121 results about "Industrial fermentation" patented technology

The invention discloses a method for improving growth of corynebacterium glutamicum under high-temperature stress, and aims to overcome the problems of abnormal growth and metabolism, even death and the like caused by temperature rise of existing microorganisms in an industrial fermentation production process, adaptive laboratory evolution is adopted, corynebacterium glutamicum ATCC 13032 is used as an original strain, and the corynebacterium glutamicum ATCC 13032 is used as an original strain. And carrying out heat stress subculture for 42.9 days, and separating out the strains HES1 and HES2 with improved high temperature resistance. The whole genome is re-sequenced to obtain mutant elements cspB and gltA which enable the strain to generate high-temperature adaptive change. And carrying out high-temperature test on the abrupt change element. In specific implementation, the two mutants are respectively overexpressed, and in a test, compared with a control strain WT, the 42-DEG C high-temperature tolerance is respectively improved by about 14.4% and 7%. Therefore, the beneficial mutant obtained through adaptive laboratory evolution lays a good foundation for application of corynebacterium glutamicum in industrial high-temperature conditions.

The invention discloses propionibacterium capable of obtaining high-yield propionic acid through compound mutation screening and a method for fermenting propionic acid by using the propionibacterium. The high-yield propionic acid strain YCBS010 (CGMCC No.31160) is obtained by carrying out compound mutagenesis on acid-producing propionibacterium CGMCC 1.2232 serving as an original strain through microwave-ultrasonic combined mutagenesis and compound normal-pressure and room-temperature plasma (ARTP) mutagenesis means. According to the invention, YPA15 is used as a starting strain, microwave and ultrasonic combined mutagenesis is carried out for 100 seconds to screen a bacterial strain with increased propionic acid yield, then ARTP mutagenesis is carried out for 90 seconds to screen out a high-yield mutant strain YCBS010, fermentation in a 1-ton tank verifies that the highest propionic acid yield reaches 65.87 g / L and the fermentation intensity is 0.73 g / L.h after fermentation is carried out for 90 hours, and the bacterial strain has better industrial fermentation potential.

The invention discloses bacillus capable of tolerating various organic solvents and application of the bacillus. The invention provides a bacillus sp. YL23 strain, and the preservation number of the bacillus sp. YL23 strain is CGMCC (China General Microbiological Culture Collection Center) NO.29579. Also provided is the use thereof in the preparation of recombinant microorganisms for industrial fermentation. YL23 has potential application value in the technical field of industrial biology, including that substitute raw materials such as xylose can be utilized to ferment and produce target chemicals under acidic and high-temperature conditions. The characteristic can realize high-temperature production in the field of fermentation, and the processes of sterilization and the like are reduced. Meanwhile, the acid fermentation condition and the good organic solvent tolerance characteristic are beneficial to accumulation of target products such as organic acid.

This invention discloses a *Propionibacterium japonicum* strain selected through space-based combined chemical mutagenesis and its mutagenesis, screening, and propionic acid fermentation methods. Using *Propionibacterium japonicum* strain CICC22741 from the China Industrial Microbiological Culture Collection Center as the starting strain, this invention employs space-based mutagenesis via the "Shijian-19" satellite combined with ethyl methanesulfonate chemical mutagenesis. After two rounds of mutagenesis screening, a high-propionic acid-producing strain, *Propionibacterium japonicum* DJ04-16, was obtained. This strain was deposited at the China General Microbiological Culture Collection Center on June 16, 2025, with accession number CGMCC No. 34914. Subsequently, the propionic acid fermentation conditions of *Propionibacterium japonicum* DJ04-16 were optimized. Validation was performed in a 1-ton fermentation tank. After 96 hours of fermentation, the highest propionic acid yield reached 67.36 g / L, with a fermentation intensity of 0.702 g / L / h. This strain exhibits good industrial fermentation potential.

