30 results about "Clostridium welchii" patented technology

The invention relates to the field of animal bacteriology and provides a preparation method of an inactivated vaccine of A-type clostridium perfringens for clostridium welchii disease of a domestic rabbit. The preparation method comprises the following steps: adopting the A-type clostridium perfringens as an original strain, finally obtaining a bacterium solution by primary and secondary culture, mixing the bacterium solution and white oil adjuvant and preparing the inactivated vaccine of the A-type clostridium perfringens. The vaccine prepared by the method is immunized for the domestic rabbit, the protective rate reaches 100% after counteracting toxic substances, so that good immunoprotection action is achieved.

The invention discloses a novel strain Clostridium butyricum UCN-12 and a composition and application thereof. The preservation number of the novel strain in CGMCC (China General Microbiological Culture Collection Center) is CGMCC No.9652. As effective constituents fed to livestock, a fermentation culture of the strain, fermentation liquor or thalli obtained by centrifugating the fermentation culture and a dried product of the fermentation liquor or thalli have the effect of treating and / or preventing livestock clostridium welchii disease; no antibiotic is needed to be added in the whole process so that environment contamination caused by antibiotic residues is avoided; cholesterol and fat in eggs can be remarkably reduced, and eggy smell can be removed to improve the taste of eggs; the production performance of livestock is improved; and antenatal and puerperal constipation of sows can be treated and / or prevented to reduce smelly excrement of livestock and realize environment-friendly cultivation.

The invention relates to a feed for reducing immature-rabbit diarrhea diseases and a feeding method of immature rabbit thereof. Additives of the feed comprise the following ingredients in parts by mass: 23 parts of honeysuckle flower extract, 23 parts of eucommia bark extract, 16 parts of feed small-peptides of, 12 parts of feed yeast and 12 parts of feed lactobacillus. The feeding method of immature rabbit comprises the following steps: selecting weaned immature rabbits which are 33-37 days old, and feeding the selected immature rabbits until the immature rabbits are 70 days old; respectively injecting rabbit haemorrhagic disease virus and rabbit Pakistan virus bigeminal-vaccines to the immature rabbits when the immature rabbits are 33 days old and 60 days old, and injecting clostridium welchii vaccines to the immature rabbits when the immature rabbits are 42 days old; fasting-weighing the immature rabbits in the morning during the feeding processes; adding 0.1-0.5% of the feed additive capable of reducing immature-rabbit diarrhea diseases into dietary of the immature rabbits, and uniformly stirring the feed additive and the dietary; utilizing a feeding mode of free food and water intakes; carrying out cleaning at eight o'clock in the morning, starting feeding at nine o'clock in the morning, weighing the immature rabbits again in the evening, and calculating feed consumptions; observing and recording the amount of the immature rabbits with diarrheas and the amount of the dead immature rabbits; fasting-weighing the rabbits when the 70 days of feeding is over; carrying out statistics of daily weight-gains and feed consumptions during the feeding periods; and analyzing feed conversion ratios and diarrhea rates. The feeding method is capable of improving production performances of the immature rabbits, reducing diarrhea rates and increasing economic benefits.

The invention belongs to the technical field of agricultural microorganism application, in particular to the screening and application of a probiotic bacillus licheniformis of a high-yield compound enzyme. The bacillus licheniformis has obvious bacteriostatic effect on common livestock intestinal pathogenic bacteria such as escherichia coli, staphylococcus aureus and clostridium welchii. The screening and application of the probiotic bacillus licheniformis of the high-yield compound enzyme comprises the isolation and identification of bacillus licheniformis strains, the study of biological characteristics and the application as livestock feed additives. According to the screening and application of the probiotic bacillus licheniformis of high-yield compound enzyme, the strain of the invention produces cellulase, amylase and proteinase, is resistant to low pH value of gastric acid and high osmotic pressure of intestinal bile salt, has the characteristics of fast growth, high stress resistance, high bacteriostasis and high enzyme production ability, and can be used for preparing livestock feed microbial additives.

