157 results about "Bacterial population" patented technology

The invention relates to the technical field of organic fertilizer production, in particular to a bio-organic fertilizer production process data system and an extraction method. The method comprises the following steps: acquiring a raw material information set and a process parameter set; calculating a reference flora rate based on microbial activity characteristics of the raw material information set; marking a time sequence timestamp for the raw material information set and the process parameter set, and constructing a fermentation behavior track; analyzing the microbial activity characteristics of the fermentation behavior track, and calculating the flora reaction speed; therefore, by constructing a multi-source heterogeneous data-driven fermentation behavior track and quality mapping system, the problems of data isolation, state recognition lag and process deviation and incapability of accurate positioning in traditional bio-organic fertilizer production are solved, and the intelligent diagnosis capability and quality controllability of the production process are improved.

The invention relates to methods, uses, systems, arrays, engineered nucleotide sequences and vectors for inhibiting bacterial population growth or for altering the relative ratio of sub-populations of first and second bacteria in a mixed population of bacteria. The invention is particularly useful, for example, for treatment of microbes such as for environmental, medical, food and beverage use. The invention relates inter alia to methods of controlling microbiologically influenced corrosion (MIC) or biofouling of a substrate or fluid in an industrial or domestic system.

The invention relates to methods, uses, systems, arrays, engineered nucleotide sequences and vectors for inhibiting bacterial population growth or for altering the relative ratio of sub-populations of first and second bacteria in a mixed population of bacteria. The invention is particularly useful, for example, for treatment of microbes such as for environmental, medical, food and beverage use. The invention relates inter alia to methods of controlling microbiologically influenced corrosion (MIC) or biofouling of a substrate or fluid in an industrial or domestic system.

The invention relates to the technical field of biological process intelligent control, and discloses a pomegranate leaf fermentation process optimization and intelligent control system based on digital twinning. According to the system, the Internet of Things and a visual sensor are integrated through a multi-source data sensing module, so that synchronous acquisition and fusion of temperature, pH, dissolved oxygen, flora density and image data are realized; the intelligent data preprocessing module extracts high-quality features by adopting a self-adaptive filtering technology; the digital twin simulation engine module drives the virtual model to dynamically update based on real-time data; the deep prediction module accurately predicts the fermentation trend through a deep learning algorithm; the intelligent diagnosis module realizes abnormal root cause analysis by using a knowledge graph; the reinforcement learning optimization module autonomously generates an optimal control strategy based on the diagnosis result; and the self-adaptive execution module accurately regulates and controls fermentation parameters. According to the system, multi-dimensional perception, high-precision prediction and adaptive optimization control of the fermentation process are realized, and the accuracy and stability of process control are effectively improved.

The invention relates to methods, uses, systems, arrays, engineered nucleotide sequences and vectors for inhibiting bacterial population growth or for altering the relative ratio of sub-populations of first and second bacteria in a mixed population of bacteria. The invention is particularly useful, for example, for treatment of microbes such as for environmental, medical, food and beverage use. The invention relates inter alia to methods of controlling microbiologically influenced corrosion (MIC) or biofouling of a substrate or fluid in an industrial or domestic system.

Provided are compositions and methods for selectively reducing the amount of antibiotic resistant and / or virulent bacteria in a mixed bacteria population, or for reducing any other type of unwanted bacteria in a mixed bacteria population. The compositions and methods involve targeting bacteria that are differentiated from other members of the population by at least one unique clustered regularly interspaced short palindromic repeats (CRISPR) targeted DNA sequence. The compositions and methods can be readily adapted to target any bacteria or any bacteria plasmid, or both.

The utility model discloses a soil flora culture device with a split type bottom double-spiral belt and a reverse screw, and relates to a stirring device. The barrel bottom and the barrel body are mounted in the base; the lower end of the barrel body is communicated with the barrel bottom; the connecting cover is fixedly connected with the upper end of the barrel body; the barrel cover is connected with the connecting cover through a connecting device; the driving mechanism is fixed in the base; the stirring mechanism is mounted in the barrel body; two ends of the stirring mechanism are respectively connected with the barrel cover and the driving mechanism. According to the utility model, through the mutual matching among the outer-ring helical ribbon, the inner-ring helical ribbon and the connecting rod, the mixing and stirring of soil in the vertical direction, the cutting and mixing and stirring in the horizontal direction, the scraping of the inner wall of the barrel body and the filling of soil in a central hole in the barrel body can be realized; the stirring mechanism not only can realize rotary stirring around the central shaft, but also has upward thrust of a stirring rod and downward pressure of a helical ribbon, soil with the humidity of about 45% can be uniformly stirred, even large soil can be uniformly stirred, the stirring effect is strong, and the efficiency is high.

