17 results about "Colony morphology" patented technology

The invention discloses a bacterial colony image time sequence classification tracking method based on dynamic time warping and label propagation, and solves the problems of inconsistent classification and accurate segmentation of bacterial colonies with different sizes caused by the change of bacterial colony morphology along with time. The method comprises the following steps: acquiring a bacterial colony image feature sequence and constructing a bacterial colony feature distance matrix; calculating an accumulated distance matrix based on a dynamic time warping algorithm, and calculating accumulated distance values point by point through a recursion formula; through a path backtracking algorithm, finding a time point matching path which minimizes an accumulated distance value between bacterial colonies, and obtaining an optimal time alignment relationship between feature sequences of different bacterial colonies; constructing a similarity matrix, and calculating a similarity value between bacterial colonies; and a classification corresponding relation between time points is established by adopting a cross-time-point target association algorithm, and the bacterial colony classification label at the first time point is propagated to the subsequent time point, so that the consistency of the time sequence classification labels is ensured.

The application belongs to the technical field of salt-tolerant growth-promoting bacteria, and particularly relates to a strain of Dai's bacteria A17, a bacterial agent, a method and application. Dyella marensis The application provides a strain of Dai's bacteria (A17) with a preservation number of CGMCC No.34686. The strain of Dai's bacteria A17 is gram-negative, and the bacterial body morphology is rod-shaped. The colony morphology is circular, the colony diameter is small, the surface is smooth, wet, shiny, slightly yellow, and the edge is complete. The strain of Dai's bacteria A17 can simultaneously significantly improve the contents of tanshinone and cryptotanshinone in the hairy roots of Salvia miltiorrhiza.

The invention discloses a complex microbial inoculant and application thereof, a potato residue bio-organic fertilizer and a preparation method and application thereof, belongs to the technical field of microorganisms, and solves the problems of high difficulty in resource utilization of potato residues and poor fertilizer efficiency of a traditional microbial preparation. The complex microbial inoculant is prepared by mixing a P.megatherium bacterial liquid and a Paenibacillus tuzhoensis bacterial liquid according to a mass ratio of 1: 1. The separation method comprises the following steps: selecting green peels, sprouting tubers and buds from disinfected potatoes, grinding, adding sterile water, coating on an LB culture medium plate, culturing in an incubator at 28-32 DEG C, selecting representative single colonies by a plate streaking method according to the colonial morphology, and repeatedly purifying to finally obtain the strain. The preparation method of the potato residue bio-organic fertilizer comprises the following steps: uniformly mixing the organic fertilizer, the potato residues, the biochar and the soil; inoculating a complex microbial inoculant; and fermenting and culturing. The two strains have a synergistic effect, so that the fertility of the potato residue bio-organic fertilizer can be obviously improved.

The invention relates to the technical field of biological genes, in particular to a gene directed editing system applied to mycoplasma bovis, which is an editing system for expressing a combination of a single plasmid of GP35 single-chain annealing protein and a targeting fragment ssDNA (single-stranded deoxyribonucleic acid), and comprises an expression plasmid of a GP35 gene and the ssDNA of the targeting fragment. The invention relates to a gene directed editing method applied to mycoplasma bovis. The gene directed editing method comprises the following steps: constructing a mycoplasma bovis strain for expressing GP35 single-chain annealed protein, constructing an ssDNA single chain of a targeting fragment, and performing gene editing on a target gene to obtain a pdhC gene deleted strain. According to the invention, a KanR gene is successfully inserted at the 158bp position of a pdhC gene coding region of a mycoplasma bovis PG45 strain, so that a mycoplasma bovis pdhC gene oriented deletion strain is obtained. According to the invention, the metabolism-related gene directed deletion strain M. bovis PG45 [delta] pdhC is successfully constructed, and the pdhC protein is characterized to have the ability of regulating the growth rate of mycoplasma bovis and maintaining colonial morphology.

The invention discloses a method suitable for manufacturing a microbial specimen by sealing UV glue, and belongs to the technical field of microbial specimen manufacturing, microorganisms are inoculated to a plate culture medium for culture, then a cultured microbial plate is sterilized and dried, the preheated UV glue is poured into the dried microbial plate, and the microbial specimen is obtained. And carrying out auxiliary defoaming by using a hot air gun or a syringe needle or a vacuum pump, and finally, irradiating for 5 minutes or 8 minutes by using a 36W ultraviolet lamp, so as to obtain the microbial specimen. The method is mainly used for keeping morphological characteristics such as bacterial colony shape and color of microorganisms to the greatest extent after a series of specimen manufacturing process steps are carried out on a cultured microorganism plate with relatively obvious bacterial colony morphological characteristics, the processing steps are simple, convenient and safe, the prepared specimen is stable, and the method is suitable for large-scale popularization and application. The exhibition period is longer, and the exhibition effect is better.

