107 results about "Bacterial population" patented technology

The invention relates to the technical field of biological process intelligent control, and discloses a pomegranate leaf fermentation process optimization and intelligent control system based on digital twinning. According to the system, the Internet of Things and a visual sensor are integrated through a multi-source data sensing module, so that synchronous acquisition and fusion of temperature, pH, dissolved oxygen, flora density and image data are realized; the intelligent data preprocessing module extracts high-quality features by adopting a self-adaptive filtering technology; the digital twin simulation engine module drives the virtual model to dynamically update based on real-time data; the deep prediction module accurately predicts the fermentation trend through a deep learning algorithm; the intelligent diagnosis module realizes abnormal root cause analysis by using a knowledge graph; the reinforcement learning optimization module autonomously generates an optimal control strategy based on the diagnosis result; and the self-adaptive execution module accurately regulates and controls fermentation parameters. According to the system, multi-dimensional perception, high-precision prediction and adaptive optimization control of the fermentation process are realized, and the accuracy and stability of process control are effectively improved.

The utility model discloses a soil flora culture device with a split type bottom double-spiral belt and a reverse screw, and relates to a stirring device. The barrel bottom and the barrel body are mounted in the base; the lower end of the barrel body is communicated with the barrel bottom; the connecting cover is fixedly connected with the upper end of the barrel body; the barrel cover is connected with the connecting cover through a connecting device; the driving mechanism is fixed in the base; the stirring mechanism is mounted in the barrel body; two ends of the stirring mechanism are respectively connected with the barrel cover and the driving mechanism. According to the utility model, through the mutual matching among the outer-ring helical ribbon, the inner-ring helical ribbon and the connecting rod, the mixing and stirring of soil in the vertical direction, the cutting and mixing and stirring in the horizontal direction, the scraping of the inner wall of the barrel body and the filling of soil in a central hole in the barrel body can be realized; the stirring mechanism not only can realize rotary stirring around the central shaft, but also has upward thrust of a stirring rod and downward pressure of a helical ribbon, soil with the humidity of about 45% can be uniformly stirred, even large soil can be uniformly stirred, the stirring effect is strong, and the efficiency is high.

The invention relates to a colon-targeted micro-ecological regulation composition. The colon-targeted micro-ecological regulation composition is prepared by compounding the following functional components in percentage by weight: 5-95% of a component A, 1-15% of a component B and 4-94% of a component C, the component A is resistant starch, the polymerization degree DP is greater than or equal to 15, and the crystallinity is greater than or equal to 35%; the component B is probiotics and is embedded through a complex coacervation method or an extrusion-gel method, the embedding rate is larger than or equal to 75%, and the particle size is 200-800 microns; the survival rate is greater than or equal to 60% after treatment in simulated gastric juice for 2 hours, and the survival rate is greater than or equal to 50% after treatment in simulated intestinal juice for 4 hours; the component C comprises functional nutritional auxiliary materials, the functional nutritional auxiliary materials comprise prebiotics and a medicinal and edible composition, and the prebiotics comprise rapid fermentation type prebiotics, medium-speed fermentation type prebiotics, slow fermentation type prebiotics and a non-fermentation type water-holding expansion component. By constructing a carbon source slow release-flora orientation-metabolism regulation ternary interaction collaborative network, accurate regulation and multi-target metabolism intervention of the whole digestive tract are realized.

The application discloses an aerobic-anaerobic bacteria combined coal pretreatment biological gas production device, which comprises a gas blowing system, a bacterial population pre-culturing system, an aerobic-anaerobic biological combined pretreatment and gas production system and a gas-liquid two-phase collection and monitoring system. The application also discloses a gas production method of the gas production device. The application takes coal as a substrate, and under suitable conditions, carries out culture and optimization of a methanogenic bacterial population, and simulates indoor biological gas production effects under different coal rank, bacterial type, combined pretreatment mode, treatment time and metabolic environment conditions. The application adopts an integrated combined processor to combine aerobic bacteria and anaerobic bacteria for pretreating coal, so that coal-based biological gas production can be safely and efficiently carried out, and the application has important environmental benefits and broad application prospect.

This disclosure provides a method of treating gastrointestinal inflammation such as inflammatory bowel disease (IBS) with a bacteriophage containing composition. The bacteriophage containing composition can be used as prebiotic supplementation and support of the gastrointestinal microflora. This disclosure relates to compositions that comprise one or more types of bacteriophage and methods of using such bacteriophages as a prebiotic to promote the growth of beneficial bacteria by decreasing harmful bacterial populations and releasing nutrients into the environment for good bacteria in the digestive system of an individual.

