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29 results about "Cambium" patented technology

A cambium (plural cambia or cambiums), in plants, is a tissue layer that provides partially undifferentiated cells for plant growth. It is found in the area between xylem and phloem. It forms parallel rows of cells, which result in secondary tissues.

Method for improving grafting survival rate of malania oleifera

PendingCN121241803AGraftingHorticulture methodsRootstockCambium
The invention relates to the technical field of plant grafting, in particular to a method for improving the grafting survival rate of malania oleifera, which comprises the following steps: S1, scion preparation: treating branches of excellent perennial wild malania oleifera plants in the field, conveying the branches to an inoculation field, and disinfecting to obtain disinfected branches; s2, stock preparation; s3, grafting: preparing a scion by using the disinfected branch, treating a stock, tightening the stock and a grafting film for tongue grafting of the scion, and smearing a healing agent on a cut of the cut stock and the upper end of the grafting film; s4, management after grafting. Compared with the prior art, the method has the advantages that superior plant branches and artificially cultivated stocks are subjected to tongue grafting, and compared with bud grafting, the yield time of grafted seedlings is shortened; and then the branch of the malania oleifera superior plant is treated immediately when the scion is collected, so that the problems that the activity of the branch is reduced in the transportation process and the formation of the callus of the stock and scion cambium is slow are solved, the grafting survival rate is increased, and the grafted seedling obtaining time is shortened.
Owner:GUANGXI FORESTRY RES INST

Ilex rotunda grafting method

The invention discloses a Ilex rotunda grafting method which comprises the following steps: from late February to mid-March, preparing a 3-5-year-old Ilex rotunda rootstock for cultivation, and irrigating the Ilex rotunda rootstock; preparing a scion with double inclined planes; cutting a cut with the depth of 3cm in the rootstock, inserting the scion and aligning to the cambium; and carrying out double-layer binding by using a viscous PVC grafting film and a grafting soft film. The final-period management comprises the steps of sunshelter building, irrigation, bud picking, unbinding, pinching, fertilization and weeding. The problem that Ilex rotunda is difficult to breed is solved, the grafting survival rate is high, and the seedling raising period is short.
Owner:VEGETABLE & FLOWER INST JIANGXI ACADEMY OF AGRI SCI

Method for cultivating polyploidy by inducing doubling of girdling calluses of branches and trunks of camellia oleifera

The invention relates to a method for inducing doubling cultivation of polyploidy by girdling calluses of camellia oleifera branches, the influence on tree vigor is reduced through girdling, the efficiency of regeneration buds is improved through thorough removal of cambium, the bottleneck in the prior art is overcome, chimera-free polyploidy material induction can be realized without in-vitro tissue culture, and the method is suitable for large-scale popularization and application. The method is simple and convenient to operate and low in cost, can effectively avoid the problems of rooting difficulty of tissue culture materials, transplanting loss of tissue culture seedlings and the like, provides a new technical approach for woody plant polyploid breeding, and has important popularization and application values.
Owner:CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY

A method and system for generating an intelligent trajectory for adhesive liquid collection operation and a medium

PendingCN122289531ALiving treeComputer graphics (images)
This invention belongs to the field of rubber tapping technology and discloses a method, system, and medium for generating intelligent latex tapping operation trajectories. The method includes: acquiring X-ray images of living trees in a preset area; performing grayscale conversion and filtering on the X-ray images to obtain processed images; constructing a three-dimensional envelope model of the living tree based on the processed image, the three-dimensional envelope model of the living tree including a bark envelope model and a core-edge envelope model; generating a tapping trajectory based on the bark envelope model and the core-edge envelope model, the tapping trajectory including the bark cutting length, cutting direction, and cutting depth. This invention can accurately identify the boundaries between the latex layer and cambium layer of rubber trees, and then plan its own tapping trajectory through the three-dimensional envelope model of each rubber tree, realizing efficient and accurate intelligent rubber tapping operations.
Owner:SICHUAN SANZEQI ROBOT CO LTD

