60 results about "Industrial fermentation" patented technology

This invention discloses a *Propionibacterium japonicum* strain selected through space-based combined chemical mutagenesis and its mutagenesis, screening, and propionic acid fermentation methods. Using *Propionibacterium japonicum* strain CICC22741 from the China Industrial Microbiological Culture Collection Center as the starting strain, this invention employs space-based mutagenesis via the "Shijian-19" satellite combined with ethyl methanesulfonate chemical mutagenesis. After two rounds of mutagenesis screening, a high-propionic acid-producing strain, *Propionibacterium japonicum* DJ04-16, was obtained. This strain was deposited at the China General Microbiological Culture Collection Center on June 16, 2025, with accession number CGMCC No. 34914. Subsequently, the propionic acid fermentation conditions of *Propionibacterium japonicum* DJ04-16 were optimized. Validation was performed in a 1-ton fermentation tank. After 96 hours of fermentation, the highest propionic acid yield reached 67.36 g / L, with a fermentation intensity of 0.702 g / L / h. This strain exhibits good industrial fermentation potential.

The invention discloses an Escherichia coli recombinant bacterium capable of efficiently expressing dsRNA, the Escherichia coli recombinant bacterium is an rnc gene defect type TG1 strain, and the following plasmids are transferred: a first plasmid comprising a T7 RNA polymerase expression module, a second plasmid comprising a dsRNA polymerase expression module, a third plasmid comprising a dsRNA polymerase expression module, and a fourth plasmid comprising a dsRNA polymerase expression module; and a second plasmid comprising a dsRNA expression module. The invention also discloses a method for producing the dsRNA. The invention also discloses an application of the recombinant escherichia coli in preparation of nucleic acid pesticides. The invention provides a novel rnc gene defect type escherichia coli recombinant bacterium for dsRNA production in the field of nucleic acid pesticides. The rnc knockout of the strain has no obvious influence on the growth rate of the strain, and the strain has great industrial fermentation potential. According to the recombinant bacterium, a double-plasmid expression system is adopted, and meanwhile, the dsRNA expression module structure of double T7 terminators is utilized to optimize and improve the expression quantity and purity of dsRNA synthesis.

The invention provides a fermentation device based on multi-physical field coordination and application thereof. The fermentation device comprises a fermentation tank unit, an annular magnetic field generation unit, a pulsed electric field generation unit and a control unit. The stable and energy-free background magnetic field is provided through the permanent magnet array, the background magnetic field and the pulsed electric field form synergy, the metabolism efficiency of microorganisms is remarkably improved, the operation cost is low, the device structure is simple, the device can be flexibly deployed, and the device is suitable for large-scale deployment and transformation on an industrial fermentation tank needing long-term and stable operation; a static background magnetic field generated by the permanent magnet is highly stable, is not influenced by fluctuation of a power grid, and is mutually independent and non-interfering with the distribution of a pulse electric field, so that the reliability and repeatability of the process are ensured.

The invention belongs to the technical field of microorganisms, a streptococcus zooepidemicus mutant strain CGMCC (China General Microbiological Culture Collection Center) No.26991 is screened out, the streptococcus zooepidemicus mutant strain is obtained through screening by adopting a phage secondary infection method, resistance experiments and genetic stability experiments prove that the screened strain is an anti-phage strain, the resistance can be stably inherited, and the strain can be used as a high-yield streptococcus zooepidemicus mutant strain. The strain not only has good resistance to bacteriophages, but also has excellent high-yield hyaluronic acid characteristics, and can be used as an industrial fermentation strain of hyaluronic acid.

The application discloses a kind of genetic engineering escherichia coli plasmid expression culture medium and fermentation process, including basic medium and feed medium;The basic medium includes glycerol, yeast hydrolysate, yeast peptone, L-glutamic acid, L-valine, L-isoleucine, arginine, tyrosine, lysine, proline, glycine, ammonium sulfate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, citric acid, magnesium sulfate, ferrous sulfate, zinc sulfate, calcium chloride, manganese chloride, cobalt chloride, copper chloride, sodium ethylenediaminetetraacetate;Feed medium includes glycerol, citric acid, magnesium sulfate, ferrous sulfate, zinc sulfate, calcium chloride, manganese chloride, cobalt chloride, copper chloride, sodium ethylenediaminetetraacetate.The application inhibits the accumulation of harmful metabolites, and creates the optimal intracellular environment for stable replication of plasmid.In industrial fermentation scale, the amount of plasmid harvested per liter of fermentation broth is more than 1 gram, and the supercoiling ratio of plasmid DNA is stably maintained at more than 90%, with minimal batch-to-batch variation.

