416 results about "Domestication" patented technology

The invention provides a digital twinborn monitoring, regulating and controlling method for a copepod bait biological domestication process. The method comprises the following steps: establishing a micro-ecology and population dynamics coupled twinborn model, and defining a monitoring parameter and control object unified term system; collecting multi-source sensing data at an edge end, and performing clock synchronization, signal denoising and state estimation to obtain a micro-ecology, group and water body state joint estimation result; executing parameter online identification based on state estimation, and constructing a safety constraint set of ammonia nitrogen, dissolved oxygen, death rate, environment change rate and the like; the breeding efficiency improvement and the system stability balance are realized through a multi-objective optimization function; and generating a micro-ecological pulse and environment gradual change cooperative control action set according to a solving result, and when a set condition is met, outputting a domestication prescription and terminating the process. Intelligent, low-stress and high-stability regulation and control of a copepod domestication process are realized through digital twin modeling, rolling predictive control and a self-evolution updating mechanism.

The invention relates to a mycorrhizal cultivation method for improving the stress resistance of pinus khasys seedlings, and belongs to the technical field of agricultural planting, the cultivation method comprises the following steps: (1) compounding fibrous belly fungi, bolete and russule virens into a microbial inoculum, and culturing to prepare microcapsules; (2) taking turf, vermiculite, humus soil and perlite as substrates, and adding a microcystis agent and a water-retaining agent; (3) the seeds are disinfected and sown, and seedlings are domesticated through light intensity gradient, water stress and temperature fluctuation; 4, during transplanting, abscisic acid is used for treating root systems, organic fertilizer is pre-filled, and mycorrhizal activation promoting liquid is perfused, through the synergistic effect of composite mycorrhizal fungi and stress domestication, the stress resistance, the survival rate and the afforestation adaptability of pinus khasys seedlings are remarkably improved.

The invention discloses a method for establishing a medicinal plant litsea cubeba tissue culture seedling system. Aiming at the problems of difficulty in explant pollution and browning control, low proliferation and rooting efficiency, insufficient transplanting and domesticating survival rate and the like in establishment of a litsea cubeba tissue culture system in the prior art, the invention provides the establishment method of the medicinal plant litsea cubeba tissue culture seedling system, the germination rate of explants is 90% or above, and the transplanting survival rate in 30 days reaches 90.6%.

The invention provides a sandy soil root domestication layered structure improvement method which comprises the steps that a surface guide layer, a channel induction layer and a deep resource layer are sequentially arranged on the earth surface from top to bottom, in-situ sandy soil is buried in the surface guide layer, a loose channel is constructed in the channel induction layer, and the loose channel is communicated with the deep resource layer; the deep resource layer comprises a water locking substance, a water retention substance, an adsorption substance and a supporting substance; when it is detected that the EC value of the joint of the surface guide layer and the channel induction layer is larger than the preset multiple of the EC value of the joint of the channel induction layer and the deep resource layer, a drip irrigation system is started to conduct drip irrigation on the surface guide layer according to the preset water pressure and water flow so as to guide nutrients in the surface guide layer to be collected towards the deep resource layer; according to the method, replacement of soil dressing is not needed, deep pricking and field planting of plant roots are guided through water and fertilizer stress and nutrition induction mechanisms, the drought resistance and stress resistance of plants are improved, the quality of crop products is optimized, a stable water and fertilizer environment is created for growth of plants in sandy soil, and the economical efficiency and effectiveness of the improvement technology are improved.

The invention relates to marine fish breeding, and particularly discloses an indoor full-artificial breeding method for black epinephelus, which specifically comprises the key technical links of nutrient enrichment of parent fish, hormone induced spawning, artificial insemination and fertilized egg incubation, indoor breeding of fry, compound feed domestication and the like. By controlling water temperature, illumination, salinity and nutritional conditions, synchronous maturation and efficient ovulation of parent gonads of the epinephelus nigatosus are achieved, the fertilization rate reaches 95% or above, and the hatching rate exceeds 85%. According to the method, the whole-process artificial controllable breeding of the epinephelus nigatosus from the parent fish to the fry is realized, a new variety is provided for the epinephelus culture industry, and a feasible technical approach is also provided for the artificial breeding and improved variety breeding of the wild epinephelus nigatosus in the island reef of the South China Sea.

