Bacillus cereus complex culture tank

Abstract

The utility model relates to composite culture jar technical field especially relates to bacillus cereus composite culture jar, including support platform and transparent incubation jar, the upper end of support platform is equipped with the transparent incubation jar for cultivating bacillus cereus and being convenient for observation.

Description

Technical Field

[0001] This utility model relates to the field of composite culture tank technology, and in particular to a composite culture tank for Bacillus cereus. Background Technology

[0002] Bacillus cereus is a Gram-positive, aerobic or facultative anaerobic spore-forming bacillus widely found in soil, dust, grains, and other agricultural products. It is an opportunistic pathogen capable of causing diseases in humans and animals under specific conditions. In the cultivation of Bacillus cereus, composite culture tanks are required for culturing Bacillus cereus and its compound preparations. These tanks are widely used in the field of microbial fermentation, and their structure and function are designed to provide optimal conditions for the growth and reproduction of microorganisms.

[0003] Meanwhile, in existing composite culture tanks, the air inlet and outlet positions may be improperly installed, resulting in uneven gas distribution. Fresh air flows directly from the air inlet to the outlet without being fully mixed with the culture medium, which limits the cultivation and growth of microorganisms and reduces the overall cultivation effect. Utility Model Content

[0004] To overcome the problem that improper installation of the air inlet and exhaust port of existing composite culture tanks may lead to uneven gas distribution, with fresh air flowing directly from the air inlet to the exhaust port without being fully mixed with the culture medium, thus limiting the cultivation and growth of microorganisms and reducing the overall culture effect, this utility model provides a Bacillus cereus composite culture tank.

[0005] The technical solution is as follows: A Bacillus cereus composite culture jar includes a support platform and a transparent culture jar; the upper end of the support platform is equipped with a transparent culture jar for culturing Bacillus cereus and facilitating observation; it also includes a placement tray, an exhaust pipe, a rubber sealing head, an air inlet pipe, a cover plate, a support frame, a weight block, and a heating plate; several sets of placement trays are installed inside the transparent culture jar, and two sets of rubber sealing heads are installed inside the placement trays; multiple sets of exhaust pipes adapted to and connected to the placement trays are installed at the outer end of the transparent culture jar; multiple sets of air inlet pipes are installed on the outer wall of the transparent culture jar away from the exhaust pipes; multiple sets of air inlet pipes and exhaust pipes all extend through the transparent culture jar to the inside of the rubber sealing head; the rubber sealing head is fitted with the air storage tank and the air inlet pipe; multiple sets of air inlet pipes and exhaust pipes are connected to the placement tray; electromagnetic valves are installed at the outer ends of multiple sets of air inlet pipes and exhaust pipes; and a docking plate is fixed to one end of each air inlet pipe and exhaust pipe.

[0006] Furthermore, a support frame is installed at the lower end of the support platform, and a drive rod is provided inside the support frame. One end of the drive rod is located at the lower end of the support frame and a drive motor is fixed thereon. The drive rod is fixed to the output end of the drive motor and the drive rod is fixedly connected to the support platform. A load-bearing column is fixed circumferentially at the lower end of the support frame.

[0007] Furthermore, a weight block is installed at the lower end of the support plate on one side of the drive rod, and a positioning block is installed between the weight block and the support plate. Two sets of limiting grooves for accommodating the positioning blocks are opened at the bottom of the support plate. The weight block is fixed to the support plate by inserting the positioning blocks, and a movable rod is installed between the support plate and the drive rod.

[0008] Furthermore, the lower end of the transparent culture tank is fixed with a connector, and the upper end of the support plate is provided with multiple positioning grooves to accommodate the connector. The transparent culture tank is fixed to the support plate by inserting the connector.

[0009] Furthermore, several sets of limiting pins are installed circumferentially on the outer end of the transparent culture tank, and several sets of placement trays have connecting holes inside to accommodate the limiting pins. All sets of placement trays are fixed to the transparent culture tank by inserting the limiting pins.

