30 results about "Clostridium welchii" patented technology

The invention relates to a feed for reducing immature-rabbit diarrhea diseases and a feeding method of immature rabbit thereof. Additives of the feed comprise the following ingredients in parts by mass: 23 parts of honeysuckle flower extract, 23 parts of eucommia bark extract, 16 parts of feed small-peptides of, 12 parts of feed yeast and 12 parts of feed lactobacillus. The feeding method of immature rabbit comprises the following steps: selecting weaned immature rabbits which are 33-37 days old, and feeding the selected immature rabbits until the immature rabbits are 70 days old; respectively injecting rabbit haemorrhagic disease virus and rabbit Pakistan virus bigeminal-vaccines to the immature rabbits when the immature rabbits are 33 days old and 60 days old, and injecting clostridium welchii vaccines to the immature rabbits when the immature rabbits are 42 days old; fasting-weighing the immature rabbits in the morning during the feeding processes; adding 0.1-0.5% of the feed additive capable of reducing immature-rabbit diarrhea diseases into dietary of the immature rabbits, and uniformly stirring the feed additive and the dietary; utilizing a feeding mode of free food and water intakes; carrying out cleaning at eight o'clock in the morning, starting feeding at nine o'clock in the morning, weighing the immature rabbits again in the evening, and calculating feed consumptions; observing and recording the amount of the immature rabbits with diarrheas and the amount of the dead immature rabbits; fasting-weighing the rabbits when the 70 days of feeding is over; carrying out statistics of daily weight-gains and feed consumptions during the feeding periods; and analyzing feed conversion ratios and diarrhea rates. The feeding method is capable of improving production performances of the immature rabbits, reducing diarrhea rates and increasing economic benefits.

A disease germ type of colloid Se test paper for testing Clostridium welchii disease consists of plastic base plate, Se labeled pad formed by glass cellulose film with detection region and control region, sampling-adding film, water absorption paper and reaction film formed by nitric acid cellulose film with detection region and control region.

The invention discloses a clostridium welchii disease resistant transgenic plant vaccine and a preparation method thereof. At present, the protective ratio on immunization of clostridium welchii disease resistant vaccines is lower. The preparation method of the clostridium welchii disease resistant transgenic plant vaccine comprises the steps of: (1) with a plasmid or disease sample containing coded clostridium welchii toxin as a template, carrying out PCR (Polymerase Chain Reaction) or RT-PCR (Reverse Transcription-Polymerase Chain Reaction) amplification to obtain a clostridium welchii toxin gene, carrying out enzyme cutting on the obtained clostridium welchii toxin gene or the Ti or Ri plasmid respectively, recycling a target gene and a plant expression vector segment, connecting with and converting escherichia coli, carrying out enzyme cutting, PCR and sequencing identification on the recombinant Ti or Ri plasmid, converting agrobacterium tume faciens or agrobacterium rhizogene with the recombinant plasmid, and screening and identifying positive recombinant agrobacterium; and (2) carrying out vernalization, germination and splicing on plant seeds to obtain explants, infecting the explants respectively with the recombinant agrobacterium in the step (1), carrying out screening, subculturing, rooting and transplanting on the infected explants to obtain transformed plants, and screening and identifying positive transgenic plants.

The invention discloses anti-bacterial peptide PD22. The amino acid sequence of the anti-bacterial peptide PD22 is shown in SEQ ID NO:1. The anti-bacterial peptide PD22 has the advantages of being small in molecular weight, capable of being conveniently and artificially synthesized, low in cost, strong in killing effect on escherichia coli, salmonella, clostridium welchii and other bacteria, low in clostridium welchii and capable of remarkably decreasing the diarrhea rate of weaned pigs and increasing the growth speed of weaned pigs after being applied to weaned pig feed.

The invention relates to the field of animal bacteriology and provides a preparation method of an inactivated vaccine of A-type clostridium perfringens for clostridium welchii disease of a domestic rabbit. The preparation method comprises the following steps: adopting the A-type clostridium perfringens as an original strain, finally obtaining a bacterium solution by primary and secondary culture, mixing the bacterium solution and white oil adjuvant and preparing the inactivated vaccine of the A-type clostridium perfringens. The vaccine prepared by the method is immunized for the domestic rabbit, the protective rate reaches 100% after counteracting toxic substances, so that good immunoprotection action is achieved.

