6 results about "Cambium" patented technology

This invention belongs to the field of rubber tapping technology and discloses a method, system, and medium for generating intelligent latex tapping operation trajectories. The method includes: acquiring X-ray images of living trees in a preset area; performing grayscale conversion and filtering on the X-ray images to obtain processed images; constructing a three-dimensional envelope model of the living tree based on the processed image, the three-dimensional envelope model of the living tree including a bark envelope model and a core-edge envelope model; generating a tapping trajectory based on the bark envelope model and the core-edge envelope model, the tapping trajectory including the bark cutting length, cutting direction, and cutting depth. This invention can accurately identify the boundaries between the latex layer and cambium layer of rubber trees, and then plan its own tapping trajectory through the three-dimensional envelope model of each rubber tree, realizing efficient and accurate intelligent rubber tapping operations.

This invention discloses a method for inducing and culturing burdock stem cells and stem cells, belonging to the field of plant biotechnology. Using burdock roots as explants, the cambium is determined by staining, and the cambium is obtained using enzymatic digestion. The cambium is then inoculated into a specific ratio of induction solid medium and cultured in the dark to efficiently induce primary stem cells. These primary stem cells are then transferred to a proliferation solid medium for subculture and selection to obtain and maintain a vigorous, soft stem cell line. The solid stem cell line is further transferred to a liquid medium for shaking culture to establish a suspension stem cell line. This invention provides high induction efficiency, stable stem cell characteristics, and good reproducibility, offering a high-quality starting material and technical platform for in vitro preservation of burdock germplasm, large-scale production of secondary metabolites, and genetic transformation.

The application discloses knockout Populus DOF9 gene in improving wood yield and a method thereof, a DOF9 knockout plant carrier is introduced into a Populus deltoids cv. 'Nanlin 895' to obtain a DOF9 knockout transgenic plant; the stem xylem proportion of the knockout plant is significantly higher than that of a wild type (WT), and the xylem layer number is also significantly increased; the result shows that the stem cambium activity and xylem differentiation of the Populus are promoted after the DOF9 is deleted, and the development and utilization of the Populus have important significance.

The application discloses knockout poplar ACO gene in the application of improving wood yield and a method thereof, an ACO knockout plant carrier is introduced into NL895 to obtain an ACO knockout transgenic plant; the stem xylem proportion in the knockout plant is significantly higher than that of WT, and the xylem layer number is also significantly increased; the result shows that the poplar stem cambium activity and xylem differentiation are promoted after the ACO is deleted, and the poplar development and utilization have important significance.

The application discloses a humidity control and long block bud grafting method for improving the survival rate of ginkgo grafting and special biological active dressing. The method comprises the following steps: pretreating the scion with 50-100 mg / L mannitol solution for 30-60 minutes; cutting a long block bud piece with a thin layer of xylem, the long block bud piece being 3.0-4.0 cm long and 1.5-2.5 cm wide, and ensuring that the cambium is precisely connected; smearing the grafting interface with special dressing containing 0.01%-0.05% indole butyric acid and 0.5%-1.0% carbendazim; and wrapping the grafting interface with a degradable grafting film, and keeping the temperature at 20-28 DEG C and the humidity at greater than or equal to 80% until callus formation. The dressing is composed of 0.01%-0.05% indole butyric acid, 0.5%-1.0% carbendazim and the rest of the base material in percentage by mass. The application solves the problems of water loss, slow healing and susceptibility to infection in ginkgo grafting by combining endogenous water retention, exogenous healing promotion and standard operation, and the survival rate is stably improved to more than 95%, and the optimal survival rate is 98%. The method is simple to operate and suitable for large-scale promotion.

This invention discloses a method for grafting old kapok trees onto *Ceiba speciosa* rootstock to achieve dwarfing and high yield, relating to the field of Bombacaceae plant cultivation technology. The method includes the following steps: selecting robust, dwarfing *Ceiba speciosa* seedlings with a diameter ≥1.5cm and a height of 60-80cm as rootstock; selecting plump bud segments with a diameter of 0.8-1.2cm from the middle of 1-2 year old branches of superior 10-20 year old kapok trees as scions; and grafting the rootstock 30-40cm above the ground 15-20 days before bud break from late February to early March. Graft at 0cm, align the cambium layers and tie; when the new shoots grow to 10-15cm, untie, remove the lateral buds of the rootstock and erect supports for wind protection; cultivate an open-center crown through trunk setting and heavy winter pruning, controlling the height of mature trees to 2.5-3.5m; apply fertilizer in stages and keep the soil moist; this invention achieves the goal of controllable dwarfing, high yield and quality, early fruiting and preservation of the original edible quality of old kapok by using the core technology combination of grafting local edible old kapok onto kapok rootstock and supporting cultivation management.