The invention relates to a colon-targeted micro-ecological regulation composition. The colon-targeted micro-ecological regulation composition is prepared by compounding the following functional components in percentage by weight: 5-95% of a component A, 1-15% of a component B and 4-94% of a component C, the component A is resistant starch, the polymerization degree DP is greater than or equal to 15, and the crystallinity is greater than or equal to 35%; the component B is probiotics and is embedded through a complex coacervation method or an extrusion-gel method, the embedding rate is larger than or equal to 75%, and the particle size is 200-800 microns; the survival rate is greater than or equal to 60% after treatment in simulated gastric juice for 2 hours, and the survival rate is greater than or equal to 50% after treatment in simulated intestinal juice for 4 hours; the component C comprises functional nutritional auxiliary materials, the functional nutritional auxiliary materials comprise prebiotics and a medicinal and edible composition, and the prebiotics comprise rapid fermentation type prebiotics, medium-speed fermentation type prebiotics, slow fermentation type prebiotics and a non-fermentation type water-holding expansion component. By constructing a carbon source slow release-flora orientation-metabolism regulation ternary interaction collaborative network, accurate regulation and multi-target metabolism intervention of the whole digestive tract are realized.

The application discloses an aerobic-anaerobic bacteria combined coal pretreatment biological gas production device, which comprises a gas blowing system, a bacterial population pre-culturing system, an aerobic-anaerobic biological combined pretreatment and gas production system and a gas-liquid two-phase collection and monitoring system. The application also discloses a gas production method of the gas production device. The application takes coal as a substrate, and under suitable conditions, carries out culture and optimization of a methanogenic bacterial population, and simulates indoor biological gas production effects under different coal rank, bacterial type, combined pretreatment mode, treatment time and metabolic environment conditions. The application adopts an integrated combined processor to combine aerobic bacteria and anaerobic bacteria for pretreating coal, so that coal-based biological gas production can be safely and efficiently carried out, and the application has important environmental benefits and broad application prospect.

This disclosure provides a method of treating gastrointestinal inflammation such as inflammatory bowel disease (IBS) with a bacteriophage containing composition. The bacteriophage containing composition can be used as prebiotic supplementation and support of the gastrointestinal microflora. This disclosure relates to compositions that comprise one or more types of bacteriophage and methods of using such bacteriophages as a prebiotic to promote the growth of beneficial bacteria by decreasing harmful bacterial populations and releasing nutrients into the environment for good bacteria in the digestive system of an individual.

Provided herein, inter alia, are compositions of organic acid-producing microorganisms and methods of making and using the same to inhibit pathogenic bacterial populations in the gastrointestinal tracts of an animal and additionally promote improvement of one or more metrics in an animal, such as increased bodyweight gain, decreased feed conversion ratio (FCR), improved gut barrier integrity, reduced mortality, reduced pathogen infection, and reduced pathogen shedding in feces.

The present invention provides a method for predicting the tendency of weight loss in Equus animals by evaluating multiple markers in a sample obtained from an Equus animal. Such markers include fermentation products and / or their metabolites, such as volatile fatty acids, bacterial population diversity indicators, and the abundance / relative abundance of specific bacterial taxa.

The application discloses a salt-tolerant composite bacterial community, a preparation method thereof and application of the composite bacterial community in high-salt oil sludge remediation. Halomonas venusta The composite bacterial community comprises salt bacillus Acinetobacter calcoaceticus C. acnes CCTCC KB 20081402, and pseudomonas aeruginosa Pseudomonas aeruginosa CCTCC AB 2010174. The application utilizes H. venusta The Ectoine secretion characteristic of the DSM 4743 provides osmotic pressure protection for non-salt-tolerant strains in the composite bacterial community, a Ectoine secretion-transmission-degradation synergistic mechanism is constructed, and the degradation efficiency of petroleum hydrocarbon in a high-salt environment is greatly improved. Experiments show that the petroleum hydrocarbon degradation rate of the composite bacterial community in high-salt oil sludge with a salt content of 35 g / L can reach 43.12% within 7 days. The application is suitable for oil sludge remediation in high-salt environments such as offshore oil fields and saline-alkali soil petroleum contaminated sites, and has important environmental and economic benefits.

The invention features E. faecalis phage-derived antimicrobial agent (efagins). The invention also features compositions and methods of using the efagins to treat bacterial infections.

