895 results about "Transcriptional factor" patented technology

The present invention relates to a soybean transcription factor GmERF416 and a coding gene thereof, and a use thereof in regulating a plant yield and / or salt tolerance / alkali tolerance, and further relates to a method for cultivating plants having an increased yield and / or salt tolerance / saline-alkali tolerance, and prepared plants, such as soybeans, having an increased yield and / or salt tolerance / saline-alkali tolerance.

The invention discloses a rice nitrogen response regulation and control network analysis and breeding target identification system and method based on multi-omics data. According to the system, organic combination of regulation and control network construction based on single or multiple varieties of materials, key transcription factor recognition and accurate positioning of regulation and control areas where transcription factors play roles is achieved through an expression-chromatin accessibility correlation research method, and cis-trans effect distinguishing of the regulation and control areas is achieved through a deep learning model. The method comprises the following steps: carrying out nitrogen starvation pretreatment on rice, then carrying out nitrogen resupply, collecting a root sample, and carrying out ATAC-seq and RNA-seq sequencing; an eCAAS method is adopted to construct a regulation and control network, and key transcription factors are identified and accurately positioned; the chromatin accessibility difference of different varieties is predicted through a deep learning model, the cis-action effect and the trans-action effect are distinguished, an upstream transcription factor target is provided for genes dominated by the trans-effect, and haplotype and editable regulatory region targets available for direct breeding are provided for genes dominated by the cis-effect.

The invention discloses application of a TaLBD30 protein and a coding gene thereof in regulating and controlling a wheat plant type. The invention belongs to the technical field of biology, and particularly relates to application of TaLBD30 protein and a coding gene thereof to regulation and control of wheat plant types. The protein is any one of the following proteins: A1) a protein with an amino acid sequence as shown in SEQ ID No: 1; a2) a protein which is obtained by substitution and / or deletion and / or addition of amino acid residues on the protein of A1), has 80% or more of identity with the protein of A1) and has the same function as the protein of A1); a3) a fusion protein obtained by connecting a protein tag to the N terminal or / and the C terminal of A1) or A2). TaLBD30 is a transcription factor with transcription inhibition activity, and compared with a wild type, the TaLBD30 gene overexpression shows that after TaLBD30 overexpression, the wheat plant height and ear length are remarkably reduced.

The invention is applicable to the technical field of bioengineering, and provides a coding gene for enhancing iron deficiency stress tolerance of plants and application. According to the soybean GmMYB14 transcription factor disclosed by the invention, the problem that the growth of soybeans is limited in an iron-deficient environment, particularly calcareous soil and soda saline alkali soil, is solved by excavating and applying the encoding gene (the nucleotide sequence is shown as SEQ ID No.1) of the soybean GmMYB14 transcription factor. Experiments prove that after the gene is over-expressed in arabidopsis thaliana and soybean, the tolerance of plants to iron deficiency stress can be remarkably enhanced. The invention provides a key gene resource for molecular breeding: the GmMYB14 effectively promotes the absorption and utilization of plants on iron by regulating and controlling the metabolic pathway of phenylpropane, not only can improve the planting adaptability of soybeans in low-iron soil, but also can improve the yield and quality of the soybeans, provides powerful technical support for enlarging the planting area of the soybeans in iron-deficient soil regions, and has a wide application prospect. The method is of great significance in relieving insufficient soybean productivity in China and improving the self-sufficiency rate.

The invention discloses a coumarin synthetic gene in black bamboo and application thereof, and belongs to the technical field of molecular biology. The invention provides transcription factors PnMYB4 and PnMYB4-1 for promoting synthesis of coumarin in black bamboos and application of the transcription factors PnMYB4 and PnMYB4-1. The PnMYB4 and the PnMYB4-1 belong to an MYB transcription factor family, the length of a complete coding frame of the PnMYB4 is 762 basic groups, and the coded protein contains 253 amino acids; the length of a complete coding frame of the PnMYB4-1 is 744 basic groups, and the coded protein contains 253 amino acids. The coumarin substances in arabidopsis thaliana, which are respectively overexpressed by PnMYB4 and PnMYB4-1, are obviously increased, and in addition, the expression levels of coumarin synthetic genes such as AtPAL1, At4CL1 and AtC4H in overexpressed plants are obviously increased.

