40 results about "Industrial fermentation" patented technology

The invention provides a fermentation device based on multi-physical field coordination and application thereof. The fermentation device comprises a fermentation tank unit, an annular magnetic field generation unit, a pulsed electric field generation unit and a control unit. The stable and energy-free background magnetic field is provided through the permanent magnet array, the background magnetic field and the pulsed electric field form synergy, the metabolism efficiency of microorganisms is remarkably improved, the operation cost is low, the device structure is simple, the device can be flexibly deployed, and the device is suitable for large-scale deployment and transformation on an industrial fermentation tank needing long-term and stable operation; a static background magnetic field generated by the permanent magnet is highly stable, is not influenced by fluctuation of a power grid, and is mutually independent and non-interfering with the distribution of a pulse electric field, so that the reliability and repeatability of the process are ensured.

The application discloses a kind of genetic engineering escherichia coli plasmid expression culture medium and fermentation process, including basic medium and feed medium;The basic medium includes glycerol, yeast hydrolysate, yeast peptone, L-glutamic acid, L-valine, L-isoleucine, arginine, tyrosine, lysine, proline, glycine, ammonium sulfate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, citric acid, magnesium sulfate, ferrous sulfate, zinc sulfate, calcium chloride, manganese chloride, cobalt chloride, copper chloride, sodium ethylenediaminetetraacetate;Feed medium includes glycerol, citric acid, magnesium sulfate, ferrous sulfate, zinc sulfate, calcium chloride, manganese chloride, cobalt chloride, copper chloride, sodium ethylenediaminetetraacetate.The application inhibits the accumulation of harmful metabolites, and creates the optimal intracellular environment for stable replication of plasmid.In industrial fermentation scale, the amount of plasmid harvested per liter of fermentation broth is more than 1 gram, and the supercoiling ratio of plasmid DNA is stably maintained at more than 90%, with minimal batch-to-batch variation.

The invention provides a method and a kit for detecting the content of grease in yeast. The method for detecting the content of grease in yeast comprises the following steps: S1, preparing a sodium chloride solution with a series of concentration gradients; s2, sequentially adding bacterial liquid containing yeast to be detected into the sodium chloride solution, uniformly mixing, then carrying out centrifugal treatment, and after the centrifugal treatment, observing the initial suspended salinity, the middle suspended salinity and the complete suspended salinity of the yeast in the sodium chloride solution; s3, determining the grease content in the to-be-detected yeast according to the initial suspension salinity, the middle suspension salinity and the complete suspension salinity. According to the method for detecting the content of the grease in the yeast cells by using the saline water with different concentrations, the range of the content of the grease in the yeast cells, which can be detected, is about 10%-60%, the detection precision is about 10%, and the method has important significance on rapid detection of the content of the grease in industrial fermentation of the yeast.

This invention belongs to the field of microbial metabolic engineering and synthetic biology, specifically relating to a strain for high-yield... R,R -2,3-Butanediol ( R,R Salt-tolerant Jeju Bacillus DT01 IM3 DLB6 (-2,3-BDO), production strain constructed from this strain and its application in fermentation production. R,R Applications of -2,3-BDO. This invention screens a high-yielding strain through heavy ion beam irradiation mutagenesis. R,R Jeju Bacillus DT01 IM3 DL B6 with -2,3-BDO, preservation number CGMCC No. 37311. This strain exhibits excellent salt tolerance and low endotoxin properties. The production strain constructed from it has extremely high product synthesis capabilities, enabling high-concentration, high-intensity industrial fermentation production.

The invention provides a sensor-based cordyceps militaris fermented beverage fermentation evaluation method and system, and relates to the technical field of fermentation engineering.Multi-frequency ultrasonic signals are transmitted and received through an ultrasonic transceiver array deployed on the outer wall of a fermentation tank; and reconstructing the signal into an acoustic parameter three-dimensional distribution image of the fermentation liquid in the fermentation tank by utilizing a tomography algorithm. And based on a pre-established mapping relation, mapping the acoustic parameter three-dimensional distribution image into three-dimensional distribution of the fermentation state parameters. And finally, based on the three-dimensional distribution of the fermentation state parameters, evaluating the overall real state of the internal environment of the fermentation tank to obtain spatial non-uniformity quantitative data of the internal environment of the fermentation tank, and generating an instruction for regulating and controlling the fermentation process according to the data. The method effectively solves the problem that monitoring of a traditional local sensor is not accurate due to non-uniformity of the internal environment of a large industrial fermentation tank in the prior art.