The invention belongs to the technical field of mandarin fish culture, and relates to a mandarin fish domestication survival rate prediction method and system based on ecological simulation. Establishing a quantitative relation between the ecological factors and the sample mandarin fish survival rate change slope based on the quantitative relation so as to form an evaluation model; a historical survival rate curve is generated, and the risk state is determined by comparing the current sample mandarin fish survival rate change slope with the slope of the historical survival rate curve in the corresponding time section; calculating a change path of the ecological factors in a future time period based on the evaluation model, generating a predicted survival rate curve, and outputting an analysis report containing a risk state judgment result; according to the method, a complex domestication process is converted into a quantifiable data model, and early-stage accurate early warning of the risk is realized through dynamic analysis of the change trend of the survival rate.

A preparation method of an ionic rare earth leaching agent includes the following steps: (1) domestication microorganisms with rare earth activated mineral powder culture medium to obtain a microbial suspension; (2) amplifying and culturing the microbial suspension and additives to obtain the amplified culture medium; and (3) mixing the modified sesbania gum with the amplified culture medium to obtain the ionic rare earth leaching agent. The activated mineral powder is the active metal-containing mineral powder in nature, which has excellent cation exchange function after activation, and the activated mineral powder and ionic rare earth mineral powder are used as the medium components to domesticate microorganisms, so that microorganisms can survive in the above-mentioned ionic solution and improve the leaching rate of synergistic leaching ionic rare earth.

The invention provides a method for preparing rare ginsenoside through conversion of kombucha, the rare ginsenoside and application, and belongs to the technical field of biological medicine. A symbiotic system is formed by multiple bacteria such as acetic acid bacteria, gluconacetobacter, foal bacillus and the like and fungi, after the symbiotic system is domesticated, the American ginseng stem and leaf extract is fermented through multi-bacteria synergistic catalysis, the rare ginsenoside can be efficiently prepared, the highest conversion rate of Rb3 and Rd can reach about 90%, and the yield of Rb3 and Rd can reach about 90%. Meanwhile, the contents of rare ginsenosides Rg3 (20S), Rg5, Rh2 (20R) and Rh2 (20S) are remarkably increased and are increased by about 15 times at most, and efficient conversion of various ginsenosides is achieved.

The invention relates to the technical field of soil improvement and microorganisms, and discloses a preparation process of a porous activated carbon soil conditioner loaded with microbial flora, which comprises the following steps: firstly, performing high-temperature activation and acid-base modification on a biomass raw material to prepare a porous activated carbon carrier, and dipping in a nutrient solution to preset a slow-release nutrient substrate; secondly, adopting a step-by-step sequential loading process, firstly loading the bacillus subtilis subjected to stress resistance domestication on a carrier through electrostatic adsorption, and performing activation colonization culture to form a stable pioneer biological membrane; then, on the basis of the biological membrane, phosphate solubilizing bacteria, potassium solubilizing bacteria and antagonistic bacteria are loaded in sequence; finally, the loaded composite carrier is subjected to microcapsule embedding and low-temperature drying treatment, and the porous activated carbon soil conditioner is obtained. According to the modifier prepared by the invention, through ordered loading and multiple protection, the problem of strain competition is solved, the high survival rate of flora is ensured, and the improvement effect is stable and long-acting.

