1429 results about "Skin barrier" patented technology

The invention belongs to the technical field of protein engineering and genetic engineering, and particularly relates to recombinant IV-type humanized collagen as well as a preparation method and application thereof. A section of brand new recombinant collagen is designed and obtained through methods such as screening, replacement and splicing, the nucleotide sequence of the recombinant collagen is optimized through pichia pastoris codon preference, then a corresponding genetic engineering yeast strain is constructed and obtained, and the recombinant collagen is successfully prepared through fermentation. Experiments prove that the recombinant IV-type humanized collagen prepared by the invention is large in molecular weight and high in yield, and compared with commercially available recombinant collagen, the recombinant IV-type humanized collagen has better free radical scavenging performance and cell proliferation and migration promoting performance, and has good antioxidant and skin barrier repair functions, so that the recombinant IV-type humanized collagen has good practical application value.

The invention provides an anti-dandruff composition based on double barrier repair as well as a preparation method and application thereof, and relates to the technical field of shampoo supplies, the anti-dandruff composition comprises the following effective components: at least two of isodon amethystoides extract, polygonum capitatum extract and myrobalan extract. The compound has a synergistic antibacterial effect, still has a synergistic antibacterial effect after being compounded with OCT, and can be used for replacing or cooperating with OCT, so that the dosage of OCT is reduced, and the irritation is reduced; the anti-dandruff composition has the advantages that the anti-dandruff effect is good, the DPPH free radical scavenging capacity of the anti-dandruff composition is better than that of OCT, the isodon amethystoides extract, the polygonum capitatum extract and the fructus chebulae extract are plant extracts and have no irritation to scalp, the whole anti-dandruff composition has no irritation to the scalp, and the technical problems that in the prior art, anti-dandruff products have certain influences on skin barriers, and the long-acting anti-dandruff effect cannot be achieved are solved.

The present invention provides a Lactiplantibacillus plantarum ( Lactiplantibacillus plantarum ), strain 24, which is deposited in the China General Microbiological Culture Collection Center, deposit number: CGMCC No. 31278. The Lactiplantibacillus plantarum 24 of the present invention can secrete sphingomyelinase to promote the production of ceramide in HaCaT cells; Lactiplantibacillus plantarum 24 or its metabolite or its lysate can up-regulate the expression of key enzymes in the sphingomyelinase and / or ceramide synthesis pathway of skin cells, repair the skin barrier; down-regulate the expression of pro-inflammatory cytokines, inhibit autophagy, and has a good anti-inflammatory effect; at the same time, it also has good antioxidant and whitening effects. The Lactiplantibacillus plantarum 24 or its metabolite or its cell lysate of the present invention can be used in the preparation of products having the above-mentioned effects or functions.

The invention provides a composition with oil-controlling, acne-removing, barrier-repairing effects and application thereof, and belongs to the field of cosmetics. The composition is prepared from a baropetalum beetle leaf extract, a roselle flower extract, totarol and a cryptomeria fortunei bud extract. The baropetalum chinense leaf extract has the effects of balancing grease secretion and shrinking pores; the roselle flower extract is rich in organic acid and anthocyanin, and has the effects of dissolving pore keratosuppository, controlling oil, resisting inflammation, smoothing acne skin, preserving moisture and removing redness; as a bacteriostatic agent, totarol has a natural preservative effect on gram-positive bacteria and partial negative bacteria, and has the effects of removing acnes, sterilizing, diminishing inflammation, whitening and the like; the cryptomeria fortunei bud extract can promote skin tissue structure regeneration and strengthen skin barriers. The composition effectively regulates and controls excessive secretion of sebaceous gland grease and pox skin specific protein through the synergistic effect of various components, promotes the health of pox skin, repairs the skin barrier and enhances the self-protection ability of the skin at the same time.