The invention discloses an in-situ water treatment and micronutrient slow release device for a seawater aquarium, and relates to the technical field of aquarium maintenance equipment. The device comprises a probiotic microorganism attachment framework and a plurality of layers of slow-release nutrition balls. The framework is composed of an upper biochemical filter bin and a lower slow release bin, and the upper filter bin is of a dense honeycomb structure so as to provide a huge microorganism attachment surface area; the lower slow-release bin is a drawable drawer with micropores, and the top surface of the lower slow-release bin is closed to protect the upper flora. The slow-release ball adopts a three-layer layered design, wherein the outer layer is a quick start and flora colonization layer; the middle layer contains polyhydroxyalkanoate PHA or PHBV and a microelement chelate, and is used as an algae phase competition and microelement supplement layer; and the inner layer contains a plurality of amino acids and astaxanthin and is used as a coral nutrition and homeostasis maintaining layer. The device is specially designed for the seawater coral jar, nutrient substances are released in a time sequence, the problems of element fluctuation and coral maintenance are solved, and efficient and stable maintenance of water quality is achieved.

The invention belongs to the technical field of safety evaluation of gene editing plants, and particularly relates to a method for evaluating the influence of a gene editing plant on biodiversity. According to the method, the influence of the gene editing plant on community diversity of animals, plants and microorganisms on the ground and underground is comprehensively and accurately evaluated from the influence of the gene editing plant on arthropod community structures, the influence of the gene editing plant on occurrence of main weeds and the influence of the gene editing plant on the number of soil bacterial colonies and the community structures, and a parameter evaluation method is established; compared with a non-gene editing plant, the gene editing plant has no obvious influence on the community structure of arthropods in the field, the weed occurrence condition, the number of soil bacterial communities and the community structure. According to the evaluation method provided by the invention, the environmental safety risk of the gene editing plant can be systematically evaluated, and a solid technical support is provided for safety evaluation before commercialized planting of the gene editing plant.

The present application provides the use of a nanocomposite with a core-shell structure. Specifically, the present application provides the use of a nanocomposite with a core-shell structure in the preparation of a drug for preventing and / or treating Clostridium difficile infection and recurrent diseases caused thereby, wherein the nanocomposite comprises: ZnO nanoparticles as a core; mesoporous silica as a shell coated on the surface of the ZnO nanoparticles; an antibiotic loaded in the pore channel of the mesoporous silica; hyperbranched polylysine modified on the surface of the mesoporous silica; and a pH-responsive coating material as the outermost layer. The nanocomposite provided by the present application can achieve precise targeted release of the drug in the colon, and through the sequential and synergistic mechanism of "membrane breaking-sterilization-spore inhibition-repair", it can simultaneously solve multiple factors leading to CDI recurrence such as biofilm protection, spore storage, bacterial population destruction, mucosal damage, etc., and improve the treatment effect.

The present invention provides a sample preparation device, comprising: a substrate, one side surface of the substrate being a plane, and a sample storage portion being formed on the side surface of the substrate; a scraper and a base, the scraper being detachably mounted on the base, a through cavity being defined between the scraper and the base, the substrate being movably disposed in the cavity, the lower surface of the scraper being a plane and facing the side surface of the substrate; a support portion being provided on the base, the support portion being used to mount a sampling rod, the sampling rod being rotatable on the support portion about a first axis, the first axis being perpendicular to the direction of movement of the substrate, and the first axis being parallel to the side surface of the substrate. When the sample on the sample storage portion passes through the scraper, the scraper can flatten the sample, making the sample uniform, thereby avoiding sample preparation differences caused by different people preparing the sample, and the sample can be applied to a predetermined position, resulting in a uniform distribution of bacterial populations and improving the accuracy of the test results.

Provided herein, inter alia, are compositions of short chain fatty acid (SCFA)-producing microorganisms and methods of making and using the same to inhibit pathogenic bacterial populations in the gastrointestinal tracts of an animal and additionally promote improvement of one or more metrics in an animal, such as increased bodyweight gain, decreased feed conversion ratio (FCR), improved gut barrier integrity, reduced mortality, reduced pathogen infection, and reduced pathogen shedding in feces.

