190 results about "Bacterial population" patented technology

The invention relates to methods, uses, systems, arrays, engineered nucleotide sequences and vectors for inhibiting bacterial population growth or for altering the relative ratio of sub-populations of first and second bacteria in a mixed population of bacteria. The invention is particularly useful, for example, for treatment of microbes such as for environmental, medical, food and beverage use. The invention relates inter alia to methods of controlling microbiologically influenced corrosion (MIC) or biofouling of a substrate or fluid in an industrial or domestic system.

The invention relates to the technical field of organic fertilizer production, in particular to a bio-organic fertilizer production process data system and an extraction method. The method comprises the following steps: acquiring a raw material information set and a process parameter set; calculating a reference flora rate based on microbial activity characteristics of the raw material information set; marking a time sequence timestamp for the raw material information set and the process parameter set, and constructing a fermentation behavior track; analyzing the microbial activity characteristics of the fermentation behavior track, and calculating the flora reaction speed; therefore, by constructing a multi-source heterogeneous data-driven fermentation behavior track and quality mapping system, the problems of data isolation, state recognition lag and process deviation and incapability of accurate positioning in traditional bio-organic fertilizer production are solved, and the intelligent diagnosis capability and quality controllability of the production process are improved.

The invention relates to methods, uses, systems, arrays, engineered nucleotide sequences and vectors for inhibiting bacterial population growth or for altering the relative ratio of sub-populations of first and second bacteria in a mixed population of bacteria. The invention is particularly useful, for example, for treatment of microbes such as for environmental, medical, food and beverage use. The invention relates inter alia to methods of controlling microbiologically influenced corrosion (MIC) or biofouling of a substrate or fluid in an industrial or domestic system.

The invention relates to methods, uses, systems, arrays, engineered nucleotide sequences and vectors for inhibiting bacterial population growth or for altering the relative ratio of sub-populations of first and second bacteria in a mixed population of bacteria. The invention is particularly useful, for example, for treatment of microbes such as for environmental, medical, food and beverage use. The invention relates inter alia to methods of controlling microbiologically influenced corrosion (MIC) or biofouling of a substrate or fluid in an industrial or domestic system.

The invention relates to the technical field of biological process intelligent control, and discloses a pomegranate leaf fermentation process optimization and intelligent control system based on digital twinning. According to the system, the Internet of Things and a visual sensor are integrated through a multi-source data sensing module, so that synchronous acquisition and fusion of temperature, pH, dissolved oxygen, flora density and image data are realized; the intelligent data preprocessing module extracts high-quality features by adopting a self-adaptive filtering technology; the digital twin simulation engine module drives the virtual model to dynamically update based on real-time data; the deep prediction module accurately predicts the fermentation trend through a deep learning algorithm; the intelligent diagnosis module realizes abnormal root cause analysis by using a knowledge graph; the reinforcement learning optimization module autonomously generates an optimal control strategy based on the diagnosis result; and the self-adaptive execution module accurately regulates and controls fermentation parameters. According to the system, multi-dimensional perception, high-precision prediction and adaptive optimization control of the fermentation process are realized, and the accuracy and stability of process control are effectively improved.

Described are methods and antibodies useful for reducing, eliminating, or preventing infection with a bacterial population in an animal. Also described herein are antigens useful for targeting by heavy chain antibodies and VHH fragments for reducing a bacterial population in an animal.

The invention relates to methods, uses, systems, arrays, engineered nucleotide sequences and vectors for inhibiting bacterial population growth or for altering the relative ratio of sub-populations of first and second bacteria in a mixed population of bacteria. The invention is particularly useful, for example, for treatment of microbes such as for environmental, medical, food and beverage use. The invention relates inter alia to methods of controlling microbiologically influenced corrosion (MIC) or biofouling of a substrate or fluid in an industrial or domestic system.

