159 results about "Sequenceome" patented technology

According to the invention, a structural domain reaction substrate sequence consisting of Exon1 (E1), P1 and Exon2 (E2) sequences of ribozyme is mutated, and E1, P1 and E2 sequences are mutated under the condition of maintaining the structural stability, so that the Azoarcus group I intron ribozyme still has enzyme activity and can maintain the capability of forming circular RNA (Ribose Nucleic Acid). The invention discloses a flexible vector construction method for preparing circular RNA (Ribonucleic Acid) without limitation of a substrate sequence, which comprises the following steps: determining a target to-be-cyclized site sequence NNUNNNN, and segmenting the target to-be-cyclized site sequence NNUNNNN into E1: NNU and E2: NNNN; with E1 and E2 sequences as references, designing IGS sequences to respectively form complementary pairing with E1 and E2; and 5'and 3 'homologous arms, IRES, CDS and other elements are respectively added. The method provided by the invention can be used for preparing the circular RNA for any target sequence, has no residual sequence, and has relatively high cyclization efficiency. The FlexCirc cyclization system designed on the basis of Azoarcus group I intron ribozyme can form the circular RNA, the cyclization substrate sequence has the characteristic of flexible design, and the cyclization efficiency can realize a relatively high cyclization proportion.

The invention discloses a CRISPR / Cas12a-RPA (clustered regularly interspaced short palindromic repeats / CRISPR associated protein 12a-recombinase polymerase amplification)-based sequence combination for rapidly detecting mouse hepatitis virus and a kit thereof, and belongs to the technical field of biological detection The sequence combination comprises an RPA primer pair sequence, a crRNA sequence and an ssDNA probe sequence; the kit comprises an RPA primer pair sequence, a crRNA sequence and an ssDNA probe sequence, the kit further comprises a mouse hepatitis virus negative control standard substance, RNase-free water, an RPA reaction buffer solution, an NEB buffer solution, Lba Cas12a nuclease, dry powder RPA reaction microspheres containing recombinase polymerase and magnesium acetate with the concentration of 280 mM. The kit can be used for rapidly detecting the mouse hepatitis virus. The primer sequence combination provided by the invention is good in conservative property and specificity and relatively high in sensitivity, and rapid detection of the mouse hepatitis virus can be realized.

The invention discloses a self-assembly-based DNA molecular weight standard preparation method, which comprises: S1, chemically synthesizing two single-stranded DNAs having the same length but not completely complementary, and each of the two single-stranded DNAs is composed of two parts of sequences: one part is a complementary sequence, and the other part is a self-assembly complementary sequence; s2, mixing in a reaction buffer solution for directional self-assembly, and adding ligase; s3, reacting at room temperature for 0.5-10 minutes, regulating and controlling the number of self-assembly times by controlling the reaction time, and increasing the base number equal to the base number of the single-stranded DNA in each connection to form a plurality of DNA molecular weight standards with sequence lengths gradually increased in an equal difference manner; s4, carrying out thermal inactivation treatment; and S5, adding an electrophoresis loading buffer solution to prepare a DNA molecular weight standard, and storing at low temperature. The DNA molecular weight standard is rapidly prepared through the one-pot method, and the method has the advantages of being simple in process, low in manufacturing cost, high in expansibility and the like and has good application prospects.

PEPTIDES The peptide of the present invention presents 3 short peptide sequences which are spaced apart by a linker sequence and include either a specific N Terminal sequence (SEQ ID No: 1) or a specific C-Terminal sequence (SEQ ID No: 2), or both. The linker provides a consistent spacing between the short peptide sequences and structural integrity and together with the specified N-Terminal and / or C-Terminal sequences surprisingly improves solubility and film forming ability. The short peptide sequence can have a sequence which comprises or consists of any one of SEQ ID Nos: 3 to 26. The peptides are very soluble and are able to form films and gels, making them particularly attractive for personal care products.

