11 results about "Betulinic acid" patented technology

The application belongs to the technical field of medicine, and discloses application of SERPINE1 protein or a coding gene thereof as a new target for hepatitis B virus treatment. The application first discloses that SERPINE1 promotes autophagy flux by directly combining PIKfyve protein, thereby significantly inhibiting HBV replication and HBsAg expression. Based on this, the application establishes a multi-dimensional target verification platform, and first discovers that a natural compound, betulinic acid, is a high-affinity agonist (KD=1.46 µM) of SERPINE1. In-vivo and in-vitro experiments prove that betulinic acid can effectively reduce the levels of HBsAg and HBV DNA, and has good safety. In addition, betulinic acid and entecavir show a synergistic antiviral effect (CI<1). The application provides a new target and treatment strategy for realizing functional cure of hepatitis B.

The invention provides a method for increasing the yield of betulinic acid produced from phellinus igniarius, and relates to the technical field of medicinal fungus culture. According to the method for increasing the yield of betulinic acid produced from phellinus igniarius, liquid strains of phellinus igniarius are inoculated into a PGO solid culture medium to be cultured, and the PGO solid culture medium is prepared from 20 g of ginseng powder, 15 ml of water and 2 g of soybean oil. According to the invention, the defects in the prior art are overcome, the phellinus igniarius is effectively promoted to produce betulinic acid by optimizing the formula of the culture medium, and a new direction is provided for the development of a phellinus igniarius culture product with high betulinic acid content.

The invention discloses a betulinic acid derivative as well as a synthesis method and application thereof. According to the betulinic acid derivative, the carboxyl position of betulinic acid C-28 is subjected to structural modification, and four halogenated aniline derivatives are obtained. Wherein the derivatives b, d and e show strong activity on staphylococcus aureus, the derivatives d and e also show strong activity on vancomycin-resistant staphylococcus aureus (VRSA), and the derivative o strongly inhibits streptococcus pneumoniae. Compared with a parent skeleton, the four derivatives show significantly enhanced antibacterial efficacy. Experimental data prove that the derivatives d and e and further derivatives thereof may provide a promising strategy for resisting drug-resistant gram-positive pathogens, and the derivative d can be further developed into a novel therapeutic agent for biofilm-mediated drug-resistant infection.

This invention provides a method for increasing the production of betulinic acid from *Sanghuang* bao's mushroom, relating to the field of medicinal fungal culture technology. The method involves inoculating a liquid strain of *Sanghuang* bao's mushroom into a PGO-added solid culture medium, wherein the PGO solid culture medium formulation is 20g ginseng powder + 15ml water + 2g soybean oil. This invention overcomes the shortcomings of existing technologies by effectively promoting the production of betulinic acid from *Sanghuang* bao's mushroom through optimized culture medium formulation, providing a new direction for the development of *Sanghuang* bao's mushroom culture products with high betulinic acid content.

The present application relates to the technical field of cosmetic raw materials, and particularly relates to a cosmetic composite raw material for removing acne spots and a preparation method thereof. The preparation method of the cosmetic composite raw material for removing acne spots comprises the following steps: preparation of betulinic acid-ostruthin co-assembled nanoparticles, double modification of mannose, preparation of retinol slow-release particles, and preparation of the cosmetic composite raw material. The present application realizes efficient acne spot removal through the synergy of multiple components. The core components, such as betulinic acid, have multiple anti-inflammatory and antibacterial effects, ostruthin inhibits melanin production, glutathione lightens old pigments, retinol and salicylic acid accelerate keratin metabolism, the co-assembly of betulinic acid-ostruthin nanoparticles improves bioavailability, modified mannose / epsilon-polylysine layer-by-layer embedding realizes retinol slow-release synergism, the synergy among the components enhances the effects of spot lightening and anti-inflammation, and the modified mannose can also repair the skin barrier, so that the effect of targeted treatment and barrier repair for removing acne spots is finally realized.

The invention relates to the technical field of cosmetic raw materials, in particular to a cosmetic composite raw material for removing acne spots and a preparation method of the cosmetic composite raw material. The invention relates to a preparation method of a cosmetic composite raw material for removing acne spots. The preparation method comprises the following steps: preparing betulinic acid-cnidium lactone co-assembled nanoparticles, performing dual modification on mannan, preparing retinol sustained-release particles and preparing the cosmetic composite raw material. According to the invention, efficient acne spot removal is realized through cooperation of multiple components, the core component betulinic acid and the like have multiple anti-inflammatory and antibacterial effects, the cnidium lactone and the like inhibit melanogenesis, glutathione reduces old pigment, retinol and salicylic acid accelerate keratin metabolism, and bioavailability is improved through betulinic acid-cnidium lactone co-assembly nanocrystallization; modified mannan / epsilon-polylysine is embedded layer by layer to realize slow release and synergism of retinol, the components synergistically enhance the spot fading and anti-inflammatory effects, and modified mannan can repair the skin barrier to finally realize the acne spot removing effects of targeted therapy and barrier repair.

