64 results about "Cryptomeria" patented technology

The invention relates to the technical field of skin-care products, in particular to a composition containing a cryptomeria japonica bud extract for skin regeneration of a human face with steroid acnes and a preparation method of the composition. The composition comprises the following raw materials in parts by weight: 10-20 parts of the cryptomeria japonica bud extract, 20-40 parts of plant anti-sensitivity essence, 4-8 parts of glycosphingolipids, 20-30 parts of flowing spring factors, 5-15 parts of desert plant moisturizing factors, 15-25 parts of sodium hyaluronate and 4-8 parts of oat beta-glucan. The composition is good in antioxidant effect, awakens dormant cells, enables skin cells to slowly 'wake up', and has a rejuvenating effect; and furthermore, the skin can be safe against damage of an external environment, injury to sensitive skin by severe environment conditions can also be avoided, meanwhile, effects of astringing and controlling oil, and calming skin are achieved, andthus, the skin can be smooth and bright.

The invention discloses a cryptomeria fortunei clone in-vitro rooting culture method. The method comprises steps as follows: 1), a tissue culture material is disinfected and cultured in a selected flask; 2), the tissue culture material is inoculated into a subculture multiplication medium for subculture multiplication, adventitious buds are induced and differentiated and then transferred into an MS (Murashige and Skoog) medium for elongation culture, the formula of the subculture multiplication medium adopts DCR, 1.0mg/L 6-BA, 0.05 mg/L of NAA and 3% of sugar; 3) when the adventitious buds elongate to 2-3 cm, the adventitious buds are transferred to a root induction medium for culture, a rooting test-tube plantlet is obtained, and the root induction medium adopts 1/2 DCR, 0.05-0.2mg/L of IBA, 0.05-0.2 mg/L of NAA and 2% of sugar; and 4), the rooting test-tube plantlet is transplanted. According to the method, coppice shoot is taken as a tissue culture material, and adventitious bud induction is performed through subculture multiplication; and when the adventitious buds elongate to 2-3 cm, 1/2 DCR is taken as a basic medium, a plant growth regulator is added for root induction, the average rooting percentage is 84% and can be up to 93.3% to the maximum, the average rooting number of each plantlet is 5.7, and the average survival rate of transplanting is larger than 86%.

The invention discloses insect-prevention crispy snakegourd seeds. The insect-prevention crispy snakegourd seeds are prepared from the following substances: snakegourd seeds, tea leaves, fineleaf schizonepeta herb, anises, radix stemonae, polygala tenuifolia, liquorice roots, Chinese angelica, poria cocos, cortex dictamni, melia azedarach leaves, streptocaulon griffithii hook, auricled hedyotis herb, chrysopogon zizanioides, pelargonium graveolens, cryptomeria japonica and an additive; and the additive is prepared from the following substances: table salt, cyclamate, citric acid, lycium barbarum polysaccharides, polyporus polysaccharides and multiplex vitamin of electrolyte. The snakegourd seeds prepared by the invention have the characteristics of complete nutrients, uniform taste, stable quality, delicate fragrance and easiness of cracking, and the biting rate of insects including mosquitoes, flies and the like can also be greatly reduced, so that the eating safety of the product is improved; furthermore, the snakegourd seeds have relatively good effects of invigorating the stomach and moistening the lung, maintaining beauty and keeping young and the like; people do not easily get internal heat after eating the seeds for a long period; and a preparation method of the insect-prevention crispy snakegourd seeds is simple and reasonable and a popularization value is very good.

The invention discloses a cryptomeria fortunei tissue culture rapid-propagation method. The method comprises taking a cryptomeria fortunei explant stem segment, inoculating the cryptomeria fortunei explant stem segment on an inducing adventitious bud culture medium which comprises DCR+ 0.1-2.0mg/L of 6-BA+ 0.1-2.0mg/L of IBA+ 0.5-5.0g/L of AC+ 0.001-0.01mg/L of TDZ+ 30g/L of cane sugar, and performing inducing culture to generate adventitious buds; adopting two stages to take root for rooting culture, namely first placing a rooting culture medium 1 which comprises 1/2 of DCR+ 0.1-2.0mg/L of NAA+ 0.1-2.0mg/L of IBA+0.1-1.0mg/L of 6-BA+ 1.0-10.0g/L of AC+ 10.0-30.0g/L of cane sugar, first performing dark culture for 2-15d, performing culture for 5-30d under illumination, and transferring to a rooting culture medium 2 which comprises DCR+ 0.1-1.0mg/L of NAA+ 0.1-1.0mg/L of 6-BA+ 0.5-5.0g/L of AC+ 30.0g/L of cane sugar. By the method, the tender bud propagation coefficient is over 5.7, and the rooting rate is over 80%. The culture cycle is short, the propagation quantity is large, and the cryptomeria fortunei tissue culture rapid-propagation method is favorable for large-scale production.