The present invention provides a method for treating heap leaching cyanide tailings of gold mines, a composite bacterial community, a bacterial agent and a preparation method. The composite bacterial community comprises a TDB-1 strain, a TCD-1 strain and a Z-90 strain, wherein the TDB-1 strain is a Pseudomonas putida strain, and the deposit number is CGMCC No.21602. The TCD-1 strain is a Methylorubrum pseudosasae, and the deposit number is CGMCC NO.24728. The Z-90 strain is a Burkholderia sp., and the deposit number is CGMCC No.9970. The above-mentioned composite bacterial community can effectively remove cyanide, thiocyanide and metal-cyanide complexes. The present method has the advantages of simple operation, high reduction efficiency, stable effect, good impact resistance and low cost.

Provided are compositions and methods for selectively reducing the amount of antibiotic resistant and / or virulent bacteria in a mixed bacteria population, or for reducing any other type of unwanted bacteria in a mixed bacteria population. The compositions and methods involve targeting bacteria that are differentiated from other members of the population by at least one unique clustered regularly interspaced short palindromic repeats (CRISPR) targeted DNA sequence. The compositions and methods can be readily adapted to target any bacteria or any bacteria plasmid, or both.

The invention belongs to the technical field of pericarpium citri reticulatae fermentation, and relates to a fermentation temperature control method and system.The fermentation temperature control method comprises the steps that antifouling treatment data of a material is obtained, the water content of a mucus layer in the material is detected, and the total amount of biological metabolism heat production is calculated in combination with the self-weight pressure of the material; the temperature distribution of the fermentation heap is adjusted through coupling control in the three-stage heating and dynamic maintaining stage; the surface flora is guided to migrate to a high magnetic field area at the middle upper part, and the aggregation concentration of the bottom heat-producing flora is inhibited; when it is detected that the water content of the middle layer exceeds a threshold value, the directional water absorption program of the moisture conducting film is activated, and aromatic substances in overflowing liquid are separated and recycled; releasing aroma capture particles according to environment humidity sudden change data, and synchronously opening a directional heat dissipation air channel to balance the temperature difference of a material layer; and analyzing the actual yield of the target component in the finished product, and optimizing the initial control parameters of the next batch based on the deviation ratio with the theoretical value. The method solves the problem that the dynamic heat production rule of the flora growth stage cannot be adapted in real time, and excessive temperature rise or invalid heat dissipation is caused.

The present invention provides a composite bacterial community for degrading polycyclic aromatic hydrocarbons and its application, belonging to the field of microbial technology. The composite bacterial community of the present invention comprises Fusarium solani E1 and Rhodococcus erythroxysporum E2; the preservation number of Fusarium solani E1 is CGMCC No: 41063, and the preservation number of Rhodococcus erythroxysporum E2 is CGMCC No: 29266. The present invention combines fungi and bacteria to synergistically degrade PAHs in the repair of PAHs. The composite bacterial community has better PAHs degradation effect and composite bacterial growth status than single bacteria, and has good effects on the degradation of high- and low-ring PAHs.

The present disclosure relates generally to conjugated plasmids comprising a multiple entry exclusion system derived from a plasmid carrying a gene conferring undesired traits of interest, such as pathogenic traits, such as antimicrobial drug resistance (AMR), virulence determinants, metal resistance, or other undesired traits, such as, for example, multiple entry exclusion systems derived from plasmids carrying a gene conferring undesired traits of interest. And the use of those probiotic plasmids for protecting bacterial populations (e.g., present in the gut, present in the environment, etc.) from obtaining plasmids imparting the undesirable traits and thereby preventing the propagation of the undesirable traits (e.g., AMR, virulence, metal resistance, etc.) within microbiota.

Provided are compositions and methods for selectively reducing the amount of antibiotic resistant and / or virulent bacteria in a mixed bacteria population, or for reducing any other type of unwanted bacteria in a mixed bacteria population. The compositions and methods involve targeting bacteria that are differentiated from other members of the population by at least one unique clustered regularly interspaced short palindromic repeats (CRISPR) targeted DNA sequence. The compositions and methods can be readily adapted to target any bacteria or any bacteria plasmid, or both.

