1513 results about "Transcriptional factor" patented technology

The present invention relates to a soybean transcription factor GmERF416 and a coding gene thereof, and a use thereof in regulating a plant yield and / or salt tolerance / alkali tolerance, and further relates to a method for cultivating plants having an increased yield and / or salt tolerance / saline-alkali tolerance, and prepared plants, such as soybeans, having an increased yield and / or salt tolerance / saline-alkali tolerance.

The invention belongs to the field of plant genetic engineering, and discloses application of a vitis amurensis cold-resistant gene VaWRKY65 in plant cold-resistant genetic improvement, the VaWRKY65 gene is a transcription factor separated and cloned from extremely cold-resistant vitis amurensis, and the sequence of the VaWRKY65 gene is shown as SEQ ID NO.1. The invention further discloses a preparation method of the vitis amurensis cold-resistant gene VaWRKY65. The gene is used for constructing an overexpression and interference vector, the overexpression and interference vector is respectively introduced into arabidopsis thaliana and vitis amurensis through agrobacterium tumefaciens-mediated genetic transformation, and biological function verification of the obtained transgenic plant shows that the cloned VaWRKY65 gene has the function of improving the cold resistance of the plant. The discovery of the gene provides a new gene resource for plant stress resistance molecular design breeding and provides a new genetic resource for implementation of green agriculture and water-saving agriculture, and development and utilization of the genetic resource are beneficial to reduction of agricultural production cost and realization of environmental friendliness.

The invention discloses a rice nitrogen response regulation and control network analysis and breeding target identification system and method based on multi-omics data. According to the system, organic combination of regulation and control network construction based on single or multiple varieties of materials, key transcription factor recognition and accurate positioning of regulation and control areas where transcription factors play roles is achieved through an expression-chromatin accessibility correlation research method, and cis-trans effect distinguishing of the regulation and control areas is achieved through a deep learning model. The method comprises the following steps: carrying out nitrogen starvation pretreatment on rice, then carrying out nitrogen resupply, collecting a root sample, and carrying out ATAC-seq and RNA-seq sequencing; an eCAAS method is adopted to construct a regulation and control network, and key transcription factors are identified and accurately positioned; the chromatin accessibility difference of different varieties is predicted through a deep learning model, the cis-action effect and the trans-action effect are distinguished, an upstream transcription factor target is provided for genes dominated by the trans-effect, and haplotype and editable regulatory region targets available for direct breeding are provided for genes dominated by the cis-effect.

The invention discloses application of a TaLBD30 protein and a coding gene thereof in regulating and controlling a wheat plant type. The invention belongs to the technical field of biology, and particularly relates to application of TaLBD30 protein and a coding gene thereof to regulation and control of wheat plant types. The protein is any one of the following proteins: A1) a protein with an amino acid sequence as shown in SEQ ID No: 1; a2) a protein which is obtained by substitution and / or deletion and / or addition of amino acid residues on the protein of A1), has 80% or more of identity with the protein of A1) and has the same function as the protein of A1); a3) a fusion protein obtained by connecting a protein tag to the N terminal or / and the C terminal of A1) or A2). TaLBD30 is a transcription factor with transcription inhibition activity, and compared with a wild type, the TaLBD30 gene overexpression shows that after TaLBD30 overexpression, the wheat plant height and ear length are remarkably reduced.

The invention belongs to the technical field of plant molecular biology, and particularly relates to an oilseed rape flowering negative regulation transcription factor BnMYB615, a cloning method, a BnMYB615 gene overexpression vector, a BnMYB615 gene knockout vector, a host cell, a flowering phase delay transgenic plant and application of a flowering phase advance gene editing plant. A nucleotide sequence of the rape transcription factor BnMYB615 is shown as SEQ ID NO.1, an amino acid sequence of the rape transcription factor BnMYB615 is shown as SEQ ID NO.2, an overexpression vector sequence of the rape transcription factor BnMYB615 is shown as SEQ ID NO.3, and a gene knockout vector sequence of the rape transcription factor BnMYB615 is shown as SEQ ID NO.4. The overexpressed BnMYB615 can obviously delay the flowering time of plants, and can prolong the growth season and the photosynthetic time of the plants and obtain higher yield when being applied to crops taking nutritive organs as harvest objects; after the BnMYB615 gene is knocked out, the flowering time of the plant is advanced, and when the BnMYB615 gene is applied to crops taking seeds or fruits as harvest objects, the growth period of the crops can be shortened, the multiple cropping index can be improved, meanwhile, the influence of adverse climate can be avoided, and the product quality can be improved.

