382 results about "Industrial fermentation" patented technology

The invention relates to a microorganism cleaning bactericide capable of removing multiple poisonous and harmful substances and a preparation method thereof. The microorganism cleaning bactericide comprises the mixture of compound balsamic extract liquor and the fermentation product of compound microbial bacterium system protospecies liquid in an industrial fermentation culture solution. The invention utilizes the high-efficiency adsorption, assimilation and metabolism of the microbial bacterium system to purify and treat aldehydes, benzenes, alcohols, esters, phenols, amines, alkanes, sulfur-containing substances, TVOC, putrefying bacteria secreta, obnoxious gases of pathogenic bacteria, pathogenic bacteria secreta and the like, or the harmful substances of the solution thereof by the degradation and decomposition processes; harmful substances, such as formaldehyde, benzene, volatile organic compounds and the like in environment, which are released by housing decoration, office furniture and new car decoration, are converted into nontoxic, harmless and scentless substances; and meanwhile, the invention also performs the functions of sterilization to the pathogenic bacteria and the secreta thereof and realizes the targets of thoroughly improving air quality and protecting the health of people.

The invention discloses a technique for rapidly extracting ramie hemp erwinia bacterium factory production fermentation, the technique comprises preprocessing material, immersing or sprinkling and inoculating, immersing or fermenting, deactivating with hot water, and the procedure of circulation washing, water dressing and fiber finish, erwinia bacterium which has broad-spectrum and high effective to degumming, pulping and saccharifying of herb fiber material, and bacterial agent preparation and activated technical parameter. The erwinia bacterium addicts the mannose, the peak for secreting non-cellulose to degrade key enzyme (pectic enzyme, beta-mannase and xylanase) can be achieved through purely culturing for 7h to 9h. Bacterial agent is inoculated on herb fiber material such as ramie and the like for activating the bacterial agent for 5h-6h, fermentation forage fodder turns to blue after fermenting for 5h-6h, pectine, hemicellulose and partial lignin which are partially degraded and associated in fiber cell are stripped, non-cellulose residues which are absorbed on fiber can be removed by means of the flushing of high-pressure flushing, and the purpose of extracting net fiber can be reached.

The invention provides a method for separating and purifying N-acetylneuraminic acid produced by microbiological fermentation. The method is characterized by comprising the following steps: by taking fermentation liquid in fermenting production of N-acetylneuraminic acid by escherichia coli as a raw material, sterilizing; removing proteins; decoloring; desalting; crystallizing and the like to obtain a high purity N-acetylneuraminic acid product. Through tests, the product purity is at least 98%, and the requirements in the fields of foods, healthcare, medicines, cosmetics and the like can be satisfied. Moreover, the method is simple and easy to operate and is particularly suitable for industrial fermentation production of N-acetylneuraminic acid.

The present invention relates to biological bacillus lipopeptide pesticide and its application, and belongs to the field of biotechnology. The lipopeptide pesticide is prepared through fermentation culture in culture medium of glucose, cane sugar or corn slurry at temperature of 30-37 deg.c, rotation speed of 300-1000 rpm and dissolved oxygen of 30% for 30-36 hr; centrifugation to collect supernatant, adding HCl to regulate pH to 2.0-2.5, centrifugal extraction to collect supernatant, methanol or ethanol extraction, merging extracted liquid, and vacuum drying. The present invention is used in the green prevention and control of pests and diseases of field.

The invention is suitable for the technical field of recycling organic wastes, and discloses a flora for recycling treatment of organic wastes. The flora for recycling treatment of organic wastes is efficiently compounded from a variety of bacteria such as bacillus subtilis, aspergillus niger, saccharomycete and soil flora, wherein the soil flora is extracted from the natural world and comprises three or more of bacillus megatherium, bacillus mucilaginosus, bacillus licheniformis, bacillus thuringiensis, bacillus laterosporus, bacillus mucilaginosus, streptomyces jingyangensis, mycorrhiza fungi, azotobacter vinelandii, photosynthetic bacteria, bacillus coagulans, aspergillus oryzae, and paecilomyces lilacinus. According to the flora for recycling treatment of organic wastes, disclosed by the invention, by virtue of mutual promotion, mutual coordination and combined action of a variety of beneficial microorganisms, the organic wastes are recycled, environmental pollution is reduced, and wastes are changed into valuables; and the flora for recycling treatment of organic wastes can be used for producing industrial fermentation products and improving the health and growth conditions of animals, and can also improve the current situation of serious soil hardening at present and improve soil quality, thereby promoting the rapid development of agricultural economics.

