277 results about "Cambium" patented technology

The invention discloses a stem cell tissue culture seedling raising technique. The technique comprises the steps of 1, taking a fresh plant, conducting sterilization on the surface and cutting the plant; 2, conducting tissue differentiation and reserving a cambium; 3, culturing the cambium on an isolation medium. According to the technique, existing methods are improved based on a traditional tissue culture technique, plant stem cells are taken as a target, induction, separation and explant culture are conducted, and a corresponding stem cell culture system is established. By the adoption of the plant stem cell culture technique, the plant culture technique is enriched, and a novel research direction and opportunity is provided for commercialized production of natural products and development of the plant biotechnology.

Compositions and methods are provided for treatment of cartilage defects in animals and humans. The compositions of the invention include synovial tissue, synovial cells and matrices containing synovial (or cambium) tissue or cells for use in filling a cartilage defect. The matrix and synovial tissue or cell preparations may also contain a proliferation agent, transforming factor or other active agents to promote healing. A controlled-release delivery system may be used to administer the transforming factor. The compositions of the invention also include a synovial covering membrane or devitalized fascial sheet for covering the cartilage defect. The methods of this invention are those in which a minimally invasive surgical intervention is performed to remove a small portion of synovial membrane from a joint. Portions of the synovial membrane, or cells expanded in vitro, are implanted alone or within a matrix, into the defect site, where they produce new cartilage tissue and repair the defect. Alternatively, partially transformed synovial-derived tissue may be formed in situ and implanted into the defect site.

The invention discloses a rejuvenation-type repairing method of holes or depressed defects of rare old trees, comprising the steps of manufacturing a primary joint surface of a stock on the cambium part of the laburnum at the bottom of the slit of the old tree; grafting a plurality of scions on the joint surface of the stock; after surviving, manufacturing a second joint surface of the stock attaching the scions on the cambium part of the laburnum on the upper part of the slit of the old tree; finally, manufacturing third joint surfaces between the slit and the scions, and the scions, so that the broken laburnums on the holes or depressed defects of the old tree trunk are communicated again by bridge function of the scions and the substances in the tree are transported vertically and thedepressed defects are supported well; thus the old tree is saved, rejuvenated and repaired, and the unique landscape symbolic significance and carried historical and cultural significance of the old tree are continued; thus the method has research value, ecological benefit, economic benefit and social benefit.

The invention relates to a technology for propagating an aleurites moluccana seedling by high-altitude layering. According to the technology, an operating method comprises the following steps of: selecting branches of which the sizes are similar and the growth conditions are excellent from the aleurites moluccana tree which has more than 4 age from March to October in every year; performing girdling on the stem bases of the selected branches, wherein the width is about 2 to 4 centimeters, and a cortex layer and a cambium are scraped completely; coating a growth regulator with appropriate concentration at the upper ends of incisions; making a wet matrix into a mud pie, covering wounds with the mud pie, wrapping the outside of the mud pie by using a plastic film, and binding by using a packing rope; and checking the humidity of the mud pie during layering rooting, and when the humidity is low, replenishing moisture by an injection method to keep the humidity of soil, wherein after the girdling is performed, roots are grown at the wounds a month later; and cutting the layering for transplant when a large quantity of root systems are formed two or three month later.

The invention aims to provide a grafting method with minimum cut area for improving grafting survival rate, in particular a method for grafting different plants on the same plant at high survival rate and with simpleness, feasibility and wide application range. The grafting method comprises the following steps: (1) cion and parental stock selection, during which, an individual plant without disease and pest is selected as a parental stock and a wattle with the diameter similar to that of the parental stock is selected as a scion; (2) cion cutting, during which, 3 to 6 minutes of the scion is cut as a cion with one bud and one leaf (leafstalk only) left and the section perpendicular to the longitudinal direction of the wattle taken as a grafting surface; (3) parental stock cutting, during which, the parental stock is cut at the grafting height with the section perpendicular to the height direction of the parental stock taken as a grafting surface; (4) cion and parental stock combination, during which, the cion section and the parental stock section are tightly attached to align a cambium; and (5) interface binding and fixing, during which, an interface is fixed preliminarily by an adhesive tape and then the cion is tightly wrapped by a plastic film strip or a small-opening plastic bag so as to be fixed on the parental stock.

