243 results about "Domestication" patented technology

The invention discloses a biochar-based microbial soil conditioner and a preparation method thereof, and relates to the technical field of soil improvement. The preparation method comprises the following steps: firstly, carrying out alkali-acid treatment and segmented pyrolysis on raw materials such as wood chips to obtain primary biochar, and then carrying out chemical activation, nano material modification and plasma treatment to obtain the composite modified biochar. Meanwhile, probiotics such as bacillus subtilis and the like are subjected to activation, expanding culture and saline-alkali domestication to obtain directional domestication bacterial liquid, and the directional domestication bacterial liquid is solidified into microspheres by adopting a composite embedding carrier. And finally, mixing the composite modified biochar, the composite embedded microspheres, potassium humate and other auxiliary materials, drying, and sieving to obtain the soil conditioner. The soil conditioner disclosed by the invention can realize an improvement effect on saline-alkali soil and reduce the total salt content; meanwhile, directionally domesticated microorganisms secrete organic acid to neutralize alkaline components, the pH of the soil is adjusted to the range suitable for crop growth, and salt and alkali stress is relieved.

The invention discloses a staged regulation and control sulfur cycle enhanced denitrification microbial community construction method, and belongs to the technical field of environmental biologication.The method comprises the three stages that in the first stage, low-carbon-nitrogen-ratio domestication culture is conducted, the carbon-nitrogen ratio is 1.5-2.5: 1, and domestication is conducted for 50-70 days; in the second stage, electron donor-acceptor ratio regulation culture is carried out, the / molar ratio is (4-6): 1, and culture is carried out for 15-25 days; in the third stage, pulse type sulfate is fed for enhanced culture, the single-time feeding concentration is 200-500 mg / L, culture is conducted for 8-15 days, real-time monitoring is conducted through the fluorescence in-situ hybridization technology, construction is completed when the abundance of sulfur autotrophic denitrifying bacteria reaches 28% or above, and when the microflora constructed through the method is used for treating low-carbon-nitrogen-ratio industrial wastewater, the sulfur conversion rate is 1.2-2.0 kg / (m.d), the system stability is good, and the application range is wide. And the impact load resistance is high.

The invention discloses application of an SNP (Single Nucleotide Polymorphism) molecular marker related to the character of siniperca chuatsi in feeding artificial compound feed, and belongs to the field of biotechnology and aquatic animal genetic breeding. The SNP molecular marker is located at the 29390304 site from the 5'end on the 10 # chromosome of the siniperca chuatsi genome, is A or T, and is easy to domesticate when the genotype is AA; or the SNP molecular marker is located at the 1218488 site from the 5'end on the 20 # chromosome of the siniperca chuatsi genome, and is easy to domesticate when being the genotype of T or A or TT. The SNP molecular marker is closely related to the artificial compound feed intake capacity of the siniperca chuatsi, so that the feeding domestication potential of the siniperca chuatsi can be predicted in an early stage through the SNP molecular marker, or molecular marker-assisted breeding is performed through the SNP molecular marker, the proportion of individuals easy to domesticate in a group is increased, the breeding cost is remarkably reduced, and the breeding efficiency is improved. The economic benefit is improved.

The application discloses a mandarin fish domestication pool, which comprises a pool body, wherein an oxygenation device, a water inlet and a water outlet are arranged in the pool body, a bait fish breeding frame is arranged below the water inlet, and a feeding device is arranged on the side of the bait fish breeding frame; the feeding device comprises a feeding pipe, a bait temporary storage tank arranged on the upper end of the feeding pipe, a feeding hopper arranged on the upper end of the bait temporary storage tank, cathode ring pieces and anode ring pieces arranged in the feeding pipe, and a pulse generator and a pulse power amplifier connected through wires. The domestication pool is improved, so that live fish feeding, live fish and dead fish or feed proportionate feeding are facilitated, and switching and adjustment are facilitated, thereby improving the convenience and success rate of domestication.

