691 results about "Domestication" patented technology

The invention provides a digital twinborn monitoring, regulating and controlling method for a copepod bait biological domestication process. The method comprises the following steps: establishing a micro-ecology and population dynamics coupled twinborn model, and defining a monitoring parameter and control object unified term system; collecting multi-source sensing data at an edge end, and performing clock synchronization, signal denoising and state estimation to obtain a micro-ecology, group and water body state joint estimation result; executing parameter online identification based on state estimation, and constructing a safety constraint set of ammonia nitrogen, dissolved oxygen, death rate, environment change rate and the like; the breeding efficiency improvement and the system stability balance are realized through a multi-objective optimization function; and generating a micro-ecological pulse and environment gradual change cooperative control action set according to a solving result, and when a set condition is met, outputting a domestication prescription and terminating the process. Intelligent, low-stress and high-stability regulation and control of a copepod domestication process are realized through digital twin modeling, rolling predictive control and a self-evolution updating mechanism.

The invention discloses a kitchen waste oil-based sophorolipid high-yield candida utilis strain and an application thereof. Belongs to the technical field of directional domestication breeding. According to the method, wild candida bombicola is used as an original strain, and is sequentially domesticated in a culture medium containing kitchen waste oil with the concentration gradient of 2%, 4%, 6%, 8% and 10% to obtain the S.bombicola x10. The invention further discloses an application of the domesticated strain in fermentation production of sophorolipid, after the strain is subjected to shake flask fermentation, the sophorolipid yield reaches 60.19 g / L and is improved by 16.22% compared with that of a wild strain, and the acid type sophorolipid yield is improved by 32.11%. When the concentration of the lactone sophorolipid generated by fermentation is 1.25 mg / mL, the growth of the bacillus subtilis is completely inhibited. The invention provides an excellent production strain for industrial production of sophorolipid, and has good theoretical research significance and practical application value.

The invention provides an inoculum domestication method for relieving acid or ammonia nitrogen inhibition of an anaerobic fermentation system, which comprises the following steps of: carrying out constant-temperature anaerobic fermentation culture at medium temperature (30-35 DEG C) or high temperature (50-55 DEG C) until the gas yield is stable by taking livestock manure, activated sludge, industrial anaerobic digestion residues or mineralized refuse biogas fermentation liquor and the like as inoculums; the obtained inoculum is an inoculum which has relatively strong acid tolerance or ammonia nitrogen tolerance after domestication. The domesticated inoculum with good tolerance and the original untreated inoculum are mixed according to a certain proportion and then put into an anaerobic fermentation system, and wet fermentation (TS = 5-10% and total solid content) or dry fermentation (TS = 20-30%) is carried out, so that the pH value of the anaerobic fermentation system is recovered to a state suitable for growth of methanogens.

According to the microbial flora culture method for efficiently degrading the organic pollutants, three continuous culture stages of enrichment, domestication and optimization are adopted, parameters such as the temperature, the pH value, the rotating speed and the ventilatory capacity are accurately controlled in each stage, and growth of microorganisms with degradation capacity is promoted. Through multi-stage culture and domestication, the obtained flora has efficient degradation capability on organic pollutants such as petroleum hydrocarbons, pesticides and polycyclic aromatic hydrocarbons, and has strong adaptability to pollutants with different concentrations. The 16S rRNA gene is used for sequencing and identifying the flora, and composition and dominant strains are clear. The flora is prepared into a microbial inoculum, and the microbial inoculum is preserved by freeze drying or liquid nitrogen, so that the activity can be effectively maintained. The flora can be widely applied to the fields of polluted soil and water body remediation, industrial wastewater treatment and the like, is green and environment-friendly, is relatively low in cost, avoids secondary pollution, conforms to the concept of sustainable development, and has relatively high economic and environmental values.

