63 results about "Ursolic acid" patented technology

The invention discloses application of ursolic acid in resisting fish ichthyophthiriasis. The ursolic acid has a remarkable killing effect on ichthyophthirius multifilis in an in-vitro stage, and the 4-hour half effective insecticidal concentration (4h-EC50) of the ursolic acid on a grazing body is 0.3774 mg / L; and the 22-hour half effective insecticidal concentration (22h-EC 50) on the capsule is 4.931 mg / L. An acute toxicity test shows that the 96-hour median lethal concentration (96h-LC50) of ursolic acid to goldfish is 9.358 mg / L, the therapeutic index (TI = 96h-LC50 / 4h-EC50) is up to 24.8, and the safety of ursolic acid is remarkably superior to that of a traditional medicine. Ursolic acid used in the invention is a plant-derived active component, has the characteristics of high environmental compatibility and low biological accumulation, meets the development requirements of green fishery, and has an industrialization basis for being developed into eco-friendly aquatic products.

This invention discloses a Zhishu Yangwei tablet for chronic gastritis and its granule dry-compression tableting process, relating to the field of traditional Chinese medicine preparation technology. The Yangwei tablet is made from the following raw materials in parts by weight: Codonopsis pilosula 35-45 parts, stir-fried Atractylodes macrocephala 18-22 parts, Poria cocos 18-22 parts, Glycyrrhiza uralensis 8-12 parts, Amomum villosum 18-22 parts, Lindera strychnifolia 18-22 parts, Salvia miltiorrhiza 45-55 parts, roasted Aucklandia lappa 18-22 parts, Paeonia lactiflora 18-22 parts, stir-fried Citrus aurantium 18-22 parts, Magnolia officinalis 28-32 parts, Lilium brownii 35-45 parts, and stir-fried chicken gizzard lining 18- 22 parts, dried plum 45-55 parts, Coptis chinensis 8-12 parts, hawthorn 35-45 parts, magnesium stearate 5-7 parts, sucrose 28-32 parts; This invention uses low-temperature dynamic extraction technology to replace the traditional high-temperature decoction process, effectively reducing the decomposition loss of heat-sensitive active ingredients such as tanshinone IIA and ursolic acid, and improving the bioavailability of the drug. It also uses membrane concentration and spray drying technology to replace the traditional atmospheric pressure concentration and oven drying process, shortening the production cycle, reducing energy consumption, and avoiding the degradation of active ingredients caused by prolonged heating of materials.

The invention discloses application of geum aleppicum total triterpenes in treatment of hypertension and a preparation method of the geum aleppicum total triterpenes, the geum aleppicum total triterpenes are obtained by step-by-step extraction and purification of geum aleppicum, and the geum aleppicum total triterpenes comprise bitter raspberry sweet F1, 2-hydroxyl oleanolic acid, 2-hydroxyl ursolic acid, 20beta, 20beta, 20beta, 20beta, 20beta, 20beta, 20beta, 20beta, 20beta The traditional Chinese medicine composition is prepared from the following raw materials in percentage by weight: 28-epoxy-28-hydroxytaxasan-3 beta-ol (taraxane triterpenes), ursolic acid, maslinic acid, rosa multiflora acid and tormentic acid. According to the present invention, the geum aleppicum total triterpenes are extracted and purified from the geum aleppicum step by step, and the geum aleppicum total triterpenes can significantly increase the total cross-sectional area of the vascular bed of the ischemic tissue or organ, such that the low blood pressure can meet the blood perfusion for maintaining the life activity so as to achieve the blood pressure lowering purpose; the compound can be widely applied to the field of hypertension prevention and treatment medicines.

The invention relates to the technical field of natural product extraction, in particular to an ultrasonic-assisted extraction method of a dogwood extract containing ursolic acid. The ultrasonic-assisted extraction method comprises the following steps: step 1, cleaning dried dogwood, removing stems and leaves, drying and grinding to obtain dried dogwood powder; step 2, adding the dried dogwood fruit powder into a solvent, carrying out ultrasonic treatment, centrifuging, and taking supernate, so as to obtain a dogwood extract containing ursolic acid; the solvent comprises ethanol and water. According to the method disclosed by the invention, the extraction rate of the dogwood extract containing ursolic acid can be obviously improved, the cost is low, large-scale application in the industry is facilitated, and important technical support and theoretical basis are provided for high-value development and utilization of dogwood resources.