The invention relates to an antibacterial biological activity stent and a preparation method thereof. The method comprises the steps that gelatin microspheres are prepared; porous gelatin microspheres are prepared; gelatin microspheres carried with active factors are obtained; an antibacterial stent is prepared, wherein the preparation of the antibacterial stent comprises the steps that at least one of collagen, chitosan and silk fibroin and nano-silver grains are added to acetic acid or malonic acid to prepare a mixed solution, the mixed solution is poured into a die which is horizontally paved with the solid gelatin microspheres, the die is rapidly placed in an ultra cold refrigerator for freezing treatment, and the die is frozen and dried to obtain the antibacterial stent; and the antibacterial biological activity stent is prepared by the steps that the gelatin microspheres carried with the active factors are placed into normal saline to prepare a suspension liquid, and the suspension liquid is poured into the antibacterial stent to obtain the antibacterial biological activity stent after dried at the room temperature. According to the antibacterial biological activity stent and the preparation method thereof, pure natural polymers are taken as raw materials, the nano-silver grains and the bioactive factors are compounded to prepare the stent, so that the stent has the biological activity and antibacterial capacity, can effectively promote vascularization of cambium and can be used for skin injury healing.

The present invention relates to a stem cell derived from cambium of family Gingkoaceae and to method for the isolated culturing thereof. The stem cell derived from cambium of family Gingkoaceae according to the present invention is advantageous as it is isolated at a non-differentiated state without passing through a dedifferentiation process and stably maintained without causing a variation in the cellular growth rate and growth pattern even during a long-term culture, thereby enabling a mass culture. In addition, the stem cell derived from cambium of family Gingkoaceae according to the present invention showed an antioxidant effect similar to or higher than that of existing synthetic antioxidants when the radical-scavenging activity for scavenging radicals generated by the treatment of an oxidant is measured, and thus can be valuably used as an excellent anti-inflammatory composition.

The invention discloses a grafting method of Magnolia wufengensis. A Magnolia denudate nursery-grown plant is taken as a rootstock, a current-growth branch of the Magnolia wufengensis is taken as a scion, and when the branch has a lignifying tendency in autumn, grafting can be started. The grafting method has the following steps: cutting an angular cut reaching xylem closely at the inner side of a phloem portion, then, performing longitudinal vertical sliding cutting downwards from right above the angular cut to the angular cut so as to cut a flat and smooth long tangent plane and ensure that the exposition position of the long tangent plane is mainly disposed on a cambium layer; performing sliding peeling downwards at the opposite side of a scion axillary bud to form a flat and smooth long-horse-ear-shaped tangent plane, wherein the tangent plane bridges over the diameter of the whole branch, a heart center is seen below the middle of the long-horse-ear-shaped tangent plane, and the axillary bud is sheared at a position 0.8 to 1cm away from the upper portion of the axillary bud; and carrying out scion colligation. According to the grafting method provided by the invention, the grafting butt joint surface is enlarged, the more callus a wound generates, healing can be speeded up, through such a means, the technical difficulty of rapid breeding of the Magnolia denudate is solved, and the technical defect is detected that the survival rate by use of a conventional Magnolia denudate grating method is low.

The invention provides a method for grafting fruit tree seedlings in spring. The method comprises the following steps of collecting scions, choosing grafting time, paring rootstock, paring the scions, jointing, cutting the rootstock, erasing sprouts, dressing top, removing bundling substances, and the like. The method is characterized in that the scions are collected at the period of 2 to 3 weeks before germination in spring and are sealed with wax on the bottom on standby; when the rootstock slightly buds, one smooth side exposed to the sun is processed with a downward flat cut with the length of about two centimeters, and the underside of the rootstock is processed with a crisscross cut, so as to form a small oblique plane; a position one centimeter above the scion bud is processed with a downward flat cut with the length of about two centimeters, and then the underside of the scion bud is processed with a crisscross cut, so as to form another small oblique plane; the scions are pressed on the rootstock, the cambiums of the rootstock and the scions are aligned; the rootstock is shorn off at a position two centimeters above the scion bud 10 to 15 days after grafting; when the scion bud germinates, the sprouts of the rootstock are erased; and when a seedling is 20 to 30 centimeters high, the grafting bundling substances are removed. The method solves the problem that the grafting propagation of forest fruits is low in survival rate and high in cost, and is applicable to the grafting propagation of fruit trees.

