144 results about "Ultra Low Temperature Freezer" patented technology

An automated storage and retrieval system stores containers, typically containing biological or chemical samples, at ultra-low temperatures, i.e., from about −50° C. to about −90° C., preferably about −80° C. under normal operating conditions. Dry gas air flows are used to reduce moisture and the consequential frost within the freezer compartment. A custom insulated door is provided with an access module and a tube picking compartment as well as servo motors for controlling a robot within the ultra-low temperature freezer compartment. The robot automatically places sample storage containers in stationary storage racks within the freezer compartment. Magnetic couplings are used to transmit mechanical power from outside of the freezer compartment to the robot inside of the freezer compartment. The robot has a simplified mechanical configuration. The custom door can be readily attached to standard freezer bodies.

The invention relates to an ultra-low temperature preservation method for oil palm pollen, which comprises the following steps of: drying the collected fresh oil palm pollen after impurity removal to control the residual moisture content of the oil palm pollen to between 8 and 9 percent; filling the dried oil palm pollen into a freezing pipe, directly putting the freezing pipe into liquid nitrogen to perform pre-freezing treatment, then transferring the freezing pipe to an ultra-low temperature refrigerator for preservation, and flushing the ultra-low temperature preserved oil palm pollen by using normal-temperature tap water during de-freezing. The method has simple process and low cost, effectively realizes long-term preservation of the oil palm pollen by preserving the oil palm pollen at the ultra-low temperature on the premise of controlling the residual moisture content of the pollen, and has the advantages of low preservation cost, long storage time, high pollen germination rateand the like.

The invention relates to a method for preparing a graphene and inorganic salt high-temperature phase change composite material. The method comprises the following steps of: (1) preparing graphene sol; (2) preparing an inorganic salt solution, adding a surfactant, and mixing uniformly; (3) adding the graphene sol into the inorganic salt solution, and stirring until the graphene sol and the inorganic salt solution are mixed uniformly to obtain a composite material preform; (4) putting the composite material preform into an ultra-low-temperature freezer, quickly freezing to form a solid and taking out; (5) putting the solid-state composite material preform into a vacuum freezing dispersion drying instrument and drying; and (6) taking out after the drying of the composite material preform is finished, and calcining until the moisture is completely removed to obtain a grapheme/inorganic salt phase change composite material. Compared with the prior art, the method has the advantages that: the process is reasonable, easy to operate and low in cost; and the prepared grapheme/inorganic salt phase change composite material has the advantages of high coefficient of heat conductivity, high energy storage density and the like, has excellent performance, can meet different application requirements, and is suitable for industrialized production.

An ultra low temperature freezer is optimized with a combination of vacuum and fiberglass insulation for long-term biological storage with accurate process cooling with critical temperature performance. A programmable cooling and cryogenic freezing system uses sealed liquid nitrogen for cooling and freezing. A hybrid completely non-mechanical system exhibits temperature uniformity and reliability, saves space, requires extremely low operating energy and minimizes need for air conditioning in the operating environment. Top-located components control the flow of liquid nitrogen even under flooding conditions. Sectioned inner doors mitigate thermal transfer to other samples and maintain ULT while accessing the freezer.

The invention provides a method for preparing a human stem cell sourcing biobeauty raw material. The method includes the steps of obtaining protein suspension from a culture solution of human stem cells and human stem cell lysis, after hyperconcentration, preparing into human stem cell essence which is rich in cell growth components and has the efficacy of skin regeneration and repairing; performing further freeze drying, and preparing into freeze-dried powder capable of being stored in a -80 DEG C ultra low temperature refrigerator for a long time to serve as a biobeauty raw material for skin regeneration and repairing. The biobeauty raw material can be added to face cream, body lotion and toner to prepare regeneration and repairing face cream products, or added to facial masks to prepare regeneration and repairing facial mask products, or directly prepared into regeneration and repairing essence products, which is applicable to beauty of skin wrinkles, hyperpigmentation and scars.