The invention provides a method for eliminating ribosomal RNA in a mouse total RNA sample, which comprises the following steps: carrying out reverse transcription on the total RNA in the mouse sample into cDNA, and amplifying the cDNA by adopting a primer sequence group to obtain a DNA fragment covering all sequences of the mouse ribosomal RNA; carrying out amplification by taking the DNA fragment as a template and adopting a single primer in the primer sequence group to obtain a single-stranded DNA probe; hybridizing the total RNA of the mouse sample by adopting a single-stranded DNA probe, and performing RNase H digestion to obtain a digestion product; sequentially carrying out DNase I digestion and RNA purification on the digestion product to obtain a target product; wherein the primer sequence group comprises upstream primers from the first to the nineteenth and downstream primers from the first to the nineteenth, and the primer sequence group has the characteristics of low cost, stable performance and high reduction efficiency.

The invention belongs to the field of canine distemper virus detection, and particularly relates to a canine distemper virus RT-RAA CRISPR / Cas 12b detection kit and a detection method thereof. According to the kit, isothermal amplification is performed by using an RT-RAA universal primer, and then detection is performed by using a CRISPR / Cas 12b detection system consisting of target sequence sgRNA, so that amplification of a target fragment of the N gene of the canine distemper virus and target detection visualization can be effectively realized. The result of the embodiment shows that the RT-RAA CRISPR / Cas 12b detection kit finally designed by the invention is high in specificity and does not have cross reaction with other viruses, the lowest visual detection limit reaches 4.77 * 10 < 1 > copies / mu L, the virus can be instantly detected when the RT-RAA CRISPR / Cas 12b detection kit is used for detecting the CDV, the labor and equipment cost is low, the period is short, and the detection time does not exceed 2 hours. The rapid detection technology can be popularized and applied to epidemiological investigation and epidemic situation monitoring of the canine distemper virus, is suitable for epidemic disease monitoring of wild animals, quarantine of zoos and on-site rapid detection of clinical samples of canines, and has good practical significance and wide market prospects.

The invention relates to the technical field of biology, and particularly discloses a kit for detecting haemophilus influenzae based on PCR (Polymerase Chain Reaction) / RAA-CRISPR-Cas13a. The invention discloses a kit for detecting a haemophilus influenzae gene on the basis of PCR (Polymerase Chain Reaction) / RAA-CRISPR-Cas13a. The kit comprises a primer pair for specifically amplifying haemophilus influenzae genes and crRNA, the sequence of the crRNA is composed of an anchoring sequence used for being combined with Cas13a protein and a guide sequence of a target sequence of the haemophilus influenzae genes, and the guide sequence is shown as the 39-66th site of SEQ ID No.28. The invention constructs a method for detecting haemophilus influenzae genes by combining a PCR or RAA technology with a CRISPR-Cas13a technology, the method is simple, convenient and rapid, has higher sensitivity and specificity, can reach a single copy level in sensitivity, and can be used for clinically and rapidly identifying whether the haemophilus influenzae is infected or not.

Provided are a method for constructing a soluble expression plasmid mutant library, and the use thereof. The method for constructing the soluble expression plasmid mutant library comprises: introducing mutations into a ribosome binding site on a soluble expression plasmid, a sequence composition and length of a region between the ribosome binding site and an initiation codon of a fusion protein to be expressed, and a translation initiation region, so as to obtain a mutant library. By means of the method, plasmid mutants with increased expression levels are obtained, thereby increasing the expression level of a target fusion protein and the final yield of a target polypeptide. The present invention solves the problem of low soluble expression levels of certain polypeptides in Escherichia coli.

According to the context-understanding-oriented generative reading understanding method and device and the medium, vector representation of a to-be-read understanding text is obtained by using an encoder of a pre-training language model, a pipeline decoder is used during decoding, an understanding result is generated for the vector representation in a parallel decoding mode, the pipeline decoder is a stacked Transformer decoder, and the pipeline decoder is used for decoding the to-be-read understanding text. And starting to generate a new sub-sequence every delay time delta t, generating the first lexical element of the sub-sequence by depending on the lexical element generated by the previous sub-sequence until the maximum sub-sequence number is reached or all the previous sub-sequences are subjected to lexical element generation, and forming a final understanding result by the sub-sequences obtained by decoding. The pipeline decoder has good performance in reading and understanding of phrase-level and sentence-level data sets, and compared with a sequence decoder, the pipeline decoder has higher reasoning speed and lower GPU (Graphics Processing Unit) memory usage amount, and the generation quality can meet the requirement.