The invention relates to the genetic engineering field, especially to a strain Bispora sp.MEY-1 which produces an acidophilic beta-mannanase and the acidophilic beta-mannanase MAN5A got from the strain having an amino acid sequence shown as SEQ ID NO.1 or 2, a gene man5A for coding said beta-mannanase having a nucleotide sequence shown as SEQ ID NO.1 or 2, and a recombinant vector containing said gene and applications. The beta-mannanase of the invention has the following advantages: the optimum pH 1.0-1.5, the optimum temperature 65 DEG C, good pH stability and thermostability, high specific activity, good protease resistance and easy for industrial fermentation production. The product of the invention can be widely used for the animal feeding-stuffs, the food, the medicament, the brewing and the energy industry as a novel enzyme preparation.

A method for producing the high-value ketocarotenoid astaxanthin by the green microalga Chlorella zofingiensis in dark-heterotrophic cultures shows excellent growth and high-yield astaxanthin production on glucose-supplemented media in the dark. The specific growth rate and astaxanthin yield can be as high as 0.031 h⁻¹, and 10.3 mg l⁻¹, respectively, which are the highest so far reported in heterotrophic algal cultures. The light-independent astaxanthin-producing ability of Chlorella zofingiensis can be employed for commercial production of astaxanthin using industrial fermenters.

The invention discloses novel halomonas sp. HS-2255 which can be used for producing tetrahydropyrimidine, and a mutant strain thereof. The preservation number of the novel halomonas sp. HS-2255 is CGMCC (China General Microbiological Culture Collection Center) No. 6248. The invention further discloses a culture method of the strain and a method for producing the tetrahydropyrimidine by fermentation. The strain has a higher tetrahydropyrimidine yield in a culture medium with a carbon source and a nitrogen source which are assimilable, and lower NaCl content, and the content of a byproduct hydroxyl tetrahydropyrimidine is lower, and therefore, the novel halomonas sp. HS-2255 can be used for industrial fermentation production of tetrahydropyrimidine, and has good application prospect.

The invention discloses a fluid supplementary food weighing and metering control device, which comprises a supplementary food cup, a valve system, a fermentation tank and a supplementary food tank, and is characterized by also comprising a weighing system and an electronic monitoring system, wherein the weighing system comprises a weighting sensor, a suspension arm, a fixed bracket and a weighing cup, the supplementary food cup is connected with the weighting sensor to weigh the supplementary food in the supplementary food cup, the electronic monitoring system comprises an A/D conversion module, an alarming and display module, a keyboard, a mouse, a touch screen, a comparator, a setter, a CPU and a food supplementation speed controller. As the supplementary food is metered by the weighing device, the device has the advantages of up to 0.5 percent high food supplementation accuracy and providing a reliable guarantee for the optimization and amplification of an industrial fermentation process.

This invention discloses a preparation method of a wild pawpaw fruit wine. The method comprises the following steps of: after treating rice pulp with a liquefying enzyme and a saccharifying enzyme, mixing the treated rice pulp and wild pawpaw juice according to a proportion of 1:1-1.5(W/W) and fermenting the mixture to obtain the wild pawpaw fruit wine. Compared with the prior art, the preparation method of the invention simplifies the production process, shortens the production period, reduces the production cost, improves the economic benefit and contributes to the industrial fermentation mass production of the wild pawpaw fruit wine. The wild pawpaw fruit wine prepared by the method is mellow, has the faint scent of wild pawpaw, is amber and transparent in colour, offers a pure mouthfeel and long lingering aftertaste, better retains the nutritional value and special flavor of the wild pawpaw and also has better nutritional and healthy functions of fruit wines.