The invention provides a breeding method suitable for breeding litopenaeus vannamei in saline-alkali soil, and relates to the technical field of aquaculture. According to the breeding method suitable for breeding the litopenaeus vannamei in the saline-alkali soil, the proportion of Ca < 2 + > to Mg < 2 + > to K < + > in a water body is optimized to be 2.8: 1: 0.3 by customizing a compound ion regulation and control solution, SO4 < 2-> / Cl <-> is controlled to be 0.14-0.8, and the pH is stabilized to be 8.2-8.8 in combination with a citric acid-sodium citrate buffer system; a slow-medium-fast segmented domestication strategy is adopted to adapt to different development stages of the fries; ethanol clostridium protein (CAP) is added into the feed to be combined with compound probiotics, so that immune metabolism regulation and control are enhanced. Experiments prove that according to the method, the survival rate of the litopenaeus vannamei seedlings reaches 86.3% + / -2.1%, the culture period is shortened by 12-15 days, the hepatopancreas SOD activity is improved by 42.6%, the vibrio inhibition rate reaches 78.5%, and the method is remarkably superior to the prior art. The method does not need large-scale pond transformation, adapts to different types of saline-alkali soil water bodies, realizes efficient green culture, and has important application value.

The application discloses a kind of gradient low temperature domestication, constant low temperature storage method of acrosternum hilare adult, comprising the following steps: (1) 10-15 day old acrosternum hilare adult is placed into storage box, the substrate and armyworm are loaded in the storage box;(2) the storage box is placed into artificial climate chamber and gradient low temperature domestication is carried out, and the gradient low temperature domestication program is: 26 DEG C →20 DEG C domestication 8h →15 DEG C domestication 8h →10 DEG C domestication 8h →6 DEG C domestication 8h →4 DEG C domestication 8h;(3) after domestication, acrosternum hilare adult is placed at 4 DEG C and refrigerated, and refrigeration time is 40-60 days.The application can guarantee normal fecundity and egg hatch rate, and better offspring development and offspring survival rate in its life period by placing acrosternum hilare adult into storage box loaded with substrate and armyworm and carrying out gradient low temperature domestication, and then placing it at 4 DEG C and refrigerating for 40-60 days.

The invention provides a fry adaptive domestication system and method based on multidirectional flow impact exercise, and relates to the technical field of fishery breeding, the system comprises three-section domestication pool bodies, the domestication pool bodies are detachably connected through flange plates, the bottom of each domestication pool body is fixedly connected with a supporting column, the upper portion of each supporting column is fixedly connected with a supporting seat, and the supporting seat is fixedly connected with the bottom of the corresponding domestication pool body. A motor is arranged on the upper portion of the supporting seat, a third bevel gear is fixed to the output end of the motor, and a connecting assembly facing the side wall of the domestication pool body in the radial direction is meshed with the third bevel gear. The invention provides a multi-directional flow impact training structure, which enables the fry to swim back and forth, turn and avoid and the like in radial, annular and spiral flow, the caudal peduncle muscle fiber density is improved by 20-30%, the swimming speed is improved by 40% compared with that of the traditional domesticated fry, the anti-countercurrent ability intensified training in the three-dimensional space of the fry is realized, and the endurance, explosive power and turning flexibility of the fry are improved in a balanced manner.

The present application belongs to the technical field of plant tissue culture, and particularly relates to a high-efficiency rooting and seedling raising method for plant tissue culture seedlings, S1 trimming and disinfection of S2 induction liquid immersion, S3 composite substrate implantation and gradient environment regulation, S4 substrate transplanting and planting; the core is to use modified magnetic biochar composite substrate loaded with FeO nanoparticles, cooperate with the synergistic effect of microencapsulated rooting hormone and melatonin, realize in-situ integrated seedling raising through composite spectrum containing UV-A, CO pulse supply, gradient humidity and PEG osmotic pressure domestication. The present application effectively inhibits basal browning, shortens the seedling raising period, improves the rooting quality and transplanting survival rate, overcomes the pollution prejudice of magnetic nanoparticles, is suitable for woody and herbaceous plants, and can be applied to plant tissue culture seedling production on a large scale.

