A microbial separation device for pharmaceutical microbiological testing
By combining centrifugal separation with a filtration membrane layer and using a multi-layer filtration and adsorption assembly, the problem of low efficiency in traditional microbial separation is solved, achieving rapid and effective microbial separation and excellent control of the growth environment.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- YICHUN WUJIASHEN PHARM CO LTD
- Filing Date
- 2025-06-26
- Publication Date
- 2026-06-23
AI Technical Summary
Traditional microbial isolation methods have low separation efficiency, making it difficult to separate different types of microorganisms. They are also susceptible to external environmental pollution, with impurities having a significant impact on the separation process.
By combining centrifugal separation with filtration membranes, microorganisms are rapidly separated through filtration membranes with different pore sizes. This is combined with multi-layer filtration and adsorption components to remove impurities and provide a constant-temperature growth environment.
It enables rapid and effective microbial isolation, improves testing efficiency, ensures a pure microbial growth environment, avoids interference from impurities, and shortens the separation time.
Smart Images
Figure CN224394868U_ABST
Abstract
Description
Technical Field
[0001] This application relates to the field of pharmaceutical microbiology testing technology, and more specifically, to a microbial separation device for pharmaceutical microbiology testing. Background Technology
[0002] The main purpose of pharmaceutical microbiological testing is to ensure that drugs are not contaminated by microorganisms during production, storage, and use, thereby ensuring patient safety. In the process of pharmaceutical microbiological testing, centrifugation devices are needed to effectively separate microorganisms from drug samples for subsequent detection and analysis. Traditional microbial separation methods usually have low separation efficiency. After centrifugation, the separated microorganisms need to be taken out and cultured in petri dishes for growth. This process is easily affected by external environmental contamination and is difficult to achieve the purpose of separating different types of microorganisms. In view of this, we propose a microbial separation device for pharmaceutical microbiological testing. Utility Model Content
[0003] 1. Technical problems to be solved
[0004] The purpose of this application is to provide a microbial separation device for pharmaceutical microbial testing, which solves the technical problems in the background art. The device has a reasonable overall layout and simple operation. By combining centrifugal separation with filtration membrane interception, and based on the different pore sizes of the filtration membrane, it can quickly and effectively separate different types of microorganisms from pharmaceutical samples, greatly shortening the separation time and improving the testing efficiency. The multi-layer filtration and adsorption components can not only effectively intercept microorganisms, but also remove impurities and harmful substances from the sample. It can also conveniently provide culture medium to the filtration membrane, allowing microorganisms to grow in a relatively sealed and constant-temperature environment inside the shell, located at the filtration membrane, ensuring an excellent microbial growth environment and avoiding the influence of impurities.
[0005] 2. Technical Solution
[0006] This application provides a microbial isolation device for pharmaceutical microbial testing, comprising: an outer shell, an end cap detachably and sealingly installed on the upper end of the outer shell, a storage tank and a controller detachably installed on the outside of the outer shell, a flower-shaped frame fixedly installed on the upper inner end of the outer shell by bolts, a centrifugal motor fixedly installed on the flower-shaped frame, a turntable drivenly connected to the output shaft of the centrifugal motor, multiple conical containers and culture vessels installed along the circumference of the turntable, the conical containers and culture vessels being arranged one-to-one, and the culture vessels being located at the bottom of the conical containers, a multi-layer filtration and adsorption assembly being detachably installed at the bottom of the culture vessels, the controller being provided with a constant temperature heating device, and an annular heating frame connected inside the outer shell, and a culture medium dispensing and conveying mechanism connected inside the storage tank for dispensing culture medium solution into each conical container;
[0007] The multi-layer filtration and adsorption assembly consists of a filter membrane layer and an adsorption layer arranged sequentially from top to bottom. The adsorption layer includes an activated carbon adsorption layer, a silica gel adsorption layer, and an antibacterial adsorption layer.