The invention relates to a method for preparing a water soluble quick-acting organic fertilizer from activated sludge. In the method, the activated sludge is used as a main material, livestock and poultry excrement and sorted organic domestic garbage or furfural slag, xylitol slag, industrial fermentation waste residue or crop straws are used as auxiliary materials, and the main material and the auxiliary materials are subjected to chemical catalytic degradation, ammonia-fixing treatment, solid-liquid separation, membrane concentration to remove heavy metal, vacuum evaporation and concentration, and dehydration to form a closed cycle without three wastes; the prepared water soluble quick-acting organic fertilizer with high additional value can be used as a base fertilizer and an additional fertilizer. The method has the advantages of greatly reducing the using amount of the fertilizer, greatly improving nutrient utilization rate, greatly reducing pollution due to wastage of the fertilizer, improving the quality of agricultural products, optimizing soil structure, and quickly increasing soil fertility.

The invention provides engineering bacteria based on beta-glucosidase in the technical field of bioengineering and an implementation method thereof. A beta-glucosidase gene is cloned from Streptomyces griseorubens (Streptomyces griseorubens) JSD-1, and then is connected to an expression vector to obtain a recombinant expression vector, and the recombinant expression vector is further introduced into escherichia coli and is induced to synthesize beta-glucosidase. The engineering bacteria provided by the invention overcomes the defects that the beta-glucosidase in the prior art is generally endoenzyme and the expression quantity is very low, and the like, the beta-glucosidase gene of the invention is expressed by genetically engineered bacteria to prepare beta-glucosidase, and a new method is provided for producing beta-glucosidase by industrial fermentation.

The invention discloses an antrodia cinnamomea growth medium and a preparation method and application thereof, belonging to the field of bioengineering technology. The antrodia cinnamomea growth medium consists of the following raw materials: 100-250ml of 2% boiled liquid of cinnamomum camphora leaves, 150-250g of potato, 12-18g of glucose, 1-5g of sodium chloride, 0.005-0.03g of cinnamomum camphora oil and water with the rest volume of 1,000ml; the pH is 6.5-7.0. Compared with the prior art, the antrodia cinnamomea growth medium disclosed by the invention has the beneficial effects that (1) the medium is simple to prepare and convenient to store, the adopted technology is mature, and the medium contains all nutritional ingredients required for the antrodia cinnamomea growth and is suitable for the culture and large-scale industrial fermentation production of antrodia cinnamomea; (2) the cultured antrodia cinnamomea mycelia have high biomass and high intracellular triterpene output, wherein the biomass of mycelia reaches 12.1-14.2g/1,000ml, and the intracellular triterpene output reaches 33.32-46.54mg/g.

The invention discloses an industrial fermentation method of riboflavin, which comprises the following steps of: filling a seed culture medium into a 10m<3> seed tank, and performing oxic cultivation; and filling a fermentation medium into a 200m<3> fermentation tank for fermentation, mechanically stirring, adding sterilization starch glucose liquid, stopping fermenting till that the glucose is completely consumed or the dissolved oxygen is raised to be 60%, and extracting the riboflavin from the fermentation liquid. After the industrial fermentation method is used, the conventional riboflavin material-supplementing fermentation technology is changed, the low-concentration raw material cultivating bacteria is adopted, the material is quantificationally supplemented according to the changeof the dissolved oxygen of the fermentation solution, the excessive multiplication of the thallus can be effectively controlled, the deviation of the optimum control temperature caused by the excessive supersession can be avoided, the defects of the insufficient ventilation and oxygen supply can be remitted, and the thallus vigor can be kept, so that the indusial fermentation unit of the riboflavin is improved by more than 18g/L, and the yield is increased by 20% compared with the common fermentation.

The invention discloses a method for producing grease containing triglyceride type DHA by Crypthecodinium cohnii industrial fermentation. The method comprises the following steps: taking the Crypthecodinium cohnii as a strain, employing a culture medium comprising a carbon source, a nitrogen source and inorganic salts, and obtaining the DHA grease by fermentation; the nitrogen source contains hydrolytic liquor of slow-release bean pulp powder as nitrogen source. The grease produced by the fermentation contains more than 50% of DHA, among which EPA content is below 1%, and the DHA productivity reaches 4.6g/L per day. The DHA has high output, high purity, safe and reliable quality, low production cost, stable production, and little or no EPA; The method can produce environment-friendly and safe high DHA content triglyceride type DHA grease in quantity with low cost.