The invention provides a method and a kit for detecting the content of grease in yeast. The method for detecting the content of grease in yeast comprises the following steps: S1, preparing a sodium chloride solution with a series of concentration gradients; s2, sequentially adding bacterial liquid containing yeast to be detected into the sodium chloride solution, uniformly mixing, then carrying out centrifugal treatment, and after the centrifugal treatment, observing the initial suspended salinity, the middle suspended salinity and the complete suspended salinity of the yeast in the sodium chloride solution; s3, determining the grease content in the to-be-detected yeast according to the initial suspension salinity, the middle suspension salinity and the complete suspension salinity. According to the method for detecting the content of the grease in the yeast cells by using the saline water with different concentrations, the range of the content of the grease in the yeast cells, which can be detected, is about 10%-60%, the detection precision is about 10%, and the method has important significance on rapid detection of the content of the grease in industrial fermentation of the yeast.

This invention belongs to the field of microbial metabolic engineering and synthetic biology, specifically relating to a strain for high-yield... R,R -2,3-Butanediol ( R,R Salt-tolerant Jeju Bacillus DT01 IM3 DLB6 (-2,3-BDO), production strain constructed from this strain and its application in fermentation production. R,R Applications of -2,3-BDO. This invention screens a high-yielding strain through heavy ion beam irradiation mutagenesis. R,R Jeju Bacillus DT01 IM3 DL B6 with -2,3-BDO, preservation number CGMCC No. 37311. This strain exhibits excellent salt tolerance and low endotoxin properties. The production strain constructed from it has extremely high product synthesis capabilities, enabling high-concentration, high-intensity industrial fermentation production.

The invention provides a sensor-based cordyceps militaris fermented beverage fermentation evaluation method and system, and relates to the technical field of fermentation engineering.Multi-frequency ultrasonic signals are transmitted and received through an ultrasonic transceiver array deployed on the outer wall of a fermentation tank; and reconstructing the signal into an acoustic parameter three-dimensional distribution image of the fermentation liquid in the fermentation tank by utilizing a tomography algorithm. And based on a pre-established mapping relation, mapping the acoustic parameter three-dimensional distribution image into three-dimensional distribution of the fermentation state parameters. And finally, based on the three-dimensional distribution of the fermentation state parameters, evaluating the overall real state of the internal environment of the fermentation tank to obtain spatial non-uniformity quantitative data of the internal environment of the fermentation tank, and generating an instruction for regulating and controlling the fermentation process according to the data. The method effectively solves the problem that monitoring of a traditional local sensor is not accurate due to non-uniformity of the internal environment of a large industrial fermentation tank in the prior art.

The application discloses a recombinant Escherichia coli for efficiently expressing dsRNA, which is a TG1 strain with gene defects and into which the following plasmids are introduced: a first plasmid comprising a T7 RNA polymerase expression module; and a second plasmid comprising a dsRNA expression module. rnc The application also discloses a method for producing dsRNA, and application of the recombinant Escherichia coli in preparing nucleic acid pesticides. rnc The application provides a new recombinant Escherichia coli with gene defects for producing dsRNA in the field of nucleic acid pesticides. rnc The knockout has no significant influence on the growth rate of the recombinant Escherichia coli, and the recombinant Escherichia coli has great industrial fermentation potential. The recombinant Escherichia coli adopts a double-plasmid expression system, and the dsRNA expression module structure with double T7 terminators is used to improve the expression amount and purity of dsRNA synthesis.