A method for isolating and culturing a formation layer stem cell of burdock

This invention discloses a method for inducing and culturing burdock stem cells and stem cells, belonging to the field of plant biotechnology. Using burdock roots as explants, the cambium is determined by staining, and the cambium is obtained using enzymatic digestion. The cambium is then inoculated into a specific ratio of induction solid medium and cultured in the dark to efficiently induce primary stem cells. These primary stem cells are then transferred to a proliferation solid medium for subculture and selection to obtain and maintain a vigorous, soft stem cell line. The solid stem cell line is further transferred to a liquid medium for shaking culture to establish a suspension stem cell line. This invention provides high induction efficiency, stable stem cell characteristics, and good reproducibility, offering a high-quality starting material and technical platform for in vitro preservation of burdock germplasm, large-scale production of secondary metabolites, and genetic transformation.
Owner:XUZHOU NORMAL UNIVERSITY +1

Method for promoting ginsenoside accumulation in ginseng cambium stem cells

The invention belongs to the technical field of biotechnology and plant tissue culture, and particularly relates to a method for promoting ginsenoside accumulation in ginseng cambium stem cells. According to the method for promoting ginsenoside accumulation in the ginseng cambium stem cells, efficient induction and accumulation of ginsenoside in the ginseng cambium stem cells are promoted by optimizing culture conditions and induction strategies. The ginseng cambium stem cells obtained by the method have high ginsenoside content, and especially saponin components such as Ro, Re, Rg2, Rh1 and the like are remarkably improved.
Owner:CHANGZHOU LONGZANG BIOTECHNOLOGY CO LTD

Big tree bionic supporting and protecting device

The invention discloses a big tree bionic supporting and protecting device which comprises two symmetrically-arranged supporting bases, supporting blocks are arranged at the tops of the ends, close to each other, of the supporting bases, and arc-shaped grooves are formed in the ends, close to each other, of the supporting blocks; the device further comprises a bionic supporting device and a supporting bottom rod installed on the supporting block through a bolt, a supporting rod is movably connected to the supporting bottom rod, the outer side wall of the supporting rod is connected with a movable supporting rod through a supporting assembly, and the bottom end of the movable supporting rod is movably connected to the side wall of the supporting bottom rod. The bionic structure has the advantages that the D printing lignin composite material in the arc-shaped groove adopts a gradient density design, and the surface degradation rate is matched with the tree cambium growth rate; a pre-etched tree-shaped degradation path guides the new bark to heal along a specific form, and annular tightening marks of a traditional stent are avoided; the friction coefficient of the contact surface is increased through honeycomb-shaped textures, and meanwhile micro-airflow channels are formed to reduce the humidity of the barks.
Owner:HUAYI ECOLOGICAL LANDSCAPE ARCHITECTURE

A method for seedling raising of nothosuchowia zanthoxyloides by grafting

The application discloses a kind of nothocinnamomum officinale grafting seedling raising methods, belong to the technical field of pepper grafting seedling, the method includes the following steps: (1) top pot pepper stock seedling cultivation: select 2-year-old disease-resistant, drought-resistant, well-growing top pot pepper big green pepper variety seedling as stock;(2) nothocinnamomum officinale scion collection: select current year nothocinnamomum officinale branch as scion, top of pruned scion, compound leaf, get grafting scion;(3) grafting: on the same stock, complete first branch grafting in late December-2 late, complete second bud grafting in late May-early July;(5) management after grafting: grafting survival carries out fertilization and weeding, solve the problem of low grafting survival rate caused by that cambium does not completely adhere when grafting.
Owner:GUIZHOU WALNUT RES INST