The present invention relates to one kind of genetic engineering bacteria, one kind of soybean disease causing variety of clove pseudomonads with preservation number of CGMCC 1276, and its construction process and use. The genetic engineering bacteria is obtained through inserting Tn5 transposon S17-1 into the genome of the initial strain to deactivate its temperature controlling gene. The strain may be used in industrial fermentation production of crown bacterin at 26-28 deg.c, in the yield near the initial bacteria and up to 35-40 mg/L.

The invention relates to a purification compound bacterium. The compound bacterium consists of 10-30% of beer yeast, 10-30% of plant lactic bacillus, 10-30% of lactobacillus acidophilus, 10-30% of bacillus subtilis, 5-15% of bacillus cereus and 5-15% of rhizobium radiobacter. Furthermore, the invention relates to a purification compound probiotic preparation and a preparation method thereof. The preparation method comprises the steps of inoculating the purification compound bacterium on a comprehensive liquid culture medium, and implementing multi-strain compound fermentation within a temperature range of 28-35 DEG C. By combining various aerobic, facultative and anaerobic strains, the purification compound probiotic preparation is prepared through an advanced industrial fermentation technology. The compound probiotic preparation has such advantages as being wide in application scope, high in treatment efficiency, free from secondary pollution, simple in equipment required, convenient for operation, low in operation cost, low in environmental load, and simple and convenient maintenance and management.

The invention relates to a method for preparing mixed fodder of stalk and trester through biofermentation, which comprises mixing stalk 40-50 parts, trester 30-50 parts, bran 10-20 parts, ammonia sulfate 2-4 parts, ultramide 2-4 parts and EM dilution homogeneously, charging into an industrial fermentation tank, evacuating air to obtain anaerobic state, controlling the temperature within the range of 28-30 deg C. , fermenting five days.

The invention relates to a Luteimonas sp. and its application. The Luteimonas sp. HB-2CGMCC No.6457 has been preserved in China General Microbiological Culture Collection Center since August 17, 2012. The Luteimonas sp. is used for oil reservoir flooding. The above strain is fermented in a 12m<3> industrial fermentation tank at 45DEG C for 48h, a bacterial solution with the mass concentration of 1% obtained after the fermentation and a nutritional liquid with the mass concentration of 1% are injected into the oil reservoir from a water injection well, and the injection amount is 0.07PV, so the viscosity of crude oil decreases by 53%, the recovery efficiency increases by above 5.0%, and the input-output ratio is 1:6.4.

The invention relates to the genetic engineering field, especially to a strain Bispora sp.MEY-1 which produces an acidic xylanase and the acidic xylanase XYL10A got from the strain having an amino acid sequence shown as SEQ ID NO.1 or 2, a gene for coding said xylanase having a nucleotide sequence shown as SEQ ID NO.1 or 2, and application of a recombinant vector, a recombinant strain and a recombinant enzyme containing said gene. The xylanase of the invention has the following advantages: the optimum pH 2.4, the optimum temperature 80 DEG C, good pH stability and thermostability, specific activity 5437 U/mg, good protease resistance and easy for industrial fermentation production. The product of the invention can be widely used for the animal feeding-stuffs, the food, the medicament, the brewing and the energy industry as a novel enzyme preparation.

The invention provides a method for producing poly-N-acetylneuraminic acid by fermentation of escherichia coli CGMCC NO.5585 and a purification method of the poly-N-acetylneuraminic acid. The high-purity poly-N-acetylneuraminic acid without endotoxins is further obtained and can meet the production requirements of foods, cosmetics and medicaments. According to the methods provided by the invention, the fermentation raw material uses cheap glucose to replace more expensive sorbitol, the yield of 5-6g/L can be still obtained during the fermentation stage, and the methods are particularly suitable for industrial fermentation production of PSA (polysialic acid); and finally, through the follow-up purification step, the high-purity PSA with the polymerization degree of at least above 70000Da, the purity of at least more than 95% (HPLC(high performance liquid chromatography)) and the yield of 4-4.5g/L can be obtained.