Provided are compositions and methods for selectively reducing the amount of antibiotic resistant and / or virulent bacteria in a mixed bacteria population, or for reducing any other type of unwanted bacteria in a mixed bacteria population. The compositions and methods involve targeting bacteria that are differentiated from other members of the population by at least one unique clustered regularly interspaced short palindromic repeats (CRISPR) targeted DNA sequence. The compositions and methods can be readily adapted to target any bacteria or any bacteria plasmid, or both.

A process for the manufacture of glycosylated p75NTR neurotrophin binding protein (NBP)-Fc fusion protein, comprising: a p75NTR(NBP) portion, having at least 85% sequence identitywith Seq ID No. 2; and an immunoglobulin Fc portion. wherein, the p75NTR(NBP) and Fc portions are connected via a linker, the linker comprises a peptide of formula Gx, where x is 1, 2, 3, 4, 5 or 6 wherein the linker does not comprise or consist of the sequence GGGGS, characterised by expression from CHO cells, wherein cells are harvested within earlier of 30 hours of cell culture viability reaching 75% or, 12 days post inoculation.

Disclosed herein are engineered LSR sequences, compositions, and uses including in genome-editing systems and therapeutic compositions. The LSRs can facilitate nucleic acid recombination at particular attachment sites, using suitable donor sequences. The disclosed LSRs may be used to site-specifically recombine or integrate nucleic acid sequences for a variety of purposes, including treatment of human diseases.

The invention discloses an LAMP (loop-mediated isothermal amplification) detection primer group for flavobacterium psychrophilum and application of the LAMP detection primer group, and belongs to the technical field of molecular biology. The method comprises the following steps: firstly, searching a specific sequence which is completely not matched with other genomes according to a whole genome sequence of flavobacterium psychrophilum, and designing a specific LAMP detection primer group according to the specific sequence; the LAMP detection primer group comprises a pair of outer primers and a pair of inner primers, and the sequence compositions of the outer primers and the inner primers are respectively shown as SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3 and SEQ ID NO: 4. The primer group is used for constant-temperature fluorescent LAMP detection of flavobacterium psychrophilum, and the result shows that the LAMP detection primer group designed by the invention has a good amplification effect on detection of target flavobacterium psychrophilum, and has the advantages of strong specificity, high sensitivity, convenience and rapidness in operation and the like when being used for detecting flavobacterium psychrophilum. The method is suitable for early screening, accurate diagnosis and epidemic situation detection of freshwater fish bacterial diseases caused by flavobacterium psychrophilum.

The invention belongs to the technical field of biological detection, and discloses a sequence combination for rapidly detecting influenza A based on CRISPR / CAS12a-MIRA and application, the sequence combination comprises an MIRA primer pair (SEQ ID NO: 1-2) of a targeted influenza A virus M gene highly conserved region (M1 fragment), and CRISPR / Cas12a detection probes (crRNA SEQ ID NO: 3 and phosphorothioate modified FAM-biotin probe SEQ ID NO: 4). By optimizing a buffer system (containing PEG 8000 and betaine), MIRA amplification and CRISPR detection are continuously completed in a closed tube at 37-42 DEG C, and a result is output within 30 minutes. The detection sensitivity of the kit reaches 50 copies / [mu] L, the specificity is 100%, and subtypes such as H1N1, H3N2, H5N1 and H7N9 can be covered. Compared with the prior art, according to the scheme, the detection time is shortened by 67%, the probe cost is reduced by 80%, and high anti-interference performance (tolerance to 10% mucoprotein and 5% whole blood) is achieved. The kit is suitable for on-site instant detection of saliva, nasopharynx swabs and environmental samples, and an efficient and portable solution is provided for basic medical institutions and prevention and control of sudden epidemic situations.