The invention provides a method for efficiently extracting ursolic acid from loquat leaves and application of the ursolic acid. Comprising the following steps: S1, taking fresh folium eriobotryae, cleaning, and drying at 45 DEG C until the water content is 1t; after 8%, crushing, sieving with a 60-mesh sieve, and sterilizing with microwaves to obtain sterilized loquat leaf powder; s2, mixing sterilized folium eriobotryae powder, a yeast extract, MgSO4. 7H2O and water, adjusting the pH value to 5.0, taking the mixture as a fermentation culture medium, synchronously inoculating a 5% saccharomyces cerevisiae seed solution and a 10% trametes versicolor seed solution, and performing shake culture at the temperature of 30 DEG C and the speed of 150 rpm for 36-48 hours; s3, adding a surfactant, uniformly stirring, and performing ultrasonic extraction to obtain an extracting solution; and S4, centrifuging the extracting solution at 4000-5000 rpm for 10-15 minutes, taking supernate, filtering with a filtering membrane, concentrating and freeze-drying. According to the method, double-bacterium synchronous synergistic fermentation and a high-dose surfactant-assisted ultrasonic extraction technology are combined, so that a strong synergistic effect is formed, and the problems of low extraction rate, poor purity, high energy consumption, large solvent dosage and the like in a traditional loquat leaf ursolic acid extraction method are fundamentally solved.

The present application relates to ursolic acid with improved solubility and dispersibility, a method for producing same, and a method for improving the solubility of ursolic acid. In the present application, a complex of ursolic acid, an emulsifier, and a nonionic surfactant is formed and made into microparticles, thereby improving the solubility and dispersibility of poorly soluble ursolic acid and facilitating the utilization of ursolic acid as a nutritional material.

The invention discloses a leonurine hydrochloride and ursolic acid self-assembled nano preparation and application thereof in preparation of a medicine for treating acute lung injury. The invention provides a leonurine and ursolic acid self-assembled nano-preparation. The leonurine and ursolic acid self-assembled nano-preparation is obtained by self-assembling leonurine pharmaceutically acceptable salts and ursolic acid, wherein the amounts of the leonurine pharmaceutically acceptable salts and the ursolic acid are equal. The self-assembled nanoparticles have an obvious anti-lung inflammation effect, and the anti-inflammatory effect is obviously superior to that of a composition of the leonurine pharmaceutically acceptable salt and ursolic acid with the same dosage. Therefore, the leonurine and ursolic acid self-assembled nano preparation provided by the invention has the prospect of being developed into the medicine for treating the acute lung injury.

The present patent application describes a nutraceutical composition comprising ursolic acid, tomatine and polyphenols from grape pomace. Said composition resulted to be useful in counteracting muscle atrophy associated with neuropathic pain.

The invention belongs to the technical field of biology and new medicine, and discloses a preparation method of acoustic resonance driven glycyrrhizic acid nano-composite responsive gel, glycyrrhizic acid is used as a drug carrying system, berberine, ursolic acid, baicalein, luteolinin, curcumin, baicalin or ginsenoside Rh2 is used as a water-insoluble natural small molecule drug, and the acoustic resonance driven glycyrrhizic acid nano-composite responsive gel is prepared. Glycyrrhizic acid is an amphiphilic compound composed of one molecule of glycyrrhetinic acid and two molecules of glucuronic acid, a hydrophobic cavity can be formed in a water environment to wrap the water-insoluble natural small molecule medicine, a glycyrrhizic acid nano-composite solution is formed, and meanwhile the solubility of the water-insoluble natural small molecule medicine such as baicalein is improved.