Provided are compositions and methods for selectively reducing the amount of antibiotic resistant and / or virulent bacteria in a mixed bacteria population, or for reducing any other type of unwanted bacteria in a mixed bacteria population. The compositions and methods involve targeting bacteria that are differentiated from other members of the population by at least one unique clustered regularly interspaced short palindromic repeats (CRISPR) targeted DNA sequence. The compositions and methods can be readily adapted to target any bacteria or any bacteria plasmid, or both.

Provided are compositions and methods for selectively reducing the amount of antibiotic resistant and / or virulent bacteria in a mixed bacteria population, or for reducing any other type of unwanted bacteria in a mixed bacteria population. The compositions and methods involve targeting bacteria that are differentiated from other members of the population by at least one unique clustered regularly interspaced short palindromic repeats (CRISPR) targeted DNA sequence. The compositions and methods can be readily adapted to target any bacteria or any bacteria plasmid, or both.

The invention relates to a device (10) for biomethanation of a CO- and / or H2-containing gas (8), wherein the biomethanation device is characterized in that it comprises:a first reactor (11) comprising a cavity (12), said first reactor being configured to be placed inside a liquid bath (13) comprising at least one bacterial population, such that, when the first reactor (11) is in contact with the liquid bath (13), a biofilm is formed around the cavity (12);an injector (7) for injecting the CO- and / or H2-containing gas (8) into the cavity (12), the biofilm being capable of carrying out a biological conversion of the CO- and / or H2-containing gas into methane;a first CO2 injection device (14), configured to inject CO2 gas (9) into the liquid bath (13).

The utility model discloses a soil flora culture device with a split type bottom double-spiral belt and a reverse screw, and relates to a stirring device. The barrel bottom and the barrel body are mounted in the base; the lower end of the barrel body is communicated with the barrel bottom; the connecting cover is fixedly connected with the upper end of the barrel body; the barrel cover is connected with the connecting cover through a connecting device; the driving mechanism is fixed in the base; the stirring mechanism is mounted in the barrel body; two ends of the stirring mechanism are respectively connected with the barrel cover and the driving mechanism. According to the utility model, through the mutual matching among the outer-ring helical ribbon, the inner-ring helical ribbon and the connecting rod, the mixing and stirring of soil in the vertical direction, the cutting and mixing and stirring in the horizontal direction, the scraping of the inner wall of the barrel body and the filling of soil in a central hole in the barrel body can be realized; the stirring mechanism not only can realize rotary stirring around the central shaft, but also has upward thrust of a stirring rod and downward pressure of a helical ribbon, soil with the humidity of about 45% can be uniformly stirred, even large soil can be uniformly stirred, the stirring effect is strong, and the efficiency is high.

The invention relates to a colon-targeted micro-ecological regulation composition. The colon-targeted micro-ecological regulation composition is prepared by compounding the following functional components in percentage by weight: 5-95% of a component A, 1-15% of a component B and 4-94% of a component C, the component A is resistant starch, the polymerization degree DP is greater than or equal to 15, and the crystallinity is greater than or equal to 35%; the component B is probiotics and is embedded through a complex coacervation method or an extrusion-gel method, the embedding rate is larger than or equal to 75%, and the particle size is 200-800 microns; the survival rate is greater than or equal to 60% after treatment in simulated gastric juice for 2 hours, and the survival rate is greater than or equal to 50% after treatment in simulated intestinal juice for 4 hours; the component C comprises functional nutritional auxiliary materials, the functional nutritional auxiliary materials comprise prebiotics and a medicinal and edible composition, and the prebiotics comprise rapid fermentation type prebiotics, medium-speed fermentation type prebiotics, slow fermentation type prebiotics and a non-fermentation type water-holding expansion component. By constructing a carbon source slow release-flora orientation-metabolism regulation ternary interaction collaborative network, accurate regulation and multi-target metabolism intervention of the whole digestive tract are realized.

