Agrocybe cylindracea strain and breeding method

Abstract

The invention belongs to the field of domestication and cultivation of edible mushrooms, and particularly relates to an agrocybe cylindracea strain and a domestication and cultivation method. The breeding method of the new agrocybe cylindracea strain Ganxi agrocybe cylindracea No.1 comprises the following steps: (1) activating and culturing an agrocybe cylindracea strain mother strain; (2) preparing a stock seed and a cultivar; (3) preparing a cultivation material bag; (4) culturing the cultivation bag; through sporocarp tissue separation, artificial domestication and systematic selection, an excellent new variety which can be applied to production is finally cultivated and can be used as an optimized variety for facility and factory cultivation of agrocybe cylindracea. Through domestication and cultivation, suitable growth conditions and cultivation characteristics of the 'Ganxi agrocybe cylindracea No.1' are defined, an efficient cultivation technology matched with the variety is established, and the 'Ganxi agrocybe cylindracea No.1' variety has great popularization and application potential.

Description

Technical Field

[0001] The invention belongs to the field of edible fungus domestication and cultivation, and specifically relates to a tea tree mushroom strain and a breeding method. Background Art

[0002] Artificially cultivated edible fungi originally came from the wild. Under natural conditions, wild edible fungi complete their life cycle through mycelium growth or spore reproduction. With the maturity of edible fungi pure strain isolation technology, edible fungi pure culture has been widely promoted and applied. People collect fruiting bodies from the wild, obtain cultures and wild strains through tissue separation or spore separation methods, and then obtain artificially cultivated strains by domestication and repeated selection under artificially simulated wild environmental conditions. With the gradual deepening of people's understanding of the nutritional mode and growth characteristics of wild edible fungi, the artificial domestication of wild edible fungi has received more and more attention, and the types of domesticated cultivation have become increasingly rich. At present, a considerable number of excellent edible fungi varieties in my country come from the artificial domestication and systematic breeding of wild strains. The most prominent advantage of artificially domesticated wild fungi strains is that the geographical conditions of different wild strains are quite different. In a natural environment with large differences, germplasm materials with large genetic differences may be formed, which provides an excellent source for the domestication, improvement and breeding of wild edible fungi strains.

[0003] Agrocybe aegerita belongs to the phylum Basidiomycetes, class Agaricales, order Agaricales, family Pseudoumbelliferae, and genus Cyclops. Because the earliest domesticated and cultivated strains came from the decayed stumps of Agrocybe aegerita, its commercial name is Agrocybe aegerita. Agrocybe aegerita is popular among consumers for its beautiful mushroom shape, tender cap, crisp stem, and fragrant and rich taste. According to statistics from the China Edible Fungi Association, my country's annual output of Agrocybe aegerita will exceed 400,000 tons in 2022, with an output value of over 5 billion yuan. It is one of the rare edible fungi that are currently widely cultivated. Wild Agrocybe aegerita usually grows under oil tea trees. Changes in the ecological environment have led to increasingly scarce resources for wild Agrocybe aegerita. The current status of my country's Agrocybe aegerita seed industry is that there is a lack of available germplasm resources, a lack of excellent varieties, and serious degeneration of fungal species, which has become a bottleneck restricting the sustainable development of the Agrocybe aegerita industry. Therefore, it is of great value to domesticate and improve the collected wild Agrocybe aegerita resources and create excellent cultivars.

