2864 results about "Glutamic acid" patented technology

The invention relates to an engineering bacterium of high-yield ornamental blue as well as a construction method and application of the engineering bacterium, and belongs to the technical field of natural dye synthesis by a biological method. The engineering bacterium capable of highly producing the ornamental blue, disclosed by the invention, expresses icd, bpsA, glnAY405F and gd hA; acnR, yggB, glsK, aceA and ldh are knocked out from the engineering bacteria. According to the application, yggB and aceA genes are knocked out, site-directed mutagenesis (Y405F) is carried out on a glnA gene, a glsK gene is replaced by a glnAY405F gene, an acnR gene is replaced by an icd gene, an ldh gene is replaced by a gdhA gene, and a bpsA gene is integrated, so that the formation of by-products such as lactate and succinic acid in a blue-viewing synthesis process can be blocked; the flow direction of a passage from citric acid to isocitric acid to alpha-ketoglutaric acid to glutamic acid to glutamine is improved, so that the concentration of glutamine in cells is improved, and the yield of the ornamental blue is improved.

The invention discloses a formate dehydrogenase mutant and application thereof in catalytic synthesis of D-mannitol, and belongs to the technical field of bioengineering. According to the formate dehydrogenase mutant provided by the invention, the 18th-site glutamic acid of wild-type formate dehydrogenase with an amino acid sequence shown as SEQ ID NO.2 is mutated into proline, the 57th-site asparagine is mutated into glutamic acid, the 70th-site histidine is mutated into tryptophan, the 235th-site serine is mutated into threonine, and the 316th-site valine is mutated into threonine, so that the formate dehydrogenase mutant is high in enzyme activity and catalytic activity; the coenzyme circulation efficiency during the production of D-mannitol can be obviously improved, so that the yield of D-mannitol can be effectively improved. When the genetically engineered bacterium co-expressed by the formate dehydrogenase mutant and the mannitol dehydrogenase mutant is used for catalyzing 150 g / L fructose to produce D-mannitol, the yield of D-mannitol within 15 h can reach 140 g / L or above.

The invention belongs to the technical field of protein engineering, and discloses a Brazilian sweet protein mutant and application thereof. According to the invention, lysine is used for replacing aspartic acid at the 29th site of Brazilian sweet protein, and / or arginine is used for replacing glutamic acid at the 36th site, and / or alanine is used for replacing aspartic acid at the 50th site, amino acid mutation at the sites is carried out to construct a Brazilian sweet protein mutant, and the Brazilian sweet protein mutant is subjected to heterologous expression in pichia pastoris GS115, so that the Brazilian sweet protein mutant is obtained. And carrying out sweetness performance evaluation on the expressed Brazilian sweet protein mutant. The sweetness property of the double mutant Asp29Lys-Asp50Ala protein is improved by 240% compared with the sweetness property of the Brazilian sweet protein before mutation.

The invention relates to the technical field of enzyme engineering, in particular to an L-glutamate oxidase mutant as well as preparation and application thereof. Through mutation of the 77th amino acid and the 171st amino acid, the active center configuration of the enzyme is optimized, the binding capacity with a substrate and the catalytic conversion efficiency are enhanced, and the enzyme activity is remarkably improved. The mutant disclosed by the invention shows stronger specificity to L-glutamic acid (salt), effectively reduces non-specific reaction and improves the product purity. And in the presence of a high-concentration substrate, the enzyme activity is not inhibited by the substrate. The prepared L-glutamate oxidase mutant with high enzymatic activity can be efficiently expressed in escherichia coli, the problem that the expression quantity of natural enzymes in streptomyces is limited is solved, the acquisition efficiency of the enzymes is greatly improved, and the production cost is reduced. The mutants can be directly applied to biological manufacturing of alpha-ketoglutaric acid, so that the production efficiency of products is remarkably improved.

