31 results about "Ultra Low Temperature Freezer" patented technology

The invention discloses hydrophobic aerogel containing carboxyl nanocellulose and xanthan gum as well as a preparation method and application of the hydrophobic aerogel. The preparation method comprises the following steps: mixing cellulose and an acidic deep-eutectic solvent, performing water bath treatment, and stirring in distilled water through a wall breaking machine; mixing the nanocellulose suspension and the xanthan gum solution in equal mass, and uniformly stirring to obtain composite hydrogel; pre-freezing the composite hydrogel in an ultralow-temperature refrigerator, and performing vacuum freeze drying through a freeze dryer to obtain composite aerogel; spraying wax emulsion on the obtained composite aerogel, and drying to obtain the hydrophobic aerogel. The invention provides the hydrophobic aerogel containing the carboxyl nano cellulose and the xanthan gum, which is safe and environment-friendly, has good hydrophobic performance, adsorption performance and mechanical performance, can be directly used for food, and can be used for preparing an oil absorption pad and an oil absorption film.

1. The name of the design product: cryopreservation rack. 2. The use of the design product: the design product is used for the cryopreservation rack of the specimen in the ultra-low temperature refrigerator. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view 1.

The invention discloses a preparation method of a blood coagulation factor quality control product. The preparation method comprises the following steps: treating mixed plasma of multiple persons by using a physical and chemical method; and mixing the multi-person mixed plasma with the factor diluent according to a certain proportion to prepare the blood coagulation routine item quality control product with different blood coagulation routine item contents at different concentration levels, subpackaging, and storing in an ultra-low temperature refrigerator at-80 DEG C to obtain the blood coagulation routine item quality control product. The blood coagulation routine item quality control product prepared by the invention is good in product uniformity, stability and frozen product remelting stability, can replace commercial products to be used for quality control of blood coagulation routine item detection, and is beneficial to reducing the detection cost and improving the blood coagulation routine item detection capability.

1. Name of the Design Product: Intelligent Environment Detector (OSD-IWIFI). 2. Use of the Design Product: This design is used for environmental monitoring in cell banks, cryogenic automated storage equipment, liquid nitrogen tanks, ultra-low temperature refrigerators, refrigerators, transfer tanks, thawing instruments, cold storage, etc. 3. Design Key Points of the Design Product: Lies in the shape. 4. Picture or Photograph that Best Illustrates the Design Key Points: Perspective View 1.

The invention provides a gonococcus transport and preservation method, which comprises the following steps: step 1, taking out one strain which is not freeze-dried from an ultralow-temperature refrigerator, placing the strain at room temperature for later use, after the strain is slightly dissolved, taking out a certain amount of bacterial liquid from a biosafety cabinet by using a pipettor, inoculating the bacterial liquid onto a pre-heated culture medium, marking lines in four regions, and then placing the culture medium in an incubator with a candle jar or a CO2 incubator at 36 DEG C for culturing for 36 hours; 2, picking pure bacteria in a biosafety cabinet by using a disposable inoculating loop, inoculating the pure bacteria to a pre-heated culture medium, and placing the culture medium in a 36-DEG C incubator with a candle jar or a CO2 incubator for culturing for 24 hours; and step 3, taking one sterile glass test tube, adding 1mL of sterilized normal saline, scraping bacterial lawn by using a disposable inoculating loop, grinding along the tube wall, putting on a vortex device, sufficiently and uniformly mixing, and adding into sterilized skim milk. The strain is subjected to freeze-drying preservation through a vacuum freeze-drying technology, so that energy expenditure for preservation of gonococcus is saved, and benefits are brought to distribution of national gonococcus interventricular quality assessment substances and transportation of national gonococcus drug-resistant monitoring point specimens.

The ultralow-temperature refrigerator comprises the pulse tube refrigerator, the thermosiphon and an ultralow-temperature refrigerator heat insulation shell, and the pulse tube refrigerator comprises an oil-free linear compressor, a connecting pipe, an after-stage cooler, a heat regenerator, a cold end heat exchanger, a pulse tube, a hot end heat exchanger, an inertia pipe and a pulse tube refrigerator gas reservoir. The thermosiphon comprises a thermosiphon air reservoir, a thermosiphon connecting pipe, a thermosiphon descending section and a thermosiphon ascending section. By utilizing the advantages of environmental protection, energy conservation and no horizontal vibration of the linear opposed pulse tube refrigerating machine, the problems of high energy consumption of a traditional cascade steam compression refrigerating system and unstable vibration operation of other novel refrigerating machines are solved. And the problem of low heat exchange efficiency caused by insufficient heat exchange is solved by utilizing the bent pipeline at the descending section of the thermosiphon. The problem that the ultralow-temperature refrigerator is unstable and unsafe when the refrigerating machine is shut down is solved by utilizing the advantage that the thermosiphon air reservoir is added to relieve overlarge pressure in the thermosiphon when the refrigerating machine is shut down.