The invention discloses an Escherichia coli recombinant bacterium capable of efficiently expressing dsRNA, the Escherichia coli recombinant bacterium is an rnc gene defect type TG1 strain, and the following plasmids are transferred: a first plasmid comprising a T7 RNA polymerase expression module, a second plasmid comprising a dsRNA polymerase expression module, a third plasmid comprising a dsRNA polymerase expression module, and a fourth plasmid comprising a dsRNA polymerase expression module; and a second plasmid comprising a dsRNA expression module. The invention also discloses a method for producing the dsRNA. The invention also discloses an application of the recombinant escherichia coli in preparation of nucleic acid pesticides. The invention provides a novel rnc gene defect type escherichia coli recombinant bacterium for dsRNA production in the field of nucleic acid pesticides. The rnc knockout of the strain has no obvious influence on the growth rate of the strain, and the strain has great industrial fermentation potential. According to the recombinant bacterium, a double-plasmid expression system is adopted, and meanwhile, the dsRNA expression module structure of double T7 terminators is utilized to optimize and improve the expression quantity and purity of dsRNA synthesis.

The present invention discloses a method for predicting penicillin concentration based on local migration modeling, belonging to the field of soft sensor modeling and application for industrial fermentation production processes. The method divides source and target domain data into subdomains for the penicillin fermentation process. A partial least squares model is used to identify the sub-source domain with the highest correlation with each sub-target domain. A refined migration between subdomains is constructed based on migration component analysis, minimizing the loss of local data information during the migration process. Finally, a prediction model is established within each subdomain, and the target domain data is used to predict penicillin concentration using the established model, thereby improving prediction accuracy.

The present disclosure provides compositions and methods of recovering a policosanol or a sterol from a mixture comprising one or more fatty alcohols, aldehydes, fatty acids, and / or sterols. The mixture may be, for example, a lipid extract obtained from industrial fermentation processes (e.g., filter cake, distillate residue). The policosanol and / or sterol fraction recovered may be enriched in certain fatty alcohols (e.g., 1-octacosanol, 1-triacontanol, and / or 1-dotriacontanol), or enriched in certain sterols (e.g., ergosterol, campesterol, stigmasterol, and / or β-sitosterol). These bioactive molecules may be useful in the treatment or prevention of various metabolic conditions (e.g., obesity, dyslipidemia, cardiovascular conditions, and other chronic metabolic diseases).

The invention belongs to the technical field of synthetic biology and enzyme engineering, and particularly relates to a flavone 4 '-O-methyltransferase mutant as well as a preparation method and application thereof. Specifically, wild type methyltransferase as shown in SEQ ID NO.1 is subjected to mutation modification, and a series of mutants with improved enzyme activity are obtained. The mutant enzyme is obtained by respectively performing point mutation and superposition mutation on a plurality of amino acid sites of a wild-type methyltransferase amino acid sequence, so that the capability of synthesizing icaritin by the methyltransferase is remarkably enhanced, and the highest yield is 11.4 times of that of the wild-type methyltransferase. Certain support is provided for industrial fermentation production of icaritin, and therefore the icaritin has good practical application value.

The invention provides a fermentation device based on multi-physical field coordination and application thereof. The fermentation device comprises a fermentation tank unit, an annular magnetic field generation unit, a pulsed electric field generation unit and a control unit. The stable and energy-free background magnetic field is provided through the permanent magnet array, the background magnetic field and the pulsed electric field form synergy, the metabolism efficiency of microorganisms is remarkably improved, the operation cost is low, the device structure is simple, the device can be flexibly deployed, and the device is suitable for large-scale deployment and transformation on an industrial fermentation tank needing long-term and stable operation; a static background magnetic field generated by the permanent magnet is highly stable, is not influenced by fluctuation of a power grid, and is mutually independent and non-interfering with the distribution of a pulse electric field, so that the reliability and repeatability of the process are ensured.

The invention belongs to the technical field of microorganisms, a streptococcus zooepidemicus mutant strain CGMCC (China General Microbiological Culture Collection Center) No.26991 is screened out, the streptococcus zooepidemicus mutant strain is obtained through screening by adopting a phage secondary infection method, resistance experiments and genetic stability experiments prove that the screened strain is an anti-phage strain, the resistance can be stably inherited, and the strain can be used as a high-yield streptococcus zooepidemicus mutant strain. The strain not only has good resistance to bacteriophages, but also has excellent high-yield hyaluronic acid characteristics, and can be used as an industrial fermentation strain of hyaluronic acid.