The application discloses a recombinant Escherichia coli for efficiently expressing dsRNA, which is a TG1 strain with gene defects and into which the following plasmids are introduced: a first plasmid comprising a T7 RNA polymerase expression module; and a second plasmid comprising a dsRNA expression module. rnc The application also discloses a method for producing dsRNA, and application of the recombinant Escherichia coli in preparing nucleic acid pesticides. rnc The application provides a new recombinant Escherichia coli with gene defects for producing dsRNA in the field of nucleic acid pesticides. rnc The knockout has no significant influence on the growth rate of the recombinant Escherichia coli, and the recombinant Escherichia coli has great industrial fermentation potential. The recombinant Escherichia coli adopts a double-plasmid expression system, and the dsRNA expression module structure with double T7 terminators is used to improve the expression amount and purity of dsRNA synthesis.

The invention provides a method for producing propionic acid and calcium salt thereof through fermentation, and belongs to the technical field of agricultural microbial fermentation. The method for producing propionic acid by fermentation comprises the following steps: (1) activating acid-producing propionibacterium with the preservation number of CGMCC1.2230, inoculating the acid-producing propionibacterium to a special seed culture medium for propionic acid, and standing and culturing for 48-72 hours under the anaerobic condition at the temperature of 37 + / -0.5 DEG C; and (2) inoculating the obtained bacterial liquid into a fermentation medium according to an inoculation ratio of 2.8-3.2%, fermenting under an anaerobic condition at 37 + / -0.5 DEG C and a stirring speed of 80-100rpm until the content of propionic acid is not obviously changed, and adjusting the pH value by using a calcium hydroxide suspension with a solid-to-liquid ratio of 15-20% during fermentation. According to the method, the yield of propionic acid produced by fermentation of the propionibacterium propionate is increased by adjusting the proportion of fermentation substrates and controlling the fermentation pH in a segmented mode, the yield of propionic acid produced by fermentation of the propionibacterium propionate can reach 16.23 g / L, and the production intensity is 0.225 gL <-1 > h <-1 >. The mixed fermentation ensures the realization of high-titer propionic acid production, and provides a theoretical basis for increasing the yield of propionic acid produced by industrial fermentation to a certain extent.

This invention belongs to the field of bioengineering, specifically disclosing a 3-phosphoglycerate dehydrogenase mutant with improved enzyme activity and thermostability. Compared to the wild-type 3-phosphoglycerate dehydrogenase from *Corynebacterium glutamicum*, the mutant contains one mutation from among P85Q, D365Y, A389T, M196V, A183V, and I231V. Enzyme activity assays after induced expression and purification showed that the mutant's enzyme activity was 1.5–3.6 times higher than the wild type, and its thermostability was significantly improved. Therefore, the beneficial mutant provided by this invention lays a good foundation for the industrial fermentation production of L-serine and L-cysteine.

The application discloses a method for producing feed by using glutamic acid isoelectric mother liquor, and belongs to the technical field of industrial fermentation. The technical scheme of the application comprises the following steps: first, the glutamic acid isoelectric mother liquor is subjected to ceramic membrane and ultrafiltration membrane treatment to obtain membrane filtrate and concentrated solution; the concentrated solution is subjected to plate and frame filtration and drying to obtain bacterial protein; the obtained membrane filtrate is subjected to electrodialysis treatment to obtain concentrated water and dilute water; the concentrated water is subjected to evaporation crystallization and solid-liquid separation to obtain ammonium sulfate crystals; and the dilute water is subjected to concentration and drying to obtain feed protein, so that the method for producing feed from the glutamic acid isoelectric mother liquor is realized. The treatment method can increase the yield of feed, improve the yield of ammonium sulfate and bacterial protein, and has great economic and environmental benefits.

