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Comamonas capable of effectively degrading picolinic acid and application of comamonas

A technology of Comamonas and picolinic acid, which is applied in the field of Comamonas that can efficiently degrade picolinic acid, can solve the problems of limitation of picolinic acid-degrading bacteria and rare strain reports, and achieve broad application potential and enhanced treatment effect of ability

Active Publication Date: 2016-11-16
XI AN JIAOTONG UNIV
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Problems solved by technology

There are few reports on strains of other genera
In addition, the picolinic acid-degrading bacteria reported in the literature all grow in a relatively mild pH range, basically in the range of 6.0 to 7.0, while the pH of the actual picolinic acid industrial wastewater varies widely, so the picolinic acid-degrading bacteria reported in the literature Practical applications are limited

Method used

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  • Comamonas capable of effectively degrading picolinic acid and application of comamonas
  • Comamonas capable of effectively degrading picolinic acid and application of comamonas
  • Comamonas capable of effectively degrading picolinic acid and application of comamonas

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Embodiment Construction

[0021] The present invention will be further described in detail below in conjunction with the technical scheme and the accompanying drawings.

[0022] 1, Comamonas provided by the invention, its screening steps are as follows:

[0023] Aerobic activated sludge samples were collected from the aeration tank of a pharmaceutical factory in Xi'an, and used as a source of bacteria for domestication and cultivation. Add 0.2% sodium pyrophosphate to 50mL of activated sludge as a deflocculant, shake on the shaker for 5-10min to break up the activated sludge flocs, take 5mL of activated sludge, add 95mL of inorganic salt medium to shake In the bottle, add picolinic acid as the only carbon, nitrogen, and energy source, wherein the concentration of picolinic acid is 100 mg / L, and culture on a shaking table at a temperature of 30° C. and a rotation speed of 180 r / min. Inorganic salt medium composition: Na 2 HPO 4 12H 2 O, 7g / L; KH 2 PO 4 , 1g / L; CaCl 2 2H 2 O, 10mg / L; FeCl 3 , 2m...

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Abstract

The invention provides comamonas capable of effectively degrading picolinic acid and an application of the comamonas, and belongs to the technical field of bioengineering. According to the invention, a picolinic acid degrading bacterium, namely comamonas, is separated and screened out, and the comamonas is classified and named as comamonas sp., which is preserved in China General Microbiological Culture Collection Center with preservation number of CGMCC NO.10700. The comamonas disclosed by the invention has the advantages that the comamonas is capable of effectively degrading the picolinic acid within a relatively wide pH value range, in particular under the circumstance of relatively high alkalinity; and the comamonas, as a biological enhancing preparation, can be applied to aerobic biological treatment of picolinic acid wastewater in weak base and weak acid environments; therefore, the comamonas has a broad application prospect.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a Comamonas bacterium capable of efficiently degrading picolinic acid and its application. Background technique [0002] Picolinic acid and its derivatives are widely used in the production of medicines, pesticides and daily chemicals, and the widespread use of picolinic acid leads to environmental pollution. Picolinic acid wastewater can be treated by aerobic biological method. At present, the microbial resources for aerobic degradation of picolinic acid mainly include Arthrobacter, Mycobacterium and Streptomyces. There are few reports on strains of other genera. In addition, the picolinic acid-degrading bacteria reported in the literature all grow in a relatively mild pH range, basically in the range of 6.0 to 7.0, while the pH of the actual picolinic acid industrial wastewater varies widely, so the picolinic acid-degrading bacteria reported in the literatu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/38
CPCC02F3/34C02F2101/38C12R2001/01C12N1/205
Inventor 郑春莉沈振兴何炽刘萍萍刘红霞冯珊珊王巧蕊
Owner XI AN JIAOTONG UNIV
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