The invention discloses a biochar-based microbial soil conditioner and a preparation method thereof, and relates to the technical field of soil improvement. The preparation method comprises the following steps: firstly, carrying out alkali-acid treatment and segmented pyrolysis on raw materials such as wood chips to obtain primary biochar, and then carrying out chemical activation, nano material modification and plasma treatment to obtain the composite modified biochar. Meanwhile, probiotics such as bacillus subtilis and the like are subjected to activation, expanding culture and saline-alkali domestication to obtain directional domestication bacterial liquid, and the directional domestication bacterial liquid is solidified into microspheres by adopting a composite embedding carrier. And finally, mixing the composite modified biochar, the composite embedded microspheres, potassium humate and other auxiliary materials, drying, and sieving to obtain the soil conditioner. The soil conditioner disclosed by the invention can realize an improvement effect on saline-alkali soil and reduce the total salt content; meanwhile, directionally domesticated microorganisms secrete organic acid to neutralize alkaline components, the pH of the soil is adjusted to the range suitable for crop growth, and salt and alkali stress is relieved.

The invention discloses a staged regulation and control sulfur cycle enhanced denitrification microbial community construction method, and belongs to the technical field of environmental biologication.The method comprises the three stages that in the first stage, low-carbon-nitrogen-ratio domestication culture is conducted, the carbon-nitrogen ratio is 1.5-2.5: 1, and domestication is conducted for 50-70 days; in the second stage, electron donor-acceptor ratio regulation culture is carried out, the / molar ratio is (4-6): 1, and culture is carried out for 15-25 days; in the third stage, pulse type sulfate is fed for enhanced culture, the single-time feeding concentration is 200-500 mg / L, culture is conducted for 8-15 days, real-time monitoring is conducted through the fluorescence in-situ hybridization technology, construction is completed when the abundance of sulfur autotrophic denitrifying bacteria reaches 28% or above, and when the microflora constructed through the method is used for treating low-carbon-nitrogen-ratio industrial wastewater, the sulfur conversion rate is 1.2-2.0 kg / (m.d), the system stability is good, and the application range is wide. And the impact load resistance is high.

A compartment ditch three-dimensional frog breeding and feeding device relates to the technical field of ecological breeding and comprises a feeding table arranged on a compartment ditch, the feeding table comprises an elastic net used for feeding food and a supporting structure arranged on the edge of the elastic net, and the supporting structure is erected on the upper edge of the compartment ditch. The mesh size of the elastic net allows fine particles in the food to fall into the ditch below the elastic net. The cage ditch three-dimensional frog feeding device is suitable for cage ditch three-dimensional frog feeding, centralized feeding is carried out by arranging the feeding table above the cage ditch, and the food feeding efficiency is improved. The device has the innovative functions of adapting to field water depth management in different growth periods under ditch type rice cultivation, improving the feeding domestication efficiency, trapping field pests as frog bait and the like. According to the rice and frog symbiosis method, the mode that in traditional rice and frog symbiosis, feeding is conducted on the periphery of a farmland is improved, land resources are saved, multi-layer utilization of land is innovatively achieved, the agricultural output value is increased, and the requirement for the proportion of the crop planting area can be met.

The invention discloses application of an SNP (Single Nucleotide Polymorphism) molecular marker related to the character of siniperca chuatsi in feeding artificial compound feed, and belongs to the field of biotechnology and aquatic animal genetic breeding. The SNP molecular marker is located at the 29390304 site from the 5'end on the 10 # chromosome of the siniperca chuatsi genome, is A or T, and is easy to domesticate when the genotype is AA; or the SNP molecular marker is located at the 1218488 site from the 5'end on the 20 # chromosome of the siniperca chuatsi genome, and is easy to domesticate when being the genotype of T or A or TT. The SNP molecular marker is closely related to the artificial compound feed intake capacity of the siniperca chuatsi, so that the feeding domestication potential of the siniperca chuatsi can be predicted in an early stage through the SNP molecular marker, or molecular marker-assisted breeding is performed through the SNP molecular marker, the proportion of individuals easy to domesticate in a group is increased, the breeding cost is remarkably reduced, and the breeding efficiency is improved. The economic benefit is improved.