The application discloses a granular fertilizer for improving saline-alkali soil based on traditional Chinese medicine active matter cooperation and multi-stage endogenous controlled release, and a preparation method and application thereof. Fermented straw is used as a core organic carrier, and milk and a traditional Chinese medicine (Astragalus, licorice, honeysuckle and the like) active matter collection rich in amino acids and proteins are introduced innovatively in the fermentation process, so that the quality and biological activity of the carrier are improved significantly. The carrier can not only be dissolved quickly to create a high-activity microenvironment for functional microorganisms, but also cooperates with the traditional Chinese medicine active matter to build a whole-chain ecological restoration network covering soil chemical improvement, biological cycle acceleration, plant stress resistance induction and soil-borne disease prevention. In combination with a four-fold endogenous protection system of microorganisms, an internal intercommunication porous structure formed by low-temperature compression and quantitative regulation of the coating thickness, the functional bacterial population has an ultrahigh survival rate, the saline-alkali and biological obstacles are improved synchronously, and the nutrient intelligent release is realized.

The application relates to a microbial fermentation device for beef jerky processing and relates to the technical field of food fermentation equipment, and comprises a material conveying pipe. The application can accurately control the temperatures of the front section, the middle section and the rear section of the material conveying pipe by arranging three groups of heating components and uniformly distributed temperature sensors, can match the growth activity peak value of probiotics, can improve the proliferation efficiency of a bacterial population and the activity of probiotics, can avoid flavor substance imbalance, and can improve the heating efficiency and uniformity by adopting an opening design of a heat preservation shell and a spiral-wound heating pipe. Meanwhile, the heat loss is reduced by a protective shell and a thermal insulation aerogel, the internal components are protected, the perforation of spiral conveying blades is matched with a turnover plate, the beef jerky material mixing is promoted, the temperature accurate control in the beef jerky fermentation process, the uniform mixing of beef jerky material and the uniform distribution of bacterial liquid are realized, the fermentation efficiency and product flavor coordination are effectively improved, the fermentation period is shortened, and the product quality is improved.

The invention discloses a composite flora and activated mineral synergistic bio-organic fertilizer and a preparation method thereof. The preparation method comprises the following steps: crushing and mixing organic raw materials, adding a compound bacterium enzyme preparation, and sequentially carrying out two-stage temperature-controlled fermentation of high-temperature aerobic fermentation and medium-temperature curing to obtain a high-activity fermented material; the preparation method comprises the following steps: carrying out nano micropore activation treatment on monoclinoptilolite by using a hydrochloric acid solution to obtain activated zeolite with high specific surface area; and finally, mixing the fermented material with the activated zeolite in proportion, chelating at low temperature, granulating and drying to obtain the bio-organic fertilizer. Through composite flora construction, mineral nano activation and a step-by-step synergistic process, the effective viable count of the product reaches 2.0 billion CFU / g or above, the Pb < 2 + > adsorption rate exceeds 85%, and nutrient slow release and remarkable growth promoting effects are achieved. The product integrates the functions of soil improvement, disease inhibition, heavy metal restoration and nutrient slow release, and is suitable for ecological planting of various crops.

The invention relates to the technical field of fertilizers, and discloses a special fertilizer for saline-alkali soil soybeans and a preparation method thereof, and the method comprises the following steps: preparing a multi-layer coating structure, embedding a quick-acting chemical buffer in an outer layer, embedding a biological fermentation substrate in a middle layer, and loading an organic acid precursor substance in an inner layer; culturing and inoculating the growth-promoting rhizosphere flora; the gradient density granulation process is used for regulating and controlling the density distribution of fertilizer particles; and compounding the chelated trace elements. Through a biological-chemical synergistic regulation method, a continuous and stable pH regulation effect in a saline-alkali soil environment is achieved, the technical problems that a traditional single route is short in regulation effect and unstable in biological regulation function are solved, the nutrient absorption and utilization rate and saline-alkali stress resistance of the soybeans are remarkably improved, and the yield of the soybeans is increased. And an environment-friendly technical solution with high process practicability is provided for soybean planting in the saline-alkali soil.

The invention relates to the technical field of composite microbial fertilizer manufacturing, and discloses a composite microbial fertilizer synergist capable of cooperating with multiple beneficial strains and a production method thereof, and the production method comprises the following steps: mixing a hydrophilic temporary storage agent with a basic carrier, spraying critical water lower than a microbial activation threshold value, and uniformly stirring; inducing the surface gelling of the hydrophilic temporary storage agent by using water to form a viscous micronucleus; the preparation method comprises the following steps: firstly, adding a hydrophobic separant and a core flora, realizing spontaneous selective coating by utilizing a micronucleus viscous surface, and finally dispersing a pioneer flora into a matrix outside a coating structure. A microcosmic functional structure of particles is preset in a preparation process, so that the activation relationship of microorganisms after meeting water is competed by disordered synchronous resources, and the microcosmic functional structure of the particles is changed into the microcosmic functional structure of the particles, and the microcosmic functional structure of the particles is changed into the microcosmic functional structure of the particles; the method is converted into deterministic time sequence ecological niche relay, so that the advantage of diversity of strains in the formula can be exerted in practical application.

