Plant stem cell derived from cambium of family gingkoaceae and method for isolation thereof

A ginkgoaceae and cambium technology, applied in the field of stem cells and their separation, can solve problems such as variation, high production costs, and complex chemical structures

Active Publication Date: 2012-05-16
深圳露泉实业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

First, the content of physiologically active substances in plants is extremely limited
Second, plants grow very slowly
Fourth, it involves the environmental problem known as destruction of nature
Fifth, in the case of plant-derived physiologically active substances, the chemical structure is

Method used

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  • Plant stem cell derived from cambium of family gingkoaceae and method for isolation thereof
  • Plant stem cell derived from cambium of family gingkoaceae and method for isolation thereof
  • Plant stem cell derived from cambium of family gingkoaceae and method for isolation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] [Example 1: Preparation of stem cells derived from cambium of Ginkgoaceae]

[0066] 【1-1: Preparation of plant materials】

[0067] Ginkgo biloba (Ginkgo biloba, Buyeo, Chungcheongnam-do, South Korea) of the genus Ginkgo in the Ginkgo family such as figure 1 , The xylem and phloem fibers can be observed by staining with phloroglycinol, and it can be confirmed that there is a cambium therebetween. To harvest the cambium-derived stem cells from such ginkgo biloba, the stem cells of the ginkgo biloba were first collected and immediately immersed in an antioxidant 100 mg / L ascorbic acid (L-ascorbic acid, DUCHEFA, The Netherlands) solution for delivery and storage. At this time, to harvest the same stem cells for utilizing the procambium, the residual branches were collected instead of the stems.

[0068]Thereafter, in 1% benomyl (benomyl, Dongbu Hannong Chemical, Korea), 1% chlorothalonil (daconil, Dongbu Hannong Chemical, Korea), 1% streptomycin sulphate (duchefa, The Net...

Embodiment 2

[0078] [Example 2: Proliferation and characteristic observation of stem cells derived from the cambium of the genus Ginkgo]

[0079] The cambium-derived stem cells isolated in Example 1 were placed in a bottle containing the following liquid medium and cultured in a shaker at 100 rpm at 25±1° C. under dark conditions. The cycle of subculture is fixed at 2 weeks, so that the cultured cells can always maintain high viability in the logarithmic growth phase.

[0080] Table 2: Suspension medium (medium 2)

[0081]

[0082] When observing the degree of cell aggregation (biological microscope C × 31, Olympus, Japan), the stem cells according to the present invention are as follows: Figure 4 As shown in A, it was confirmed that a large number of single cells were included in the suspension culture, and some of them existed as small-sized cell aggregates. ,Such as Figure 4 As shown in B, agglutination can be observed.

[0083] On the other hand, if Figure 5 As shown in A, ...

Embodiment 3

[0099] [Example 3: Treatment of elicitors and preparation of extracts from cambium-derived stem cells of Ginkgo biloba]

[0100][3-1: Handling of elicitors]

[0101] Stem cells cultured in suspension for 14 days as in Example 2 were collected and divided into two treatment groups to carry out the experiment. That is, (1) the 14-day suspension-cultured cell line (growth phase), (2) the 14-day suspension-cultured cell line was added to sterilized water with 3 to 5% by weight (g / L ) and methyl jasmonate 100 μM culture medium in the dark for 14 days (induction phase).

[0102] [3-2: Preparation of extract]

[0103] After the culture medium was removed from each of the two cell lines, 2 g of the freeze-dried freeze-dried cell line (Dry) was dissolved by stirring with 50 ml of 80% ethanol at 15° C. for 48 hours. After the dissolution, the extract powder obtained by centrifuging at 3,000 rpm for 20 minutes and freeze-drying the supernatant was dissolved in PBS, and the ethanol ext...

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PUM

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Abstract

The present invention relates to a stem cell derived from cambium of family Gingkoaceae and to method for the isolated culturing thereof. The stem cell derived from cambium of family Gingkoaceae according to the present invention is advantageous as it is isolated at a non-differentiated state without passing through a dedifferentiation process and stably maintained without causing a variation in the cellular growth rate and growth pattern even during a long-term culture, thereby enabling a mass culture. In addition, the stem cell derived from cambium of family Gingkoaceae according to the present invention showed an antioxidant effect similar to or higher than that of existing synthetic antioxidants when the radical-scavenging activity for scavenging radicals generated by the treatment of an oxidant is measured, and thus can be valuably used as an excellent anti-inflammatory composition.

Description

【Technical field】 [0001] The present invention relates to cambium-derived stem cells of the family Gingkoaceae and a method for isolating them. 【Background technique】 [0002] Plants used to mean food resources, but now they are sources of a wide spectrum of chemical substances including medicines, fragrances, pigments, pesticides, pigments, etc., and their meaning has been expanded. In particular, most of the useful substances derived from plants have physiological activities such as anti-virus, anti-bacteria, anti-cancer, and anti-oxidation ability, and are attracting attention as ideal resources that can be developed into new drugs. Research on the relationship between the chemical structure and activity of substances is actively being carried out. [0003] However, it is true that the development of physiologically active substances into pharmaceuticals is difficult, and the main reasons for this are as follows. First, the content of physiologically active substances i...

Claims

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Application Information

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IPC IPC(8): C12N5/04C12N5/02
CPCA01H4/005A01H7/00A61P39/06
Inventor 洪湳周陈荣雨李银庆洪宣美
Owner 深圳露泉实业有限公司
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