535 results about "Domestication" patented technology

The invention provides a digital twinborn monitoring, regulating and controlling method for a copepod bait biological domestication process. The method comprises the following steps: establishing a micro-ecology and population dynamics coupled twinborn model, and defining a monitoring parameter and control object unified term system; collecting multi-source sensing data at an edge end, and performing clock synchronization, signal denoising and state estimation to obtain a micro-ecology, group and water body state joint estimation result; executing parameter online identification based on state estimation, and constructing a safety constraint set of ammonia nitrogen, dissolved oxygen, death rate, environment change rate and the like; the breeding efficiency improvement and the system stability balance are realized through a multi-objective optimization function; and generating a micro-ecological pulse and environment gradual change cooperative control action set according to a solving result, and when a set condition is met, outputting a domestication prescription and terminating the process. Intelligent, low-stress and high-stability regulation and control of a copepod domestication process are realized through digital twin modeling, rolling predictive control and a self-evolution updating mechanism.

The invention discloses a kitchen waste oil-based sophorolipid high-yield candida utilis strain and an application thereof. Belongs to the technical field of directional domestication breeding. According to the method, wild candida bombicola is used as an original strain, and is sequentially domesticated in a culture medium containing kitchen waste oil with the concentration gradient of 2%, 4%, 6%, 8% and 10% to obtain the S.bombicola x10. The invention further discloses an application of the domesticated strain in fermentation production of sophorolipid, after the strain is subjected to shake flask fermentation, the sophorolipid yield reaches 60.19 g / L and is improved by 16.22% compared with that of a wild strain, and the acid type sophorolipid yield is improved by 32.11%. When the concentration of the lactone sophorolipid generated by fermentation is 1.25 mg / mL, the growth of the bacillus subtilis is completely inhibited. The invention provides an excellent production strain for industrial production of sophorolipid, and has good theoretical research significance and practical application value.

According to the microbial flora culture method for efficiently degrading the organic pollutants, three continuous culture stages of enrichment, domestication and optimization are adopted, parameters such as the temperature, the pH value, the rotating speed and the ventilatory capacity are accurately controlled in each stage, and growth of microorganisms with degradation capacity is promoted. Through multi-stage culture and domestication, the obtained flora has efficient degradation capability on organic pollutants such as petroleum hydrocarbons, pesticides and polycyclic aromatic hydrocarbons, and has strong adaptability to pollutants with different concentrations. The 16S rRNA gene is used for sequencing and identifying the flora, and composition and dominant strains are clear. The flora is prepared into a microbial inoculum, and the microbial inoculum is preserved by freeze drying or liquid nitrogen, so that the activity can be effectively maintained. The flora can be widely applied to the fields of polluted soil and water body remediation, industrial wastewater treatment and the like, is green and environment-friendly, is relatively low in cost, avoids secondary pollution, conforms to the concept of sustainable development, and has relatively high economic and environmental values.

The invention belongs to the technical field of molecular breeding of aquatic products, and particularly relates to an Indel molecular marker for identifying production places of finless eels and application thereof, which are particularly suitable for identifying finless eel strains in Jiangxi regions and non-Jiangxi regions. The Indel molecular marker for identifying the producing area of the ricefield eel is an Indel molecular marker B, and the Indel molecular marker B contains nucleotide sequences as shown in SEQ ID NO: 3 and SEQ ID NO: 4. The monopterus albus strains in Jiangxi regions and non-Jiangxi regions can be rapidly identified by utilizing the molecular marker, genetic identification can be rapidly realized, domestication breeding of wild germplasm resources is accelerated, and precise breeding is realized.

