282 results about "Fungal endophyte" patented technology

The invention provides methods for preparing a conifer seedling with increased tolerance to a pest. A conifer seedling is inoculated with an isolated endophyte when the conifer seedling is susceptible to colonization by the endophyte.

The present invention relates to the discovery of a novel endophytic fungus, Muscodor vitigenus, from the liana, Paullinia paullinioides. This fungus produces naphthalene under certain cultural conditions that has chromatographic and mass spectral properties that are identical to authentic naphthalene. In a preferred embodiment, the naphthalene in the gas phase of M. vitigenus is useful in the repellency of unwanted insect pests. The unique biological activity of the novel endophyte suggests a wide range of potential practical applications, particularly in the area of insect repellents, insecticides, antimicrobials, anthelmintics and vermicides.

The disclosure provides materials and methods for conferring improved plant traits or benefits on plants. The materials can include a formulation comprising an exogenous endophytic bacterial population, which can be disposed on an exterior surface of a seed or seedling, typically in an amount effective to colonize the plant. The formulations can include at least one member selected from the group consisting of an agriculturally compatible carrier, a tackifier, a microbial stabilizer, a fungicide, an antibacterial agent, an herbicide, a nematicide, an insecticide, a plant growth regulator, a rodenticide, and a nutrient.

This invention relates to methods and materials for modulating seed nutritional quality traits of seeds produced by a wheat, cotton, soybean, or maize plant, said plant having been heterologously disposed to, or grown from, a plant element treated with an endophyte.

The invention provides an isolated culture of a Neotyphodium endophyte of an Elymus canadensis host plant, wherein the endophyte reproduces asexually and enhances the agronomic characteristics of the host plant. Methods for inoculating the host plant with the endophyte, for propagating the host-endophyte combination, and for detecting the presence of the endophyte and of its metabolites within a host plant are also described.

Described herein are compositions and methods related to isolated Trichoderma harzianum and strains thereof.

The invention discloses an endophyte which is classified and named Bacillus megaterium and is completely named Bacillus megaterium SAN1. The endophyte is preserved in the China General Microbiological Culture Collection Center (CGMCC) on March. 11, 2013 and has a preservation number of CGMCC No.7295. The invention also discloses an application of the endophyte in repair of facility soil secondary salinization and Cd combined pollution. The Bacillus megaterium CGMCC 7295 disclosed by the invention can be used for prompting growth of plants, improving physical and chemical properties of soil, promoting the plants to absorb Cd, effectively eliminating Cd pollution in the soil, efficiently converting nitrate nitrogen of soil and alleviating the hazards of secondary salinization; and due to combined action between the Bacillus megaterium CGMCC 7295 and the plants, the facility soil secondary salinization and the heavy metal Cd combined pollution can be repaired.

The invention discloses a burkholderia pyrrocinia JK-SH007 which is classified and named as burkholderia pyrrocinia and preserved in China center for type culture collection (CCTCC) with the preservation number of M 209028 and the preservation date of February 18th, 2009. The invention also discloses the application of the burkholderia pyrrocinia in controlling dothiorella gregaria and promoting poplar to grow. The invention further provides a bactericide for controlling dothiorella gregaria, which is obtained by inoculating the JK-SH007 in a culture medium to be fermented and cultured. The JK-SH007 is plant endophyte which has strong inhibiting effect for botryosphaeria dothidea of the poplar and antagonism for various pathogenic fungis, and has the advantages of simple culture condition, easy storage and commercial process as well as good developing and applying prospect.

The invention relates to the technical field of microbes, and discloses a separation method for plant endophyte. The separation method comprises the following steps: (1) pretreating plant tissue; (2) culturing plant tissue; and (3) separating endophyte. The disclosed separation method for plant endophyte is capable of effectively removing surface bacteria, fungi, actinomyces and other microbes, and avoiding endophyte separation effect caused by mixing of mixed bacteria. Endophyte in the plant tissue recovers growth after tissue culture, and disinfection processing does not need performing in operation of separating endophyte, so that the damage effect of a disinfectant on plant tissue and endophyte is substantially reduced, the survival probability of endophyte is increase, and the number of endophyte obtained through separation is obviously improved.

A grass with improved environmental stress tolerance and low animal toxicity is disclosed where the grass is infected with an isolated endophyte selected from the groups consisting of USDA-PI no. 269850, Ijoukak, Morocco 92060, Boumalne-de-Dades, Morocco KBG 5921, USDA-PI no. 516560, Oujda, Morocco KBG 5892, and mixtures and combinations thereof. A method for stably infecting the grasses is also disclosed.