The invention relates to a microbial agent for degrading COD (Chemical Oxygen Demand) in DBP (Dibutyl Phthalate) wastewater as well as a preparation process and application of the microbial agent. The microbial agent is prepared from the following active components in parts by weight: 88 to 91 parts of pseudomonas, 5.2 to 5.6 parts of enterobacter and 0.05 to 0.11 part of alcaligenes. According to the application, a plurality of microbial strains are formed in the microbial liquid which is subjected to mixed domestication culture of the DBP wastewater, the anaerobic sludge, the hydrolytic acidification bacteria and the nutritive salt, and a plurality of microbial strains in the microbial agent which is obtained by mixing the microbial liquid after bacteria screening and expanding culture have a synergistic effect, so that the microbial agent has the performance of efficiently degrading COD in the DBP wastewater in an environment-friendly manner.

The application discloses a wild hundred-petal grass domestication cultivation method and particularly relates to the field of wild plant cultivation technology, and comprises the following steps: step one, wild resource investigation and conservation, resource investigation is conducted on wild populations of hundred-petal grass, accompanying plants, original habitat types, soil conditions, illumination conditions, humidity conditions and distribution characteristics are recorded, germplasm resources of different populations are synchronously collected, including seeds and rhizomes, and the collected samples are numbered, stored in different zones and registered in traits, a seedling test area and a germplasm resource basic file are established, and basis is provided for subsequent directional domestication. The application improves seed germination rate and seedling uniformity, also improves transplanting survival rate and field adaptability, realizes wild resource protection and sustainable utilization, constructs an ecological planting mode suitable for local climate, improves stability and controllability of medicinal material quality, reduces chemical input and meets green development requirements.

The application discloses a tissue culture propagation method of eriochloa contracta. The creeping stem section of the eriochloa contracta is collected, washed, and then sterilized and dried; the sterilized and treated stem section is inoculated into a bud induction medium of MS+1-1.5 mg / L 6-BA+0.1-0.5 mg / L NAA+30 mg / L sucrose+6.5 mg / L agar to perform bud induction, and after 15-20 days of culture, the adventitious bud seedling is obtained; the adventitious bud of about 1-1.5 cm induced in the primary culture medium is inoculated into a proliferation culture medium to perform subculture, and the next step can be divided into single bud expansion or direct transplanting domestication. The two-step method only needs primary culture-proliferation and rooting to be simultaneously performed, and the transplanting survival rate is high.

The invention discloses a growth-promoting methylobacterium R5018 strain and an application of the growth-promoting methylobacterium R5018 strain. The strain R5018 is obtained by separation from a rice phyllospheric microflora and methanol domestication and screening, and is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M20252544. The rice growth-promoting microbial inoculum prepared from the strain can be stably colonized at phyllosphere and promote the growth of overground and underground parts of rice through seed soaking, foliage spraying or combined application of the seed soaking and the foliage spraying, and the ear number, the grain number and the yield are remarkably increased. Compared with the prior art, the strain disclosed by the invention is derived from a rice phyllospheric bacterial community, is high in adaptability, environment-friendly and efficient, provides a new microbial technical scheme for high-yield cultivation of rice, and has a wide popularization and application prospect.

The invention relates to the technical field of fertilizers, in particular to a fertilizer for relieving soil cadmium pollution and a preparation method thereof. The fertilizer for slowing down cadmium pollution of soil comprises the following raw materials in parts by weight: 30-50 parts of a modified charcoal-based carrier, 10-20 parts of a compound microbial agent, 15-25 parts of a nano mineral passivator, 5-10 parts of a chelated trace element compound fertilizer and 10-15 parts of potassium humate. Through a specific cascade domestication method, the compound bacterial system gradually adapts in a cadmium-containing environment and generates a synergistic metabolism mechanism, then the compound bacterial system is combined with the nano mineral modified by organic intercalation, and the nano mineral not only has strong adsorption capacity to cadmium, but also has strong adsorption capacity to cadmium. And the organic groups modified on the surface can also provide colonization sites and nutrition signals for the domesticated functional bacteria to form a triple barrier of'nano mineral physical passivation-functional bacteria biological resistance and control-metabolite complexing and curing ', so that the long-term effect and stress resistance of passivation are remarkably improved.