The invention discloses construction and application of an L-malic acid producing issatchenkia orientalis strain, and belongs to the technical field of microorganisms and the technical field of microbial fermentation and metabolic engineering. Through a metabolic engineering means, a cytoplasm metabolic pathway of an issatchenkia orientalis strain is transformed, and a pyruvate decarboxylase coding gene, glycerol dehydrogenase and lactic dehydrogenase are knocked out; a pyruvate carboxylase gene derived from aspergillus flavus, a malate dehydrogenase gene derived from corynebacterium glutamicum and phosphoenolpyruvate carboxylase derived from escherichia coli are screened and integrated, so that a cytoplasm rTCA cycle synthesis pathway is enhanced; inserting a dicarboxylic acid external transporter gene from schizosaccharomyces cerevisiae at a neutral site GD21, and knocking out endogenous dicarboxylic acid internal transporter; the L-malic acid yield of the modified yeast strain in the fermentation process is remarkably increased and reaches 140.16 g / L. The engineering strain provided by the invention has good industrial application potential in efficient L-malic acid production.

The invention belongs to the technical field of food microorganisms, and relates to lactobacillus plantarum and application thereof in preparation of products for relieving insomnia. The preservation number of the lactobacillus plantarum is GDMCC No: 65061. The lactobacillus plantarum can be applied to efficient fermentation of spina date seed juice, the content of GABA, polypeptide, total phenols and total flavonoids in the spina date seed juice is increased, and the content of spinosin, 6 ''-feruloyl spinosin, jujuboside A, jujuboside B and betulinic acid is increased; the reduction of whole brain coefficient and the increase of liver coefficient caused by long-term insomnia are relieved, the bad condition caused by long-term insomnia in a hippocampus DG region in the brain of the mouse is improved, the sleep time of the mouse is prolonged, and the insomnia symptom of the mouse is relieved; the content of inhibitory neurotransmitters 5-hydroxytryptamine, melatonin and gamma-aminobutyric acid in the hypothalamus of the mouse is increased, the content of excitatory neurotransmitter glutamic acid is reduced, and the reduction of organism immunity and oxidative stress of brain tissues caused by insomnia are relieved.

The invention belongs to the field of bioengineering, and particularly discloses a glutamate oxidase mutant with improved enzymatic activity. According to the invention, site-specific mutagenesis and saturation mutation are carried out on glutamate oxidase coding genes derived from streptomyces mobaraensis, wherein mutations are C536R, P532R, Q378D, V548E and G236D. After the mutant obtained by the invention is induced and purified, the specific enzyme activity of the optimal glutamate oxidase mutant is 1.69 U / mg and is improved by about 30% compared with that of wild bacteria, which shows that the 378-site amino acid residue has great influence on the catalytic action and stability of the enzyme. In addition, the invention also discloses a construction method of the glutamate oxidase mutant with improved enzymatic activity and application of the glutamate oxidase mutant in production of amino acid derivatives such as alpha-ketoglutaric acid. Therefore, the beneficial mutant provided by the invention can lay a good foundation for industrial fermentation production of alpha-ketoglutaric acid and other products.

The invention discloses a high-performance carbonyl reductase mutant and application thereof in synthesis of a series of chiral alcohols. The high-performance carbonyl reductase mutant is obtained by performing the following mutations on an amino acid sequence as shown in SEQ ID NO.2: the 69th alanine mutates into any one of lysine, leucine, aspartic acid or asparagine, and the 69th alanine mutates into any one of lysine, leucine, aspartic acid or asparagine; and / or the 100th glycine is mutated into any one of glutamic acid, serine, lysine or asparagine. Compared with a wild type enzyme, the thermal stability and the catalytic activity of the obtained mutant are remarkably improved, particularly, single point mutants Mut-A69N, Mut-G100E and Mut-G100N and a combined mutant Mut-A69N-G100N show excellent relative enzyme activity, thermal stability and substrate tolerance. The mutant has high catalytic activity and stability to various chiral alcohols, and the contradiction that the traditional carbonyl reductase is easy to inactivate at high temperature and the catalytic efficiency is difficult to achieve at the same time is solved. The invention provides an efficient and stable enzyme catalysis tool for green biological preparation of chiral drug intermediates.