The invention relates to the field of wood processing, in particular to a hot-pressing drying method of cryptomeria fortunei wood. The hot-pressing drying method comprises the specific steps that thewater content of the cryptomeria fortunei wood is adjusted, and the processes of hot plate closed heating, hot plate opening, aftertreatment and the like are carried out. According to the hot-pressingdrying method, the wood can be dried to the required water content within short time, meanwhile, the cryptomeria fortunei wood can be compressed to a certain degree, the mechanical property of the cryptomeria fortunei wood is effectively improved, a processing technology is simple and feasible, the processing period is short, and operation is convenient; drying and enhancing of the cryptomeria fortunei wood are achieved at the same time, the problems of the low mechanical strength, drying and the like about the cryptomeria fortunei wood are solved, and the application of the cryptomeria fortunei wood is widened; and the cryptomeria fortunei wood can be used for the fields of furniture manufacturing, interior decoration and the like.

The invention belongs to the field of daily chemical new materials, and relates to a purely botanical negative ion spray. The product is prepared by mixing, by weight, 6.0-12.0 parts of pine needle extract, 10.0-15.0 parts of metasequoia extract, 10.0-13.0 parts of Platycladus orientalis extract, 6.0-12.0 parts of Taxodium ascendens extract, 8.0-12.0 parts of Cryptomeria fortunei extract, 12.0-18.0 parts of orange peel extract, 5.0-10.0 parts of lavender, 5.0-10.0 parts of mint extract, 5.0-10.0 parts of seaweed extract, 12.0-20.0 parts of grapefruit peel extract, 6.0-12.0 parts of green tea extract, 6.0-12.0 parts of camphor tree leaf extract, 10.0-18.0 parts of chitin extract, 5.0-10.0 parts of coconut shell extract, 0.0-15.0 parts of purified water and 1.0-2.0 parts of a light rare earth oxide catalyst. A production technology of the product adopts a leaching extraction method; and the extraction method is characterized in that plant stems and leaves are coarsely crushed, and then are leached with 45 DEG C hot water as a medium according to a solid/liquid ratio of 1:15 for 2 h twice to prepare every plant extract.

The invention discloses a cryptomeria fortunei CfCCR gene and an application thereof, belonging to the technical fields of plant molecular biology and gene engineering. The cryptomeria fortunei ligninsynthesis regulation CfCCR gene provided by the invention has a nucleotide sequence as shown in SEQ ID NO. 1, and the amino acid sequence of an expression protein of the cryptomeria fortunei lignin synthesis regulation CfCCR gene is as shown in SEQ ID NO. 2. The CfCCR gene is a key rate-limiting enzyme of a lignin synthesis specific pathway, regulates the expression of related genes for synthesizing lignin in plants through encoding protein, and plays an important role in biosynthesis and stress resistance of lignin. The tobacco transformed with the CfCCR gene is increased in lignin expression and developed in cell walls. Thus, the thickness of xylem cell walls in plant stems like cryptomeria fortunei can be changed by expressing the CfCCR gene, so the wood density is increased, and the wood quality and plant stress resistance are improved.

The invention provides a root salt control method for interplanting kaline soil corn and suaeda salsa. The root salt control method comprises the step of laying a percolation layer, wherein the percolation layer comprises rice straw biomass charcoal, cryptomeria wood dust, zeolite, crape myrtle bark and peanut press pulp. The method provided by the invention has the beneficial effects that the root salt control method for interplanting kaline soil corn and suaeda salsa provided by the invention method has a good improving effect on the physical and chemical properties of the soil in the kaline soil; after the three months, the soil volume weight of the 10 to 30cm soil layer under the corn plant is 1.15 to 1.22g/cm<3>; the soil volume weight of the 30 to 50cm soil layer under the corn plant is 1.13 to 1.20g/cm<3>; the salt content of the soil of the 10 to 30cm soil layer under the corn plant is 0.16 to 0.20 percent; the salt content of the soil of the 30 to 50cm soil layer under the corn plant is 0.14 to 0.19 percent.

