The present invention relates to methods for diagnosis of chronic prostatitis / chronic pain pelvic syndrome (CP / CPPS). We have found specific biomarkers that are present in higher concentrations in patients that have chronic prostatitis / chronic pain pelvic syndrome (CP / CPPS) as compared to subjects that have no symptoms of CP / CPPS. In particular, uromodulin (THP), aminopeptidase N (AMPN), dipeptidylpeptidase IV (CD26), neprilysin (NEP), zinc-α-2-glycoprotein (ZA2G) and alkaline phosphatase (ALP) were found to be present at higher concentrations in CP / CPPS patient urine that is voided after prostatic message. Accordingly, the invention is directed to methods for diagnosis of CP / CPPS by monitoring the levels of at least one of these proteins in post-prostatic massage urine, as well as to diagnostic kits designed for diagnosis of CP / CPPS.