The invention discloses pediococcus pentosaceus CCFM1402 capable of effectively repairing a skin barrier and a self-fermentation metabolite of the pediococcus pentosaceus CCFM1402, and belongs to the technical field of microorganisms and medicines. The pediococcus pentosaceus CCFM1402 provided by the invention has the effect of improving the skin barrier function in both external use and oral administration; the cell activity of damaged HaCaT cells and the expression of the gene level of skin barrier function protein and tight junction protein can be improved by virtue of a thallus lysate and a fermented supernatant (metagen) of the external pediococcus pentosaceus CCFM1402; and the DNFB-induced mouse skin barrier injury can be relieved in the aspects of appearance, skin barrier protein expression and the like by orally taking viable bacteria and postbiotics of the pediococcus pentosaceus CCFM1402. Therefore, the pediococcus pentosaceus CCFM1402 has a huge application prospect in preparation of external or oral medicines for repairing the skin barrier and preparation of food, health care products or cosmetics.

The invention provides an acne-removing and relapse-preventing composition suitable for acne sensitive skin and application of the acne-removing and relapse-preventing composition, and belongs to the technical field of cosmetics. The epigallocatechin gallic acid glucoside, the brazilin, the roselle flower extract, the jujube bark extract, the linseed oil unsaponifiable matter and the guaiacol extract are selected as active ingredients of the acne-removing and relapse-preventing composition and interact with one another, growth of acne-related pathogenic bacteria can be rapidly inhibited, and the skin micro-ecology can be adjusted; skin inflammation in the whole process of acne is effectively inhibited, the effects of preventing and inhibiting inflammation are achieved, the skin barrier is effectively repaired, redness is reduced, meanwhile, the effect of adjusting skin grease balance is achieved, and acne recurrence is prevented; in addition, the acne-removing and relapse-preventing composition also has the effects of removing yellow gas, uniformizing skin color and improving skin roughness, is mild and non-irritating, can be used for sensitive skin, and expands the application range of the acne-removing and relapse-preventing composition.

The invention relates to a novel application of animal bifidobacterium subsp. Lactis BGI-N3, in particular to an application of the animal bifidobacterium subsp. Lactis BGI-N3 in preparation of a preparation for relieving skin allergy. The invention finds that the bifidobacterium animalis subsp. Lactis BGI-N3 has a very remarkable effect in the aspect of relieving skin allergy, and the effect is specifically shown as follows: in an atopic dermatitis animal model, the dermatitis score, scratching times and percutaneous water loss are remarkably reduced, and the atopic dermatitis skin tissue damage condition is relieved; the immune organ edema condition is improved, the levels of Th2 type cell factors and inflammatory factors IL-6, TNF-alpha and IFN-gamma related to dermatitis are reduced, and the immune balance of the body is recovered; expression of fibroin and endothelial protein in skin tissue is improved, and the skin barrier is repaired; the level of antibacterial peptide in skin tissues is recovered, and the antibacterial activity of the skin is enhanced. Meanwhile, compared with a traditional chemical medicine for treating atopic dermatitis, the animal bifidobacterium subsp. Lactis BGI-N3 has no side effects such as weight loss, immune organ atrophy, immune imbalance and the like.

The invention provides a lactobacillus plantarum 24 which is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms, and the preservation number of the lactobacillus plantarum 24 is CGMCC No.31278. The lactobacillus plantarum 24 has the advantages that the lactobacillus plantarum 24 is prepared from lactobacillus plantarum, lactobacillus plantarum, lactobacillus plantarum, lactobacillus plantarum, lactobacillus plantarum, lactobacillus plantarum, lactobacillus plantarum and lactobacillus plantarum; the plant lactobacillus 24 disclosed by the invention can secrete sphingomyelinase and promote HaCaT cells to generate ceramide; the plant lactobacillus 24 or the metabolite or lysate thereof can up-regulate expression of key enzymes in a synthetic route of skin cell sphingomyelinase and / or ceramide, and repair a skin barrier; expression of proinflammatory cytokines is lowered, autophagy is inhibited, and a good anti-inflammatory effect is achieved; meanwhile, the anti-oxidation and whitening effects are relatively good. The plant lactobacillus 24 or the metabolite or the cell lysate of the plant lactobacillus 24 can be applied to preparation of products with the effects or the effects of the plant lactobacillus 24 and the metabolite or the cell lysate of the plant lactobacillus 24.