Water-soluble plant wound healing agent and method of preparation

The present application provides a water-soluble plant wound healing agent and a preparation method. The preparation raw materials of the healing agent include water, water-soluble lanolin, mussel mucopolysaccharide, sodium naphthaleneacetate, willow fresh branch extract and copper sulfate. The healing agent can form a thin film with strong water retention and air permeability when sprayed on the wound of the tree body, preventing water evaporation in the wound xylem, inducing the cambium cells to split faster and promoting the rapid growth of the cambium. Meanwhile, the healing agent can prevent external bacteria from entering the wound of the tree body, achieving the effects of bacteriostasis and sterilization, and avoiding secondary damage caused by pathogenic bacteria infection. After the wound is recovered, the water-soluble lanolin disappears due to volatilization and rainwater flushing, avoiding the burn caused by the invasion of oily substances into the bark. The healing agent has good liquid flowability, film-forming property and water retention, and the raw materials are simple and easy to obtain. The preparation process is simple, and the healing agent can be used to heal the wound under complex conditions such as high position, large area and large quantity by means of a spray pot, a small sprayer and the like, greatly improving the work efficiency.
Owner:SHANDONG INST OF POMOLOGY

Large-trunk-diameter fruit tree bark grafting method using polystyrene foam for sealing and fixing

PendingCN121312419AGraftingBiotechnologyCambium
The invention relates to a large-trunk-diameter fruit tree bark grafting method for sealing and fixing by using polystyrene foam, which comprises the following steps: wrapping a thin plastic film on the cross section of a trunk of a stock to effectively isolate external air and moisture, prevent invasion of germs and reduce water loss; the plastic film can effectively prevent polystyrene foam in later construction from invading a grafting interface, and the problem that interface healing is blocked due to pollution is solved. After the scion is inserted through the marked grafting point, the grafting position of the scion and the stock is fixed through polystyrene foam, a stable protection layer is formed after the polystyrene foam is cured, the joint healing quality is effectively improved, and meanwhile the scion fixing strength is enhanced. The protective layer has good heat preservation and moisture preservation performance, and cambium cell division and healing tissue generation are promoted. After the polystyrene foam is completely cured, the outside is wound and wrapped by a shading film, so that ultraviolet degradation is avoided, and water evaporation is reduced. The whole grafting structure is stable, resistant to wind and rain erosion and suitable for efficient standardized operation of large-scale orchards, and the technical threshold and the labor cost are remarkably reduced.
Owner:BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES

Method and device for improving yield and quality of chrysanthemum morifolium by grafting artemisia annua

ActiveCN117426217BFlowers cultivationGraftingRootstockCambium
The application discloses a method and device for grafting artemisia annua to improve yield and quality of chrysanthemum morifolium, and belongs to the technical field of grafting, comprising the following steps: step 1: selecting materials, selecting healthy chrysanthemum morifolium scion as stock, and selecting artemisia annua as rootstock; the rootstock requires thick and strong roots, is free of diseases and pests, and is located on a flat ground with sufficient sunlight and no other obstructions, so as to facilitate subsequent growth and development; step 2: cutting the stock and disinfecting, using a grafting device to cut a grafting incision at the end of the chrysanthemum morifolium scion; the incision requires that the cortex is cut off, and the cambium layer is visible; when the grafting device cannot form at one time, a blade is adjusted, and the length of the cutting is 2-3 cm; the method and device for grafting artemisia annua to improve yield and quality of chrysanthemum morifolium are provided, and are used for solving the problems of low yield and quality of chrysanthemum morifolium products, and the problems of difficulty in cutting the incision of the rootstock during grafting, difficulty in healing the incision after grafting, poor growth quality and low quality.
Owner:HUNAN INST OF GARDENING