[0010] Furthermore, a sealing cap is fastened to the upper end of the transparent culture tank, and two sets of pull rods are fixed to the upper end of the sealing cap.

[0011] Furthermore, the upper ends of the multiple placement trays are fastened with cover plates, and a connector is provided above the cover plates. Multiple connecting rods are installed between the connector and the cover plates.

[0012] Furthermore, a control module is installed on the outer end of the support frame, and a heating plate is installed on the lower inner end of the transparent incubator. Multiple sets of solenoid valves and the heating plate are electrically connected to the control module.

[0013] The beneficial effects are: This utility model achieves precise gas exchange by using electromagnetic valves to connect the exhaust pipe and the intake pipe respectively, controlling the flow rate of gas intake and exhaust, and adjusting the gas exchange volume in real time according to the oxygen demand of microorganisms during the cultivation process, ensuring that the oxygen supply in the cultivation tank is always in the optimal state, avoiding insufficient oxygen supply that restricts the cultivation and growth of microorganisms, improving the cultivation effect, and the sufficient oxygen supply optimizes the microbial growth environment, assisting its rapid growth and reproduction, and improving the overall fermentation efficiency. Attached Figure Description

[0014] Figure 1 This is a three-dimensional structural diagram of the Bacillus cereus composite culture tank of this utility model;

[0015] Figure 2 This is a three-dimensional structural diagram of the transparent culture tank of this utility model;

[0016] Figure 3 This is a three-dimensional structural diagram of the placement tray of this utility model;

[0017] Figure 4 This is a three-dimensional structural diagram of the support frame of this utility model;

[0018] Figure 5This is a schematic diagram of the three-dimensional structure of the weight block of this utility model.

[0019] In the attached diagram, the following are the reference numerals: 1. Support plate; 2. Transparent incubator; 3. Placement tray; 4. Exhaust pipe; 5. Solenoid valve; 6. Connecting plate; 7. Rubber sealing head; 8. Air inlet pipe; 9. Cover plate; 10. Connector; 11. Limit pin; 12. Sealing cover; 13. Pull rod; 14. Support frame; 15. Weight block; 16. Drive rod; 17. Drive motor; 18. Connector; 19. Load-bearing column; 20. Control module; 21. Heating plate. Detailed Implementation

[0020] The present invention will now be described in detail with reference to the accompanying drawings and specific embodiments.

[0021] Bacillus cereus is a Gram-positive, aerobic or facultative anaerobic rod-shaped bacterium widely found in soil, water, air, and various foods. Its cell morphology is typically short or long rod-shaped, approximately 1-2 μm × 3-5 μm in size, possessing peritrichous flagella, making it motile. The spores are oval, located centrally or slightly off-center, and the sporangia do not swell. On nutrient agar, Bacillus cereus colonies are grayish-white or slightly yellow, with a rough, dry surface, irregular edges, and a diameter generally around 2-5 mm.

[0022] This bacterium has a wide growth temperature range, generally between 10℃ and 45℃, with an optimal growth temperature of approximately 30℃-35℃ and an optimal pH of 6.0-7.5. It can produce various enzymes, such as proteases, amylases, and lipases, which play important roles in food spoilage. Bacillus cereus also exhibits strong heat resistance; its spores can survive boiling in 100℃ water for several minutes, which means it may remain in some heat-processed foods.

[0023] In terms of pathogenicity, Bacillus cereus primarily causes food poisoning, and its pathogenic mechanism mainly involves the production of enterotoxins. There are two main types of enterotoxins produced by this bacterium: vomiting enterotoxin and diarrheal enterotoxin. Vomiting enterotoxin is a heat-stable protein toxin, which is not easily destroyed even under boiling conditions. After ingesting food containing this toxin, the incubation period is short, generally within 1-6 hours, with main symptoms being nausea and vomiting, usually without diarrhea. The course of the illness is short, and recovery usually occurs spontaneously within 24 hours. Diarrheal enterotoxin, on the other hand, is a heat-labile protein toxin, which is unstable to heat and easily destroyed by heating. Its incubation period is relatively long, generally 8-16 hours, with main symptoms being diarrhea and abdominal pain, which may be accompanied by nausea and vomiting. The course of the illness is generally 1-3 days.

