14 results about "Cambium" patented technology

This invention belongs to the field of rubber tapping technology and discloses a method, system, and medium for generating intelligent latex tapping operation trajectories. The method includes: acquiring X-ray images of living trees in a preset area; performing grayscale conversion and filtering on the X-ray images to obtain processed images; constructing a three-dimensional envelope model of the living tree based on the processed image, the three-dimensional envelope model of the living tree including a bark envelope model and a core-edge envelope model; generating a tapping trajectory based on the bark envelope model and the core-edge envelope model, the tapping trajectory including the bark cutting length, cutting direction, and cutting depth. This invention can accurately identify the boundaries between the latex layer and cambium layer of rubber trees, and then plan its own tapping trajectory through the three-dimensional envelope model of each rubber tree, realizing efficient and accurate intelligent rubber tapping operations.

This invention discloses a method for inducing and culturing burdock stem cells and stem cells, belonging to the field of plant biotechnology. Using burdock roots as explants, the cambium is determined by staining, and the cambium is obtained using enzymatic digestion. The cambium is then inoculated into a specific ratio of induction solid medium and cultured in the dark to efficiently induce primary stem cells. These primary stem cells are then transferred to a proliferation solid medium for subculture and selection to obtain and maintain a vigorous, soft stem cell line. The solid stem cell line is further transferred to a liquid medium for shaking culture to establish a suspension stem cell line. This invention provides high induction efficiency, stable stem cell characteristics, and good reproducibility, offering a high-quality starting material and technical platform for in vitro preservation of burdock germplasm, large-scale production of secondary metabolites, and genetic transformation.

The invention belongs to the technical field of biotechnology and plant tissue culture, and particularly relates to a method for promoting ginsenoside accumulation in ginseng cambium stem cells. According to the method for promoting ginsenoside accumulation in the ginseng cambium stem cells, efficient induction and accumulation of ginsenoside in the ginseng cambium stem cells are promoted by optimizing culture conditions and induction strategies. The ginseng cambium stem cells obtained by the method have high ginsenoside content, and especially saponin components such as Ro, Re, Rg2, Rh1 and the like are remarkably improved.

The application discloses a method and device for grafting artemisia annua to improve yield and quality of chrysanthemum morifolium, and belongs to the technical field of grafting, comprising the following steps: step 1: selecting materials, selecting healthy chrysanthemum morifolium scion as stock, and selecting artemisia annua as rootstock; the rootstock requires thick and strong roots, is free of diseases and pests, and is located on a flat ground with sufficient sunlight and no other obstructions, so as to facilitate subsequent growth and development; step 2: cutting the stock and disinfecting, using a grafting device to cut a grafting incision at the end of the chrysanthemum morifolium scion; the incision requires that the cortex is cut off, and the cambium layer is visible; when the grafting device cannot form at one time, a blade is adjusted, and the length of the cutting is 2-3 cm; the method and device for grafting artemisia annua to improve yield and quality of chrysanthemum morifolium are provided, and are used for solving the problems of low yield and quality of chrysanthemum morifolium products, and the problems of difficulty in cutting the incision of the rootstock during grafting, difficulty in healing the incision after grafting, poor growth quality and low quality.

This invention discloses a method for cultivating hardy kiwifruit, comprising the following steps: S1 Cultivating male 'Jinmi Rootstock No. 1' seedlings; S2 Grafting, starting in mid-to-late February, high-position grafting is performed on the 'Jinmi Rootstock No. 1'; First, a grafting method with a grafting diameter of 0.8 cm is used: the scion is 5-7 cm long, each segment retains 2-3 buds, and the lower end is cut into an oblique surface; the rootstock is cut at a diameter of 0.8 cm, then longitudinally cut, with the cut size similar to that of the scion; then the cambium layers of the scion and rootstock are aligned and tied tightly with a thin film strip. Beneficial effects: 'Jinmi Rootstock No. 1' can increase the average yield of hardy kiwifruit cultivars by 25.7%, showing a significant yield increase; 'Jinmi Rootstock No. 1' has a very well-developed root system, strong vitality, and can be directly propagated by cuttings, making seedling cultivation easy; it improves the adaptability of hardy kiwifruit cultivation and reduces the rate of tree death caused by waterlogging.

The application discloses knockout Populus DOF9 gene in improving wood yield and a method thereof, a DOF9 knockout plant carrier is introduced into a Populus deltoids cv. 'Nanlin 895' to obtain a DOF9 knockout transgenic plant; the stem xylem proportion of the knockout plant is significantly higher than that of a wild type (WT), and the xylem layer number is also significantly increased; the result shows that the stem cambium activity and xylem differentiation of the Populus are promoted after the DOF9 is deleted, and the development and utilization of the Populus have important significance.

The application discloses knockout poplar ACO gene in the application of improving wood yield and a method thereof, an ACO knockout plant carrier is introduced into NL895 to obtain an ACO knockout transgenic plant; the stem xylem proportion in the knockout plant is significantly higher than that of WT, and the xylem layer number is also significantly increased; the result shows that the poplar stem cambium activity and xylem differentiation are promoted after the ACO is deleted, and the poplar development and utilization have important significance.

