188 results about "Sequenceome" patented technology

The invention discloses a primer pair and a method for identifying a new Oudemansiella oosporoides strain HCS, and belongs to the technical field of specific molecular markers, the preservation number of the new Oudemansiella oosporoides strain HCS is CGMCC NO.41593, and the sequences of the primer pair Y6-F1 / Y6-R2 are as shown in SEQ ID NO.2 and SEQ ID NO.3. The specific method comprises the following steps: extracting DNA of an Oudemansiella oosporoides strain to be detected; carrying out PCR (Polymerase Chain Reaction) amplification by taking the extracted DNA of the sample to be detected as an amplification template and a molecular specific labeled primer consisting of the sequence shown by the primer as an amplification primer; and carrying out agarose gel electrophoresis detection on the PCR product. The primer pair has the advantages that the primer pair can be used for quickly identifying the strain HCS, the operation is simple and convenient, the specificity is strong, the accuracy is high, and a technical basis is provided for protecting a new variety of the strain HCS.

According to the invention, a structural domain reaction substrate sequence consisting of Exon1 (E1), P1 and Exon2 (E2) sequences of ribozyme is mutated, and E1, P1 and E2 sequences are mutated under the condition of maintaining the structural stability, so that the Azoarcus group I intron ribozyme still has enzyme activity and can maintain the capability of forming circular RNA (Ribose Nucleic Acid). The invention discloses a flexible vector construction method for preparing circular RNA (Ribonucleic Acid) without limitation of a substrate sequence, which comprises the following steps: determining a target to-be-cyclized site sequence NNUNNNN, and segmenting the target to-be-cyclized site sequence NNUNNNN into E1: NNU and E2: NNNN; with E1 and E2 sequences as references, designing IGS sequences to respectively form complementary pairing with E1 and E2; and 5'and 3 'homologous arms, IRES, CDS and other elements are respectively added. The method provided by the invention can be used for preparing the circular RNA for any target sequence, has no residual sequence, and has relatively high cyclization efficiency. The FlexCirc cyclization system designed on the basis of Azoarcus group I intron ribozyme can form the circular RNA, the cyclization substrate sequence has the characteristic of flexible design, and the cyclization efficiency can realize a relatively high cyclization proportion.

The invention discloses a correction method of PCR (Polymerase Chain Reaction) preference, a DNA (Deoxyribose Nucleic Acid) artificially synthesized sequence combination of a TCR-beta immune repertoire, a primer combination for amplifying the TCR-beta immune repertoire, a quantitative analysis method of the TCR-beta immune repertoire of a gDNA sample, a detection kit and application of the detection kit. The PCR preference correction method can effectively correct the preference generated in the PCR amplification process, the DNA artificially synthesized sequence combination can effectively adjust the primer concentration ratio of the multiple PCR primer combination, and the primer combination can efficiently and quantitatively amplify a TCR-beta immune repertoire without mutual interference as much as possible. According to the method, the immune repertoire can be accurately and quantitatively analyzed at the DNA level.

The invention discloses a CRISPR / Cas12a-RPA (clustered regularly interspaced short palindromic repeats / CRISPR associated protein 12a-recombinase polymerase amplification)-based sequence combination for rapidly detecting mouse hepatitis virus and a kit thereof, and belongs to the technical field of biological detection The sequence combination comprises an RPA primer pair sequence, a crRNA sequence and an ssDNA probe sequence; the kit comprises an RPA primer pair sequence, a crRNA sequence and an ssDNA probe sequence, the kit further comprises a mouse hepatitis virus negative control standard substance, RNase-free water, an RPA reaction buffer solution, an NEB buffer solution, Lba Cas12a nuclease, dry powder RPA reaction microspheres containing recombinase polymerase and magnesium acetate with the concentration of 280 mM. The kit can be used for rapidly detecting the mouse hepatitis virus. The primer sequence combination provided by the invention is good in conservative property and specificity and relatively high in sensitivity, and rapid detection of the mouse hepatitis virus can be realized.