The present invention discloses a cryptomeria fortunei CfICE1 gene and an application thereof, and belongs to the technical field of genetic engineering. A new ICE gene is cloned from cryptomeria fortunei tissues and named CfICE1. A nucleotide sequence is shown as SEQ ID NO.1. An amino acid sequence of an expressed protein is shown as SEQ ID NO.2. An expression vector PBI-CfICE1 is constructed totransform arabidopsis thaliana for functional verification and the CfICE1 is found to be over-expressed in the arabidopsis thaliana to promote increased cold resistance of the arabidopsis thaliana; and a qRT-PCR technology is used to analyze expression amount of the CfICE1 gene under different cryptomeria fortunei tissues and different low temperature stress, and the results show that the expression amounts of the CfICE1 are the highest in seeds and young leaves of cryptomeria fortunei at 4 DEG C. The above results indicate that the CfICE1 gene participates in growth and development of the cryptomeria fortunei and response to the low temperature stress, can be used in improvement of plant resistance genetic engineering, and has a broad application prospect.

The invention discloses a method for identifying the infection condition of the gall disease of cryptomeria fortunei. The method comprises the following steps: drying a proper amount of branches or needles of cryptomeria fortunei to be detected, grinding into powder, mixing the powder and potassium bromide, and tableting the mixture; collecting an infra-red spectrogram of the tablet; determining whether the cryptomeria fortunei to be detected is infected with gall disease or not according to the infrared spectrogram. According to the method, the difference between characteristic peaks of infrared spectrograms of healthy cryptomeria fortunei and cryptomeria fortunei infected with gall disease can be discovered by utilizing the Fourier transform infrared spectroscopy technology, and whether the cryptomeria fortunei is infected with pathogen of the gall disease or not can be detected by utilizing the difference, the detection process is non-destructive, quick, accurate and convenient to operate, is independent of human experience, has high safety and repeatability, is a new technical means provided to effectively prevent and control the gall disease of cryptomeria fortunei.

Disclosed is the fast tissue culturing and propagating technology for Taxodium mucronatum X Cryptomeria fortunei, wherein the basic cultivation medium of Taxodium mucronatum X Cryptomeria fortunei tissue cultivation comprises a large quantity of elements, ferric salts, organics, microelements and other substance, the fast tissue culturing and propagating method comprises explant sterilization, sterile sprout establishment, test tube sprout breeding, root culturing and transplanting.

The invention discloses a method for rapidly identifying and evaluating cold resistance of cryptomeria fortunei, and belongs to the technical field of crop science. The method mainly comprises the following steps: (1) carrying out cutting propagation of a clone of cryptomeria fortunei to be detected; (2) performing low-temperature treatment on the cryptomeria fortunei cutting seedlings; (3) measuring the change conditions of cell membrane permeability, semi-lethal temperature, malondialdehyde, SOD and soluble protein content of the low-temperature stressed cryptomeria fortunei leaves; (4) evaluating the cold resistance so as to determine the strength of the cold resistance of the cryptomeria fortunei to be detected; and (5) carrying out cold resistance verification and further screening. According to the method, on the basis that the cold resistance of the cryptomeria fortunei is comprehensively evaluated, it is found that the cold resistance of more than 80% of the clone of the cryptomeria fortunei can be quickly judged without damage by enabling clonal in-vitro lateral branches of cryptomeria fortunei to be subjected to low-temperature stress at -4 DEG C and measuring the relative conductivity, so that a method for rapidly identifying and evaluating the cold resistance of the cryptomeria fortunei is further provided, and the method has very important significance for reasonable planning and layout of utilization, planting and the like of cryptomeria fortunei germplasm resources and cold resistance breeding.

The present invention provides nucleic acid sequences coding for the Cryptomeria japonica major pollen allergen Cry j I, Cry j II, Jun s I and Jun v I and fragments or peptides thereof. The present invention also provides purified Cry j I, Cry j II, Jun s I and Jun v I and at least one fragment thereof produced in a host cell transformed with a nucleic acid sequence coding for Cry j I, Cry j II, Jun s I and Jun v I or at least one fragment thereof, and fragments of Cry j I, Cry j II, Jun s I or Jun v I or at least one fragment thereof, and fragments of Cry j I, Cry j II, Jun s I or Jun v I prepared synthetically. Cry j I, Cry j II, Jun s I and Jun v I and fragments thereof are useful for diagnosing, treating, and preventing Japanese cedar pollinosis. The present invention also provides isolated peptides of Cry j I and Cry j II. Peptides within the scope of the invention comprise at least one T cell epitope, or preferably at least two T cell epitopes of Cry j I or Cry j II. The invention also pertains to modified peptides having similar or enhanced therapeutic properties as the corresponding naturally-occurring allergen or portion thereof but having reduced side effects. Methods of treatment or of diagnosis of sensitivity to Japanese cedar pollens in an individual and therapeutic compositions, and multipeptide formulations comprising one or more peptides of the invention are also provided.