A disease germ type of colloid Se test paper for testing Clostridium welchii disease consists of plastic base plate, Se labeled pad formed by glass cellulose film with detection region and control region, sampling-adding film, water absorption paper and reaction film formed by nitric acid cellulose film with detection region and control region.

The invention discloses an antibacterial peptide PD22 and application thereof. The amino acid sequence of the antibacterial peptide PD22 is shown as SEQ ID NO: 1. The antibacterial peptide PD22 has the advantages that the molecular mass is small, the artificial synthesis is convenient, the cost is low, the functions of killing such bacteria as colibacillus, salmonella and clostridium welchii are strong and the cytoxicity is low; when the antibacterial peptide PD22 is applied to feed for weaned pigs, the diarrhea rate of the weaned pigs can be remarkably reduced, and the growth rate of the weaned pigs can be improved.

The invention discloses a clostridium welchii bacteriophage as well as a bacteriophage composition and application thereof, the bacteriophage is a clostridium welchii bacteriophage PMQ06 and is preserved in China General Microbiological Culture Collection Center on May 15, 2020, and the preservation number is CGMCC No.19977. The bacteriophage has the advantages of high-efficiency bactericidal activity, wide lysis spectrum and high temperature stability, can be used as an active ingredient for preparing drugs for preventing and treating chicken clostridium welchii disease, environmental disinfectants, feed additives, bacteriostats and the like, can be widely applied to various links in the breeding process and the product processing and production process, and has broad market prospects. The pathogenicity clostridium welchii and even the drug-resistant clostridium welchii are rapidly, efficiently and safely killed, the mortality rate of morbidity chickens is effectively reduced, and thefeed additive has the advantages of being safe, free of residues and free of side effects.

The invention discloses a clostridium welchii disease resistant transgenic plant vaccine and a preparation method thereof. At present, the protective ratio on immunization of clostridium welchii disease resistant vaccines is lower. The preparation method of the clostridium welchii disease resistant transgenic plant vaccine comprises the steps of: (1) with a plasmid or disease sample containing coded clostridium welchii toxin as a template, carrying out PCR (Polymerase Chain Reaction) or RT-PCR (Reverse Transcription-Polymerase Chain Reaction) amplification to obtain a clostridium welchii toxin gene, carrying out enzyme cutting on the obtained clostridium welchii toxin gene or the Ti or Ri plasmid respectively, recycling a target gene and a plant expression vector segment, connecting with and converting escherichia coli, carrying out enzyme cutting, PCR and sequencing identification on the recombinant Ti or Ri plasmid, converting agrobacterium tume faciens or agrobacterium rhizogene with the recombinant plasmid, and screening and identifying positive recombinant agrobacterium; and (2) carrying out vernalization, germination and splicing on plant seeds to obtain explants, infecting the explants respectively with the recombinant agrobacterium in the step (1), carrying out screening, subculturing, rooting and transplanting on the infected explants to obtain transformed plants, and screening and identifying positive transgenic plants.

The invention discloses a polymerase chain reaction (PCR) kit for detecting sheep clostridium perfringens. The kit comprises 250mu L of PCR premix buffer solution, 150mu L of ultrapure water, 50mu L of marker DL2000, 40mu L of 10mu mol / L upper primer, 40mu L of 10mu mol / L lower primer, 20mu L of negative control and 20mu L of positive control. By optimizing PCR conditions and replacing labor-consuming and time-consuming biochemical experiment and serological monitoring with the conventional PCR method, the PCR kit for detecting sheep clostridium perfringens disease is developed; and the kit can detect the sheep clostridium perfringens disease, can be used for the early and quick diagnosis of the sheep clostridium perfringens disease, has great significance for preventing and treating the sheep clostridium perfringens disease, can avoid errors caused by artificial judgment, shortens the detection time of the sheep clostridium perfringens disease, and has the characteristics of high speed, sensitivity and specificity and the like.