Provided herein, inter alia, are compositions of organic acid-producing microorganisms and methods of making and using the same to inhibit pathogenic bacterial populations in the gastrointestinal tracts of an animal and additionally promote improvement of one or more metrics in an animal, such as increased bodyweight gain, decreased feed conversion ratio (FCR), improved gut barrier integrity, reduced mortality, reduced pathogen infection, and reduced pathogen shedding in feces.

The application discloses a salt-tolerant composite bacterial community, a preparation method thereof and application of the composite bacterial community in high-salt oil sludge remediation. Halomonas venusta The composite bacterial community comprises salt bacillus Acinetobacter calcoaceticus C. acnes CCTCC KB 20081402, and pseudomonas aeruginosa Pseudomonas aeruginosa CCTCC AB 2010174. The application utilizes H. venusta The Ectoine secretion characteristic of the DSM 4743 provides osmotic pressure protection for non-salt-tolerant strains in the composite bacterial community, a Ectoine secretion-transmission-degradation synergistic mechanism is constructed, and the degradation efficiency of petroleum hydrocarbon in a high-salt environment is greatly improved. Experiments show that the petroleum hydrocarbon degradation rate of the composite bacterial community in high-salt oil sludge with a salt content of 35 g / L can reach 43.12% within 7 days. The application is suitable for oil sludge remediation in high-salt environments such as offshore oil fields and saline-alkali soil petroleum contaminated sites, and has important environmental and economic benefits.

The invention discloses an in-situ water treatment and micronutrient slow release device for a seawater aquarium, and relates to the technical field of aquarium maintenance equipment. The device comprises a probiotic microorganism attachment framework and a plurality of layers of slow-release nutrition balls. The framework is composed of an upper biochemical filter bin and a lower slow release bin, and the upper filter bin is of a dense honeycomb structure so as to provide a huge microorganism attachment surface area; the lower slow-release bin is a drawable drawer with micropores, and the top surface of the lower slow-release bin is closed to protect the upper flora. The slow-release ball adopts a three-layer layered design, wherein the outer layer is a quick start and flora colonization layer; the middle layer contains polyhydroxyalkanoate PHA or PHBV and a microelement chelate, and is used as an algae phase competition and microelement supplement layer; and the inner layer contains a plurality of amino acids and astaxanthin and is used as a coral nutrition and homeostasis maintaining layer. The device is specially designed for the seawater coral jar, nutrient substances are released in a time sequence, the problems of element fluctuation and coral maintenance are solved, and efficient and stable maintenance of water quality is achieved.

The present application provides the use of a nanocomposite with a core-shell structure. Specifically, the present application provides the use of a nanocomposite with a core-shell structure in the preparation of a drug for preventing and / or treating Clostridium difficile infection and recurrent diseases caused thereby, wherein the nanocomposite comprises: ZnO nanoparticles as a core; mesoporous silica as a shell coated on the surface of the ZnO nanoparticles; an antibiotic loaded in the pore channel of the mesoporous silica; hyperbranched polylysine modified on the surface of the mesoporous silica; and a pH-responsive coating material as the outermost layer. The nanocomposite provided by the present application can achieve precise targeted release of the drug in the colon, and through the sequential and synergistic mechanism of "membrane breaking-sterilization-spore inhibition-repair", it can simultaneously solve multiple factors leading to CDI recurrence such as biofilm protection, spore storage, bacterial population destruction, mucosal damage, etc., and improve the treatment effect.

The application discloses a granular fertilizer for improving saline-alkali soil based on traditional Chinese medicine active matter cooperation and multi-stage endogenous controlled release, and a preparation method and application thereof. Fermented straw is used as a core organic carrier, and milk and a traditional Chinese medicine (Astragalus, licorice, honeysuckle and the like) active matter collection rich in amino acids and proteins are introduced innovatively in the fermentation process, so that the quality and biological activity of the carrier are improved significantly. The carrier can not only be dissolved quickly to create a high-activity microenvironment for functional microorganisms, but also cooperates with the traditional Chinese medicine active matter to build a whole-chain ecological restoration network covering soil chemical improvement, biological cycle acceleration, plant stress resistance induction and soil-borne disease prevention. In combination with a four-fold endogenous protection system of microorganisms, an internal intercommunication porous structure formed by low-temperature compression and quantitative regulation of the coating thickness, the functional bacterial population has an ultrahigh survival rate, the saline-alkali and biological obstacles are improved synchronously, and the nutrient intelligent release is realized.