The invention discloses a self-assembly-based DNA molecular weight standard preparation method, which comprises: S1, chemically synthesizing two single-stranded DNAs having the same length but not completely complementary, and each of the two single-stranded DNAs is composed of two parts of sequences: one part is a complementary sequence, and the other part is a self-assembly complementary sequence; s2, mixing in a reaction buffer solution for directional self-assembly, and adding ligase; s3, reacting at room temperature for 0.5-10 minutes, regulating and controlling the number of self-assembly times by controlling the reaction time, and increasing the base number equal to the base number of the single-stranded DNA in each connection to form a plurality of DNA molecular weight standards with sequence lengths gradually increased in an equal difference manner; s4, carrying out thermal inactivation treatment; and S5, adding an electrophoresis loading buffer solution to prepare a DNA molecular weight standard, and storing at low temperature. The DNA molecular weight standard is rapidly prepared through the one-pot method, and the method has the advantages of being simple in process, low in manufacturing cost, high in expansibility and the like and has good application prospects.

Provided are compositions and methods for selectively reducing the amount of antibiotic resistant and / or virulent bacteria in a mixed bacteria population, or for reducing any other type of unwanted bacteria in a mixed bacteria population. The compositions and methods involve targeting bacteria that are differentiated from other members of the population by at least one unique clustered regularly interspaced short palindromic repeats (CRISPR) targeted DNA sequence. The compositions and methods can be readily adapted to target any bacteria or any bacteria plasmid, or both.

PEPTIDES The peptide of the present invention presents 3 short peptide sequences which are spaced apart by a linker sequence and include either a specific N Terminal sequence (SEQ ID No: 1) or a specific C-Terminal sequence (SEQ ID No: 2), or both. The linker provides a consistent spacing between the short peptide sequences and structural integrity and together with the specified N-Terminal and / or C-Terminal sequences surprisingly improves solubility and film forming ability. The short peptide sequence can have a sequence which comprises or consists of any one of SEQ ID Nos: 3 to 26. The peptides are very soluble and are able to form films and gels, making them particularly attractive for personal care products.

The invention provides a method for eliminating ribosomal RNA in a mouse total RNA sample, which comprises the following steps: carrying out reverse transcription on the total RNA in the mouse sample into cDNA, and amplifying the cDNA by adopting a primer sequence group to obtain a DNA fragment covering all sequences of the mouse ribosomal RNA; carrying out amplification by taking the DNA fragment as a template and adopting a single primer in the primer sequence group to obtain a single-stranded DNA probe; hybridizing the total RNA of the mouse sample by adopting a single-stranded DNA probe, and performing RNase H digestion to obtain a digestion product; sequentially carrying out DNase I digestion and RNA purification on the digestion product to obtain a target product; wherein the primer sequence group comprises upstream primers from the first to the nineteenth and downstream primers from the first to the nineteenth, and the primer sequence group has the characteristics of low cost, stable performance and high reduction efficiency.

The invention belongs to the field of canine distemper virus detection, and particularly relates to a canine distemper virus RT-RAA CRISPR / Cas 12b detection kit and a detection method thereof. According to the kit, isothermal amplification is performed by using an RT-RAA universal primer, and then detection is performed by using a CRISPR / Cas 12b detection system consisting of target sequence sgRNA, so that amplification of a target fragment of the N gene of the canine distemper virus and target detection visualization can be effectively realized. The result of the embodiment shows that the RT-RAA CRISPR / Cas 12b detection kit finally designed by the invention is high in specificity and does not have cross reaction with other viruses, the lowest visual detection limit reaches 4.77 * 10 < 1 > copies / mu L, the virus can be instantly detected when the RT-RAA CRISPR / Cas 12b detection kit is used for detecting the CDV, the labor and equipment cost is low, the period is short, and the detection time does not exceed 2 hours. The rapid detection technology can be popularized and applied to epidemiological investigation and epidemic situation monitoring of the canine distemper virus, is suitable for epidemic disease monitoring of wild animals, quarantine of zoos and on-site rapid detection of clinical samples of canines, and has good practical significance and wide market prospects.