[0004] Edible fungi distributed in nature are extremely abundant, but only dozens of species have been artificially cultivated. Therefore, the domestication and cultivation of wild edible fungi has always been a hot topic for edible fungi workers. Through domestication and cultivation, wild Agrocybe aegerita germplasm resources can be cultivated into artificially cultivated strains, which can not only enrich my country's Agrocybe aegerita cultivars and provide excellent strains for the industry, but also provide breeding materials for the selection and breeding of excellent varieties of Agrocybe aegerita, solving the current situation in my country's breeding field where there is a lack of available germplasm resources, a narrow genetic background, and insufficient parents with excellent traits. Therefore, the discovery and domestication of new strains of Agrocybe aegerita has great research and application value. Summary of the invention

[0005] The purpose of the present invention is to provide a method for breeding a new strain of Agrocybe tumefaciens "Gan Agrocybe tumefaciens No. 1". The breeding method comprises the following steps: (1) Activation culture of mother strain of Agrocybe aegerita: The purified and preserved mother strain of wild Agrocybe aegerita is transferred to the sterilized culture dish PDA medium, and cultured in a biochemical incubator in the dark for about 10 days until the plate is full of growth, thus culturing into activated mother strain; (2) Producing stock and cultivars: multiplying the activated mother stock into stock and cultivars; (3) Preparation of cultivation material bags: After the cultivation raw materials are fully mixed according to the formula, the moisture content of the culture material is controlled at 60% to 65%, and the material is packed in polypropylene plastic bags. Each bag of dry material weighs 0.5 to 0.55 kg. After packing, the bags are sealed with plastic rings and covers, and then sterilized by high pressure. After sterilization, the bags are moved to a pre-sterilized cooling room for cooling, and then inoculated in the inoculation room according to the aseptic operation specifications. (4) Cultivation in cultivation bags: When the mycelium grows to 2 / 3 of the bag, loosen the bag mouth, oxygenate the bag, and yellow water droplets begin to appear at the bag mouth and brown spots appear on the bag surface, the bag is physiologically mature; (5) Promoting bud formation: Move the mature mushroom bags into the fruiting room, adjust the ambient temperature of the fruiting room at 20-22°C, maintain the relative humidity of the mushroom room at 85%-90%, provide 200-300 lux of scattered light, and ventilate the room to keep the air fresh. When rice-like primordia appear on the surface of the bags, straighten the bag mouth and then slightly close it. After 1-2 days, the primordia differentiate into mushroom buds. (6) Cultivating mushrooms: After the mushroom buds are formed, the temperature of the mushroom-growing environment is regulated to 22-24°C and the relative humidity of the space is 90%-95%. The ventilation frequency and time are reduced to increase the carbon dioxide concentration in the mushroom room, promote the growth of the Agrocybe agrocybe stipes, and inhibit the opening of the mushroom cap. (7) Tissue separation of wild mushroom fruiting bodies: Before the fruiting bodies of Agrocybe aegerita reach 6-7 parts of maturity and the mushroom veil is broken, observe and statistically analyze the fruiting body yield and agronomic traits, select the fruiting bodies, and separate their tissues and purify them through culture. After 5 rounds of domestication and repeated selection, we finally bred excellent Agrocybe aegerita strains.

[0006] Furthermore, morphological anatomy and molecular phylogenetic analysis techniques were used to identify wild mushrooms. Morphological characteristics of wild mushroom fruiting bodies: fruiting bodies are solitary, twin or clustered, with a cap diameter of 3-10cm, initially hemispherical, dark brown to tea brown, then gradually flattened, gradually light brown to light khaki, with light edges and darker middles, sticky when deeply wet, smooth or wrinkled, with a fungus curtain attached and rolled inward when young; the flesh (except the surface and the base of the stipe) is white or dirty white, slightly tough, thicker in the middle and thinner at the edges. The stipe is medium solid, about 3-12 cm long, 0.5-2 cm thick, cylindrical, nearly equal in thickness, dirty white, darker near the base and dirty brown, and fibrous on the surface. The stipe becomes hard during maturity, with a dark sticky substance attached to the stipe, and the ring remains on the stipe or attached to the edge of the cap and falls off automatically. The gills are straight or extended, dense, unequal in length, white at the beginning, dark brown to brown when mature; the ring is superior, white at the beginning, and dark brown with basidiospores on the upper surface, membranous to silky, not easy to fall off. The size of the basidiospore is 7-10×4-6µm, oval, smooth, dark brown, and the specific fruiting body morphology, anatomy and microstructure are shown in the attached Figure 1-10 The phylogenetic tree constructed by MEGE software and NJ method based on ITS sequences showed that the wild strain was Agrocybe aegerita.