The invention provides a plant salt tolerance related transcription factor TaVOZ1 as well as a coding gene and application thereof, and belongs to the technical field of functional genes. The TaVOZ1 is related to plant salt tolerance and has an amino acid sequence as shown in SEQ ID No. 1, and a gene for coding the protein has a nucleotide sequence as shown in SEQ ID No. 2. The plant salt tolerance can be regulated by regulating the content or activity of the protein or the expression quantity of the gene, for example, when the gene is over-expressed, the plant salt tolerance is enhanced, and when the gene is knocked down, the salt tolerance is weakened. The TaVOZ1 disclosed by the invention can be combined with 5 '-CTTCT-3'or a reverse complementary sequence 5'-AAGAAG-3 'in a downstream target gene promoter to activate the expression of a stress response gene so as to improve the salt tolerance of a plant, so that the TaVOZ1 can be used for culturing a salt-tolerant wheat variety.

Disclosed herein are compositions and methods for modulating T cells. For example, the compositions and methods may be used to increase memory T cells. The compositions and methods may increase the expression or protein level of a transcription factor selected from THAP6, DMRT3, MEF2B, PAX2, ATOH7, KLF2, KLF1, TWIST1, NKX6, and FEV, or a combination thereof. The compositions and method may be used in combination with Adoptive T Cell Therapy (ACT) to enhance the ACT.

The invention discloses a tomato fruit cracking key regulation gene identification method, through the method, 41 key genes for regulating tomato cracking are determined and obtained for the first time, and the genes mainly participate in plant hormone synthesis, cell wall metabolism, wax and cuticle metabolism, absorption and transportation of mineral elements and water, and some transcription factors. Two regulation networks are established and comprise an ethylene-cell wall metabolic pathway and a transcription factor-water channel-mineral element. The research combines high-throughput sequencing and molecular biological techniques, enriches the cracking mechanism of tomato fruits on transcription and translation levels, and thus, has important scientific significance and application prospects.

The invention discloses a corn NAC transcription factor and application thereof in enhancing disease resistance. The corn NAC transcription factor can endow transgenic rice with wide resistance and does not affect the rice yield, and the amino acid sequence of the corn NAC transcription factor is SEQ ID No.2. The sequence of the coding gene of the gene is SEQ ID No.1. Research shows that the corn NAC transcription factor is located in a cell nucleus, the resistance of rice to rice blast caused by magnaporthe oryzae and rice bacterial leaf blight caused by Xanthomonas oryzae can be improved by overexpression of the corn NAC transcription factor in rice, meanwhile, the agronomic traits of the rice are evaluated, it is found that the gene does not affect the ear length and the grain length of the rice, and the corn NAC transcription factor can be used for improving the rice resistance to rice blast caused by magnaporthe oryzae and the rice bacterial leaf blight caused by Xanthomonas oryzae. Thousand seed weight and single plant yield.

The invention is applicable to the technical field of bioengineering, and provides a coding gene for enhancing iron deficiency stress tolerance of plants and application. According to the soybean GmMYB14 transcription factor disclosed by the invention, the problem that the growth of soybeans is limited in an iron-deficient environment, particularly calcareous soil and soda saline alkali soil, is solved by excavating and applying the encoding gene (the nucleotide sequence is shown as SEQ ID No.1) of the soybean GmMYB14 transcription factor. Experiments prove that after the gene is over-expressed in arabidopsis thaliana and soybean, the tolerance of plants to iron deficiency stress can be remarkably enhanced. The invention provides a key gene resource for molecular breeding: the GmMYB14 effectively promotes the absorption and utilization of plants on iron by regulating and controlling the metabolic pathway of phenylpropane, not only can improve the planting adaptability of soybeans in low-iron soil, but also can improve the yield and quality of the soybeans, provides powerful technical support for enlarging the planting area of the soybeans in iron-deficient soil regions, and has a wide application prospect. The method is of great significance in relieving insufficient soybean productivity in China and improving the self-sufficiency rate.