[0024] Food poisoning caused by Bacillus cereus occurs worldwide, especially in areas with poor sanitation and improper food processing and storage. Commonly contaminated foods include leftovers, cold dishes, cooked meat products, dairy products, and soy products. The key to preventing Bacillus cereus food poisoning lies in strengthening food hygiene management and strictly controlling the processing, storage, and sale of food. For example, maintaining good personal and environmental hygiene during food processing is crucial to prevent food contamination; food should be thoroughly cooked, especially leftovers; storage temperature and time should be controlled to minimize opportunities for bacterial growth; and supervision of food production and processing enterprises should be strengthened to ensure that food meets hygiene standards.

[0025] A Bacillus cereus composite culture vessel is a device specifically designed for culturing Bacillus cereus, providing suitable environmental conditions for its growth and reproduction. These vessels typically possess multiple functions, such as precise control of parameters like temperature, pH, and dissolved oxygen during the culture process to meet the optimal growth conditions for Bacillus cereus. For instance, the optimal growth temperature range for Bacillus cereus is 20°C to 40°C, commonly 37°C; the culture vessel can stabilize the temperature within this range, thus ensuring rapid bacterial growth.

[0026] In addition, composite culture tanks may be equipped with stirring devices to ensure that nutrients in the culture medium are evenly distributed, promoting bacterial absorption of nutrients. Regarding technical parameters, the capacity of the culture tanks can be selected according to actual needs, ranging from a few liters for small-scale laboratory operations to hundreds of liters or even larger for industrial production. Meanwhile, the materials used in the culture tanks are usually chosen to be corrosion-resistant and easy-to-clean, such as stainless steel, to ensure the lifespan of the equipment and the hygiene of the culture environment.

[0027] In practical applications, Bacillus cereus multi-culture tanks are widely used in microbiology research, biopharmaceuticals, agriculture, and other fields. For example, in agriculture, Bacillus cereus can be used as a bio-fertilizer to promote plant growth, and multi-culture tanks can cultivate this bacterium in large quantities, providing sufficient inoculum for agricultural production. In the biopharmaceutical field, Bacillus cereus can be used to produce some bioactive substances, and multi-culture tanks can be used to optimize the fermentation process and increase yield.

[0028] like Figures 1-5As shown, the Bacillus cereus composite culture jar includes a support platform 1 and a transparent culture jar 2. The upper end of the support platform 1 is fitted with the transparent culture jar 2 for culturing Bacillus cereus while facilitating observation. It also includes a placement tray 3, an exhaust pipe 4, a rubber sealing head 7, an air inlet pipe 8, a cover plate 9, a support frame 14, a weight block 15, and a heating plate 21. Several sets of placement trays 3 are installed inside the transparent culture jar 2, and two sets of rubber sealing heads 7 are installed inside each set of placement trays 3. Multiple sets of exhaust pipes 4, adapted to and connected to the placement trays 3, are installed at the outer end of the transparent culture jar 2. Multiple sets of air inlet pipes 8 are installed on the outer wall of the transparent culture jar 2 away from the exhaust pipes 4. The multiple sets of air inlet pipes 8 and the exhaust pipes... All 4 extend through the transparent culture tank 2 to the inside of the rubber sealing head 7. The rubber sealing head 7 is fitted with the air storage tank and the air inlet pipe 8. Multiple sets of air inlet pipes 8 and exhaust pipes 4 are connected to the placement tray 3. Solenoid valves 5 are installed at the outer ends of multiple sets of air inlet pipes 8 and exhaust pipes 4. A docking plate 6 is fixed to one end of the air inlet pipe 8 and the exhaust pipe 4. A support frame 14 is installed at the lower end of the support platform 1. A drive rod 16 is provided inside the support frame 14. A drive motor 17 is fixed at one end of the drive rod 16 at the lower end of the support frame 14. The drive rod 16 is installed and fixed to the output end of the drive motor 17. The drive rod 16 is fixedly connected to the support platform 1. A load-bearing column 19 is fixed circumferentially at the lower end of the support frame 14.