[0007] Furthermore, in step (2), the formula of the original seed and the cultivated seed includes 39% by weight of sawdust, 33% by weight of cottonseed hulls, 18% by weight of wheat bran, 8% by weight of corn flour, 1.5% by weight of lime, 1% by weight of light calcium carbonate, and a water content of 58% by weight.

[0008] Furthermore, in step (3), the culture material formula includes 36% cottonseed hulls, 29% sawdust, 22% bran, 10% corn flour, 1% soybean meal, 1% lime, and 1% light calcium carbonate by weight; the culture material sterilization conditions are 0.11-0.14 MPa pressure, and the steam temperature reaches 121-126° C. and is maintained for 2-2.5 hours.

[0009] Furthermore, in step (4), during the first 15 days of cultivation in the cultivation bag, the environment of the spawning room is controlled to have a temperature of 25-26°C, a relative air humidity of 60%-65%, a light intensity of 50-100 Lx, and a CO2 concentration of 0.04-0.1%; during the 16-50 days of cultivation in the cultivation bag, the environment of the spawning room is controlled to have a temperature of 22-24°C, a relative air humidity of 65%-70%, a light intensity of 100-200 Lx, and a CO2 concentration of 0.06-0.08%.

[0010] Furthermore, in step (5), the environment of the mushroom room is controlled to be 20-22°C, 85%-90% relative humidity, 200-300Lx light, and 0.1-0.3% CO2 concentration. When rice-like primordia appear on the surface of the mushroom bag, the mouth of the bag is straightened, and the primordia differentiate into mushroom buds after 1-2 days.

[0011] Furthermore, in step (6), the mushroom growing environment is controlled to have a temperature of 22-24°C, an air relative humidity of 90%-95%, a light intensity of 100-200 Lx, and a CO2 concentration of 0.5-0.7%.

[0012] Furthermore, in step (7), the Agrocybe aegerita fruiting bodies that fruit early, have a large number of fruiting bodies with high uniformity, thick caps, strong stipes, and slow cap opening are selected from the fruiting bags, and their tissues are separated and purified.

[0013] Furthermore, in step (8), the new tea tree mushroom strain isolated and purified is subjected to five generations of domestication cultivation and repeated selection, and finally a new tea tree mushroom strain with excellent traits is selected, and the biological characteristics and agronomic traits of the domesticated tea tree mushroom strain are clarified.

[0014] Compared with the prior art, the present invention has the following beneficial effects: (1) A new strain of Agrocybe ganensis, “Gan Agrocybe 1”, was cultivated using wild Agrocybe ganensis resources as materials. The strain’s provenance comes from a wild Agrocybe ganensis fruiting body collected from the tree stalk of an oil tea tree. The fruiting body is robust, with a thick cap and a thick stalk. It has the potential for high yield, high quality and stress resistance. Through fruiting body tissue separation, artificial domestication and systematic selection, a new excellent variety that can be used in production was finally cultivated, which can be used as a preferred variety for facility-based and factory-based cultivation of Agrocybe ganensis.

[0015] (2) Through the analysis of the biological characteristics and agronomic traits of the new strain "Gan Tea Tree Agrocybe No. 1", it was found that the strain has strong stress resistance, short cultivation cycle, and excellent commercial traits. Through domestication and cultivation, the suitable growth conditions and cultivation characteristics of "Gan Tea Tree Agrocybe No. 1" were clarified, and an efficient cultivation technology matching this variety was established. It has the characteristics of a tea tree mushroom facility cultivation variety and is of great value for promotion and application.