The invention relates to the technical field of fishery breeding, in particular to a step depth adjustable mandarin fish domestication breeding water temperature detection device and a domestication method.The step depth adjustable mandarin fish domestication breeding water temperature detection device comprises a mounting plate; a lifting assembly capable of driving a lifting piece arranged on the mounting plate to intermittently descend in the longitudinal direction is arranged on the mounting plate; triggering pieces are arranged on the mounting plate at equal intervals. A temperature measuring cylinder is arranged on the lifting part, the temperature measuring cylinder is communicated with a water sample extracting assembly on the lifting part, the water sample extracting assembly comprises a suction cylinder which is arranged on the lifting part and is communicated with the temperature measuring cylinder, a suction part is arranged in the suction cylinder, and the suction part is matched with a driving assembly on the lifting part and can suck a water sample into the temperature measuring cylinder; when the driving assembly is separated from the triggering piece and is quickly reset, the suction piece acts reversely, and the sample pumped into the temperature measuring cylinder is discharged; through cooperation of all the assemblies, culture personnel can conveniently and accurately obtain the temperatures of water bodies at different depths of a culture pond.

The invention discloses a salt-tolerant reed seedling and a cultivation method thereof. The method comprises the following steps: seed selection and pretreatment; performing exogenous induction treatment; performing germination culture; salt gradient domestication culture; salt tolerance screening, seedling hardening and transplanting. According to the invention, by combining exogenous induction and salt gradient domestication culture, the stress resistance of the plant is regulated, and the salt tolerance mechanism in the seed is activated, so that a foundation is laid for better adaptation and growth in a high-salt environment subsequently; the salt-tolerant reed seedlings induced and domesticated by the method are high in seedling rate, the survival rate of acclimatization and transplantation is 90% or above, the breeding cycle is shortened to be within 100 days, the cultivation cycle is short, and the method is suitable for large-scale popularization and application.

The invention belongs to the technical field of molecular breeding of aquatic products, particularly relates to an Indel molecular marker for identifying ricefield eel strains and application thereof, and is particularly suitable for identifying ricefield eel strains in Jiangxi regions and non-Jiangxi regions. The Indel molecular marker for identifying the ricefield eel strain is an Indel molecular marker A, and the Indel molecular marker A contains nucleotide sequences as shown in SEQ ID NO: 3 and SEQ ID NO: 4. The monopterus albus strains in Jiangxi regions and non-Jiangxi regions can be rapidly identified by utilizing the molecular marker, genetic identification can be rapidly realized, domestication breeding of wild germplasm resources is accelerated, and precise breeding is realized.

The invention discloses a wetland system plastic pollution intelligent degradation system based on artificial intelligence driving, and relates to the field of ecological environment protection and artificial intelligence fusion. The problems that an existing wetland system lacks real-time monitoring and predicting capacity for the migration behavior of the micro-plastics, the combination of a microbiological degradation technology and the wetland system is not tight enough, and efficient degradation of the micro-plastics is difficult to achieve are solved. According to the system, an AI behavior path prediction model is adopted to predict a micro-plastic migration track and a micro-plastic distribution probability corresponding to the migration track, a structure optimization subsystem is utilized to optimize wetland structure parameters and filler distribution, and a directional domestication microorganism technology is combined to enhance the degradation capacity. The method is mainly applied to micro-plastic degradation of a wetland system.

The utility model relates to the technical field of aquaculture, and discloses a domestication device for artificial fish compound feed, which comprises a support, the upper surface of the support is fixedly connected with a mixing barrel, the upper surface of the mixing barrel is provided with a top cover, the inside of the top cover is fixedly connected with a feeding hopper, and the outer wall of the feeding hopper is slidably connected with a sealing cover. A mixing assembly used for mixing feed is installed on the upper surface of the top cover, a quantifying assembly used for quantitative discharging is installed on the lower surface of the mixing barrel, a culture pond is arranged on the lower surface of the support, and a drainage plate is fixedly connected to the bottom of the inner wall of the culture pond. According to the automatic feeding device, manpower and cost are saved through automatic constant-speed and uniform-amount feeding, the motor drives the conical barrel and the arc-shaped plate to rotate, the domestication rate is increased, fish growth is promoted, the feeding amount is accurately adjusted according to the fish feeding condition, the valve is used for quantification, the threaded sleeve can replace feed with different block diameters, practicability is improved, and waste is reduced.