[0029] Please see Figures 2-4 A weight block 15 is installed on the lower end of the support plate 1, located on one side of the drive rod 16. A positioning block is installed between the weight block 15 and the support plate 1. Two sets of limiting grooves for accommodating the positioning blocks are opened at the bottom of the support plate 1. The weight block 15 is fixed to the support plate 1 by inserting it into the positioning block. A movable rod is installed between the support plate 1 and the drive rod 16. A connector 18 is fixed at the lower end of the transparent culture tank 2. Multiple sets of positioning grooves for accommodating the connector 18 are opened at the upper end of the support plate 1. The transparent culture tank 2 is fixed to the support plate 1 by inserting it into the connector 18. Several sets of limiting pins 11 are installed circumferentially on the outer end of the transparent culture tank 2. Several sets of placement trays 3 have connecting holes for accommodating the limiting pins 11 inside. Several sets of placement trays 3 are all fixed to the transparent culture tank 2 by inserting them into the limiting pins 11.

[0030] Please see Figures 3-5 The upper end of the transparent culture tank 2 is fitted with a sealing cover 12. Two sets of pull rods 13 are fixed to the upper end of the sealing cover 12. The upper end of the multiple placement trays 3 is fitted with a cover plate 9. A connector 10 is provided above the cover plate 9. Multiple sets of connecting rods are installed between the connector 10 and the cover plate 9. A control module 20 is installed on the outer end of the support frame 14. A heating plate 21 is installed on the lower end of the interior of the transparent culture tank 2. Multiple sets of solenoid valves 5 and the heating plate 21 are electrically connected to the control module 20.

[0031] When culturing Bacillus cereus, the desired culture is first injected into the placement tray 3. After injecting the culture into a single placement tray 3, the cover tray 9 is fastened and sealed to the placement tray 3. After injecting the culture into the placement trays 3 sequentially, the placement trays 3 are placed into the transparent culture tank 2. The placement trays 3 and transparent culture tank 2 are fixed together using the limiting pin 11. After the placement trays 3 are fixed in place, the connecting rod 13 is manually grasped to connect the sealing cover 12 to the transparent culture tank 2 for fastening and sealing. Next, the exhaust pipe 4 and the air inlet pipe 8 required for culturing the culture are inserted into the transparent culture tank 2 and connected to the rubber sealing head 7, so that the exhaust pipe 4 and the air inlet pipe are aligned. 8 is connected to the placement tray 3. After the transparent culture tank is placed, the positioning block is used to assist the load-bearing block in fixing the bottom of the support plate 1. After the load-bearing block is installed, one side of the support plate 1 is heavier than the other side. The movable rod can assist the support plate 1 in tilting along the upper end of the drive rod 16. Next, the connector 18 is used to insert and fix the transparent culture tank 2 into the positioning groove near the edge of the upper end of the support plate 1. At the same time, the drive motor 17 is started and connected to the drive rod 16 to drive the support plate 1 to tilt and rotate, stirring the culture medium injected into the placement tray 3, so that the nutrients are evenly distributed and the microbial growth is promoted.

[0032] After the drive motor 17 is used to stir and mix the cultured bacteria, the weight block 15 is removed, and the transparent culture tank 2 is installed into the positioning groove at the center of the upper end of the support plate 1 using the connector 18 to maintain vertical culture. When air exchange is required during the culture process, the control module 20 controls the solenoid valve 5 to open the exhaust pipe 4 and the air inlet pipe 8 respectively to carry out precise gas exchange, control the air intake and exhaust flow rate, and adjust the gas exchange volume in real time according to the oxygen demand of the microorganisms during the culture process to ensure that the oxygen supply in the culture tank is always in the optimal state, avoid insufficient oxygen supply to limit the culture and growth of microorganisms, and optimize the microbial growth environment, assisting their rapid growth and reproduction. At the same time, when the internal temperature of the transparent culture tank 2 is low, the control module 20 is used to turn on the heating plate 21 to heat the inside of the transparent culture tank 2 to ensure the internal culture temperature balance and achieve stable culture of the bacteria.

