52results about "Gymnosperms" patented technology

The invention discloses polyketide synthase or a polyketide synthase mutant and application thereof, and belongs to the technical field of gene engineering. The polyketide synthase is AspiPKS4, AspiPKS5, AspiPKS6 and AspiPKS7 from cyathea spinulosa, FhPKS2, FhPKS3, FhPKS4, FhPKS5 and FhPKS6 from ficus himalayana, and CrPKS from floating fern. The mutant is obtained by carrying out single point mutation on the second amino acid of AspiPKS7 or deleting an N-terminal redundant sequence. The polyketide synthase or the mutant is used for replacing NnHisspS in an original FBP luminescence system, and the biological self-luminescence level can be remarkably improved. The invention further provides a simplified and improved FBP system which comprises the polyketide synthase or the polyketide synthase mutant, CPH, H3H and Luz, and the application convenience and the application luminescence capacity of the FBP system are improved.

The invention discloses a method for enhancing squalene synthesis through oil tea HMGR gene editing. According to the method, phosphorylation regulation and control sites Ser47 and / or Thr208 recognized by MAPK are targeted, and enzymatic activity inhibition is relieved and metabolic flux is enhanced by constructing phosphorylation resistance mutants (such as Ser-> Ala and Thr-> Val). The constructed editing vector can be introduced into camellia oleifera tissues in manners of PEG-mediated protoplast transient transfer, agrobacterium infection of calluses and the like, so that stable expression is realized. Experiments prove that the enzyme activity of HMGR in the editor is remarkably improved, the squalene accumulation amount is increased by 50% or above, the editing efficiency is high, expression is stable, and the method is suitable for high-value development of plant triterpenoid functional components and excellent strain breeding. The method also has the potential of popularization and application in other triterpenoid synthetic crops.

The invention belongs to the field of gene engineering, and relates to a plant genome directional editing tool based on transposon-coded nuclease. The invention aims to solve the technical problem that the application range of a plant genome editing tool based on CRISPR-Cas9 and Cas12a is limited to a great extent due to the fact that CRISPR-Cas9 and Cas12a are large in protein size and difficult to deliver. The invention provides transposon nuclease suitable for plant genome editing. The transposon nuclease is IsDge10, IsAam1 or enIscB subjected to codon preference optimization; and a gene editing system containing the three transposon nuclease is constructed. The gene editing system can be suitable for editing a coding region, a non-coding region and the like; the method can be widely applied to monocotyledonous plants, dicotyledonous plants, gymnosperm and the like.

A new and distinct cultivar of Thuja plant named ‘RutThu6’, characterized by its compact, rounding form and yellow foliage with orange tips; growth and heat tolerance in USDA Zone 8a, growth in full sun and afternoon shade conditions in landscape and full sun in container production, disease and insect resistance.

The application discloses a protein separated from Pinus tabulaeformis Carr. Pinus tabuliformis ​ and an encoding gene PtAMT2.8 of the protein, which can significantly enhance nitrogen absorption and transportation of plants. 31019b The PtAMT2.8 gene is introduced into a yeast mutant strain with a deficiency in ammonium nitrogen absorption function, so that the growth phenotype of the yeast mutant is recovered, and the PtAMT2.8 gene is introduced into Pinus tabulaeformis and Arabidopsis thaliana, so that the nitrogen absorption and transportation capacity of the transgenic plants can be significantly enhanced. The application has important significance for accelerating genetic improvement of plants and cultivating new varieties with high yield, high quality and multi-resistance.

The invention discloses a protein which is separated from pinus tabuliformis and is capable of remarkably enhancing nitrogen absorption and transport of a plant, and a coding gene PtAMT2.8 of the protein. The PtAMT2.8 gene is introduced into a yeast ammonium nitrogen absorption function deficient mutant strain 31019b, so that the growth phenotype of a yeast mutant can be recovered, and the nitrogen absorption and transport capability of a transgenic plant can also be remarkably enhanced by introducing the PtAMT2.8 gene into pinus tabulaeformis and a model plant arabidopsis thaliana. The invention has important significance in accelerating plant genetic improvement and cultivating high-yield, high-quality and multi-resistant new varieties.

