148 results about "Ursolic acid" patented technology

The invention discloses a beta-glucuronidase inhibitor composition as well as a preparation method and application thereof. The composition is prepared from the following substances: ursolic acid, procyanidine, icariin, curcumin, epigallocatechin gallate and glycyrrhetinic acid. The beta-glucuronidase inhibitor composition has a broad-spectrum and good inhibition effect, the preparation method is simple, large-scale production is easy, and the inhibitor with a natural source is higher in use compliance, lower in toxic and side effects and more suitable for developing intestinal health products and has an application prospect.

The invention discloses application of ursolic acid in resisting fish ichthyophthiriasis. The ursolic acid has a remarkable killing effect on ichthyophthirius multifilis in an in-vitro stage, and the 4-hour half effective insecticidal concentration (4h-EC50) of the ursolic acid on a grazing body is 0.3774 mg / L; and the 22-hour half effective insecticidal concentration (22h-EC 50) on the capsule is 4.931 mg / L. An acute toxicity test shows that the 96-hour median lethal concentration (96h-LC50) of ursolic acid to goldfish is 9.358 mg / L, the therapeutic index (TI = 96h-LC50 / 4h-EC50) is up to 24.8, and the safety of ursolic acid is remarkably superior to that of a traditional medicine. Ursolic acid used in the invention is a plant-derived active component, has the characteristics of high environmental compatibility and low biological accumulation, meets the development requirements of green fishery, and has an industrialization basis for being developed into eco-friendly aquatic products.

The present application provides ursolic acid diethanolamine salt and ursolic acid morpholine salt. It also provides a composition comprising the salt, and a method of using the salt.

The invention provides diethanolamine and morpholine salts of ursolic acid. Compositions containing the salts, and methods of using the salts are also provided.

The present invention discloses a method for preparing Lactobacillus casei and a preparation thereof. The Lactobacillus casei was deposited in the China General Microbiological Culture Collection Center on July 6, 2022, with a deposit number of CGMCC No. 25194. The preparation method of the double-layer emulsion probiotic preparation comprises the following steps: (1) vortexing Lactobacillus casei and a probiotic protective agent solution to uniformly disperse them to form an inner water phase W1; (2) dissolving ursolic acid in oil to form an oil phase O, and mixing the oil phase O with the inner water phase W1 by high-speed shearing to obtain an oil-in-water type colostrum W1 / O; (3) using a suspension of colloidal particles as an external water phase W2, adding the external water phase W2 to the colostrum W1 / O, and mixing by medium-speed shearing to obtain a double-layer emulsion probiotic preparation with a W1 / O / W2 type double emulsion structure.

The application belongs to the technical field of biological medicine, and particularly relates to preparation and application of a self-assembled nanocomposite based on a CRISPR / Cas9 system and ursolic acid. The nanocomposite takes UA self-assembled nanodrug UA NPs as a core, and is co-assembled with low-molecular-weight protamine LMWP having good cell penetration capacity and a CRISPR / Cas9 plasmid pTIM3 for knocking down TIM3 to form a nanocomposite ULP NPs. The nanocomposite prepared in the application can be enriched at a tumor site through EPR effect, and can reach the effect of enhancing liver cancer immunotherapy by knocking down the inhibitory immune checkpoint TIM3. The regulation characteristics and molecular mechanism of the UA NPs as a self-assembled general template for delivering the CRISPR / Cas9 system to inhibit the immune checkpoint TIM3 are disclosed, and the application has a wide application prospect in liver cancer immunotherapy.

This invention discloses a Zhishu Yangwei tablet for chronic gastritis and its granule dry-compression tableting process, relating to the field of traditional Chinese medicine preparation technology. The Yangwei tablet is made from the following raw materials in parts by weight: Codonopsis pilosula 35-45 parts, stir-fried Atractylodes macrocephala 18-22 parts, Poria cocos 18-22 parts, Glycyrrhiza uralensis 8-12 parts, Amomum villosum 18-22 parts, Lindera strychnifolia 18-22 parts, Salvia miltiorrhiza 45-55 parts, roasted Aucklandia lappa 18-22 parts, Paeonia lactiflora 18-22 parts, stir-fried Citrus aurantium 18-22 parts, Magnolia officinalis 28-32 parts, Lilium brownii 35-45 parts, and stir-fried chicken gizzard lining 18- 22 parts, dried plum 45-55 parts, Coptis chinensis 8-12 parts, hawthorn 35-45 parts, magnesium stearate 5-7 parts, sucrose 28-32 parts; This invention uses low-temperature dynamic extraction technology to replace the traditional high-temperature decoction process, effectively reducing the decomposition loss of heat-sensitive active ingredients such as tanshinone IIA and ursolic acid, and improving the bioavailability of the drug. It also uses membrane concentration and spray drying technology to replace the traditional atmospheric pressure concentration and oven drying process, shortening the production cycle, reducing energy consumption, and avoiding the degradation of active ingredients caused by prolonged heating of materials.

