A method to promote somatic embryogenesis and maturation of Cryptomeria japonica

By inducing embryogenic callus and somatic embryogenesis through specific culture media and procedures, the problem of low somatic embryogenesis efficiency in Chinese fir was solved, enabling rapid propagation of superior genetic resources and stable transmission of genetic traits, thus promoting the breeding process of superior Chinese fir varieties.

CN120323326BActive Publication Date: 2026-05-05NANJING FORESTRY UNIV
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Patent Information

Application Number
CN202510674500.1
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2025-05-23
Publication Date
2026-05-05
Estimated Expiration
2045-05-23

AI Technical Summary

Technical Problem

The somatic embryogenesis system of Chinese fir is imperfect, with low embryogenesis efficiency, low maturity rate, and a high proportion of deformed embryos, which limits its application in genetic improvement and breeding of superior varieties.

Method used

Using a specific culture medium and procedures, embryogenic callus was induced from immature seed embryos of superior cedar families as explants, and then cultured for proliferation. The callus was then transferred to a somatic embryo induction medium to obtain somatic embryos, which were then matured in a somatic embryo maturation medium to finally obtain regenerated plants.

Benefits of technology

An efficient system for inducing embryogenic callus and somatic cell development in Cryptomeria japonica was established, enabling rapid propagation of superior genetic resources, improving somatic embryo maturation rate and breeding efficiency, and ensuring the stability of genetic traits in offspring plants.

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Abstract

This invention discloses a method for promoting somatic embryogenesis and maturation in Cryptomeria japonica, belonging to the field of somatic embryogenesis technology. The method involves using immature seed embryos as explants to induce embryogenic callus; transferring the proliferated embryogenic callus to a somatic embryogenesis induction medium for further culture to obtain somatic embryos; transferring the somatic embryos to a somatic embryogenesis maturation medium for dark culture to obtain mature cotyledonary embryos; transferring the mature cotyledonary embryos to a somatic embryogenesis basal medium for light culture to obtain somatic embryonic seedlings; and hardening off the seedlings before transplanting to obtain regenerated plants. The results of this application show that embryogenic callus can be stably obtained from different families, with high somatic embryogenesis maturation capacity and rate, enabling the cultivation of a large number of high-quality seedlings with stable genetic traits in a short time; avoiding the phenotypic segregation problem caused by gene recombination during seed propagation, and ensuring that offspring plants inherit the superior characteristics of the parents.
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