The invention discloses a transcription factor TgWRKY65 of torreya grandis as well as a coding gene and application of the transcription factor TgWRKY65. The amino acid sequence of the transcription factor TgWRKY65 is shown as SEQ ID NO.2, and the nucleotide sequence of the coding gene is shown as SEQ ID NO.1. The invention further discloses a preparation method of the transcription factor TgWRKY65. According to the invention, a homologous expression material is constructed through cloning of the TgWRKY65 gene, a transgenic technology and gene silencing, and the application prospect in the aspect of increasing the content of the torreya grandis catechin is great; when the coding gene is over-expressed, the content of catechin is increased; when the coding gene is silenced, the content of catechin is reduced.

The invention belongs to the technical field of gene engineering, and particularly relates to a larch drought response gene LgLEA10, a protein and application thereof. The nucleotide sequence of the larch drought response gene LgLEA10 provided by the invention is as shown in SEQ ID NO.1, and the amino acid sequence of the protein expressed by the larch drought response gene LgLEA10 is as shown in SEQ ID NO.2. It is proved for the first time that the LgLEA10 gene of dahurian larch is a drought response regulation gene of dahurian larch. Tests prove that overexpression of the LgLEA10 gene can improve the drought resistance of larch by remarkably improving the activity of antioxidant enzyme and osmotic adjusting substances. The discovery provides important gene resources and theoretical basis for cultivating a new variety of larch with strong drought resistance.

A new cultivar of Chamaecyparis plant named ‘Edengra 23’ that is characterized by its upright, columnar and compact plant habit, its foliage that is thick and coarse in texture, and its very good growth in peat-free soil.

This invention relates to the field of plant genetic engineering technology, and in particular to a red pine. PkSHR Genes and their application in regulating somatic embryogenesis in Korean pine. The Korean pine mentioned... PkSHR The nucleotide sequence of the gene is shown in SEQ ID No. 1. The Korean pine provided by this invention... PkSHR Gene regulation of somatic embryogenesis in Korean pine, overexpression of Korean pine PkSHR The gene significantly improves the yield and quality of Korean pine embryos and promotes the growth of Korean pine seedlings.

The invention belongs to the technical field of plant biology, and particularly relates to a protein coded by a larch LarGRF1 gene and application of the protein to plant growth promotion. The invention aims to provide a new choice for plant genetic breeding by exploring growth regulatory factors (GRFs) of larch. The technical scheme of the invention is that the protein coded by the larch LarGRF1 gene has an amino acid sequence as shown in SEQ ID No. 2. Through overexpression of the MarGRF1 gene in dicotyledonous plants and monocotyledonous plants, it is found that an overexpression vector can promote proliferation of plant cells, especially proliferation of calluses and accelerate regeneration and growth.

The invention discloses a nucleotide sequence of a larch endogenous strong promoter Pro-LarGRP1A and an application of the larch endogenous strong promoter Pro-LarGRP1A. The invention relates to a method for creating and cultivating woody gymnosperm plants such as larch with precisely improved target agronomic characters such as biomass, disease resistance, insect resistance, stress resistance and the like through genetic transformation of a carrier expressed by a target gene driven by a Pro-LarGRP1A promoter sequence and a method for creating and cultivating the woody gymnosperm plants such as the larch with precisely improved target agronomic characters such as biomass, disease resistance, insect resistance, stress resistance and the like. The implementation effect shows that compared with the known conventional plant expression vector, the expression vector constructed by taking the nucleotide sequence of the Pro-LarGRP1A promoter region disclosed by the invention as the promoter element is used for transforming a larch receptor material through an agrobacterium-mediated genetic transformation method, a target gene in a positive transformed strain can be efficiently expressed, and the expression vector has the advantages that the expression vector is simple and convenient to construct, and the application prospect is wide. And the gene expression level is more than 8 times that of the conventional expression vector. The nucleotide sequence of the larch endogenous strong promoter Pro-LarGRP1A and the application of the larch endogenous strong promoter Pro-LarGRP1A disclosed by the invention have an important application value in efficient and low-cost cultivation of new germplasm, new materials and new varieties of woody gymnosperm plants such as high-biomass, high-volume, high-stress-resistance and high-quality larch.