The invention relates to the technical field of biological sewage treatment, and particularly discloses a process for combined treatment of antibiotic wastewater by a compound microorganism-MBR (Membrane Bioreactor) membrane. The invention provides a process for combined treatment of antibiotic wastewater by a compound microorganism-MBR (Membrane Biological Reactor) membrane, which comprises the following steps of: performing directional domestication and toxicity screening on activated sludge through COD (Chemical Oxygen Demand) gradient increase and decrease and removal rate control, and constructing a microbial community resistant to sulfamethoxazole impact; further, a compound bacteria solution composed of marine Arfiv bacteria and Rhodopseudomonas faecalis is introduced, so that synergistic degradation of high COD and sulfamethoxazole is realized under a proper pH condition, and the COD removal rate is still kept at a relatively high level when the concentration is increased; and the tail end is matched with MBR membrane separation, and efficient biodegradation and efficient solid-liquid separation are coupled, so that the sulfamethoxazole and COD in the wastewater are efficiently removed under the conditions of low drug consumption and near neutrality.

The invention discloses a domestication method for efficiently degrading tobacco waste to produce hydrogen by using mixed flora, which comprises the following steps: carrying out anaerobic enrichment domestication culture by taking manure compost as a bacterial source and cellulose prepared from tobacco waste as a core component of a domestication culture medium; the domestication culture medium contains cellulose, ammonium bicarbonate and monopotassium phosphate, and the cellulose concentration gradient is gradually increased without adjusting the pH value; stopping culturing when the hydrogen content in the biogas is reduced by 10%, transferring the biogas to a fresh domestication culture medium for passage according to an inoculum size of 10-20%, and continuously domesticating for 20-40 rounds; inoculating the domesticated mixed flora culture solution into a cellulose liquid fermentation culture medium containing microcrystalline cellulose, ammonium bicarbonate, monopotassium phosphate and a nutrient solution according to the inoculum size of 10-20%; the initial pH of the fermentation liquor is adjusted to 6.5-7.5, high-purity nitrogen is introduced to create an anaerobic environment, and hydrogen is produced through fermentation under the conditions of 35 + / -1 DEG C and 120 r / min. According to the method, flora is easy to culture, hydrogen production is efficient and stable, tobacco waste resources can be utilized, flora stability is high, adaptability is high, and strict sterile conditions are not needed.

The application discloses a mandarin fish domestication pool, which comprises a pool body, wherein an oxygenation device, a water inlet and a water outlet are arranged in the pool body, a bait fish breeding frame is arranged below the water inlet, and a feeding device is arranged on the side of the bait fish breeding frame; the feeding device comprises a feeding pipe, a bait temporary storage tank arranged on the upper end of the feeding pipe, a feeding hopper arranged on the upper end of the bait temporary storage tank, cathode ring pieces and anode ring pieces arranged in the feeding pipe, and a pulse generator and a pulse power amplifier connected through wires. The domestication pool is improved, so that live fish feeding, live fish and dead fish or feed proportionate feeding are facilitated, and switching and adjustment are facilitated, thereby improving the convenience and success rate of domestication.

The invention provides a breeding method suitable for breeding litopenaeus vannamei in saline-alkali soil, and relates to the technical field of aquaculture. According to the breeding method suitable for breeding the litopenaeus vannamei in the saline-alkali soil, the proportion of Ca < 2 + > to Mg < 2 + > to K < + > in a water body is optimized to be 2.8: 1: 0.3 by customizing a compound ion regulation and control solution, SO4 < 2-> / Cl <-> is controlled to be 0.14-0.8, and the pH is stabilized to be 8.2-8.8 in combination with a citric acid-sodium citrate buffer system; a slow-medium-fast segmented domestication strategy is adopted to adapt to different development stages of the fries; ethanol clostridium protein (CAP) is added into the feed to be combined with compound probiotics, so that immune metabolism regulation and control are enhanced. Experiments prove that according to the method, the survival rate of the litopenaeus vannamei seedlings reaches 86.3% + / -2.1%, the culture period is shortened by 12-15 days, the hepatopancreas SOD activity is improved by 42.6%, the vibrio inhibition rate reaches 78.5%, and the method is remarkably superior to the prior art. The method does not need large-scale pond transformation, adapts to different types of saline-alkali soil water bodies, realizes efficient green culture, and has important application value.