An ultra low temperature freezer evaporator (ULT) apparatus that has a triple feed capillary tube system. The ULT apparatus prevents the low stage compressor from running in a vacuum. The invention significantly reduces the “pull down” time. Further, the ULT freezer evaporator apparatus significantly increases the transfer area between the evaporator tubing and the contact surface on the unit to assist the transfer of heat from the refrigerated unit through the condenser to the surrounding room.

The invention relates to an antibacterial biological activity stent and a preparation method thereof. The method comprises the steps that gelatin microspheres are prepared; porous gelatin microspheres are prepared; gelatin microspheres carried with active factors are obtained; an antibacterial stent is prepared, wherein the preparation of the antibacterial stent comprises the steps that at least one of collagen, chitosan and silk fibroin and nano-silver grains are added to acetic acid or malonic acid to prepare a mixed solution, the mixed solution is poured into a die which is horizontally paved with the solid gelatin microspheres, the die is rapidly placed in an ultra cold refrigerator for freezing treatment, and the die is frozen and dried to obtain the antibacterial stent; and the antibacterial biological activity stent is prepared by the steps that the gelatin microspheres carried with the active factors are placed into normal saline to prepare a suspension liquid, and the suspension liquid is poured into the antibacterial stent to obtain the antibacterial biological activity stent after dried at the room temperature. According to the antibacterial biological activity stent and the preparation method thereof, pure natural polymers are taken as raw materials, the nano-silver grains and the bioactive factors are compounded to prepare the stent, so that the stent has the biological activity and antibacterial capacity, can effectively promote vascularization of cambium and can be used for skin injury healing.

The invention relates to a heat pipe heat transfer system at a low-temperature end of a low-temperature refrigerator. The heat pipe heat transfer system comprises a cold source and a heat pipe heat transfer module, wherein the cold source is used for supplying cold, and the heat pipe heat transfer module is used for transferring the cold into the low-temperature refrigerator; the heat pipe heat transfer module comprises a heat exchange block connected with the cold source; the heat exchange block is also connected with at least one heat pipe; the at least one heat pipe is arranged on a low-temperature refrigerator liner, and low-temperature cycle working fluid fills the at least one heat pipe. The structure size of the heat transfer system is reduced, and the weight of the heat transfer system is reduced; by adopting a modular design mode, the quality consistency in volume production is guaranteed; the large-scale production of an ultra-low temperature refrigerator adopting a novel low-temperature refrigerating machine is favorably realized; the efficient heat transfer bottleneck problem between the compact refrigerating end of the novel low-temperature refrigerating machine and the high-volume refrigerating space of the low-temperature refrigerator is solved; the cold of the novel low-temperature refrigerating machine is rapidly and efficiently transferred to the low-temperature refrigerator; compared with the traditional refrigerating system, the heat pipe heat transfer system has the advantages that the problem lubricating oil is hard to return at low temperature is eliminated, and the phenomenon that the refrigerating system is blocked due to the fact that the lubricating oil condenses is avoided.

In an ultra-low temperature freezer (R) using refrigerant mixture in which plural kinds of refrigerants having different boiling points are mixed, in order to ensure the flow rate of liquid refrigerant into a supercooler (31) and enhance the cooling efficiency of a cryocoil (32), the ultra-low temperature freezer (R) comprises: a main refrigerant circuit (38) provided with the cryocoil (32) and a capillary tube (29); and a sub refrigerant circuit (39) that is connected at the upstream end to the upstream end of the main refrigerant circuit (38) to branch off therefrom and provided with a capillary tube (28), and the ultra-low temperature freezer (R) is configured so that the sub refrigerant circuit (39) is lower in height than the main refrigerant circuit (38). Thus, gas-liquid mixture refrigerant discharged from the primary side (31a) of the supercooler (31) flows into the sub refrigerant circuit (39) at a higher rate than into the main refrigerant circuit (38) and, in turn, liquid refrigerant flows more into the sub refrigerant circuit (39) than into the main refrigerant circuit (38).