A method for growing actinidia arguta

ActiveCN116548194Bwell-developed rootsImprove cultivation adaptabilityActinidia valvataRootstock
This invention discloses a method for cultivating hardy kiwifruit, comprising the following steps: S1 Cultivating male 'Jinmi Rootstock No. 1' seedlings; S2 Grafting, starting in mid-to-late February, high-position grafting is performed on the 'Jinmi Rootstock No. 1'; First, a grafting method with a grafting diameter of 0.8 cm is used: the scion is 5-7 cm long, each segment retains 2-3 buds, and the lower end is cut into an oblique surface; the rootstock is cut at a diameter of 0.8 cm, then longitudinally cut, with the cut size similar to that of the scion; then the cambium layers of the scion and rootstock are aligned and tied tightly with a thin film strip. Beneficial effects: 'Jinmi Rootstock No. 1' can increase the average yield of hardy kiwifruit cultivars by 25.7%, showing a significant yield increase; 'Jinmi Rootstock No. 1' has a very well-developed root system, strong vitality, and can be directly propagated by cuttings, making seedling cultivation easy; it improves the adaptability of hardy kiwifruit cultivation and reduces the rate of tree death caused by waterlogging.
Owner:INST OF FRUIT & TEA HUBEI ACAD OF AGRI SCI

Poplar PdPXC1 gene and application thereof in improving poplar growth and wood quality

The invention discloses application of a poplar PdPXC1 gene in regulating poplar growth, xylem development and cell wall component synthesis. A nucleotide sequence of a coding region of the PdPXC1 gene is shown as SEQ ID NO: 1, and an amino acid sequence coded by the PdPXC1 gene is shown as SEQ ID NO: 3. A PdPXC1 over-expressed or gene-edited transgenic poplar plant can be obtained by constructing a recombinant expression vector containing the gene or a CRISPR / Cas9 gene editing vector and transforming the poplar. Compared with a wild type, the plant height, the ground diameter, the cambium cell number, the xylem width, the cellulose content, the lignin content and the fiber cell wall thickness of the overexpressed plant are all remarkably increased; and the gene editing plant shows an opposite phenotype. The invention provides an important gene target and a breeding material for directionally improving the growth speed of the poplar and the wood quality through a genetic engineering means, and has a wide application prospect in forestry production.
Owner:QINGDAO AGRI UNIV

High-hydraulic insoluble fiber raw material, its preparation method and application

The application provides a high water-retention insoluble fiber and a preparation method and application thereof. The high water-retention insoluble fiber used in the application is derived from wood and wood chip processing by-products, and is obtained through a technical process of removing bark and cambium, cutting, crushing, high-temperature vaporization, pickling and drying, has a water-retention of 634-789%, an insoluble fiber component content of 84.32-88.24%, and a soluble fiber component content of 1.23-1.62%, and can be added into the feed of pregnant sows as a functional fiber raw material, so as to improve the constipation and empty chewing ratio of the pregnant sows, increase the number of live piglets and weaning litter weight of the sows and the mating rate in the next reproduction cycle, reduce the empty chewing ratio in the late pregnancy (100-102d) of the sows and the elimination rate in the next reproduction cycle, and has a wide application prospect in the daily feed of the pregnant sows.
Owner:CHINA AGRI UNIV

Method for improving salt tolerance of woody plants through interspecific grafting

The invention discloses a method for improving the salt tolerance of a woody plant through interspecific grafting, in particular to a method for improving the salt tolerance of a scion through grafting by taking a salt-tolerant variety as a stock, and relates to the field of grafting planting. The method comprises the following steps: (1) cutting an annual branch of a salt-tolerant woody plant as a stock, and cutting a phloem at the upper end and the middle part of a cambium from top to bottom; (2) cutting an annual branch of the woody plant and reserving 2-3 buds as scions; (3) aligning and abutting cambiums on the two sides of the long cutting surface of the scion and cambiums on the two sides of the cut of the stock, and binding with a plastic film strip; after the grafting succeeds, one vigorous leaf bud is reserved in the scion. According to the method, the populus nigra scion is grafted to the salt-tolerant American black poplar stock, so that the salt tolerance of the populus nigra scion is improved, the obtained grafted seedling has better growth advantage and salt tolerance, and the method has important application value in increasing the forest yield and cultivating new salt-tolerant varieties.
Owner:LUDONG UNIVERSITY +1

Application of knocking out poplar DOF9 gene in improving wood yield and method thereof