The invention provides a high-salinity high-tidal-flat mangrove forest ecological restoration method. The high-salinity high-tidal-flat mangrove forest ecological restoration method comprises the following steps of S1, soil improvement, S2, seedling cultivation, S3, seedling planting, S4, seedling reseeding, S5, bank protection device arrangement and S6, pest control. The soil conditioner is used for improving the soil structure and reducing the soil salinity. Salt-resistant domestication is performed on the seedlings by gradually improving the salinity of irrigation water, so that the salt resistance of the seedlings is improved. Organic fertilizer, ferriporphyrin, abscisic acid and brassinolide are used for providing nutrients for seedlings, osmotic equilibrium in plants is adjusted, opening and closing of air holes are adjusted, plasticity of cell walls is adjusted, and therefore the salt resistance of the plants is enhanced. According to the method, the roots of seedlings are inoculated with the halorhodobryum terbach and the proteobacteria, the halorhodobryum terbach and the proteobacteria terbach form a symbiotic relationship with the roots of plants, a microbial community is formed, and the absorption efficiency of the plants to nutrients in soil is According to the method, the planting survival rate and the plant growth rate are improved through the multi-way overall synergistic effect, and then the biological diversity is improved.

The invention belongs to the technical field of molecular breeding of aquatic products, and particularly relates to an Indel molecular marker for identifying production places of finless eels and application thereof, which are particularly suitable for identifying finless eel strains in Jiangxi regions and non-Jiangxi regions. The Indel molecular marker for identifying the producing area of the ricefield eel is an Indel molecular marker B, and the Indel molecular marker B contains nucleotide sequences as shown in SEQ ID NO: 3 and SEQ ID NO: 4. The monopterus albus strains in Jiangxi regions and non-Jiangxi regions can be rapidly identified by utilizing the molecular marker, genetic identification can be rapidly realized, domestication breeding of wild germplasm resources is accelerated, and precise breeding is realized.

The invention relates to the technical field of fishery breeding, in particular to a step depth adjustable mandarin fish domestication breeding water temperature detection device and a domestication method.The step depth adjustable mandarin fish domestication breeding water temperature detection device comprises a mounting plate; a lifting assembly capable of driving a lifting piece arranged on the mounting plate to intermittently descend in the longitudinal direction is arranged on the mounting plate; triggering pieces are arranged on the mounting plate at equal intervals. A temperature measuring cylinder is arranged on the lifting part, the temperature measuring cylinder is communicated with a water sample extracting assembly on the lifting part, the water sample extracting assembly comprises a suction cylinder which is arranged on the lifting part and is communicated with the temperature measuring cylinder, a suction part is arranged in the suction cylinder, and the suction part is matched with a driving assembly on the lifting part and can suck a water sample into the temperature measuring cylinder; when the driving assembly is separated from the triggering piece and is quickly reset, the suction piece acts reversely, and the sample pumped into the temperature measuring cylinder is discharged; through cooperation of all the assemblies, culture personnel can conveniently and accurately obtain the temperatures of water bodies at different depths of a culture pond.

The invention discloses a salt-tolerant reed seedling and a cultivation method thereof. The method comprises the following steps: seed selection and pretreatment; performing exogenous induction treatment; performing germination culture; salt gradient domestication culture; salt tolerance screening, seedling hardening and transplanting. According to the invention, by combining exogenous induction and salt gradient domestication culture, the stress resistance of the plant is regulated, and the salt tolerance mechanism in the seed is activated, so that a foundation is laid for better adaptation and growth in a high-salt environment subsequently; the salt-tolerant reed seedlings induced and domesticated by the method are high in seedling rate, the survival rate of acclimatization and transplantation is 90% or above, the breeding cycle is shortened to be within 100 days, the cultivation cycle is short, and the method is suitable for large-scale popularization and application.