The invention provides a mixed refrigerant as well as an additive, a preparation method and application thereof, and belongs to the technical field of refrigerants, the mixed refrigerant comprises the following components in parts by mass: a, 75 parts of combustible components including R290 and R1150; and b, a non-flammable component, the non-flammable component comprises R245fa, R23 and R728, and the non-flammable component is 210 to 265 parts by mass. According to the mixed refrigerant disclosed by the invention, combustible components and incombustible components are reasonably proportioned, and ethylene glycol, ethylene carbonate and propylene carbonate are added as additives, so that the performance of the refrigerant is remarkably improved. The refrigerant has the advantages of being excellent in environmental protection property, low in circulating energy consumption, high in low-temperature performance, good in temperature stability, high in recovery capacity and the like, is suitable for refrigeration equipment such as ultralow-temperature refrigerators, refrigeration houses and air conditioners, and has wide market prospects.

The utility model discloses a biological sample ultra-low temperature refrigerator capable of being placed in a classified mode, which comprises an ultra-low temperature refrigerator main body, the ultra-low temperature refrigerator main body comprises a main box body, a box door is installed on the front surface of the main box body through a hinge, and a sample placing frame is clamped in the main box body. A label placing transparent box is arranged on the surface of the sample placing rack; by designing a clamping assembly, an external thread ring can be rotated in an internal thread groove to fix an air bag body in a classified placement hole, the air bag body is filled with air, and a sample preservation tube penetrates through the air bag body until the sample preservation tube is embedded into the bottom end of the interior of the classified placement hole; and the air bag body deforms to clamp the sample storage tubes in the classified placement holes, so that the air bag body can deform to clamp and place the storage tubes with different sizes conveniently, the placement is more stable, and the classified placement stability of the ultralow-temperature refrigerator main body to the sample storage tubes with different sizes is improved.

The application discloses a kind of composite protein gels and preparation method thereof, its preparation method includes the following steps: with the blood collection tube with anticoagulant is collected blood;The above-mentioned blood is centrifuged at 300-700g centrifugal acceleration at room temperature 10-20min;After centrifugation, blood is transferred to biological safety cabinet, and the upper plasma layer is extracted, and then it is placed in-80 ℃ ultra-low temperature refrigerator and rapidly frozen, and the freezing time is 24-72h;Then, the frozen plasma is taken out, and freeze-drying treatment is carried out under the condition that the temperature is-55-65 ℃ and the pressure is 50-100Pa, and the freeze-dried plasma is prepared;Calcium chloride injection is added to the freeze-dried plasma at a volume ratio of 1:0.5-2, then reacted in a water bath at 60-90 ℃ for 5-15min, and removed to prepare.The protein gel can effectively solve the problem of easy skin stiffening, collapse, high in vivo absorption rate and poor filler stability caused by hyaluronic acid and autologous fat.

A lily pollen dry storage box belongs to the field of agricultural and horticultural technology. The box comprises a C layer, a detachable A layer and / or a B layer arranged on the C layer, and after combination, a two-layer or three-layer storage box body is formed. The A layer and the B layer are both provided with a baffle with a plurality of through holes at the bottom. The A layer is a closed space with a seal, and the bottom is provided with a baffle I with a plurality of through holes I. A drying agent is arranged in the A layer. The B layer is an open box body, and the bottom is provided with a baffle II with a plurality of through holes II for containing fresh medicinal herbs. The C layer is an open box body for containing dried pollen. The bottom of the A layer and the B layer is provided with a detachable sealing connection structure matched with the top of the C layer. The top of the B layer and the top of the C layer have the same structure. Different varieties of lily pollen are separately collected in the plastic box, which is convenient for pollen classification and drying, and can be stored in a 4℃, -18℃ and ultra-low temperature refrigerator for a long time, prolonging the activity of the pollen, and having good airtightness, moisture resistance, spilling resistance, drop resistance and information searching convenience.