The application relates to a microbial fermentation device for beef jerky processing and relates to the technical field of food fermentation equipment, and comprises a material conveying pipe. The application can accurately control the temperatures of the front section, the middle section and the rear section of the material conveying pipe by arranging three groups of heating components and uniformly distributed temperature sensors, can match the growth activity peak value of probiotics, can improve the proliferation efficiency of a bacterial population and the activity of probiotics, can avoid flavor substance imbalance, and can improve the heating efficiency and uniformity by adopting an opening design of a heat preservation shell and a spiral-wound heating pipe. Meanwhile, the heat loss is reduced by a protective shell and a thermal insulation aerogel, the internal components are protected, the perforation of spiral conveying blades is matched with a turnover plate, the beef jerky material mixing is promoted, the temperature accurate control in the beef jerky fermentation process, the uniform mixing of beef jerky material and the uniform distribution of bacterial liquid are realized, the fermentation efficiency and product flavor coordination are effectively improved, the fermentation period is shortened, and the product quality is improved.

The invention discloses a composite flora and activated mineral synergistic bio-organic fertilizer and a preparation method thereof. The preparation method comprises the following steps: crushing and mixing organic raw materials, adding a compound bacterium enzyme preparation, and sequentially carrying out two-stage temperature-controlled fermentation of high-temperature aerobic fermentation and medium-temperature curing to obtain a high-activity fermented material; the preparation method comprises the following steps: carrying out nano micropore activation treatment on monoclinoptilolite by using a hydrochloric acid solution to obtain activated zeolite with high specific surface area; and finally, mixing the fermented material with the activated zeolite in proportion, chelating at low temperature, granulating and drying to obtain the bio-organic fertilizer. Through composite flora construction, mineral nano activation and a step-by-step synergistic process, the effective viable count of the product reaches 2.0 billion CFU / g or above, the Pb < 2 + > adsorption rate exceeds 85%, and nutrient slow release and remarkable growth promoting effects are achieved. The product integrates the functions of soil improvement, disease inhibition, heavy metal restoration and nutrient slow release, and is suitable for ecological planting of various crops.

The invention relates to the technical field of fertilizers, and discloses a special fertilizer for saline-alkali soil soybeans and a preparation method thereof, and the method comprises the following steps: preparing a multi-layer coating structure, embedding a quick-acting chemical buffer in an outer layer, embedding a biological fermentation substrate in a middle layer, and loading an organic acid precursor substance in an inner layer; culturing and inoculating the growth-promoting rhizosphere flora; the gradient density granulation process is used for regulating and controlling the density distribution of fertilizer particles; and compounding the chelated trace elements. Through a biological-chemical synergistic regulation method, a continuous and stable pH regulation effect in a saline-alkali soil environment is achieved, the technical problems that a traditional single route is short in regulation effect and unstable in biological regulation function are solved, the nutrient absorption and utilization rate and saline-alkali stress resistance of the soybeans are remarkably improved, and the yield of the soybeans is increased. And an environment-friendly technical solution with high process practicability is provided for soybean planting in the saline-alkali soil.

The invention relates to the technical field of composite microbial fertilizer manufacturing, and discloses a composite microbial fertilizer synergist capable of cooperating with multiple beneficial strains and a production method thereof, and the production method comprises the following steps: mixing a hydrophilic temporary storage agent with a basic carrier, spraying critical water lower than a microbial activation threshold value, and uniformly stirring; inducing the surface gelling of the hydrophilic temporary storage agent by using water to form a viscous micronucleus; the preparation method comprises the following steps: firstly, adding a hydrophobic separant and a core flora, realizing spontaneous selective coating by utilizing a micronucleus viscous surface, and finally dispersing a pioneer flora into a matrix outside a coating structure. A microcosmic functional structure of particles is preset in a preparation process, so that the activation relationship of microorganisms after meeting water is competed by disordered synchronous resources, and the microcosmic functional structure of the particles is changed into the microcosmic functional structure of the particles, and the microcosmic functional structure of the particles is changed into the microcosmic functional structure of the particles; the method is converted into deterministic time sequence ecological niche relay, so that the advantage of diversity of strains in the formula can be exerted in practical application.