[0016] The strain preservation date of the present invention's "Gan Tea Tree Agrocybe No. 1" is August 12, 2024, and the preservation number is CGMCC No. 41432. The classification name is tea tree agrocybe Cyclocybe cylindracea, and the preservation unit is the General Microbiological Center of the China Microbiological Culture Collection Administration, with an address of No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing, zip code 100101. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 Schematic diagram of the fruiting body and habitat of the wild mushroom of the present invention; Figure 2 Schematic diagram of the colony of the wild mushroom strain of the present invention; Figure 3 Schematic diagram of the microstructure of the cap epidermis of the wild mushroom fruiting body of the present invention; Figure 4Schematic diagram of the microstructure of the epidermis of the stipe of the wild mushroom fruiting body of the present invention; Figure 5 Schematic diagram of the microscopic structure of the basidiospore of the wild mushroom fruiting body of the present invention; Figure 6 Schematic diagram of domestication and cultivation of Agrocybe aegerita with the "Gan Agrocybe 1" of the present invention; Figure 7 Schematic diagram of the domesticated and cultivated Agrocybe aegerita fruiting body of the present invention "Gan Agrocybe 1"; Figure 8 Schematic diagram of the cap of the domesticated and cultivated Agrocybe agrocybe of the present invention "Gan Agrocybe 1"; Fig. 9 Schematic diagram of the gills of the domesticated and cultivated Agrocybe agrocybe of the present invention "Gan Agrocybe 1"; Fig.10 Schematic diagram of the domesticated and cultivated tea tree mushroom stipe of "Gan Tea Tree Agrocybe No. 1" of the present invention. DETAILED DESCRIPTION

[0018] The technical scheme of the present invention is described clearly and completely below in conjunction with the embodiments. The described embodiments are only part of the embodiments of the present invention, not all of the embodiments. The methods are conventional methods unless otherwise specified, and the raw materials can be obtained from public commercial channels unless otherwise specified. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without making creative work are within the scope of protection of the present invention.

[0019] The present invention provides a tea tree mushroom strain and a domestication and cultivation method, comprising the steps of: obtaining a wild mushroom culture by tissue separation from a wild mushroom fruiting body collected from a wild oil tea tree, and identifying the collected wild mushroom fruiting body and the separated wild mushroom culture as wild tea tree mushrooms by morphological anatomy and molecular phylogenetic analysis. The wild Agrocybe aegerita culture was propagated into strains, and the cultivation substrate was prepared with sawdust and cottonseed hulls as the main raw materials. The culture was cultivated in cooked bags. The temperature, light, air and humidity of the environment for spawning and fruiting were regulated according to the biological characteristics of Agrocybe aegerita, the fruiting bodies of Agrocybe aegerita were cultivated, the growth traits and cultivation characteristics of the wild Agrocybe aegerita strains were analyzed, and the fruiting bodies with early fruiting, many mushroom buds, thick caps, long stipes, good color and slow opening were selected from the Agrocybe aegerita fruiting bodies, which were subjected to tissue separation and purification culture, and the pure cultures with better mycelium growth were screened for propagation into strains and the next round of domestication and cultivation. After 5 rounds of domestication, cultivation and system selection as mentioned above, a domestication and cultivation method for a new strain of wild Agrocybe aegerita was formed, and the domesticated new strain of Agrocybe aegerita was named "Gancha.Shugu No. 1".

[0020] Through the present invention, a new tea tree mushroom strain "Gan tea tree mushroom No. 1" is obtained. Figure 1-10As shown in the figure, the fruiting body morphology of this strain is as follows: solitary, twin or clustered; the cap is 3-10cm in diameter, initially hemispherical, dark brown to brown, then gradually flattened, gradually light brown to light khaki, light in edge, dark in middle, sticky when wet, smooth or wrinkled, with a veil attached and rolled inward when young; the flesh (except the surface and the base of the stipe) is white or dirty white, slightly tough, thicker in the middle and thinner at the edge; the stipe is medium solid, about 3-12cm long, 0.5-2cm thick, and round Columnar, nearly equal thickness, dirty white, darker dirty brown near the base, fibrous surface, stipe becomes hard at maturity, with dark sticky substance attached to the stipe, the ring remains on the stipe or attached to the edge of the cap and falls off automatically; the gills are straight to extended, dense, unequal in length, white at first, dark brown to brown after maturity; the ring is superior, white at first, and dark brown with basidiospores on the upper surface, membranous to silky, not easy to fall off; the basidiospore is 7-10×4-6µm in size, oval, smooth, and the spore print is dark brown. Example