The invention relates to the technical field of ultralow-temperature refrigeration equipment, and discloses a refrigeration system. The refrigeration system comprises a refrigerator, a gravity heat pipe system, an air return pipe and a vibration reduction assembly. The refrigerator comprises a cold end; the gravity assisted heat pipe system is communicated with the cold end of the refrigerating machine, the gravity assisted heat pipe system comprises multiple paths of heat pipes arranged on the top and the peripheral side of the inner container, and a working medium collecting area is formed at the bottom of the multiple paths of heat pipes; the inflow end of the air return pipe is communicated with the working medium collecting area; the vibration reduction assembly comprises a first vibration reduction pipe and a second vibration reduction pipe, the first vibration reduction pipe is communicated between the inflow end of the gravity heat pipe system and the cold end of the refrigerator, and the second vibration reduction pipe is communicated between the outflow end of the air return pipe and the cold end of the refrigerator. By means of the arrangement, stable distribution of liquid films on the inner walls of the heat pipes can be guaranteed, meanwhile, it is avoided that backflow of heat exchange working media is blocked due to the fact that the heat pipes have reverse slopes or flat sections, and then the temperature control precision of the ultralow-temperature refrigerator is guaranteed. Meanwhile, the invention further discloses the ultralow-temperature refrigerator.

The invention discloses a calibration method for cryopreservation frame box body taking. The calibration method comprises the steps that S1, an infrared distance meter is correspondingly installed on a mechanical arm of an ultralow-temperature refrigerator; s2, in the designated area, a mechanical arm is moved to the corresponding cryopreservation frame box storing and taking position recorded historically, and the mechanical arm is controlled to move in the vertical direction and the horizontal direction of the irradiation vertical plane of the infrared distance meter; s3, in the moving process of the mechanical arm, the infrared distance measuring instrument synchronously collects infrared distance measuring results, and two sets of distance measuring fluctuations of the two sets of cryopreservation frame box bodies in the vertical direction are obtained correspondingly; and S4, the coordinate height and the translation amount position of the mechanical arm are recorded, and the corresponding box body taking position of the mechanical arm is obtained through calculation and used for controlling the mechanical arm to accurately take the box body. According to the method, the sample box storage positions on the cryopreservation frame are subjected to distance measurement calibration through laser, the sample calibration positions can be determined based on distance measurement data fluctuation, accurate positioning and taking of cryopreservation frame box bodies are achieved, and the dislocation taking influence caused by deformation in the ultralow-temperature environment is avoided.

The invention discloses a clinical test digital intelligent mobile laboratory which comprises a chassis and a compartment. Front wheels and rear wheels are arranged on the chassis, a cab is arranged at the front end of the chassis, and a driving device for driving the front wheels to rotate is installed at the front end of the chassis. The compartment is arranged on the chassis, three partition plates are arranged in the compartment and divide the compartment into a power supply area, a sample storage area, a medicine intravenous dispensing area and a pharmacy area, and side doors are arranged on the two partition plates close to the tail of the compartment. The power supply area is located on the front portion of the compartment and internally provided with a mute generator. The pharmacy area is positioned at the tail part of the carriage; a medical low-temperature box and a medical refrigerator are arranged in the pharmacy area; an ultralow-temperature refrigerator and a centrifugal machine are arranged in the sample storage area; a biological safety cabinet is arranged in the medicine intravenous distribution area; two cabin doors are arranged on the side face of the compartment, one cabin door is used for entering and exiting the power supply area, the other cabin door is used for entering and exiting the sample storage area or the medicine intravenous dispensing area, and a hinged door is arranged at the tail of the compartment. According to the invention, a mobile laboratory is formed, and the efficiency of clinical tests is improved.