The invention relates to a fermentation medium and application thereof in industrial fermentation of animal bifidobacterium subsp. Lactis, the fermentation medium is composed of the following materials by weight: 2-5% of glucose; 1-5% of a yeast extract; 0.1 to 0.5 percent of sodium acetate; 0.05 to 0.2 percent of magnesium sulfate; and the balance of water. Under the condition that the yield and the activity of the bacterial powder are not influenced, the supply chain operation cost and the material purchasing cost are reduced by 20-30%, and meanwhile, the operation error of staff can be reduced; and a technical support is provided for cost reduction and benefit improvement of other probiotics in the industrial field.

The application discloses a kind of recombination of Pseudomonas putida for producing L-lactic acid using whole-component lignocellulose hydrolysate and its construction method and application, belong to the field of metabolic engineering.The application uses Pseudomonas putida KT2440 as chassis, realizes carbon flow to pyruvic acid convergence and prevents product degradation by knocking out aceEF 、 ppsA And lldD Gene;By in-situ replacement, introduce optimized Lactobacillus plantarum ldhL1 Reconfiguration L-lactic acid synthesis pathway;By integration xylD-yjhH Tandem expression cassette enables xylose utilization;And by quorum sensing promoter P Rox306 Overexpression maeB Realize funnel conversion of lignin monomer.The application innovatively uses lignin derivatives in hydrolysate as natural donor of endogenous acetyl-coenzyme A, makes up lethal growth defect caused by knocking out aceEF The engineering bacteria of the application realize the synchronous high-efficiency conversion of glucose, xylose and aromatic monomer under the condition of not adding exogenous auxiliary carbon source and chemical inducer, which greatly reduces the industrial fermentation cost.

The invention relates to the technical field of construction of recombinant bacteria, in particular to recombinant corynebacterium glutamicum for producing L-ornithine as well as a construction method and application of the recombinant corynebacterium glutamicum for producing L-ornithine, corynebacterium glutamicum SO30 is taken as an original strain, a SigD gene and a FruR gene are sequentially knocked out through a suicide plasmid pK18mobsacB mediated homologous recombination technology, and double knockout recombinant corynebacterium glutamicum SO30-SigD-FruR is constructed. The L-ornithine yield of the strain subjected to shake-flask fermentation reaches 51.88 g / L and is increased by 56.7% compared with that of an original strain SO30; the fermentation yield of a 5L bioreactor reaches 123 g / L and is increased by 57.6% compared with that of an original strain SO30, and the cell biomass and the sugar conversion rate are remarkably increased. Through global transcriptional regulation factor transformation, the existing yield bottleneck is broken through, and efficient strains and technical support are provided for industrial fermentation production of L-ornithine.

Industrial fermentation is provided for the production of lactic acid from organic waste combined with chemical recycling of polylactic acid, to obtain lactic acid with high yields.

The invention discloses a Yarrowia lipolytica (Yarrowia lipolytica) strain with a wide growth adaptation characteristic and an application of the Yarrowia lipolytica strain in industrial fermentation. The strain is named as Yarrowia lipolytica ML06, is preserved in the China General Microbiological Culture Collection Center (CGMCC), and has the preservation number of CGMCC No. 34889. The invention also discloses a method for preparing the Yarrowia lipolytica ML06 strain. The yarrowia lipolytica ML06 strain can grow under culture conditions of high acid, high temperature and the like, so that the yarrowia lipolytica ML06 strain has important application value in the technical field of industrial biology. The strain can be used for constructing recombinant microorganisms, and can be directly fermented under acidic and high-temperature conditions to produce target chemicals. By means of the characteristic, energy consumption and cost of temperature control and pH adjustment in the production process can be remarkably reduced, the method can directly adapt to the accumulation environment of some acid products in the production process, and therefore the technological process is simplified, and the production efficiency is improved.

The invention discloses a method for synchronously improving the quality uniformity and the aroma content of a dark cigar coat, and belongs to the technical field of tobaccos, and the method specifically comprises the following steps: carrying out moisture regaining treatment on part or all cigar tobacco leaves by using moisture regaining water containing ethanol, and stacking and fermenting the moisture regained tobacco leaves. According to the method, the color uniformity and the aroma yield of the obtained tobacco leaves can be efficiently and remarkably improved only by adding a proper amount of ethyl alcohol in the cigar tobacco leaf fermentation process, and the method has the advantages of being easy and convenient to operate, low in cost, high in safety and the like and is suitable for industrial fermentation production of cigar tobacco leaves.

