193 results about "Sequenceome" patented technology

The invention relates to the molecular medicine and gene treating field, in particularly, a gene segment which is new, cell target special, and can eliminate replication ability and synthesizing ability of nucleocapsid protein from source and clean the pollution protein. The gene segment is composed of a recombinant aden-associated virus vector helper plasmid non-structure and/or structure gene, and at least one copy cell special microRNA target sequence which can be used in multiple cell strains directly, and also can be used for constructing recombinant aden-associated virus plasmid and vector to prepare medicine, basic medicine and clinic gene treating.

The invention provides a polypeptide chip, which comprises a substrate and n polypeptides distributed on the substrate in an array, each polypeptide sequence has 10-20 amino acids, a group consistingof sequences of first to nth polypeptides covers at least 95% of a viral protein sequence, the adjacent polypeptides have an overlap of 3-8 amino acids, and n is 810-1370. According to the method, proteomics and system biology strategies are adopted, all coded protein sequences of COVID-19 are extracted from NCBI data, an SARS-CoV-2 virus proteome polypeptide chip is designed and prepared, and panoramic scanning of all SARS-CoV-2 virus antibodies in blood of a novel pneumonia virus infected patient is achieved.

The invention discloses a distribution method of a signature sequence or a signature sequence group on an E-HICH, comprising the following steps of determining a value of each parameter needed when distributing the signature sequence or the signature sequence group to each user equipment UE which shares a same enhanced physical uplink channel (E-PUCH); and distributing corresponding signature sequence or signature sequence group to each UE which shares the same E-PUCH according to the value of each parameter. By the method, different signature sequences or different signature sequence groupsare distributed to each UE sharing the same E-PUCH, and therefore, NODEB can respectively feed back control information of the E-PUCH of each UE to each UE sharing the same E-PUCH by distributing thesignature sequence or the signature sequence group to each UE.

The invention discloses a primer set and method for identifying variety authentication and seed purity of tomatoes. Sequences of 48 pairs of primers of the primer set for identifying variety authentication of tomatoes and purity of seeds is successively as shown in sequences 1-96 in a sequence table, a sequence 2n and a sequence 2n-1 form a primer pair, and n is a natural number in 1-24. The method for identifying variety authentication and seed purity of tomatoes comprises the following steps: carrying out PCR amplification on DNA of tomatoes to be detected by using the primer set; and determining variety authentication and seed purity of the tomatoes to be detected according to difference of PCR products of the tomatoes to be detected. Experiments prove that the primer set not only can be used for identifying the variety authentication of the tomatoes, but also can be used for identifying the seed purity of the tomatoes, and has the advantages that the accuracy is high, results are stable, the primer set is not affected by environment conditions and development stages, statistics is simple and convenient and the like.

The invention discloses the use of variovoraxboronicumulans CGMCC 4969 in bioconversion of 3-cyanopyridine for forming nicotinamide. The nitrile hydratase gene cluster produced by the strain consists of a DNA sequence represented by SEQ ID No.1 in a sequence table; the DNA consisting of the sequence represented by SEQ ID No.1 is recombined onto a pET28a plasmid and can be induced to express in EscherichiacoliBL21(DE3) strain; and the Escherichia coli cells containing expressed proteins and cell extracting solution can convert 3-cyanopyridine into nicotinamide.

The invention relates to a CRISPR (clustered regularly interspaced short palindromic repeat) detection primer group for mycoplasma pneumoniae and an application of the CRISPR detection primer group, and belongs to the technical field of gene detection of a CRISPR technology. The primer group comprises an amplification primer pair and crRNA (CRISPR-derived ribonucleic acid), wherein the amplification primer pair is used for amplifying a sequence shown as SEQ ID NO. (sequence identifier number) 1 of the mycoplasma pneumoniae; crRNA comprises an anchor sequence and a guide sequence; the anchor sequence specifically identifies a Cas protein; and the guide sequence is matched with a target sequence segment in the sequence of SEQ ID NO. 1. The primer group detects the mycoplasma pneumoniae through the CRISPR technology; the detection time of the mycoplasma pneumoniae is shortened; and the detection can be accomplished within 60min. A specific sequence combination obtained by screening acts as the primer group for detection, the primer group has the advantages of high sensitivity and strong specificity, and a limit of detection of the primer group can reach 30 copies. The primer group isused for the CRISPR detection of the mycoplasma pneumoniae, is independent of a complicated variable temperature amplification instrument such as a qPCR (quantitative polymerase chain reaction) instrument, and a CRISPR-Cas technology has wide application prospects in instant diagnosis of the mycoplasma pneumoniae.