ActiveCN120138042BBiotechnologyCambium
The application discloses knockout Populus DOF9 gene in improving wood yield and a method thereof, a DOF9 knockout plant carrier is introduced into a Populus deltoids cv. 'Nanlin 895' to obtain a DOF9 knockout transgenic plant; the stem xylem proportion of the knockout plant is significantly higher than that of a wild type (WT), and the xylem layer number is also significantly increased; the result shows that the stem cambium activity and xylem differentiation of the Populus are promoted after the DOF9 is deleted, and the development and utilization of the Populus have important significance.
Owner:SOUTHWEST UNIV

Method for high branch layering propagation of malmea fruit

ActiveCN117652512BBiocideLayeringCambiumSurface-active agents
The application belongs to the technical field of propagation of Artocarpus heterophyllus, and particularly relates to a high-branch layering propagation method of Artocarpus heterophyllus. The high-branch layering propagation method of Artocarpus heterophyllus comprises the following steps: S1. selecting a single branch with a healthy growth state and performing ring peeling; S2. after the cambium is scraped off, a plastic film is wrapped around the upper end of the ring peeling; S3. after the plastic bag is longitudinally cut, the plastic bag is wrapped around the ring peeling position and fixed on the branch; and S4. after the upper end of the ring peeling is fully rooted, the branch is pruned and transplanted. In step S3, 0.75-1.5% of a fungicide is added to the layering propagation matrix, the fungicide is prepared by using a ternary compound of benzene ether carbendazim, oxime ether fungicide and zinc pyrithione as active ingredients, and is supplemented with a surfactant and a solvent. The propagation method can effectively prevent the influence of root rot pathogenic bacteria on the rooting of the branch, is beneficial to the rooting of the high-branch layering, and can further guarantee the survival rate and the propagation efficiency of the high-branch layering, and is beneficial to the smooth development of the seedling raising work of Artocarpus heterophyllus.
Owner:GUANGXI SUBTROPICAL CROPS RESEARCH INSTITUTE(GUANGXI SUBTROPICAL AGRICULTURAL PRODUCTS PROCESSING RESEARCH INSTITUTE)

Application of knocking out poplar aco gene in improving wood yield and method thereof

ActiveCN120158480BIncrease productionincrease plant heightBiotechnologyCambium
The application discloses knockout poplar ACO gene in the application of improving wood yield and a method thereof, an ACO knockout plant carrier is introduced into NL895 to obtain an ACO knockout transgenic plant; the stem xylem proportion in the knockout plant is significantly higher than that of WT, and the xylem layer number is also significantly increased; the result shows that the poplar stem cambium activity and xylem differentiation are promoted after the ACO is deleted, and the poplar development and utilization have important significance.
Owner:SOUTHWEST UNIV

Method for grafting pinus massoniana with pinus yunnanensis

ActiveCN120858760BBiocidePlant growth regulatorsShootCambium
The application provides a method for grafting Pinus tabulaeformis with Pinus yunnanensis var. biondii, and belongs to the technical field of grafting of needle-leaved trees, and solves the technical problems of slow grafting, high pathogenic infection rate and low survival rate of less than 40% caused by environmental fluctuations. The technical solution points comprise the following steps: cutting a vertical incision of 1.8-2.2 cm deep to the xylem at a position of 2.5-3.5 cm from the surface of the cultivation substrate exposed at the root stem of the stock; immersing the base of the scion into a sodium citrate buffer solution containing polyvinylpyrrolidone, methyl salicylate and calcium chloride after the base is cut into a symmetrical double-wedge shape; coating adsorption glue on both sides of the vertical incision; inserting the scion to align the cambium and fixing the scion with a grafting tape; applying an aqueous ethanol solution containing salicylic acid, trehalose and polysorbate 80 to the grafting interface every 2-3 days; maintaining the day temperature at 23-25 DEG C, the night temperature at 16-18 DEG C and the humidity at 90-95%; girdling the stock when the new shoots grow to 2-3 cm in length, and cutting off the main stem after 7-10 days. The application is used for large-scale propagation of Pinus yunnanensis var. biondii grafted seedlings.
Owner:INNER MONGOLIA PINECONE ECOLOGICAL TECHNOLOGY CO LTD