The invention relates to the field of cosmetics, and discloses an anti-wrinkle composition containing recombinant collagen as well as a preparation method and application of the anti-wrinkle composition. The anti-wrinkle composition is prepared by compounding I-type collagen peptide nano-micelles, recombinant humanized III-type collagen, recombinant humanized XVII-type collagen, glycosylglycerol and ceramide. The skin care composition has the advantages that multiple synergistic effects are achieved, collagen generation is promoted, the skin moisturizing capacity is enhanced, and the skin barrier is repaired, so that the elasticity, compactness and smoothness of the skin are remarkably improved. The product disclosed by the invention has the effects of skin barrier repair, anti-aging care, collagen protection, collagen generation promotion, water locking and moisturizing and the like, and is suitable for research, development and application of skin care products for resisting wrinkles, tightening and delaying senescence.

The invention relates to taurine cross-linked salicylic acid nano-composite gel for efficient transdermal delivery and mild acne removal as well as a preparation method and application of the taurine cross-linked salicylic acid nano-composite gel. The nano-gel comprises salicylic acid, quaternary ammonium salt-73, taurine, polyol, lipid, an emulsifier, a co-emulsifier, an antioxidant and water. Salicylic acid, quaternary ammonium salt-73 and taurine are used as functional components for removing acnes. Salicylic acid can decompose grease on the surface of the skin, so that the oil control effect is achieved, meanwhile, skin metabolism is promoted, angular suppository is loosened, pores are dredged, and acne is treated; quaternary ammonium salt-73 has extremely high antibacterial activity, especially has a remarkable antibacterial effect on propionibacterium acnes causing acnes, and can quickly and thoroughly remove the acnes by being matched with salicylic acid; taurine provides energy required by normal physiological activities of human cells, improves skin immunity, regulates skin water-oil balance and has the effects of improving skin textures, dryness, wrinkles, elasticity, sensitivity and other skin problems, meanwhile, in the using process, taurine is preferentially absorbed by the skin, so that an inclusion shell is broken, and the skin is protected. Rapid release and transdermal absorption of salicylic acid and quaternary ammonium salt-73 are promoted, irritation is reduced, and the three components act synergistically to achieve the functions of mildly, efficiently and rapidly treating acne and reconstructing skin barrier.

The invention relates to a composition containing ceramide and collagen tripeptide and a preparation method thereof, and belongs to the technical field of health care products. The invention discloses a composition containing ceramide and collagen tripeptide. The composition is prepared from the following raw materials: collagen tripeptide, rice bran oil powder, a herba houttuyniae extract, pineapple powder, konjaku flour, a grape seed extract, olive fruit powder, carnosine, pomegranate concentrated powder and a whey fermentation product. The grape seed extract, the olive fruit powder, the carnosine and the pomegranate concentrated powder inhibit the Maillard reaction of the collagen peptide and improve the utilization rate of collagen tripeptide; ceramide rich in the rice bran oil powder is a natural skin moisturizing factor, and is matched with the pineapple powder and the konjaku flour which have a keratin metabolism effect to maintain the health of cuticles; the herba houttuyniae extract and the whey fermentation product are matched to have an excellent anti-inflammatory effect, so that the components enhance the skin barrier in multiple dimensions through collagen synthesis, cuticle maintenance, anti-inflammatory and anti-oxidation.

The invention discloses a composition with tightening, wrinkle fading, soothing and repairing effects and application of the composition. The composition with the effects of tightening and fading wrinkles and relieving and repairing is prepared from a lactobacillus fermentation product, hexanediol, fully human collagen, glycosylglycerol, a citrus peel extract, a palmitoyl tripeptide-5 solution, a chrysanthemum extract and SPG beta-glucan. According to the invention, seven active material raw materials including the lactobacillus fermentation product, the fully human collagen, the glycosylglycerol, the citrus peel extract, the palmitoyl tripeptide-5 solution, the chrysanthemum extract and the SPG beta-glucan are creatively combined for use, and the seven raw materials supplement each other and have a synergistic effect; the effects of lightening skin wrinkles, improving skin barriers and relieving skin sensitivity are achieved from multiple dimensions, and cosmetics prepared from the composition have the effects of repairing, relieving, tightening and resisting wrinkles.