The invention discloses a sepiella maindroni stepped wild domestication device which comprises a plurality of net cages arranged from inside to outside, each net cage is provided with a plurality of meshes, and the meshes in different net cages are gradually increased from inside to outside in a stepped manner; a plurality of suspension type fish reefs are arranged in the net cage, each suspension type fish reef comprises a plurality of single bodies horizontally installed on the floating rope, and each single body is a shell with a plurality of small holes formed in the shell wall. An area which is flat in terrain, relatively gentle in water flow, rich in plankton and about 10 m in water depth is selected near a sea area to be released as a domestication field, the device is put into the domestication field, and in-situ wild domestication is carried out on sepiella maindroni larvae; and the predation capability, the capability of avoiding enemies and the capability of coping with environmental stress after entering a natural sea area are improved, so that the enhancement and release effects are improved.

The invention relates to a mycorrhizal cultivation method for improving the stress resistance of pinus khasys seedlings, and belongs to the technical field of agricultural planting, the cultivation method comprises the following steps: (1) compounding fibrous belly fungi, bolete and russule virens into a microbial inoculum, and culturing to prepare microcapsules; (2) taking turf, vermiculite, humus soil and perlite as substrates, and adding a microcystis agent and a water-retaining agent; (3) the seeds are disinfected and sown, and seedlings are domesticated through light intensity gradient, water stress and temperature fluctuation; 4, during transplanting, abscisic acid is used for treating root systems, organic fertilizer is pre-filled, and mycorrhizal activation promoting liquid is perfused, through the synergistic effect of composite mycorrhizal fungi and stress domestication, the stress resistance, the survival rate and the afforestation adaptability of pinus khasys seedlings are remarkably improved.

The invention discloses a microbial remediation method and a microbial remediation system. The microbial remediation method comprises the following steps: collecting samples from a contaminated site, carrying out enrichment culture, screening and domestication to obtain various microbial strains, preparing a composite microbial agent according to a specific ratio, carrying out carrier immobilization treatment to form immobilized microbial particles, applying the immobilized microbial particles to soil in the modes of strip application, hole application and the like according to the contamination condition, or filling a suspended filler and putting the suspended filler into a water body. And environmental parameters are monitored in real time, and accurate regulation and control are performed to promote microorganisms to degrade pollutants. The microbial remediation system covers microbial culture and microbial agent preparation, putting and distribution, monitoring and regulation modules and the like, and automation and intelligentization of the whole process are achieved. The method is high in remediation efficiency, high in adaptability, low in cost, environmentally friendly and capable of effectively solving the problem of soil and water pollution.

The invention relates to the technical field of fry domestication, in particular to domestication bait and domestication method for mandarin fish fries. According to the specific technical scheme, the domestication bait for the mandarin fish fries comprises live crucian carp fries and silver carp fries, half-dead crucian carp fries and half-dead silver carp fries, mandarin fish powder, a mixture of the dead crucian carp fries and the dead silver carp fries and mandarin fish feed 0 #; a phagostimulant is added into the powder, and the phagostimulant is one of squid paste, betaine and hermetia illucens pulp. According to the three phagostimulants disclosed by the invention, the feeding domestication efficiency can be improved, the squid paste has the most obvious effect in the aspect of promoting the early growth performance and is excellent in related physiological metabolism indexes, and the betaine relieves the liver metabolism burden in the mandarin fish domestication process.

The invention discloses a method for establishing a medicinal plant litsea cubeba tissue culture seedling system. Aiming at the problems of difficulty in explant pollution and browning control, low proliferation and rooting efficiency, insufficient transplanting and domesticating survival rate and the like in establishment of a litsea cubeba tissue culture system in the prior art, the invention provides the establishment method of the medicinal plant litsea cubeba tissue culture seedling system, the germination rate of explants is 90% or above, and the transplanting survival rate in 30 days reaches 90.6%.