The invention discloses a coumarin synthetic gene in black bamboo and application thereof, and belongs to the technical field of molecular biology. The invention provides transcription factors PnMYB4 and PnMYB4-1 for promoting synthesis of coumarin in black bamboos and application of the transcription factors PnMYB4 and PnMYB4-1. The PnMYB4 and the PnMYB4-1 belong to an MYB transcription factor family, the length of a complete coding frame of the PnMYB4 is 762 basic groups, and the coded protein contains 253 amino acids; the length of a complete coding frame of the PnMYB4-1 is 744 basic groups, and the coded protein contains 253 amino acids. The coumarin substances in arabidopsis thaliana, which are respectively overexpressed by PnMYB4 and PnMYB4-1, are obviously increased, and in addition, the expression levels of coumarin synthetic genes such as AtPAL1, At4CL1 and AtC4H in overexpressed plants are obviously increased.

The invention relates to a method for regulating and controlling gene expression of lactic acid bacteria in shrimp acid fermentation based on systematic biology. The method comprises the following steps: constructing a gene regulation network of lactic acid bacteria by using whole genome data and a high-throughput sequencing technology comprising RNAseq; through clustering analysis and network reconstruction, transcription factors and enzymes of key metabolic pathways of lactic acid generation, acid resistance and glycometabolism are identified, and a dynamic regulation and control model of gene expression is established; a GeneNet or Cytoscape software tool is used, genomics, transcriptomics and metabonomics data of lactic acid bacteria are fused in a multi-layer network, a metabolism regulation and control system is constructed, and the metabolism regulation and control system comprises gene expression regulation and control and real-time change of metabolic flow; the method comprises the following steps: constructing a metabolic flux dynamic model of lactic acid bacteria based on a metabolic flux analysis MFA and a dynamic system modeling technology, and analyzing a mutual relation between a lactic acid generation path and a byproduct generation path; fBA is analyzed through flow equilibrium, and the metabolic network of lactic acid bacteria is optimized.

The invention discloses a grape seed development gene VvPHERES1 and an application of the grape seed development gene VvPHERES1. The nucleotide sequence of the developmental gene VvPHERES1 is as shown in SEQ ID NO: 1. The amino acid sequence of the TypeI MADS transcription factor is as shown in SEQ ID NO: 2. According to the invention, the grape seed development related gene VvPHERES1 is cloned for the first time, and the gene function of the grape seed development related gene VvPHERES1 is researched. The method comprises the following steps: constructing a pCAMBIA2300-VvPHERES1 overexpression vector, and carrying out heterologous transformation on a Micro-Tom tomato plant. By comparing the change of seed characters in the overexpressed transgenic tomato and the wild type plant, the result shows that the number of seeds in the overexpressed transgenic tomato is obviously increased. The invention provides a gene related to regulation and control of grape seed development, and a basis is provided for seed selection of new seedless grape varieties.

The invention discloses a transcription factor Cgl2988 related to production of 5-aminolevulinic acid based on network model prediction and application, the amino acid sequence of the transcription factor Cgl2988 is as shown in SEQ ID NO.1. The transcription factor Cgl2988 related to production of 5-ALA is simulated and predicted based on a metabolic network model system mcCGL, and experiments prove that the transcription factor Cgl2988 related to production of 5-ALA has the advantages that the transcription factor Cgl2988 related to production of 5-ALA can be used for preparing the transcription factor Cgl2988 related to production of 5-ALA; the expression strain Cgh3-low-cgl2988 of the transcription factor gene cgl2988 in the down-regulated corynebacterium glutamicum is subjected to shake flask fermentation, and the yield of 5-ALA is 5.52 + / -0.19 g / L and is increased by about 1.64 times compared with that of a control strain; the expression strain Cgh3-pX-cgl2988 of the transcription factor gene cgl2988 in the up-regulated corynebacterium glutamicum is subjected to shake flask fermentation, and the yield of the 5-ALA is 5.76 + / -0.11 g / L, which is about 2.84 times higher than that of a control strain.