This invention discloses a method for multi-gene aggregation breeding of larch and its application in cultivating new larch germplasm, lines, and varieties with excellent performance in terms of biomass, timber properties, and stress resistance. It includes the following steps: construction of a multi-gene aggregation expression backbone vector suitable for larch genetic transformation; cloning of genes regulating target traits such as biomass, timber properties, and stress resistance; construction of the multi-gene aggregation expression vector; larch genetic transformation, screening, and identification; and phenotypic observation and evaluation of the multi-gene-transferred larch materials. Results show that this method can introduce multiple target genes into larch recipient materials through a single genetic transformation, significantly improving the breeding efficiency of obtaining transgenic larch materials with excellent comprehensive traits. This method is of great value in overcoming the breeding bottlenecks of long larch growth cycles and a lack of precise techniques for synergistic improvement of multiple traits, and in the targeted and efficient cultivation of larch materials with excellent comprehensive traits.

This invention belongs to the field of genetic engineering technology, specifically relating to improving the drought resistance of larch trees. LgERF4 Genes and their applications. (The following is a separate section...) LgERF4 The nucleotide sequence of the gene is shown in SEQ ID NO.1. This invention is the first to experimentally demonstrate... LgERF4 Gene overexpression can enhance the drought resistance of larch by significantly increasing the activity of antioxidant enzymes and the content of osmotic regulators. This discovery provides important genetic resources and theoretical basis for breeding new larch varieties with strong drought resistance.

The present invention relates to the technical field of synthetic biology. Provided in the present invention are a biological enzyme that catalyzes the formation of a three-membered or four-membered oxygen-containing heterocyclic structure of a taxane molecule, a gene, a biological material, and the use of the biological enzyme, gene and biological material. The biological enzyme comprises: at least one of amino acid sequences as shown in SEQ ID NOs: 25-48; a fusion protein thereof; a mutant protein thereof; and a homologous protein thereof. The provided biological enzyme can catalyze the formation of a three-membered or four-membered oxygen-containing heterocyclic structure of a taxane molecule, thereby providing new gene resources in terms of increasing the yield of related compounds having an oxetane ring group and an oxirane ring group such as paclitaxel and completing the synthetic pathway thereof; and the nucleotide sequence can be used for modifying a chassis host by means of genetic engineering and metabolic engineering strategies, and producing drugs such as paclitaxel and intermediates thereof, and has significant economic and social values.

The application discloses a method for promoting adventitious root development of plants and a special ThSUC2 gene and expression protein thereof, and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the gene is shown as SEQ ID NO. 1, and the amino acid sequence of the expression protein is shown as SEQ ID NO. 2. In the experiment, the ThSUC2 gene is transferred into a poplar, the expression amount of the gene in the transgenic plant is increased by 200-600 times, a large amount of adventitious roots appear on the stem under waterlogging stress, and the ADH enzyme activity and the ACC content are significantly enhanced. The results show that the ThSUC2 gene is a key factor for regulating the adventitious root development and waterlogging resistance of Taxododendron and has important application value in the stress resistance molecular breeding of Taxododendron species.

The invention belongs to the technical field of gene engineering, and particularly relates to a salt-tolerant gene ThGPX4 and application thereof. The invention provides a salt-tolerant gene ThGPX4. The salt-tolerant gene ThGPX4 is used for coding an amino acid sequence as shown in SEQ ID NO: 2. The invention finds that the ThGPX4 gene can significantly improve the salt tolerance of arabidopsis thaliana after overexpression in arabidopsis thaliana, the salt-tolerant gene ThGPX4 is constructed into a plant expression vector, genetic transformation is performed through an agrobacterium tumefaciens-mediated genetic transformation method, and a new germplasm with a salt-tolerant characteristic can be effectively obtained. The method is of great significance to cultivation of new varieties of excellent crops and wide application in production.