The invention aims to provide a morchella esculenta breeding method, an obtained morchella esculenta strain and application, the morchella esculenta is named as Morchella sextella NYD1, the taxonomic name of the morchella esculenta is Morchella sextella NYD1, the morchella esculenta is preserved in Guangdong Microbial Culture Collection Center, the preservation number of the morchella esculenta is GDMCC No: 66082, and the preservation date of the morchella esculenta is April 21, 2025. The morchella strain is further utilized as a mother strain to carry out domestication and cultivation, the morchella with the fruiting yield up to 400 kg / mu or above, excellent agronomic characters and rich nutritional ingredients such as crude protein, total sugar, iron, zinc and calcium is finally obtained, the content of the crude protein is not lower than 23 g / 100 g, the content of the total sugar is not lower than 55 g / 100 g, the content of the iron is not lower than 240 mg / kg, the content of the zinc is not lower than 70 mg / kg, and the content of the calcium is not lower than 76 mg / kg.

The utility model discloses a mandarin fish domestication flushing wave making device which comprises a water inlet pipeline used for achieving water inlet and stretching into a culture pond, a wave making pipeline is arranged at the end, away from the water inlet end, of the water inlet pipeline, and a water inlet fixing assembly used for fixing the position of the water inlet pipeline is arranged on the water inlet pipeline. A wave making supporting assembly used for fixing the position of the wave making pipeline is arranged on the wave making pipeline; the wave making pipeline comprises a wave making pipe corresponding to the culture pond, a plurality of water spraying holes are formed in the wave making pipe side by side at intervals, connecting pieces used for connecting the wave making pipe with other pipelines are arranged at the two ends of the wave making pipe, and the wave making pipe can change the directions of the water spraying holes in the connecting pieces. The direction of the water spraying holes in the wave making pipe can be changed through the connecting piece, and the wave making size, flow speed, frequency and direction can be changed through the variable frequency pump and the flow adjusting valve together, so that the mandarin fish wave making device meets the feeding characteristic requirements of mandarin fish in different growth stages.

The invention provides a sandy soil root domestication layered structure improvement method which comprises the steps that a surface guide layer, a channel induction layer and a deep resource layer are sequentially arranged on the earth surface from top to bottom, in-situ sandy soil is buried in the surface guide layer, a loose channel is constructed in the channel induction layer, and the loose channel is communicated with the deep resource layer; the deep resource layer comprises a water locking substance, a water retention substance, an adsorption substance and a supporting substance; when it is detected that the EC value of the joint of the surface guide layer and the channel induction layer is larger than the preset multiple of the EC value of the joint of the channel induction layer and the deep resource layer, a drip irrigation system is started to conduct drip irrigation on the surface guide layer according to the preset water pressure and water flow so as to guide nutrients in the surface guide layer to be collected towards the deep resource layer; according to the method, replacement of soil dressing is not needed, deep pricking and field planting of plant roots are guided through water and fertilizer stress and nutrition induction mechanisms, the drought resistance and stress resistance of plants are improved, the quality of crop products is optimized, a stable water and fertilizer environment is created for growth of plants in sandy soil, and the economical efficiency and effectiveness of the improvement technology are improved.

The invention relates to marine fish breeding, and particularly discloses an indoor full-artificial breeding method for black epinephelus, which specifically comprises the key technical links of nutrient enrichment of parent fish, hormone induced spawning, artificial insemination and fertilized egg incubation, indoor breeding of fry, compound feed domestication and the like. By controlling water temperature, illumination, salinity and nutritional conditions, synchronous maturation and efficient ovulation of parent gonads of the epinephelus nigatosus are achieved, the fertilization rate reaches 95% or above, and the hatching rate exceeds 85%. According to the method, the whole-process artificial controllable breeding of the epinephelus nigatosus from the parent fish to the fry is realized, a new variety is provided for the epinephelus culture industry, and a feasible technical approach is also provided for the artificial breeding and improved variety breeding of the wild epinephelus nigatosus in the island reef of the South China Sea.