The invention discloses anti-bacterial peptide PD22. The amino acid sequence of the anti-bacterial peptide PD22 is shown in SEQ ID NO:1. The anti-bacterial peptide PD22 has the advantages of being small in molecular weight, capable of being conveniently and artificially synthesized, low in cost, strong in killing effect on escherichia coli, salmonella, clostridium welchii and other bacteria, low in clostridium welchii and capable of remarkably decreasing the diarrhea rate of weaned pigs and increasing the growth speed of weaned pigs after being applied to weaned pig feed.

The invention discloses compound pink plumepoppy herb injection for treating diarrhea of calves and a preparation method thereof. The invention aims to provide quick-response compound pink plumepoppy herb injection for treating diarrhea of the calves infected with one or more of Escherichia coli, clostridium welchii, salmonella, mycoplasma and the like and a simple-process and easily-implemented preparation method thereof. 100 liters of the injection comprises 0.2 to 3 kilograms of pink plumepoppy herb extract, 2 to 8 kilograms of mequindox, 1 to 7 kilograms of ofloxacin, 6 to 24 kilograms of sodium salicylate, 20 liters of dimethylformamide, 20 liters of 95 percent ethanol, 5 liters of hydrochloric acid and the balance of injection water. In the compound pink plumepoppy herb injection, the medicaments are reasonably proportioned; an antibacterial spectrum is expanded as big as possible; pathogenic microorganisms are killed pertinently; medicament resistance is effectively reduced; the response is quick; clinical symptoms can be remitted in the occurrence of the diarrhea of the calves; and the conditions of death of the calves, dysontogenesis after the calves are cured and the like are avoided.

The invention provides clostridium welchii. The preservation number of the clostridium welchii is CCTCC NO.M2019204. A microorganism is screened from a domesticated water hyacinth root by an inventor,it is found that the strain has very strong resistance to heavy metals, and the strain is identified as the clostridium welchii by colony morphology, biochemistry and 16S rDNA sequencing analysis. The clostridium welchii can improve the enrichment capacity of water hyacinths to heavy metals including at least one of Cd, Zn, Mn, Ni, Cu and Pb. Meanwhile, IAA and ACC deaminase can be released to promote the growth of the water hyacinths.

The invention discloses a clostridium welchii specific detection primer group and a detection kit. Nucleotide sequences of the specific detection primer group are shown as SEQ ID NO:1 and SEQ ID NO:2respectively, the primer group is adopted for preparing a clostridium welchii specific detection kit, and a novel PCR (polymerase chain reaction) detection method for clostridium welchii is created. By design of special detection primers according to clostridium welchii specific sequences, the quick, specific and sensitive PCR detection method for clostridium welchii epidemiological investigationand clinical infection conditions is created, and accurate detection of clostridium welchii can be realized. The kit is simple and procedural in operation, the method is high in specificity, high sensitivity and objectiveness in result determination are achieved, epidemiological investigation of clostridium welchii, inapparent infection detection and clinical diagonisis can be realized, a promising application prospect and worthiness of large-scale popularization are achieved, and a foundation is laid for further research of clostridium welchii diagnosis, prevention and treatment techniques and the like.

The invention relates to the technical field of feed additives, in particular to a herb-fragrance traditional Chinese medicine feed additive for rabbits. The herb-fragrance traditional Chinese medicine feed additive comprises, by weight, 10-20 parts of herba portulacae, 10-20 parts of taraxacum mongolicum, 3-10 parts of onion, 10-20 parts of radix scutellariae, 10-20 parts of gynostemma pentaphyllum, 3-10 parts of pomegranate bark, 10-20 parts of fructus schizandrae, 3-10 parts of corn stigma, 3-10 parts of watermelon peel, 3-10 parts of glutinous rice root, 10-20 parts of radix trichosanthis, 3-10 parts of lentinula edodes, 3-10 parts of pine needle and 3-10 parts of bone meal. The herb-fragrance traditional Chinese medicine feed additive for the rabbits has the advantages that the feed additive can effectively lower cholesterol, promote intestine and stomach peristalsis, invigorate the stomach and promote digestion, accelerate the growth of the rabbits and enhance the immune function of the rabbits, has antibacterial and anti-virus effects, has a certain preventing and treating effect on the staphylococcosis, clostridium welchii disease, colibacillosis, coccidiosis and rabbit tuberculosis commonly seen in the growth of the rabbits, can guarantee the normal growth of the rabbits, does not contain antibiotics and can improve the quality of rabbit meat, and the rabbit meat has the fragrance of Chinese herb medicine and is unique in flavor.