The invention provides a composite conditioner for acid soil remediation and application thereof, and belongs to the technical field of agricultural environment and soil remediation. The composite conditioner comprises a first component, a second component and a third component which are independently packaged, wherein the modified straw biochar in the first component is used as a'microcarrier 'and a'buffer', calcium magnesium ore powder containing high-activity calcium magnesium oxide is loaded, slow release of alkaline substances is achieved, local over-alkalinity and hardening risks are avoided, and the porosity and the fertilizer retention capacity are improved; the second component is compounded with high-molecular polymer hydrolytic polymaleic anhydride, polyaspartic acid and polyglutamic acid according to a specific proportion, and the synergistic chelation capacity to aluminum and manganese ions and the dispersion capacity to soil particles are obviously superior to those of any single component and other proportion combinations; the third component selects an acid-resistant compound strain and is supplemented with amino acid to serve as a nutrient substance for rapid proliferation of the acid-resistant compound strain, so that high survival rate, colonization success rate and functional expression efficiency of the exogenous flora in an acidic stress are ensured.

The invention relates to the technical field of sewage treatment equipment, in particular to a three-phase flow short-range anaerobic ammonium oxidation and anaerobic ammonium oxidation flora construction system construction filler and a structure thereof.The three-phase flow short-range anaerobic ammonium oxidation and anaerobic ammonium oxidation flora construction system construction filler comprises a filler body, the filler body is made of a porous ceramsite material, a columnar cavity is formed in the center of the filler body, and through type communicating pores are evenly distributed in the side wall of the filler body; the filler realizes comprehensive improvement of mass transfer efficiency, biological activity and engineering applicability through collaborative design of a structure and a material: a three-dimensional flow field remarkably improves the mass transfer coefficient, the ammonia nitrogen removal rate is greatly improved, and the nitrite accumulation rate is kept at a relatively high level; and the nano coating enhances the nitrosobacteria adhesion ability, and effectively prolongs the blocking period in cooperation with rib wire microbubble scouring.

The current invention relates to a purified bacterial strain for improving plant growth and / or yield. The invention also relates to a bacterial population, a microbial active ingredient, an agriculturally active ingredient, a synthetic composition, and methods for improving plant growth and / or yield by improving a trait of agronomic importance in a plant and by conferring resistance to a plant pathogen infection.

The invention belongs to the technical field of compound microbial fertilizer manufacturing, and discloses a compound functional flora fertilizer formula for cadmium-contaminated soil remediation, which comprises the following steps: atomizing a premixed solution containing a second functional flora vitrification protective agent and an ionic cross-linking agent, applying the atomized premixed solution to a wet organic matrix preloaded with multivalent cations, and carrying out osmotic pressure driving to prepare the compound functional flora fertilizer for cadmium-contaminated soil remediation. According to the method, when a glassy state protection structure is formed around the flora in situ, a gel network is formed at an interface to anchor the flora, microcosmic protection and firm anchoring of the core functional flora are synchronously completed in the wet mixing process of the fertilizer, irreversible physical damage caused by a traditional drying process to the strain is avoided, and the method is suitable for large-scale industrial production. The bacterial strain with strong function and weak environmental tolerance can be applied to the product with high survival rate, and the life rhythm of orderly release of functional flora is formed.

This invention provides a method for producing single-cell protein using a mixed bacterial culture, comprising the following steps: performing primary seed culture on methanogenic bacteria, *Tricholoma materata*, and *Glucosinolate methyl-eating bacteria* to obtain primary seed solutions; mixing the primary seed culture solutions evenly to obtain a mixed bacterial solution; inoculating the obtained mixed bacterial solution into a secondary seed culture medium for cultivation to obtain a secondary seed solution; inoculating the obtained secondary seed solution into a fermentation medium for scale-up cultivation to obtain a fermentation broth; separating the solid and liquid components of the obtained fermentation broth, harvesting the bacterial cells, and obtaining single-cell protein. This method for producing single-cell protein using a mixed bacterial culture improves the utilization rate of the carbon source methane and the yield of single-cell protein.

Provided herein are pharmaceutical compositions comprising a bacterial population. The bacterial population comprises one bacterial strains or at least two bacterial strains. Such pharmaceutical compositions can be administered to a subject for prevention and / or treatment of dysbiosis and dysbiosis associated conditions and diseases, such as infant gastrointestinal diseases.