The invention provides a composite conditioner for acid soil remediation and application thereof, and belongs to the technical field of agricultural environment and soil remediation. The composite conditioner comprises a first component, a second component and a third component which are independently packaged, wherein the modified straw biochar in the first component is used as a'microcarrier 'and a'buffer', calcium magnesium ore powder containing high-activity calcium magnesium oxide is loaded, slow release of alkaline substances is achieved, local over-alkalinity and hardening risks are avoided, and the porosity and the fertilizer retention capacity are improved; the second component is compounded with high-molecular polymer hydrolytic polymaleic anhydride, polyaspartic acid and polyglutamic acid according to a specific proportion, and the synergistic chelation capacity to aluminum and manganese ions and the dispersion capacity to soil particles are obviously superior to those of any single component and other proportion combinations; the third component selects an acid-resistant compound strain and is supplemented with amino acid to serve as a nutrient substance for rapid proliferation of the acid-resistant compound strain, so that high survival rate, colonization success rate and functional expression efficiency of the exogenous flora in an acidic stress are ensured.

The invention belongs to the technical field of compound microbial fertilizer manufacturing, and discloses a compound functional flora fertilizer formula for cadmium-contaminated soil remediation, which comprises the following steps: atomizing a premixed solution containing a second functional flora vitrification protective agent and an ionic cross-linking agent, applying the atomized premixed solution to a wet organic matrix preloaded with multivalent cations, and carrying out osmotic pressure driving to prepare the compound functional flora fertilizer for cadmium-contaminated soil remediation. According to the method, when a glassy state protection structure is formed around the flora in situ, a gel network is formed at an interface to anchor the flora, microcosmic protection and firm anchoring of the core functional flora are synchronously completed in the wet mixing process of the fertilizer, irreversible physical damage caused by a traditional drying process to the strain is avoided, and the method is suitable for large-scale industrial production. The bacterial strain with strong function and weak environmental tolerance can be applied to the product with high survival rate, and the life rhythm of orderly release of functional flora is formed.

This invention provides a method for producing single-cell protein using a mixed bacterial culture, comprising the following steps: performing primary seed culture on methanogenic bacteria, *Tricholoma materata*, and *Glucosinolate methyl-eating bacteria* to obtain primary seed solutions; mixing the primary seed culture solutions evenly to obtain a mixed bacterial solution; inoculating the obtained mixed bacterial solution into a secondary seed culture medium for cultivation to obtain a secondary seed solution; inoculating the obtained secondary seed solution into a fermentation medium for scale-up cultivation to obtain a fermentation broth; separating the solid and liquid components of the obtained fermentation broth, harvesting the bacterial cells, and obtaining single-cell protein. This method for producing single-cell protein using a mixed bacterial culture improves the utilization rate of the carbon source methane and the yield of single-cell protein.

Provided herein are pharmaceutical compositions comprising a bacterial population. The bacterial population comprises one bacterial strains or at least two bacterial strains. Such pharmaceutical compositions can be administered to a subject for prevention and / or treatment of dysbiosis and dysbiosis associated conditions and diseases, such as infant gastrointestinal diseases.

The invention relates to the technical field of environmental biology, and discloses a short-cut nitrification-denitrification complex microbial inoculant and a preparation method thereof. The method includes; the method comprises the following steps: respectively culturing ammonia oxidizing bacteria, denitrifying bacteria and heterotrophic nitrification-denitrifying bacteria, mixing, and co-domesticating under a low dissolved oxygen condition to obtain a co-domesticated flora; and then adding into sodium alginate-polyvinyl alcohol, immobilizing by a calcium chloride-boric acid-glutaraldehyde system, and freeze-drying to obtain the complex microbial inoculant. The prepared complex microbial inoculant can stably realize synchronous short-cut nitrification and denitrification under the condition of low dissolved oxygen, is high in nitrite accumulation rate, remarkably improves the removal rate of ammonia nitrogen and total nitrogen, has the advantages of stable flora structure, high denitrification efficiency and low operation energy consumption, and is suitable for industrial production. The method can be widely applied to biological nitrogen treatment of urban sewage and high-ammonia-nitrogen industrial wastewater.

The present disclosure relates to a pectin-containing wastewater treatment method and a treatment device, the method comprising: inputting first pectin wastewater into a biological conversion tank, and performing biological conversion treatment on pectin in the first pectin wastewater based on a pectin-cellulose degradation mixed bacteria to obtain second pectin wastewater containing small molecular structure pectin; wherein the pectin-cellulose degradation mixed bacteria is a mixed bacterial population capable of simultaneously degrading pectin and cellulose, and the biological conversion treatment process is used to change the pectin structure without affecting the chemical oxygen demand corresponding to the first pectin wastewater; inputting the second pectin wastewater into a primary or multi-stage anaerobic reactor for anaerobic decomposition treatment to obtain wastewater with reduced organic matter concentration after pectin decomposition and biogas; and the biogas is used for input into a power supply end or an energy storage end for recycling. In the wastewater treatment process, in-situ treatment and relatively sufficient degradation of pectin are achieved, and the treatment load and treatment efficiency are improved.