The application provides application of white birch acid in calcite inhibition. The molecular structure of the white birch acid contains functional groups such as hydroxyl, carboxyl and carbon six-membered ring, which are hydrophilic groups and solidophilic groups, have strong adsorption on calcite, make the white birch acid easy to be adsorbed with the calcite, and are not easy to be adsorbed with the fluorite ore. The excellent selectivity expands the floatability difference between the calcite and the fluorite ore, so that the selectivity of the fluorite ore flotation method based on the white birch acid is excellent, and the flotation effect is good. Compared with the existing inhibitors such as water glass, the white birch acid has stronger selectivity when used as the fluorite ore flotation inhibitor, and can obtain better flotation effect under the condition of smaller dosage, and the white birch acid is non-toxic and has small environmental pollution.

The betulinic acid extraction device comprises a containing box, a stirring frame and a separation barrel, a containing cavity is formed in the containing box, and a containing opening is formed in the upper side of the containing cavity; the stirring frame is arranged in the containing cavity, a vertically-arranged containing cavity is formed in the stirring frame, and the containing cavity corresponds to the containing opening; a plurality of through holes are formed in the surface of the separation barrel and used for separating solid and liquid inside and outside the separation barrel. According to the betulinic acid extraction device, when the stirring frame rotates, liquid on the outer side of the containing cavity is driven to move, so that the liquid in the separation barrel is also driven, solids and the liquid in the separation barrel are fully mixed, and the betulinic acid extraction efficiency is improved; and the other separation barrel filled with the unextracted raw materials is loaded into the accommodating box for next extraction, so that the material changing process is fast, the extracting solution only contains a small amount of fixed powder, the coarse filtering procedure is reduced, and the process difficulty is greatly reduced.

The invention discloses a method for increasing the yield of betulinic acid synthesized by saccharomyces cerevisiae, and belongs to the field of bioengineering and metabolic engineering. According to the invention, a synthetic route of betulinic acid is constructed in saccharomyces cerevisiae. Through overexpression of key genes such as tHMG1, IDI, ERG20, ERG9 and ERG1, construction and optimization of an IUP pathway and adoption of a multi-copy integration and promoter optimization strategy, the expression level of RoCYP01 is enhanced, the RoCYP01 gene is further subjected to rational design and directional modification, the substrate specificity and catalytic efficiency of the RoCYP01 gene are optimized, and the yield of the target product betulinic acid reaches 118.65 mg / L. In addition, by increasing supply of cofactors, conditions in the fermentation process are optimized, and the final yield of betulinic acid is successfully increased to 425.2 mg / L by adopting a continuous feeding culture strategy.

A COMPOSITION FOR TREATING KERATIN MATERIAL AND METHODS THEREOF The present disclosure provides a composition (A) comprising: a. oleanolic acid, its salts, solvates, or their mixtures thereof; b. ursolic acid, its salts, solvates, or their mixtures thereof; c. betulinic acid, its salts, solvates, or their mixtures thereof; and d. micromeric acid, its salts, solvates, or their mixtures thereof. The present disclosure further provides a rosemary extract and a composition (B). The present disclosure also provides a process for preparing the composition (A), the extract and methods thereof.

This invention discloses a betulinic acid derivative, its preparation method, and its uses. Using betulinic acid, a natural lupin-type pentacyclic triterpenoid, as the parent compound, a series of betulinic acid derivatives based on C-2 and C-3 position structural modifications were synthesized sequentially via oxidation and Claisen condensation reactions. These betulinic acid derivatives exhibit excellent α-glucosidase inhibitory activity. Their chemical structures differ significantly from existing clinical drugs, and their parent compounds are derived from natural products. This invention holds promise for overcoming the gastrointestinal adverse reactions and other drawbacks of traditional α-glucosidase inhibitors, providing new drug candidates for diabetes treatment. Furthermore, the raw materials for this invention are relatively widely available, facilitating subsequent scale-up production and process optimization, and possessing the potential for further development into clinical drug candidates.