The invention provides a method for producing propionic acid and calcium salt thereof through fermentation, and belongs to the technical field of agricultural microbial fermentation. The method for producing propionic acid by fermentation comprises the following steps: (1) activating acid-producing propionibacterium with the preservation number of CGMCC1.2230, inoculating the acid-producing propionibacterium to a special seed culture medium for propionic acid, and standing and culturing for 48-72 hours under the anaerobic condition at the temperature of 37 + / -0.5 DEG C; and (2) inoculating the obtained bacterial liquid into a fermentation medium according to an inoculation ratio of 2.8-3.2%, fermenting under an anaerobic condition at 37 + / -0.5 DEG C and a stirring speed of 80-100rpm until the content of propionic acid is not obviously changed, and adjusting the pH value by using a calcium hydroxide suspension with a solid-to-liquid ratio of 15-20% during fermentation. According to the method, the yield of propionic acid produced by fermentation of the propionibacterium propionate is increased by adjusting the proportion of fermentation substrates and controlling the fermentation pH in a segmented mode, the yield of propionic acid produced by fermentation of the propionibacterium propionate can reach 16.23 g / L, and the production intensity is 0.225 gL <-1 > h <-1 >. The mixed fermentation ensures the realization of high-titer propionic acid production, and provides a theoretical basis for increasing the yield of propionic acid produced by industrial fermentation to a certain extent.

The application discloses a Pediococcus pentosaceus with the functions of lowering cholesterol and inhibiting bacteria and a high-density industrial production fermentation medium of the Pediococcus pentosaceus, the Pediococcus pentosaceus is Pediococcus pentosaceus PG-2, and the preservation number is GDMCC No: 62392.The high-density industrial fermentation medium of the Pediococcus pentosaceus PG-2 contains 40 g / L of sugarcane molasses liquid, 20 g / L of corn syrup dry powder, 1.5 g / L of dipotassium hydrogen phosphate, 5 g / L of sodium acetate, 1 mL / L of Tween 80, 2 g / L of triammonium citrate, 0.2 g / L of magnesium sulfate heptahydrate, 0.05 g / L of manganese sulfate tetrahydrate, and the rest is water.The Pediococcus pentosaceus is cultured by using the medium, the bacterial concentration is significantly higher than that of MRS medium, a high-density bacterial body amount is effectively obtained, the utilization rate of industrial by-products is improved, the production cost is greatly reduced, and the production efficiency is improved.

This invention belongs to the field of bioengineering, specifically disclosing a 3-phosphoglycerate dehydrogenase mutant with improved enzyme activity and thermostability. Compared to the wild-type 3-phosphoglycerate dehydrogenase from *Corynebacterium glutamicum*, the mutant contains one mutation from among P85Q, D365Y, A389T, M196V, A183V, and I231V. Enzyme activity assays after induced expression and purification showed that the mutant's enzyme activity was 1.5–3.6 times higher than the wild type, and its thermostability was significantly improved. Therefore, the beneficial mutant provided by this invention lays a good foundation for the industrial fermentation production of L-serine and L-cysteine.

The application discloses a method for producing feed by using glutamic acid isoelectric mother liquor, and belongs to the technical field of industrial fermentation. The technical scheme of the application comprises the following steps: first, the glutamic acid isoelectric mother liquor is subjected to ceramic membrane and ultrafiltration membrane treatment to obtain membrane filtrate and concentrated solution; the concentrated solution is subjected to plate and frame filtration and drying to obtain bacterial protein; the obtained membrane filtrate is subjected to electrodialysis treatment to obtain concentrated water and dilute water; the concentrated water is subjected to evaporation crystallization and solid-liquid separation to obtain ammonium sulfate crystals; and the dilute water is subjected to concentration and drying to obtain feed protein, so that the method for producing feed from the glutamic acid isoelectric mother liquor is realized. The treatment method can increase the yield of feed, improve the yield of ammonium sulfate and bacterial protein, and has great economic and environmental benefits.

The invention relates to a fermentation medium and application thereof in industrial fermentation of animal bifidobacterium subsp. Lactis, the fermentation medium is composed of the following materials by weight: 2-5% of glucose; 1-5% of a yeast extract; 0.1 to 0.5 percent of sodium acetate; 0.05 to 0.2 percent of magnesium sulfate; and the balance of water. Under the condition that the yield and the activity of the bacterial powder are not influenced, the supply chain operation cost and the material purchasing cost are reduced by 20-30%, and meanwhile, the operation error of staff can be reduced; and a technical support is provided for cost reduction and benefit improvement of other probiotics in the industrial field.