[0021] The domestication and cultivation method of the new tea tree mushroom strain comprises the steps of: activating and culturing the mother strain of the wild tea tree mushroom strain, adopting a solid strain production method to multiply the original strain and the cultivated strain, adopting a cooked material bag cultivation method to make a cultivation bag, carrying out bag cultivation, bud inducing and mushroom cultivation management, analyzing the growth characteristics and cultivation characteristics of the wild tea tree mushroom strain, screening out fruiting bodies with early mushroom production, many mushroom buds, long stems, good color and slow cap opening, carrying out tissue separation and purification culture to obtain tea tree mushroom strains with better characteristics, carrying out 5 rounds of domestication cultivation and system selection according to the above cultivation process, finally breeding an excellent tea tree mushroom strain, and naming the strain as "Gan Tea Tree Mushroom No. 1". The specific method and steps are as follows: (1) Activation culture of mother strain of Agrocybe aegerita: The purified and preserved mother strain of wild Agrocybe aegerita is transferred to the PDA culture medium in a sterilized culture dish, and cultured in a biochemical incubator in the dark for about 10 days until the mycelium fills the culture dish and the activated mother strain is cultivated.

[0022] (2) Preparation of stock and cultivar: Preparation of culture medium for stock and cultivar, and propagation of activated mother stock into stock and cultivar. The formula of stock and cultivar includes 39% by weight of sawdust, 33% by weight of cottonseed hull, 18% by weight of wheat bran, 8% by weight of corn flour, 1.5% by weight of lime, 1% by weight of light calcium carbonate, and 58% by weight of water.

[0023] (3) Preparation of cultivation material bags: The cultivation material is prepared according to the cultivation material formula, which includes 36% cottonseed hulls, 29% sawdust, 22% bran, 10% corn flour, 1% soybean meal, 1% lime, and 1% light calcium carbonate by weight. After the cultivation materials are fully mixed, the moisture content of the culture material is controlled at 60% to 65%, and the bags are packed in polypropylene plastic bags, with each bag containing 0.5 to 0.55 kg of dry material. The bags are sealed with plastic rings and covers and then sterilized by high pressure. The sterilization conditions of the cultivation material are 0.11 to 0.14 MPa pressure, and the steam temperature reaches 121 to 126°C and is maintained for 2 to 2.5 hours. After the material bags are sterilized, they are moved to a pre-sterilized cooling room for cooling, and inoculated in the inoculation room according to the aseptic operation specifications.

[0024] (4) Cultivation in cultivation bags: After inoculation, move the bags into the cultivation room. For the first 15 days of cultivation in the cultivation bags, adjust the environment in the room to 25-26℃, 60%-65% relative humidity, 50-100Lx light, and 0.04-0.1% CO2 concentration. For 16-50 days of cultivation in the bags, adjust the environment in the room to 22-24℃, 65%-70% relative humidity, 100-200Lx light, and 0.06-0.08% CO2 concentration. When the mycelium grows to 2 / 3 of the bag, loosen the bag mouth and oxygenate the bag. When yellow water droplets begin to appear at the bag mouth and brown spots appear on the surface, it indicates that the bag is physiologically mature and the mature bag is moved into the fruiting room.