The invention discloses a camel source single-domain antibody GH12 for recognizing a conformation B cell epitope in clostridium difficile glutamate dehydrogenase and application of the camel source single-domain antibody GH12. The nucleotide sequence of the camel source single-domain antibody GH12 is shown as SEQ ID NO: 1, and the amino acid sequence of the camel source single-domain antibody GH12 is shown as SEQ ID NO: 2. The camel source single-domain antibody GH12 disclosed by the invention has the advantages of strong specificity, high sensitivity, good accuracy and the like, and it is proved that the camel source single-domain antibody GH12 can be used for detecting recombinant expressed CD-GDH and endogenous CD-GDH by enzyme-linked immunosorbent assay (ELISA), protein dot blot hybridization (DB) and other methods.

The invention provides a gamma-polyglutamic acid electrostatic compound. The gamma-polyglutamic acid electrostatic compound is formed by compounding gamma-polyglutamic acid and an antibacterial surfactant with positive charges. Compared with the prior art, the gamma-polyglutamic acid electrostatic compound provided by the invention is constructed through electrostatic interaction, not only has good cell compatibility and blood compatibility, but also has excellent broad-spectrum antibacterial property, can be uniformly coated on the surfaces of medical instruments with different base materials, shapes and sizes, is stable in combination, and can be applied to medical instruments with different shapes and sizes. The coating process has universality and is suitable for solving the problem of pollution of current medical instruments, prolonging the service life of the medical instruments and solving the problem of coating stability of the surfaces of inner and outer cavities of complex instruments.

The invention relates to the technical field of chemical synthesis, and particularly provides a preparation method of a semaglutide side chain, which comprises the following steps: step S1, carrying out reaction on octadecanedioic acid mono-tert-butyl ester and trifluoroacetic acid pentafluorophenyl ester to generate a first intermediate; s2, enabling the first intermediate to react with 1-tert-butyl L-glutamic acid to generate a second intermediate; s3, enabling the second intermediate to react with pentafluorophenyl trifluoroacetate to generate a third intermediate; and S4, carrying out an amidation reaction on the third intermediate and 17-amino-10-oxo-3, 6, 12, 15-tetraoxa-9-aza-heptadecanoic acid, so as to generate a semaglutide side chain, and carrying out an amidation reaction on the third intermediate and 17-amino-10-oxo-3, 6, 12, 15-tetraoxa-9-aza-heptadecanoic acid. According to the preparation method disclosed by the embodiment of the invention, the pentafluorophenyl trifluoroacetate is used as the condensation reagent for two times to activate the carboxylic acid, and the amidation reaction is carried out for two times to construct the amido bond after each activation, so that compared with the traditional carbodiimide and urea positive ion type condensation reagent, the chiral racemization in the condensation process can be effectively avoided, and the yield of the product is improved. The condensation chiral racemization impurity can be lower than 0.3%.

The invention relates to the technical field of dye synthesis, and particularly discloses a genetically engineered bacterium for synthesizing N-acetyl blue as well as a construction method and application of the genetically engineered bacterium. The supply capacity of precursor substances glutamic acid and glutamine of the genetically engineered bacterium is high, the fermentation process is simple, the production intensity of the precursor substances glutamine and N-acetylglutamine is improved by cutting off a consumption path, relieving negative feedback inhibition and enhancing the expression level of key genes through the genetically engineered bacterium, and finally the yield of N-acetylblue reaches 41.2 g / L.