A process and method for the production of endophytes as plant inoculant products, specifically Clonostachys rosea strain 88-710, for the promotion of plant vigor, health, growth and yield are disclosed. The endophyte, Clonostachys rosea strain 88-710 produces a fungal conidial preparation by utilizing a discrete solid substrate fermentation system, namely Potato Dextrose Agar or Malt Extract Agar. Additionally, the endophyte, Clonostachys rosea strain 88-710, can act as an inoculant to stimulate and have an additive effect with rhizobium bacteria on the production of nitrogen fixing nodules on legumes and growth enhancement e.g. beans, soybeans, peas and alfalfa. As well, Clonostachys rosea strain 88-710, can combine with rooting hormones, e.g. indole-3-butyric acid (IBA) to provide inoculant and rooting benefits to cuttings / transplants of plants.

The invention discloses a separation and selection method of antibiotic antitubercular endophyte, which comprises the steps as follows, the preparation of antibiotic antitubercular plant tissue, the surface sterilization and the sterility test, the separation and the purification of the endophyte, the ferment of endogenetic fungi, the ferment of endogenetic bacterium, the process of the zymotic fluid, the process of the thalli, the selection of the effective antibiotic bacterial strains, the selection of the effective antitubercular bacterial strains and the preservation of the strains. The separation and selection method of antibiotic antitubercular endophyte ensures the realization of obtaining the effective antibiotic antitubercular matter from microorganisms, thanks to separation and selection method, a batch of effective bacterial strains are obtained.

The invention discloses a method for tissue culture method of huperizia serrata. The method comprises the following steps of: selecting an explant; killing surface bacteria; performing inoculation and cultivation; killing endophyte; performing propagation cultivation; and performing rooting cultivation. The huperizia serrata is a precious medicinal pteridophyte which has a tough requirement on a habitat, grows slowly under a natural condition and has a long production period. In a conventional method, propagation coefficient and biomass are low and requirements on medicament production cannot be met. Due to the adoption of the method, the differentiation rate of a huperizia serrata test tube plantlet is up to 90 percent, monthly multiplication multiple is up to 2.1 to 3.5, rooting rate is up to 100 percent, a root system grows quickly, root number is up to 1.6 to 3.4 per plant, a regeneration seedling grows healthily and the root system is developed. The method has the advantages of effectively restraining the pollution and browning of a huperizia serrata explant, promoting the survival and growth of the explant and providing important technical support for the realization of manual mass planting of the huperizia serrata along with simpleness, convenience, practicability, high efficiency and low cost.

The invention discloses an extraction and purification method of total plant endophyte genome DNA for colony analysis. The method comprises the following steps of: shearing and grinding the surface bacteria removed fresh plant tissues into paste, soaking the plant tissues into sterilized phosphate buffer solution, performing thermostatic shaking for 1 hour in a table concentrator to separate out microbial cells from the plant tissues, standing, transferring the suspension to a centrifuge tube, performing centrifugal collection on microbial bacteria, then adding lysing solution, shaking and uniformly mixing the solution to release DNA from the cells, repeatedly extracting protein by chloroform-isoamylol, performing centrifugal sedimentation with isopropanol and washing with 70 percent ice ethanol, performing purification by using a DNA specific centrifugal adsorption column to obtain total crude extracted genome DNA, and finally, performing polymerase chain reaction (PCR) amplificationby using special primers to obtain high-quality plant endophyte specific DNA fragments for subsequent analysis. The invention has the advantages that: the method is simple and effective, high in quality, low in pollution and the like, and provides high-quality guarantee for comprehensively and completely researching a plant endophyte colony structure.

The invention discloses a bacillus pumilus JK-SX001 which is classified and named bacillus pumilus and collected in a China center for type culture collection (CCTCC) and has the collection number ofM 209026 on February 18th, 2009. The invention also discloses applications of the bacillus pumilus in preventing and treating dothiorella gregaria and promoting the growth of a poplar. The invention also provides a bactericide for preventing and treating the dothiorella gregaria, which is obtained by vaccinating the JK-SX001 in a culture medium to ferment and culture. The JK-SX001 is a plant endophyte, has obvious inhibiting effect on dothiorella gregaria, simple culture condition, easy storage and favorable development and application prospect and is easy for industrialized production.