The present application relates to the field of animal breeding technology, in particular to the application of ursolic acid in the frozen semen preservation of sheep. The present application applies ursolic acid in the frozen semen preservation of sheep. The addition of 0.5-2.0 micromole / L ursolic acid in the frozen diluent can improve the quality of frozen semen preservation of sheep and goats, and can improve the sperm motility and vitality after thawing, which helps the thawed sperm to pass through the vagina, uterus and enter the oviduct to complete fertilization, improves the insemination success rate of frozen sperm, and provides technical support for strengthening the protection of local sheep breed resources.

This invention provides a highly efficient extraction method for ursolic acid from loquat leaves and its application. The method includes the following steps: S1. Fresh loquat leaves are taken, washed clean, dried at 45℃ until the moisture content is <8%, pulverized, passed through a 60-mesh sieve, and microwave-sterilized to obtain sterilized loquat leaf powder; S2. The sterilized loquat leaf powder, yeast extract, MgSO4·7H2O, and water are mixed, and the pH is adjusted to 5.0 as a fermentation medium. Simultaneously, 5% *Saccharomyces cerevisiae* seed culture and 10% *Trametes versicolor* seed culture are inoculated and cultured at 30℃ and 150 rpm for 36-48 h with shaking; S3. A surfactant is added, stirred evenly, and ultrasonically extracted to obtain the extract; S4. The extract is centrifuged at 4000-5000 rpm for 10-15 min, the supernatant is filtered through a membrane, concentrated, and freeze-dried. This invention combines simultaneous synergistic fermentation by two bacteria with high-dose surfactant-assisted ultrasonic extraction technology, resulting in a powerful synergistic effect. This fundamentally solves the problems of low extraction rate, poor purity, high energy consumption, and large solvent consumption in traditional loquat leaf ursolic acid extraction methods.

The invention discloses an application of acetyl ursolic acid in promoting the growth of burkholderia. Eight Burkholderia strains are separated from soil, the separated eight Burkholderia strains are cultured in a nitrogen-free culture medium added with 50 mg / L of acetyl ursolic acid, and the result shows that the biomass of Burkholderia can be increased by 40%-95% compared with that of a control group without acetyl ursolic acid within 24 h through acetyl ursolic acid, the Burkholderia strain has a remarkable growth promoting effect on Burkholderia, and the Burkholderia strain has a good application prospect. The core functions such as nitrogen fixation are not influenced. The concentration gradient experiment further determines that the effective concentration range of the acetyl ursolic acid on the growth promotion of the burkholderia is 5-100 mg / L, and the optimal concentration of the acetyl ursolic acid is 25-75 mg / L. Acetyl ursolic acid is used as a specific growth promoting agent of burkholderia, not only are the problems of specificity, safety and stability in the prior art solved, but also the synergistic effect of promoting growth and improving crop quality is realized.

The invention discloses a pharmaceutical composition for adjuvant therapy of nodular diseases and a preparation method thereof, and relates to the technical field of medicines.The pharmaceutical composition is prepared from, by weight, 1-10 parts of cordycepin, 10-20 parts of spica prunellae extract, 10-30 parts of plant extract for softening hardness to dissipate stagnation and 15-30 parts of functional auxiliary materials; the cordyceps nucleoside is a nucleoside component of which the purity is greater than or equal to 98%; the mass ratio of oleanolic acid to ursolic acid in the prunella vulgaris extract is 3: 1, and the total content of oleanolic acid and ursolic acid is greater than or equal to 12%; the hard lump resolving plant extract is an extract of thunberg fritillary bulb, radix scrophulariae, oyster powder, seaweed, kelp, radix curcumae and radix bupleuri; the functional auxiliary material is formed by compounding at least three of a filling agent, a disintegrating agent, a suspending aid and an adhesive according to an equal mass ratio; the multiple raw materials are scientifically proportioned to play a synergistic role, the preparation process is standardized, active ingredients are reserved, indexes of finished products are stable, and large-scale production and adjuvant therapy of the nodular diseases can be achieved.