The invention belongs to the technical field of mandarin fish culture, and relates to a mandarin fish domestication survival rate prediction method and system based on ecological simulation. Establishing a quantitative relation between the ecological factors and the sample mandarin fish survival rate change slope based on the quantitative relation so as to form an evaluation model; a historical survival rate curve is generated, and the risk state is determined by comparing the current sample mandarin fish survival rate change slope with the slope of the historical survival rate curve in the corresponding time section; calculating a change path of the ecological factors in a future time period based on the evaluation model, generating a predicted survival rate curve, and outputting an analysis report containing a risk state judgment result; according to the method, a complex domestication process is converted into a quantifiable data model, and early-stage accurate early warning of the risk is realized through dynamic analysis of the change trend of the survival rate.

A preparation method of an ionic rare earth leaching agent includes the following steps: (1) domestication microorganisms with rare earth activated mineral powder culture medium to obtain a microbial suspension; (2) amplifying and culturing the microbial suspension and additives to obtain the amplified culture medium; and (3) mixing the modified sesbania gum with the amplified culture medium to obtain the ionic rare earth leaching agent. The activated mineral powder is the active metal-containing mineral powder in nature, which has excellent cation exchange function after activation, and the activated mineral powder and ionic rare earth mineral powder are used as the medium components to domesticate microorganisms, so that microorganisms can survive in the above-mentioned ionic solution and improve the leaching rate of synergistic leaching ionic rare earth.

The invention provides a method for preparing rare ginsenoside through conversion of kombucha, the rare ginsenoside and application, and belongs to the technical field of biological medicine. A symbiotic system is formed by multiple bacteria such as acetic acid bacteria, gluconacetobacter, foal bacillus and the like and fungi, after the symbiotic system is domesticated, the American ginseng stem and leaf extract is fermented through multi-bacteria synergistic catalysis, the rare ginsenoside can be efficiently prepared, the highest conversion rate of Rb3 and Rd can reach about 90%, and the yield of Rb3 and Rd can reach about 90%. Meanwhile, the contents of rare ginsenosides Rg3 (20S), Rg5, Rh2 (20R) and Rh2 (20S) are remarkably increased and are increased by about 15 times at most, and efficient conversion of various ginsenosides is achieved.

The invention is suitable for the technical field of lucid ganoderma planting, and provides a wild lucid ganoderma leukemia domestication planting method which comprises the following steps: S1, strain preparation: collecting wild lucid ganoderma leukemia sporocarp, performing surface disinfection, cutting tissue blocks at the junction of pileus and stipe, inoculating the tissue blocks to an improved PDA mother strain culture medium, and culturing at 22-24 DEG C in a dark place for 15-20 days to obtain a purified mother strain; the stock culture medium is prepared from, by mass, 78% of oak sawdust, 18% of bran, 3% of soybean meal powder and 1% of gypsum powder, the water content is 60 + / -2%, the pH is 5.8-6.2, sterilization is conducted for 2 hours at the temperature of 121 DEG C, and culture is conducted for 25-30 days in the dark environment at the temperature of 23-25 DEG C and the humidity of 65 + / -5%. According to the domestication planting method for the wild ganoderma leucocontextum, the biological characteristics of the wild ganoderma leucocontextum are matched, breakthrough is achieved in the three aspects of a strain matrix, environment regulation and control and a drying process, the fundamental obstacle of artificial domestication is solved, and a core technical support is provided for sustainable development of valuable and rare medicinal fungi.

The invention relates to the field of microbial technology and ecological restoration of saline-alkali soil, in particular to plant growth-promoting paenibacillus for saline-alkali soil and application of the plant growth-promoting paenibacillus. The microbial inoculum comprises paenibacillus sp. XJ-1, P.megatherium Y-39, Endophytic glutamicum G-30 and Enterobacter C-29, and is prepared by mixing the paenibacillus sp. XJ-1, the P.megatherium Y-39, the Endophytic glutamicum G-30 and the Enterobacter C-29 according to a ratio of 1: 1: 1: 1: 1 and carrying out co-culture domestication, function synergistic induction and adaptability synchronous reinforcement. The viable count of the microbial agent is larger than or equal to 1 * 10 cfu / mL, the microbial agent can tolerate 15% NaCl, the pH value of the microbial agent is 9.5, the pH value of soil can be remarkably reduced through root irrigation or seed dressing application, the soil enzyme activity and the crop biomass can be improved, and the microbial agent is suitable for saline-alkali soil crops such as oilseed rape and wheat, has the advantages of being high in stress tolerance, synergistic in function, safe in ecology and the like and has wide application prospects.