The invention relates to an asexual propagation method of michelia compressa. The asexual propagation method includes the steps that firstly, seedlings of the michelia compressa are manufactured; secondly, pretreatment of a scion of michelia compressa is performed; thirdly, a grafting method is adopted; fourthly, management after grafting is performed. Liriodendron chinense is used as a rootstock, and the cut-grafting method is used for grafting the michelia compressa. After cutting is sequentially soaked into a plant growth regulator and 1%DMSO, the rootstock is inserted, and after the cambium of the scion and the cambium of the rootstock are aligned, an anti-drop film is used for tightly winding and wrapping a port from bottom to top. According to the asexual propagation method of the michelia compressa, a great number of root-included seedlings of the michelia compressa can be obtained, the rate of reproduction is 81-86 times, and after the seedlings are obtained, the seedlings are grafted on the Liriodendron chinense, namely the rootstock, and the survival rate is 95%. After transplantation on a field is achieved for two years, the tree vigor is strong, a tree is resistant to diseases and capable of wintering safely, the problem of vacancy of the market resource of the michelia compressa is effectively solved, and the asexual propagation method of the michelia compressa is wide in application prospect.

The invention relates to a micrografting method for a tetraploid acacia. The method includes the steps of selecting a seedling of a diploid acacia seed as a rootstock, selecting a tissue culture seedling of the tetraploid acacia as a scion, subjecting the scion and the rootstock to cambium alignment by using a cleft grafting method, twining the interface area with silver paper slips, fixing the silver paper twining area with a grafting clamp, planting the grafted seedling into a potted tray containing matrix, then processing a routine management for 20 - 30 days, and acquiring the plant regeneration. By means of the micrografting method, an integral plant is obtained after cultivating the grafted seedling for 20 - 30 days, and accordingly the high efficiency of rapid planting is achieved. The plant regeneration cultivated by the micrografting method has the advantages of stable genetic character, healthy plant, strong growth, short seedling period, fast plant growing and high survival rate ( with a survival rate over 90% in a field planting production land).

The present invention relates to a composition for preventing or treating cancer, which contains, as an active ingredient, a Taxus cambium- or procambium-derived cell line; a lysate thereof; an extract thereof; or a culture medium thereof. The cell line, the lysate, the extract, and the culture medium has minimized side effects compared to the conventional therapeutic drugs, is safe to the human body, is involved directly in the growth of cancer to induce cancer cell death, and shows anticancer activity of inhibiting angiogenesis occurring in carcinogenesis. Accordingly, the cell line, the lysate, the extract and the culture medium is useful for the prevention, treatment and alleviation of cancer.

The invention discloses a method for improving the graft survival rate of a holly, which comprises the following steps: from the last ten-day of February to the middle ten-day of March and 3 to 5 days before grafting, irrigating a grafting nursery with water once thoroughly; scissoring a twig of a variety to be grafted, which grows robustly with good buds and has a thickness of between 1 and 2 centimeters as a scion, using a grafting knife to cut obliquely once at a 1-centimeter part on the back of a scion bud to cut off 1/3 of a xylem and ensure that the inclined plane is about 2 centimeters in length, then cutting a small inclined plane on the back side of the inclined plane to cut off certain xylem slightly and ensure that the bevel of between 0.5 and 0.8 centimeter in length, and scissoring off the scion at the position of 1 to 2 centimeters above the bud; planting a Chinese holly selectively (without considering the stem height and the thickness), using a lateral branch twig of which the thickness is between 1 and 2 centimeters as a rootstock, using a pair of lopping shears to shear at the position of 5 to 6 centimeters above a bifurcation, selecting a smooth position around the rootstock bark, and cutting vertically downwards for about 2 centimeters at the smooth place with a slight xylem; inserting the scion into a cut of the rootstock so that cambiums on two sides of the long inclined plane of the scion are aligned with and abutted against the cambiums on two sides of the rootstock, and if the scion is thin, the scion is ensured to be aligned with the cambiums on one side of the rootstock; fastening and sealing the cut by a plastic strip at an inserting opening from top to bottom; watering the interface with a watering pot, and sleeving a plastic bag on the cut so as to seal the cut and preserve moisture; building a shading net for sun-shading and temperature reduction at a position 2 meters above the nursery; and removing the plastic bag sleeved outside.