[0008] By adopting the above technical solution, when using this separation device, the microbial sample of the drug to be tested is pre-added into multiple conical containers located on a rotating disc. Each conical container has a corresponding incubator installed at its bottom. The end caps on the upper part of the outer shell are then sealed and closed. The centrifugal motor is started electrically via a controller. The output shaft of the centrifugal motor drives the rotating disc and multiple conical containers to rotate at high speed. Due to centrifugal force, the microorganisms in the sample are thrown out from the bottom of the conical containers onto the filter membrane, achieving preliminary separation of microorganisms from other impurities. Furthermore, based on the different pore sizes of the filter membrane, different types of microorganisms are retained by different filter membranes, achieving the purpose of separating different microorganisms. This is achieved by setting multiple... The controller regulates the culture medium dispensing and delivery mechanism, allowing the appropriate amount of culture medium solution from the storage tank to be injected into multiple conical containers. The culture medium solution provides a growth environment for microorganisms by passing through the filter membrane. During the cultivation process, the filter membrane prevents microorganisms from passing through, while various nutrients in the culture medium can be absorbed by the microorganisms through the filter membrane. The activated carbon adsorption layer, silica gel adsorption layer, and antibacterial adsorption layer play an adsorption role, removing impurities and harmful substances from the sample. Furthermore, the controller's built-in constant temperature heating device can maintain the internal temperature environment of the shell at the required conditions using a ring heating rack, ensuring the purity of the microbial growth environment, facilitating subsequent observation and detection, and avoiding interference from impurities, thereby meeting the needs of microbial growth.
[0009] Optionally, four pillars are fixedly provided at the bottom of the housing, and a drain valve pipe is fixedly connected to the bottom of the housing.
[0010] By adopting the above technical solution, the outer shell is made of transparent polycarbonate material, which gives the outer shell a certain strength and corrosion resistance. By setting the transparent structure, it is easy to observe the internal situation.
[0011] Optionally, two mounting bases are fixedly provided on the outside of the housing, and the storage tank and the controller are respectively fixedly mounted on one mounting base by bolts.
[0012] By adopting the above technical solution, the storage tank and controller are installed on the outside of the shell by mounting brackets. The storage tank is sealed and stored with culture medium solution, which is used to provide culture medium solution to the filter membrane layer. The controller can be used to regulate the centrifuge motor and provide centrifugation speed and time parameters for different microorganisms.
[0013] Optionally, the upper end of the storage tank is detachably and sealed with a cap. The culture medium dispensing and conveying mechanism includes a culture medium pump, which is fixedly installed on the flower-shaped frame by bolts. A suction pipe is detachably connected between the culture medium pump and the storage tank. The culture medium pump is fixedly connected to a delivery pipe. The bottom end of the delivery pipe is fixedly connected to an annular distribution pipe. Multiple outlet pipes are fixed along the circumferential thread of the annular distribution pipe and are arranged one-to-one with the conical container.
[0014] By adopting the above technical solution, when it is necessary to provide culture medium solution to the filter membrane layer, the centrifugal motor is paused and the conical containers are respectively stopped at the bottom of the corresponding outlet pipes. The culture medium pump is started by the controller to draw culture medium solution from the storage tank. The culture medium solution is delivered to the corresponding conical containers through multiple outlet pipes connected by the ring distribution pipe. The culture medium solution passes through the conical containers and permeates into the filter membrane layer, providing a good growth environment for microbial growth.
[0015] Optionally, a connecting sleeve is fixedly provided at the center of the turntable, and the output shaft of the centrifugal motor is movably inserted into the connecting sleeve, with two positioning bolts threaded in place.
[0016] By adopting the above technical solution, the output shaft of the centrifugal motor and the connecting sleeve are fixedly connected by two positioning bolts, so that the output shaft of the centrifugal motor can drive the turntable to rotate centrifugally inside the outer shell.
[0017] Optionally, the conical bottom of the conical container is uniformly provided with a plurality of micropores, and the diameter of the micropores is set between 0.1 and 1 micrometer.
[0018] By adopting the above technical solution, the conical bottom of the conical container is provided with micropores, which allow microorganisms and culture medium to pass through the micropores and be thrown onto the filter membrane layer.
[0019] 3. Beneficial effects
[0020] The technical solutions provided in this application, including one or more, have at least the following technical effects or advantages: The device is reasonably designed and easy to operate. By combining centrifugal separation with filtration membrane interception, and based on the different pore sizes of the filtration membrane, it can quickly and effectively separate different types of microorganisms from drug samples, greatly shortening the separation time and improving the testing efficiency. The multi-layer filtration and adsorption components can not only effectively intercept microorganisms but also remove impurities and harmful substances from the samples. It can also conveniently provide culture medium to the filtration membrane, allowing microorganisms to grow in a relatively sealed and constant-temperature environment inside the shell, located at the filtration membrane, ensuring an excellent microbial growth environment and avoiding the influence of impurities. Attached Figure Description
[0021] Figure 1 This is a schematic diagram of the overall structure of a microbial separation device for pharmaceutical microbial testing, as disclosed in a preferred embodiment of this application.