The invention discloses a quadruple PCR detection primer group for simultaneously detecting shigella, salmonella, clostridium welchii and escherichia coli, which comprises four pairs of primers, namely a shigella upstream primer pair and a shigella downstream primer pair which are respectively SEQIDNO: 1 and SEQIDNO: 2; an upstream primer pair and a downstream primer pair of salmonella are respectively SEQ ID NO: 3 and SEQ ID NO: 4; the clostridium welchii A type primer pair is shown as SEQ ID NO: 5 and SEQ ID NO: 6 respectively; and the primer pairs of the escherichia coli are respectively SEQ ID NO: 7 and SEQ ID NO: 8. A multiple detection method is established for common enteropathogenic bacteria in chicken farms, on the basis of high detection accuracy and good specificity, the detection time is shortened, the detection efficiency is improved, and the multiple detection method is more beneficial to application in large-scale breeding production.

The invention relates to the field of sheep breeding and discloses a method for preventing and treating lamb dysentery. The method includes the following steps that 1, 2-3 weeks before ewes give birth, breeding feed is changed, on the one hand, a large amount of protein, fat, vitamin and mineral matter can be supplemented for growth of the ewes and non-born lambs, on the other hand, the quality of ewe milk is improved, the immunity of the lambs is enhanced, and the infection rate of clostridium welchii is reduced; 2, herb bags are used for carrying out hot compress on the breasts of the ewes, milk production of the ewes can be promoted, meanwhile, growth and propagation of bacterial viruses are avoided, and the lambs safely draw the ewe milk; 3, the growth environment of the lambs is kept warm, comfortable and sanitary, germ breeding is reduced, meanwhile, liquid food is used for feeding the lambs, nutrient substances lacked in breast milk can be supplemented, the gastrointestinal function of the lambs can be improved, and the independent self-immunization function can be established and perfected. By means of the method, the morbidity of lamb dysentery can be reduced by 48%, the cure rate can be increased to 99% or above, and no side effect is generated.

The invention belongs to the field of molecular biological detection. Specifically, the invention relates to detection of bovine diarrhea pathogens. More specifically, the invention relates to a composition for detecting six bovine diarrhea pathogenic pathogens, namely salmonella, clostridium welchii, escherichia coli K99, shigella dysenteriae, campylobacter jejuni and proteus mirabilis. Meanwhile, the invention further provides a kit containing the composition, application of the composition and a method for detecting and typing bovine diarrhea pathogens. The composition is mainly detected in one tube by using multiple fluorescent PCR and melting curve analysis methods, so that different pathogens can be treated differently, and the treatment and prevention are more effective.

The invention discloses a traditional Chinese medical composition for prevention and treatment of A type clostridiosis welchii, which consists of the following components by weight: 10-80 parts of mahonia fortunei, 5-50 parts of pulsatilla chinensis, 3-50 parts of yam, 3-30 parts of semen plantaginis, 3-30 parts of sanguisorba officinalis, 3-30 parts of rheum officinale and 5-45 parts of acalypha australis. The invention also discloses an extract prepared with the composition, a preparation method of the extract, a preparation prepared with the extract and application of the preparation. The composition, the extract and the preparation have broad values in medical application for prevention and treatment of the A type clostridiosis welchii.