The invention relates to the technical field of water treatment equipment, and discloses a fishpond and old fish tank transformation system which comprises a fish tank and a purification tank located on the lower portion of the fish tank, a purification tank, an MABR film, a water pump, filler, an air pump, an ultraviolet sterilizer and a siphon are arranged in the purification tank, the air pump is arranged at the bottom of the fish tank and connected with the MABR film, and the water pump is connected with the fish tank through a water conveying pipe. The purification tank comprises an aerobic bin and an anoxic bin, a filter tank, an aeration disc, an MABR membrane and filler are arranged in the purification tank, a plug flow machine and a photocatalytic oxidation device are arranged in the fish tank, and the photocatalytic oxidation device comprises a radiation lamp, a quartz tube, a light-proof shell and a photocatalytic medium. The filter material is prevented from being blocked and does not need to be frequently replaced or cleaned, a nitrification system is enhanced, the water quality in the fish tank is improved, and the stability of flora is kept.

The invention relates to a pesticide-fertilizer granule and a preparation method thereof, the granule is composed of a compound functional flora domesticated by saline-alkali gradient stress, an organic carrier prepared by fermenting agricultural and forestry wastes and livestock and poultry manure, and a chemical synergistic component, and the effective viable count is greater than or equal to 0.5 billion CFU / g. The preparation method comprises the following steps: (1) screening salt-dissolving, growth-promoting and stress-resistant composite flora from a saline-alkali soil sample; (2) performing high-temperature aerobic fermentation on forestry and agricultural residues and livestock and poultry manure to prepare a carrier; (3) inoculating the functional flora to the carrier by adopting a segmented inoculation process; and (4) extruding and granulating at a low temperature lower than 0 DEG C. The bottleneck that a traditional bacterial fertilizer is single in function, high in carrier cost, large in granulation viable bacterium loss and the like is broken through, a chemical modifier and bacterial fertilizer synergistic application mode is innovated for severe saline-alkali soil, the soil salt content is reduced by 25%-30%, the crop yield is increased by 15%-20%, the strain survival rate is larger than or equal to 75%, and a standard and low-cost solution is provided for green improvement of the saline-alkali soil.

The application discloses a kind of microbial complex bacterial population, microbial inoculum and application thereof, and is related to microorganism and application technical field.The microbial complex bacterial population includes Acinetobacter sichuanensis LHH12, Pseudomonas songnenensis SJ2-76, Diaphorobacter nitroreducens SYY4 and Thauera aminoaromatica SJ2-12.The microorganism for constructing complex bacterial population is obtained from semi-coke wastewater, and has good phenol tolerance and chemical oxygen demand (COD) degradation capacity, and can be used for purifying high-phenol wastewater, biological denitrification and phosphorus removal and degrading COD.The microbial complex bacterial population of the application can effectively improve the COD degradation rate in phenol ammonia wastewater and effectively reduce the treatment cost of high-phenol ammonia wastewater such as semi-coke without changing the original microbial community composition of wastewater through the synergistic effect between strains, which has great potential for promoting low-carbon green and sustainable development of coal chemical industry.

The current invention relates to a purified bacterial strain for improving plant growth and / or yield. The invention also relates to a bacterial population, a microbial active ingredient, an agriculturally active ingredient, a synthetic composition, and methods for improving plant growth and / or yield by improving a trait of agronomic importance in a plant and by conferring resistance to a plant pathogen infection.

The application belongs to the technical field of biological fermentation, and particularly relates to a dark fermentation mixed flora, a culture method thereof and application of the dark fermentation mixed flora in hydrogen production from waste oil reservoirs. In the application, a mixed flora capable of producing hydrogen by using waste oil reservoirs is disclosed, the mixed flora comprising Thermotoga hypogea, Thermotoga elfii, Thermus sp., Propionibacterium freudenreichii, Butyrivibrio fibrisolvens and Pseudomonas aeruginosa. The mixed flora can not only produce hydrogen by using crude oil as raw material, but also improve the flowability of the oil layer, improve the interface between the oil layer and the water layer, and crack heavy components in the crude oil, so that the oil layer can be fully utilized for fermentation hydrogen production, and the efficiency of hydrogen production by microorganisms using waste oil reservoirs is improved.