[0022] Figure 2 This is a schematic cross-sectional view of the outer shell of a microbial separation device for pharmaceutical microbial testing, as disclosed in a preferred embodiment of this application.
[0023] Figure 3 This is a schematic diagram of the culture medium dispensing and conveying mechanism, turntable, and culture vessel structure of a microbial isolation device for pharmaceutical microbial testing, disclosed in a preferred embodiment of this application.
[0024] Figure 4 A microbial separation device for pharmaceutical microbial testing, as disclosed in a preferred embodiment of this application. Figure 3 Enlarged structural diagram at point A in the middle;
[0025] The following are the labels in the diagram: 1. Outer shell; 11. Support column; 12. Drain valve pipe; 13. Mounting base; 2. End cap; 3. Storage tank; 31. Sealing cap; 32. Suction pipe; 33. Culture medium pump; 34. Infusion pipe; 35. Ring distribution pipe; 36. Outlet pipe; 4. Controller; 5. Centrifuge motor; 51. Flower-shaped frame; 6. Turntable; 61. Connecting sleeve; 611. Positioning bolt; 62. Conical container; 7. Ring heating frame; 8. Culture vessel; 81. Multilayer filter adsorption assembly. Detailed Implementation
[0026] The present application will be further described in detail below with reference to the accompanying drawings.
[0027] Reference Figures 1 to 4This application provides a microbial separation device for pharmaceutical microbial testing, comprising: a shell 1, an end cap 2 detachably and sealed at the upper end of the shell 1, a storage tank 3 and a controller 4 detachably installed outside the shell 1, a flower-shaped frame 51 fixedly installed at the upper inner end of the shell 1 by bolts, a centrifugal motor 5 fixedly installed on the flower-shaped frame 51, a turntable 6 drivenly connected to the output shaft of the centrifugal motor 5, a plurality of conical containers 62 and culture vessels 8 installed around the circumference of the turntable 6, the conical containers 62 and the culture vessels 8 being arranged in a one-to-one correspondence, and the culture vessels 8 being located at the bottom of the conical containers 62, a multi-layer filter adsorption assembly 81 detachably installed at the bottom of the culture vessels 8, a constant temperature heating device provided in the controller 4, and an annular heating frame 7 connected inside the shell 1, and a culture medium dispensing and conveying mechanism connected inside the storage tank 3 to the shell 1 for conveying culture medium solution into each conical container 62;
[0028] The multi-layer filtration and adsorption assembly 81 consists of a filter membrane layer and an adsorption layer arranged sequentially from top to bottom. The adsorption layer includes an activated carbon adsorption layer, a silica gel adsorption layer, and an antibacterial adsorption layer. In use, the microbial sample to be tested is pre-added into multiple conical containers 62 located on the turntable 6. Each conical container 62 has a corresponding incubator 8 installed at its bottom. The end cap 2 at the top of the outer casing 1 is then sealed and closed. The centrifugal motor 5 is electrically started via the controller 4. The output shaft of the centrifugal motor 5 drives the turntable 6 and the multiple conical containers 62 to rotate at high speed. Due to centrifugal force, microorganisms in the sample are thrown from the bottom of the conical containers 62 onto the filter membrane layer, achieving preliminary separation of microorganisms from other impurities. Furthermore, based on the different pore sizes of the filter membrane layer, different filter membrane layers retain a wider range of microorganisms. Different biological species are isolated to achieve different microbial purposes. By setting up multiple culture medium distribution and delivery mechanisms controlled by controller 4, the culture medium solution in storage tank 3 is injected into multiple conical containers 62 in appropriate amounts. The culture medium solution provides a growth environment for microorganisms through the filter membrane layer. During the culture process, the filter membrane layer prevents microorganisms from passing through, while various nutrients in the culture medium can be absorbed by microorganisms through the filter membrane layer. The activated carbon adsorption layer, silica gel adsorption layer and antibacterial adsorption layer play an adsorption role to remove impurities and harmful substances in the sample. The constant temperature heating device built into controller 4 can use the ring heating rack 7 to maintain the constant temperature environment inside the outer shell 1 under the required conditions, ensuring the purity of the microbial growth environment, facilitating subsequent observation and detection, avoiding interference from impurities, and thus meeting the needs of microbial growth.
