The invention discloses a
cryptomeria fortunei clone in-vitro rooting culture method. The method comprises steps as follows: 1), a
tissue culture material is disinfected and cultured in a selected flask; 2), the
tissue culture material is inoculated into a subculture multiplication medium for subculture multiplication, adventitious buds are induced and differentiated and then transferred into an MS (Murashige and Skoog) medium for elongation culture, the formula of the subculture multiplication medium adopts DCR, 1.0mg / L 6-BA, 0.05 mg / L of NAA and 3% of
sugar; 3) when the adventitious buds elongate to 2-3 cm, the adventitious buds are transferred to a root induction medium for culture, a rooting test-tube
plantlet is obtained, and the root induction medium adopts 1 / 2 DCR, 0.05-0.2mg / L of IBA, 0.05-0.2 mg / L of NAA and 2% of
sugar; and 4), the rooting test-tube
plantlet is transplanted. According to the method, coppice
shoot is taken as a
tissue culture material, and adventitious
bud induction is performed through subculture multiplication; and when the adventitious buds elongate to 2-3 cm, 1 / 2 DCR is taken as a basic medium, a
plant growth regulator is added for root induction, the average rooting percentage is 84% and can be up to 93.3% to the maximum, the average rooting number of each
plantlet is 5.7, and the average
survival rate of
transplanting is larger than 86%.