The invention relates to the field of polypeptide compositions, in particular to a polypeptide metal chelate and application thereof in the field of cosmetics, and the polypeptide metal chelate is formed by chelating a polypeptide compound and a metal ion compound. The structure of the polypeptide compound is [gamma-Glu] n-AA, n is greater than or equal to 1, and AA is selected from glycine, alanine, valine, leucine, isoleucine, methionine (methionine), proline, tryptophan, serine, tyrosine, cysteine, phenylalanine, asparagine, glutamine, threonine, asparaginic acid, glutamic acid, lysine, arginine or histidine; the polypeptide chelated zinc prepared by the invention has remarkable oil control and anti-inflammatory effects, and can be compounded with other raw materials to form cosmetics with oil control and acne removal effects.

The invention relates to a composition comprising at least one oxidation coupler chosen from 6-hydroxybenzomorpholine of formula (I), one of its addition salts, its solvates and / or the solvates of its salts, at least one oxidation coupler chosen from 2-amino-5-ethylphenol of formula (II), one of its addition salts, its solvates and / or the solvates of its salts, and at least one compound chosen from N, N-dicarboxymethyl glutamic acid, one of its salts, and mixtures thereof. The invention also relates to a process for dyeing keratin fibres, preferably the hair, comprising the application of the composition to said keratin fibres.

The invention discloses a method for improving growth of corynebacterium glutamicum under high-temperature stress, and aims to overcome the problems of abnormal growth and metabolism, even death and the like caused by temperature rise of existing microorganisms in an industrial fermentation production process, adaptive laboratory evolution is adopted, corynebacterium glutamicum ATCC 13032 is used as an original strain, and the corynebacterium glutamicum ATCC 13032 is used as an original strain. And carrying out heat stress subculture for 42.9 days, and separating out the strains HES1 and HES2 with improved high temperature resistance. The whole genome is re-sequenced to obtain mutant elements cspB and gltA which enable the strain to generate high-temperature adaptive change. And carrying out high-temperature test on the abrupt change element. In specific implementation, the two mutants are respectively overexpressed, and in a test, compared with a control strain WT, the 42-DEG C high-temperature tolerance is respectively improved by about 14.4% and 7%. Therefore, the beneficial mutant obtained through adaptive laboratory evolution lays a good foundation for application of corynebacterium glutamicum in industrial high-temperature conditions.

The present invention relates to a kind of silk fibroin hydrogel and preparation method thereof, silkworm raw silk is degummed, sol, dialyzed, filtered, freeze-dried to obtain silk fibroin freeze-dried powder, then redissolved into high concentration silk fibroin solution, filtered and spray-dried into silk fibroin microspheres; silk fibroin microspheres are slowly added to a phosphate buffered saline solution containing γ-polyglutamic acid and glutaraldehyde, stirred and ultrasonically carried out the first cross-linking, then a cross-linking agent solution is added, stirred evenly and ultrasonically, and the second cross-linking is carried out, and the silk fibroin hydrogel is obtained after being rinsed with pure water after being placed for a period of time. In the preparation method of the present invention, a phosphate buffered saline solution containing γ-polyglutamic acid and glutaraldehyde is used as a cross-linking agent and secondary cross-linking is carried out, and the prepared silk fibroin hydrogel silk fibroin has a long retention time, good stability and mechanical strength, and the cross-linking method is simple, and it is suitable for large-scale industrial production.

The invention relates to a preparation method of antibacterial gamma-polyglutamic acid superabsorbent hydrogel. The preparation method comprises the following steps: (1) preparing a gamma-polyglutamic acid solution; (2) dissolving an antibacterial agent in the gamma-polyglutamic acid solution, and fully stirring and uniformly mixing to obtain a mixed solution 1; adjusting the pH value of the mixed solution 1, heating the mixed solution 1 to 60 DEG C, and continuously reacting for 1-2 hours to obtain a mixed solution 2; cooling the mixed solution 2 to room temperature, adding a cross-linking agent, and uniformly mixing to obtain a mixed solution 3; standing the mixed solution 3 at room temperature for 1-3 hours and then carrying out gradient heating reaction to obtain an antibacterial gelatinous product; and (3) dehydrating the antibacterial gelatinous product, and carrying out vacuum negative pressure drying to obtain a dried hydrogel product, namely the antibacterial gamma-polyglutamic acid super absorbent hydrogel. The antibacterial gamma-polyglutamic acid superabsorbent hydrogel prepared by the invention has good biocompatibility, is safe and non-toxic, also has excellent water absorption performance and antibacterial performance, and has a good application prospect.