The invention discloses a feed additive capable of preventing clostridium welchii disease of rex rabbit, and a preparation method thereof. The feed additive is prepared by performing pulverizing, ethanol extraction, microwave radiation and other processes on cortex fraxini, Chinese pulsatilla root, radix sanguisorbae, herb of oxalis corymbosa, herb of malachium aquaticum, trifolium repens, sweet wormwood herb, cornu caprae hircus, mother-of-pearl, olive oil, diatomaceous earth, sodium bicarbonate, dry yeast, vitamin complex and nicotinic acid. The feed additive has the advantages of reducing the stress response of the rex rabbit and improving the immunocompetence of the rex rabbit; effectively inhibiting aflatoxin caused by feed mildew, prolonging the feed storage time and improving the feed quality, and avoiding the incidence of the rex rabbit caused by feed mildew; regulating body endocrine, improving the gastrointestinal micro-ecological environment of the rex rabbit, and ensuring the quality of rex rabbit hair because of rich plant crude fiber; strengthening the prevention effect of the clostridium welchii disease of the rex rabbi, and reducing the incidence and mortality of the clostridium welchii disease of the rex rabbi so as to reduce the cost and risk of breeding, and increasing the economic benefits of breeding.

The invention belongs to the technical field of agricultural microorganism application, and in particular relates to the screening and application of a probiotic bacillus licheniformis with high production of complex enzymes. The bacillus licheniformis of the invention has obvious antibacterial effect on common livestock and poultry enteropathogenic bacteria such as escherichia coli, staphylococcus aureus and Clostridium welchii. The invention includes the isolation and identification of the bacillus licheniformis strain, the research on biological characteristics and its application as a feed additive for livestock and poultry, and the like. The bacterial strain of the present invention produces cellulase, amylase and protease, is resistant to low pH value of gastric acid and high osmotic pressure of intestinal bile salt, has fast growth rate, strong stress resistance, strong antibacterial property and strong enzyme production ability , can be used to prepare microbial additives for livestock and poultry feed.

The invention relates to a traditional Chinese medicine composition for treating clostridium welchii disease of foxes. The traditional Chinese medicine composition is prepared from 16-30 parts of Chinese pulsatilla root, 12-15 parts of ash bark, 15-20 parts of coptis chinensis, 10-15 parts of semen plantaginis, 15-25 parts of bletilla striata and 10-18 parts of medicine terminalia fruit. The traditional Chinese medicine composition can be powder and is prepared through the following steps: (1), weighing all traditional Chinese medicines according to the formula ratio; (2) smashing all traditional Chinese medicines and screening through a 40-80-mesh sieve; (3), mixing for 10-15 minutes to obtain traditional Chinese medicine powder; (4), sub-packaging the traditional Chinese medicine powder, so as to obtain the finished product, wherein each bag is filled with 100-200 g of the traditional Chinese medicine powder. The traditional Chinese medicine composition has the efficacy of clearing heat, eliminating dampness, cooling blood, stopping dysentery, converging and stopping bleeding. The traditional Chinese medicine composition provided by the invention has an exact curative effect on treating clostridium welchii disease of foxes and the cure rate can reach 84.8%.

The invention discloses nosiheptide flavored chewable tablets, a preparation method thereof and application of the nosiheptide flavored chewable tablets in clostridium welchii disease. The nosiheptideflavored chewable tablets are prepared from, by mass, 1%-10% of nosiheptide, 5% of acidifier, 30%-40% of diluent, 1% of a binder, 30%-40% of a filler, 2%-10% of a disintegrating agent, 5%-20% of a corrigent and 1%-5% of a coating agent. According to the invention, the acidifier is added into the formula to prepare the acidic binder, so that the stability of the main component of nosiheptide is greatly improved, the production of the nosiheptide chewable tablet with controllable quality is facilitated, and the validity period of the product can be obviously prolonged; a carboxymethyl ethyl cellulose aqueous system is used for coating, so that the solubility and bioavailability of nosiheptide are improved; animal tests show that the nosiheptide flavored chewable tablets produced by the invention can be used for effectively treating clostridium welchii disease of dogs.