The invention discloses application of geum aleppicum total triterpenes in treatment of hypertension and a preparation method of the geum aleppicum total triterpenes, the geum aleppicum total triterpenes are obtained by step-by-step extraction and purification of geum aleppicum, and the geum aleppicum total triterpenes comprise bitter raspberry sweet F1, 2-hydroxyl oleanolic acid, 2-hydroxyl ursolic acid, 20beta, 20beta, 20beta, 20beta, 20beta, 20beta, 20beta, 20beta, 20beta The traditional Chinese medicine composition is prepared from the following raw materials in percentage by weight: 28-epoxy-28-hydroxytaxasan-3 beta-ol (taraxane triterpenes), ursolic acid, maslinic acid, rosa multiflora acid and tormentic acid. According to the present invention, the geum aleppicum total triterpenes are extracted and purified from the geum aleppicum step by step, and the geum aleppicum total triterpenes can significantly increase the total cross-sectional area of the vascular bed of the ischemic tissue or organ, such that the low blood pressure can meet the blood perfusion for maintaining the life activity so as to achieve the blood pressure lowering purpose; the compound can be widely applied to the field of hypertension prevention and treatment medicines.

The invention relates to the technical field of natural product extraction, in particular to an ultrasonic-assisted extraction method of a dogwood extract containing ursolic acid. The ultrasonic-assisted extraction method comprises the following steps: step 1, cleaning dried dogwood, removing stems and leaves, drying and grinding to obtain dried dogwood powder; step 2, adding the dried dogwood fruit powder into a solvent, carrying out ultrasonic treatment, centrifuging, and taking supernate, so as to obtain a dogwood extract containing ursolic acid; the solvent comprises ethanol and water. According to the method disclosed by the invention, the extraction rate of the dogwood extract containing ursolic acid can be obviously improved, the cost is low, large-scale application in the industry is facilitated, and important technical support and theoretical basis are provided for high-value development and utilization of dogwood resources.

The present invention discloses a method for screening effective substances of traditional Chinese medicine based on a 6-dimensional spider web model, belonging to the field of medical technology. Based on the results of UHPLC analysis, the present invention uses danshensu, chlorogenic acid, procyanidin B2, epicatechin, hyperoside, isoquercetin, rosmarinic acid, lithospermic acid, salvianolic acid B, salvianolic acid A, dihydrotanshinone I, tanshinone I, cryptotanshinone, tanshinone IIA, oleanolic acid, and ursolic acid as the "specific" candidate effective ingredients of the Danshen-Hawthorn medicinal pair. Based on the data of the candidate effective ingredients in six dimensions, namely, effectiveness dimension, compatibility environment dimension, transmission and traceability dimension, content measurability dimension, network pharmacology degree value dimension, and molecular docking target binding activity dimension, a "spider web" model is constructed to obtain the key effective substances of the Danshen-Hawthorn medicinal pair. The present invention provides theoretical support for revealing the compatibility theory of the Danshen-Hawthorn medicinal pair, formulating its quality evaluation standards, and clinical application of the Danshen-Hawthorn medicinal pair.

The present invention belongs to the technical field of biological medicine, and discloses a ceramide compound shown in Chemical Formula I: #imgabs0# wherein, R 1 is selected from the residues after condensation of sialic acid, chelidamic acid monohydrate, 2,5-furandicarboxylic acid, quinic acid, chlorogenic acid, ectoine, nicotinic acid, shikimic acid, glucuronic acid, salicylic acid, carnosic acid, dihydrooat acylanthranilic acid, abietic acid, ursolic acid, tranexamic acid, syringic acid, gentisic acid, vanillic acid, 5-hydroxyisovanillic acid, orotic acid, 5-aminoorotic acid, 3,5-dihydroxy-4-methylbenzoic acid, glycyrrhizic acid or gallic acid, R 2 is selected from one of the following structures: -C 15 H 29 、-C 15 H 31 、-C 15 H 27 、-CHOHC 14 H 27 、-CHOHC 14 H 29 . The present invention also discloses a preparation method and application of the ceramide compound. The present invention introduces a molecular fragment with physiological activity of a functional carboxylic acid into ceramide, which will enhance the original efficacy of this type of molecule and also improve the physicochemical properties of the ceramide compound.