The invention discloses a nursery stock grafting method which comprises the following steps of (1) selection of a stock, selection of a scion, (3) combination, (4) treatment of the combined portion and (5) timely watering after combination. In the combination process, the combined part of the stock is cut open to form a 30-degree oblique plane through a disinfected knife, the phloem of the lower portion of the scion is cut off by 1 cm to 1.2 cm, so that the cambium is exposed, the cambium of the stock is combined with the cambium of the scion. The grafted nursery stock is managed in a conventional method. According to the nursery stock grafting method, the combination area between the cambium of the stock and the cambium of the scion is enlarged, combination is tighter, the grafting survival rate can be increased, the survival rate can achieve 80 percent to 90 percent, the germination rate of the nursery stock after grafting can be increased at the same time, and the nursery stock after grafting can survive ahead of time by 10 days to 15 days.

The invention provides a method for planting dendrobium officinale on a tree, and belongs to the technical field of dendrobium officinale planting. The method comprises the steps of selecting and treating an adnascent tree, pretreating seedlings, treating moss, binding the tree and fixing planting, performing water and fertilizer management after fixing planting, and the like. During treatment on the adnascent tree, a notch which does not damage a stem cambium is formed in a tree trunk, the tree trunk is coated with a pulpous nutrition medium, and the dendrobium officinale is planted after the tree is planted for 3 to 5 days, wherein the used pulpous medium is prepared from dimethomorph, manganese zinc myclobutanil, ivy extracts, gibberellins, andrographolide, polyvinyl acetate emulsion, sorbitol, potassium nitrate, ammonium nitro-humate and monocalcium phosphate. The tree is treated in advance, so that the varieties of the selectable trees can be increased; the dendrobium officinale domesticated seedlings are soaked with planting fixing nutritional liquid before planting fixing and then is irrigated after planting fixing, so that the root system of the domesticated seedlings is developed quickly, plant diseases and insect pests are few, and the survival rate is above 98 percent.

The present invention relates to a cell line derived from the cambium of an herbaceous plant having a storage root and a method for isolating the same. More specifically, relates to a cambium-derived homogeneous cell line having the ability to divide, which is obtained from the cambium-containing storage tissue of an herbaceous plant having a storage root without a separate dedifferentiation process, and to a method for isolating the same. The cell line derived from the cambium of an herbaceous plant having a storage root has active division ability and is homogeneous. Also, it is stable during culture, because it has not undergone a dedifferentiation process. Thus, through the optimization of proliferation thereof, the cell line can be allowed to proliferate in a large amount within a short time. Accordingly, the cell line derived from the cambium of an herbaceous plant having a storage root makes it possible to produce large amounts of useful plants which are difficult to cultivate outdoor due to various problems associated with the period of cultivation, the selection of cultivation land, cultivation cost and the like.

The invention discloses an agarwood promoter and method for producing agarwood in Aquilaria sinensis. It is characterized in that the agarwood promoter is composed of following components in volume percentage: 5-10% of liquid cultivating Schizophyllum commune, 1.5-2% glucose or granulated sugar, 0.5-1% of acetate or malic acid, 85-95% of water. The method comprises the following steps: perforating Aquilaria sinensis trunk at a place not higher than 40cm through the bark, cambium, then perforating the secondary xylem, but hot hurting the primary xylem; introducing the agarwood promoter into the hole using a conduit and agarwood can be formed in half a year. The agarwood promoter of the invention has simple components and is easily acquired and prepared; the operation of the agarwood promoter to promote agarwood production is simple; the method of the invention has many advantages: obvious effect for effectively increasing agarwood productivity, shortening agarwood production period, better utilization and protection for Aquilaria sinensis resources.

The invention discloses a method for cultivating North America holly dwarf products through upper air layering. The method includes the following steps that in the growth and development period of North America holly fruits, a branch which is 20-60 cm in length and about 0.3-1.5 cm in diameter, grows vigorously, is free of plant diseases and pests, and has dense fruits is selected, the 5-10 cm position of the lower portion of the branch is girdled, the girdling width is controlled to range from 0.5 cm to 1.5 cm, a cambium is completely removed, the girdled position is coated with a root inducing agent mixed solution, then the girdled position is coated with a layering medium with the water content ranging from 60% to 80%, finally, the girdled position is wrapped by a plastic film, then an opening and a lower opening are tied, and wetness is kept. 60-70 days later, the medium is full of root systems, the rooting branch is cut off from the wrapping position of the medium, part of leaves are removed, then the rooting branch is transplanted in a culture medium with the ratio of peat to perlite of 3:1(V:V), the medium and the environment are kept wet, the branch is put in shade for seedling recovering for about 20 d, and the survival rate is 96.2% or above. The method has the advantages of being easy and convenient to operate, high in maneuverability, low in cost and high in survival rate, and is an important technical means for achieving dwarfing and miniaturization of North America holly products.

