The invention discloses a sea cucumber-traditional Chinese medicine extract with kidney-invigorating and essence-replenishing functions as well as a preparation technology thereof and belongs to the technical field of marine-life health food. The sea cucumber-traditional Chinese medicine extract takes fresh sea cucumbers as the raw material and the preparation technology thereof comprises the following steps: sequentially soaking the sea cucumbers by low-concentration alkali and acid solutions after enzymatic degradation, so that the hydrogen bonds between and in the nutrient molecules of the sea cucumbers are cross-linked, the ion branched chains are opened, the crosslink bonds are damaged after soaking of a certain period of time and the nutrient molecules are further hydrolyzed into low-molecular forms such as peptones, peptides, amino acids and the like; thus, the sea cucumbers can be better absorbed by human body. According to the preparation method, the polypeptide yield can be up to higher than 12.0%, the polysaccharide yield is higher than 14% and the degree of hydrolysis is higher than 35%. Moreover, the medicinal and edible Chinese herbal medicines, including radix pseudostellariae, white atractylodes rhizome, Poria cocos, prepared rehmannia roots, ledebouriella roots, roots of herbaceous peony, seeds of Chinese arborvitae, poria with hostwood, astragalus roots, chrysanthemum flowers and the like, are rationally added as accessory materials. The preparation technology is advanced in production process and strong in operability. Furthermore, a special biological fermentation process is utilized, so that the sea cucumbers are improved in flavor and the sea cucumber-traditional Chinese medicine extract is rich in nutrition structure. The sea cucumber-traditional Chinese medicine extract can be used for treating essence deficiency, kidney deficiency, blood deficiency, strength exhaustion, soreness and weakness of waist and knees as well as blood lipid abnormality. Thus, the sea cucumber-traditional Chinese medicine extract is an ideal warm-supplementation health food.