The invention belongs to the technical field of molecular breeding of aquatic products, particularly relates to an Indel molecular marker for identifying ricefield eel strains and application thereof, and is particularly suitable for identifying ricefield eel strains in Jiangxi regions and non-Jiangxi regions. The Indel molecular marker for identifying the ricefield eel strain is an Indel molecular marker A, and the Indel molecular marker A contains nucleotide sequences as shown in SEQ ID NO: 3 and SEQ ID NO: 4. The monopterus albus strains in Jiangxi regions and non-Jiangxi regions can be rapidly identified by utilizing the molecular marker, genetic identification can be rapidly realized, domestication breeding of wild germplasm resources is accelerated, and precise breeding is realized.

The invention belongs to the technical field of fish genetic identification, discloses a multi-site large yellow croaker wild domestication population identification system based on an agarose gel electrophoresis technology, and particularly discloses a group of InDel molecular markers for large yellow croaker wild and domestication population identification, and the InDel molecular markers comprise InDel11-5053756, InDel20-14004876, InDel3-28345321 and InDel21-17327392. The InDel molecular marker detection primer is used for amplification to obtain a PCR product, agarose gel can be used for detecting strip polymorphism, extra restriction enzyme digestion or complicated polyacrylamide gel electrophoresis operation is not needed, and identification of large yellow croaker wild and domesticated populations can be rapidly and accurately achieved.

The invention provides a feed domesticated siniperca chuatsi sperm low-temperature preservation and ultralow-temperature preservation method, which comprises the following steps: firstly, providing a siniperca chuatsi sperm low-temperature preservation diluent which comprises Hanks liquid, sodium chloride, soya bean lecithin, vitamin E, curcumin, melatonin and SOD (superoxide dismutase); and adding an anti-freezing protective agent into the low-temperature preservation diluent to obtain the ultralow-temperature preservation anti-freezing liquid for the siniperca chuatsi sperms. The siniperca chuatsi semen and the low-temperature preservation diluent are uniformly mixed, so that low-temperature preservation can be realized; the siniperca chuatsi semen is uniformly mixed with the cryopreservation anti-freezing liquid, so that cryopreservation can be realized. The siniperca chuatsi sperm low-temperature preservation diluent is preferably selected, low-temperature preservation of the siniperca chuatsi sperms can be achieved, ultralow-temperature preservation can be conducted after an anti-freezing protective agent is added, the persistence of the preservation time of the siniperca chuatsi sperms can be guaranteed, the protection effect on the sperms is good, and the activation rate and activity of the unfrozen sperms are high.

The utility model discloses a bletilla striata tissue culture seedling germination accelerating and rooting soaking device which comprises a fixing support, object carrying platforms and soaking mechanisms, the fixing support is of a rectangular frame structure, the upper portion of the fixing support is provided with a plurality of object carrying platforms, and the upper portions of the object carrying platforms are provided with a plurality of soaking mechanisms; the soaking mechanism comprises a chaining table, a connecting rod, a rectangular opening, a protection plate and seedling raising bottles, the chaining table is of a cylindrical cavity structure with the upper portion open, the connecting rod is arranged in the chaining table, the middle of the connecting rod is movably connected with the rectangular opening in the upper portion of the chaining table through a connecting shaft, and the protection plate is arranged at one end of the connecting rod; a lower pressing plate is arranged at the other end of the connecting rod; and the seedling raising bottles are arranged in the chaining tables. The domestication seedbed is used for solving the problem that in the moving process of an existing domestication seedbed, cultivation bottles are prone to rolling and falling off.

The invention provides a wild domestication system and method for yellow lip fish, and belongs to the technical field of germplasm resource restoration and maintenance of endangered fishes. The natural environment is simulated, and food sensitivity training, ecological inversion adaptability training, instantaneous cruising ability and stress adaptability training are applied to the yellow lip fish, so that the ingestion and hunger resistance of the released yellow lip fish are enhanced, the survival abilities of avoiding disaster events, reducing stress response and the like are improved, and the survival rate of the released yellow lip fish is increased. Therefore, the survival rate of the released yellow lip fish in a natural sea area is improved. By adopting the system and the technical method for simulating the natural environment, a very good proliferation effect is achieved on the release of the yellow lip fish, and the system and the technical method have a wide application prospect.