This invention provides a transdermal nanoparticle delivery system for skin anti-inflammation, comprising a protein-blue calyx methyl ether-polymer shell capsule composite structure. The protein has an affinity for the skin, blue calyx methyl ether is loaded within the protein, and the polymer layer coats the surface of the protein. The blue calyx methyl ether nanoparticle transdermal delivery system has a size of 30-80 nm, and the polymer shell thickness is 10-35 nm. The surface properties of the polymer shell can be controlled according to the required transdermal delivery depth, enabling the protein to penetrate the skin barrier and be efficiently delivered deep into the skin, where the blue calyx methyl ether is then slowly released, targeting and alleviating skin inflammation. This addresses the drawback of low therapeutic efficacy of blue calyx methyl ether in treating skin inflammation due to unsatisfactory transdermal effects.

The invention relates to an aloe extract nano-vesicle as well as a preparation method and application thereof, and belongs to the technical field of cosmetics. The aloe extract nano-vesicle provided by the invention is a nano-micelle formed by an aloe extract and tocopheryl acetate, the particle size of the aloe extract nano-vesicle is 280 to 500 nm, the Zeta potential of the aloe extract nano-vesicle is-10.12 to-11.34 mV, and the polydispersity coefficient of the aloe extract nano-vesicle is 0.239 to 0.434. The aloe extract nano-vesicle disclosed by the invention has a nano-scale size, and can easily penetrate through a skin barrier and enter skin cells, so that the moisturizing effect of the aloe extract nano-vesicle is achieved. The permeability of the moisturizing composition is far better than that of the traditional moisturizing composition, so that the required moisture can be more effectively provided for the skin. The aloe extract nano-vesicle can carry other active ingredients such as moisturizing factors to deeply permeate into skin, comprehensive care is provided for the skin, the texture and appearance of the skin are improved, the preparation process is simple, and the aloe extract nano-vesicle is suitable for technological production.

The invention relates to bionic skin barrier cream with a three-layer phospholipid structure and a preparation method of the bionic skin barrier cream, and belongs to the technical field of cosmetics, the bionic skin barrier cream comprises the following raw materials: lecithin, wax ester, triglyceride, composite ceramide, phytosterol oleate, squalane, an antioxidant, polyol, composite amino acid, pyrrolidone carboxylic acid, urea and arginine. A stabilizing agent, a treating agent and the balance of deionized water. The bionic skin barrier cream prepared by the invention has a three-layer phospholipid structure, and compared with a double-layer phospholipid structure in the market, the firmness and hydrophobicity of the double-layer structure are further improved by the added third-layer structure, so that the prepared bionic skin barrier cream has outstanding waterproof, sweat-resistant and light pollution-resistant effects; in addition, the wax ester, the triglyceride, the phytosterol oleate, the pyrrolidone carboxylic acid and the urea selected in the invention have a synergistic effect, and can generate excellent moisturizing and repairing effects and certain protection effects.

The invention discloses a composition with high ultraviolet damage resistance as well as a preparation method and application thereof, and particularly belongs to the technical field of skin care product raw materials. The composition with high ultraviolet damage resistance is prepared by performing solid-state fermentation on radix scutellariae roots, radix ligustici sinensis and radix puerariae through compound bacteria consisting of bacillus subtilis, lactobacillus bulgaricus and bacillus coagulans and then performing solvent extraction. The composition with high ultraviolet damage resistance has excellent effects of resisting oxidative damage and inhibiting inflammatory factors, and can achieve the effects of repairing skin barriers, resisting inflammation, relieving, efficiently promoting repair of after-sun skin and the like.