The invention discloses a UiO-66-(COOH) 2-Glu composite material for efficient recovery of lithium ions and application of the UiO-66-(COOH) 2-Glu composite material. A preparation method of the UiO-66-(COOH) 2 composite material comprises four steps of preparation of UiO-66-(COOH) 2, drying treatment, amino acid modification and separation and purification. The preparation process comprises the following steps: preparing a precursor UiO-66-(COOH) 2-Glu in an aqueous solution system under mild conditions, carrying out functional modification by introducing glutamic acid, and introducing amino groups and carboxyl groups in amino acid to enhance the selective adsorption performance of the UiO-66-(COOH) 2-Glu composite material on lithium ions, thereby obtaining the UiO-66-(COOH) 2-Glu composite material. The preparation process is green, environment-friendly and low in cost, traditional high-temperature and high-pressure conditions are avoided, the practicability of the material is remarkably improved, and the prepared material shows excellent lithium ion selective adsorption performance and shows excellent performance in the field of rapid recovery of lithium ions.

The invention discloses a transcription factor Cgl2988 related to production of 5-aminolevulinic acid based on network model prediction and application, the amino acid sequence of the transcription factor Cgl2988 is as shown in SEQ ID NO.1. The transcription factor Cgl2988 related to production of 5-ALA is simulated and predicted based on a metabolic network model system mcCGL, and experiments prove that the transcription factor Cgl2988 related to production of 5-ALA has the advantages that the transcription factor Cgl2988 related to production of 5-ALA can be used for preparing the transcription factor Cgl2988 related to production of 5-ALA; the expression strain Cgh3-low-cgl2988 of the transcription factor gene cgl2988 in the down-regulated corynebacterium glutamicum is subjected to shake flask fermentation, and the yield of 5-ALA is 5.52 + / -0.19 g / L and is increased by about 1.64 times compared with that of a control strain; the expression strain Cgh3-pX-cgl2988 of the transcription factor gene cgl2988 in the up-regulated corynebacterium glutamicum is subjected to shake flask fermentation, and the yield of the 5-ALA is 5.76 + / -0.11 g / L, which is about 2.84 times higher than that of a control strain.

The invention discloses a low-temperature zinc ion battery high-entropy eutectic electrolyte and a preparation method and application thereof, and relates to the field of batteries, the electrolyte comprises a hydrogen bond donor, a hydrogen bond acceptor and a solvent; the hydrogen bond donor is at least one of methyl sulfonamide, thioformamide, butyramide, asparagine, tert-butylamine, dimethyl propane diamine, oxalyl diamine, benzamide, sulfamide and ethyl sulfonamide; the hydrogen bond receptor is at least one of aspartic acid, glutamic acid, methionine, zinc chloride, zinc perchlorate, zinc fluoborate and zinc acetate; the preparation method comprises the following steps: mixing a hydrogen bond donor and a hydrogen bond acceptor, and heating to convert a solid into a liquid phase; and adding a solvent, and uniformly mixing to obtain the product. The invention also provides a zinc ion battery containing the electrolyte. The high-entropy eutectic electrolyte has the beneficial effects that-NH2 and halogen or carboxyl form hydrogen bonds to perform eutectic reaction, the freezing point of the eutectic solution is lower than that of a reactant, and the solvent is added to improve the confusion degree of the electrolyte, so that the high-entropy eutectic electrolyte is prepared.