The invention relates to a key gene for duck meat and egg character differentiation and a method for excavating the key gene based on whole genome sequencing, the key gene is a duck SIK1 gene, and the nucleotide sequence of the key gene is shown as SEQ ID NO: 1. The key gene SIK1 influencing the differentiation and domestication process of laying ducks and meat ducks is found by using a method for analyzing the key gene in the domestication process of duck meat traits and egg traits through whole genome re-sequencing. The gene SIK1 plays a role in regulating glycometabolism and cell proliferation. The genome of the Jinding duck is assembled by using a whole genome re-sequencing technology for the first time and is compared with the genome of other duck species such as Beijing duck, another key gene SIK1 for meat and egg trait differentiation of ducks is excavated, molecular breeding of ducks is facilitated, and a new thought is provided for searching key genes for meat / egg duck trait differentiation in future.

The invention discloses a wetland system plastic pollution intelligent degradation system based on artificial intelligence driving, and relates to the field of ecological environment protection and artificial intelligence fusion. The problems that an existing wetland system lacks real-time monitoring and predicting capacity for the migration behavior of the micro-plastics, the combination of a microbiological degradation technology and the wetland system is not tight enough, and efficient degradation of the micro-plastics is difficult to achieve are solved. According to the system, an AI behavior path prediction model is adopted to predict a micro-plastic migration track and a micro-plastic distribution probability corresponding to the migration track, a structure optimization subsystem is utilized to optimize wetland structure parameters and filler distribution, and a directional domestication microorganism technology is combined to enhance the degradation capacity. The method is mainly applied to micro-plastic degradation of a wetland system.

The utility model relates to the technical field of aquaculture, and discloses a domestication device for artificial fish compound feed, which comprises a support, the upper surface of the support is fixedly connected with a mixing barrel, the upper surface of the mixing barrel is provided with a top cover, the inside of the top cover is fixedly connected with a feeding hopper, and the outer wall of the feeding hopper is slidably connected with a sealing cover. A mixing assembly used for mixing feed is installed on the upper surface of the top cover, a quantifying assembly used for quantitative discharging is installed on the lower surface of the mixing barrel, a culture pond is arranged on the lower surface of the support, and a drainage plate is fixedly connected to the bottom of the inner wall of the culture pond. According to the automatic feeding device, manpower and cost are saved through automatic constant-speed and uniform-amount feeding, the motor drives the conical barrel and the arc-shaped plate to rotate, the domestication rate is increased, fish growth is promoted, the feeding amount is accurately adjusted according to the fish feeding condition, the valve is used for quantification, the threaded sleeve can replace feed with different block diameters, practicability is improved, and waste is reduced.

The invention discloses a fermentation process and a preparation of lactobacillus reuteri L-01, and belongs to the field of microorganism preparation.After artificial domestication, bile salt tolerance of the lactobacillus reuteri L-01 is enhanced, the lactobacillus reuteri L-01 can be cultured in gastrointestinal fluid, and the survival rate of 98% or above can still be kept for a long time; the lactobacillus reuteri L-01 disclosed by the invention can play an excellent probiotic function, is beneficial to improving the balance of intestinal flora, has an inhibiting effect on pathogenic bacteria, and reduces the prevalence rate. Besides, after the components of the culture medium are optimized, the domesticated lactobacillus reuteri L-01 also has a certain anti-oxidation effect and is rich in selenium, so that the use value of the lactobacillus reuteri L-01 is increased. The lactobacillus reuteri L-01 fermentation broth is obtained, after a protective agent is added, the more excellent probiotic effect can be guaranteed, the stability of the lactobacillus reuteri L-01 in processing application and transportation storage is enhanced, and the lactobacillus reuteri L-01 fermentation broth has a certain slow-release effect and has a longer acting effect.