The invention discloses a small interfering RNA (siRNA) inhibiting expression of a myostatin (MSTN) gene in chicken and application thereof. The invention provides an siRNA which acts on the MSTN gene, wherein the siRNA is a) a double-stranded RNA composed of the sequence 1 and the sequence 2 in a sequence table, b) a double-stranded RNA composed of the sequence 3 and the sequence 4 in the sequence table or c) a double-stranded RNA composed of the sequence 5 and the sequence 6 in the sequence table. The invention obtains the siRNA effectively inhibiting expression of the MSTN gene in chicken,real-time quantitative polymerase chain reaction (PCR) verifies that the siRNA effectively reduces expression of MSTN in molecular biology, and chicken with skeletal muscles obviously increased are successively obtained through microinjection test in embryology. The above results show that the siRNA provided by the invention can be used for breeding chicken, thus improving the meat yields of broilers, especially high quality chicken.

The invention discloses whole genome re-sequencing analysis and a method for the whole genome re-sequencing analysis. The method for whole genome re-sequencing analysis comprises the following steps of: acquiring a plurality of sequencing sequences obtained by identifying a DNA sequence of a sample to be detected, dividing the plurality of sequencing sequences into a plurality of sequencing sequence groups, based on each sequencing sequence group, executing the following operations in parallel: sequentially or parallelly comparing each sequencing sequence in the sequencing sequence group witha reference genome, and determining a corresponding position of each sequencing sequence on the reference genome and a corresponding chromosome number, and according to the corresponding position of each sequencing sequence on the reference genome and the corresponding chromosome number, conducting sequencing and duplicate removal on each sequencing sequence, and generating a sequencing sequence library corresponding to each chromosome.

The invention discloses Variovorax boronicumulans CGMCC 4969 capable of biologically degrading acrylamide. The amidase gene of the Variovorax boronicumulans CGMCC 4969 is composed of a DNA sequence represented by SEQ ID No:1 in a sequence table; DNA fragments composed of the sequence represented by the SEQ ID No:1 are inserted into an Escherichia coli expression vector pET28a plasmid to obtain a recombinant pET28a-amidase; and the recombinant pET28a-amidase is transformed into an Escherichia coli Rosetta strain, the amidase is expressed through induction, and Escherichia coli cells containing expression proteins can degrade acrylamide.

The invention discloses a protein for controlling rice lesion mimic characters. The protein is formed by an amino acid sequence as shown by SEQ ID NO. 2 in a sequence table or a derivative sequence thereof. The invention further discloses a gene for encoding the protein. The gene is formed by a nucleotide sequence as show by SEQ ID NO. 1 in the sequence table. The lesion mimic gene provided by the invention has a good effect on resistance to rice bacterial blight, can be used for improving the bacterial blight resistance of nonglutinous rice, and provides a new way for breeding the nonglutinous rice capable of resisting bacterial blight; secondly, a new gene knockout technology is applied, a lesion mimic material under the background of japonica rice is acquired successfully, and a new method for utilizing the gene to culture japonica rice variety capable of resisting bacterial blight is provided. In addition, the gene provided by the invention is controlled by a single recessive gene, a transgene or filial generation can be simply identified and selected, and the convenience in application is realized.

A mutant, non-naturally occurring or transgenic plant cell comprising: (i) a polynucleotide comprising, consisting or consisting essentially of a sequence encoding a neoxanthin synthase and having at least 60% sequence identity to SEQ ID NO:1 or SEQ ID No. 6; (ii) a polypeptide encoded by the polynucleotide set forth in (i); (iii) a polypeptide having at least 66% sequence identity to SEQ ID NO:2 or at least 60% sequence identity to SEQ ID No. 7; or (iv) a construct, vector or expression vector comprising the isolated polynucleotide set forth in (i), and wherein the expression or activity of the neoxanthin synthase is modulated as compared to a control or wild type plant.

The invention provides a lipase mutant with high esterification activity and particularly provides a polypeptide. The polypeptide is selected from (1) a polypeptide with an amino acid sequence represented by SEQ ID NO:7 and (2) a polypeptide formed by the sequence represented by SEQ ID NO:7 and a sequence for promoting the expression and purification of the sequence represented by SEQ ID NO:7. Theinvention further provides a polynucleotide sequence encoding the polypeptide, a corresponding nucleic acid construct, a host cell and application of the polypeptide, the polynucleotide sequence, thenucleic acid construct and the host cell in the preparation of biodiesel.

The invention relates to hot start Taq DNA polymerase preparation method. The method includes: screening a positive clone bacterium from an artificial synthesis ssDNA library by means of SELEX (systematic evolution of ligands by exponential enrichment), wherein the positive clone bacterium is high in Taq DNA polymerase compatibility and stable in compatibility at a temperature of 45 DEG C or below; further analyzing a sequence of the clone bacterium; artificially synthesizing an ssDNA aptamer, and proportionally dissolving the aptamer and the Taq DNA polymerase in Enhancer solution. The hot start Taq DNA polymerase preparation method has advantages that in a PCR (polymerase chain reaction) process, hot start is realized at the first step as well as follow-up steps of PCR reaction, and accordingly non-specific amplification and primer dimers are avoided, and reaction specificity and sensitivity are improved.