The invention discloses a bacillus subtillis colony morphology identification and counting method based on deep learning, and relates to the technical field of microbial colonies, the method comprises the following steps: obtaining a colony image of to-be-identified bacillus subtillis; inputting the bacterial colony image into a trained bacterial colony detection model; outputting a form recognition result of the bacterial colony image by the bacterial colony detection model; counting bounding boxes of all bacterial colonies in the form recognition result to obtain a bacterial colony counting result and outputting the bacterial colony counting result; the bacterial colony detection model is based on a deep learning target detection model and is obtained by training a data set of bacillus subtillis bacterial colony images of various morphological categories. According to the method, the bacterial colony detection is realized based on the trained model through the image data set specially constructed by the polymorphic features of the bacillus subtillis, so that the technical problems that a traditional bacterial colony recognition technology depends on manpower and is low in efficiency are solved, accurate and automatic recognition and counting of bacterial colony morphology are realized, and the detection efficiency is improved. The efficiency and the reliability of microbial phenotype analysis are improved.

This invention provides a method for evaluating the permeability of the paste-like region of titanium alloys based on β-spot morphology, enabling accurate quantitative assessment of the permeability of the paste-like region of titanium alloys. It belongs to the field of titanium alloy material technology and includes: obtaining a sample of the titanium alloy and acquiring a visual image of β-spots in a representative region of the sample; identifying crystal regions in the acquired β-spot visual image, identifying columnar crystal regions, equiaxed crystal regions, and crystal regions where columnar crystals transform to equiaxed crystals; dividing the permeability analysis area into n partitions along the analysis direction; calculating the morphological parameters of the β-spots in each partition, including area fraction, average diameter, and fractal dimension; and calculating the liquid phase permeability of the paste-like region in each partition based on the extracted morphological parameters of the β-spots. This invention provides key quantitative parameters for a deeper understanding of the β-spot formation mechanism and macroscopic segregation process.

The application discloses a method for screening coenzyme Q10 high-yield strains based on colony morphology, and belongs to the technical field of microbial fermentation and strain breeding, and comprises the following steps: diluting and coating a starting bacterial liquid on a flat plate culture medium, and directly performing preliminary screening according to the morphological characteristics of single colonies after static culture, wherein the single colony with the following characteristics is selected as a target strain: the color is dark green, the morphology is circular, the edge is complete, and the diameter is 8-12 mm; then, the target strain is sequentially subjected to slant expansion culture, seed liquid culture and fermentation culture; finally, the coenzyme Q10 high-yield strain is verified and obtained by measuring the bacterial concentration of the fermentation liquid and the coenzyme Q10 yield.

The invention discloses a method for measuring the total number of plate bacterial colonies and application of the method. The determination method comprises the following steps: preparing a plate counting culture medium, and carrying out water bath heat preservation on the plate counting culture medium; the plate counting culture medium contains peptone, yeast extract powder, glucose, agar, sodium pyruvate, L-cysteine hydrochloride, magnesium sulfate and hemin; performing gradient dilution on the target bacteria; fully and uniformly mixing the sample homogenate with a plate counting culture medium; covering the surface of the solidified plate counting culture medium with a layer of same plate counting culture medium to form a covering layer; carrying out constant-temperature culture on the solidified flat plates with various dilution degrees; when macroscopic bacterial colonies are formed in the culture medium plate, selecting a plate with 30-300 bacterial colonies on each plate for counting. The method does not need coating operation, can effectively reduce the pollution risk, shortens the detection time, has clear colonial morphology and reliable data, and is suitable for rapid determination of the total number of colonies in various samples.

The screening method comprises the following steps: S1, sample treatment: taking a high-temperature yeast sample, adding sterile water, oscillating and uniformly mixing, and then carrying out gradient dilution; s2, primary screening: coating a PDA culture medium with the diluent, and separating and purifying yeast strains through colonial morphology observation and microscopic morphology observation after constant-temperature culture; s3, secondary screening: inoculating the purified yeast strain to a tributyrin solid culture medium, performing constant-temperature culture, and screening out a strain with relatively high ester production capacity according to the size of a transparent circle around a bacterial colony; s4, molecular biological identification: constructing a clear strain taxonomic status through sequence alignment and phylogenetic tree construction; and S5, fermentation condition optimization: determining key influence factors through a single factor test, and optimizing fermentation conditions by adopting a response surface test to obtain optimal ester production fermentation parameters. According to the method, high-efficiency screening and fermentation condition optimization of the high-yield ester yeast in the high-temperature yeast for making hard liquor are realized through targeted screening and accurate identification.