The present application provides a kind of epidermis repair gel dressings and its preparation method, it is related to dressing field.The epidermis repair gel dressings includes repair liquid, 100 parts of the repair liquid includes composite sodium hyaluronate 0.1-0.3 parts, carbomer 0.02-0.06 parts, glycerol 1-4 parts, pentanediol 1-3 parts, composite acid 0.05-0.1 parts, composite peptide 0.01-0.03 parts and the balance purified water by weight parts;The composite sodium hyaluronate is composed of sodium hyaluronate, hydrolyzed sodium hyaluronate and sodium hyaluronate crosslinked polymer;The composite acid is prepared from ursolic acid, hydroxyl asiatic acid, lipoic acid;The composite peptide is prepared from thirteen peptides and palmitoyl pentapeptide-3.The epidermis repair gel dressing of the present application has better repair effect on acne lesions, not only inhibits active propionibacterium acnes, but also can reduce the expression of inflammatory factors, can deeply repair damaged basal structure, maximumly reduce the degree of acne lesions, and restore to close normal skin.

The present application belongs to the technical field of quality control of traditional Chinese medicine, and particularly relates to a quality control method of Ledum palustre L. The quality control method provided by the present application comprises the following processes: S1, identification: thin layer chromatography identification is respectively performed by taking ursolic acid, hyperoside, rutin, aesculetin and aesculin as the control; S2, inspection: the moisture content, total ash content, acid insoluble ash content and alcohol-soluble extract content of the medicinal material are determined; S3, content determination: the total content of volatile oil, three five-ring triterpenes (betulinic acid, oleanolic acid and ursolic acid) and two flavonoid glycosides (hyperoside and preussin) in the medicinal material is determined by using high performance liquid chromatography. The present application combines multi-index component analysis and thin layer chromatography identification, has strong specificity and good reproducibility, can comprehensively and effectively control the quality of Ledum palustre L., and ensures the medicinal effectiveness and safety of Ledum palustre L.

The application relates to a process for extracting rosmarinic acid and application of the extracted rosmarinic acid in plant oil, S1, rosemary leaves are added into a mixed solution of ethanol and water with a first-stage concentration and are heated for extraction, after the extraction is completed, a first-stage extraction liquid is obtained; S2, the first-stage extraction liquid is concentrated, the concentrated liquid is subjected to plate-frame pressure filtration, the filter cake is washed with water, a filtrate and a filter residue are obtained; S3, the filtrate is subjected to extraction, the extraction liquid is concentrated, and after drying, a rosemary acid crude product is obtained; S4, the filter residue is subjected to reextraction with a mixed solution of ethanol and water with a second-stage concentration, after the reextraction is completed, filtration is carried out, a filtrate and a filter residue are obtained; the filter residue is a crude extract of ursolic acid and rosmarinic acid; S5, the filtrate is subjected to decolorization, and then is concentrated into a paste, after vacuum drying, a rosmarinic acid crude product is obtained. The method in the application can separate multiple active substances, obtain multiple crude products, improve the utilization rate of rosemary, and thus reduce the production cost.

The application provides application of ursolic acid lactone in prevention and treatment of Spodoptera exigua, and relates to the technical field of pest control. The ursolic acid lactone can produce significant antifeedant effect on Spodoptera exigua at a certain concentration, can inhibit the growth of Spodoptera exigua larvae to a certain extent, and can even cause the death of Spodoptera exigua larvae, and can be used as a new Spodoptera exigua control agent. The present application overcomes the shortcomings of the prior art, and the plant source extract component, i.e. ursolic acid lactone, can effectively control Spodoptera exigua, and the component is green and safe, reduces environmental pollution, and has a good prospect in the development of Spodoptera exigua control agents.

The present application relates to the technical field of fluorescent chemical sensor, in particular to a fluorescent compound for detecting ursolic acid and a preparation method and application thereof. The fluorescent compound for detecting ursolic acid has the structural formula shown in the following formula I: The fluorescent compound of the present application has the property of aggregation-induced emission, can realize rapid and sensitive 'lighting type' response to ursolic acid, and solves the problems of high cost, complex process and long time consumption in the prior art for detecting natural plant compounds.