The invention relates to a method for extracting sulforaphane from leaves of cauliflowers, which comprises the following steps of: after bulbs of the cauliflowers are harvested, immediately collecting waste leaves, cleaning, and performing freezing treatment in an ultralow temperature refrigerator; performing vacuum freeze drying, uniformly crushing into powder, taking 1 part of powder, adding exogenous myrosinase in an amount which is 6 to 10 times weight of the powder and deionized water in an amount which is 4 to 6 times weight of the powder, and fully mixing into uniform slurry; and performing enzymolysis reaction, solvent extraction, filtration, concentration, and vacuum freeze drying to obtain high-purity sulforaphane. The raw material sources are rich, the extraction method is simple, and the method is more suitable for industrial production.

The invention discloses a litchi pollen collecting and storing method, which belongs to the technical field of fruit tree pollination. The method comprises the following steps: 1, collecting litchi anthers which are matured but not cracked at a developmental stage and putting the collected litchi anthers into a glass culture dish; 2, putting the glass culture dish in which the litchi anthers are placed into a drying box and drying the litchi anthers in the glass culture dish by using the drying box; 3, pouring the dried litchi anthers and the scattered pollen into a stainless steel screen, and putting the pollen into a centrifuge tube after enabling the pollen to pass through the stainless steel screen; and 4, sealing the pollen in the centrifuge tube and storing the pollen in an ultra-low temperature refrigerator at -86 DEG C. The method is simple, convenient and easy to implement, and can be used for performing a collecting operation even under different weather conditions. The obtained pollen is high in germination rate and can be stored for the longest time.

A preparation method for sweet orange essence microcapsule relates to a preparation technology of essence microcapsule. A preparation method for sweet orange essence microcapsule is provided with simple technology, safe and controlled operation, collapse and excellent releasing performance microcapsule wall film. The preparation steps comprise bean separation albumen aqueous solution and Arabic gum aqueous solution is prepared, an emulsion is obtainer by putting sweet orange essence into bean separation albumen aqueous solution and homogenizing, a wet microcapsule is obtained by putting and mixing Arabic gum aqueous solution into emulsion and putting alcaine or acetic acid to adjust PH to 4.0. The reinforced wet microcapsule is obtained by cooling the wet microcapsule down to 0 to 15 DEG C and dextrose being putting into the solution concomitant mixing. The powder-shaped sweet orange essence microcapsule can be obtained by putting the reinforced wet microcapsule into ultra low temperature ice box and drying the reinforced wet microcapsule in vacuum freeze drier.

The invention discloses a solid phase extraction-liquid chromatography-mass spectrometric detection method of glyphosate in aquatic products. The method has the advantages that ultrasonic-assisted ultrapure water is mixed with dichloromethane to extract the glyphosate in the aquatic products, time consumption is reduced, and good extraction effect and simpleness in operation are achieved; a C18 solid phase extraction column is used to perform enrichment and purification, a good purification effect is achieved, and interference of impurities in extract on final instrument measurement is lowered effectively; a liquid chromatography-mass spectrometry method is used to perform multi-reaction monitoring, and high sensitivity and accuracy are achieved; compared with the existing testing technology, the method does need derivation during glyphosate detection, derivatization reagents and equipment such as high-temperature water bath and oil bath pots or an ultra-low-temperature refrigerator are not needed, the method can perform qualitative and quantitative measurement at the same time and is simple to operate, practicable, low in consumption cost and capable of fast analyzing the glyphosate in the aquatic products.

The invention discloses a cryopreservation method for a tissue block used for cell culture. The method includes: firstly performing homogenate treatment on a tissue into a chyle state, then adding a refrigerating fluid and mixing them uniformly, subpackaging the mixture into a freezing tube containing sucrose, DMSO and glutathione, then placing the freezing tube in a cryopreservation box and letting the box stay overnight in a -80DEG ultra low temperature refrigerator, and finally putting the freezing tube into liquid nitrogen for long-term preservation. During unfreezing, centrifugal washing by an unfreezing solution and a culture solution is performed in order, then the tissue block can be directly subjected to inoculated culture or can be digested into a single cell and is then subjected to culture. The method provided in the invention firstly employs a homogenizer to treat the tissue into a chyle state, thus facilitating permeation of a cryoprotectant into tissue cells and removal of intracellular water. At the same time, the impermeable protective agent sucrose added in the refrigerating fluid is also conducive to removal the water from the tissue cells. The adding of the antioxidant substance glutathione helps alleviate the damage of oxygen free radicals on intracellular protein. After unfreezing, the tissue block is directly inoculated in a culture bottle, and the adherent survival rate is over 96%.