The invention relates to the technical field of biology, and particularly discloses a kit for detecting haemophilus influenzae based on PCR (Polymerase Chain Reaction) / RAA-CRISPR-Cas13a. The invention discloses a kit for detecting a haemophilus influenzae gene on the basis of PCR (Polymerase Chain Reaction) / RAA-CRISPR-Cas13a. The kit comprises a primer pair for specifically amplifying haemophilus influenzae genes and crRNA, the sequence of the crRNA is composed of an anchoring sequence used for being combined with Cas13a protein and a guide sequence of a target sequence of the haemophilus influenzae genes, and the guide sequence is shown as the 39-66th site of SEQ ID No.28. The invention constructs a method for detecting haemophilus influenzae genes by combining a PCR or RAA technology with a CRISPR-Cas13a technology, the method is simple, convenient and rapid, has higher sensitivity and specificity, can reach a single copy level in sensitivity, and can be used for clinically and rapidly identifying whether the haemophilus influenzae is infected or not.

The invention discloses an autism diagnosis method based on ARLSTM-GCNs, and relates to a medical mode recognition diagnosis method.The autism spectrum disorder diagnosis method based on an improved AR-LSTM and a fused attention graph convolutional network-ARLSTM-GCNs comprises the steps that collected fMRI images are segmented into a plurality of ROIs through AAL, and BOLD sequence groups of all the ROIs are extracted; carrying out linear normalization processing on a time-frequency graph generated by carrying out wavelet transformation on the BOLD sequence, and estimating FCNs between sample ROIs for obtained two-dimensional sequence data by using Pearson's correlation; dividing the processed data set into a training set, a verification set and a test set; building an ARLSTM-GCNs diagnosis model, wherein the ARLSTM-GCNs diagnosis model comprises an improved AR-LSTM embedded model and an improved GCN-SAGPool model; and based on the constructed ARLSTM-GCNs model, executing a staged training task. According to the method, the autism can be accurately predicted.

The invention discloses an encryption communication method and system based on biological genetic coding chaos driving, and the method comprises the steps: receiving an original bit stream and an initial secret key, and generating a chaos sequence group according to the initial secret key through two hyperchaos models; performing DNA coding encryption on the original bit stream by using the chaos sequence to obtain a gene transmission sequence, and performing DNA decoding on the gene transmission sequence to obtain a transmission bit stream; performing constellation mapping on the transmission bit stream to obtain a constellation diagram, and performing subcarrier scrambling, symbol scrambling and constellation rotation on the constellation diagram according to the chaos sequence; performing OCDM modulation on the constellation diagram through an inverse discrete Fresnel transformation matrix to obtain a transmission signal; hiding the initial key in a frame header of a transmission signal, and sending the transmission signal to a receiver through a transmitter; according to the invention, multi-layer encryption of the OCDM signal and dynamic secret key follow-up transmission are realized.

Provided are compositions and methods for selectively reducing the amount of antibiotic resistant and / or virulent bacteria in a mixed bacteria population, or for reducing any other type of unwanted bacteria in a mixed bacteria population. The compositions and methods involve targeting bacteria that are differentiated from other members of the population by at least one unique clustered regularly interspaced short palindromic repeats (CRISPR) targeted DNA sequence. The compositions and methods can be readily adapted to target any bacteria or any bacteria plasmid, or both.