The invention provides a cell specific transcription factor regulatory network analysis method and a visualization platform, and relates to the technical field of bioinformatics, the method comprises the following steps: constructing a gene regulatory network through a GRNBoost2-cisTarget-AUCell-Cell GRN workflow based on a transcription factor in combination with a motif database; screening a direct regulation relationship in combination with the database, and calculating an activity score of a regulator in each cell; based on activity scores and cell type annotation results, grouping the single cell data by using a unified manifold approximation and projection (UMAP) dimensionality reduction method and a Leiden clustering algorithm, and displaying the following results through an interactive visualization tool: a cell clustering UMAP graph, performing color marking according to cell types; a UMAP graph and a heat map of transcription factor regulator activity; according to the visual map of the gene regulation and control network, transcription factors and target genes are distinguished through node shapes, and regulation and control relations are marked through line weights and colors. According to the invention, an accurate regulation and control network can be provided.

The invention belongs to the technical field of plant gene engineering, and particularly relates to an OsNUC1 gene and application of a gene mutant constructed by the OsNUC1 gene to regulation and control of rice plant height, leaf length and panicle type. The regulation is to regulate the plant height and leaf growth of the rice and influence the shape of the ear part. Compared with the prior art, the OsNUC1 gene mutant has the following advantages: (1) the OsNUC1 is directionally edited by utilizing a CRISPR / Cas9 gene editing technology, an OsNUC1 gene mutant rice material is obtained, the biological phenotype of the OsNUC1 gene mutant rice material is investigated, the phenotype investigation on T1-generation seedlings of the OsNUC1 shows that the plant heights and the leaf lengths of one-week-old and two-week-old seedlings of the mutant are obviously higher than those of wild seedlings, the leaf widths have no obvious difference, and the OsNUC1 gene mutant rice material has a good application prospect; the result shows that the OsNUC1 can regulate the plant height and the leaf growth of the rice and influence the shape of the ear part. (2) it is proved that the nucleolin gene OsNUC1 is negatively regulated by a transcription factor OsARF7, and the phenotypes such as the plant height, the leaf length, the grain length and the grain width of the OsNUC1 / OsARF7 mutant are more obvious than those of the OsNUC1 mutant. The invention further proves the application of the OsNUC1 gene in regulation and control of rice plant height and leaf growth.

The invention belongs to the technical field of molecular biology, and particularly relates to a CeNF-YA3 gene, an expression vector and application of the CeNF-YA3 gene in vegetable fat regulation and control. The nucleotide sequence of the CeNF-YA3 gene is as shown in SEQ ID NO.1. After the CeNF-YA3 gene is over-expressed, the grease content of arabidopsis seeds and leaves can be remarkably increased; in addition, the invention also provides a series of vectors containing the CeNF-YA3 gene for subcellular localization, yeast hybridization and plant overexpression for the first time, and proves that the CeNF-YA3 protein has a transcriptional activation function, is localized in a cell nucleus and accords with the basic characteristics of transcription factors for the first time. Therefore, the technical scheme of the invention provides scientific guidance for the application of the CeNF-YA3 gene and the protein thereof.

The invention discloses application of an apple MdMYS1 gene in regulating and controlling the wax content of plant fruits and leaves, and belongs to the technical field of plant genetic engineering. According to the invention, the MdMYS1 gene with high expression quantity in apple varieties with more waxiness is separated, and the nucleotide sequence of the MdMYS1 gene is shown as SEQ ID NO. 1. Through subcellular localization, the transcription factor expressed by the MdMYS1 gene is found to be localized on a cell nucleus. Experimental results show that overexpression of the MdMYS1 gene can significantly increase the wax content of the apple fruits and leaves by promoting biosynthesis of wax, which indicates that the MdMYS1 gene plays a key role in regulation and control of the wax content of the apple fruits and leaves. The invention provides an efficient and rapid way for apple breeding, provides a gene material for improving apple quality, and has a wide application prospect in improving economic benefits and ecological benefits of apple planting.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a wheat transcription factor TaWRKY22 and a coding gene thereof in regulation and control of plant stripe rust resistance. The function of the wheat transcription factor TaWRKY22 is researched through molecular biology and genetics methods, it is found that knockout of the TaWRKY22 gene significantly improves the resistance of wheat to stripe rust, overexpression of the TaWRKY22 gene reduces the resistance of wheat to stripe rust, and then it is indicated that the wheat transcription factor TaWRKY22 plays a negative regulation role in wheat stripe rust resistance. Therefore, the TaWRKY22 gene is subjected to targeted modification through a gene editing technology, a new genetic material and a molecular breeding strategy can be provided for wheat stripe rust resistance breeding, and an important material and technical support are provided for breeding of wheat disease-resistant varieties.