The invention provides a quality control kit of a nucleic acid detection system as well as a quality control method and application of the quality control kit. The quality control kit comprises the coliphage MS2 and a first sequence group. The first sequence group comprises a forward primer of a sequence as shown in SEQ IQ NO: 1, a reverse primer of a sequence as shown in SEQ IQ NO: 2 and a probe of a sequence as shown in SEQ IQ NO: 3. The quality control method comprises the following steps: mixing a specimen with bacteriophage MS2, and carrying out nucleic acid extraction to obtain an eluent; and mixing an eluent with the feline blood plasma bacterial plastid, the first sequence group and the second sequence group, and carrying out multiple real-time polymerase chain reaction. The quality control kit of the nucleic acid detection system is used for evaluating the recovery rate of nucleic acid and the whole-process quality control of the nucleic acid detection system.

The application provides a nucleic acid sequence and a method for detecting biomolecules based on a self-driven hybrid chain reaction (SHCR), which comprises a priming chain AI, a blocking chain B, a hairpin probe H1, a hairpin probe H2, a hairpin probe H3 and a hairpin probe H4, the priming chain AI comprises an aptamer sequence of a to-be-detected biomolecule and a starting sequence I, the sequence of the starting sequence I is a*‑b*, wherein a* and b* are gene fragments; the 5' end of the hairpin probe H1 is connected with b*, and the 3' end of the hairpin probe H3 is connected with a*. The application further provides a kit for detecting biomolecules based on the SHCR and a method for detecting biomolecules, in particular, a method for detecting a kanamycin residual amount in food. Through ingenious design, when the to-be-detected biomolecule exists, the sequence combination of the above biomolecule can initiate the self-driven hybrid chain reaction, the priming chain can be autonomously supplemented, the self-driven reaction process is reversely activated, thereby a more significant FRET signal is generated, and the detection sensitivity is improved.

The application discloses a sequence construction method and system. The method constructs a switching matrix based on the number of various types of elements in the sequence, establishes a unified constraint system, and obtains a numerical solution of the switching matrix through optimization. Based on the numerical solution, a graph structure is constructed and connectivity is determined to determine whether a complete sequence can be formed; when the connectivity is satisfied, a target sequence that meets the switching relationship and quantity requirements is generated based on the graph structure. The method supports segment-level structure expression, modeling of the head-tail relationship of the augmented matrix, and sub-sequence combination expression, and is suitable for multiple sequence construction tasks. Accordingly, the application also provides a sequence construction system, which includes a matrix modeling module, a constraint construction module, a solving module, a connectivity determination module, and a sequence generation module.

The invention relates to the technical field of nucleic acid detection, and particularly provides a CRISPR-Cas12a cascade signal amplification system and application thereof. The system comprises a first-stage sensing unit, a second-stage sensing unit and a third-stage sensing unit, wherein the first-stage sensing unit is configured to recognize a target to be detected and activate the trans-cleavage activity of first-stage Cas12a protein; an engineered scaffold RNA composed of an RNA sequence and a DNA sequence is cut, the obtained RNA sequence serves as an activator of a second-stage Cas12a protein, and the second-stage Cas12a protein obtains trans-cleavage activity, so that a fluorescence report probe is cut, and a signal is output. According to the system, an activation event of a first Cas12a is converted into an exponential signal output unit of a second Cas12a, and a cascade amplification network is formed. According to the design, background signal leakage is eradicated from a physical mechanism, and the detection sensitivity is remarkably improved. The detection method provided by the invention is low in cost, high in sensitivity and high in reaction speed.