The invention relates to the field of industrial fermentation and protein production. Specifically, the present invention relates to a method for producing a protein of interest in a fermentation medium, the method comprising the steps of: a) inoculating the fermentation medium with a Bacillus host cell comprising a gene encoding the protein of interest under the control of a promoter; b) culturing the Bacillus host cell in the fermentation medium under conditions that facilitate growth of the Bacillus host cell and expression of the protein of interest, c) adding sulfate to the fermentation medium to achieve a sulfate concentration of at least 20 mM in the fermentation medium; and d) precipitating and / or crystallizing the protein of interest during culture. Further contemplated is the use of a high sulfate concentration in the fermentation medium for the production of a protein of interest in a Bacillus host cell and a crystalline protein of interest obtained or obtainable by the method of the invention.

The application discloses a kind of genetic engineering escherichia coli plasmid expression culture medium and fermentation process, including basic medium and feed medium;The basic medium includes glycerol, yeast hydrolysate, yeast peptone, L-glutamic acid, L-valine, L-isoleucine, arginine, tyrosine, lysine, proline, glycine, ammonium sulfate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, citric acid, magnesium sulfate, ferrous sulfate, zinc sulfate, calcium chloride, manganese chloride, cobalt chloride, copper chloride, sodium ethylenediaminetetraacetate;Feed medium includes glycerol, citric acid, magnesium sulfate, ferrous sulfate, zinc sulfate, calcium chloride, manganese chloride, cobalt chloride, copper chloride, sodium ethylenediaminetetraacetate.The application inhibits the accumulation of harmful metabolites, and creates the optimal intracellular environment for stable replication of plasmid.In industrial fermentation scale, the amount of plasmid harvested per liter of fermentation broth is more than 1 gram, and the supercoiling ratio of plasmid DNA is stably maintained at more than 90%, with minimal batch-to-batch variation.

The invention provides a method and a kit for detecting the content of grease in yeast. The method for detecting the content of grease in yeast comprises the following steps: S1, preparing a sodium chloride solution with a series of concentration gradients; s2, sequentially adding bacterial liquid containing yeast to be detected into the sodium chloride solution, uniformly mixing, then carrying out centrifugal treatment, and after the centrifugal treatment, observing the initial suspended salinity, the middle suspended salinity and the complete suspended salinity of the yeast in the sodium chloride solution; s3, determining the grease content in the to-be-detected yeast according to the initial suspension salinity, the middle suspension salinity and the complete suspension salinity. According to the method for detecting the content of the grease in the yeast cells by using the saline water with different concentrations, the range of the content of the grease in the yeast cells, which can be detected, is about 10%-60%, the detection precision is about 10%, and the method has important significance on rapid detection of the content of the grease in industrial fermentation of the yeast.

This invention belongs to the field of microbial metabolic engineering and synthetic biology, specifically relating to a strain for high-yield... R,R -2,3-Butanediol ( R,R Salt-tolerant Jeju Bacillus DT01 IM3 DLB6 (-2,3-BDO), production strain constructed from this strain and its application in fermentation production. R,R Applications of -2,3-BDO. This invention screens a high-yielding strain through heavy ion beam irradiation mutagenesis. R,R Jeju Bacillus DT01 IM3 DL B6 with -2,3-BDO, preservation number CGMCC No. 37311. This strain exhibits excellent salt tolerance and low endotoxin properties. The production strain constructed from it has extremely high product synthesis capabilities, enabling high-concentration, high-intensity industrial fermentation production.

A strain for high-yield production of milbemycin and use thereof is disclosed. In the present invention, an original strain MI-W1-2017001 is subjected to mutation breeding by an atmospheric and room temperature plasma mutation method to obtain a high-yield strain HZMRYB-5. The fermentation level of the strain is improved by about 70% compared with the original strain, and the fermentation level is further improved by about 35% through optimization of a fermentation process and a formulation, so that the fermentation cost of the product is further reduced, and requirements for the industrial fermentation level are met. On the basis of optimizing the fermentation process, the ratio of A3 and A4 in a fermentation liquid is nearly 3:7 through mixed fermentation of the strain MI-W1-2017001 and the strain HZMRYB-5, so that market demands are greatly met, the process steps of separation, purification and then mixing are reduced.