The present invention addresses the problem of providing: a novel biomarker useful for the prediction of prognosis of a cancer patient or for the detection of cancer; a means capable of specifically recognizing the biomarker; and a novel cancer therapeutic agent.　Provided is a biomarker for cancer, the biomarker comprising claudin 16 (CLDN16) protein or a peptide fragment thereof, or a transcript of CLDN16 gene or a nucleic acid fragment thereof. Also provided is an anti-CLDN16 antibody or a fragment thereof, which comprises: a heavy-chain variable region that includes CDR1 comprising the amino acid sequence represented by SEQ ID NO: 1, CDR2 comprising the amino acid sequence represented by SEQ ID NO: 2, and CDR3 comprising the amino acid sequence represented by SEQ ID NO: 3; and a light-chain variable region that includes CDR1 comprising the amino acid sequence represented by SEQ ID NO: 4, CDR2 comprising the amino acid sequence represented by SEQ ID NO: 5, and CDR3 comprising the amino acid sequence represented by SEQ ID NO: 6.

Provided are a method for constructing a soluble expression plasmid mutant library, and the use thereof. The method for constructing the soluble expression plasmid mutant library comprises: introducing mutations into a ribosome binding site on a soluble expression plasmid, a sequence composition and length of a region between the ribosome binding site and an initiation codon of a fusion protein to be expressed, and a translation initiation region, so as to obtain a mutant library. By means of the method, plasmid mutants with increased expression levels are obtained, thereby increasing the expression level of a target fusion protein and the final yield of a target polypeptide. The present invention solves the problem of low soluble expression levels of certain polypeptides in Escherichia coli.

According to the context-understanding-oriented generative reading understanding method and device and the medium, vector representation of a to-be-read understanding text is obtained by using an encoder of a pre-training language model, a pipeline decoder is used during decoding, an understanding result is generated for the vector representation in a parallel decoding mode, the pipeline decoder is a stacked Transformer decoder, and the pipeline decoder is used for decoding the to-be-read understanding text. And starting to generate a new sub-sequence every delay time delta t, generating the first lexical element of the sub-sequence by depending on the lexical element generated by the previous sub-sequence until the maximum sub-sequence number is reached or all the previous sub-sequences are subjected to lexical element generation, and forming a final understanding result by the sub-sequences obtained by decoding. The pipeline decoder has good performance in reading and understanding of phrase-level and sentence-level data sets, and compared with a sequence decoder, the pipeline decoder has higher reasoning speed and lower GPU (Graphics Processing Unit) memory usage amount, and the generation quality can meet the requirement.

Provided are compositions and methods for selectively reducing the amount of antibiotic resistant and / or virulent bacteria in a mixed bacteria population, or for reducing any other type of unwanted bacteria in a mixed bacteria population. The compositions and methods involve targeting bacteria that are differentiated from other members of the population by at least one unique clustered regularly interspaced short palindromic repeats (CRISPR) targeted DNA sequence. The compositions and methods can be readily adapted to target any bacteria or any bacteria plasmid, or both.

Provided are compositions and methods for selectively reducing the amount of antibiotic resistant and / or virulent bacteria in a mixed bacteria population, or for reducing any other type of unwanted bacteria in a mixed bacteria population. The compositions and methods involve targeting bacteria that are differentiated from other members of the population by at least one unique clustered regularly interspaced short palindromic repeats (CRISPR) targeted DNA sequence. The compositions and methods can be readily adapted to target any bacteria or any bacteria plasmid, or both.

A process for the manufacture of glycosylated p75NTR neurotrophin binding protein (NBP)-Fc fusion protein, comprising: a p75NTR(NBP) portion, having at least 85% sequence identitywith Seq ID No. 2; and an immunoglobulin Fc portion. wherein, the p75NTR(NBP) and Fc portions are connected via a linker, the linker comprises a peptide of formula Gx, where x is 1, 2, 3, 4, 5 or 6 wherein the linker does not comprise or consist of the sequence GGGGS, characterised by expression from CHO cells, wherein cells are harvested within earlier of 30 hours of cell culture viability reaching 75% or, 12 days post inoculation.

Disclosed herein are engineered LSR sequences, compositions, and uses including in genome-editing systems and therapeutic compositions. The LSRs can facilitate nucleic acid recombination at particular attachment sites, using suitable donor sequences. The disclosed LSRs may be used to site-specifically recombine or integrate nucleic acid sequences for a variety of purposes, including treatment of human diseases.