The invention discloses a sphingomonas paucimobilis strain and an application thereof. The sphingomonas paucimobilis strain is named as sphingomonas paucimobilis QHZJUJW, with the preservation number: CGMCC No.7783. The stain is endophyte derived and screened from seeds of wild microporous grass in Qinghai-Tibet Plateau, can be used for industrialized production of gellan gum and provides a new way for the industrialized production of the endophyte and gellan gum.

The present invention relates to isolated strains of a Streptomyces spp. which are endophytes of dicotyledonous plants and to methods for selecting such strains. The present invention also relates to a biologically active compound called coronamycin obtained from endophytic Streptomycetes isolated from higher plants. The present invention further relates to compositions of such compounds and to methods of protecting plants against attack by a plant pathogen and methods of inhibiting bacterial growth, fungal growth, viral infection, growth of parasitic organisms, and cancer cell growth with such compositions.

The invention discloses a sedum alfredii endophyte and an application thereof. The endophyte is named as Acinetobacter calcoaceticus Sasm3 with a preservation number of CGMCC NO.7334. The invention also discloses an application of the endophyte in plant-microorganism combined remediation on heavy metal contaminated soil. The application comprises the steps of: inoculating sedum alfredii with the endophyte and planting the sedum alfredii inoculated with the endophyte in the heavy metal contaminated soil to be remedied; and from the second growing season of the sedum alfredii inoculated with the endophyte, re-inoculating the sedum alfredii with the endophyte in each growing season, and harvesting the sedum alfredii. The endophyte can be used for enhancing the resistance of the sedum alfredii to high-concentration heavy metal so as to promote the healthy growth of the sedum alfredii in the environment of high-concentration heavy metal, thereby increasing the biomass of the sedum alfredii and enhancing the remediation efficiency of the sedum alfredii to the heavy metal contaminated soil.

The present invention relates to compositions containing the fungus Alternaria alternata and, or the secondary metabolites thereof, belonging to the family of the diketopiperazines (DKPs), for controlling the pathogen fungus Plasmopara viticola.

The invention relates to two ralstonia-solanacearum-resistant endophytic bacillus velezensis strains and application thereof. By separation, screening and purification in roots of healthy tobacco plants in a ralstonia solanacearum occurrence area of the Enshi Prefecture, two biocontrol bacillus endophyticus strains R-3 and R-9 are obtained, have evident resistance effects on ralstonia solanacearumand are both capable of generating high-temperature-resistant ralstonia-solanacearum-resistant substances. By gyrB gene sequence identification and phylogenetic tree construction, results show that the R-3 and R-9 are both bacillus velezensis; by potted plant root irrigation experimental verification, the bacilli R-3 and R-9 have great colonization performance in tobacco roots and can enter stemsthrough root transmission; by field experimental verification, bacillus velezensis R-3 and R-9 are effective in ralstonia solanacearum prevention and treatment, remarkably superior to chemical agentkasugamycin and free of pollution. The biocontrol endophyte research field is further enriched by researching on the bacilli R-3 and R-9, and a guiding significance to ralstonia solanacearum prevention and treatment is achieved.

The invention provides bacillus subtilis BS193 for preventing and controlling a potato late blight and application of bacillus subtilis BS193 and belongs to the technical field of crop disease prevention and control. A strain is Bacillus subtilis BS193 and has been recorded and preserved by China General Microbiological Culture Collection Center at March 27, 2017 with a preservation number being CGMCC No. 13936. The strain inhibits growth of germs of the potato late blight by generating active substances and expresses better preventing and controlling effect to the potato late blight. The fungicide can be used for atomizing or root irrigation of a potato plant when being applied and can also be used by being mixed with an organic fertilizer to prepare a bacterial fertilizer. The bacillus subtilis BS193 provided by the invention is obtained by being extracted from a tomato plant body, belongs to endophyte, is nontoxic, does not have pathogenicity, and thus having a good application prospect in biological prevention and control of the potato late blight.

The invention provides a method for obtaining plant endophytes by separation, and the method comprises the steps of plant pre-treatment, surface disinfection, leaching of the endophytes, separation and purification of the endophytes, and the like; compared with the ordinary method, the method not only utilizes mechanical grinding and shearing force to leach the plant endophytes, but also adds a surfactant and a cellulose enzyme to decompose plant fibers and plant cell walls, thereby leading the endophytes in plant tissues to be released more fully and obtaining better separation effect of the plant endophytes.