The invention provides a method for efficiently extracting ursolic acid from loquat leaves and application of the ursolic acid. Comprising the following steps: S1, taking fresh folium eriobotryae, cleaning, and drying at 45 DEG C until the water content is 1t; after 8%, crushing, sieving with a 60-mesh sieve, and sterilizing with microwaves to obtain sterilized loquat leaf powder; s2, mixing sterilized folium eriobotryae powder, a yeast extract, MgSO4. 7H2O and water, adjusting the pH value to 5.0, taking the mixture as a fermentation culture medium, synchronously inoculating a 5% saccharomyces cerevisiae seed solution and a 10% trametes versicolor seed solution, and performing shake culture at the temperature of 30 DEG C and the speed of 150 rpm for 36-48 hours; s3, adding a surfactant, uniformly stirring, and performing ultrasonic extraction to obtain an extracting solution; and S4, centrifuging the extracting solution at 4000-5000 rpm for 10-15 minutes, taking supernate, filtering with a filtering membrane, concentrating and freeze-drying. According to the method, double-bacterium synchronous synergistic fermentation and a high-dose surfactant-assisted ultrasonic extraction technology are combined, so that a strong synergistic effect is formed, and the problems of low extraction rate, poor purity, high energy consumption, large solvent dosage and the like in a traditional loquat leaf ursolic acid extraction method are fundamentally solved.

The invention belongs to the technical field of chemical pharmacy, and particularly relates to ursolic acid nanoparticles and a preparation method and application thereof. The nano-particles containing ursolic acid are prepared by taking methoxy polyethylene glycol terminal modified poly (lactic-co-glycolic acid) as a carrier material and ursolic acid as a drug, and the nano-particles can be directly administered by bypassing a blood-brain barrier to treat neurodegenerative diseases; the problems that the liver first-pass effect of the medicine is caused by intravenous injection and oral administration and the intracerebral delivery efficiency is reduced due to medicine interception after the medicine passes through a circulatory system are avoided, and the nano-particles can effectively protect the entrapped medicine from being degraded by hydrolase.

The present application relates to ursolic acid with improved solubility and dispersibility, a method for producing same, and a method for improving the solubility of ursolic acid. In the present application, a complex of ursolic acid, an emulsifier, and a nonionic surfactant is formed and made into microparticles, thereby improving the solubility and dispersibility of poorly soluble ursolic acid and facilitating the utilization of ursolic acid as a nutritional material.

The invention relates to a pharmaceutical composition of ursolic acid and a multi-target tyrosine kinase receptor inhibitor, which has a synergistic effect in the treatment of cancer, especially liver cancer.

A pharmaceutical combination of ursolic acid and a multi-target tyrosine kinase receptor inhibitor, which have a synergistic effect in the treatment of cancer, especially in the treatment of liver cancer.

The invention discloses a composite plant extract helpful for enhancing immunity and a preparation method and application thereof, the composite plant extract comprises the following raw materials by weight: 30-40 parts of a Poria cocos extract, 20-30 parts of a Radix Codonopsis extract, 20-30 parts of a honeysuckle extract, 5-10 parts of a Cornus officinalis extract, and 5-10 parts of a mulberry leaf extract. The composite plant extract is prepared by compounding a poria cocos extract, a codonopsis pilosula extract, a honeysuckle flower extract, a dogwood extract and a mulberry leaf extract, wherein chlorogenic acid in the honeysuckle flower extract can enhance the phagocytic ability of cells; a main component codonopsis pilosula polysaccharide in the codonopsis pilosula extract can improve indexes and responses of immune organs such as spleen, thymus, liver and intestinal lymph nodes, active substances such as polysaccharide, ursolic acid and iridoid glycoside in the dogwood extract also have a relatively good immunoregulation effect, and under the synergistic effect of the raw materials, the immunity of the human body is improved. And the composite plant extract can play a better role in enhancing immunity.

The invention discloses a leonurine hydrochloride and ursolic acid self-assembled nano preparation and application thereof in preparation of a medicine for treating acute lung injury. The invention provides a leonurine and ursolic acid self-assembled nano-preparation. The leonurine and ursolic acid self-assembled nano-preparation is obtained by self-assembling leonurine pharmaceutically acceptable salts and ursolic acid, wherein the amounts of the leonurine pharmaceutically acceptable salts and the ursolic acid are equal. The self-assembled nanoparticles have an obvious anti-lung inflammation effect, and the anti-inflammatory effect is obviously superior to that of a composition of the leonurine pharmaceutically acceptable salt and ursolic acid with the same dosage. Therefore, the leonurine and ursolic acid self-assembled nano preparation provided by the invention has the prospect of being developed into the medicine for treating the acute lung injury.