The invention discloses an LAMP (loop-mediated isothermal amplification) detection primer group for flavobacterium psychrophilum and application of the LAMP detection primer group, and belongs to the technical field of molecular biology. The method comprises the following steps: firstly, searching a specific sequence which is completely not matched with other genomes according to a whole genome sequence of flavobacterium psychrophilum, and designing a specific LAMP detection primer group according to the specific sequence; the LAMP detection primer group comprises a pair of outer primers and a pair of inner primers, and the sequence compositions of the outer primers and the inner primers are respectively shown as SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3 and SEQ ID NO: 4. The primer group is used for constant-temperature fluorescent LAMP detection of flavobacterium psychrophilum, and the result shows that the LAMP detection primer group designed by the invention has a good amplification effect on detection of target flavobacterium psychrophilum, and has the advantages of strong specificity, high sensitivity, convenience and rapidness in operation and the like when being used for detecting flavobacterium psychrophilum. The method is suitable for early screening, accurate diagnosis and epidemic situation detection of freshwater fish bacterial diseases caused by flavobacterium psychrophilum.

The invention belongs to the technical field of biological detection, and discloses a sequence combination for rapidly detecting influenza A based on CRISPR / CAS12a-MIRA and application, the sequence combination comprises an MIRA primer pair (SEQ ID NO: 1-2) of a targeted influenza A virus M gene highly conserved region (M1 fragment), and CRISPR / Cas12a detection probes (crRNA SEQ ID NO: 3 and phosphorothioate modified FAM-biotin probe SEQ ID NO: 4). By optimizing a buffer system (containing PEG 8000 and betaine), MIRA amplification and CRISPR detection are continuously completed in a closed tube at 37-42 DEG C, and a result is output within 30 minutes. The detection sensitivity of the kit reaches 50 copies / [mu] L, the specificity is 100%, and subtypes such as H1N1, H3N2, H5N1 and H7N9 can be covered. Compared with the prior art, according to the scheme, the detection time is shortened by 67%, the probe cost is reduced by 80%, and high anti-interference performance (tolerance to 10% mucoprotein and 5% whole blood) is achieved. The kit is suitable for on-site instant detection of saliva, nasopharynx swabs and environmental samples, and an efficient and portable solution is provided for basic medical institutions and prevention and control of sudden epidemic situations.

The invention provides a quality control kit of a nucleic acid detection system as well as a quality control method and application of the quality control kit. The quality control kit comprises the coliphage MS2 and a first sequence group. The first sequence group comprises a forward primer of a sequence as shown in SEQ IQ NO: 1, a reverse primer of a sequence as shown in SEQ IQ NO: 2 and a probe of a sequence as shown in SEQ IQ NO: 3. The quality control method comprises the following steps: mixing a specimen with bacteriophage MS2, and carrying out nucleic acid extraction to obtain an eluent; and mixing an eluent with the feline blood plasma bacterial plastid, the first sequence group and the second sequence group, and carrying out multiple real-time polymerase chain reaction. The quality control kit of the nucleic acid detection system is used for evaluating the recovery rate of nucleic acid and the whole-process quality control of the nucleic acid detection system.

A filter structure is provided. The filtering structure uses a hierarchical network structure comprising one or more network frames for obtaining a high quality sequence. Each network frame includes a base network (450) module and a sequence filter (460). The base network (450) generates one or more sequencing quality indicators. The sequencing quality indicator may represent a quality of accuracy of a base interpretation for each base in the sequence, an individual quality of one or more sequences, or an overall quality of a set of sequences. A sequence filter (460) generates filtering results based on the various filtering policies based on the one or more sequencing quality indicators.