Separation and differentiation culture method of salvia miltiorrhiza root cambium stem cells

The invention belongs to the technical field of plant stem cell culture, and particularly relates to an isolation and differentiation culture method of salvia miltiorrhiza root cambium stem cells, which comprises the following steps: cutting the root of a wild salvia miltiorrhiza plant into sections, washing and sterilizing, cutting into small sections, sterilizing for the second time, and washing to obtain a sterile explant. Or carrying out pretreatment, surface sterilization and culture on salvia miltiorrhiza seeds to obtain sterile seedlings, carrying out expanding propagation and rooting and seedling strengthening culture to obtain sterile plants, and cutting the roots of the sterile plants into sections to obtain the sterile explants. And soaking the sterile explant in a preserving fluid, and then carrying out induction culture, subculture and suspension culture to obtain the salvia miltiorrhiza root cambium stem cells. Wherein the preserving fluid, the induction culture medium, the subculture medium and the suspension culture medium all contain plant hormones. And the induction culture medium also contains REF1. According to the separation and differentiation culture method of the salvia miltiorrhiza root cambium stem cells, cell browning can be effectively prevented, and the cultured tissue cells are stable in gene expression and growth.
Owner:GUANGDONG MEDICAL UNIV +1

A method for simulating broadleaf tree wood formation and radial growth using climate data

PendingCN122655360ADividing cellCambium
The application discloses a method for simulating wood formation and radial growth of broad-leaved trees by using climate data and belongs to the technical field of tree growth prediction. In order to solve the problem of accurate simulation of wood formation and radial growth of broad-leaved trees, the application comprises the following steps: collecting input data including latitude data of a target region and long-term daily meteorological data of the target region; establishing cambium activation conditions of the broad-leaved trees; calculating relative growth rates of cell expansion, cell wall deposition and cambium cells, and calculating expansion rates of the cambium cells; establishing a cambium cell division model and a vessel cell differentiation cycle model; establishing a cell expansion model and a cell wall thickening model, simulating the growth process of the cells in the xylem until the cell wall lignification process is completed, and then the cells enter a mature cell stage; and based on the mature cell stage, calculating annual ring width and anatomical characteristics, and completing the simulation process of simulating the wood formation and radial growth of the broad-leaved trees by using the climate data.
Owner:NORTHEAST FORESTRY UNIV

Humidity control long block bud grafting method and special biological active dressing for improving survival rate of ginkgo grafting

The application discloses a humidity control and long block bud grafting method for improving the survival rate of ginkgo grafting and special biological active dressing. The method comprises the following steps: pretreating the scion with 50-100 mg / L mannitol solution for 30-60 minutes; cutting a long block bud piece with a thin layer of xylem, the long block bud piece being 3.0-4.0 cm long and 1.5-2.5 cm wide, and ensuring that the cambium is precisely connected; smearing the grafting interface with special dressing containing 0.01%-0.05% indole butyric acid and 0.5%-1.0% carbendazim; and wrapping the grafting interface with a degradable grafting film, and keeping the temperature at 20-28 DEG C and the humidity at greater than or equal to 80% until callus formation. The dressing is composed of 0.01%-0.05% indole butyric acid, 0.5%-1.0% carbendazim and the rest of the base material in percentage by mass. The application solves the problems of water loss, slow healing and susceptibility to infection in ginkgo grafting by combining endogenous water retention, exogenous healing promotion and standard operation, and the survival rate is stably improved to more than 95%, and the optimal survival rate is 98%. The method is simple to operate and suitable for large-scale promotion.
Owner:QINGDAO AGRI UNIV +1

Ginseng adventitious root cell strain CCUM-Re-7 as well as extract, preparation method and application thereof