The invention discloses a culture and domestication method of a salt-tolerant heterotrophic nitrification and denitrification microbial agent, and belongs to the technical field of environmental microorganisms. Comprising the following steps: inoculating a heterotrophic nitrification culture medium with a halomonas seed solution, and culturing until a strain reaches a stable growth period to finish domestication. The Halomonas sp. SOB56 strain provided by the invention can be used for effectively removing nitrogen-containing substances in high-salinity wastewater such as mariculture wastewater and the like, and can be adapted to high-salinity wastewater with different salinity through domestication. The removal rates of the strain on ammonia nitrogen and nitrate nitrogen are 99.6% and 94.22% respectively within 36 hours when the strain enters a logarithmic phase under the condition that the temperature is 30 DEG C and the salinity is 5%. The biological nitrogen removal method belongs to the technical field of novel biological nitrogen removal, nitrate nitrogen and nitrite nitrogen are not generated in the ammonia nitrogen removal process, and the biological nitrogen removal method has a good application prospect in actual application of high-salinity wastewater such as mariculture wastewater.

The invention discloses an application and method of Tween 80 combined immobilization technology enhanced microorganisms in degradation of naphthalene pollution in water, the main body of the combined immobilization technology is SA / CS at Tween 80 hydrogel microspheres loaded with NM-1 flora, and the NM-1 flora is halotolerant bacteria obtained by enrichment and domestication from deep-sea sediments of a certain sea ditch. The SA / CS at Tween 80 microspheres are formed by crosslinking sodium alginate (SA), chitosan (CS) and Tween 80, hydroxyl functional groups of the Tween 80 and SA / CS are crosslinked to enhance the mechanical strength of the microspheres and regulate and control the pore structure to improve the mass transfer efficiency, hydrophobic-CH functional groups at the end positions of the Tween 80 strengthen the adsorption capacity to naphthalene, meanwhile, a co-crosslinking structure at the interface of the SA / CS at Tween 80 hydrogel microspheres forms a biochemical metabolic reaction buffer area, and the adsorption capacity to naphthalene is improved. The direct toxicity stress of the high-concentration naphthalene on the flora is effectively avoided, and the effective biological removal of the high-concentration naphthalene is realized. The SA / CS-coated Tween 80 hydrogel microspheres prepared by the invention can effectively maintain the efficient metabolic activity and degradation efficiency of the loaded NM-1 flora, and show excellent cycle stability and good engineering application potential.

The invention relates to the technical field of microbial fermentation, and particularly discloses a high-density expanding culture method of autotrophic nitrifying bacteria. The method comprises the following steps: screening low-temperature aquatic plants, and carrying out stepped pressurization domestication to obtain low-temperature-resistant and high-salt-resistant autotrophic nitrobacteria strains; a three-stage stepped expanding culture process is adopted, and a carrier filler with a high specific surface area is added in second-stage fermentation and third-stage fermentation; in the fermentation process, continuous collaborative fed-batch feeding, pH linkage regulation and control and dissolved oxygen precise linkage control of an inorganic carbon and nitrogen source are implemented. Through stress-resistant strain screening, carrier adding and precise process control, the density of the thalli in the fermentation liquor can be increased, the stress resistance of the thalli is improved, and the method is suitable for a more complex water body environment.

The invention belongs to the field of improvement of acid tolerance and high-density fermentation of probiotics, and particularly relates to a domestication method for improving acid tolerance and fermentation density of lactobacillus plantarum and an obtained strain. The invention provides a domestication method for improving acid tolerance and fermentation density of lactobacillus plantarum, which is characterized in that Lactiplantarum ZJ316 (CCTCC NO: M 208077) is used as a wild strain, the wild strain is domesticated, and the obtained domesticated strain ZJ316PV (Lactiplantarum ZJ316PV) has the preservation number of CGMCC NO.33082. The acclimatization method for improving acid tolerance and fermentation density of lactobacillus plantarum has the advantages that the acclimatization method comprises the following steps: taking Lactiplantarum ZJ316 (CCTCC NO: M 208077) as the wild strain; the acid tolerance of the domesticated strain ZJ316PV is superior to that of the wild strain ZJ316. The invention also provides a method for improving the PV fermentation density of the wild strain ZJ316 and the domesticated strain ZJ316.