The invention belongs to the field of domestication and cultivation of edible mushrooms, and particularly relates to an agrocybe cylindracea strain and a domestication and cultivation method. The breeding method of the new agrocybe cylindracea strain Ganxi agrocybe cylindracea No.1 comprises the following steps: (1) activating and culturing an agrocybe cylindracea strain mother strain; (2) preparing a stock seed and a cultivar; (3) preparing a cultivation material bag; (4) culturing the cultivation bag; through sporocarp tissue separation, artificial domestication and systematic selection, an excellent new variety which can be applied to production is finally cultivated and can be used as an optimized variety for facility and factory cultivation of agrocybe cylindracea. Through domestication and cultivation, suitable growth conditions and cultivation characteristics of the 'Ganxi agrocybe cylindracea No.1' are defined, an efficient cultivation technology matched with the variety is established, and the 'Ganxi agrocybe cylindracea No.1' variety has great popularization and application potential.

The invention discloses a sepiella maindroni stepped wild domestication device which comprises a plurality of net cages arranged from inside to outside, each net cage is provided with a plurality of meshes, and the meshes in different net cages are gradually increased from inside to outside in a stepped manner; a plurality of suspension type fish reefs are arranged in the net cage, each suspension type fish reef comprises a plurality of single bodies horizontally installed on the floating rope, and each single body is a shell with a plurality of small holes formed in the shell wall. An area which is flat in terrain, relatively gentle in water flow, rich in plankton and about 10 m in water depth is selected near a sea area to be released as a domestication field, the device is put into the domestication field, and in-situ wild domestication is carried out on sepiella maindroni larvae; and the predation capability, the capability of avoiding enemies and the capability of coping with environmental stress after entering a natural sea area are improved, so that the enhancement and release effects are improved.

The invention relates to a mycorrhizal cultivation method for improving the stress resistance of pinus khasys seedlings, and belongs to the technical field of agricultural planting, the cultivation method comprises the following steps: (1) compounding fibrous belly fungi, bolete and russule virens into a microbial inoculum, and culturing to prepare microcapsules; (2) taking turf, vermiculite, humus soil and perlite as substrates, and adding a microcystis agent and a water-retaining agent; (3) the seeds are disinfected and sown, and seedlings are domesticated through light intensity gradient, water stress and temperature fluctuation; 4, during transplanting, abscisic acid is used for treating root systems, organic fertilizer is pre-filled, and mycorrhizal activation promoting liquid is perfused, through the synergistic effect of composite mycorrhizal fungi and stress domestication, the stress resistance, the survival rate and the afforestation adaptability of pinus khasys seedlings are remarkably improved.

The invention discloses a microbial remediation method and a microbial remediation system. The microbial remediation method comprises the following steps: collecting samples from a contaminated site, carrying out enrichment culture, screening and domestication to obtain various microbial strains, preparing a composite microbial agent according to a specific ratio, carrying out carrier immobilization treatment to form immobilized microbial particles, applying the immobilized microbial particles to soil in the modes of strip application, hole application and the like according to the contamination condition, or filling a suspended filler and putting the suspended filler into a water body. And environmental parameters are monitored in real time, and accurate regulation and control are performed to promote microorganisms to degrade pollutants. The microbial remediation system covers microbial culture and microbial agent preparation, putting and distribution, monitoring and regulation modules and the like, and automation and intelligentization of the whole process are achieved. The method is high in remediation efficiency, high in adaptability, low in cost, environmentally friendly and capable of effectively solving the problem of soil and water pollution.