The invention belongs to the field of molecular biological detection. Specifically, the invention relates to detection of bovine diarrhea pathogens. More specifically, the invention relates to a composition for detecting six bovine diarrhea pathogenic pathogens, namely salmonella, clostridium welchii, escherichia coli K99, shigella dysenteriae, campylobacter jejuni and proteus mirabilis. Meanwhile, the invention further provides a kit containing the composition, application of the composition and a method for detecting and typing bovine diarrhea pathogens. The composition is mainly detected in one tube by using multiple fluorescent PCR and melting curve analysis methods, so that different pathogens can be treated differently, and the treatment and prevention are more effective.

The invention discloses a traditional Chinese medical composition for prevention and treatment of A type clostridiosis welchii, which consists of the following components by weight: 10-80 parts of mahonia fortunei, 5-50 parts of pulsatilla chinensis, 3-50 parts of yam, 3-30 parts of semen plantaginis, 3-30 parts of sanguisorba officinalis, 3-30 parts of rheum officinale and 5-45 parts of acalypha australis. The invention also discloses an extract prepared with the composition, a preparation method of the extract, a preparation prepared with the extract and application of the preparation. The composition, the extract and the preparation have broad values in medical application for prevention and treatment of the A type clostridiosis welchii.

The invention belongs to the technical field of agricultural microorganism application, and in particular relates to the screening and application of a probiotic bacillus licheniformis with high production of complex enzymes. The bacillus licheniformis of the invention has obvious antibacterial effect on common livestock and poultry enteropathogenic bacteria such as escherichia coli, staphylococcus aureus and Clostridium welchii. The invention includes the isolation and identification of the bacillus licheniformis strain, the research on biological characteristics and its application as a feed additive for livestock and poultry, and the like. The bacterial strain of the present invention produces cellulase, amylase and protease, is resistant to low pH value of gastric acid and high osmotic pressure of intestinal bile salt, has fast growth rate, strong stress resistance, strong antibacterial property and strong enzyme production ability , can be used to prepare microbial additives for livestock and poultry feed.

The invention discloses a Clostridium welchii phage, its phage composition and its application. The phage is Clostridium welchii phage PMQ06, which was preserved in the General Microbiology Center of China Committee for Microbial Culture Collection on May 15, 2020. Its deposit number is CGMCC No.19977. The phage has high-efficiency bactericidal activity, wide cleavage spectrum, and high temperature stability, and can be used as an active ingredient in the preparation of medicines, environmental disinfectants, feed additives, and bacteriostatic agents for preventing and treating Clostridioides welchii disease, etc., and can be widely used Applied to various links in the breeding process and product processing and production process, it can quickly, efficiently and safely kill pathogenic Clostridium welchii and even drug-resistant Clostridium welchii, effectively reduce the mortality of diseased chickens, and also has a safe, non-toxic Advantages of residual and no side effects.

The invention discloses an antimicrobial peptide PD22 and its application. The amino acid sequence of the antimicrobial peptide PD22 is shown in SEQ ID NO:1. The antimicrobial peptide PD22 in the present invention has small molecular weight, convenient artificial synthesis and low cost, strong bactericidal effect on bacteria such as Escherichia coli, Salmonella and Clostridium welchii, and low cytotoxicity, and can significantly reduce diarrhea in weaned piglets when used in feed for weaned piglets The advantages of high rate and increased growth rate of weaned piglets.

A disease germ type of colloid Se test paper for testing Clostridium welchii disease consists of plastic base plate, Se labeled pad formed by glass cellulose film with detection region and control region, sampling-adding film, water absorption paper and reaction film formed by nitric acid cellulose film with detection region and control region.

The invention discloses a clostridium welchii disease resistant transgenic plant vaccine and a preparation method thereof. At present, the protective ratio on immunization of clostridium welchii disease resistant vaccines is lower. The preparation method of the clostridium welchii disease resistant transgenic plant vaccine comprises the steps of: (1) with a plasmid or disease sample containing coded clostridium welchii toxin as a template, carrying out PCR (Polymerase Chain Reaction) or RT-PCR (Reverse Transcription-Polymerase Chain Reaction) amplification to obtain a clostridium welchii toxin gene, carrying out enzyme cutting on the obtained clostridium welchii toxin gene or the Ti or Ri plasmid respectively, recycling a target gene and a plant expression vector segment, connecting with and converting escherichia coli, carrying out enzyme cutting, PCR and sequencing identification on the recombinant Ti or Ri plasmid, converting agrobacterium tume faciens or agrobacterium rhizogene with the recombinant plasmid, and screening and identifying positive recombinant agrobacterium; and (2) carrying out vernalization, germination and splicing on plant seeds to obtain explants, infecting the explants respectively with the recombinant agrobacterium in the step (1), carrying out screening, subculturing, rooting and transplanting on the infected explants to obtain transformed plants, and screening and identifying positive transgenic plants.