The invention provides application of ursolic acid in improving transient expression efficiency of tobacco leaves, and belongs to the technical field of bioengineering. The invention creatively finds that when ursolic acid is applied to a tobacco transient expression system, the transient expression efficiency of tobacco leaves can be remarkably improved. Experiments prove that after a proper amount of ursolic acid is added for treatment, the yield of exogenous protein in the tobacco leaves is greatly increased, so that powerful support is provided for large-scale production and scientific research of high-value biological products; ursolic acid can be used as a universal transient expression enhancement means, and is suitable for various different biotechnology application scenes. As a natural product existing in nature, ursolic acid has the characteristics of good biocompatibility and low toxicity, and does not cause harm to experimental subjects. Meanwhile, the synthesis cost is relatively low, the material is easy to obtain, and the principle of sustainable development is met. Therefore, ursolic acid can be predicted to have a wide application prospect in the field of transient expression of tobacco.

The present patent application describes a nutraceutical composition comprising ursolic acid, tomatine and polyphenols from grape pomace. Said composition resulted to be useful in counteracting muscle atrophy associated with neuropathic pain.

The invention belongs to the field of biological medicine, and particularly relates to a Bama pig model for preclinical research of a weight-reducing medicine COST and application of the Bama pig model. Healthy Bama pigs are firstly adaptively fed with common feed and then fed with high-fat feed for molding, and obesity-related indexes of the Bama pigs are detected in a fixed period of time every week; the high-fat feed comprises common feed, beef tallow, margarine, cane sugar, casein, laboratory animal premix, microcrystalline cellulose, calcium bicarbonate, gypenosides, mint leaves, maslinic acid, ursolic acid, bighead atractylodes rhizome and nutmeg. According to the invention, the Bama pig model with stable phenotype can be rapidly constructed, a reliable model is provided for preclinical research of new drugs for treating obesity, and guarantee is provided for research of obesity mechanisms and screening, development and mechanism research of the new drugs for treating obesity.

The present invention relates to the field of feed additive technology, and in particular to a feed additive composition and application thereof. The present invention provides a feed additive composition, wherein the composition comprises ursolic acid and flavonoid glycosides in a mass ratio of (0.5-5): 1. The present invention finds that ursolic acid and flavonoid glycosides can play a synergistic effect in reducing the mortality rate of livestock and poultry. In the case of equal dietary addition, the effect of ursolic acid and flavonoid glycosides used in combination is significantly higher than that of ursolic acid and flavonoid glycosides added separately. The feed additive composition provided by the present invention can effectively reduce the mortality rate of livestock and poultry, improve the growth performance of livestock and poultry, promote their weight gain, increase average daily feed intake and average daily weight gain, reduce feed-to-weight ratio to a certain extent, and is of great significance for the development of feed additives for replacing antibiotics.

The invention belongs to the technical field of biology and new medicine, and discloses a preparation method of acoustic resonance driven glycyrrhizic acid nano-composite responsive gel, glycyrrhizic acid is used as a drug carrying system, berberine, ursolic acid, baicalein, luteolinin, curcumin, baicalin or ginsenoside Rh2 is used as a water-insoluble natural small molecule drug, and the acoustic resonance driven glycyrrhizic acid nano-composite responsive gel is prepared. Glycyrrhizic acid is an amphiphilic compound composed of one molecule of glycyrrhetinic acid and two molecules of glucuronic acid, a hydrophobic cavity can be formed in a water environment to wrap the water-insoluble natural small molecule medicine, a glycyrrhizic acid nano-composite solution is formed, and meanwhile the solubility of the water-insoluble natural small molecule medicine such as baicalein is improved.

The present application relates to the field of animal breeding technology, in particular to the application of ursolic acid in the frozen semen preservation of sheep. The present application applies ursolic acid in the frozen semen preservation of sheep. The addition of 0.5-2.0 micromole / L ursolic acid in the frozen diluent can improve the quality of frozen semen preservation of sheep and goats, and can improve the sperm motility and vitality after thawing, which helps the thawed sperm to pass through the vagina, uterus and enter the oviduct to complete fertilization, improves the insemination success rate of frozen sperm, and provides technical support for strengthening the protection of local sheep breed resources.