The invention relates to the technical field of fry domestication, in particular to domestication bait and domestication method for mandarin fish fries. According to the specific technical scheme, the domestication bait for the mandarin fish fries comprises live crucian carp fries and silver carp fries, half-dead crucian carp fries and half-dead silver carp fries, mandarin fish powder, a mixture of the dead crucian carp fries and the dead silver carp fries and mandarin fish feed 0 #; a phagostimulant is added into the powder, and the phagostimulant is one of squid paste, betaine and hermetia illucens pulp. According to the three phagostimulants disclosed by the invention, the feeding domestication efficiency can be improved, the squid paste has the most obvious effect in the aspect of promoting the early growth performance and is excellent in related physiological metabolism indexes, and the betaine relieves the liver metabolism burden in the mandarin fish domestication process.

The invention relates to the field of plant tissue culture, and discloses a method for culturing tissue culture seedlings of small angel viridae, which comprises the following steps: S1, explant selection and primary culture; s2, carrying out subculture multiplication culture; s3, rooting culture; s4, domesticating and transplanting; in the primary culture stage, MS is used as a basic culture medium, a proper amount of 6-BA, NAA, sucrose and agar are added, and induction of cluster buds is promoted; in the subculture multiplication culture stage, an optimal multiplication culture medium is screened through an orthogonal test, so that the multiplication coefficient is improved; in the rooting stage, key factors such as inorganic salt concentration, NAA and banana puree in MS are optimized, and the number and quality of root systems are improved; in the domestication transplanting stage, the optimal proportion of river sand, coco coir and peat soil is researched, and the transplanting survival rate and the tissue culture seedling quality are effectively improved. By means of the method, efficient propagation of the small angel vitis vinifera and large-scale production of high-quality seedlings are achieved, and important technical support is provided for industrial popularization of the plant.

The invention discloses a method for establishing a medicinal plant litsea cubeba tissue culture seedling system. Aiming at the problems of difficulty in explant pollution and browning control, low proliferation and rooting efficiency, insufficient transplanting and domesticating survival rate and the like in establishment of a litsea cubeba tissue culture system in the prior art, the invention provides the establishment method of the medicinal plant litsea cubeba tissue culture seedling system, the germination rate of explants is 90% or above, and the transplanting survival rate in 30 days reaches 90.6%.

The invention discloses an auricularia plicata strain and a cultivation and domestication method thereof, and particularly relates to an auricularia plicata strain W-24 (Jiangxi No.1) and a cultivation and domestication method thereof, the auricularia plicata strain W-24 is preserved in China General Microbiological Culture Collection Center on June 11, 2024; the preservation number is CGMCC (China General Microbiological Culture Collection Center) The cultivation method comprises the following steps: firstly, activating auricularia plicata strains on a PDA (potato dextrose agar) comprehensive culture medium, inoculating the auricularia plicata strains into a solid culture medium, and culturing at 24-26 DEG C for 30-35 days to obtain solid stock seeds; the solid stock seeds are inoculated into cultivation bags, the inoculation amount is 10-15 g / bag, the cultivation bags are sealed, the cultivation bags are placed in a cultivation room to be cultivated in a dark place at the constant temperature of 24-26 DEG C, after cultivation is carried out for 80-85 days, hyphae grow all over the fungus bags, and fruiting management is carried out; the auricularia plicata strain W-24 provided by the invention has the characteristics of short cultivation period, high yield, strong stress resistance and the like, and has a wide market prospect.

The invention aims to provide a morchella esculenta breeding method, an obtained morchella esculenta strain and application, the morchella esculenta is named as Morchella sextella NYD1, the taxonomic name of the morchella esculenta is Morchella sextella NYD1, the morchella esculenta is preserved in Guangdong Microbial Culture Collection Center, the preservation number of the morchella esculenta is GDMCC No: 66082, and the preservation date of the morchella esculenta is April 21, 2025. The morchella strain is further utilized as a mother strain to carry out domestication and cultivation, the morchella with the fruiting yield up to 400 kg / mu or above, excellent agronomic characters and rich nutritional ingredients such as crude protein, total sugar, iron, zinc and calcium is finally obtained, the content of the crude protein is not lower than 23 g / 100 g, the content of the total sugar is not lower than 55 g / 100 g, the content of the iron is not lower than 240 mg / kg, the content of the zinc is not lower than 70 mg / kg, and the content of the calcium is not lower than 76 mg / kg.