The invention discloses corynebacterium glutamicum for producing N-acetylglucosamine and application of the corynebacterium glutamicum, and belongs to the technical field of synthetic biology. Specifically, the corynebacterium glutamicum overexpresses a GNA1 gene from a brewer's yeast S288C controlled by a tacM promoter, a glmS gene from corynebacterium glutamicum S9114 and a yqab gene from escherichia coli K12 MG1655, and simultaneously has five gene defect types of nagA, nagB, ldhA, msccg and msccg2 in a genome and two gene single base mutation types of odhA and glnA, and the codon of the glnA for coding the 405th amino acid is mutated from TAC to TTC. By blocking excretion of glutamic acid of corynebacterium glutamicum and enhancing conversion of glutamic acid to glutamine, the supply capacity of a precursor of corynebacterium glutamicum is enhanced, and an optimal way for synthesizing N-acetylglucosamine is obtained, so that efficient production of N-acetylglucosamine in corynebacterium glutamicum is realized, and the yield of N-acetylglucosamine in corynebacterium glutamicum is increased. The method has important economic value and social significance.

The invention relates to the technical field of plant cultivation, and discloses a method for improving low-temperature and low-oxygen composite stress resistance of tobacco seedlings by exogenous amino acids, which comprises the following steps: selecting tobacco seedlings in a two-leaf and one-core stage of floating seedling raising; preparing an L-pyroglutamic acid mother solution; performing exogenous treatment on the selected tobacco seedlings in the two-leaf and one-core stage by using the prepared L-pyroglutamic acid mother liquor; after treatment is completed under the low-temperature and low-oxygen condition for 3 days, taking a picture of a tobacco seedling, recording phenotype, sampling roots, and measuring root length and root activity; according to measured values under the treatment of adding different volumes of L-pyroglutamic acid mother liquor with the concentration of 1g / L, data is sorted by software, difference significance analysis is carried out by data processing software, and comprehensive evaluation is carried out by a multi-character fuzzy membership function method. The application comprehensively evaluates the effect of the L-pyroglutamic acid in improving the low-temperature and low-oxygen stress resistance of the tobacco seedlings, and provides important theoretical basis and practical guidance for improving the crop stress resistance in agricultural production.

The invention relates to the technical field of genetic engineering, and discloses a genetically engineered bacterium for producing L-arginine as well as a construction method and application of the genetically engineered bacterium. Escherichia coli is taken as a starting strain, and the following treatment is performed: overexpressing genes gdhA and gdh of glutamate dehydrogenase, promoting generation of glutamic acid, overexpressing and coding arginine transporter gene argO to enhance extracellular transport of arginine, introducing exogenous argJ to replace a linear synthesis pathway in large intestines, improving the utilization rate of acetyl coenzyme A and accelerating the synthesis efficiency of arginine; a glutamine synthetase gene glnA is over-expressed, and glutamine synthesis is enhanced; the pckA is overexpressed to promote ATP synthesis; meanwhile, speA and adiA genes for coding the arginine decarboxylase are deleted, so that further synthesis of putrescine from L-arginine is inhibited, and the L-arginine accumulation capability of the strain is improved. Through the combined action of the treatment, a genetically engineered bacterium with extremely high L-arginine production yield is finally obtained, and the shake flask fermentation yield can reach 13.7 g / L.

Provided is a glutamic or glutaric acid-based ionizable lipid compound of Formula (I) or a pharmaceutically acceptable salt thereof. The compound can be used to obtain lipid nanoparticles. In some embodiments, the lipid nanoparticle can comprise (a) from about 40 to about 100 mol % of the compound of Formula (I); (b) from 0 to about 20 mol % of a neutral lipid; (c) from 0 to about 50 mol % of a helper lipid; (d) from 0 to about 5 mol % of a polymer-conjugated lipid; and (e) from 0 to about 10 mol % of a hydrophobic component; wherein the mol % are based on the total lipids present in the nanoparticle. In some embodiments, the ionizable lipid compound is a glutamic acid-based ionizable lipid compound.