The invention discloses a Botrytis cinerea metabolome sample pretreatment method, and a metabolome detection method based on gas chromatography-mass spectrometry (GC-MS). The Botrytis cinerea metabolome sample pretreatment method comprises the following steps: culturing Botrytis cinerea on a PDA plate paved with glass paper for 2-4d (preferably 3d), collecting mycelia, inactivating the mycelia by liquid nitrogen, and preserving the inactivated mycelia in an ultralow temperature freezer; carrying out freeze grinding crushing on the obtained mycelium sample, extracting metabolome, and carrying out enzyme inactivation by adopting a frozen methanol-water mixture; and centrifuging, drying, and carrying out an oximation (or hydrazone formation) and silylation two-step derivatization reaction to obtain the pretreated sample for GC-MS detection. The invention also provides the sample detection method through GC-MS. The detection method is carried out under the following detection conditions: a HP-5MS capillary column (30m*0.25mm*0.25[u]m), a sample size of 1[mu]L, programmed heating, an ion source temperature of 230DEG C and a full scanning range of m/z 20-650. Metabolome detection results with good reappearance can be obtained through the extraction and detection methods.

The invention provides a preparing method of immune-cell-derived biological-beautifying raw materials. The method includes the steps that a culturing solution for culturing immune cells and a protein suspension obtained by splitting immune cells are subjected to hyperconcentration to obtain immune-cell essence, wherein the essence is rich in anti-inflammatory and anti-oxidation ingredients and has the anti-inflammatory and anti-oxidation effects; the essence is further subjected to freezing drying to obtain freezing-dried powder which can be stored in a -80-DEG-C ultra-low-temperature refrigerator for a long time, and the anti-inflammatory and anti-oxidation skin biological-beautifying raw materials are obtained. When the biological-beautifying raw materials are added into face cream, skin cream and toner, anti-inflammatory and anti-oxidation face cream products can be prepared; when the biological-beautifying raw materials are added into masks, anti-inflammatory and anti-oxidation mask products can be prepared; the biological-beautifying raw materials can be directly prepared into anti-inflammatory and anti-oxidation essence products and can be used for treating skin acne, removing freckles, blocking the sun, resisting oxidation and the like.

The invention relates to a preparation method of a lightweight high-strength heat-insulating mullite fiber porous ceramic. Deionized water, polyacrylamide, boron carbide powder and silica sol are mixed and placed in a planetary ball mill for ball milling to obtain evenly-mixed binder slurry; chopped polycrystalline mullite fiber is added to the binder slurry and stirred to obtain fiber slurry; thefiber slurry is poured into a mold having a filter screen at the lower end, and filtered by suction by a vacuum pump to obtain a fiber porous ceramic wet body; after the fiber porous ceramic wet bodyis demolded, the fiber porous ceramic wet body is placed in an ultra-low temperature refrigerator for freezing; and the completely-frozen body is placed in a freeze dryer for freeze-drying to obtaina mullite fiber porous ceramic dry body, and the dried body is sintered in a high temperature furnace to obtain the mullite fiber porous ceramic. The preparation method overcomes the problems that thefiber porous ceramic has high density, low porosity and low compressive strength due to uneven bond distribution. A porous ceramic binder is evenly distributed at fiber nodes to support and fix fiber.