The invention provides a step-by-step foaming method for an ultralow-temperature refrigerator based on a low-thermal-conductivity heat insulation plate frame, which comprises the following steps of: (1) preparing a heat insulation frame matched with an inner container by adopting a low-thermal-conductivity material, and enclosing and sealing a foaming space through a positioning structure to ensure that an evaporator is completely accommodated in the frame; (2) after the heat insulation frame is fixed, an LBA foaming material is injected into the frame for foaming, and a primary foaming layer is formed; and (3) sleeving a heat exchanger on the outer side of the heat insulation frame, integrally putting the heat insulation frame into a mold, and injecting a secondary foaming material containing a cross-linking agent into a gap outside the frame for foaming to form a secondary foaming layer. According to the invention, the peripheral space of the evaporator is precisely limited by the low-thermal-conductivity heat insulation plate frame, and a segmented process of primary foaming inside the frame and secondary foaming outside the frame is matched, so that the problems of obvious heat bridge in an evaporator area, non-uniform density of a foaming layer, poor foaming stability of pure LBA and the like in a traditional foaming process are solved; finally, the aims of improving the heat preservation performance of the ultralow-temperature refrigerator, reducing energy consumption, being stable in structure and environment-friendly and controllable in foaming are achieved.

The utility model belongs to the technical field of refrigerators, and particularly relates to a foaming box body for a horizontal ultralow-temperature refrigerator. Comprising a first foaming layer, a second foaming layer and a box shell, the box shell is mounted outside the second foaming layer; the second foaming layer is sleeved on the first foaming layer; the first foaming layer wraps the outer vertical face of the low-temperature lining, a fixing assembly is arranged in the first foaming layer, one side of the fixing assembly is detachably connected with the outer vertical face of the low-temperature lining, and the other side of the fixing assembly is fixedly connected with the tubular heat exchanger. The foaming box body is simple in structure and easy to manufacture, the installation precision of the tubular heat exchanger is improved, and the yield of the foaming box body is improved.

The utility model relates to an air inlet valve for air balance, an air balance device and an ultralow-temperature refrigerator, and belongs to the technical field of refrigeration equipment. The air inlet valve comprises a valve body and a plugging piece. A valve cavity is formed in the valve body, and an air inlet and an air outlet connector are formed in the valve body. A guide part is arranged in the air inlet and divides the air inlet into a guide channel located in the guide part and an air inlet channel located between the guide part and the air inlet; the plugging piece is arranged in the valve cavity and comprises a sealing part and a guide part; the sealing part floats up and down in the valve cavity under the action of airflow flowing in from the air inlet channel, and falls to cover the air inlet to seal the air inlet channel without the action of airflow; the guide part is arranged in the guide channel and is in sliding fit with the guide channel; the top end of the guide part is connected to the bottom of the sealing part to move up and down along with the sealing part; the air inlet valve adopts the plugging piece with a novel structure and is provided with the guide structure for guiding the plugging piece to fall, and an air inlet with a relatively large size can be designed so as to meet the requirement of rapidly balancing air pressure.

The invention discloses a method for screening Yili horse mammary gland development key genes and application thereof, and belongs to the field of genomics and molecular breeding. The method comprises the following steps: step 1, selection and treatment of experimental Yili horses: slaughtering the Yili horses in the early lactation period and the peak lactation period, collecting breast tissue samples, putting the breast tissue samples into a cryopreservation tube, putting the cryopreservation tube into liquid nitrogen, taking the cryopreservation tube back to a laboratory, and putting the cryopreservation tube into an ultralow-temperature refrigerator; step 2, carrying out RNA extraction on the Ili horse mammary gland tissue, constructing an ATAC library, an RNA library, a CUT-Tag library and an STRIPE library, and carrying out sequencing; and step 3, carrying out conjoint analysis on the sequencing data in the step 2, and mining key genes related to lactation. The invention also provides application of the method in a horse variety breeding process. The Yili horse mammary gland development and lactation related genes are excavated through a multi-omics technology, the blank in the prior art is filled, a target spot is provided for molecular marker-assisted breeding, and breeding of high-yield and high-quality horse varieties is accelerated.

Disclosed herein are methods of making an implant material, the method involving harvesting allograft cancellous bone containing bone marrow from a donor within 24 hours of death; incubating the harvested allograft cancellous bone in an ultra-low freezer within 144 hours of death for at least 5 days; and then selectively lysing cells in the cancellous bone. This results in cancellous bone that is enriched for cells resistant to lysing, such as mesenchymal cells as well as a lysate containing factors (e.g. growth factors) released from the lysed cells. This lystate can be administered to cortical bone, e.g. demineralized cortical bone, with hydrochloric acid (HCI) or glycolytic acid so that these factors attach to the demineralized bone.