The application discloses a kind of recombination of Pseudomonas putida for producing L-lactic acid using whole-component lignocellulose hydrolysate and its construction method and application, belong to the field of metabolic engineering.The application uses Pseudomonas putida KT2440 as chassis, realizes carbon flow to pyruvic acid convergence and prevents product degradation by knocking out aceEF 、 ppsA And lldD Gene;By in-situ replacement, introduce optimized Lactobacillus plantarum ldhL1 Reconfiguration L-lactic acid synthesis pathway;By integration xylD-yjhH Tandem expression cassette enables xylose utilization;And by quorum sensing promoter P Rox306 Overexpression maeB Realize funnel conversion of lignin monomer.The application innovatively uses lignin derivatives in hydrolysate as natural donor of endogenous acetyl-coenzyme A, makes up lethal growth defect caused by knocking out aceEF The engineering bacteria of the application realize the synchronous high-efficiency conversion of glucose, xylose and aromatic monomer under the condition of not adding exogenous auxiliary carbon source and chemical inducer, which greatly reduces the industrial fermentation cost.

The invention discloses a glycosylation-free human enterokinase mutant as well as recombinant expression and application thereof, belongs to the field of genetic engineering and protein technical engineering, and solves the problems of disordered glycosylation modification, low enzymatic activity and low protein expression quantity during recombinant expression of enterokinase in an enterokinase microbiological cell factory. The method comprises the following steps: firstly, carrying out single-site mutation, double-site mutation, three-site mutation and four-site mutation on the basis of a human enterokinase wild type, and constructing a pichia pastoris cell factory to obtain a series of human enterokinase mutants without glycosylation modification. Through calculation design and rational transformation, partial or non-glycosylation is subjected to deep fixed-point transformation, and pichia pastoris cell factory expression is constructed, so that the expression quantity and the total enzyme activity of the mutant are greatly improved. The highest enzyme activity of the glycosylation-free enterokinase mutant generated by fed-batch fermentation in a pichia pastoris cell factory reaches 7.8 * 10 < 4 > U / L, and the enterokinase mutant has the capacity of large-scale industrial fermentation generation and application.

The present invention relates to the field of genetic engineering technology, and in particular to an engineered Escherichia coli strain that produces N-acetylneuraminic acid, as well as its construction method and application. Based on existing engineered bacteria, the present invention further optimizes the metabolic pathway from glucose to N-acetylmannosamine and knocks out the glmM gene to reduce the production of non-related metabolites. The resulting genetically engineered bacteria significantly enhance the Escherichia coli's ability to produce Neu5Ac in fermentation experiments, demonstrating promising prospects for industrial fermentation applications.

The invention relates to the technical field of construction of recombinant bacteria, in particular to recombinant corynebacterium glutamicum for producing L-ornithine as well as a construction method and application of the recombinant corynebacterium glutamicum for producing L-ornithine, corynebacterium glutamicum SO30 is taken as an original strain, a SigD gene and a FruR gene are sequentially knocked out through a suicide plasmid pK18mobsacB mediated homologous recombination technology, and double knockout recombinant corynebacterium glutamicum SO30-SigD-FruR is constructed. The L-ornithine yield of the strain subjected to shake-flask fermentation reaches 51.88 g / L and is increased by 56.7% compared with that of an original strain SO30; the fermentation yield of a 5L bioreactor reaches 123 g / L and is increased by 57.6% compared with that of an original strain SO30, and the cell biomass and the sugar conversion rate are remarkably increased. Through global transcriptional regulation factor transformation, the existing yield bottleneck is broken through, and efficient strains and technical support are provided for industrial fermentation production of L-ornithine.

The invention provides a method for monitoring the synthesis efficiency of pullulan in a fermentation process of aureobasidium pullulans in real time based on a real-time fluorescent quantitative PCR (Polymerase Chain Reaction) technology. A quantitative correlation model of gene expression abundance and polysaccharide yield is established by specifically detecting transcription dynamics of key genes (such as GluK-1, PGM-2, UGT-1 and AmAGS2-2) for synthesizing pullulan polysaccharide. Compared with chemical detection means such as high performance liquid chromatography (HPLC), the method has the advantages of high sensitivity, short time consumption and multi-target parallel analysis, and an innovative molecular tool is provided for intelligent monitoring and yield increase in the industrial fermentation process.