The invention belongs to the technical field of agricultural biology, and particularly relates to a transcription factor StBSB with a regulation effect on steroid glycoalkaloid accumulation of potatoes and application of the transcription factor StBSB, the transcription factor StBSB is encoded by a potato gene Soltu.DM.05G001620, and the nucleotide sequence of the potato gene Soltu.DM.05G001620 is shown as SEQ ID NO.1. The invention further discloses a preparation method of the transcription factor StBSB. According to the application, a new insight is provided for understanding a transcriptional regulation mechanism of SGAs in solanaceae plants, and a key theoretical basis and genetic resources are provided for directionally improving the content of anti-nutritional compounds through genetic means.

The invention belongs to the technical field of biotechnology and genetic engineering, and particularly relates to application of recombinant RASA3 in preparation of a medicine for preventing and treating viral pneumonia. The recombinant RASA3 is used for promoting autophagy degradation of a transcription factor CEBP / beta protein and reducing the expression level of the transcription factor CEBP / beta protein, so that generation of inflammatory factors such as IL-6 is reduced, and inflammatory damage caused by excessive antiviral innate immune response in the virus infection process is improved. The invention provides a novel treatment strategy for inflammatory diseases caused by RNA viruses such as influenza and respiratory syncytial virus infection.

The invention belongs to the technical field of genetic engineering breeding, and particularly relates to application and a method of a transcription factor CaBTF3 for regulating capsorubin synthesis. According to the capsicum transcription factor CaBTF3 and the coding gene thereof provided by the invention, the biosynthesis of capsorubin can be influenced by adjusting the expression level of a key enzyme gene in a capsorubin synthesis route and regulating and controlling the development and quantity of fruit chloroplast, the expression of the CaBTF3 gene in capsicum fruits is inhibited, the accumulation amount of capsorubin in the capsicum fruits can be reduced, and the yield of capsorubin is increased. The color of the pepper fruit is further influenced. By enhancing the expression level of the CaBTF3 gene, the content of capsorubin in capsicum fruits can be increased, the gene is applied to plant genetic engineering breeding, a new effective way is provided for creating capsicum varieties with high capsorubin content, and the gene plays an important role in improving the economic benefits of the capsicum industry and popularizing and applying the capsorubin.

The invention provides a kiwi fruit transcription factor and application thereof in kiwi fruit canker, and belongs to the technical field of genetic engineering. The invention provides a transcription factor AcWRKY50, the nucleotide sequence of the transcription factor AcWRKY50 is shown as SEQ ID No.1, and the AcWRKY50 is positioned in a cell nucleus and has transcription activation capability. The AcWRKY50 disclosed by the invention can continuously respond to Psa infection, after Psa pathogenic bacteria infection, the expression of the AcWRKY50 gene is rapidly activated and continuously up-regulated, and after the AcWRKY50 gene is over-expressed, the resistance to Psa can be improved. Meanwhile, MeJA negatively regulates the resistance of the kiwi fruit to the Psa, and the kiwi fruit germplasm can be innovated based on the transcription factor disclosed by the invention.

The invention discloses an application of an NtWRKY59 gene in regulation and control of tobacco bacterial wilt resistance. A nucleotide sequence of the NtWRKY59 gene is shown as SEQ ID NO.1, and the NtWRKY59 gene is over-expressed in tobacco. The invention further discloses an application of the NtWRKY59 gene in regulation and control of tobacco bacterial wilt resistance. The NtWRKY59 gene provided by the invention not only enriches the genetic basis of tobacco bacterial wilt resistance as a brand new anti-disease gene resource, but also provides a new thought for developing a disease-resistant breeding strategy different from an upstream signal element as an action mechanism of a downstream transcription factor, and has good scientific research value and application prospect.

The invention belongs to the technical field of biosynthesis, and particularly relates to a method for regulating and controlling stevioside production through a UDPG synthesis route. According to the invention, a high-efficiency stevioside biosynthesis system is constructed through systematic engineering modification: in the aspect of chassis cell optimization, transcription factors (AtWRKY18, AtWRKY40 and AtMYC2) for regulating and controlling MEP pathway and key genes (dxs and / or dxr) of the MEP pathway are over-expressed, so that supply of terpene precursors is enhanced; and a heterologous MVA pathway (over-expression T7-atoB-mvaS-mvaE-mvK / mvKmut-pmK-mvaD-idi) with a modular design is introduced, so that sufficient supply of the terpenoid precursor is realized. Meanwhile, the intracellular concentration of the UDPG is increased by systematically enhancing a UDPG synthesis pathway (knocking out ugd, glgc, ushA, agp, deoA, tdk, udp, or thrA, and overexpressing galU, pgm, pyrE, pyrH, ugpA, or prs), so that the production capacity of the stevioside is remarkably improved.