The invention relates to microbe technique field, endophytic fungus of Chinese Holly of the invention collected from Chinese Holly living plant of Tianmu Mountain of Linan City is obtained by endophytic fungus microbe separation technology, named as Trichoderma harzianum Rifai stem L identified by microbial taxonomy stored by general microbilogy centre of China Forestry Culture Collection Center on date of august 14th 2006, registration number is CGMCC NO.1780. The most prominent feature is the stem can generate activated component seaquiterpenoids Trichothec-9-en-4-ol,12,13-epoxy-,acetate,(4beta)-(8CI,9CI) by fermentation for inhibiting phytopathogenic fungi bacteria, it is an important microbe resource in agriculture.

The present invention relates to new methods of selecting and breeding organisms, in particular organisms which exhibit symbiotic behaviour with symbionts, and to new organisms and symbiota developed thereby, such as plant or grass and endophyte symbiota. Multiple symbionts are deployed in multiple organisms and selected for improved symbiotic compatibility and performance early in the breeding process. Methods include producing improved organisms from germplasm, by inoculating host organism germplasm libraries with symbionts selected from symbiont libraries and selecting improved host organisms exhibiting desired symbiota characteristics, and selection of organism-symbiont associations with a desired genetic and metabolic profile by metagenomic analysis of nucleic acid libraries from an organism or organism-symbiont association.

To provide a grass having improved characteristics, an endophyte having endophytic fungi which produce an insect-resistant alkaloid is artificially introduced into a grass by inoculation so that the endophyte lives in and infects the grass.

The invention discloses a paenibacillus polymyxa bacterial strain and an application thereof. The paenibacillus polymyxa bacterial strain is PBSZ123, the preservation name is paenibacillus polymyxa (paenibacillus polymyxa), the paenibacillus polymyxa bacterial strain is preserved in China General Microbiological Culture Collection Center, CGMCC, the preservation work address is No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing, the preservation date is August 14, 2019, and the preservation No. is CGMCC No.18386. The paenibacillus polymyxa is obtained by separation from parispolyphyllavar.yunnanensis (Franch.)Hand endophyte, and has favorable inhibition effects on main pathogenic bacteria of parispolyphyllavar.yunnanensis (Franch.)Hand endophyte diseases of botrytis cinerea, phoma sp. and pestalotiopsis sp.

The invention discloses plant endophyte for efficiently degrading phthalic acid ester and an application of the plant endophyte. The plant endophyte is a Bacillus subtilis Enb-N strain, the strain is collected in a Guangdong Microbiological Culture Collection Center on May 4, 2017, and the collection number of the strain is GDMCC NO: 60173. The strain can use phthalate as a sole carbon source and energy for growth and propagation, the degrading rate of DBP (dibutyl phthalate) can reach 95% or more, the strain can be used for treating phthalate pollutants, further has the functions of secreting indoleacetic acid and degrading potassium and phosphorus and has a certain resistance for common antibiotics, growth of the strain has strong resistance to Cd and Hg ions, and the strain has strong environmental adaptability and large application prospect.

Clonostachys rosea strains have novel usefulness as inoculants of plants promoting plant vigor, health, growth, yield and a reduction of competitive stress caused by other fungi when used alone or sequentially with many fungicides in an integrated pest management system (IPM). Seed and foliar uses are shown to inoculate and subsequently achieve endophytic colonization of the portion of the plant treated. While the germinating conidia of this organism has been shown to tolerate several fungicide groups, the established mycelium of Clonostachys rosea is significantly more tolerant to systemic fungicides facilitating use in seed and foliar applications. Seed treatment and colonization may occur at any time after harvest resulting in endophyte enhanced seed that ignores or is marginally altered by other fungal organisms and may be suitable for Feed, Food and Seed uses.

The invention concerns a method for the phytoremediation treatment of a medium contaminated with at least one element selected from the group consisting of (preferably water soluble and volatile) organic pollutants, heavy metals, radionuclides or a mixture thereof, comprising the step of cultivating upon the contaminated medium a plant associated with an endophytic microorganism able to improve the phytoremediation of the plant, to reduce phytotoxicity of chemicals. The invention further relates to methods for improving phytoremediation by directly modifying members of the endogenous endophytic community of a plant, via horizontal gene transfer. Another part of the invention relates to plants associated with such endophytes and / or plants with a modified endogenous endophytic community.