The present invention relates to a plant stem cell derived from the cambium of family Solanaceae, and to a method for isolating and culturing the same. A stem cell derived from the cambium of family Solanaceae, according to the present invention, is useful as it can be isolated in an undifferentiated state without undergoing a dedifferentiation process and stably maintained without causing any variation in the cell growth rate and growth pattern even during long-term culture, thereby enabling large-scale culture. Moreover, the stem cell derived from the cambium of family Solanaceae and the culture thereof, according to the present invention, are useful for preventing and delaying aging as they have the effects of inducing biosynthesis of procollagen and more efficiently inhibiting the generation of enzymes related to aging than retinoic acid, which is known as having an excellent effect in skin wrinkle improvement.

The invention discloses a method for air-layering propagation of a bonsai seedling of bougainvillea spectabilis. The method comprises the following steps: selecting a 4-to-5-year-old appearance-beautiful easily-flexible bough with a diameter of about 3 to 5 cm and a length of about 50 to 100 cm from an 8-to-9-year-old bright-red bougainvillea spectabilis mother plant with a ground diameter of about 6 to 7 cm and no diseases and pests, carrying out girdling, scraping a bark layer and a cambium layer, and compressing a wrapping substrate mixed with a disinfectant and a rooting agent on the position of a cut, carrying out seal packaging by using a plastic film or a longitudinally-cut mineral water bottle, carrying out air layering for about 26 to 37 days, allowing a cut of girdling to grow a root system, allowing the substrate to be filled with the root system in 50 to 60 days, carrying out propagating on the mother plant for 3 to 4 months, carrying out separation with the mother plant, carrying out transferring into a clay pot with an opening diameter of about 25 cm, and placing the clay pot at a shade place for about 15 days, wherein the survival rate is up to 98% or above. The method provided by the invention has less investment, easy operation and significant effects, can rapidly obtain the bonsai seedling of the bougainvillea spectabilis, is rapid in shaping, shortens the period of culturing, and is insusceptible to seasonal restrictions.

The present invention relates to an anti-aging or antioxidant composition which contains, as an active ingredient, a plant stem cell line derived from the cambium of Panax ginseng, including wild ginseng and ginseng, or an extract thereof, a lysate thereof and a culture thereof. The composition has minimized side effects compared to existing anti-aging agents and antioxidants, and thus is safe for the skin. Also, the composition of the present invention shows an antioxidant effect of inhibiting reactive oxygen species caused by exposure to UV radiation that is the major cause of skin aging, and it can effectively reduce or inhibit aging-related factors. Thus, the composition is useful for the prevention and inhibition of aging.

The invention relates to application of ozone oil to an animal infectious disease. Ozone oil is applied to treatment of the animal infectious disease, and is smeared on the focus of the animal infectious disease till the animal infectious disease is cured and cambiums are generated. Ozone oil has strong sterilizing action, slow ozone release and long acting time. Oxygen atoms and grease released by the ozone oil can be used for promoting curing of a wound surface. When the ozone oil is used for treating the animal infectious disease, toxic and side effects on an animal are eliminated, the organs of the animal are not harmed, and a remarkable effect is achieved. According to the invention, the animal infectious disease can be eliminated, the healthy growth and reproduction of animals are promoted, the animal reproduction rate is increased, yield and income are increased for raisers, and virtuous circular development of livestock breeding is promoted.

The invention provides a thermo-sensitive gelatin preparation for endometrium stem cells. The thermo-sensitive gelatin preparation comprises an endometrium stem cell cellular preparation, an endometrium stem cell exosome preparation, chitosan, mannitol, hyaluronic acid, sodium alginate and beta-sodium glycerophosphate. The thermo-sensitive gelatin preparation is free from puncturing and recyclingand only needs to be transmitted into the lumina of an external genital system of women, the thermo-sensitive gelatin is cross-linked to form a semisolid preparation being in complete cooperation withthe shape of the lumina, the repair effect can be achieved, and repeated damage to organisms can be avoided. After being applied, the composite preparation can gradually dissolve through the temperature of human bodies to form a solution, mesenchymal stem cells in the preparation are released, and through polarization, cambium is obtained. Thermo-sensitive gelatincell factors in the preparation can be released slowly, and the defect of massive running off of the cells after recycling is performed can be overcome. The active factors secreted by the stem cells can restrain tissue inflammation,improve the regeneration capacity of tissue and promote repair of original tissue, and can achieve the effect of wound repair.