The application provides a data expansion method and device, the method comprises the following steps: obtaining N groups of real source data, each group of real source data contains a data sequence; dividing the data sequences in the N groups of real source data into a plurality of sequence combinations, each sequence combination is a calculation group; performing weighted calculation on each calculation group according to the sequence number requirement of weighted average calculation, to obtain the weight value allocated to each calculation group; performing weighted average on the data sequences in each calculation group according to the weight value allocated to each calculation group, to obtain the data expansion sequence of each calculation group. In the data expansion method, the calculation results of all calculation groups are not all considered all data sequences, therefore, in the case that some part of data sequences are abnormal, more calculation results are not affected because they do not involve abnormal data sequences, which greatly avoids the influence of abnormal sequences in small data on the final data expansion sequence.

Split-pegRNA recorder reagents and methods are provided that provide specific molecular interactions within cells induce precise genome editing that inserts an event-specific barcode sequence into the predetermined genomic locus of DNA Tape. The split pegRNAs include (a) a crRNA component, including in 5′ to 3′ order (i) a spacer sequence for a genomic locus of interest; (ii) a crRNA repeat sequence necessary for binding to a CRISPR-Cas protein; and (iii) a first RNA extension; and (b) a petracrRNA component, including in 5′ to 3′ order (i) a second RNA extension; (ii) a petracrRNA antirepeat sequence necessary for binding to a CRISPR-Cas protein; (iii) a gRNA scaffold; wherein the crRNA component and the petracrRNA component are not covalently bound to each other.

Provided are methods for producing polysaccharides in bacteria by expressing in a bacterium one or more coding sequences selected from the group consisting of a pglF dehydrogenase coding sequence, a wbpP UDP-N-acetyl-d-glucosamine C4 epimerase coding sequence, a wcfR aminotransferase coding sequence, and a wcfS phospho-glycosyltransferase coding sequence, a wcfQ glycosyltransferase coding sequence, a wcfO pyruvyltransferase coding sequence, a wcfP glycosyltransferase coding sequence, a wcfM UDP-galactopyranose mutase coding sequence, a wcfN glycosyltransferase coding sequence, a wza polysaccharide export protein coding sequence, a wzx fippase coding sequence, a wzy polymerase coding sequence, and a wzz coding sequence, wherein at least one of the coding sequences is heterologous to the bacterium. Also provided are expression cassettes with one or more of the disclosed coding sequences, recombinant bacteria that harbor one or more of the expression cassettes, and methods for producing immunogenic compositions using the polysaccharides produced by the recombinant bacteria.

Embodiments of the present disclosure provide a data processing method and apparatus, a storage medium and an electronic apparatus. The method includes: dividing data to be transmitted into G groups, wherein each group respectively includes D(g) subgroups, each of the subgroups includes one or more pieces of data, g is an identifier corresponding to each group, g=1, 2, . . . , G, and D(g)>=1; respectively performing first processing on the D(g) subgroups in each group to obtain D(g) groups of first data sequences; performing second processing on the D(g) groups of first data sequences in each group to obtain a second data sequence of each group; and transmitting G groups of the second data sequences. By means of some embodiments of the present disclosure, the problem of transmission interference in the related art can be solved.

Various aspects of the present disclosure generally relate to wireless communication. In some aspects, a user equipment (UE) may receive a probabilistic shaping configuration including a configuration for peeling-based arithmetic coding. The UE may transmit an uplink communication in accordance with the probabilistic shaping configuration, wherein the configuration for peeling-based arithmetic coding includes a configuration for determining a sequence composition during a composition element selection stage and a configuration for generating a sequence during a sequence generation stage, wherein the configuration for generating the sequence during the sequence generation stage includes a configuration for generating the sequence in accordance with the sequence composition determined during the composition element selection stage. Numerous other aspects are described.