The present application is suitable for the field of bioremediation technology, and provides a bioremediation preparation for heavy oil pollution, which comprises an immobilized heavy oil degrading bacterial population preparation, an immobilized laccase preparation, a slow-release nitrogen and phosphorus nutrient preparation, and a rhamnolipid surfactant; the bioremediation preparation is a solid preparation. The present application also provides application of the bioremediation preparation in a heavy oil pollution intertidal zone. The bioremediation preparation has the advantages of not being easy to lose, fast bioremediation starting speed, being superior in competition with indigenous bacterial species, and good environmental tolerance, thereby overcoming the problems of slow starting speed and fast failure speed of traditional bioremediation preparations in remediation of heavy oil pollution intertidal zones.

The invention relates to an arsenic-gold dissociation optimization treatment device and method for arsenic-gold ore, and belongs to the technical field of gold ore treatment.The multiple sensors are arranged in a reaction chamber of the arsenic-gold dissociation optimization treatment device for the arsenic-gold ore, and multi-source data information is collected through the multiple sensors; a digital twinborn body is formed through the mechanism model layer, the data driving model layer and the high-fidelity simulation layer, so that process states under different control parameter combinations within a preset time are predicted in a virtual space, and the maximum arsenic oxidation rate and flora activity optimal interval are solved based on a genetic algorithm; and calculating an optimal control instruction set, and performing control optimization based on the optimal control instruction set. According to the method, the virtual-real interactive digital twinborn system is constructed, and the genetic algorithm is fused for dynamic optimization, so that accurate prediction and real-time optimization of the biological oxidation process are realized, the arsenic and gold dissociation degree in the arsenic-containing gold ore is improved, and the preparation purity of the gold ore is improved.

This invention discloses a method and system for predicting a subject's response to a therapy by: obtaining a first plurality of nucleic acid sequences targeting genomic DNA from a sample of the subject's gut; determining multiple genomic abundance values ​​for various gut bacteria from the nucleic acid sequences; and applying a model to the plurality of genomic abundance values ​​to obtain a prediction of the subject's response to the therapy as the output of the model.

The invention relates to the technical field of tea enzyme preparation, in particular to a Pu'er tea enzyme powder preparation method which comprises the steps of tea leaf pretreatment, fermentation liquor preparation, fermentation liquor filtration and drying for powder preparation. According to the Pu'er tea enzyme powder and the preparation method thereof, the Pu'er raw tea leaves, the sugar source, the EM bacterial powder, the citrus peel particles and the lemon peel particles are taken as raw materials, so that the flavor and the enzyme activity can be enhanced, multi-stage synergistic fermentation is realized, flora proliferation is promoted through natural fructose and cellulose, and the enzyme activity and the organic acid yield are improved; by combining the whole fermentation period, matched control of exhaust and pH value monitoring is realized according to flora activity in different stages, and the problem of out-of-control fermentation or infectious microbe pollution can be avoided; through high-pressure sterilization gauze filtration, pasteurization and centrifugal layering plate-frame pressure filtration treatment, the purity of a stock solution can be effectively guaranteed, and the survival rate of active ingredients such as enzymes, probiotics and polyphenols in the enzyme powder is effectively increased by adopting a low-temperature concentration and freeze-drying process.

The invention discloses a chloride ion response-composite flora double-shell microcapsule which comprises a chloride ion response shell layer, an outer bacterium-nutrition core material, an inner slow-release shell layer and an inner nutrition core material which are sequentially arranged from outside to inside, wherein the raw materials of the chloride ion response shell layer comprise a first response hydrogel material and LDHs; the outer-layer bacterium-nutrient core material comprises a bacterium-nutrient solution coated with acrylic resin; the inner slow-release shell layer is prepared from the following raw materials: sodium carboxymethyl cellulose and a second hydrogel material; and the inner layer nutrition core material comprises waterborne polyurethane loaded carbon and nitrogen source nutrition ingredients and glycerol. According to the invention, a cooperative mechanism of chloride ion targeted triggering and two-stage repairing regulation is adopted, so that the problems of response lag, single repairing rhythm and the like in the existing microbial repairing technology can be effectively solved; when the microcapsule is applied to preparation of anti-crack concrete, excellent crack self-repairing performance can be shown, and the service life of the concrete in high-erosion environments such as coastal soil and saline soil can be remarkably prolonged.