[0029] Reference Figure 1 and Figure 2 The bottom of the outer shell 1 is fixedly provided with four pillars 11, and the bottom of the outer shell 1 is fixedly connected to a drain valve pipe 12. The outer shell 1 is made of transparent polycarbonate material, which gives the outer shell 1 a certain strength and corrosion resistance. By setting a transparent structure, it is easy to observe the internal situation.
[0030] Reference Figure 1 and Figure 2 The outer shell 1 is fixedly provided with two mounting bases 13. The storage tank 3 and the controller 4 are respectively fixedly mounted on one mounting base 13 by bolts. The outer shell 1 is limited by the mounting bases 13 to install the storage tank 3 and the controller 4. The storage tank 3 is sealed and stored with culture medium solution, which is used to provide culture medium solution to the filter membrane layer. The controller 4 can be used to regulate the centrifuge motor 5 and provide centrifugation speed and time parameters for different microorganisms.
[0031] Reference Figure 2 and Figure 3 The storage tank 3 is detachably and sealed with a cap 31. The culture medium distribution and delivery mechanism includes a culture medium pump 33, which is fixedly installed on the flower-shaped frame 51 by bolts. A suction pipe 32 is detachably connected between the culture medium pump 33 and the storage tank 3. A delivery pipe 34 is fixedly connected to the culture medium pump 33. An annular distribution pipe 35 is fixedly connected to the bottom of the delivery pipe 34. Multiple outlet pipes 36 are fixedly fixed along the circumferential thread of the annular distribution pipe 35 and are set one-to-one with the conical container 62. When it is necessary to provide culture medium solution to the filter membrane layer, the centrifugal motor 5 is paused and the conical container 62 is stopped at the bottom of the corresponding outlet pipe 36. The culture medium pump 33 is started by the controller 4, which can draw culture medium solution from the storage tank 3 and deliver it to the corresponding conical container 62 through the multiple outlet pipes 36 connected by the annular distribution pipe 35. The culture medium solution passes through the conical container 62 and permeates into the filter membrane layer, providing a good growth environment for microbial growth.
[0032] Reference Figure 3 and Figure 4 A connecting sleeve 61 is fixedly provided at the center of the turntable 6. The output shaft of the centrifugal motor 5 is movably inserted into the connecting sleeve 61 and is threaded with two positioning bolts 611. The output shaft of the centrifugal motor 5 and the connecting sleeve 61 are limited and fixedly connected by the two positioning bolts 611, so that the output shaft of the centrifugal motor 5 can drive the turntable 6 to rotate centrifugally inside the outer shell 1.
[0033] Reference Figure 3 and Figure 4The conical container 62 has multiple micropores evenly distributed at its conical bottom, with the diameter of the micropores ranging from 0.1 to 1 micrometer. Through these micropores, microorganisms and culture medium can pass through and be ejected onto the filter membrane layer. The filter membrane layer is made of cellulose ester microporous membrane, which has good biocompatibility and chemical stability. By selecting the pore size according to the type of microorganism, microorganisms can be intercepted while allowing nutrients in the culture medium to permeate, providing growth conditions for microorganisms. The multi-layer adsorption layer consists of three layers: the upper layer is an activated carbon adsorption layer, used to adsorb pigments and impurities in the sample; the middle layer is a silica gel adsorption layer, which further adsorbs moisture and other small molecules; and the lower layer is an antibacterial adsorption layer containing silver ion antibacterial substances, which can inhibit the growth of miscellaneous bacteria and prevent external bacteria from contaminating the sample during the separation process.