[0025] (5) Management of budding: After the mushroom bags are moved into the fruiting room, the environmental conditions in the fruiting room are adjusted to 20-22℃, 85%-90% relative humidity, 200-300Lx light, and 0.1-0.3% CO2 concentration. When rice-like primordia appear on the surface of the bags, straighten the bag mouth, and the primordia will differentiate into mushroom buds after 1-2 days.

[0026] (6) Mushroom cultivation management: After the mushroom buds are formed, the environmental conditions for mushroom production should be adjusted to a temperature of 22-24°C, a relative humidity of 90%-95%, a light intensity of 100-200 Lx, and a CO2 concentration of 0.5-0.7%.

[0027] (7) Fruiting body tissue separation: Before the fruiting bodies reach 6-7 parts of maturity and the fungus curtain has not broken, observe and statistically analyze the fruiting body yield and agronomic traits, select fruiting bodies that produce mushrooms early, have a large number of uniform fruiting bodies, thick stipes, and thick caps from the fruiting bags, and perform tissue separation and purification culture on them. After five generations of domestication and cultivation experiments and repeated selection, a new strain of Agrocybe aegerita with excellent traits was finally bred. This strain has strong stress resistance, a short cultivation cycle, excellent commercial traits, and has the characteristics of a tea tree mushroom cultivar that can be cultivated in facilities. This strain was named "Gan Tea Tree Agrocybe No. 1".

Claims

1. A new strain of Agrocybe ganensis, Agrocybe ganensis 1, whose deposit number is CGMMCC NO41432.

2. A method for breeding a new tea tree mushroom strain Gan tea tree mushroom No. 1, the breeding method comprising the steps of: (1) Activation culture of mother strain of Agrocybe aegerita: The purified and preserved mother strain of wild Agrocybe aegerita is transferred to the sterilized culture dish PDA medium, and cultured in a biochemical incubator in the dark for about 10 days until the plate is full of growth, thus culturing into activated mother strain; (2) Producing stock and cultivars: multiplying the activated mother stock into stock and cultivars; (3) Preparation of cultivation material bags: After the cultivation raw materials are fully mixed according to the formula, the moisture content of the culture material is controlled at 60% to 65%, and the material is packed in polypropylene plastic bags. Each bag of dry material weighs 0.5 to 0.55 kg. After packing, the bags are sealed with plastic rings and covers, and then sterilized by high pressure. After sterilization, the bags are moved to a pre-sterilized cooling room for cooling, and then inoculated in the inoculation room according to the aseptic operation specifications. (4) Cultivation in cultivation bags: When the mycelium grows to 2 / 3 of the bag, loosen the bag mouth, oxygenate the bag, and yellow water droplets begin to appear at the bag mouth and brown spots appear on the bag surface, the bag is physiologically mature; (5) Promoting bud formation: Move the mature mushroom bags into the fruiting room, adjust the ambient temperature of the fruiting room at 20-22°C, maintain the relative humidity of the mushroom room at 85%-90%, provide 200-300 lux of scattered light, and ventilate the room to keep the air fresh. When rice-like primordia appear on the surface of the bags, straighten the bag mouth and then slightly close it. After 1-2 days, the primordia differentiate into mushroom buds. (6) Cultivating mushrooms: After the mushroom buds are formed, the temperature of the mushroom-growing environment is regulated to 22-24°C and the relative humidity of the space is 90%-95%. The ventilation frequency and time are reduced to increase the carbon dioxide concentration in the mushroom room, promote the growth of the Agrocybe agrocybe stipes, and inhibit the opening of the mushroom cap. (7) Tissue separation of wild mushroom fruiting bodies: Before the fruiting bodies of Agrocybe aegerita reach 6-7 parts of maturity and the mushroom veil is broken, observe and statistically analyze the fruiting body yield and agronomic traits, select the fruiting bodies, and separate their tissues and purify them through culture. After 5 rounds of domestication and repeated selection, we finally bred excellent Agrocybe aegerita strains.