The invention discloses application of a watermelon WRKY transcription factor ClWRKY60 in drought stress resistance, and belongs to the technical field of gene engineering. The invention discloses an application of a watermelon WRKY transcription factor ClWRKY60 in drought stress resistance. The nucleotide sequence of the watermelon WRKY transcription factor ClWRKY60 is as shown in SEQ ID NO. 1. The WRKY transcription factor ClWRKY60 is a positive regulation factor of watermelon responding to drought stress, the gene can provide gene resources for creation of drought-resistant new germplasm and breeding of special high-resistance new varieties, and meanwhile, the gene has very important scientific and production application significance for promoting resistance breeding, improving economic benefits of watermelons and the like.

The invention belongs to the technical field of biosynthesis, and particularly relates to a method for regulating and controlling stevioside production by improving the utilization rate of a carbon source. According to the invention, a high-efficiency stevioside biosynthesis system is constructed through systematic engineering modification: in the aspect of chassis cell optimization, transcription factors (AtWRKY18, AtWRKY40 and AtMYC2) for regulating and controlling MEP pathway and key genes (dxs and / or dxr) of the MEP pathway are over-expressed, so that supply of terpene precursors is enhanced; and a heterologous MVA pathway (over-expression T7-atoB-mvaS-mvaE-mvK / mvKmut-pmK-mvaD-idi) with a modular design is introduced, so that sufficient supply of the terpenoid precursor is realized. Meanwhile, by knocking out related genes and bypass metabolism genes (mgsA, iclR, menA or adhE) synthesized by organic acid, a glucose transport and utilization system (fnr, fadR, ppc, zwf or pgi is knocked out, and ACS, fbaA, tpiA, glf, galP or ptsG is overexpressed, so that the utilization rate of a carbon source is further improved, and the production capacity of stevioside is remarkably improved.

The invention discloses application of a transcription factor ZmbZIP27 in regulation and control of corn plant height and ear height, and belongs to the technical field of plant genetic engineering and corn molecular breeding. The invention reveals that the bZIP transcription factor family member ZmbZIP27 has an important biological function of regulating and controlling the corn plant height and lodging resistance for the first time. A ZmbZIP27 knockout vector is constructed through a CRISPR / Cas9 gene editing technology, and an obtained homozygous mutant strain shows remarkable plant type improvement characteristics compared with a wild type: the plant height is reduced by 8-12%, and the ear height is reduced by 15-22%. And a new gene target and a breeding strategy are provided for creating a new high-yield lodging-resistant corn variety suitable for mechanical harvesting.

The invention discloses a pennisetum purpureum CpERF37 transcription factor and cold-resistant application thereof, and relates to the technical field of plant genetic engineering. According to the invention, the Pennisetum purpureum-sourced ERF transcription factor CpERF37 is cloned and functionally identified for the first time, and heterologous expression verification in a model plant arabidopsis thaliana proves that the Pennisetum purpureum-sourced ERF transcription factor CpERF37 has a remarkable effect on improving the low-temperature tolerance of plants, and functional gene resources related to low-temperature stress response are enriched. The method not only has theoretical innovation, but also has a definite application prospect, and can provide effective gene resources and technical support for cold-resistant improvement of pasture and crops.

The invention discloses a high-flux and rapid chromatin immunoprecipitation sequencing method based on a HiBiT tag and a Tn5 enzyme, and belongs to the technical field of biology. According to the method, the high affinity characteristic of a HiBiT tag system is combined with a Tn5 enzyme in-situ library building technology, firstly, a DNA binding protein recombinant expression strain containing a HiBiT tag is constructed and subjected to induced expression, after formaldehyde crosslinking and cell lysis, an Anti-HiBiT antibody is used for rapid immunoprecipitation, and a Tn5 enzyme library is constructed; tn5 transposase is directly loaded on the magnetic beads combined with the protein and DNA fragment compound to complete fragmentation and linker connection, so that the construction of a next-generation sequencing library is quickly carried out, and the total consumed time from immunoprecipitation to the completion of library construction is less than or equal to 5 hours. Meanwhile, automatic treatment is realized through a micro experiment board (the reaction system is less than or equal to 200 microliters) and a liquid workstation, the experiment cost and the sample dosage are reduced, 48 transcription factors and 48 control samples can be synchronously treated, the efficiency is greatly improved, and an efficient tool is provided for microbial transcription regulation and control research.