This application discloses the application of vitamin D2 in the preparation of a heat-resistant protective agent for the fermentation industry. This application's research found that VD2 treatment can significantly improve the performance of *Saccharomyces cerevisiae* under heat stress conditions. S. yeast This invention improves the growth status of *Saccharomyces cerevisiae*, significantly increases glutathione peroxidase (GPx) activity, and reduces cell membrane damage. It boasts advantages such as low cost, high safety, simple operation, and wide applicability, and can be widely applied to high-temperature fermentation systems of *Saccharomyces cerevisiae* in the food and pharmaceutical industries. It effectively solves the problem of high-temperature stress in industrial fermentation and has significant industrial application value.

The present application relates to the technical field of bioengineering, and particularly relates to a Kluyveromyces marxianus strain and application thereof in fermentation production of ethanol. The present application provides a Kluyveromyces marxianus strain huJ01, and the preservation number of the strain is CGMCC No.22931. The strain can still maintain good growth and ethanol synthesis capacity under stress conditions such as high temperature, high acid, high ethanol and high salt. Under the optimal conditions of inulinase activity, the ethanol yield can reach 2.41%vol after 20% inoculation and static culture at 60 DEG C for 120 h. The strain is inoculated at 4% in a high stress culture medium containing 11.4% ethanol, pH 3.5 and 3.51% NaCl, and is statically cultured at 40 DEG C for 12 h, and the concentration of the bacterial liquid is significantly increased or not significantly decreased. It is shown that the strain still has strong ethanol fermentation performance under extreme environment, and is suitable for special industrial fermentation scenes such as high salt, high acid and high ethanol.

The present invention relates to the field of industrial fermentation and protein production. In particular, it relates to a method for producing a protein of interest in a fermentation medium comprising the following steps a) inoculating a fermentation medium with a Bacillus host cell comprising a gene encoding a protein of interest under the control of a promoter; b) cultivating the Bacillus host cell in the fermentation medium under conditions conducive for the growth of the Bacillus host cell and the expression of the protein of interest, c) adding sulfate to the fermentation medium to reach a concentration of at least 20 mM of sulfate in the fermentation medium; and d) allowing the protein of interest to precipitate and / or crystallize during cultivation; wherein the fermentation medium comprises an amino acid derivative in an amount of 0-30 g / l of fermentation medium. Further contemplated is the use of a combination of a sulfate and an amino acid derivative for producing a protein of interest in Bacillus host cell in a fermentation medium and a crystallized protein of interest obtained by or obtainable by the method of the invention.

The application discloses a kind of for screening of key regulatory factor of acid resistance of corynebacterium glutamicum resolving method and application, belong to the cross field of microbial genetic regulation, bioinformation calculation and industrial fermentation engineering.The resolving method for screening of key regulatory factor of acid resistance of corynebacterium glutamicum provided, including: the strain expression data of corynebacterium glutamicum under different fermentation acidity conditions are arranged;Initial candidate relationship framework model is constructed;Directional flow characteristic kernel function under the condition of multi-condition expression mode is constructed, and is combined with initial candidate relationship framework model, to infer the directionality influence intensity;The stability of the obtained directionality relationship is verified;Possible direction determination and link rationality check are carried out, and then the acid resistance regulation link and key gene are output.The present application can provide a kind of high reliability calculation means for the acid resistance improvement and precise positioning of key regulatory factor of industrial fermentation strain.

The present invention relates to the field of industrial fermentation. In particular, it relates to method for producing a protein of interest from a Bacillus host cell comprising the steps of inoculating a fermentation medium with a Bacillus host cell comprising an expression construct for a gene encoding a protein of interest, cultivating the Bacillus host cell in said fermentation medium under conditions conducive for the growth of the Bacillus host cell and the expression of the protein of interest for a cultivation phase, wherein the cultivation of the Bacillus host cell during said cultivation phase comprises the addition of at least one feed solution and wherein the at least one feed solution provides a carbon source, and separating portions from said Bacillus host cell culture during the cultivation phase at different time points and obtaining the protein of interest from said portions.