The invention relates to the technical field of microbial engineering and sewage treatment, and discloses a cold-resistant nitrobacterium strain, which is named as Nitrosomonas europaea SZG-AOB-002, is preserved in the China Center for Type Culture Collection (CCTCC), has the preservation number of CCTCC NO: M20232716, is preserved in Wuhan University, Wuhan, China on December 29, 2023, and has the preservation number of CCTCC NO: M20232716. The invention aims to provide an autotrophic pure strain which is clear in source, stable in heredity and capable of realizing efficient ammoxidation in an environment of 8-15 DEG C, and a low-temperature high-density fermentation method adaptive to an industrial fermentation platform is established in a matched manner, so that engineering application of the autotrophic pure strain in low-temperature sewage treatment is realized.

Industrial fermentation is provided for the production of lactic acid from organic waste combined with chemical recycling of polylactic acid, to obtain lactic acid with high yields.

The invention discloses a Yarrowia lipolytica (Yarrowia lipolytica) strain with a wide growth adaptation characteristic and an application of the Yarrowia lipolytica strain in industrial fermentation. The strain is named as Yarrowia lipolytica ML06, is preserved in the China General Microbiological Culture Collection Center (CGMCC), and has the preservation number of CGMCC No. 34889. The invention also discloses a method for preparing the Yarrowia lipolytica ML06 strain. The yarrowia lipolytica ML06 strain can grow under culture conditions of high acid, high temperature and the like, so that the yarrowia lipolytica ML06 strain has important application value in the technical field of industrial biology. The strain can be used for constructing recombinant microorganisms, and can be directly fermented under acidic and high-temperature conditions to produce target chemicals. By means of the characteristic, energy consumption and cost of temperature control and pH adjustment in the production process can be remarkably reduced, the method can directly adapt to the accumulation environment of some acid products in the production process, and therefore the technological process is simplified, and the production efficiency is improved.

The invention discloses pichia pastoris and application thereof, and belongs to the field of microbial fermentation. According to the present invention, the lactic acid producing type pichia pastoris special for companies is subjected to mutagenesis through the ARTP mutagenesis technology, such that the pichia pastoris mutant strain Pichia pastoris PLS006 capable of maintaining the lactic acid producing performance under the low ventilation condition (0.33 vvm) is obtained, and the preservation number is CCTCC M 20251051; the fermentation ventilation quantity of the strain is remarkably reduced to 0.33 vvm from 1 vvm in the traditional process, the energy consumption is reduced, meanwhile, the lactic acid production efficiency is not remarkably reduced, and the strain is suitable for industrial fermentation production. The invention also provides application of the strain in the fields of lactic acid preparation, food fermentation and biosynthesis.

The invention relates to cigar core raw material manufacturing, in particular to a production process suitable for manufacturing a machine-made cigar core raw material from cigar tobacco leaves. The production process sequentially comprises the following working procedures: industrially fermenting cigar tobacco leaves, airing and drying, moistening the cigar tobacco leaves with hot air, threshing and removing stems, sorting tobacco flakes, screening large tobacco flakes, removing impurities, drying the tobacco flakes, perfuming the tobacco flakes, and storing the tobacco flakes to obtain a raw material suitable for the machine-made cigar core. By referring to the flue-cured tobacco threshing and redrying technological process and combining the latest research result of the industry on industrial fermentation, the complete and feasible technological process and control technology for cigar core tobacco production are provided for solving the problem of production of the machine-made cigar core tobacco, the processing technological level and sensory quality of the machine-made cigar core tobacco are further improved by adopting the process, and the production cost is reduced. On the basis of ensuring processing economic indexes, the processing quality of the machine-made cigar core raw material can be effectively controlled, and the guarantee level of the machine-made cigar raw material is improved.