The utility model discloses a mandarin fish domestication flushing wave making device which comprises a water inlet pipeline used for achieving water inlet and stretching into a culture pond, a wave making pipeline is arranged at the end, away from the water inlet end, of the water inlet pipeline, and a water inlet fixing assembly used for fixing the position of the water inlet pipeline is arranged on the water inlet pipeline. A wave making supporting assembly used for fixing the position of the wave making pipeline is arranged on the wave making pipeline; the wave making pipeline comprises a wave making pipe corresponding to the culture pond, a plurality of water spraying holes are formed in the wave making pipe side by side at intervals, connecting pieces used for connecting the wave making pipe with other pipelines are arranged at the two ends of the wave making pipe, and the wave making pipe can change the directions of the water spraying holes in the connecting pieces. The direction of the water spraying holes in the wave making pipe can be changed through the connecting piece, and the wave making size, flow speed, frequency and direction can be changed through the variable frequency pump and the flow adjusting valve together, so that the mandarin fish wave making device meets the feeding characteristic requirements of mandarin fish in different growth stages.

The invention provides a sandy soil root domestication layered structure improvement method which comprises the steps that a surface guide layer, a channel induction layer and a deep resource layer are sequentially arranged on the earth surface from top to bottom, in-situ sandy soil is buried in the surface guide layer, a loose channel is constructed in the channel induction layer, and the loose channel is communicated with the deep resource layer; the deep resource layer comprises a water locking substance, a water retention substance, an adsorption substance and a supporting substance; when it is detected that the EC value of the joint of the surface guide layer and the channel induction layer is larger than the preset multiple of the EC value of the joint of the channel induction layer and the deep resource layer, a drip irrigation system is started to conduct drip irrigation on the surface guide layer according to the preset water pressure and water flow so as to guide nutrients in the surface guide layer to be collected towards the deep resource layer; according to the method, replacement of soil dressing is not needed, deep pricking and field planting of plant roots are guided through water and fertilizer stress and nutrition induction mechanisms, the drought resistance and stress resistance of plants are improved, the quality of crop products is optimized, a stable water and fertilizer environment is created for growth of plants in sandy soil, and the economical efficiency and effectiveness of the improvement technology are improved.

The invention relates to marine fish breeding, and particularly discloses an indoor full-artificial breeding method for black epinephelus, which specifically comprises the key technical links of nutrient enrichment of parent fish, hormone induced spawning, artificial insemination and fertilized egg incubation, indoor breeding of fry, compound feed domestication and the like. By controlling water temperature, illumination, salinity and nutritional conditions, synchronous maturation and efficient ovulation of parent gonads of the epinephelus nigatosus are achieved, the fertilization rate reaches 95% or above, and the hatching rate exceeds 85%. According to the method, the whole-process artificial controllable breeding of the epinephelus nigatosus from the parent fish to the fry is realized, a new variety is provided for the epinephelus culture industry, and a feasible technical approach is also provided for the artificial breeding and improved variety breeding of the wild epinephelus nigatosus in the island reef of the South China Sea.

The invention belongs to the technical field of mandarin fish culture, and relates to a mandarin fish domestication survival rate prediction method and system based on ecological simulation. Establishing a quantitative relation between the ecological factors and the sample mandarin fish survival rate change slope based on the quantitative relation so as to form an evaluation model; a historical survival rate curve is generated, and the risk state is determined by comparing the current sample mandarin fish survival rate change slope with the slope of the historical survival rate curve in the corresponding time section; calculating a change path of the ecological factors in a future time period based on the evaluation model, generating a predicted survival rate curve, and outputting an analysis report containing a risk state judgment result; according to the method, a complex domestication process is converted into a quantifiable data model, and early-stage accurate early warning of the risk is realized through dynamic analysis of the change trend of the survival rate.