The invention provides a cell specific transcription factor regulatory network analysis method and a visualization platform, and relates to the technical field of bioinformatics, the method comprises the following steps: constructing a gene regulatory network through a GRNBoost2-cisTarget-AUCell-Cell GRN workflow based on a transcription factor in combination with a motif database; screening a direct regulation relationship in combination with the database, and calculating an activity score of a regulator in each cell; based on activity scores and cell type annotation results, grouping the single cell data by using a unified manifold approximation and projection (UMAP) dimensionality reduction method and a Leiden clustering algorithm, and displaying the following results through an interactive visualization tool: a cell clustering UMAP graph, performing color marking according to cell types; a UMAP graph and a heat map of transcription factor regulator activity; according to the visual map of the gene regulation and control network, transcription factors and target genes are distinguished through node shapes, and regulation and control relations are marked through line weights and colors. According to the invention, an accurate regulation and control network can be provided.

The invention belongs to the technical field of plant gene engineering, and particularly relates to an OsNUC1 gene and application of a gene mutant constructed by the OsNUC1 gene to regulation and control of rice plant height, leaf length and panicle type. The regulation is to regulate the plant height and leaf growth of the rice and influence the shape of the ear part. Compared with the prior art, the OsNUC1 gene mutant has the following advantages: (1) the OsNUC1 is directionally edited by utilizing a CRISPR / Cas9 gene editing technology, an OsNUC1 gene mutant rice material is obtained, the biological phenotype of the OsNUC1 gene mutant rice material is investigated, the phenotype investigation on T1-generation seedlings of the OsNUC1 shows that the plant heights and the leaf lengths of one-week-old and two-week-old seedlings of the mutant are obviously higher than those of wild seedlings, the leaf widths have no obvious difference, and the OsNUC1 gene mutant rice material has a good application prospect; the result shows that the OsNUC1 can regulate the plant height and the leaf growth of the rice and influence the shape of the ear part. (2) it is proved that the nucleolin gene OsNUC1 is negatively regulated by a transcription factor OsARF7, and the phenotypes such as the plant height, the leaf length, the grain length and the grain width of the OsNUC1 / OsARF7 mutant are more obvious than those of the OsNUC1 mutant. The invention further proves the application of the OsNUC1 gene in regulation and control of rice plant height and leaf growth.

The invention belongs to the technical field of molecular biology, and particularly relates to a CeNF-YA3 gene, an expression vector and application of the CeNF-YA3 gene in vegetable fat regulation and control. The nucleotide sequence of the CeNF-YA3 gene is as shown in SEQ ID NO.1. After the CeNF-YA3 gene is over-expressed, the grease content of arabidopsis seeds and leaves can be remarkably increased; in addition, the invention also provides a series of vectors containing the CeNF-YA3 gene for subcellular localization, yeast hybridization and plant overexpression for the first time, and proves that the CeNF-YA3 protein has a transcriptional activation function, is localized in a cell nucleus and accords with the basic characteristics of transcription factors for the first time. Therefore, the technical scheme of the invention provides scientific guidance for the application of the CeNF-YA3 gene and the protein thereof.

The invention relates to the technical field of cancer risk prediction, in particular to a deep learning cancer risk prediction method and system based on a methylation sequence. The method comprises the following steps: acquiring DNA methylation sequencing data and reference genome annotation data in a blood sample of a cancer patient; performing functional region classification processing on the DNA methylation sequencing data according to the reference genome annotation data to obtain promoter subregion methylation data, enhancement subregion methylation data and transcription factor binding site methylation data; performing dynamic sequence similarity calculation processing on the methylation data of the promoter region to obtain a first feature vector containing importance weight of the promoter region of the cancer-related gene; the methylation sites are classified according to the functions of the methylation sites in the transcriptional regulation pathway, so that the biological interpretation of the characteristics is improved, and the synergistic effect among different functional regions can be captured.

The invention relates to cloning of a stress-resistant gene LpbHLH144 of Lilium pumilum DC (Lilium pumilum DC.) and application of the stress-resistant gene LpbHLH144 of Lilium pumilum DC. Comprising the following steps: (1) cloning a target gene; (2) constructing an expression vector; and (3) detecting physiological indexes of the transgenic plant. According to the experiment, lilium tenuifolium is taken as a research object, and the LpbHLH144 gene is cloned by applying an RT-PCR technology; transforming tobacco by constructing an overexpression vector; the functions of overexpression of the LpbHLH144 for improving the saline-alkaline and drought stress of the tobacco are identified through transgenic tobacco, the functions of the lilium tenuifolium bHLH gene when the lilium tenuifolium bHLH gene is subjected to adversity stress are disclosed, a foundation is laid for further application of the lilium tenuifolium in lily breeding, and a theoretical basis is provided for the functions and effects of bHLH transcription factors.