The application discloses a kind of gradient low temperature domestication, constant low temperature storage method of acrosternum hilare adult, comprising the following steps: (1) 10-15 day old acrosternum hilare adult is placed into storage box, the substrate and armyworm are loaded in the storage box;(2) the storage box is placed into artificial climate chamber and gradient low temperature domestication is carried out, and the gradient low temperature domestication program is: 26 DEG C →20 DEG C domestication 8h →15 DEG C domestication 8h →10 DEG C domestication 8h →6 DEG C domestication 8h →4 DEG C domestication 8h;(3) after domestication, acrosternum hilare adult is placed at 4 DEG C and refrigerated, and refrigeration time is 40-60 days.The application can guarantee normal fecundity and egg hatch rate, and better offspring development and offspring survival rate in its life period by placing acrosternum hilare adult into storage box loaded with substrate and armyworm and carrying out gradient low temperature domestication, and then placing it at 4 DEG C and refrigerating for 40-60 days.

The invention provides a fry adaptive domestication system and method based on multidirectional flow impact exercise, and relates to the technical field of fishery breeding, the system comprises three-section domestication pool bodies, the domestication pool bodies are detachably connected through flange plates, the bottom of each domestication pool body is fixedly connected with a supporting column, the upper portion of each supporting column is fixedly connected with a supporting seat, and the supporting seat is fixedly connected with the bottom of the corresponding domestication pool body. A motor is arranged on the upper portion of the supporting seat, a third bevel gear is fixed to the output end of the motor, and a connecting assembly facing the side wall of the domestication pool body in the radial direction is meshed with the third bevel gear. The invention provides a multi-directional flow impact training structure, which enables the fry to swim back and forth, turn and avoid and the like in radial, annular and spiral flow, the caudal peduncle muscle fiber density is improved by 20-30%, the swimming speed is improved by 40% compared with that of the traditional domesticated fry, the anti-countercurrent ability intensified training in the three-dimensional space of the fry is realized, and the endurance, explosive power and turning flexibility of the fry are improved in a balanced manner.

The invention relates to the technical field of biological breeding, and discloses a composite microecological preparation for adjusting lamb intestinal flora and relieving weaning stress, the composite microecological preparation comprises, by weight, 10-40 parts of enterococcus faecalis powder, 10-40 parts of bacillus subtilis powder and 10-40 parts of saccharomycetes powder, the enterococcus faecalis powder is prepared through a gradient acid-resistant pre-domestication system, and the bacillus subtilis powder is prepared through a gradient acid-resistant pre-domestication system. The bacillus subtilis powder is prepared through an oxygen-controlled induced spore strengthening process, the saccharomycetes powder is prepared through a beta-glucan strengthening reactor, and the three kinds of bacterium powder are mixed under the sterile condition to obtain the bacillus subtilis powder, the saccharomycetes powder and the beta-glucan strengthening reactor. The tolerance, activity and stability of the strain are improved, the controllable and repeatable preparation process of the compound microecological preparation is ensured, and a reliable technical basis is provided for regulating intestinal flora of weaned lambs and relieving weaning stress.