Embodiments of the instant disclosure relate to novel methods and compositions for treating tumors resistant to platinum-based chemotherapy. In certain embodiments, methods of treating tumors herein can include administering an effective amount of at least one ursolic acid derivative. In certain embodiments, methods of treating tumors herein can include administering an effective amount of at least one ursolic acid derivative in combination with at least one platinum-based chemotherapeutic separately or in a combination therapy. In some embodiments, methods of treating tumors disclosed herein can include screening and / or selecting a subject suitable for treatment on the basis of SENP1 tumor expression. In other embodiments, methods of treating tumors can include administering a composition disclosed herein to a subject, the composition having a combination of at least one ursolic acid derivative and at least one platinum-based chemotherapeutic.

Compositions and methods are described for improving brain health and cognitive function and for preventing and treating Alzheimer's disease and other age-related dementia diseases and conditions for intake as a dietary or nutritional supplement, for example, as a powdered drink mix. Compositions for providing an energy source to the brain during exercise are also described. The composition includes a source of choline, a source of magnesium, at least one taurine-containing composition, a source of ashwagandha root, and phosphatidylserine. The source of choline can be alpha-glycerylphosphorylcholine or citicoline and the source of magnesium can be magnesium-L-threonate or magnesium glycinate. The composition can further include at least one of oleic acid, oleanolic acid, ursolic acid, curcumin, piperine, galantamine, huperzine-A, a source of vitamin B5, L-tyrosine, and beta-hydroxybutyrate.

The invention discloses application of ursolic acid or ursolic acid derivatives in preparation of chicken coccidiosis-resistant feed or chicken coccidiosis-resistant drugs, and relates to the technical field of biological medicines. The chicken coccidiosis protozoon is chicken eimeria tenella, the ursolic acid derivative is oleanolic acid, and the ursolic acid derivative and ursolic acid are isomers. The chicken coccidiosis-resistant feed or the chicken coccidiosis-resistant drug has at least one of the following functions: reducing the cecum lesion value and the number of oocysts, and inhibiting the depth increase of crypts induced by chicken coccidiosis; weight loss caused by chicken coccidiosis infection is relieved; the expression of inflammatory factors caused by chicken coccidiosis infection is reduced, and the inflammatory factors comprise IL-6, IL-8, TNF-alpha and IFN-gamma; the expression of tight junction protein after chicken coccidiosis infection is promoted, and the tight junction protein comprises ZO-1, Claudin-1 and Occludin. The traditional Chinese medicine composition has the advantages of low toxicity, low residue, good anti-chicken coccidiosis effect, simplicity in preparation, suitability for batch production and the like.

The invention relates to the field of functional foods, and discloses a preparation method of a composition for assisting in reducing blood sugar of diabetes based on shiny-leaved yellowhorn leaves. The composition comprises the following components in percentage by mass: 2.00% of rutin, 1.00% of ursolic acid, 3.00% of charantin, 0.05% of chromium picolinate, 2.00% of a mulberry leaf extract, 2.00% of puerarin, 8.00% of an astragalus membranaceus extract, 10.00% of Chinese yam powder, 2.00% of a ginseng extract, 20.00% of oligosaccharide, 48.45% of soluble dietary fibers, 1.00% of erythritol and 0.50% of microcrystalline cellulose. The Xanthoceras sorbifolia leaf double-component extraction is adopted, multiple natural functional factors and a low-GI matrix are matched, and the effect of assisting in reducing blood sugar is achieved through multi-channel synergy of regulating intestinal flora, delaying sugar absorption, enhancing insulin sensitivity and the like. The composition has the advantages of high safety and natural components, can be industrially granulated and produced, and is suitable for auxiliary conditioning of prediabetic people and patients with type 2 diabetes mellitus. The invention also discloses a preparation method and action verification data of the composition.

The application of ursolic acid in the preparation of drugs for treating liver fibrosis can effectively solve the problem of medication for treating liver fibrosis. By regulating the differentiation of immune cells (Th17 cells) and reducing inflammatory damage, ursolic acid intervenes in the liver's immune microenvironment, effectively reversing liver fibrosis, and thus enabling its application in the preparation of drugs for treating liver fibrosis. This invention develops a new drug for treating liver fibrosis, opening up a new avenue for medication in the treatment of liver fibrosis. It represents a major innovation in the treatment of liver fibrosis, demonstrating good efficacy, safety, and practical application value.

