent conforms to the development direction of current environmental protection laws and regulations and green restoration technologies.

This application relates to the field of agricultural microbiology, and in particular to a potassium-solubilizing and growth-promoting bacterial strain adapted to high-altitude and arid environments and its application. The strain was isolated from sandy soil in a recovery phase following degradation in the Ruoergai Grassland in a high-altitude and cold region. It was identified as a *Comamonas* strain by 16S rDNA gene sequencing and phylogenetic analysis. After 7 days of shaking culture in potassium-solubilizing liquid medium at 30°C and 180 r / min, the effective potassium content in the fermentation broth was 14.41 mg / L. The strain can grow in LB medium at 12°C, remains viable after freezing, and can also grow in LB solid medium containing 800 mM mannitol at 30°C. The strain is classified as *Comamonas sp ZSK1*, with accession number CCTCC NO: M 20252437, deposited on November 3, 2025, at the China Center for Type Culture Collection. The strain described in this application solves the technical problem of existing potassium-solubilizing bacteria being functionally inactive and having low colonization rates due to limited or insufficient stress resistance.