The invention relates to the technical field of soil improvement and microorganisms, and discloses a preparation process of a porous activated carbon soil conditioner loaded with microbial flora, which comprises the following steps: firstly, performing high-temperature activation and acid-base modification on a biomass raw material to prepare a porous activated carbon carrier, and dipping in a nutrient solution to preset a slow-release nutrient substrate; secondly, adopting a step-by-step sequential loading process, firstly loading the bacillus subtilis subjected to stress resistance domestication on a carrier through electrostatic adsorption, and performing activation colonization culture to form a stable pioneer biological membrane; then, on the basis of the biological membrane, phosphate solubilizing bacteria, potassium solubilizing bacteria and antagonistic bacteria are loaded in sequence; finally, the loaded composite carrier is subjected to microcapsule embedding and low-temperature drying treatment, and the porous activated carbon soil conditioner is obtained. According to the modifier prepared by the invention, through ordered loading and multiple protection, the problem of strain competition is solved, the high survival rate of flora is ensured, and the improvement effect is stable and long-acting.

The invention discloses a biochar-based microbial soil conditioner and a preparation method thereof, and relates to the technical field of soil improvement. The preparation method comprises the following steps: firstly, carrying out alkali-acid treatment and segmented pyrolysis on raw materials such as wood chips to obtain primary biochar, and then carrying out chemical activation, nano material modification and plasma treatment to obtain the composite modified biochar. Meanwhile, probiotics such as bacillus subtilis and the like are subjected to activation, expanding culture and saline-alkali domestication to obtain directional domestication bacterial liquid, and the directional domestication bacterial liquid is solidified into microspheres by adopting a composite embedding carrier. And finally, mixing the composite modified biochar, the composite embedded microspheres, potassium humate and other auxiliary materials, drying, and sieving to obtain the soil conditioner. The soil conditioner disclosed by the invention can realize an improvement effect on saline-alkali soil and reduce the total salt content; meanwhile, directionally domesticated microorganisms secrete organic acid to neutralize alkaline components, the pH of the soil is adjusted to the range suitable for crop growth, and salt and alkali stress is relieved.

The invention discloses a staged regulation and control sulfur cycle enhanced denitrification microbial community construction method, and belongs to the technical field of environmental biologication.The method comprises the three stages that in the first stage, low-carbon-nitrogen-ratio domestication culture is conducted, the carbon-nitrogen ratio is 1.5-2.5: 1, and domestication is conducted for 50-70 days; in the second stage, electron donor-acceptor ratio regulation culture is carried out, the / molar ratio is (4-6): 1, and culture is carried out for 15-25 days; in the third stage, pulse type sulfate is fed for enhanced culture, the single-time feeding concentration is 200-500 mg / L, culture is conducted for 8-15 days, real-time monitoring is conducted through the fluorescence in-situ hybridization technology, construction is completed when the abundance of sulfur autotrophic denitrifying bacteria reaches 28% or above, and when the microflora constructed through the method is used for treating low-carbon-nitrogen-ratio industrial wastewater, the sulfur conversion rate is 1.2-2.0 kg / (m.d), the system stability is good, and the application range is wide. And the impact load resistance is high.

A compartment ditch three-dimensional frog breeding and feeding device relates to the technical field of ecological breeding and comprises a feeding table arranged on a compartment ditch, the feeding table comprises an elastic net used for feeding food and a supporting structure arranged on the edge of the elastic net, and the supporting structure is erected on the upper edge of the compartment ditch. The mesh size of the elastic net allows fine particles in the food to fall into the ditch below the elastic net. The cage ditch three-dimensional frog feeding device is suitable for cage ditch three-dimensional frog feeding, centralized feeding is carried out by arranging the feeding table above the cage ditch, and the food feeding efficiency is improved. The device has the innovative functions of adapting to field water depth management in different growth periods under ditch type rice cultivation, improving the feeding domestication efficiency, trapping field pests as frog bait and the like. According to the rice and frog symbiosis method, the mode that in traditional rice and frog symbiosis, feeding is conducted on the periphery of a farmland is improved, land resources are saved, multi-layer utilization of land is innovatively achieved, the agricultural output value is increased, and the requirement for the proportion of the crop planting area can be met.