The invention provides a patchouli transcription factor PcIDD2 and application of the patchouli transcription factor PcIDD2 in forward regulation of biosynthesis of patchouli ketone. The patchouli transcription factor PcIDD2 has an amino acid sequence as shown in SEQ ID NO.1; the transcription factor is a potential transcription factor with a function of promoting biosynthesis of the patchouli ketone, and has important significance in promoting efficient synthesis of the patchouli ketone.

The invention discloses application of an apple MdMYS1 gene in regulating and controlling the wax content of plant fruits and leaves, and belongs to the technical field of plant genetic engineering. According to the invention, the MdMYS1 gene with high expression quantity in apple varieties with more waxiness is separated, and the nucleotide sequence of the MdMYS1 gene is shown as SEQ ID NO. 1. Through subcellular localization, the transcription factor expressed by the MdMYS1 gene is found to be localized on a cell nucleus. Experimental results show that overexpression of the MdMYS1 gene can significantly increase the wax content of the apple fruits and leaves by promoting biosynthesis of wax, which indicates that the MdMYS1 gene plays a key role in regulation and control of the wax content of the apple fruits and leaves. The invention provides an efficient and rapid way for apple breeding, provides a gene material for improving apple quality, and has a wide application prospect in improving economic benefits and ecological benefits of apple planting.

The invention relates to the technical field of plant biology, and discloses application of a transcription factor OsEPR1 as a negative regulatory factor in increasing the content of volatile metabolites in rice, and the amino acid sequence of the transcription factor OsEPR1 is shown as SEQ ID NO.41. Through overexpression of the OsEPR1 gene driven by a constitutive promoter in rice, the content of a plurality of volatile metabolites in rice grains is remarkably reduced, including but not limited to volatile metabolites in a lipoxygenase pathway; the OsEPR1 is used as a negative regulatory factor to inhibit the synthesis of a plurality of volatile metabolites including a rice lipoxygenase pathway, so that the synthesis of the plurality of volatile metabolites of the rice can be improved by knocking out the OsEPR1 gene in the rice, and the OsEPR1 gene can be applied to rice fragrance quality improvement research.

The invention discloses application of a transcription factor BnaMYB52 in regulation and control of oil content and lignin content of rape seeds. The amino acid sequence of the transcription factor BnaMYB52 is shown as SEQ ID NO.5, or other amino acid sequences which are derived from the amino acid sequence shown as SEQ ID NO.5 through substitution and / or deletion and / or addition of one or more amino acid residues and have the same protein function. An agrobacterium tumefaciens-mediated genetic transformation method is used for performing overexpression and gene editing on the BnaMYB52 gene respectively, and it is found that the BnaMYB52 gene negatively regulates and controls the oil content of rape seeds and positively regulates and controls the lignin content. The method disclosed by the invention has very important application prospects in high-oil-content breeding of the rape and quality improvement of the rape cake meal.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a wheat transcription factor TaWRKY22 and a coding gene thereof in regulation and control of plant stripe rust resistance. The function of the wheat transcription factor TaWRKY22 is researched through molecular biology and genetics methods, it is found that knockout of the TaWRKY22 gene significantly improves the resistance of wheat to stripe rust, overexpression of the TaWRKY22 gene reduces the resistance of wheat to stripe rust, and then it is indicated that the wheat transcription factor TaWRKY22 plays a negative regulation role in wheat stripe rust resistance. Therefore, the TaWRKY22 gene is subjected to targeted modification through a gene editing technology, a new genetic material and a molecular breeding strategy can be provided for wheat stripe rust resistance breeding, and an important material and technical support are provided for breeding of wheat disease-resistant varieties.