The application discloses a humidity control and long block bud grafting method for improving the survival rate of ginkgo grafting and special biological active dressing. The method comprises the following steps: pretreating the scion with 50-100 mg / L mannitol solution for 30-60 minutes; cutting a long block bud piece with a thin layer of xylem, the long block bud piece being 3.0-4.0 cm long and 1.5-2.5 cm wide, and ensuring that the cambium is precisely connected; smearing the grafting interface with special dressing containing 0.01%-0.05% indole butyric acid and 0.5%-1.0% carbendazim; and wrapping the grafting interface with a degradable grafting film, and keeping the temperature at 20-28 DEG C and the humidity at greater than or equal to 80% until callus formation. The dressing is composed of 0.01%-0.05% indole butyric acid, 0.5%-1.0% carbendazim and the rest of the base material in percentage by mass. The application solves the problems of water loss, slow healing and susceptibility to infection in ginkgo grafting by combining endogenous water retention, exogenous healing promotion and standard operation, and the survival rate is stably improved to more than 95%, and the optimal survival rate is 98%. The method is simple to operate and suitable for large-scale promotion.

The invention provides an affinity agent for jujube tree twig grafting and a preparation method and application thereof, and relates to the technical field of jujube tree cultivation. The affinity agent is prepared from the following components in mass concentration: 5 to 15g / L of saccharose, 50 to 100mg / L of naphthylacetic acid, 50 to 100mg / L of auxin and 5 to 15mg / L of gibberellin. According to the multi-hormone synergistic jujube tree twig grafting affinity agent provided by the invention, through scientific proportioning of nutrient substances and plant hormones, the viability of stock-scion cambium cells is activated, and rapid formation of calluses is promoted; meanwhile, a set of standardized twig grafting technology system based on the affinity agent is constructed, grafting operation and postoperative management processes are optimized, and efficient healing of rootstocks and scions and robust growth of nursery stocks are achieved. The technology is easy and convenient to operate, the twig grafting technology threshold is lowered, the adaptability of different jujube varieties and stock combinations is improved, and the requirements for large-scale seedling raising and variety renewal are met.

This invention discloses a grafting propagation method for the summer wax plum 'Hongyun'. The method involves applying nitrogen fertilizer, boron fertilizer, and humic acid in the autumn of the previous year to build up energy reserves. In early March of the same year, a compound hormone of GA3+6-BA+NAA is sprayed three times. Combined with black plastic film wrapping to increase temperature and block light, black plastic film mulching to retain moisture, and ridge planting with frequent water management, the cambium layer of the wax plum rootstock and summer wax plum scion branches becomes active and ready for peeling in early spring. At this time, large-block budding is performed, resulting in high scion utilization. The buds sprout in early April, and the scions can be ready for transplanting in November of the same year. This method achieves a grafting survival rate of over 82%, which is more than 16 percentage points higher than traditional summer budding. The transplanting rate in the same year reaches 88%, the seedling cycle is shortened by one year, and the scion utilization rate is increased by 110%. This provides reliable technical support for the large-scale propagation of the rare new variety 'Hongyun'.

The invention discloses a clausena lansium grafting rootstock and scion preparation tool and a clausena lansium grafting method. The preparation tool comprises a sleeve, a blade base and a cutting cutterhead, a baffle is fixed to the front end of the sleeve, and the rear end of the sleeve is sleeved with a holding part. The blade base is installed in the sleeve and can move in the axial direction of the sleeve. The base fixing device is used for fixing the blade base at a set position in the sleeve; the cutting cutterhead comprises a middle blade, a first side blade and a second side blade; one side of the cutting cutterhead is a stock preparation cutting area, and the other side of the cutting cutterhead is a scion preparation cutting area; one end of the cutting cutterhead is fixedly connected with the blade base; the baffle is provided with an open hole for the cutting cutterhead to penetrate through. The length of the cutting cutterhead extending out of the baffle is adjusted by adjusting the position of the blade base in the sleeve. When the preparation tool is used for grafting, the butt joint area and butt joint sufficiency of the cambium can be improved, so that the overall grafting survival rate is increased, and the problem of survival rate fluctuation between different grafting batches is solved.

This invention discloses a method for grafting old kapok trees onto *Ceiba speciosa* rootstock to achieve dwarfing and high yield, relating to the field of Bombacaceae plant cultivation technology. The method includes the following steps: selecting robust, dwarfing *Ceiba speciosa* seedlings with a diameter ≥1.5cm and a height of 60-80cm as rootstock; selecting plump bud segments with a diameter of 0.8-1.2cm from the middle of 1-2 year old branches of superior 10-20 year old kapok trees as scions; and grafting the rootstock 30-40cm above the ground 15-20 days before bud break from late February to early March. Graft at 0cm, align the cambium layers and tie; when the new shoots grow to 10-15cm, untie, remove the lateral buds of the rootstock and erect supports for wind protection; cultivate an open-center crown through trunk setting and heavy winter pruning, controlling the height of mature trees to 2.5-3.5m; apply fertilizer in stages and keep the soil moist; this invention achieves the goal of controllable dwarfing, high yield and quality, early fruiting and preservation of the original edible quality of old kapok by using the core technology combination of grafting local edible old kapok onto kapok rootstock and supporting cultivation management.