The invention discloses an ultra-low temperature freezer, which comprises a shell, a liner, an outer door and an inner door. The inner door and the outer door adopt foamed solid door bodies. At least two sealing strips are arranged on the outer door and the inner door. The mouth of the liner is connected with the shell through a freezer mouth connecting structure. Reinforcing iron is embedded into the freezer mouth connecting structure. The freezer mouth connecting structure is made of a nonmetallic material. Since the inner door is made of polyester in a foaming way and the internal part of the inner door is solid, the inner door is not deformed after the product refrigerates and the cold loss is fewer; since at least two sealing strips are arranged on each of the inner door and the outer door, the entire door body is provided with at least four sealing strips to multilaterally seal the freezer and the cold loss is limited to the utmost extent; and since the freezer mouth connecting structure made of the nonmetallic material is arranged between the shell and the liner, metal connection does not exist between the shell and the liner, the cold bridge is cut off, the cold in the freezer is effectively prevented from being lost through heat conduction of metal and the energy consumption is reduced. The ultra-low temperature freezer has the advantages of simple structure, high cold insulating performance, small cold conduction coefficient, small cold loss and good cold storage effect.

A storage cabinet (16) is provided for an ultra-low temperature freezer (10). The cabinet (16) includes a base platform (62a), a plurality of side structural insulated panels (45, 50, 55) each defining a side wall of the storage cabinet (16), and a plurality of generally vertically oriented posts (40) extending from the base platform (62a). Each of the plurality of vertically oriented posts (40) has a slot (40a) for receiving an edge portion (45a, 50a, 55a) of one of the insulated panels (45, 50, 55) therealong. The slot (40a) may have a generally U-shaped profile that surrounds the edge portion (45a, 50a, 55a) of one of the insulated panels (45, 50, 55). At least one of the generally vertically oriented posts (40) has a channel (40c) that extends along a longitudinal dimension thereof, the channel (40c) being configured to receive one of insulation, tubing, or wiring of the freezer therethrough.

The invention discloses a large-size MXene nanosheet rich in oxygen functional groups as well as a preparation method and an application thereof, and relates to an MXene nanosheet as well as a preparation method and an application of the MXene nanosheet. The invention aims to solve the problem that the transverse size of the MXene nanosheet prepared by the existing method is small. The large-sizeMXene nanosheet being rich in oxygen functional groups is formed by stacking a plurality of few-layer MXene nanosheets. The preparation method comprises the following steps: 1, centrifuging a two-dimensional MXene nanosheet suspension to obtain a muddy precipitate; 2, adding deionized water into the muddy precipitate, stirring the slurry, freezing the slurry in an ultra-low temperature refrigerator at -80 DEG C, and finally freeze-drying in a freeze dryer to obtain a freeze-dried product; and 3, placing the freeze-dried product into a solvent for carrying out ultrasonic treatment. The invention discloses applications of the large-size MXene nanosheet as an electrode material of a supercapacitor or a lithium ion battery. According to the preparation method, the large-size MXene nanosheet rich in oxygen functional groups can be obtained.

The invention discloses a cellulose-based three-dimensional porous composite material supporting nano-zinc oxide and a preparation method thereof. The preparation method includes the steps of: 1) according to weight percentage ratio, successively dispersing 40-60% of a cellulose-[beta]-cyclodextrin composite material, 40-60% of PAMAM-modified konjac, and 1-5% of a pore-forming agent in deionized water, bath ratio being 1:50, performing ultrasonic mixing to uniform, and performing stirring reaction at normal temperature for 24 h at constant speed; 2) pouring a reaction product into a mould, placing the mould in liquid nitrogen for 1 min, taking the mould out, pre-freezing the reaction product in a refrigerator for 6 h at -20 DEG C, freezing the reaction product in a Thermo ultra-low temperature refrigerator for 6 h at -80 DEG C, and processing the reaction product in a freeze-drying machine for 48 h to obtain the cellulose/konjac/cyclodextrin three-dimensional porous composite material;and 3) with electrostatic effect as film forming driving force, performing layer by layer self-assembly on the surface of the three-dimensional porous composite material with the nano-zinc oxide. Inthe invention, with the konjac, cellulose, beta-cyclodextrin and zinc oxide as the raw materials, the method has sufficient raw materials and low cost. The material has important application value inthe fields such as photocatalysis, photoelectric materials, adsorption materials, biomaterials, etc.