The invention discloses a combination element of promoters of Dendrocalamus latiflorus flowering genes DlFT1 and DlFT2 and a transcription factor CO and application of the combination element. Nucleotide sequences of the promoters of the DlFT1 and the DlFT2 are shown as SEQ ID NO.1 and SEQ ID NO.2; the invention further discloses CO binding elements on a DlFT1 promoter and a DlFT2 promoter, and the sequences of the CO binding elements are respectively TGTGGATG and TGTGTACATG. According to the application of the CO-FT regulation and control module in the florescence conversion process of dendrocalamus latiflorus, specifically, DlCO1 can be directly combined with DlFT1 and DlFT2 promoters and plays a positive regulation and control role, which indicates that a CO-FT regulation and control way exists in florescence conversion of dendrocalamus latiflorus.

The invention relates to the technical field of plant genetic engineering, in particular to a transcription factor CsKNAT1 and application thereof in regulation and control of development of tea trees. The nucleotide sequence of the gene is as shown in SEQ ID NO: 1, and the sequence of protein coded by the gene is as shown in SEQ ID NO: 2. Research finds that overexpression of the CsKNAT1 gene can influence the form of plant leaves: the edges of the leaves are promoted to have notches (asymmetric leaves are formed, resulting in increase of sawteeth at the edges of the leaves); the formation of compound leaves can be promoted; leaves on plant stems can grow in a rosette form, and secondary stems are bolted in the middles of rosette leaves; the research also finds that the overexpression of the CsKNAT1 gene can obviously influence the flowering phase and the flower form of the plant; the delay of the flowering phase is promoted; overexpression of the CsKNAT1 gene can further influence development of floral organs and formation of siliques, part of arabidopsis plants have phenotypes that corolla becomes small, stamens are exposed, petals gradually extend and are zigzag in a wavy shape, the length of mature siliques is remarkably shortened by 20%-36.1%, and the phenomenon that the fruits are aborted occurs.

The invention provides a method for jointly deducing dynamic cell communication and a cell state transition rate. Relates to the technical field of biological information. The method comprises the following steps: extracting candidate ligands, candidate receptors and characteristic genes from space transcriptome data to be processed; the method comprises the following steps: screening nodes which have an interaction relationship with candidate ligands, candidate receptors and characteristic genes from a pre-constructed prior database, and constructing a multi-layer signal network of which the structure is ligand-receptor-transcription factor-target genes; establishing a gene regulation kinetic model according to a ligand-receptor action relationship, a receptor-transcription factor action relationship and a transcription factor-target gene action relationship in the multilayer signal network; iteratively optimizing parameters to be estimated in the gene regulation and control kinetic model; and obtaining the change rate of the expression quantity of the target gene in the receiving cell based on the potential time after iterative optimization and the parameters to be estimated after iterative optimization. And combined analysis of dynamic cell communication and cell differentiation tracks can be realized.

The banana transcription factor MabZIP2 disclosed by the invention is combined with a banana fruit MaLSF1 gene promoter to regulate up-regulated expression of the banana fruit MaLSF1 gene. The MabZIP2 expression is instantly silenced, the expression quantity of the MaLSF1 is reduced, the degradation of banana pulp starch is inhibited, and the content of total starch, amylose and amylopectin is increased, so that the sweet taste of banana fruits is favorably improved, and the quality of bananas is improved; the hardness of the banana pulp is improved, the softening of the banana after picking can be delayed, the ripening of the banana fruit after picking is delayed, and the storage period of the banana fruit is prolonged. The banana transcription factor MabZIP2 provides an important gene resource for improvement of banana fruit quality, provides a theoretical basis for research and development of a banana fresh-keeping technology and cultivation of new storage-resistant varieties, and also provides a new way for research and development of a fresh-keeping technology of starch storage type fruits such as bananas, molecular design breeding and cultivation of new storage-resistant banana varieties.