The invention provides a sensor-based cordyceps militaris fermented beverage fermentation evaluation method and system, and relates to the technical field of fermentation engineering.Multi-frequency ultrasonic signals are transmitted and received through an ultrasonic transceiver array deployed on the outer wall of a fermentation tank; and reconstructing the signal into an acoustic parameter three-dimensional distribution image of the fermentation liquid in the fermentation tank by utilizing a tomography algorithm. And based on a pre-established mapping relation, mapping the acoustic parameter three-dimensional distribution image into three-dimensional distribution of the fermentation state parameters. And finally, based on the three-dimensional distribution of the fermentation state parameters, evaluating the overall real state of the internal environment of the fermentation tank to obtain spatial non-uniformity quantitative data of the internal environment of the fermentation tank, and generating an instruction for regulating and controlling the fermentation process according to the data. The method effectively solves the problem that monitoring of a traditional local sensor is not accurate due to non-uniformity of the internal environment of a large industrial fermentation tank in the prior art.

The application discloses a recombinant Escherichia coli for efficiently expressing dsRNA, which is a TG1 strain with gene defects and into which the following plasmids are introduced: a first plasmid comprising a T7 RNA polymerase expression module; and a second plasmid comprising a dsRNA expression module. rnc The application also discloses a method for producing dsRNA, and application of the recombinant Escherichia coli in preparing nucleic acid pesticides. rnc The application provides a new recombinant Escherichia coli with gene defects for producing dsRNA in the field of nucleic acid pesticides. rnc The knockout has no significant influence on the growth rate of the recombinant Escherichia coli, and the recombinant Escherichia coli has great industrial fermentation potential. The recombinant Escherichia coli adopts a double-plasmid expression system, and the dsRNA expression module structure with double T7 terminators is used to improve the expression amount and purity of dsRNA synthesis.

The invention provides a method for producing propionic acid and calcium salt thereof through fermentation, and belongs to the technical field of agricultural microbial fermentation. The method for producing propionic acid by fermentation comprises the following steps: (1) activating acid-producing propionibacterium with the preservation number of CGMCC1.2230, inoculating the acid-producing propionibacterium to a special seed culture medium for propionic acid, and standing and culturing for 48-72 hours under the anaerobic condition at the temperature of 37 + / -0.5 DEG C; and (2) inoculating the obtained bacterial liquid into a fermentation medium according to an inoculation ratio of 2.8-3.2%, fermenting under an anaerobic condition at 37 + / -0.5 DEG C and a stirring speed of 80-100rpm until the content of propionic acid is not obviously changed, and adjusting the pH value by using a calcium hydroxide suspension with a solid-to-liquid ratio of 15-20% during fermentation. According to the method, the yield of propionic acid produced by fermentation of the propionibacterium propionate is increased by adjusting the proportion of fermentation substrates and controlling the fermentation pH in a segmented mode, the yield of propionic acid produced by fermentation of the propionibacterium propionate can reach 16.23 g / L, and the production intensity is 0.225 gL <-1 > h <-1 >. The mixed fermentation ensures the realization of high-titer propionic acid production, and provides a theoretical basis for increasing the yield of propionic acid produced by industrial fermentation to a certain extent.

The invention belongs to the technical field of microorganisms, and particularly relates to a high-yield high-temperature-resistant pectinase strain and application thereof. The invention provides an aspergillus niger mutant strain AR-11 for producing high-yield and high-temperature-resistant alkaline pectinase, the enzyme activity level of pectinase produced by utilizing the strain for industrial fermentation can reach 8000-8200U / mL or more, the production cost is greatly reduced, and large-scale application, energy conservation and emission reduction of the alkaline pectinase are facilitated. The produced pectinase has high enzyme activity under a high temperature condition, keeps the stability of the enzyme activity in a wide pH value range, can meet the requirements of a pectinase preparation in the aspects of ramie degumming and textile refining, and can be widely applied to the ramie degumming industry.

The application discloses a Pediococcus pentosaceus with the functions of lowering cholesterol and inhibiting bacteria and a high-density industrial production fermentation medium of the Pediococcus pentosaceus, the Pediococcus pentosaceus is Pediococcus pentosaceus PG-2, and the preservation number is GDMCC No: 62392.The high-density industrial fermentation medium of the Pediococcus pentosaceus PG-2 contains 40 g / L of sugarcane molasses liquid, 20 g / L of corn syrup dry powder, 1.5 g / L of dipotassium hydrogen phosphate, 5 g / L of sodium acetate, 1 mL / L of Tween 80, 2 g / L of triammonium citrate, 0.2 g / L of magnesium sulfate heptahydrate, 0.05 g / L of manganese sulfate tetrahydrate, and the rest is water.The Pediococcus pentosaceus is cultured by using the medium, the bacterial concentration is significantly higher than that of MRS medium, a high-density bacterial body amount is effectively obtained, the utilization rate of industrial by-products is improved, the production cost is greatly reduced, and the production efficiency is improved.