The application provides a nucleic acid sequence and a method for detecting biomolecules based on a self-driven hybrid chain reaction (SHCR), which comprises a priming chain AI, a blocking chain B, a hairpin probe H1, a hairpin probe H2, a hairpin probe H3 and a hairpin probe H4, the priming chain AI comprises an aptamer sequence of a to-be-detected biomolecule and a starting sequence I, the sequence of the starting sequence I is a*‑b*, wherein a* and b* are gene fragments; the 5' end of the hairpin probe H1 is connected with b*, and the 3' end of the hairpin probe H3 is connected with a*. The application further provides a kit for detecting biomolecules based on the SHCR and a method for detecting biomolecules, in particular, a method for detecting a kanamycin residual amount in food. Through ingenious design, when the to-be-detected biomolecule exists, the sequence combination of the above biomolecule can initiate the self-driven hybrid chain reaction, the priming chain can be autonomously supplemented, the self-driven reaction process is reversely activated, thereby a more significant FRET signal is generated, and the detection sensitivity is improved.

The application discloses a sequence construction method and system. The method constructs a switching matrix based on the number of various types of elements in the sequence, establishes a unified constraint system, and obtains a numerical solution of the switching matrix through optimization. Based on the numerical solution, a graph structure is constructed and connectivity is determined to determine whether a complete sequence can be formed; when the connectivity is satisfied, a target sequence that meets the switching relationship and quantity requirements is generated based on the graph structure. The method supports segment-level structure expression, modeling of the head-tail relationship of the augmented matrix, and sub-sequence combination expression, and is suitable for multiple sequence construction tasks. Accordingly, the application also provides a sequence construction system, which includes a matrix modeling module, a constraint construction module, a solving module, a connectivity determination module, and a sequence generation module.

The invention relates to the technical field of nucleic acid detection, and particularly provides a CRISPR-Cas12a cascade signal amplification system and application thereof. The system comprises a first-stage sensing unit, a second-stage sensing unit and a third-stage sensing unit, wherein the first-stage sensing unit is configured to recognize a target to be detected and activate the trans-cleavage activity of first-stage Cas12a protein; an engineered scaffold RNA composed of an RNA sequence and a DNA sequence is cut, the obtained RNA sequence serves as an activator of a second-stage Cas12a protein, and the second-stage Cas12a protein obtains trans-cleavage activity, so that a fluorescence report probe is cut, and a signal is output. According to the system, an activation event of a first Cas12a is converted into an exponential signal output unit of a second Cas12a, and a cascade amplification network is formed. According to the design, background signal leakage is eradicated from a physical mechanism, and the detection sensitivity is remarkably improved. The detection method provided by the invention is low in cost, high in sensitivity and high in reaction speed.

The application provides a data expansion method and device, the method comprises the following steps: obtaining N groups of real source data, each group of real source data contains a data sequence; dividing the data sequences in the N groups of real source data into a plurality of sequence combinations, each sequence combination is a calculation group; performing weighted calculation on each calculation group according to the sequence number requirement of weighted average calculation, to obtain the weight value allocated to each calculation group; performing weighted average on the data sequences in each calculation group according to the weight value allocated to each calculation group, to obtain the data expansion sequence of each calculation group. In the data expansion method, the calculation results of all calculation groups are not all considered all data sequences, therefore, in the case that some part of data sequences are abnormal, more calculation results are not affected because they do not involve abnormal data sequences, which greatly avoids the influence of abnormal sequences in small data on the final data expansion sequence.

Split-pegRNA recorder reagents and methods are provided that provide specific molecular interactions within cells induce precise genome editing that inserts an event-specific barcode sequence into the predetermined genomic locus of DNA Tape. The split pegRNAs include (a) a crRNA component, including in 5′ to 3′ order (i) a spacer sequence for a genomic locus of interest; (ii) a crRNA repeat sequence necessary for binding to a CRISPR-Cas protein; and (iii) a first RNA extension; and (b) a petracrRNA component, including in 5′ to 3′ order (i) a second RNA extension; (ii) a petracrRNA antirepeat sequence necessary for binding to a CRISPR-Cas protein; (iii) a gRNA scaffold; wherein the crRNA component and the petracrRNA component are not covalently bound to each other.