The invention discloses an application of an NtWRKY41 gene in regulation and control of tobacco bacterial wilt resistance. A nucleotide sequence of the NtWRKY41 gene is shown as SEQ ID NO.1, and the NtWRKY41 gene is over-expressed in tobacco. The invention further discloses an application of the NtWRKY41 gene in regulation and control of tobacco bacterial wilt resistance. The NtWRKY41 gene provided by the invention not only enriches the genetic basis of tobacco bacterial wilt resistance as a brand new anti-disease gene resource, but also provides a new thought for developing a disease-resistant breeding strategy different from an upstream signal element as an action mechanism of a downstream transcription factor, and has good scientific research value and application prospect.

The invention belongs to the technical field of plant genetic engineering, particularly relates to application of a transcription factor CitNAC71 in regulation and control of synthesis of citrus withered water and juice sac secondary walls, and discloses application of the transcription factor CitNAC71 or a biological material for regulating and controlling expression of the transcription factor CitNAC71 in regulation and control of synthesis of citrus withered water and / or secondary walls, and the amino acid sequence of the transcription factor CitNAC71 is shown as SEQ ID NO: 1. The invention discovers that the gene expression level of the coding gene CitNAC71 of the transcription factor CitNAC71 has a significant negative correlation trend with the juice cell withering degree and the cellulose and hemicellulose content, and experiments prove that the CitNAC71 is a conservative fruit hardness regulation factor. The invention lays a theoretical foundation for preventing and controlling the occurrence of dry water before and after the harvesting of the citrus, and provides a potential target for improving the texture of the citrus fruit by a genetic engineering technology.

The invention discloses application of a pear transcription factor PbrMADS1 to regulation and control of synthesis of stone cells and lignin as a component of the stone cells. A transcription factor PbrMADS1 gene separated from Dangshan pears belongs to SEP subfamily members, the nucleotide sequence of the transcription factor PbrMADS1 gene is shown as SEQ ID No.1, and the coded amino acid sequence of the transcription factor PbrMADS1 gene is shown as SEQ ID No.2 in a sequence table. The lignin content and the lignin synthetic gene expression quantity of the pear pulp callus with the PbrMADS1 being knocked out are remarkably reduced. Therefore, it is proved that the PbrMADS1 gene regulates formation of the pear fruit stone cells and the lignin component of the pear fruit stone cells. And the PbrMADS1 gene has the advantage of being capable of regulating and controlling a plurality of genes at the same time, so that a more efficient way is provided for molecular breeding. The discovery of the gene supplements and perfects a pear stone cell character transcriptional regulation mechanism, and provides a theoretical basis and gene resources for improving the pear fruit stone cell content.

The invention belongs to the technical field of molecular biology, and particularly relates to a DNA binding site of a fungus transcription factor AniJ and application. According to the invention, an echinocandin B gene cluster regulatory factor AniJ is taken as a research object, a ptH-SpRY-ACBE base editor and an sgRNA library covering an aniA promoter are utilized, 5-FOA screening is combined, and a binding motif RNGCTGAS of the AniJ in a core promoter region is excavated. The construction of an engineering strain containing a modified promoter (increasing the number of motifs) proves that the motifs can significantly enhance the expression of aniA and the yield of echinocandin B. And a novel molecular tool is provided for metabolic engineering of natural products of fungi.

The invention discloses a transcription factor OfMYB187 for regulating synthesis of beta-ionone of a sweet osmanthus fragrance component, an interaction protein OfCLpP of the transcription factor OfMYB187 as well as coding genes and application of the transcription factor OfMYB187 and the interaction protein OfCLpP, and belongs to the field of plant molecular biology. The amino acid sequence of the transcription factor OfMYB187 provided by the invention is as shown in SEQ ID NO. 2, and the amino acid sequence of the interaction protein OfCLpP of the transcription factor OfMYB187 is as shown in SEQ ID NO. 4. According to the application disclosed by the invention, a carrier constructed by promoters of OfMYB187, OfCLpP and OfCCD4 is jointly injected into a Ben's tobacco leaf, firefly and renilla determination is carried out, and a result shows that the OfMYB187 has an inhibition effect on a synthetase gene OfCCD4 of beta-ionone, but after interaction of the OfMYB187 and the OfCLpP, the OfMYB187 protein can be hydrolyzed, and the inhibition effect of the OfMYB187 protein on the OfCCD4 is relieved. The gene provided by the invention can directly or indirectly regulate and control the content of beta-ionone in osmanthus fragrans fragrance, and has a good application prospect.