The invention discloses ternary PCR detection primers and detection method for goat pathogenic bacteria, wherein goatpox virus, clostridium welchii of goat and caprine mycoplasmas can be detected at the same time. The invention relates to three pairs of PCR primers which specifically amplify gene sequences of goatpox virus, clostridium welchii of goat and caprine mycoplasmas, respectively; the three pairs of primers can be amplified in a same PCR reaction tube at the same time to realize multiple detection, so that the detection time is greatly shortened and the method is simple and convenient to operate. Deign of the specific primers ensures high conservation and specificity of the primers and avoids non-complementary pairing or cross-species amplification between the primers and a DNA template. The method disclosed by the invention is small in time consumption, high in efficiency and high in sensitivity.

The invention particularly provides a compound feed for relieving lamb stress. The compound feed is prepared by adopting multiple mineral elements and vitamins as raw materials, compounding bacillus natto, bacillus subtilis and lactic acid bacteria into compound probiotics, and combining the compound probiotics with yeast culture, probiotics, trehalose and skim milk powder. The compound feed has good effects on improving and solving transition stress, feed changing stress, weaning stress, immune stress, management stress and immune stress and preventing enterotoxaemia caused by clostridium welchii; the feeding transition time is short; after transition, the feed intake and the weight increase are not lower than 20% compared with those of a control group; furthermore, supplementary feeding of pasture is not needed; the compound feed can be used in cooperation with conventional feed; and the compound feed can significantly reduce the feeding cost, is safe and reliable, and has wide development value for lamb breeding.

The invention discloses a Clostridium welchii phage, its phage composition and its application. The phage is Clostridium welchii phage PMQ06, which was preserved in the General Microbiology Center of China Committee for Microbial Culture Collection on May 15, 2020. Its deposit number is CGMCC No.19977. The phage has high-efficiency bactericidal activity, wide cleavage spectrum, and high temperature stability, and can be used as an active ingredient in the preparation of medicines, environmental disinfectants, feed additives, and bacteriostatic agents for preventing and treating Clostridioides welchii disease, etc., and can be widely used Applied to various links in the breeding process and product processing and production process, it can quickly, efficiently and safely kill pathogenic Clostridium welchii and even drug-resistant Clostridium welchii, effectively reduce the mortality of diseased chickens, and also has a safe, non-toxic Advantages of residual and no side effects.

The invention discloses an antimicrobial peptide PD22 and its application. The amino acid sequence of the antimicrobial peptide PD22 is shown in SEQ ID NO:1. The antimicrobial peptide PD22 in the present invention has small molecular weight, convenient artificial synthesis and low cost, strong bactericidal effect on bacteria such as Escherichia coli, Salmonella and Clostridium welchii, and low cytotoxicity, and can significantly reduce diarrhea in weaned piglets when used in feed for weaned piglets The advantages of high rate and increased growth rate of weaned piglets.

A disease germ type of colloid Se test paper for testing Clostridium welchii disease consists of plastic base plate, Se labeled pad formed by glass cellulose film with detection region and control region, sampling-adding film, water absorption paper and reaction film formed by nitric acid cellulose film with detection region and control region.

The invention discloses a feed additive capable of preventing a rex rabbit clostridium welchii disease and a preparation method of the feed additive. The feed additive is prepared from rhizoma coptidis, cortex phellodendri, rheum officinale, oxalis corymbosa herb, crickweed herb, white clovers herb, herba artemisiae annuae, goral horns, concha margaritifera usta, olive oil, diatomite, sodium bicarbonate, dry yeasts, comprehensive vitamins and nicotinic acid by carrying out technologies including crushing, extraction with ethanol and microwave radiation. The feed additive can lower the stress response of a rex rabbit and enhance the immunocompetence of the rex rabbit; can effectively inhibit aflatoxin caused by feed mildewing, prolong the conservation time of a feed and improve the quality of the feed so as to prevent the rex rabbit incidence caused by feed mildewing; is rich in plant crude fibers, has the efficiency of regulating an endocrine system, improves the gastrointestinal micro-ecological environment of the rex rabbit and ensures the quality of hairs of the rex rabbit; and can strengthen the effect of preventing the rex rabbit clostridium welchii disease, lower the incidence and the mortality of the rex rabbit clostridium welchii disease, and thus reaching the purposes of lowering the culturing cost and risk and increasing the culturing economic benefit.