3. The method for breeding the new tea tree mushroom strain Gan tea tree mushroom No. 1 according to claim 2, characterized in that: The wild mushrooms were identified by morphological anatomy and molecular phylogenetic analysis techniques. The morphological anatomy and microstructural characteristics of the fruiting bodies are as follows: the fruiting bodies are solitary, twin or clustered, with a cap diameter of 3-10 cm, initially hemispherical, dark brown to tea brown, then gradually flattened, gradually light brown to light khaki, with light edges and darker middles, sticky when deeply wet, smooth or wrinkled, with a veil attached and rolled inward when young; the flesh is white or dirty white, slightly tough, thicker in the middle and thinner at the edges; the stipe is medium solid, about 3-12 cm long, 0.5-2 cm thick, cylindrical, nearly equal in thickness, dirty white, darker near the base and dirty brown, with a fibrous surface; the stipe becomes hard in maturity, with a dark sticky substance attached to the stipe, and the ring remains on the stipe or attached to the edge of the cap and falls off automatically; the gills are straight to extended, dense, unequal in length, white at the beginning, and tea brown to brown after maturity; The ring is located on the upper part, initially white, and then the upper surface becomes dark brown with basidiospores, which are membranous to silky and not easy to fall off; the basidiospores are 7-10×4-6µm in size, oval, smooth, and dark brown.

4. The method for breeding the new tea tree mushroom strain Gan tea tree mushroom No. 1 according to claim 2, characterized in that: In step (2), the formula of the original seed and the cultivated seed includes 39% by weight of sawdust, 33% by weight of cottonseed hulls, 18% by weight of wheat bran, 8% by weight of corn flour, 1.5% by weight of lime, 1% by weight of light calcium carbonate, and 58% by weight of water content.

5. The method for breeding the new tea tree mushroom strain Gan tea tree mushroom No. 1 according to claim 2, characterized in that: In step (3), the culture material formula includes 36% by weight of cottonseed hulls, 29% by weight of sawdust, 22% by weight of bran, 10% by weight of corn flour, 1% by weight of soybean meal, 1% by weight of lime, and 1% by weight of light calcium carbonate; the sterilization conditions are as follows: at a pressure of 0.11 to 0.14 MPa, the steam temperature reaches 121 to 126° C. and is maintained for 2 to 2.5 hours.

6. The method for breeding the new tea tree mushroom strain Gan tea tree mushroom No. 1 according to claim 2, characterized in that: In step (4), during the first 15 days of cultivation in the cultivation bag, the temperature of the spawning room is 25-26°C, the relative humidity of the air is 60%-65%, the light intensity is 50-100 Lx, and the CO2 concentration is 0.04-0.1%; during the 16-50 days, the temperature of the spawning room is 22-24°C, the relative humidity of the air is 65%-70%, the light intensity is 100-200 Lx, and the CO2 concentration is 0.06-0.08%.

7. The method for breeding the new tea tree mushroom strain Gan tea tree mushroom No. 1 according to claim 2, characterized in that: In step (5), the environment of the fruiting room is controlled to be 20-22°C, 85%-90% relative humidity, 200-300Lx light, and 0.1-0.3% CO2 concentration. When rice-like primordia appear on the surface of the bag, the bag mouth is straightened, and the primordia differentiate into mushroom buds after 1-2 days.

8. The method for breeding the new tea tree mushroom strain Gan tea tree mushroom No. 1 according to claim 2, characterized in that: In step (6), the temperature of the mushroom growing environment is controlled to 22-24°C, the relative humidity is 90%-95%, the light intensity is 100-200Lx, and the CO2 concentration is 0.5-0.7%.

9. The method for breeding the new tea tree mushroom strain Gan tea tree mushroom No. 1 according to claim 2, characterized in that: In step (7), the fruiting bodies with early fruiting, many mushroom buds, long stipes, good color and slow opening are selected from the Agrocybe aegerita fruiting bodies, and the tissues are separated and purified and cultured.

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