The application discloses a use of ursolic acid as a SARS-CoV-2 subunit vaccine adjuvant, aims to solve the technical problems of relatively weak immunogenicity of existing subunit vaccines, possible local or systemic adverse reactions of some adjuvants and unclear action mechanism, and provides a new medical use of ursolic acid. The ursolic acid can effectively improve the antigen-specific antibody level of the subunit vaccine immunized mice, promote the formation of germinal centers and enhance follicular helper T cell response. The action mechanism is that after the ursolic acid is combined with PTPRF, the stability of the PTPRF is affected and the degradation of the PTPRF is promoted, thereby enhancing the downstream STAT3 phosphorylation, promoting the expression of antigen presentation related genes and improving the antigen presentation ability of the dendritic cells, and finally enhancing the antibody response of the body. The application provides a new subunit vaccine adjuvant candidate molecule for preventing severe SARS-CoV-2 related infections in clinic, and provides a new molecular target and mechanism basis for the development of subunit vaccine adjuvants.

The application belongs to the field of biological medicine, and particularly relates to a Bama pig model for preclinical research of a weight loss drug COST and application. Healthy Bama pigs are first adaptively fed with ordinary feed, and then fed with high-fat feed to model, and the Bama pigs are detected for obesity-related indexes at a fixed time period every week; the high-fat feed comprises ordinary feed, beef tallow, artificial butter, sucrose, casein, experimental animal premix, microcrystalline cellulose, calcium bicarbonate, gypenoside, peppermint leaf, maslinic acid, ursolic acid, atractylodes, nutmeg. The application can quickly construct a Bama pig model with stable phenotype, and provides a reliable model for preclinical research of a new drug for treating obesity, and provides guarantee for research on the mechanism of obesity, screening, development and mechanism research of a new drug for treating obesity and the like.

The invention belongs to the technical field of biological medicines, and particularly relates to application of aescin in preparation of a product for inducing autophagy of skin cells. According to the application disclosed by the invention, research finds that aescin can induce autophagy of skin keratinocytes for the first time, and the expression level of autophagy-related proteins LC3-I and LC3-II in the skin keratinocytes can be remarkably improved; in addition, aescin and ursolic acid have a synergistic effect in the aspect of inducing skin keratinocyte autophagy, and are safe and non-irritant to skin. Therefore, the aescin and the composition of the aescin and the ursolic acid can be used for preparing products for inducing autophagy of skin cells so as to resist aging, remove freckles, whiten skin, control oil and strengthen a skin cuticle barrier, and products for slowing down a scleroderma process / relieving a scleroderma symptom, slowing down an atopic dermatitis process / relieving an atopic dermatitis symptom and relieving acne. The traditional Chinese medicine composition has the advantages of remarkable curative effect and high safety.

The invention provides arbutin-28beta-pyroglutamyl ester. The arbutin-28beta-pyroglutamyl ester is prepared by adopting a two-stage liquid fermentation process to culture a Circinella muscae CGMCC (China General Microbiological Culture Collection Center) 3.2695 strain. The arbutin-28beta-pyroglutamyl ester is a natural triterpenoid metabolite synthesized through microbial conversion for the first time, has good activity of promoting the growth of motor neurons, and can be used for preparing drugs for treating nervous system diseases.

The application discloses a detection method and application of ursolic acid and total triterpenes in prunella vulgaris seed and prunella vulgaris seed oil. The detection method is characterized in that ursolic acid in prunella vulgaris seed and prunella vulgaris seed oil is qualitatively identified by using a thin layer chromatography method; oil and fat components interfering with determination of total triterpenes are separated and removed by column separation, and then the content of total triterpenes in prunella vulgaris seed and prunella vulgaris seed oil is determined by using spectrophotometry with ursolic acid or oleanolic acid as a control, so that the problems of interference and false high content in determination of the content of total triterpenes in oil components in the prior art are overcome. The method has the advantages of high accuracy and precision, good stability and repeatability, and provides a method for quality detection of triterpenes in development and utilization of prunella vulgaris seed and prunella vulgaris seed oil, and has strong specificity and good reproducibility.