This invention belongs to the field of bioengineering, specifically disclosing a 3-phosphoglycerate dehydrogenase mutant with improved enzyme activity and thermostability. Compared to the wild-type 3-phosphoglycerate dehydrogenase from *Corynebacterium glutamicum*, the mutant contains one mutation from among P85Q, D365Y, A389T, M196V, A183V, and I231V. Enzyme activity assays after induced expression and purification showed that the mutant's enzyme activity was 1.5–3.6 times higher than the wild type, and its thermostability was significantly improved. Therefore, the beneficial mutant provided by this invention lays a good foundation for the industrial fermentation production of L-serine and L-cysteine.

The application discloses a method for producing feed by using glutamic acid isoelectric mother liquor, and belongs to the technical field of industrial fermentation. The technical scheme of the application comprises the following steps: first, the glutamic acid isoelectric mother liquor is subjected to ceramic membrane and ultrafiltration membrane treatment to obtain membrane filtrate and concentrated solution; the concentrated solution is subjected to plate and frame filtration and drying to obtain bacterial protein; the obtained membrane filtrate is subjected to electrodialysis treatment to obtain concentrated water and dilute water; the concentrated water is subjected to evaporation crystallization and solid-liquid separation to obtain ammonium sulfate crystals; and the dilute water is subjected to concentration and drying to obtain feed protein, so that the method for producing feed from the glutamic acid isoelectric mother liquor is realized. The treatment method can increase the yield of feed, improve the yield of ammonium sulfate and bacterial protein, and has great economic and environmental benefits.

The invention relates to a fermentation medium and application thereof in industrial fermentation of animal bifidobacterium subsp. Lactis, the fermentation medium is composed of the following materials by weight: 2-5% of glucose; 1-5% of a yeast extract; 0.1 to 0.5 percent of sodium acetate; 0.05 to 0.2 percent of magnesium sulfate; and the balance of water. Under the condition that the yield and the activity of the bacterial powder are not influenced, the supply chain operation cost and the material purchasing cost are reduced by 20-30%, and meanwhile, the operation error of staff can be reduced; and a technical support is provided for cost reduction and benefit improvement of other probiotics in the industrial field.

The invention provides a method for producing Ectoine through salt impact-glutamine synergistic feeding, and relates to the field of fermentation engineering. The invention particularly relates to a metabolism regulation strategy of salt impact in cooperation with glutamine supplement. According to the method, after salt impact is applied in the logarithmic phase of halophilic bacteria, glutamine is accurately supplemented to activate a thallus stress synthesis pathway. Through the method, the yield of Ectoin can be remarkably increased, the fermentation period is shortened, and the metabolic activity of thalli is enhanced. The method is simple and convenient to operate and controllable in cost, and an innovative process solution is provided for efficient production of industrial fermentation of the halophilic bacteria.

The invention relates to the field of industrial fermentation and protein production. Specifically, the present invention relates to a method for producing a protein of interest in a fermentation medium, the method comprising the steps of: a) inoculating the fermentation medium with a bacillus host cell comprising a gene encoding the protein of interest under the control of a promoter; b) culturing the Bacillus host cell in the fermentation medium under conditions that facilitate growth of the Bacillus host cell and expression of the protein of interest, c) adding sulfate to the fermentation medium to achieve a concentration of sulfate of at least 20 mM in the fermentation medium; and d) precipitating and / or crystallizing the protein of interest during culture; wherein the fermentation medium comprises an amino acid derivative in an amount of from 0 g / l to 30 g / l of the fermentation medium. Further contemplated is the use of a combination of a sulfate and an amino acid derivative for the production of a protein of interest in a Bacillus host cell in a fermentation medium and a crystalline protein of interest obtained or obtainable by the method of the invention.