This invention relates to the field of feed additive technology, specifically to a Bacillus subtilis composition for improving animal intestinal health and its preparation method. It comprises the following raw materials: Bacillus subtilis engineered bacterial powder, cinnamaldehyde microcapsules, proanthocyanidins B2, selenium-enriched yeast culture, glycoterpenes, trehalose, and a carrier. The core of this invention lies in using Bacillus subtilis EhrBS-9 (CGMCC No. 24947) as the strain, constructing a dual-function engineered bacterium with tolerance and transformation through gradient cinnamaldehyde domestication and cad gene integration. This, combined with the intestinal-targeted delivery of cinnamaldehyde microcapsules, barrier strengthening of proanthocyanidins B2, and the construction of a multidimensional anti-stress network of selenium-enriched yeast, glycoterpenes, and trehalose, forms a synergistic mechanism of gut microbiota regulation, immune enhancement, and stress buffering. This addresses the industry pain point of insufficient synergistic effects of existing feed additives in terms of high-efficiency antibacterial activity, safe immune regulation, root-cause anti-stress, and ecological safety.

The embodiment of the application discloses a kind of based on low salt domestication and partition feeding mandarin fish fry scale-up cultivation method.The method is by providing low salinity domestication pool, and mandarin fish fry is put into the low salinity domestication pool, simultaneously based on light-flow composite stress domestication food process, using partition feeding and intelligent bait conversion system to mandarin fish fry is converted food, after mandarin fish fry is completed conversion food, hierarchical water quality regulation is carried out and micro-ecological steady state is constructed, until mandarin fish fry is out of pool, and is transferred into fish culture, to solve mandarin fish fry conversion food difficult, survival rate is low, and disease frequently occurs etc.Industrialization pain point, for realizing mandarin fish large specification fry scale-up, standardization, green production provides complete technical scheme, with wide popularization prospect and significant economic, social, ecological benefits.

The invention discloses an acetate-resistant domesticated algal strain HpAc50 and an application thereof. The algal strain is preserved in the China Center for Type Culture Collection on January 12, 2026, and the preservation number is CCTCC M 202690. The haematococcus pluvialis strain is obtained by carrying out acetate-resistant domestication on haematococcus pluvialis and can be cultured under the condition that the acetic acid concentration is 0-10g / L, and the yield of astaxanthin is effectively increased. The invention specifically provides three application conditions: illumination of 0-5000lux and a full-nutrient culture medium; illumination is performed at 0-5000 lux, a nitrogen-phosphorus-deficient culture medium is adopted, and an exogenous stimulant is added According to the present invention, with the application of the strain, the cell density, the biomass and the astaxanthin accumulation can be significantly improved under the conditions of illumination of 5000-15000 lux and nitrogen and phosphorus deficiency culture medium, such that the strain has strong acetate tolerance and strong production performance compared with the wild type strain, and is suitable for the large-scale astaxanthin production.

The application provides a seven-day period factory cyclic Megalobrama amblycephala fry behavior domestication method and system, relates to the Megalobrama amblycephala breeding technical field, and includes: domesticating the fry into a domestication pond on the first day; starting vertical water flow impact before feeding fresh bait fish according to a preset first feeding frequency on the second day; feeding electric shock disabled live bait according to a preset second feeding frequency on the third day; feeding mixed live bait according to a preset third feeding frequency on the fourth day; feeding mosaic bait according to a preset fourth feeding frequency on the fifth day; feeding pure artificial compound feed using a composite feeding mode and starting water flow impact on the sixth day; feeding pure artificial compound feed on the seventh day, and screening domestication successful individuals based on behavior indexes after the period ends, so that the Megalobrama amblycephala is rapidly domesticated from feeding live bait to feeding artificial compound feed.

This invention discloses a method for the domestication, isolation, purification, and application of a low-temperature resistant straw-degrading bacterium, belonging to the fields of agricultural microbiology technology and waste resource utilization. The domestication method specifically includes sample preparation, straw pretreatment, culture medium preparation, and multiple rounds of low-temperature domestication culture. During this process, the straw weight loss rate is used as an indicator to detect the degradation efficiency of the bacterial community after each round of domestication. The obtained low-temperature resistant straw-degrading bacterium was deposited on April 27, 2026, at the China Center for Type Culture Collection (CCTCC), located at No. 299, Bayi Road, Wuchang District, Wuhan City, Hubei Province; the accession number is CCTCC NO: M2026814; the taxonomic name of the low-temperature resistant straw-degrading bacterium is *Brucea buddinga* X4-8. (Aureobasidium sp. X4-8) The domestication method of this invention actively enhances the low-temperature adaptability and degradation activity of microbial communities by combining exogenous amino acid induction with systematic cyclic domestication. Furthermore, this invention also discloses a method for isolating and purifying low-temperature resistant straw-degrading bacteria and its application.