The invention discloses a bullfrog extracted biological collagen composition and a preparation method thereof in the technical field of collagen. The bullfrog extracted biological collagen composition comprises the following components in parts by weight: 1-1.5 parts of hydrophobic bullfrog source collagen peptide, 0.6-0.8 part of hydrophobic bullfrog source elastin peptide, 1-1.5 parts of polyglutamic acid and 1-1.5 parts of polylysine. Collagen and elastin in bullfrog skin and bullfrog cartilage are extracted in a combined mode through a three-enzyme co-production method, and after bullfrog source collagen peptide and bullfrog source elastin peptide are subjected to hydrophobic modification, polyglutamic acid and polylysine are added to form a collagen combination. The stability, the medicine carrying capacity and the slow release capacity of the composition can be improved.

Certain C. glutamicum strains overexpress genes coding for enzymes having the function of aspartate-semialdehyde dehydrogenase, aspartate aminotransferase, dihydrodipicolinate synthase, dihydrodipicolinate reductase, diaminopimelate decarboxylase, aspartatokinase and diaminopimelate dehydrogenase. The C. glutamicum strains also express NAD(P)(+) transhydrogenase subunit alpha PntA and NAD(P)(+) transhydrogenase subunit beta PntB of Corynebacterium urealyticum. A method is developed for the fermentative production of L-lysine using such C. glutamicum strains.

The present invention provides a phycocyanin-collagen composite hydrogel and its preparation method and application. The present invention utilizes transglutaminase to catalyze the combination of phycocyanin and collagen to form a new, more stable collagen; transglutaminase acts between protein molecules, utilizes lysine and glutamic acid in phycocyanin and collagen to transfer acyl groups, and cross-links to form new chemical bonds, thereby enhancing the binding strength of phycocyanin and collagen; the hydrogel prepared by the present invention can be used to prepare medical materials and combined with skin injury sites to solve the problem that traditional dressings are easy to adhere to wounds and easily penetrated by tissue fluid and become infected. In addition to ensuring basic air permeability, it reduces the adhesion of the dressing, increases moisture retention and antioxidant properties, and avoids secondary damage when replacing a new dressing. The hydrogel prepared by the present invention has a certain antioxidant capacity and can be used to prepare products with antioxidant effects.

The invention discloses corynebacterium glutamicum for producing L-isoleucine and application of the corynebacterium glutamicum. The strain is obtained by performing ultraviolet mutagenesis and structural analogue resistance screening on corynebacterium glutamicum ATCC13869. According to the method, the L-isoleucine can be produced through direct fermentation of glucose, the yield of the L-isoleucine can reach 51.69 g / L, the conversion rate can reach 28.5%, the production efficiency is improved, meanwhile, the main raw material glucose used for fermentation is low in price and easy to obtain, the method conforms to the green food safety production concept, and industrial production of the L-isoleucine is facilitated.

An artificial synthesis method for malonyl-CoA and use thereof are provided. By means of heterologous expression of an aminotransferase and a malonyl-CoA reductase, an artificial synthesis pathway for synthesizing malonyl-CoA by using β-alanine (β-ala) as a precursor is constructed as follows: firstly, under catalysis of a transaminase, β-ala transfers amino groups to α-ketonic acid (such as pyruvic acid, oxaloacetic acid, or α-ketoglutaric acid, etc.), to form an intermediate product 3-oxopropanoate and a corresponding amino acid; the 3-oxopropanoate generates malonyl-CoA under the action of the malonyl-CoA reductase. This pathway addresses the defects of the natural malonyl-CoA synthesis pathway, such as low carbon utilization, consumption of energy substance ATP, release of greenhouse gas CO2, and strict regulation of pathway enzymes, a pyruvate dehydrogenase (PDH) and an acetyl-CoA carboxylase (ACC), thereby achieving high yielding of products using malonyl-CoA as a precursor, including flaviolin, octanoic acid, phloroglucinol, pentadecaheptaene, natamycin, and spinosad.