The invention relates to a kit and a method for growing or fermenting oxygen-sensitive microorganisms with non-zero residual dissolved oxygen. The kit comprises a heat-stable, heat-sterilisable part A, having at least one source of nitrogen and / or peptides and at least one source of carbon; and a thermolabile part B, which can be added aseptically after cooling, providing a biomimetic carbohydrate block with an N-acetylhexosamine motif and additional components selected from fucosylated, sialylated, branched and / or sulphated structures. The method reconstitutes the medium by combining A and B, inoculates the microorganism and operates in a closed vessel without continuous purging of inert gas, maintaining dissolved oxygen below air saturation and, during part of the process, above zero and up to five milligrams per litre, in order to obtain viable biomass and / or cell-free supernatant for industrial fermentation.

The present invention relates to the field of industrial fermentation and protein production. In particular, it relates to a method for producing a protein of interest in a fermentation medium comprising the following steps a) inoculating a fermentation medium with a Bacillus host cell comprising a gene encoding a protein of interest under the control of a promoter; b) cultivating the Bacillus host cell in the fermentation medium under conditions conducive for the growth of the Bacillus host cell and the expression of the protein of interest, c) adding sulfate to the fermentation medium to reach a concentration of at least 20 mM of sulfate in the fermentation medium; and d) allowing the protein of interest to precipitate and / or crystallize during cultivation. Further contemplated is the use of a high sulfate concentration in a fermentation medium for producing a protein of interest in a Bacillus host cell and a crystallized protein of interest obtained by or obtainable by the method of the invention.

The invention discloses a fermentation production method and an extraction method of streptomyces parvus SJS342 and actinomycin D, and relates to the technical field of microorganisms. The classification name of the streptomyces parvulus is streptomyces parvulus, and the preservation number of the streptomyces parvulus is CGMCC (China General Microbiological Culture Collection Center) No.36775. The streptomyces parvulus SJS342 is named as streptomyces parvulus. The novel streptomyces parvus SJS342 strain provided by the invention can be used for efficiently fermenting actinomycin D, the yield of actinomycin D is greatly improved, the stability of the fermentation yield is good, and the streptomyces parvus SJS342 strain can be applied to industrial fermentation production. In addition, high-purity actinomycin D and a high-activity pure actinomycin D product can be obtained by extracting the fermentation liquor, and the purity requirements of medicine research and development or industrial production are met.

The invention discloses a portable microorganism growth curve analyzer, and belongs to the technical field of microorganism detection. A detection module of the analyzer adopts a multi-path wavelength-selectable LED light source and photodiode correlation type structure, is used for realizing real-time optical density detection of microorganism samples in a 6-pore plate and a 12-pore plate, and can automatically draw a growth curve; the X-axis moving module is composed of a sliding block structure driven by a stepping motor, and automatic scanning of multi-channel samples is achieved. The communication module supports Bluetooth and a USB interface, and can realize upper computer control and data export. According to the invention, high-precision, multi-channel and automatic microbial growth curve detection can be realized under a miniaturized structure, and the operation is simple and convenient; the method is suitable for various application scenes such as clinical medical examination, drug research and development, environment monitoring, food safety, water quality detection, pathogen monitoring of agriculture and animal husbandry, industrial fermentation and biological processing, public health monitoring, teaching and scientific research and the like.

The invention discloses saccharomyces cerevisiae capable of efficiently utilizing acetic acid and application of the saccharomyces cerevisiae in production of terpenoids, and belongs to the technical field of synthetic biology and industrial microbiology. The Saccharomyces cerevisiae is Saccharomyces cerevisiae SF13, and the preservation number of the Saccharomyces cerevisiae is GDMCC No: 67119. According to the invention, an evolved strain Saccharomyces cerevisiae SF13 is obtained through adaptive evolution, the evolved strain shows that saccharomyces cerevisiae can grow under the condition of taking acetic acid and salt thereof as a unique carbon source, and the retardation period is shortened; acetic acid and salt thereof can be efficiently and rapidly utilized, the genetic stability is good, the yield character is stable, and the value is high when the method is applied to industrial fermentation. The strain can greatly improve the yield and carbon conversion rate of target terpenoids, has sufficient acetyl coenzyme A precursor supply, and is an excellent terpenoid production strain.