The present application relates to the field of domestication of fish, and particularly relates to an artificial domestication method of Leptobotia rubipinnis. The method comprises the following steps: collecting and transporting wild parent fish, temporarily raising the wild parent fish, and domesticating the wild parent fish. The domestication process of the wild parent fish comprises the following steps: ① feeding biological bait, ② feeding biological bait and artificial compound feed, and ③ feeding artificial compound feed. The method has the advantages of simple operation process, and the use of the segmented temporary raising and domestication, gradual conversion from biological bait to artificial compound feed, and regulation of water flow rate during feeding, so that the death of the parent fish due to starvation is avoided, thereby improving the survival rate (more than 75%), the food conversion rate and the artificial domestication survival rate (≥68%) of the wild parent fish, and providing technical support and basic data accumulation for the artificial propagation, disease prevention and control and artificial propagation and release of Leptobotia rubipinnis.

The invention relates to an artificial domestication and cultivation method for Mongolian white mushroom based on micro-ecological simulation, and innovatively provides a micro-ecological simulation cultivation strategy aiming at the core technical bottlenecks that hyphae of Mongolian white mushroom grow extremely slowly, a conventional culture medium does not eat materials and fruiting is difficult. The method is characterized in that specific soil leaching liquor is prepared from mongolian white mushroom circle rhizosphere soil and added into a mother strain culture medium, and key microbial flora and growth factors for promoting hypha growth are successfully introduced; meanwhile, a special stock seed and a cultivation material which take fermented sheep manure and pasture root system granules as main materials are designed, and nutrients and physical structures of the original grassland ecology are accurately simulated. According to the method, the problem of hypha growth stagnation is thoroughly solved, the culture periods of mother seeds, stock seeds and cultivated seeds are remarkably shortened to 14 days, 22 days and 46 days from conventional 30 days or more, 40 days or more and 66 days or more, stable fruiting is successfully achieved, and the biotransformation rate is increased by 11.2% or more compared with that of a conventional method.

The application discloses a kind of whole growth period active cold resistance efficient cultivation methods of cabbage seedling, it is related to agricultural planting technical field, the specific steps of the method are as follows: selecting the growth of healthy cabbage seed mother plant, sprays 5-azacytidine and abscisic acid mixed solution, after seed ripening, air-drying is preserved;Seed disinfection is planted in seedling substrate hole tray, conventional management is until 2 leaf 1 heart period;The root group of seedling is placed in low temperature and suitable temperature two independent culture rooms and is carried out partition domestication;After domestication is completed, planting is planted in field, and water and fertilizer management and the prevention and cure of plant disease and insect pest until harvest;The application is improved low-temperature tolerance characteristics and stable genetic offspring by directional cold resistance induction of cabbage bolting period and seed standard treatment from germplasm source head;Root system partition difference temperature domestication is used, avoid root hair root tip damage, maintain normal water and fertilizer absorption function;And supporting link domestication, planting, whole cycle cultivation management system of management, significantly improve planting survival rate.

The application discloses a high-phosphorus hematite biological desphosphorization method based on micro-electric field stepwise domestication and nutrient slow-release regulation, and belongs to the technical field of mineral processing and bio-metallurgy. The biological desphosphorization method comprises the following steps: culturing Bacillus subtilis and Bacillus megaterium to the logarithmic growth phase to obtain active bacteria suspension of a combined bacterial flora; placing the active bacteria suspension in different high-phosphorus hematite slurries from low concentration to high concentration, and obtaining a dominant functional bacterial flora under the regulation of micro-electric field and nutrient slow release; culturing the dominant functional bacterial flora to the logarithmic phase, then adding pretreated high-phosphorus hematite powder for leaching, monitoring the pH value, and separating to obtain leaching solution I and leaching residue I; mixing the leaching residue I with a fungal leaching medium, inoculating a fungus Talaromyces for synergistic leaching, and separating to obtain leaching solution II and desphosphorized iron concentrate. The application successfully constructs an efficient, stable and environment-friendly functional bacterial flora + fungus synergistic system, and realizes efficient removal of phosphorus elements in high-phosphorus iron ore in a short period.