The application discloses a kind of recombination of Pseudomonas putida for producing L-lactic acid using whole-component lignocellulose hydrolysate and its construction method and application, belong to the field of metabolic engineering.The application uses Pseudomonas putida KT2440 as chassis, realizes carbon flow to pyruvic acid convergence and prevents product degradation by knocking out aceEF 、 ppsA And lldD Gene;By in-situ replacement, introduce optimized Lactobacillus plantarum ldhL1 Reconfiguration L-lactic acid synthesis pathway;By integration xylD-yjhH Tandem expression cassette enables xylose utilization;And by quorum sensing promoter P Rox306 Overexpression maeB Realize funnel conversion of lignin monomer.The application innovatively uses lignin derivatives in hydrolysate as natural donor of endogenous acetyl-coenzyme A, makes up lethal growth defect caused by knocking out aceEF The engineering bacteria of the application realize the synchronous high-efficiency conversion of glucose, xylose and aromatic monomer under the condition of not adding exogenous auxiliary carbon source and chemical inducer, which greatly reduces the industrial fermentation cost.

The invention discloses a method for biosynthesizing rebaudioside based on an in-vivo constructed UDP (User Datagram Protocol) recovery system, and belongs to the technical field of biology. A UDP recovery system is constructed in saccharomyces cerevisiae, specifically, the saccharomyces cerevisiae M24 is taken as a host, synthetic pathway coding genes including a sucrose synthase gene SUS3 and a polyphosphate dependent glucokinase gene PPGK are integrally expressed through a CRISPR Cas9 editing technology, and the genetically engineered bacterium is used for fermentation production of rebaudioside. The gene for endogenous regulation and control of UDP glucose synthesis is enhanced, a UDP recovery system is constructed at the same time, supply of UDP glucose is improved so as to improve the catalytic efficiency of enzyme, and therefore the yield of rebaudioside is increased. The breakthrough provides key technical support for industrial fermentation synthesis of rebaudioside, especially rebaudioside M, and has significant economic benefits and environmental protection values.

The invention discloses a glycosylation-free human enterokinase mutant as well as recombinant expression and application thereof, belongs to the field of genetic engineering and protein technical engineering, and solves the problems of disordered glycosylation modification, low enzymatic activity and low protein expression quantity during recombinant expression of enterokinase in an enterokinase microbiological cell factory. The method comprises the following steps: firstly, carrying out single-site mutation, double-site mutation, three-site mutation and four-site mutation on the basis of a human enterokinase wild type, and constructing a pichia pastoris cell factory to obtain a series of human enterokinase mutants without glycosylation modification. Through calculation design and rational transformation, partial or non-glycosylation is subjected to deep fixed-point transformation, and pichia pastoris cell factory expression is constructed, so that the expression quantity and the total enzyme activity of the mutant are greatly improved. The highest enzyme activity of the glycosylation-free enterokinase mutant generated by fed-batch fermentation in a pichia pastoris cell factory reaches 7.8 * 10 < 4 > U / L, and the enterokinase mutant has the capacity of large-scale industrial fermentation generation and application.

The present invention relates to the field of genetic engineering technology, and in particular to an engineered Escherichia coli strain that produces N-acetylneuraminic acid, as well as its construction method and application. Based on existing engineered bacteria, the present invention further optimizes the metabolic pathway from glucose to N-acetylmannosamine and knocks out the glmM gene to reduce the production of non-related metabolites. The resulting genetically engineered bacteria significantly enhance the Escherichia coli's ability to produce Neu5Ac in fermentation experiments, demonstrating promising prospects for industrial fermentation applications.

The invention relates to the technical field of construction of recombinant bacteria, in particular to recombinant corynebacterium glutamicum for producing L-ornithine as well as a construction method and application of the recombinant corynebacterium glutamicum for producing L-ornithine, corynebacterium glutamicum SO30 is taken as an original strain, a SigD gene and a FruR gene are sequentially knocked out through a suicide plasmid pK18mobsacB mediated homologous recombination technology, and double knockout recombinant corynebacterium glutamicum SO30-SigD-FruR is constructed. The L-ornithine yield of the strain subjected to shake-flask fermentation reaches 51.88 g / L and is increased by 56.7% compared with that of an original strain SO30; the fermentation yield of a 5L bioreactor reaches 123 g / L and is increased by 57.6% compared with that of an original strain SO30, and the cell biomass and the sugar conversion rate are remarkably increased. Through global transcriptional regulation factor transformation, the existing yield bottleneck is broken through, and efficient strains and technical support are provided for industrial fermentation production of L-ornithine.