The present invention refers to a sgRNA molecule comprising a targeting domain for specifically targeting a SNP in the BEST1 coding region of a pathologic allele, wherein said targeting domain consists of a sequence selected from the group consisting of SEQ ID NO: 3-8, 41-44, 14-20, 50-52 and 54-55, or a sgRNA molecule combination of specifically defined first and second sgRNA molecules, wherein the first and the second sgRNA molecule each comprise a targeting domain for specifically targeting a SNP in the BEST1 gene coding or non-coding region of a pathologic allele. The present invention also refers to a nucleic acid comprising a sequence that encodes the sgRNA molecule or sgRNA molecule combination and to nucleic acid combinations. The present invention further relates to a recombinant adenovirus-associated virus (AAV) comprising the nucleic acids according to the present invention or recombinant AAV combinations. The sgRNA molecule, the nucleic acid, the recombinant AAV and combinations are useful tools for editing of the target domain in the bestrophin-1 (BEST1) gene to restore BEST1 channel function by e.g. CRISPR / Cas9)-based gene editing. The present invention further relates to the sgRNA molecule, the sgRNA molecule combination, the nucleic acid, the nucleic acid combination, the recombinant AAV and the recombinant AAV combination for use in a method for treatment of the human or animal body by surgery or therapy and for use in method of treating or preventing BEST1-related retinopathies, in particular autosomal dominant BEST1-related retinopathies.

The embodiment of the present invention relates to the technical field of position detection, and discloses a workpiece detection method based on PLC, including: performing a length division operation on the length information of the conveyor belt in the workpiece transportation device at a set length interval to obtain a corresponding conveying sequence group; using a set storage area in the data storage area of the PLC as a position storage area, and a data position sequence group is set in the position storage area; when receiving that the workpiece sensor detects the workpiece to be processed, determining the current position information of the corresponding workpiece, and performing a real-time update operation on the status information of each position data block in the data position sequence group according to the current position information. In the workpiece detection method based on PLC in the embodiment of the present invention, each position data block in the data position sequence group corresponds one by one to the length marking block in the conveying sequence group, thereby realizing the accurate mapping of the workpiece position.

This application provides a data transmission method and an apparatus. In an example method, a first sequence and a common sequence group are obtained, where a quantity of sequences in the common sequence group is N; the first sequence includes K first sub-sequences, lengths of the K first sub-sequences are sequentially B1, B2, . . . , BK, K is a positive integer, B1=log2N, and the length B1 of a 1st first sub-sequence is greater than or equal to a length of another first sub-sequence. The K first sub-sequences are separately mapped into K second sub-sequences based on the common sequence group. A second sequence including the K second sub-sequences is output.

The application discloses a test strip RPA primer for detecting potato brown stem nematode, wherein the primer is composed of a nucleotide sequence shown in SEQ ID No:1 and SEQ ID No:2; a combination of the primer and a probe is also disclosed, wherein the probe is composed of a sequence shown in SEQ ID No:3. In addition, a detection kit comprising the primer and the probe combination is disclosed. The test strip RPA primer and the probe combination of the application have the advantages of high specificity and sensitivity for detecting potato brown stem nematode, low requirement for equipment and personnel quality, simple operation, fast detection speed and low cost, and are suitable for port quarantine and primary site detection.

The invention provides a polypeptide composed of a sequence SEQ ID NO: 1 or a variant of the polypeptide, the sequence of the variant has at least 85% identity with the sequence SEQ ID NO: 1, and the polypeptide is used for prolonging the life of a subject. The invention also provides a nucleic acid sequence for coding the polypeptide, a gene construct containing the nucleic acid sequence or an expression vector containing the gene construct, which are used for the application. The polypeptides, nucleic acid sequences, gene constructs or expression vectors of the invention may be administered in the form of a pharmaceutical composition with at least one pharmaceutically acceptable excipient, diluent or carrier.

Methods, kits, and oligonucleotides used in the detection of coronavirus, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), are disclosed. In some aspects, the oligonucleotides are primers or probes used in the described methods or kits. The nucleotide sequence of the oligonucleotide consists of 300 or less, 150 or less, or 40 or less continuous nucleotides from a nucleotide sequence selected from the group consisting of: SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, and SEQ ID NO: 5, or is a variant thereof. In some embodiments, the oligonucleotide is modified with an internal spacer or a detectable label. For example, the 5′ terminus is labeled with a fluorophore and the 3′ terminus is complexed to a quencher of fluorescence of said fluorophore. In some embodiments, the nucleotide sequence of the oligonucleotide further comprises a universal tail sequence.