The screening method comprises the following steps: S1, separation and purification of mould: stacking fermented grains of Maotai-flavor liquor, diluting the fermented grains by using sterile normal saline in a gradient manner, inoculating the fermented grains to a PDA culture medium by using a dilution coating plate method, culturing at constant temperature, and purifying by using a plate streaking method to obtain pure mould strains; s2, screening high-temperature-resistant strains: sequentially culturing the pure strains obtained in the step S1 under a temperature gradient, and screening out high-temperature-resistant mould strains with the highest tolerable temperature being greater than or equal to 55 DEG C; and S3, identifying the strain: observing a bacterial colony and a microstructure through morphology, and determining the species of the high-temperature-resistant strain by combining ITS gene PCR amplification, sequencing and phylogenetic tree construction.

This invention discloses a method for systematically isolating and identifying Verticillium wilt of cotton, with the following steps: (1) disease sample collection and initial treatment; (2) strain culture: transfer the diseased tissue to a culture dish containing ampicillin PDA medium and culture it in a constant temperature incubator at 28°C for 3-5 days; (3) streak isolation and colony screening: place the culture dish under a microscope to directly observe the colony morphology. If the colony has the characteristic morphological features of Verticillium wilt of cotton, then the culture dish is confirmed to contain Verticillium wilt of cotton colonies; (4) single colony purification: from the culture dish containing Verticillium wilt of cotton colonies confirmed in step (3), pick a single colony with a sterile inoculation loop, transfer it to a new PDA medium for streak culture, and perform 2-3 consecutive passages for purification to obtain a purified strain; (5) DNA extraction and molecular identification; (6) pathogenicity verification inoculation; (7) re-isolation verification.

The invention belongs to the technical field of salt-tolerant growth-promoting bacteria, and particularly relates to a Delhi A17 strain, a microbial agent, a method and application. The invention provides a strain of Delta marensis A17, and the preservation number of the Delta marensis A17 is CGMCC (China General Microbiological Culture Collection Center) No.34686. The Gram staining of the Delhi bacteria A17 is negative, and the thallus is in a rod shape; the colonial morphology is round, the diameter of the colonial is small, the surface is smooth, wet, glossy and yellowish, and the edge is complete. The Delhi A17 disclosed by the invention can be used for remarkably improving the content of miltirone and cryptotanshinone in hairy roots of salvia miltiorrhiza bunge at the same time.

The invention relates to a zpap gene and application thereof in regulation and control of the form of bacillus subtillis, and aims at the technical problem that a key genetic target for mediating opaque and semitransparent colony form conversion of the bacillus subtillis lacks in the prior art, the zpap gene (SEQ ID NO.1) is cloned, and a recombinant expression vector of the zpap gene is constructed and converted into the bacillus subtillis. The key technical means is to specifically induce the colonial morphology to be converted from opaque to semitransparent by up-regulating the expression of the zpap gene, and confirm that the process is related to the regulation of the integrity of the cell wall. According to the invention, the blank of a cell wall active genetic regulation mechanism is filled, and a new key target and an effective strategy are provided for performance improvement of industrial strains.

This invention relates to the field of image data processing technology, and discloses a multimodal image acquisition and processing method for automated counting of microbial colonies in a production line. The method includes the following steps: imaging a culture dish under test using a multimodal physical imaging module to acquire three-modal fused image data containing at least polarization characteristics, multispectral characteristics, and photometric stereoscopic characteristics; establishing a physical-neural hybrid reflection decoupling network and inputting the three-modal fused image data into the network; based on the diffuse reflection dominant image and the specular reflection dominant image, using a multi-scale colony generation and repair network to perform texture repair on reflective residue areas, wherein the repair network introduces a colony morphology prior constraint layer in the decoder section; outputting the repaired image and performing colony counting based on the repaired image. This invention possesses robustness in suppressing and repairing surface reflective interference and has strong generalization ability.

The invention provides a full-automatic bacterial colony counting system and counting method based on a secondary watershed, and belongs to the technical field of microbiological detection and analysis. Comprising a color segmentation module used for converting an input RGB image to an HSV space and separating bacterial colonies with different color features to obtain bacterial colonies with different color features; the adhered bacterial colony separation module is used for separating adhered bacterial colonies from the bacterial colonies with different color characteristics by using a secondary watershed algorithm to obtain separated bacterial colonies; and the morphological characteristic quantitative evaluation module is used for comparing the shape of the separated bacterial colony with a preset standard, removing impurities, and analyzing and evaluating the growth state of the bacterial colony to obtain the number of bacterial colonies meeting the preset standard. According to the method, the color distinguishing capability can be enhanced by utilizing the clustering characteristic of the HSV hue channel; a secondary watershed algorithm is adopted to improve the segmentation precision of the adhered bacterial colonies; bacterial colonies are screened through roundness parameters, and impurity interference is eliminated.