Root-specific expression of carbonic anhydrase increases the plant's ability to sequester environmental carbon. Herein, plants are genetically engineered so that the plant's heritable genetic material contains a gene encoding a transportable carbonic anhydrase protein under the control of a promoter active in root cells, such that carbonic anhydrase is transported from the plant's roots into the extracellular environment. These genetically modified plants demonstrate increased levels of carbonic anhydrase activity in the extracellular environment of plant roots, which enhances carbon sequestration in the extracellular environment, such as soil.

The invention belongs to the field of gene engineering, and relates to a gramineous plant genome directional editing tool based on transposon-coded nuclease. The invention aims to solve the technical problem that the application range of a plant genome editing tool based on CRISPR-Cas9 and Cas12a is limited to a great extent due to the fact that CRISPR-Cas9 and Cas12a are large in protein size and difficult to deliver. The invention provides transposon nuclease suitable for genome editing of gramineous plants. The transposon nuclease is IsDge10, IsAam1 or enIscB subjected to codon preference optimization; and a gene editing system containing the three transposon nuclease is constructed. The gene editing system can be suitable for editing a coding region, a non-coding region and the like; the method can be widely applied to gramineous plants.

The invention relates to the technical field of plant genetic engineering, in particular to a pinus koraiensis PkDof5.6 gene and application of the pinus koraiensis PkDof5.6 gene to regulation and control of pinus koraiensis somatic embryogenesis. The nucleotide sequence of the PkDof5.6 gene of the Korean pine is as shown in SEQ ID No. 1 (Sequence Identifier Number 1). The Korean pine PkDof5.6 gene provided by the invention regulates Korean pine somatic embryogenesis, and overexpression of the Korean pine PkDof5.6 gene significantly improves the yield and quality of the Korean pine somatic embryogenesis, and promotes the growth of Korean pine somatic embryogenesis seedlings. The method has important significance in optimizing a Korean pine somatic embryogenesis system and realizing efficient large-scale propagation.

A new cultivar of Thuja plant named ‘GK01’ that is characterized by its strongly fastigiate and compact plant habit and its dark green foliage.

The invention discloses a gingko GbF3H gene as well as an expression protein and application thereof, and belongs to the field of plant molecular biology. The GbF3H gene is obtained by analyzing and screening on the basis of ginkgo transcriptome data in the earlier stage of a research group, the GbF3H gene is flavanone 3-hydroxylase, the nucleotide sequence of the GbF3H gene is shown as SEQ ID NO.1, and the amino acid sequence of expression protein of the GbF3H gene is shown as SEQ ID NO.2. A GbF3H overexpression vector is constructed and the nicotiana benthamiana is transformed, so that the synthesis of flavonoid substances in a transgenic plant is obviously changed, the difference of 28 flavonoid metabolites is obvious, and the contents of 9 flavonoid substances such as formononin are up-regulated. The invention provides a theoretical basis, a valuable gene resource and a method for analyzing a ginkgo flavone synthesis mechanism and improving the flavone yield through metabolic engineering, and has important significance on development and utilization of ginkgo resources and cultivation of new germplasm with medicinal value.

The invention discloses a gingko GbHCT1 gene as well as an expression protein and application thereof, and belongs to the field of plant molecular biology. The GbHCT1 gene is obtained by analyzing and screening based on data of a ginkgo transcriptome in the earlier stage of a research group, the nucleotide sequence of the GbHCT1 gene is as shown in SEQ ID NO.1, and the amino acid sequence of expression protein of the GbHCT1 gene is as shown in SEQ ID NO.2. A GbHCT1 overexpression vector is constructed and the nicotiana benthamiana is transformed, so that the content of ten flavonoid substances such as luteolin and isorhamnetin-3-O-glucoside in the transgenic GbHCT1 tobacco is remarkably increased. KEGG enrichment shows that differential metabolites are mainly enriched in a flavone synthesis pathway. The application reveals that the ginkgo GbHCT1 gene has dual effects on synthesis of flavonoid compounds by regulating carbon source distribution of a phenylpropane metabolic pathway, and a theoretical basis, a gene resource and a method are provided for secondary metabolism regulation and genetic improvement of ginkgo.