The invention discloses a method for extracting T-Cadinol from Cryptomeria japonica. The method comprises steps of: crushing leaves of Cryptomeria japonica; carrying out supercritical CO2 extraction to obtain an extract; adding active carbon for decolorization; enriching an obtained decolorization solution by a polyamide resin column chromatography; condensing the destaining solution and then extracting the destaining solution with ether; and drying to obtain a T-Cadinol extractive. The preparation method has high yield and is suitable for industrialized production.

The invention discloses fluorescent quantitative reference genes of different tissue of Cryptomeria fortunei as well as special primers and application thereof, and belongs to the technical field of plant molecular biology. The fluorescent quantitative reference gene is a UBI gene, a CYP gene and an SDH1 gene, and the nucleotide sequences of the UBI gene, the CYP gene and the SDH1 gene are respectively shown as SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3. Twelve candidate reference gene sequences are screened through cryptomeria fortunei transcriptome data, the stability of the candidate reference genes is evaluated by using three reference gene stability evaluation software, and the optimal genes UBI, CYP and SDH1 for real-time fluorescent quantitative PCR of different tissue of cryptomeria fortunei are obtained. And a real-time fluorescent quantitative PCR primer of the reference gene is designed, and the primer is high in specificity and amplification efficiency, so that the detection efficiency of detecting the cryptomeria fortunei gene in a real-time fluorescent quantitative manner can be greatly improved, and the credibility of a detection result is improved.

A composition utilizing natural materials is selected from a group of plant extract comprising: Taiwan cypress, stout camphor tree, Taiwan incense cedar, Cinnamomum camphora, Cymbopogon nardus, Litsea cubeba, Cinnamomum zeylanicum, Taiwan Cunninghamia lanceolatavar, Melaleuca alternifolia, Eucalyptus robusta smith, Cryptomeria japonica and Acacia confuse. The tumor cell-inhibited composition having the efficiency of inhibiting the growth of tumor cells is formed by mixing these extracts based on a specific ratio.

The invention belongs to the technical field of cedar wood processing, and in particular relates to a processing method for improving the properties of cedar wood, which comprises several steps of plate pretreatment, sol dry powder preparation, plate treatment and hot-press forming. Compared with the prior art, the present invention has the following advantages: in the present invention, the board is first controlled to a certain water content, and then the dry powder of the sol is brought into the capillary system and the pit system of the board by using high-temperature carbon dioxide, so as to improve the hardness of the board and at the same time improve the hardness of the board. Antibacterial and anti-corrosion properties, the sol dry powder forms regular nanoparticles in the board, which is uniformly dispersed, not easy to agglomerate, and has a stable structure, which effectively improves the dimensional stability of the board. At the same time, the nail-holding force of the board will not decrease, and the processing process is simple. The dry quality of fir improves the compactness of the board and effectively expands the scope of use of the product.

The invention relates to the technical field of composite materials, and specifically relates to a flame-retardant neosinocalamus affinis and cryptomeria fortunei composite board manufacturing method. The manufacturing method comprises the steps of neosinocalamus affinis sawing, cryptomeria fortunei sawing, material flame retardant treatment, glue mixing, material selection and sizing, standing and assembly, pre-pressing and hot-pressing, and cooling, edge cutting and modification. The neosinocalamus affinis using range is extended, and single-purpose performance of the neosinocalamus affinis as pulp production materials and caused environmental pollution are prevented. The board produced by the method is integrated with low-cost characteristic of the cryptomeria fortunei and high toughness characteristic of the neosinocalamus affinis, and has good mechanical property. In addition, the board produced by the method has good flame retardant property, and the method and basis are provided for production of flame-retardant wood substrate composite materials.

A production method of a Cryptomeria fortune timber homogeneous carbonized wood comprises eleven operations: cutting, degreasing, drying, moisture regain, stacking, feeding, heating, carbonization, cooling and drawing. The invention has the following beneficial effects: the Cryptomeria fortune timber is subjected to a degreasing treatment first to lay a good foundation for the realization of homogeneous carbonization; for feeding, the timer is coated with kaolin, so as to completely isolate from the air and uniformize carbonization effect; and after kiln sealing, the carbonization temperature is reduced overall, so that the same degree of carbonization on the Cryptomeria fortunei timber from the outer to the inner parts can be guarantees, and the moisture absorption, dimensional stability, mechanical properties, corrosion resistance, hardness, compressive strength, bonding performance, coating performance, especially color of all parts of the timber show the same good characteristics. By improving the degreasing method, carbonization auxiliary materials and carbonization temperature and time, the carbonized wood can reach better homogenization effect; and the bonding effect can improved, and application value and economic benefit of the product can be increased.