The present invention belongs to the technical field of protein engineering. Provided are a recombinant type XVII collagen having a triple helix structure and the use thereof. The amino acid sequence of the recombinant type XVII collagen comprises n core units, wherein n is the integer 1 or an integer greater than 1, and the amino acid sequence of the core units is as shown in SEQ ID NO: 1. Further provided are a method for constructing a plurality of expression systems and a method for preparing the recombinant type XVII collagen. Further provided is the actual industrial application on the basis of the characteristics of the protein. The provided recombinant type XVII collagen exhibits high purity, good thermal stability and high biological activity, and has a molecular weight much smaller than that of natural collagen, thereby reducing the difficulty for the collagen to cross the skin barrier and exert biological functions. Experiments show that the recombinant type XVII collagen has a variety of biological activities such as promoting cell migration.

The invention relates to the technical field of cosmetics, in particular to application and a synthesis method of cyclic peptide. The inhibition objects of the cyclic peptide comprise IL-1beta, IL-6 and TNF-alpha (Tumor Necrosis Factor-alpha); when the concentration is less than or equal to 10ppm, the inhibition rate on IL-1beta is greater than or equal to 40%, the inhibition rate on IL-6 is greater than or equal to 50%, and the inhibition rate on TNF-alpha is greater than or equal to 40%. The cyclopeptide has a barrier repairing effect and can improve FLG expression; meanwhile, the moisturizing effect is achieved, and percutaneous moisture loss of the skin can be reduced. The invention provides a group of cyclic peptides, which has excellent anti-inflammatory, moisturizing, barrier repairing and soothing effects, and compared with the existing straight-chain peptides in the market, the group of cyclic peptides has more obvious anti-inflammatory and soothing effects, and has a certain skin barrier repairing effect. The group of cyclic peptides can be applied to the fields of cosmetics and skin care products, and the product efficacy is improved; and the synthetic method of the cyclic peptide is easy to implement and wide in application prospect.

The invention provides a preparation method of a recombinant collagen dressing, and belongs to the technical field of medical dressings. According to the technical scheme, stacked short sequences and continuous human collagen amino acid short sequences are repeatedly combined for multiple times to form recombinant collagen with specificity, and high similarity of the recombinant collagen with human collagen is ensured. On the basis, 4-FPBA is used as a cross-linking agent to respectively form a Schiff base bond responsive to pH and a boric acid ester bond responsive to glucose with CMCS and two kinds of HA with different molecular weights, and then the double-dynamic covalent cross-linked network adhesive hydrogel is synthesized. The triple-helix structure of the recombinant collagen is combined with a specific receptor on a cell membrane, and cell behaviors are regulated and controlled by inducing signal transduction through a cytoskeleton, so that skin microcirculation can be effectively improved, and a skin barrier can be repaired; the hydrogel dressing matrix has the effects of resisting inflammation, resisting oxidation, resisting bacteria, promoting healing and the like, the biological performance of the dressing is remarkably improved, and a better curative effect is achieved.

The invention relates to a composition with anti-allergy, repairing and moisturizing effects. The composition is prepared from fibronectin, kava pepper leaf / root / stem extract, ectoine, hydroxyphenyl propionamide benzoic acid, wheat fine powder lipid and bio-saccharide gum-1. The specific components are matched with one another, inflammatory response can be inhibited from multiple aspects, the skin can be sufficiently relieved, the redness phenomenon is reduced, meanwhile, the skin barrier is enhanced and repaired, long-term moisturizing is achieved, and the excellent anti-allergy, repairing and moisturizing effects are achieved.

The invention provides recombinant human III-type collagen as well as a preparation method and application thereof, and belongs to the technical field of protein engineering, the amino acid sequence of the recombinant human III-type collagen is shown as SEQ ID No.1, and the nucleotide sequence of a gene for coding the recombinant human III-type collagen is shown as SEQ ID No.2; the invention also provides a method for expressing the recombinant human III type collagen in mammalian cells. The recombinant human III-type collagen provided by the invention is high in purity, has triple helix characteristic circular dichroism, has the molecular weight of 21.8 kDa, is far smaller than that of natural full-length collagen, has the effects of promoting cell proliferation and cell migration, and reduces obstacles for the collagen to penetrate through a skin barrier to exert biological functions.