The invention discloses a ginseng adventitious root cell strain CCUM-Re-7 as well as an extract, a preparation method and application thereof. A construction method of the cell strain CCUM-Re-7 comprises the following steps: performing double disinfection and sterilization on ginseng, performing culture amplification after slicing, and separating into layered stem cells; inducing the separated cambium stem cells to generate ginseng adventitious roots; culturing the induced ginseng adventitious roots, and screening a cell strain CCUM-Re-7 from the induced adventitious roots; and carrying out large-scale culture on the cell strain to extract and prepare the ginseng adventitious root extract rich in saponin, and taking the ginseng adventitious root extract as an effective component to prepare the anti-aging and whitening composition. The invention belongs to the technical field of biology, and particularly provides a method for extracting an extract rich in ginsenoside Re from ginseng adventitious roots induced by ginseng cambium stem cells and application of the extract in anti-aging and skin whitening.
Owner:CHANGCHUN UNIV OF CHINESE MEDICINE

Method for repairing wounds of tree stems

The invention relates to the technical field of garden maintenance, and discloses a method for repairing wounds of tree stems. The repairing method comprises the following steps: S1, a root system induction stage: firstly carrying out cleaning, disinfection and preservative treatment on a wound surface, then obliquely cutting the upper edge of the wound surface to expose a cambium, smearing a root system inducer on an incision, then covering a peat soil matrix, and carrying out sealing, moisturizing and shading maintenance; s2, a root system traction stage, wherein adventitious roots are guided to the ground through a traction pipe, and water and fertilizer management is conducted; s3, a root system lignification promoting stage: promoting root system lignification through illumination control, exogenous chemical regulation and periodic water control. According to the repairing method, tree stem wound bark is induced to rapidly generate adventitious roots, a root system is introduced into the ground through a traction pipe and water and fertilizer induction, the nutrient transportation function of the bark is recovered through illumination control, exogenous chemical regulation and control, periodic water control and other measures, the tree is made to recover the normal growth vigor, operation is easy, environment adaptability is high, universality is high, and the method is suitable for large-scale popularization and application. The survival rate is high.
Owner:PUBANG LANDSCAPE ARCHITECTURE CO LTD +1

Koelreuteria paniculata grafting method

The invention belongs to the technical field of horticulture, and particularly relates to a koelreuteria paniculata grafting method which comprises the following steps: grafting by using a cambium docking method by taking a koelreuteria paniculata seedling which is more than two years old as a stock and a koelreuteria paniculata stock crown branch as a scion; wherein before grafting, 2mL-3mL of a grafting protective agent No.1 is sprayed at the grafting part of the rootstock; after grafting, 1.5 mL-2. 5mL of grafting healing agent No.2 is sprayed on the grafting part of the scion; the first grafting protective agent is prepared from Cinnamomum camphora, cordate houttuynia, water and ethanol, the second grafting healing agent is prepared from a chitosan solution, an auxin solution, a kinetin solution, Cinnamomum camphora essential oil and Tween 80, and a mixed root system derived from a stock and a scion is formed, so that the defect of grafting incompatibility is fundamentally eliminated.
Owner:北京煜坤盛科技发展有限公司

Affinity agent for jujube tree twig grafting, preparation method and application thereof

The invention provides an affinity agent for jujube tree twig grafting and a preparation method and application thereof, and relates to the technical field of jujube tree cultivation. The affinity agent is prepared from the following components in mass concentration: 5 to 15g / L of saccharose, 50 to 100mg / L of naphthylacetic acid, 50 to 100mg / L of auxin and 5 to 15mg / L of gibberellin. According to the multi-hormone synergistic jujube tree twig grafting affinity agent provided by the invention, through scientific proportioning of nutrient substances and plant hormones, the viability of stock-scion cambium cells is activated, and rapid formation of calluses is promoted; meanwhile, a set of standardized twig grafting technology system based on the affinity agent is constructed, grafting operation and postoperative management processes are optimized, and efficient healing of rootstocks and scions and robust growth of nursery stocks are achieved. The technology is easy and convenient to operate, the twig grafting technology threshold is lowered, the adaptability of different jujube varieties and stock combinations is improved, and the requirements for large-scale seedling raising and variety renewal are met.
Owner:TARIM UNIV