This invention provides a highly efficient extraction method for ursolic acid from loquat leaves and its application. The method includes the following steps: S1. Fresh loquat leaves are taken, washed clean, dried at 45℃ until the moisture content is <8%, pulverized, passed through a 60-mesh sieve, and microwave-sterilized to obtain sterilized loquat leaf powder; S2. The sterilized loquat leaf powder, yeast extract, MgSO4·7H2O, and water are mixed, and the pH is adjusted to 5.0 as a fermentation medium. Simultaneously, 5% *Saccharomyces cerevisiae* seed culture and 10% *Trametes versicolor* seed culture are inoculated and cultured at 30℃ and 150 rpm for 36-48 h with shaking; S3. A surfactant is added, stirred evenly, and ultrasonically extracted to obtain the extract; S4. The extract is centrifuged at 4000-5000 rpm for 10-15 min, the supernatant is filtered through a membrane, concentrated, and freeze-dried. This invention combines simultaneous synergistic fermentation by two bacteria with high-dose surfactant-assisted ultrasonic extraction technology, resulting in a powerful synergistic effect. This fundamentally solves the problems of low extraction rate, poor purity, high energy consumption, and large solvent consumption in traditional loquat leaf ursolic acid extraction methods.

This invention provides an acid-resistant nutrient-carrying nanomicelles and its preparation method, comprising: (1) dissolving ursolic acid, 1-hydroxybenzotriazole, and 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride in a solvent and stirring to obtain an activated ester; (2) reacting an amine-containing cationic polymer with the activated ester obtained in step (1) to obtain an amphiphilic cationic polymer; (3) mixing the amphiphilic cationic polymer with a lipid-soluble active substance in a good solvent, removing the good solvent by rotary reflux in the dark to obtain nutrient-carrying nanomicelles; and (4) mixing the polyanionic polymer solution with the nutrient-carrying nanomicelles in water to obtain acid-resistant nutrient-carrying nanomicelles. The nanomicelles prepared by this invention can withstand gastric digestion and gel and aggregate in the enteritis area of ​​mice by prolonging the intestinal retention time.

The invention discloses a Lactobacillus paracasei strain capable of converting licorice and enhancing the licorice to relieve lipid accumulation, oxidative damage and inflammatory response, and belongs to the technical field of microorganisms and the technical field of medicines. The lactobacillus paracasei CCFM1361 can convert glycyrrhizic acid, increase the content of glycyrrhetinic acid, glycyrrhetinic acid 3-O-glucuronide, ursolic acid and other substances, and increase the efficacy of the licorice extract, which is specifically embodied in (1) inhibiting the weight gain of high fat diet mice; (2) reducing liver lipid accumulation and lesion caused by the liver lipid accumulation; (3) the anti-oxidation level of the liver of the high-fat diet mouse is improved; (4) the inflammatory reaction caused by lipid accumulation is improved; and (4) reducing the inflammation level of the organism by activating an NF-kappa B p65 related pathway. Therefore, the lactobacillus paracasei CCFM1361 and glycyrrhizic acid have huge application prospects in production of products with the efficacy of resolving lipid accumulation and oxidative damage and inflammatory reaction caused by lipid accumulation.

The invention discloses an application of acetyl ursolic acid in promoting the growth of burkholderia. Eight Burkholderia strains are separated from soil, the separated eight Burkholderia strains are cultured in a nitrogen-free culture medium added with 50 mg / L of acetyl ursolic acid, and the result shows that the biomass of Burkholderia can be increased by 40%-95% compared with that of a control group without acetyl ursolic acid within 24 h through acetyl ursolic acid, the Burkholderia strain has a remarkable growth promoting effect on Burkholderia, and the Burkholderia strain has a good application prospect. The core functions such as nitrogen fixation are not influenced. The concentration gradient experiment further determines that the effective concentration range of the acetyl ursolic acid on the growth promotion of the burkholderia is 5-100 mg / L, and the optimal concentration of the acetyl ursolic acid is 25-75 mg / L. Acetyl ursolic acid is used as a specific growth promoting agent of burkholderia, not only are the problems of specificity, safety and stability in the prior art solved, but also the synergistic effect of promoting growth and improving crop quality is realized.

The invention relates to an extract from Buddleja saligna, or a bioactive mixture consisting essentially of oleanolic acid and ursolic acid isolated from Buddleja saligna, for use in preventing and / or treating skin cancer, more specifically melanoma, and for reducing skin damage resulting from UV radiation. The invention also relates to uses and methods of treating skin cancer and reducing skin damage resulting from UV radiation using the extracts and / or bioactive mixture described. The invention further relates to an anticancer composition comprising the extracts and / or bioactive mixture, that inhibits angiogenesis and / or proliferation of cells associated with melanoma, as well as a sunscreen composition comprising the extracts and / or bioactive mixture.