The utility model discloses a cell programmed cooling instrument suitable for biological cell programmed cooling, and relates to the technical field of cell cryopreservation, the cell programmed cooling instrument suitable for biological cell programmed cooling comprises a box body and a cover body, the cover body is rotatably covered on the upper surface of the box body; a fixing base used for fixing the discharging device is arranged in the box body, and the discharging device is located on the fixing base in a separable mode. Supporting columns for supporting are arranged on the lower surface of the box body; a temperature detection probe, a fan, a power supply and a control panel are arranged on the box body, and the temperature detection probe, the fan and the power supply are electrically connected with the control panel; the fan generates cavity negative pressure to drive external cold air to flow so that programmed cooling can be conducted in the ultralow-temperature refrigerator without liquid nitrogen, and the temperature detection probe and the control panel are adopted for monitoring the temperature and controlling the cooling process. The method is suitable for various cells needing programmed cooling, and cooling programs can be conveniently set.

The utility model relates to the technical field of ultra-low temperature refrigerators, in particular to an ultra-low temperature refrigerator with adjustable partition plate spacing, which comprises a low-temperature refrigerator, mounting blocks are fixedly arranged on the left side and the right side in the low-temperature refrigerator, mounting grooves are formed in the mounting blocks, a partition plate is arranged between the two mounting blocks, and the partition plate is clamped into the mounting grooves. A reinforcing clamping block is arranged at the front end of the mounting block, a movable groove is formed in the reinforcing clamping block, a guide rod is arranged in the movable groove, clamping plates are arranged at the upper end and the lower end in the mounting groove, and a guide block slidably connected to the outer portion of the guide rod in a sleeving mode is arranged on one side of each clamping plate. A spring sleeving the guide rod is arranged at one end of the guide block, a clamping block is fixedly arranged on the lower end face of the clamping plate, clamping grooves are formed in the upper end and the lower end of the interior of the partition plate, the clamping block is clamped into the clamping grooves, and a reinforcing plate is arranged below the partition plate. The partition plate can be adjusted, meanwhile, the bearing capacity of the partition plate is enhanced, and the use efficiency of the partition plate is improved.

The invention relates to a purification method of ethyoxyl pentafluorocyclotriphosphazene. The purification method comprises the following specific steps: S1, preparing an organic solvent and ethyoxyl pentafluorocyclotriphosphazene into an ethyoxyl solution; s2, the prepared ethyoxyl solution is put into an ultralow-temperature refrigerator at the temperature ranging from-80 DEG C to 0 DEG C for freezing crystallization, and the freezing time ranges from 1 h to 8 h; s3, the frozen ethyoxyl solution is subjected to solid-liquid separation, and solid-state ethyoxyl pentafluorocyclotriphosphazene is obtained; s4, the separated solid ethoxypentafluorocyclotriphosphazene is melted at the room temperature, the melted ethoxypentafluorocyclotriphosphazene is subjected to the sweating process by repeating the steps S1 to S3, and the purity of the ethoxypentafluorocyclotriphosphazene reaches 98% or above. The method can effectively remove impurities in the ethoxypentafluorocyclotriphosphazene, improves the purity of the ethoxypentafluorocyclotriphosphazene, and is simple to operate, efficient, low in energy consumption, economical and environment-friendly.

The present invention discloses a portable mobile Stirling ultra-low temperature thermostat, comprising a box body, wherein a refrigeration chamber and a control chamber are arranged side by side inside the box body; a Stirling refrigerator is installed on a support frame inside the control chamber, a heat preservation cold head is installed on the top of the Stirling refrigerator, and a cold guide pipe connected to the heat preservation cold head is arranged around the outside of the refrigeration chamber; a heat insulation structure is arranged between the refrigeration chamber and the inner wall of the box body; a box cover is arranged on the upper side of the refrigeration chamber; a connecting pipe is transversely installed in the side wall of one side of the box body, and one end of the connecting pipe extends to the inside of the refrigeration chamber; a sealing plug is detachably installed at the outward end of the connecting pipe to seal the connecting pipe; a temperature probe is used to insert into the connecting pipe after the sealing plug is removed from the connecting pipe and extend to the refrigeration chamber for temperature monitoring. The present invention can realize ultra-low temperature constant temperature control, is convenient to transport and carry, and solves the technical problem that the existing ultra-low temperature refrigerator cannot be transported.