A method for grafting propagation of sinocalyx xylochrous'red fortune'

PendingCN122498364ASolve the industry problem of inability to bud in early springIncreased activity of divisionRootstockCambium
This invention discloses a grafting propagation method for the summer wax plum 'Hongyun'. The method involves applying nitrogen fertilizer, boron fertilizer, and humic acid in the autumn of the previous year to build up energy reserves. In early March of the same year, a compound hormone of GA3+6-BA+NAA is sprayed three times. Combined with black plastic film wrapping to increase temperature and block light, black plastic film mulching to retain moisture, and ridge planting with frequent water management, the cambium layer of the wax plum rootstock and summer wax plum scion branches becomes active and ready for peeling in early spring. At this time, large-block budding is performed, resulting in high scion utilization. The buds sprout in early April, and the scions can be ready for transplanting in November of the same year. This method achieves a grafting survival rate of over 82%, which is more than 16 percentage points higher than traditional summer budding. The transplanting rate in the same year reaches 88%, the seedling cycle is shortened by one year, and the scion utilization rate is increased by 110%. This provides reliable technical support for the large-scale propagation of the rare new variety 'Hongyun'.
Owner:INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI

Clausena lansium grafting rootstock and scion preparation tool and clausena lansium grafting method

PendingCN121605867AGraftingRootstockCambium
The invention discloses a clausena lansium grafting rootstock and scion preparation tool and a clausena lansium grafting method. The preparation tool comprises a sleeve, a blade base and a cutting cutterhead, a baffle is fixed to the front end of the sleeve, and the rear end of the sleeve is sleeved with a holding part. The blade base is installed in the sleeve and can move in the axial direction of the sleeve. The base fixing device is used for fixing the blade base at a set position in the sleeve; the cutting cutterhead comprises a middle blade, a first side blade and a second side blade; one side of the cutting cutterhead is a stock preparation cutting area, and the other side of the cutting cutterhead is a scion preparation cutting area; one end of the cutting cutterhead is fixedly connected with the blade base; the baffle is provided with an open hole for the cutting cutterhead to penetrate through. The length of the cutting cutterhead extending out of the baffle is adjusted by adjusting the position of the blade base in the sleeve. When the preparation tool is used for grafting, the butt joint area and butt joint sufficiency of the cambium can be improved, so that the overall grafting survival rate is increased, and the problem of survival rate fluctuation between different grafting batches is solved.
Owner:BEIJING YALU ANCIENT TROPICAL BOTANICAL GARDEN CO LTD

A dwarfing and high-yield planting method for grafting old bombax malabarica onto heteroceiba

PendingCN122349878AShootCambium
This invention discloses a method for grafting old kapok trees onto *Ceiba speciosa* rootstock to achieve dwarfing and high yield, relating to the field of Bombacaceae plant cultivation technology. The method includes the following steps: selecting robust, dwarfing *Ceiba speciosa* seedlings with a diameter ≥1.5cm and a height of 60-80cm as rootstock; selecting plump bud segments with a diameter of 0.8-1.2cm from the middle of 1-2 year old branches of superior 10-20 year old kapok trees as scions; and grafting the rootstock 30-40cm above the ground 15-20 days before bud break from late February to early March. Graft at 0cm, align the cambium layers and tie; when the new shoots grow to 10-15cm, untie, remove the lateral buds of the rootstock and erect supports for wind protection; cultivate an open-center crown through trunk setting and heavy winter pruning, controlling the height of mature trees to 2.5-3.5m; apply fertilizer in stages and keep the soil moist; this invention achieves the goal of controllable dwarfing, high yield and quality, early fruiting and preservation of the original edible quality of old kapok by using the core technology combination of grafting local edible old kapok onto kapok rootstock and supporting cultivation management.
Owner:CHENGDU FUXIAOMAN CATERING MANAGEMENT CO LTD