The present application belongs to the technical field of plant tissue culture, and particularly relates to a high-efficiency rooting and seedling raising method for plant tissue culture seedlings, S1 trimming and disinfection of S2 induction liquid immersion, S3 composite substrate implantation and gradient environment regulation, S4 substrate transplanting and planting; the core is to use modified magnetic biochar composite substrate loaded with FeO nanoparticles, cooperate with the synergistic effect of microencapsulated rooting hormone and melatonin, realize in-situ integrated seedling raising through composite spectrum containing UV-A, CO pulse supply, gradient humidity and PEG osmotic pressure domestication. The present application effectively inhibits basal browning, shortens the seedling raising period, improves the rooting quality and transplanting survival rate, overcomes the pollution prejudice of magnetic nanoparticles, is suitable for woody and herbaceous plants, and can be applied to plant tissue culture seedling production on a large scale.

The invention relates to a microbial agent for degrading COD (Chemical Oxygen Demand) in DBP (Dibutyl Phthalate) wastewater as well as a preparation process and application of the microbial agent. The microbial agent is prepared from the following active components in parts by weight: 88 to 91 parts of pseudomonas, 5.2 to 5.6 parts of enterobacter and 0.05 to 0.11 part of alcaligenes. According to the application, a plurality of microbial strains are formed in the microbial liquid which is subjected to mixed domestication culture of the DBP wastewater, the anaerobic sludge, the hydrolytic acidification bacteria and the nutritive salt, and a plurality of microbial strains in the microbial agent which is obtained by mixing the microbial liquid after bacteria screening and expanding culture have a synergistic effect, so that the microbial agent has the performance of efficiently degrading COD in the DBP wastewater in an environment-friendly manner.

The application discloses a striped jack fry hatching device and a hatching domestication method thereof, and aims to provide a striped jack fry hatching device and a hatching domestication method thereof, which are convenient to operate, can effectively reduce physiological stress and external abrasions of striped jack fry, and are favorable to improving the survival rate of the fry after releasing. The striped jack fry hatching device comprises a hatching net cage provided with a fry outlet on one side, vertical guide rods arranged on the left and right sides of the fry outlet, a telescopic net cover, a closing rope and a lifting rope.

The present invention relates to the technical field of aquaculture and breeding of seawater fishes, in particular to an industrialized cultivation method for cephalopholis sonnerati fry, comprising the steps of successively subjecting parent fishes of cephalopholis sonnerati to feed domestication and nutrient enrichment at a water temperature of 25-29°C and under a flowing water condition, so as to obtain parent fishes with fully developed gonads, etc. The present invention takes strict measures for fry quality control and infectious disease prevention and control during industrialized workshop cultivation, so as to effectively optimize the growth conditions of fry, thus increasing the growth rate and improves the health conditions and adaptability of fry while reducing the risk of diseases. By means of standardized breeding management and technical means, the growth environment and feeding management of fry can be controlled, thus effectively improving production efficiency. The cultivated cephalopholis sonnerati has the advantages of fast growth rate, high disease resistance, strong vigor, tender and delicious flesh and good market prospects.

The invention discloses a leiocassis longirostris fry large-scale cultivation method based on a facility circulating water system, and relates to the field of artificial breeding, and the method comprises the steps of indoor parent intensive cultivation, fertilized egg batch acquisition, fry incubation in a loop incubation pond, fry large-scale cultivation and the like. And water purification treatment and efficient oxygenation are realized. Different circulating water operation modes are adopted in the seedling breeding and domestication cultivation process, the optimal breeding ecological condition is provided, and the process is controllable. According to the Leiocassis longirostris fry hatching and breeding method, the Leiocassis longirostris fry hatching and breeding based on circulating water are adopted for Leiocassis longirostris fry large-scale breeding of a facility circulating water system, accurate control over the Leiocassis longirostris hatching process can be achieved, water quality stability is achieved, the water temperature can be accurately controlled, and water changing and wastewater discharging in the whole process are avoided.