The application discloses an aerobic-anaerobic bacteria combined coal pretreatment biological gas production device, which comprises a gas blowing system, a bacterial population pre-culturing system, an aerobic-anaerobic biological combined pretreatment and gas production system and a gas-liquid two-phase collection and monitoring system. The application also discloses a gas production method of the gas production device. The application takes coal as a substrate, and under suitable conditions, carries out culture and optimization of a methanogenic bacterial population, and simulates indoor biological gas production effects under different coal rank, bacterial type, combined pretreatment mode, treatment time and metabolic environment conditions. The application adopts an integrated combined processor to combine aerobic bacteria and anaerobic bacteria for pretreating coal, so that coal-based biological gas production can be safely and efficiently carried out, and the application has important environmental benefits and broad application prospect.

This disclosure provides a method of treating gastrointestinal inflammation such as inflammatory bowel disease (IBS) with a bacteriophage containing composition. The bacteriophage containing composition can be used as prebiotic supplementation and support of the gastrointestinal microflora. This disclosure relates to compositions that comprise one or more types of bacteriophage and methods of using such bacteriophages as a prebiotic to promote the growth of beneficial bacteria by decreasing harmful bacterial populations and releasing nutrients into the environment for good bacteria in the digestive system of an individual.

Provided herein, inter alia, are compositions of organic acid-producing microorganisms and methods of making and using the same to inhibit pathogenic bacterial populations in the gastrointestinal tracts of an animal and additionally promote improvement of one or more metrics in an animal, such as increased bodyweight gain, decreased feed conversion ratio (FCR), improved gut barrier integrity, reduced mortality, reduced pathogen infection, and reduced pathogen shedding in feces.

The present invention provides a method for predicting the tendency of weight loss in Equus animals by evaluating multiple markers in a sample obtained from an Equus animal. Such markers include fermentation products and / or their metabolites, such as volatile fatty acids, bacterial population diversity indicators, and the abundance / relative abundance of specific bacterial taxa.

A method and system for increasing beneficial bacteria populations in a subject's oral cavity and decreasing pathogenic bacteria to address a variety of diseases and conditions, thereby enhancing a person's oral and overall health, including through the use of oral strips that adhere to surfaces in the oral cavity and that include at least one of xylitol, Lachnospira, Veillonella, Faecalibacterium and / or Rothia bacteria, including bacteria transformed via a CRISPR system. Certain embodiments employ light to modify oral cavity bacterial populations, including the use of bioluminescent oral strips that promote the growth of beneficial bacteria and decrease pathogenic bacteria populations.

The application discloses a salt-tolerant composite bacterial community, a preparation method thereof and application of the composite bacterial community in high-salt oil sludge remediation. Halomonas venusta The composite bacterial community comprises salt bacillus Acinetobacter calcoaceticus C. acnes CCTCC KB 20081402, and pseudomonas aeruginosa Pseudomonas aeruginosa CCTCC AB 2010174. The application utilizes H. venusta The Ectoine secretion characteristic of the DSM 4743 provides osmotic pressure protection for non-salt-tolerant strains in the composite bacterial community, a Ectoine secretion-transmission-degradation synergistic mechanism is constructed, and the degradation efficiency of petroleum hydrocarbon in a high-salt environment is greatly improved. Experiments show that the petroleum hydrocarbon degradation rate of the composite bacterial community in high-salt oil sludge with a salt content of 35 g / L can reach 43.12% within 7 days. The application is suitable for oil sludge remediation in high-salt environments such as offshore oil fields and saline-alkali soil petroleum contaminated sites, and has important environmental and economic benefits.

The invention features E. faecalis phage-derived antimicrobial agent (efagins). The invention also features compositions and methods of using the efagins to treat bacterial infections.

