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Bacillus subtilis and use thereof in bio-control preparation for preventing and controlling powdery mildew

A technology of Bacillus subtilis and biocontrol preparations, applied in the application, biocide, fungicide and other directions, to achieve the effects of not producing industrial three wastes, good curing effect, and easy industrial production

Inactive Publication Date: 2011-11-23
滨州国虹生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the control effects of biocontrol strains on powdery mildew come from the results of greenhouse experiments, and most of the strains are difficult to have a good control effect on powdery mildew due to the influence of ultraviolet rays or other environmental factors in the open field.

Method used

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  • Bacillus subtilis and use thereof in bio-control preparation for preventing and controlling powdery mildew
  • Bacillus subtilis and use thereof in bio-control preparation for preventing and controlling powdery mildew
  • Bacillus subtilis and use thereof in bio-control preparation for preventing and controlling powdery mildew

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Strain activation and propagation:

[0036] The strain was activated by streaking on LB solid medium (1.0% tryptone, 0.5% yeast extract, 1.0% sodium chloride, pH 7.0, 1.5% agar), and streaking it in LB liquid medium (1.0% tryptone , 0.5% yeast extract, 1.0% sodium chloride, pH value is about 7.0) propagation to obtain fermented seeds.

[0037] Industrial fermentation:

[0038] Medium: corn flour 1%, soybean flour 1.5%, yeast powder 0.1%, peptone 0.5%, potassium dihydrogen phosphate 0.8%, calcium carbonate 0.15%, magnesium sulfate 0.02%, antifoaming agent 0.005%, sucrose 2.0%, pH 7.0; Fermentation conditions: Fermentation temperature is 28-35℃, aeration rate is 0.4v / v / min, stirring speed is 100 revolutions / min, 24 hours; during the fermentation process, sampling once every 2 hours to determine pH , Observe the colony morphology. When the number of spores reaches 5%, add the material once. When the number of spores reaches 90%, stop the fermentation, and observe whether it is...

Embodiment 2

[0040] Strain activation and propagation:

[0041] The strain was streaked and activated on LB solid medium (1.0% tryptone, 0.5% yeast extract, 1.0% sodium chloride, pH value around 7.0, 1.5% agar), and in LB liquid medium (1.0% tryptone , 0.5% yeast extract, 1.0% sodium chloride, pH value is about 7.0) propagation to obtain fermented seeds.

[0042] Inoculate fermented seeds to 0.8% corn flour, 1.5% soybean flour, 0.1% yeast powder, 0.5% peptone, 0.8% potassium dihydrogen phosphate, 0.15% calcium carbonate, 0.02% magnesium sulfate, 0.005% defoamer, pH 7.0; Fermentation conditions: fermentation temperature is 28℃, aeration rate is 0.2v / v / min, stirring speed is 100 revolutions / min, fermentation time is 36 hours; during the fermentation process, take samples once every 2 hours, measure pH value, and observe Colony morphology. When the number of spores reaches 5%, add the material once. When the number of spores reaches 90%, stop the fermentation, and observe whether it is a biologi...

Embodiment 3

[0044] Strain activation and propagation:

[0045] The strain was streaked and activated on LB solid medium (1.0% tryptone, 0.5% yeast extract, 1.0% sodium chloride, pH value around 7.0, 1.5% agar), and in LB liquid medium (1.0% tryptone , 0.5% yeast extract, 1.0% sodium chloride, pH value is about 7.0) propagation to obtain fermented seeds.

[0046] Inoculate fermented seeds to 0.8% corn flour, 1.5% soybean flour, 0.1% yeast powder, 0.5% peptone, 0.8% potassium dihydrogen phosphate, 0.15% calcium carbonate, 0.01% magnesium sulfate, 0.005% defoamer, pH 5.5; Fermentation conditions: fermentation temperature is 30℃, aeration rate is 0.2v / v / min, stirring speed is 100 rpm, 34 hours; during the fermentation process, samples are taken every 2 hours, pH value is measured, and colony morphology is observed . When the number of spores reaches 5%, add the material once. When the number of spores reaches 90%, stop the fermentation, and observe whether it is a biological agent through micros...

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Abstract

The invention discloses bacillus subtilis and use thereof in bio-control preparation for preventing and controlling powdery mildew and belongs to the field of microbial pesticides. The strain is (bacillus subtilis)FBR1 with a collection number of CGMCC No.4915. The industrial fermentation culture medium for the bacillus subtilis is prepared from 0.8 percent of corn flour, 1.5 percent of soybean flour, 0.1 percent of yeast powder, 0.5 percent of peptone, 0.8 percent of monopotassium phosphate, 0.15 percent of calcium carbonate, 0.02 percent of magnesium sulfate and 0.001 percent of defoamer, and by the steps of adjusting the pH value to 7.0, fixing volume by using water, and fermenting for 24 to 36 hours under conditions of a fermentation temperature of 28 to 35 DEG C, a ventilation volumeof 0.2 to 0.4 v / v / min and a stirring speed of 100 to 150r / min. The invention also discloses a bio-control preparation which contains bacillus subtilis and can be used for preventing and controlling powdery mildew in economic crops such as melons, vegetables, tobacco and strawberry in place of spray chemical medicines without polluting the environment.

Description

Technical field [0001] The invention belongs to the field of microbial pesticides, and mainly relates to a Bacillus subtilis strain and its industrial preparation method. The invention also relates to a biocontrol agent for preventing powdery mildew of cash crops such as melons, tobacco, vegetables, and strawberries. Background technique [0002] Powdery mildew has many species and wide distribution. There are currently about 650 kinds of officially named in the world. Fungi belonging to 3 genera and 6 species can cause powdery mildew in melons. Melon powdery mildew is mainly caused by the powdery mildew fungus (Podosphaera xanthii, formerly known as Sphaerotheca fuliginea) and powdery mildew (Golovinomyces cichoracearum, formerly known as Erysiphe cichoracearum). It is a worldwide disease that is common under various cultivation conditions. It can happen, perennially harmful, and seriously hinder the green production of melons. Powdery mildew has many physiological races, rapi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/125
Inventor 范成明熊国如
Owner 滨州国虹生物科技有限公司
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