This invention discloses a highly acid-resistant Bacillus belye strain LYBV022-Ac R This invention relates to a method for screening and acclimating bacteria under progressive acid stress, and its application, belonging to the technical field of industrial microbial breeding and probiotic application. The disclosed high-acid-resistant Bacillus belyeis LYBV022-Ac... R By designing a pH gradient decreasing (6.5→3.0) acclimatization strategy and combining it with microplate rapid screening technology, a breakthrough improvement in acid resistance was achieved. After exposure to a simulated gastric acid environment (pH 3.0, containing pepsin) for 4 hours, the survival rate reached 48.52%, a 970-fold increase compared to the original strain (0.05%), while maintaining excellent growth performance and genetic stability. This invention also provides the application of this strain in the preparation of oral probiotic preparations for livestock and poultry, solving the common technical problem of insufficient potency in oral Bacillus preparations due to gastric acid damage.

The invention provides a method for screening and cultivating multi-substrate degrading bacteria and algae resistant to an extreme environment. The method comprises the following steps: sampling at least two habitats from an industrial wastewater pool, a compost fermentation heap and saline-alkali soil, and pretreating to obtain an initial bacteria and algae suspension; a composite carbon source culture medium containing cellulose, lignin and fat is adopted, a salinity, pH and temperature multi-dimensional gradient environment is set, and finally the culture medium is transferred to a more extreme culture medium for domestication; diluting the enriched bacteria and algae groups, injecting the diluted enriched bacteria and algae groups into a micro-fluidic chip containing a fluorescence labeled substrate, sorting single cells with the fluorescence decay rate of more than or equal to 80% by using a flow cytometer, and performing inoculated culture; extracting DNA for sequencing, and combining physiological and biochemical detection and product analysis to obtain potential bacteria and algae; and after compound mutagenesis, inoculating to a reactor to gradually increase the substrate concentration for domestication. According to the method disclosed by the invention, through an integrated technical chain of multi-source sampling pretreatment, carbon source gradient enrichment, high-throughput screening, multi-dimensional identification and directional breeding, high-activity stable bacteria and algae resources which are resistant to an extreme environment and can synchronously degrade various organic matters are rapidly obtained.

This utility model relates to the field of juvenile acclimatization pond technology, specifically a juvenile acclimatization pond for mandarin fish based on a recirculating aquaculture system. The pond includes a circular body with a fish inlet fixedly connected to the top of its side wall. This fish inlet is connected to the opening pond via a pipe. The advantages of this utility model are: the circular pond structure combined with a central drain outlet at the bottom allows for rapid and directional discharge of pollutants such as uneaten feed and feces, effectively reducing the entry of large particulate pollutants into the recirculating water treatment system and lowering energy consumption. The unique fish inlet design allows mandarin fish juveniles to be automatically transferred from the opening pond to the acclimatization pond via water flow guidance, avoiding mechanical damage and stress caused by manual harvesting and significantly reducing juvenile loss. The annular spray jet design simulates a natural feeding environment through regular water flow stimulation, successfully establishing a conditioned reflex feeding mechanism in the mandarin fish juveniles, increasing the acclimatization success rate to over 85%.

The invention discloses a fry domestication and cultivation device, and relates to the technical field of aquaculture equipment. The device comprises a fishpond groove, a support structure, a detachable net cage structure, a feeding structure and a shielding structure, a V-shaped sewage collecting structure composed of a slope boss is arranged at the bottom of the fishpond groove, and residual feed and excrement are discharged in a concentrated mode in cooperation with a sewage pump; an aerator is arranged in the sewage collection area to improve the dissolved oxygen level of bottom water; the inner wall of the tank is provided with a flushing nozzle capable of adjusting the spraying direction and flow and used for simulating a natural water flow environment; the top shielding structure can be adjusted in a sliding mode and is matched with the spotlight to achieve illumination control. The device is reasonable in structure, high in function integration level, capable of effectively improving the water quality environment, reducing the fry stress response and improving the feeding domestication success rate and survival rate and suitable for multi-stage fry domestication and cultivation.