The invention discloses an in-situ water treatment and micronutrient slow release device for a seawater aquarium, and relates to the technical field of aquarium maintenance equipment. The device comprises a probiotic microorganism attachment framework and a plurality of layers of slow-release nutrition balls. The framework is composed of an upper biochemical filter bin and a lower slow release bin, and the upper filter bin is of a dense honeycomb structure so as to provide a huge microorganism attachment surface area; the lower slow-release bin is a drawable drawer with micropores, and the top surface of the lower slow-release bin is closed to protect the upper flora. The slow-release ball adopts a three-layer layered design, wherein the outer layer is a quick start and flora colonization layer; the middle layer contains polyhydroxyalkanoate PHA or PHBV and a microelement chelate, and is used as an algae phase competition and microelement supplement layer; and the inner layer contains a plurality of amino acids and astaxanthin and is used as a coral nutrition and homeostasis maintaining layer. The device is specially designed for the seawater coral jar, nutrient substances are released in a time sequence, the problems of element fluctuation and coral maintenance are solved, and efficient and stable maintenance of water quality is achieved.

The invention belongs to the technical field of safety evaluation of gene editing plants, and particularly relates to a method for evaluating the influence of a gene editing plant on biodiversity. According to the method, the influence of the gene editing plant on community diversity of animals, plants and microorganisms on the ground and underground is comprehensively and accurately evaluated from the influence of the gene editing plant on arthropod community structures, the influence of the gene editing plant on occurrence of main weeds and the influence of the gene editing plant on the number of soil bacterial colonies and the community structures, and a parameter evaluation method is established; compared with a non-gene editing plant, the gene editing plant has no obvious influence on the community structure of arthropods in the field, the weed occurrence condition, the number of soil bacterial communities and the community structure. According to the evaluation method provided by the invention, the environmental safety risk of the gene editing plant can be systematically evaluated, and a solid technical support is provided for safety evaluation before commercialized planting of the gene editing plant.

The present application provides the use of a nanocomposite with a core-shell structure. Specifically, the present application provides the use of a nanocomposite with a core-shell structure in the preparation of a drug for preventing and / or treating Clostridium difficile infection and recurrent diseases caused thereby, wherein the nanocomposite comprises: ZnO nanoparticles as a core; mesoporous silica as a shell coated on the surface of the ZnO nanoparticles; an antibiotic loaded in the pore channel of the mesoporous silica; hyperbranched polylysine modified on the surface of the mesoporous silica; and a pH-responsive coating material as the outermost layer. The nanocomposite provided by the present application can achieve precise targeted release of the drug in the colon, and through the sequential and synergistic mechanism of "membrane breaking-sterilization-spore inhibition-repair", it can simultaneously solve multiple factors leading to CDI recurrence such as biofilm protection, spore storage, bacterial population destruction, mucosal damage, etc., and improve the treatment effect.

The present invention provides a sample preparation device, comprising: a substrate, one side surface of the substrate being a plane, and a sample storage portion being formed on the side surface of the substrate; a scraper and a base, the scraper being detachably mounted on the base, a through cavity being defined between the scraper and the base, the substrate being movably disposed in the cavity, the lower surface of the scraper being a plane and facing the side surface of the substrate; a support portion being provided on the base, the support portion being used to mount a sampling rod, the sampling rod being rotatable on the support portion about a first axis, the first axis being perpendicular to the direction of movement of the substrate, and the first axis being parallel to the side surface of the substrate. When the sample on the sample storage portion passes through the scraper, the scraper can flatten the sample, making the sample uniform, thereby avoiding sample preparation differences caused by different people preparing the sample, and the sample can be applied to a predetermined position, resulting in a uniform distribution of bacterial populations and improving the accuracy of the test results.

Provided herein, inter alia, are compositions of short chain fatty acid (SCFA)-producing microorganisms and methods of making and using the same to inhibit pathogenic bacterial populations in the gastrointestinal tracts of an animal and additionally promote improvement of one or more metrics in an animal, such as increased bodyweight gain, decreased feed conversion ratio (FCR), improved gut barrier integrity, reduced mortality, reduced pathogen infection, and reduced pathogen shedding in feces.