The invention discloses a method for synchronously improving the quality uniformity and the aroma content of a dark cigar coat, and belongs to the technical field of tobaccos, and the method specifically comprises the following steps: carrying out moisture regaining treatment on part or all cigar tobacco leaves by using moisture regaining water containing ethanol, and stacking and fermenting the moisture regained tobacco leaves. According to the method, the color uniformity and the aroma yield of the obtained tobacco leaves can be efficiently and remarkably improved only by adding a proper amount of ethyl alcohol in the cigar tobacco leaf fermentation process, and the method has the advantages of being easy and convenient to operate, low in cost, high in safety and the like and is suitable for industrial fermentation production of cigar tobacco leaves.

This application discloses the application of vitamin D2 in the preparation of a heat-resistant protective agent for the fermentation industry. This application's research found that VD2 treatment can significantly improve the performance of *Saccharomyces cerevisiae* under heat stress conditions. S. yeast This invention improves the growth status of *Saccharomyces cerevisiae*, significantly increases glutathione peroxidase (GPx) activity, and reduces cell membrane damage. It boasts advantages such as low cost, high safety, simple operation, and wide applicability, and can be widely applied to high-temperature fermentation systems of *Saccharomyces cerevisiae* in the food and pharmaceutical industries. It effectively solves the problem of high-temperature stress in industrial fermentation and has significant industrial application value.

The present application relates to the technical field of bioengineering, and particularly relates to a Kluyveromyces marxianus strain and application thereof in fermentation production of ethanol. The present application provides a Kluyveromyces marxianus strain huJ01, and the preservation number of the strain is CGMCC No.22931. The strain can still maintain good growth and ethanol synthesis capacity under stress conditions such as high temperature, high acid, high ethanol and high salt. Under the optimal conditions of inulinase activity, the ethanol yield can reach 2.41%vol after 20% inoculation and static culture at 60 DEG C for 120 h. The strain is inoculated at 4% in a high stress culture medium containing 11.4% ethanol, pH 3.5 and 3.51% NaCl, and is statically cultured at 40 DEG C for 12 h, and the concentration of the bacterial liquid is significantly increased or not significantly decreased. It is shown that the strain still has strong ethanol fermentation performance under extreme environment, and is suitable for special industrial fermentation scenes such as high salt, high acid and high ethanol.

The present invention relates to microorganisms, materials and methods for improving the activity of microorganisms. In particular, the present invention provides methods for preparing microorganisms to improve metabolic activity and / or synergy, metabolism-activated and / or synergistic microorganisms, and methods of using these metabolism-activated and / or synergistic microorganisms, particularly in industrial fermentation processes.

The present invention relates to the field of industrial fermentation and protein production. In particular, it relates to a method for producing a protein of interest in a fermentation medium comprising the following steps a) inoculating a fermentation medium with a Bacillus host cell comprising a gene encoding a protein of interest under the control of a promoter; b) cultivating the Bacillus host cell in the fermentation medium under conditions conducive for the growth of the Bacillus host cell and the expression of the protein of interest, c) adding sulfate to the fermentation medium to reach a concentration of at least 20 mM of sulfate in the fermentation medium; and d) allowing the protein of interest to precipitate and / or crystallize during cultivation; wherein the fermentation medium comprises an amino acid derivative in an amount of 0-30 g / l of fermentation medium. Further contemplated is the use of a combination of a sulfate and an amino acid derivative for producing a protein of interest in Bacillus host cell in a fermentation medium and a crystallized protein of interest obtained by or obtainable by the method of the invention.

The application discloses a kind of for screening of key regulatory factor of acid resistance of corynebacterium glutamicum resolving method and application, belong to the cross field of microbial genetic regulation, bioinformation calculation and industrial fermentation engineering.The resolving method for screening of key regulatory factor of acid resistance of corynebacterium glutamicum provided, including: the strain expression data of corynebacterium glutamicum under different fermentation acidity conditions are arranged;Initial candidate relationship framework model is constructed;Directional flow characteristic kernel function under the condition of multi-condition expression mode is constructed, and is combined with initial candidate relationship framework model, to infer the directionality influence intensity;The stability of the obtained directionality relationship is verified;Possible direction determination and link rationality check are carried out, and then the acid resistance regulation link and key gene are output.The present application can provide a kind of high reliability calculation means for the acid resistance improvement and precise positioning of key regulatory factor of industrial fermentation strain.