The utility model relates to the technical field of ultralow-temperature refrigerators, in particular to an energy-saving ultralow-temperature refrigerator. According to the technical scheme, the energy-saving ultralow-temperature refrigerator comprises a refrigerator body and further comprises a sealing door, an ultralow-temperature low-energy-consumption drawer assembly and a low-loss cold water assembly. A sealing door is arranged at the front end of the refrigerator and connected with the refrigerator through a hinge. A low-loss cold water assembly is arranged in the sealing door; an ultralow-temperature low-energy-consumption drawer assembly is arranged in the refrigerator, and the ultralow-temperature low-energy-consumption drawer assembly is slidably connected with the inner wall of the refrigerator. Refrigerant is conducted to the heat absorption plate through the low-temperature-resistant refrigerant pipe belt in the ultralow-temperature low-energy-consumption drawer assembly, so that the heat absorption capacity of the drawer box is effectively improved, the cooling performance is further improved, and the problems that due to the fact that a freezing heat absorption structure of an existing ultralow-temperature refrigerator is usually arranged on the inner wall of the refrigerator, the heat absorption efficiency in a drawer type container is poor, and the refrigerating efficiency is poor are solved. And serious energy waste can be caused by frequent opening and closing of the refrigerator door.

The present application relates to the engineering of biomaterials for cell culture, in particular to a method for enhancing the freeze-drying stability of porous microcarriers. The method comprises the following steps: (1) placing the porous microcarriers which have been sufficiently swollen in water, until the liquid surface is about to contact the porous microcarriers; (2) soaking the porous microcarriers obtained in step (1) in an organic solvent for dehydration treatment; (3) using a Pasteur dropper, a pipette or an electric pipette, transferring the dehydrated porous microcarriers in the container into a tray, sealing the top of the tray and placing it in liquid nitrogen or an ultra-low temperature freezer for pre-freezing; (4) placing the pre-frozen porous microcarriers in a freeze-drying box for freeze-drying. The method can effectively solve the problem of shrinkage, collapse and structural damage of the porous microcarrier scaffold during freeze-drying, and has the advantages of short drying period, low energy consumption and suitability for large-scale production of porous microcarriers.

The utility model discloses a stem cell low-temperature preservation device which comprises an ultralow-temperature refrigerator, the upper end of the ultralow-temperature refrigerator is fixedly connected with a refrigerating chamber, the bottom of the refrigerating chamber is fixedly connected with a supporting seat, a clamping groove is formed in the middle end of the top of the supporting seat, and containing grooves are formed in the two ends of the supporting seat. The two ends of the containing groove are movably connected with guide wheels through bearings, and the surfaces of the guide wheels are movably connected with supporting sliding rods. Through the arrangement of the containing table, stem cell samples needing to be stored at low temperature can be contained in a refrigerating chamber of the ultralow-temperature refrigerator, through the arrangement of the containing groove, the guide wheel and the supporting sliding rod, supporting and guiding can be conducted between the supporting base and the two ends of the containing table, and meanwhile through the arrangement of the fixing shell, the clamping rod and the clamping groove, the stem cell samples can be stored at low temperature. The containing table and the supporting base can be clamped and limited, and the situation that the containing table is displaced due to stress in the low-temperature preservation process of the stem cell samples is avoided.

1. The name of the design product: under-counter ultra-low temperature refrigerator. 2. The use of the design product: for the low-temperature storage of medical products such as vaccines, blood, reagents, and medicines, for scientific research institutes, biological engineering, blood stations, hospitals, disease prevention and control, laboratories, etc. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view.

The invention belongs to the technical field of biological sample storage, and particularly relates to a vertical ultralow-temperature refrigerator with a frost breaking structure and a frost breaking method of the vertical ultralow-temperature refrigerator. The device comprises a refrigerator body, at least one pair of supports are arranged in the refrigerator body, the ends, facing the interior of the refrigerator body, of the supports are hinged to the refrigerator body, and partition plates are suitable for being placed above the supports; the blocking base and the positioning assembly are arranged in a split mode, the blocking base is installed at the end, away from the hinged position, of the support, and the positioning assembly is elastically arranged in the refrigerator body; wherein one end of the positioning assembly is detachably and fixedly connected with the blocking seat. The support is positioned or opened through the separating base and the positioning assembly which are arranged in a split mode so as to judge whether the support and the partition plate are frosted or not, and the partition plate can be conveniently inserted and taken out through the movable support. The positioning assembly is elastically arranged, so that the positioning assembly can conveniently break frost through elastic force or thrust.