Described herein are cultured gum base ingredients and methods of producing thereof by plant cell culture, also known as plant cell fermentation. The cultured gum base ingredients described herein may comprise one or more terpenoid compounds, such as triterpenoid acetates and polyisoprene. The method of producing the gum base ingredients comprises culturing in a culture medium of a plurality of plant cells derived from a plant that produces a gum base ingredient.

The invention discloses application of a Japanese larch CHS1 gene or an encoding protein thereof in improving pine wood nematode resistance of pine trees. The invention finds that the resistance of embryogenic calluses to pine wood nematodes can be remarkably improved by performing overexpression on the Japanese larch CHS1 gene in the Japanese larch embryogenic calluses, so that the invention provides the application of the Japanese larch CHS1 gene or the encoded protein thereof in improving the resistance of pine trees to the pine wood nematodes, and the application comprises the following steps: overexpressing the CHS1 gene in the pine trees, the expression level of the CHS1 gene is enhanced or the function or activity of the encoded protein is enhanced. The method has important economic value and ecological value in the aspects of improving the pine wood nematode resistance of the pine trees and the like.

The present application relates to the technical field of plant genetic engineering, and particularly relates to application of LkHY5 gene in regulation of somatic embryogenesis of hybrid larch. The present application shows that overexpression of LkHY5 gene promotes somatic embryogenesis on a culture medium containing 30 mg / L of exogenous hormone ABA. Overexpression of LkHY5 gene significantly increases expression levels of LkGBSS1-3 and LkTAN1-1. Overexpression of LkHY5 gene significantly increases contents of starch, maltose, glucose and soluble sugar in callus. Overexpression of LkHY5 gene significantly increases microtubule stability in callus.

The invention discloses a transcription factor TgHSFA2e1 of torreya grandis as well as a coding gene and application of the transcription factor TgHSFA2e1. The amino acid sequence of the transcription factor TgHSFA2e1 is shown as SEQ ID NO.2, and the nucleotide sequence of the coding gene is shown as SEQ ID NO.1. The invention further discloses a preparation method of the transcription factor TgHSFA2e1. By cloning the TgHSFA2e1 gene, a heterologous (tobacco) and homologous (torreya grandis) overexpression material of the TgHSFA2e1 is constructed, and the TgHSFA2e1 gene has a relatively great application prospect in the aspect of improving the content of the catechin in the torreya grandis; when the coding gene is over-expressed, the content of catechin is increased.

The invention discloses a creation method of low-toxicity taxus chinensis, an extract of the low-toxicity taxus chinensis, a method and application of the low-toxicity taxus chinensis. The method comprises the following steps: firstly, knocking out a T14H gene of 'Kehong No.1' taxus chinensis of which the variety right has been applied through a CRISPR / Cas9 gene editing technology, and creating a low-toxicity new germplasm of which the paclitaxel content is reduced by more than 95% from the source; then taking the taxus chinensis as a raw material, sequentially performing supercritical CO2 degreasing and NADES aqueous two-phase system directional extraction, integrating real-time monitoring and feedback regulation of a PAT intelligent control system, and finally performing membrane separation and refining to obtain the taxus chinensis extract with ultrahigh safety and high purity. According to the invention, a full-chain scheme from breeding to products is formed, and the obtained products have wide application prospects in the fields of medicines, cosmetics and health-care products / functional foods.

The invention relates to the technical field of plant genetic engineering, in particular to application of an LkHY5 gene in regulation and control of hybrid larch somatic embryogenesis. Results of the invention show that overexpression of the LkHY5 gene promotes somatic embryogenesis on a culture medium containing 30 mg / L of exogenous hormone ABA. The expression levels of LkGBSS1-3 and LkTAN1-1 are remarkably improved by overexpression of the LkHY5 gene. The overexpression of the LkHY5 gene significantly improves the contents of starch, maltose, glucose and soluble sugar in the callus. The overexpression of the LkHY5 gene significantly improves the stability of the callus microtubules.

A method for increasing plant responses to pests and / or pathogens by increasing the production of one or more plant defense compounds derived from anthranilates in plants. Also provided is a method for identifying and selecting microbial strains that can be used in plant treatments to increase plant responses to attacks by pests and / or pathogens.