The invention provides an antibacterial peptide prepared by utilizing copper-loaded lactobacillus plantarum, which is prepared by inoculating high-copper-loaded duck-origin lactobacillus plantarum into a culture medium for fermentation and then using fermentation liquor, and the preservation number of the copper-loaded duck-origin lactobacillus plantarum is CGMCC (China General Microbiological Culture Collection Center) NO.32835. The lactobacillus plantarum obtained through domestication can tolerate high-concentration copper ions, the lactobacillus plantarum obtained through domestication and breeding can stably grow, and metabiotics generated through fermentation of the lactobacillus plantarum contain small peptides, small molecular organic acids, digestive enzymes and the like. Due to the fact that the antibiotic peptide rich in the afterbiogen has an obvious inhibiting effect on harmful bacteria of waterfowls, the prepared antibiotic peptide preparation effectively improves the antibacterial activity, and as a feed additive, the antibiotic peptide preparation is beneficial to elimination of inflammatory factors of intestinal tracts of poultry, maintenance of intestinal health, increase of the utilization rate of feed and improvement of the product quality.

The application discloses a wild hundred-petal grass domestication cultivation method and particularly relates to the field of wild plant cultivation technology, and comprises the following steps: step one, wild resource investigation and conservation, resource investigation is conducted on wild populations of hundred-petal grass, accompanying plants, original habitat types, soil conditions, illumination conditions, humidity conditions and distribution characteristics are recorded, germplasm resources of different populations are synchronously collected, including seeds and rhizomes, and the collected samples are numbered, stored in different zones and registered in traits, a seedling test area and a germplasm resource basic file are established, and basis is provided for subsequent directional domestication. The application improves seed germination rate and seedling uniformity, also improves transplanting survival rate and field adaptability, realizes wild resource protection and sustainable utilization, constructs an ecological planting mode suitable for local climate, improves stability and controllability of medicinal material quality, reduces chemical input and meets green development requirements.

The application discloses a tissue culture propagation method of eriochloa contracta. The creeping stem section of the eriochloa contracta is collected, washed, and then sterilized and dried; the sterilized and treated stem section is inoculated into a bud induction medium of MS+1-1.5 mg / L 6-BA+0.1-0.5 mg / L NAA+30 mg / L sucrose+6.5 mg / L agar to perform bud induction, and after 15-20 days of culture, the adventitious bud seedling is obtained; the adventitious bud of about 1-1.5 cm induced in the primary culture medium is inoculated into a proliferation culture medium to perform subculture, and the next step can be divided into single bud expansion or direct transplanting domestication. The two-step method only needs primary culture-proliferation and rooting to be simultaneously performed, and the transplanting survival rate is high.

The invention discloses a growth-promoting methylobacterium R5018 strain and an application of the growth-promoting methylobacterium R5018 strain. The strain R5018 is obtained by separation from a rice phyllospheric microflora and methanol domestication and screening, and is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M20252544. The rice growth-promoting microbial inoculum prepared from the strain can be stably colonized at phyllosphere and promote the growth of overground and underground parts of rice through seed soaking, foliage spraying or combined application of the seed soaking and the foliage spraying, and the ear number, the grain number and the yield are remarkably increased. Compared with the prior art, the strain disclosed by the invention is derived from a rice phyllospheric bacterial community, is high in adaptability, environment-friendly and efficient, provides a new microbial technical scheme for high-yield cultivation of rice, and has a wide popularization and application prospect.

A method for rapidly restoring Phragmites australis population in degraded land through stolon domestication is provided, including the following steps: at the beginning of the Phragmites australis growing season from May to June every year, the Phragmites australis is made to crawl by draining water and artificially crushing the aboveground stems, and then the sprouting of stolon internode buds or root buds is promoted by continuously controlling water; in mid-July, suitable stolons containing internode buds or root buds are selected, and the root segments are dug from the base, and the stolons are wound in a circle in the extension direction and fixed, and sprayed with water to keep moisture; the fixed stolons are unfolded and transplanted into degraded land for daily water and fertilizer management.