[0033] The above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A complex culture tank of Bacillus cereus, characterized by, It includes a support platform (1) and a transparent culture tank (2); the upper end of the support platform (1) is equipped with a transparent culture tank (2) for culturing Bacillus cereus and facilitating observation; it also includes a placement tray (3), an exhaust pipe (4), a rubber sealing head (7), an air inlet pipe (8), a cover plate (9), a support frame (14), a weight block (15), and a heating plate (21); several sets of placement trays (3) are installed inside the transparent culture tank (2), and two sets of rubber sealing heads (7) are installed inside the several sets of placement trays (3); multiple sets of rubber sealing heads (7) are installed at the outer end of the transparent culture tank (2). 3) The exhaust pipe (4) is adapted to be connected. Multiple sets of air inlet pipes (8) are installed on the outer wall of the transparent culture tank (2) away from the exhaust pipe (4). The multiple sets of air inlet pipes (8) and exhaust pipes (4) all pass through the transparent culture tank (2) and extend into the rubber sealing head (7). The rubber sealing head (7) is fitted with the gas storage tank and the air inlet pipe (8). The multiple sets of air inlet pipes (8) and exhaust pipes (4) are connected to the placement tray (3). The outer ends of the multiple sets of air inlet pipes (8) and exhaust pipes (4) are all equipped with electromagnetic valves (5). One end of the air inlet pipe (8) and the exhaust pipe (4) is fixed with a docking plate (6).

2. The B. cereus complex culture tank according to claim 1, characterized by A support frame (14) is installed at the lower end of the support platform (1). A drive rod (16) is provided inside the support frame (14). A drive motor (17) is fixed at one end of the drive rod (16) at the lower end of the support frame (14). The drive rod (16) is fixed to the output end of the drive motor (17). The drive rod (16) is fixedly connected to the support platform (1). A load-bearing column (19) is fixed around the lower end of the support frame (14).

3. The B. cereus complex culture tank according to claim 2, characterized in that, A weight block (15) is installed at the lower end of the support plate (1) on one side of the drive rod (16). A positioning block is installed between the weight block (15) and the support plate (1). Two sets of limiting grooves for accommodating the positioning blocks are opened at the bottom of the support plate (1). The weight block (15) is fixed to the support plate (1) by the positioning block. A movable rod is installed between the support plate (1) and the drive rod (16).

4. The B. cereus complex culture tank according to claim 1, characterized by The lower end of the transparent culture tank (2) is fixed with a connector (18), and the upper end of the support plate (1) is provided with multiple positioning grooves to accommodate the connector (18). The transparent culture tank (2) is fixed to the support plate (1) by the connector (18).

5. The B. cereus complex culture tank according to claim 4, characterized in that, Several sets of limiting pins (11) are installed circumferentially on the outer end of the transparent culture tank (2). Several sets of placement trays (3) have connection holes for accommodating the limiting pins (11) inside. Several sets of placement trays (3) are all connected and fixed to the transparent culture tank (2) by the limiting pins (11).

6. The B. cereus complex culture tank according to claim 5, characterized in that The upper end of the transparent culture tank (2) is fitted with a sealing cover (12), and the upper end of the sealing cover (12) is fixed with two sets of pull rods (13).

7. The B. cereus complex culture tank according to claim 6, characterized in that The upper end of the multiple placement trays (3) is fastened with a cover plate (9), and a connector (10) is provided above the cover plate (9). Multiple connecting rods are installed between the connector (10) and the cover plate (9).

8. The B. cereus complex culture tank according to claim 7, characterized in that, A control module (20) is installed on the outer end of the support frame (14), and a heating plate (21) is installed on the lower inner end of the transparent incubator (2). Multiple sets of solenoid valves (5) and the heating plate (21) are electrically connected to the control module (20).

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