Provided are methods and composition for treating skin barrier functions and associated disorders. Formulated compositions may be applied on human skin to maintain a desired skin surface pH over an extended period of time without irritating the skin. Maintaining the desired surface pH aids in treatment of eczema, in addition to other skin conditions.

The invention provides essential oil containing ceramide and a preparation method of the essential oil. The essential oil containing ceramide is prepared from ceramide, mannosyl erythritol lipid, synthetic grease and natural vegetable grease. According to the ceramide-containing essential oil disclosed by the invention, complex components such as a surfactant and a stabilizer do not need to be added, and the added mannosyl erythritol lipid is natural sugar ester and has good biocompatibility and safety, so that the ceramide-containing essential oil disclosed by the invention is mild and non-irritant to skin and has excellent product safety; besides, according to the pure oil formulation formula, the purpose of improving the solubility and stability of ceramide can be achieved only by adding mannosyl erythritol lipid, the skin barrier maintaining effect of ceramide can be better played, the anti-inflammatory and skin repairing effects of ceramide are improved, and the skin health level is improved; the application range of ceramide in cosmetics is expanded.

The invention relates to application of cembratrienol in preparation of a medicine for preventing and / or treating psoriasis, and belongs to the technical field of biological medicine. The invention provides an application of cembratrienol in preparation of a medicine for preventing / or treating psoriasis. Researches find that cembratrienol can significantly inhibit secretion of inflammatory factors in a psoriasis-like cell model induced by M5; moreover, research finds that cembratrienol can significantly improve the skin lesion condition of an imiquimod-induced psoriasis mouse model, improve the weight loss degree of the psoriasis mouse model, relieve the pruritus condition of the psoriasis mouse model, improve the skin inflammation of the psoriasis mouse model, repair the skin barrier of the psoriasis mouse model, and improve the curative effect of the psoriasis mouse model. The body immune response of the psoriasis mouse model is reduced. Therefore, cembratrienol has a great application prospect in preparation of the medicine for preventing / treating psoriasis.

The invention discloses a barrier repair composition applicable to dry sensitive skin and application thereof. The composition comprises a golden camellia extract, a loquat leaf extract, an alfalfa extract and hydrolyzed royal jelly protein. According to the barrier repairing composition applicable to the dry sensitive skin, disclosed by the invention, through reasonable matching and synergistic interaction of all the components, a better skin barrier repairing effect can be achieved, so that the problems of dry and tight skin, desquamation, erythema, itching, dull luster, redness, burning heat sensation, pricking pain and the like of the dry sensitive skin are fundamentally solved.

The invention relates to a silk fibroin gel dressing and a preparation method and application thereof, the silk fibroin gel dressing comprises a silk fibroin gel dressing main body formula, and the silk fibroin gel dressing main body formula comprises water, silk fibroin, an inducer, a protective agent and a thickening agent; wherein the mass fraction of the silk fibroin is 0.1%-5%, the mass fraction of the inducer is 1%-40%, the mass fraction of the protective agent is 1%-10%, the mass fraction of the thickening agent is 0.1%-2%, and the balance is water. The prepared silk fibroin gel dressing is easy to smear and absorb and does not need to be cleaned; compared with traditional dressings, safer components are adopted, and the dressing is mild, painless and non-irritant; through the synergistic effect of the various components, multi-dimensional protection can be formed on the skin surface, the skin barrier injury can be repaired, the effects of moisturizing, relieving, red fading and stability maintaining are achieved, postoperative skin discomfort can be relieved, and wound healing can be promoted.