[0034] Working Principle: In use, the microbial sample to be tested is pre-added to multiple conical containers 62 located on a rotating disc 6 using a micropipette. Then, the end cap 2 at the top of the outer shell 1 is closed. A culture vessel 8 is installed at the bottom of each conical container 62 on the rotating disc 6. The centrifuge motor 5 is electrically activated via the controller 4, causing the rotating disc 6 to rotate at high speed along with the multiple conical containers 62 and culture vessels 8. Due to centrifugal force, microorganisms in the sample are ejected through the micropores at the bottom of the conical containers 62 onto the filter membrane, achieving preliminary separation of microorganisms from other impurities. Furthermore, based on the different pore sizes of the filter membrane, different types of microorganisms are retained in different filter membranes, achieving the purpose of separating different microorganisms. By setting an appropriate centrifugation speed and time, after centrifugation, the conical containers 62 can be observed through the transparent outer shell 1. At the bottom of the corresponding outlet pipe 36, the culture medium pump 33, controlled by the controller 4, can draw culture medium solution from the storage tank 3. The solution is then transported to the corresponding conical container 62 via multiple outlet pipes 36 connected by the annular distribution pipe 35. The culture medium solution passes through the conical container 62 and permeates into the filter membrane layer, providing a good growth environment for microorganisms. During the culture process, the filter membrane layer prevents microorganisms from passing through, while various nutrients in the culture medium can be absorbed by the microorganisms through the filter membrane layer. The activated carbon adsorption layer, silica gel adsorption layer, and antibacterial adsorption layer play an adsorption role, removing impurities and harmful substances from the sample. Furthermore, the constant temperature heating device built into the controller 4 can maintain the interior of the outer shell 1 at the required constant temperature environment using the annular heating rack 7, ensuring the purity of the microbial growth environment, facilitating subsequent observation and detection, and avoiding interference from impurities, thereby meeting the needs of microbial growth.
Claims
1. A microbial separation device for microbial testing of pharmaceuticals, characterized in that: Includes: an outer shell (1), with an end cap (2) detachably and sealed at the upper end of the outer shell (1); a storage tank (3) and a controller (4) detachably installed on the outside of the outer shell (1); a flower-shaped frame (51) fixedly installed on the upper inner part of the outer shell (1) by bolts; a centrifugal motor (5) fixedly installed on the flower-shaped frame (51); a turntable (6) is driven by the output shaft of the centrifugal motor (5); and multiple conical containers (62) and incubators (8) are installed on the turntable (6) along its circumference. The conical container (62) and the culture vessel (8) are arranged in a one-to-one correspondence, and the culture vessel (8) is located at the bottom of the conical container (62). A multi-layer filter adsorption assembly (81) is detachably installed at the bottom of the culture vessel (8). The controller (4) is equipped with a constant temperature heating device and is connected to a ring heating frame (7) inside the outer shell (1). The storage tank (3) is connected to a culture medium distribution conveying mechanism inside the outer shell (1) for conveying culture medium solution into each conical container (62). The multi-layer filtration and adsorption assembly (81) is provided with a filter membrane layer and an adsorption layer from top to bottom. The adsorption layer includes an activated carbon adsorption layer, a silica gel adsorption layer and an antibacterial adsorption layer.
2. The microbial separation device for pharmaceutical microbial testing according to claim 1, characterized in that: The bottom of the outer shell (1) is fixedly provided with four support pillars (11), and the bottom of the outer shell (1) is fixedly connected to a drain valve pipe (12).
3. The microbial separation device for microbial testing of pharmaceuticals according to claim 1, characterized in that: The outer shell (1) is fixedly provided with two mounting bases (13), and the storage tank (3) and the controller (4) are respectively fixedly mounted on one mounting base (13) by bolts.
4. A microbial separation device for microbial testing of pharmaceuticals according to claim 1, characterized in that: The upper end of the storage tank (3) is detachably sealed with a cap (31). The culture medium distribution and delivery mechanism includes a culture medium pump (33). The culture medium pump (33) is fixedly installed on the flower frame (51) by bolts. The culture medium pump (33) and the storage tank (3) are detachably connected by a suction pipe (32). The culture medium pump (33) is fixedly connected to a delivery pipe (34). The bottom end of the delivery pipe (34) is fixedly connected to an annular distribution pipe (35). The annular distribution pipe (35) has multiple outlet pipes (36) fixed along the circumferential thread and is set one-to-one with the conical container (62).
5. A microbial separation device for microbial testing of pharmaceuticals according to claim 1, characterized in that: A connecting sleeve (61) is fixedly provided at the center of the turntable (6). The output shaft of the centrifugal motor (5) is movably inserted into the connecting sleeve (61), and two positioning bolts (611) are threadedly fixed therein.
6. A microbial separation device for microbial testing of pharmaceuticals according to claim 1, characterized in that: The conical container (62) has a plurality of micropores uniformly arranged at its conical bottom, and the diameter of the micropores is set between 0.1 and 1 micrometer.