The invention relates to the technical field of microorganisms, in particular to pichia kudriavzevii SJTU-BEL-1 capable of producing single-cell protein and application of the pichia kudriavzevii SJTU-BEL-1. The pichia kudriavzevii SJTU-BEL-1 provided by the invention is a strain obtained by performing primary screening, secondary screening and identification on supermarket over-ripe strawberry residues, and when the pichia kudriavzevii SJTU-BEL-1 is cultured for 72 hours, the dry weight of thalli reaches 41.25 + / -0.69 g / L, which is obviously superior to that of other strains. The strain has great application potential in single-cell protein production, and can still maintain high-yield protein capacity under the condition of high-density fermentation; the total protein content and the total amino acid content are higher than those of a common yeast fermentation product, and good industrial fermentation potential and application prospects are achieved.

The present application relates to the field of biotechnology, and particularly relates to a method for improving fermentation level of Bacillus subtilis oxalate decarboxylase based on SigA transcription switch. The present application is aimed at RNA polymerase housekeeping sigma factor SigA, and a transcription switch capable of accurately regulating expression level of the same is designed. The transcription switch is applied to fermentation of Bacillus subtilis oxalate decarboxylase, and oxalate decarboxylase can be stably and efficiently synthesized, so that the induction expression efficiency of oxalate decarboxylase is increased by 10 times compared with wild type strain. 3 -10 4 The present application provides a new design idea for efficient regulation of gene expression in the field of Bacillus subtilis metabolic engineering and synthetic biology, and the method for improving fermentation level of Bacillus subtilis oxalate decarboxylase has important application prospects in the fields of industrial fermentation of oxalate decarboxylase, degradation of industrial wastewater or oxalic acid pollution in agricultural product processing, degradation of oxalic acid pollution in soil and preparation of microbial preparation for degrading oxalic acid.

The application provides a recombinant bacillus subtilis producing phage lytic enzyme LysJPP58 and a construction method and application thereof. The recombinant bacillus subtilis integrates the lytic enzyme LysJPP58 into the genome of a host bacterium bacillus subtilis, wherein the lytic enzyme LysJPP58 is derived from a staphylococcus aureus phage, and an amino acid sequence of the lytic enzyme LysJPP58 is shown as SEQ ID NO. 1. The recombinant bacillus subtilis can effectively secrete the LysJPP58 protein with lytic activity, and can significantly lyse staphylococcus aureus. The recombinant bacillus subtilis has the characteristics of safety, sustainability and industrial fermentation production, and provides a new technical approach for the development of animal health, food safety and medical antibacterial preparations.

The invention belongs to the technical field of microbial genetic engineering and industrial fermentation, and discloses an aspergillus niger engineering strain for producing L-malic acid by using xylose and a construction method and application of the aspergillus niger engineering strain. A three-gene recombination strain of an XR gene for coding xylose reductase, an XDH gene for coding xylitol dehydrogenase and an XK gene for coding xylulokinase through simultaneous overexpression is obtained through directional construction. Through synergistic overexpression of the XR gene, the XDH gene and the XK gene, the limitation that the starting strain LH-1 cannot utilize the xylose is successfully broken through, and effective utilization and directional conversion of the xylose are realized. The strain can be used for producing L-malic acid by using a low-cost xylose raw material, so that the production cost is remarkably reduced, a new technical path is provided for industrial production of organic acid, and the strain has a wide industrial application prospect.

The invention provides a method for simulating influence of industrial-scale dissolved carbon dioxide on anaerobic fermentation of saccharomyces cerevisiae, and belongs to the technical field of fermentation engineering. The invention provides three simulation methods: a DCO2 steady state simulation method comprises the following steps: carrying out anaerobic constant culture of saccharomyces cerevisiae in a bioreactor, adjusting the gas inlet ratio of N2 to CO2 to control the concentration of DCO2 at a target concentration and keep the concentration stable, and monitoring the change of metabolic indexes in real time; according to the DCO2 dynamic change simulation method, the concentration of DCO2 is changed according to a preset rule through program control, and metabolism reversibility is evaluated; according to the DCO2 periodic fluctuation simulation method, a high / low DCO2 double-chamber reactor system is constructed, and fermentation liquor is periodically mixed by setting circulation time. According to the method, the steady-state high concentration, dynamic change and periodic fluctuation characteristics of the DCO2 in the industrial reactor can be systematically reproduced under the laboratory scale, and an effective means is provided for evaluating the influence of the DCO2 on saccharomyces cerevisiae metabolism and the industrial fermentation amplification effect.