The invention relates to a genome regulatory element analysis method based on deep learning. The method comprises the following steps: acquiring a genome DNA sequence and DNA shape information; on the basis of a shared hybrid neural network, transcription factor binding site features are extracted from a genome sequence, and binding specificity prediction is carried out aiming at the sequence level; aiming at the specificity of transcription factor binding, accurately positioning a transcription factor binding site on a nucleotide level by adopting a full convolutional neural network coupled with different models, and identifying a motif; for a chromosome three-dimensional genome, a chromatin ring is recognized in the three-dimensional whole genome based on a deep learning model, the relationship between the chromatin ring and regulatory elements in all cell lines is analyzed, and the chromatin ring represents cell type specificity and co-enrichment of transcription factor motifs among different cell lines and species. Compared with the prior art, the method has the advantages that the regulatory elements of the regulatory mechanism can be identified and analyzed by utilizing multiple information sources, and the accuracy of predicting the whole genome chromatin ring is effectively improved.

The invention discloses application of a transcription factor BnaMYB52 in regulation and control of drought resistance of rape, and relates to the technical field of biology. The amino acid sequence of the transcription factor BnaMYB52 is as shown in any one of SEQ ID NO. 5 to 8. According to the invention, the negative regulation effect of the transcription factor BnaMYB52 in the drought resistance of the rape is disclosed for the first time, and a brand new molecular target and a genetic improvement strategy are provided for improving the drought resistance of the rape. Through in-depth study, it is found that knockout of the BnaMYB52 gene can significantly enhance the tolerance of rape plants to the drought environment. In addition, a new drought-resistant rape germplasm obtained after genetic modification of the gene shows excellent adaptive capacity in a drought stress environment. The technical breakthrough not only helps to solve practical problems faced by rape planting in arid regions, but also lays a solid theoretical foundation and technical support for cultivating high-yield and high-quality rape varieties adapting to extreme climate conditions.

The invention belongs to the technical field of agricultural biology, and particularly relates to a transcription factor StBSB with a regulation effect on steroid glycoalkaloid accumulation of potatoes and application of the transcription factor StBSB, the transcription factor StBSB is encoded by a potato gene Soltu.DM.05G001620, and the nucleotide sequence of the potato gene Soltu.DM.05G001620 is shown as SEQ ID NO.1. The invention further discloses a preparation method of the transcription factor StBSB. According to the application, a new insight is provided for understanding a transcriptional regulation mechanism of SGAs in solanaceae plants, and a key theoretical basis and genetic resources are provided for directionally improving the content of anti-nutritional compounds through genetic means.

The invention discloses a panax notoginseng MYB transcription factor gene PnMYB8, the nucleotide sequence of the gene PnMYB8 is as shown in SEQ ID NO: 1, an MYB transcription factor is encoded, molecular biology and functional genomics related technologies prove that the PnMYB8 gene has the capability of improving fungal infection resistance of plants, the PnMYB8 gene is constructed on a plant expression vector and transferred into tobacco for overexpression, and the yield of the panax notoginseng MYB transcription factor gene PnMYB8 is improved. Experimental results show that the resistance of the transgenic tobacco overexpressed with the PnMYB8 to the tryptophan destructor (Cylindrocarpon destructor), the fusarium latericum (Fusarium latericum), the fusarium equiseti (Fusarium equiseti) and the phoma herbarum (Phoma herbarum) is obviously enhanced, and the resistance of the transgenic tobacco overexpressed with the PnMYB8 to the tryptophan destructor (Fusarium latericum) is obviously enhanced.

The invention belongs to the technical field of biotechnology and genetic engineering, and particularly relates to application of recombinant RASA3 in preparation of a medicine for preventing and treating viral pneumonia. The recombinant RASA3 is used for promoting autophagy degradation of a transcription factor CEBP / beta protein and reducing the expression level of the transcription factor CEBP / beta protein, so that generation of inflammatory factors such as IL-6 is reduced, and inflammatory damage caused by excessive antiviral innate immune response in the virus infection process is improved. The invention provides a novel treatment strategy for inflammatory diseases caused by RNA viruses such as influenza and respiratory syncytial virus infection.

The present invention relates to methods and compositions for reducing the immunogenicity of chimeric Notch receptors, and specifically to transcription factors useful for controlling gene expression delivered to tissues by such chimeric Notch receptors.