The invention provides an in-vitro skin blood vessel immune model as well as a preparation method and application thereof. THP-1 human monocyte leukemia cells are induced to be differentiated into M0 type macrophages, and then the M0 type macrophages are respectively induced into M1 type macrophages and / or M2 type macrophages; then co-culturing the M1 type and / or M2 type macrophages and vascular endothelial cells to form a vascular immune model; finally, the 3D skin model is placed on the blood vessel immune model, external stimulation is conducted, and the in-vitro skin blood vessel immune model is constructed. The in-vitro skin blood vessel immune model can be used for repairing skin barriers, inflammation pathways, blood vessel metabolism, the expression level of genes or proteins related to extracellular matrixes, vascular endothelial cells, the proliferation and migration ability of activated macrophages and the like. The seven feature dimensions of the physiological structure feature of the skin model are used for screening the to-be-tested sample and exploring the action mechanism, and the method has the characteristics of rapidness, multiple screening dimensions, high accuracy, low construction difficulty, low cost and high universality.

The invention relates to an aromatic soluble polymer microneedle as well as a preparation method and application thereof. The preparation method comprises the following steps: adding a zinc acetate hexahydrate aqueous solution and a Tris buffer solution into a eugenol-curcumin mixed ethanol solution to obtain self-assembled carrier-free nanoparticles containing eugenol and curcumin; and dissolving the nano-particles in a soluble hyaluronic acid solution, drying in a microneedle mold, and demolding to obtain the aromatic soluble polymer microneedle. The structure of the aromatic soluble polymer microneedle is that self-assembled carrier-free nanoparticles are wrapped in a soluble hyaluronic acid microneedle. Compared with the prior art, the aromatic soluble polymer microneedle prepared by the invention can overcome skin barrier and quickly absorb moisture and dissolve so as to promote the transdermal absorption effect of active substances; a perfume delivery system is constructed by using the soluble polymer microneedle, so that the biocompatibility is improved, the slow release control capability of the perfume can be effectively enhanced, and a new path is opened up for clinical application of inflammatory diseases.

A biosynthesis method for preparing royal jelly acid and the use thereof in skin care, which belong to the technical field of genetic engineering and biosynthesis. The royal jelly acid is produced by means of fermentation on the basis of yeast engineering bacteria using a culture medium containing trans-2-decenoic acid. An assessment at both skin cell level and skin model level shows that royal jelly acid can promote the synthesis of stratum corneum lipids and ceramide on aging skin and repair the skin barrier; and also can promote the synthesis of mitochondria ATP and NADPH in skin fibroblasts, reduce the active oxygen content of mitochondria, improve mitochondrial dysfunctions, and inhibit apoptosis. Further experiments show that royal jelly acid can repair DNA damage of fibroblasts caused by ultraviolet irradiation, reduce telomere shortening caused by cell division and replication, and strengthen the protection of chromosomal terminal regions. The prepared royal jelly acid has wide application prospects in the development of functional skin care products for skin repairing, aging resisting, etc.

The invention relates to a long-acting whitening and blackening-free composition containing glabridin and pterostilbene and application of the long-acting whitening and blackening-free composition, and belongs to the technical field of whitening cosmetics. The composition provided by the invention is prepared from the following components in parts by weight: 0.005 to 0.1 part of glabridin, 0.03 to 0.4 part of pterostilbene, 0.02 to 0.2 part of herba centellae extract, 0.0005 to 0.005 part of palmitoyl tripeptide-8 and 0.01 to 0.1 part of sodium hyaluronate. The application of the composition provided by the invention comprises the following steps: (1) preparing a phase A containing an inclusion empty shell; (2) dissolving glabridin, pterostilbene and palmitoyl tripeptide-8 to obtain a phase B; dissolving the centella asiatica extract and sodium hyaluronate to obtain a phase C; (3) adding the phase A and the phase C into the phase B, and uniformly mixing to obtain an inclusion phase; and (4) compounding the inclusion phase with a cosmetic base material. The composition provided by the invention integrates melanin regulation and control and skin barrier repair, enhances the whitening effect through targeted transmission and inclusion slow release, prolongs the blackening time, and has a very good application prospect in the whitening cosmetic industry.