The invention discloses application of an SNP (Single Nucleotide Polymorphism) molecular marker related to the character of siniperca chuatsi in feeding artificial compound feed, and belongs to the field of biotechnology and aquatic animal genetic breeding. The SNP molecular marker is located at the 29390304 site from the 5'end on the 10 # chromosome of the siniperca chuatsi genome, is A or T, and is easy to domesticate when the genotype is AA; or the SNP molecular marker is located at the 1218488 site from the 5'end on the 20 # chromosome of the siniperca chuatsi genome, and is easy to domesticate when being the genotype of T or A or TT. The SNP molecular marker is closely related to the artificial compound feed intake capacity of the siniperca chuatsi, so that the feeding domestication potential of the siniperca chuatsi can be predicted in an early stage through the SNP molecular marker, or molecular marker-assisted breeding is performed through the SNP molecular marker, the proportion of individuals easy to domesticate in a group is increased, the breeding cost is remarkably reduced, and the breeding efficiency is improved. The economic benefit is improved.

The invention belongs to the technical field of environmental protection, and relates to screening, domestication and application of focused polyacrylamide (PAM) for efficiently degrading fungi. The strain (# 12) is preserved in CGMCC (China General Microbiological Culture Collection Center) and is trichoderma asperellum, and the similarity between the ITS sequence of the strain and the gene reaches 99%. Aiming at soil hardening and acrylamide pollution caused by difficult degradation of non-ionic PAM in bauxite slime, the inventor adopts a gradient concentration screening and multi-stage domestication process. The method comprises the following steps: carrying out enrichment culture on slurry containing PAM, carrying out gradient screening in a basic solution with a specific concentration, separating and purifying to obtain an optimal strain, and carrying out continuous passage domestication through a six-stage culture medium, so that the degradation rate is increased by 23.45%. Under optimized conditions, the PAM degradation rate of the strain reaches 64.18%, the viscosity is reduced by 88.03%, degradation is achieved through physical destruction and enzymatic hydrolysis, and no acrylamide monomer is detected. The strain has activity at the temperature of 25-45 DEG C and the pH value of 4-8, is suitable for bioremediation of PAM-containing slime, alleviation of soil hardening and elimination of risks of toxic and side products, reveals the efficient degradation characteristic of trichoderma fungi to nonionic PAM for the first time, and assists in recycling of mining wastes.

The invention relates to a biomass energy utilization and resource utilization technology, and aims to provide a method for improving the carbon sequestration capability of nannochloropsis oculata by domesticating and regulating a key enzyme with high ammonium salt. The method comprises the following steps: carrying out nitrogen salt gradient domestication on nannochloropsis oculata cells, and regulating and controlling the expression quantity of five key enzymes, namely H < + > transport ATP enzyme, glutamine synthetase, asparagine synthetase, carbonic anhydrase and ribulose-1, 5-diphosphate carboxylase / oxygenase, in the nannochloropsis oculata cells; the domesticated and cultured nannochloropsis oculata can independently use coked ammonium sulfate as a nitrogen source required by growth; the domesticated algal strains are subjected to transfer culture, and verification and screening are carried out aiming at the capability of fixing CO2 in flue gas and the prevention of over-acidification of the culture medium. According to the method, the utilization of coked ammonium sulfate by the nannochloropsis oculata can be enhanced without causing over-acidification of the culture medium, the microalgae culture cost can be effectively saved, and the flue gas CO2 immobilization capability of the nannochloropsis oculata is improved; a new thought is provided for large-scale breeding and cultivation by using coked ammonium sulfate and improvement of economic efficiency.