112 results about "Sequenceome" patented technology

The invention discloses a CRISPR / Cas12a-RPA (clustered regularly interspaced short palindromic repeats / CRISPR associated protein 12a-recombinase polymerase amplification)-based sequence combination for rapidly detecting mouse hepatitis virus and a kit thereof, and belongs to the technical field of biological detection The sequence combination comprises an RPA primer pair sequence, a crRNA sequence and an ssDNA probe sequence; the kit comprises an RPA primer pair sequence, a crRNA sequence and an ssDNA probe sequence, the kit further comprises a mouse hepatitis virus negative control standard substance, RNase-free water, an RPA reaction buffer solution, an NEB buffer solution, Lba Cas12a nuclease, dry powder RPA reaction microspheres containing recombinase polymerase and magnesium acetate with the concentration of 280 mM. The kit can be used for rapidly detecting the mouse hepatitis virus. The primer sequence combination provided by the invention is good in conservative property and specificity and relatively high in sensitivity, and rapid detection of the mouse hepatitis virus can be realized.

The invention provides a method for eliminating ribosomal RNA in a mouse total RNA sample, which comprises the following steps: carrying out reverse transcription on the total RNA in the mouse sample into cDNA, and amplifying the cDNA by adopting a primer sequence group to obtain a DNA fragment covering all sequences of the mouse ribosomal RNA; carrying out amplification by taking the DNA fragment as a template and adopting a single primer in the primer sequence group to obtain a single-stranded DNA probe; hybridizing the total RNA of the mouse sample by adopting a single-stranded DNA probe, and performing RNase H digestion to obtain a digestion product; sequentially carrying out DNase I digestion and RNA purification on the digestion product to obtain a target product; wherein the primer sequence group comprises upstream primers from the first to the nineteenth and downstream primers from the first to the nineteenth, and the primer sequence group has the characteristics of low cost, stable performance and high reduction efficiency.

The invention belongs to the field of canine distemper virus detection, and particularly relates to a canine distemper virus RT-RAA CRISPR / Cas 12b detection kit and a detection method thereof. According to the kit, isothermal amplification is performed by using an RT-RAA universal primer, and then detection is performed by using a CRISPR / Cas 12b detection system consisting of target sequence sgRNA, so that amplification of a target fragment of the N gene of the canine distemper virus and target detection visualization can be effectively realized. The result of the embodiment shows that the RT-RAA CRISPR / Cas 12b detection kit finally designed by the invention is high in specificity and does not have cross reaction with other viruses, the lowest visual detection limit reaches 4.77 * 10 < 1 > copies / mu L, the virus can be instantly detected when the RT-RAA CRISPR / Cas 12b detection kit is used for detecting the CDV, the labor and equipment cost is low, the period is short, and the detection time does not exceed 2 hours. The rapid detection technology can be popularized and applied to epidemiological investigation and epidemic situation monitoring of the canine distemper virus, is suitable for epidemic disease monitoring of wild animals, quarantine of zoos and on-site rapid detection of clinical samples of canines, and has good practical significance and wide market prospects.

Provided are a method for constructing a soluble expression plasmid mutant library, and the use thereof. The method for constructing the soluble expression plasmid mutant library comprises: introducing mutations into a ribosome binding site on a soluble expression plasmid, a sequence composition and length of a region between the ribosome binding site and an initiation codon of a fusion protein to be expressed, and a translation initiation region, so as to obtain a mutant library. By means of the method, plasmid mutants with increased expression levels are obtained, thereby increasing the expression level of a target fusion protein and the final yield of a target polypeptide. The present invention solves the problem of low soluble expression levels of certain polypeptides in Escherichia coli.

Disclosed herein are engineered LSR sequences, compositions, and uses including in genome-editing systems and therapeutic compositions. The LSRs can facilitate nucleic acid recombination at particular attachment sites, using suitable donor sequences. The disclosed LSRs may be used to site-specifically recombine or integrate nucleic acid sequences for a variety of purposes, including treatment of human diseases.

The application provides a nucleic acid sequence and a method for detecting biomolecules based on a self-driven hybrid chain reaction (SHCR), which comprises a priming chain AI, a blocking chain B, a hairpin probe H1, a hairpin probe H2, a hairpin probe H3 and a hairpin probe H4, the priming chain AI comprises an aptamer sequence of a to-be-detected biomolecule and a starting sequence I, the sequence of the starting sequence I is a*‑b*, wherein a* and b* are gene fragments; the 5' end of the hairpin probe H1 is connected with b*, and the 3' end of the hairpin probe H3 is connected with a*. The application further provides a kit for detecting biomolecules based on the SHCR and a method for detecting biomolecules, in particular, a method for detecting a kanamycin residual amount in food. Through ingenious design, when the to-be-detected biomolecule exists, the sequence combination of the above biomolecule can initiate the self-driven hybrid chain reaction, the priming chain can be autonomously supplemented, the self-driven reaction process is reversely activated, thereby a more significant FRET signal is generated, and the detection sensitivity is improved.

The application discloses a sequence construction method and system. The method constructs a switching matrix based on the number of various types of elements in the sequence, establishes a unified constraint system, and obtains a numerical solution of the switching matrix through optimization. Based on the numerical solution, a graph structure is constructed and connectivity is determined to determine whether a complete sequence can be formed; when the connectivity is satisfied, a target sequence that meets the switching relationship and quantity requirements is generated based on the graph structure. The method supports segment-level structure expression, modeling of the head-tail relationship of the augmented matrix, and sub-sequence combination expression, and is suitable for multiple sequence construction tasks. Accordingly, the application also provides a sequence construction system, which includes a matrix modeling module, a constraint construction module, a solving module, a connectivity determination module, and a sequence generation module.

The invention relates to the technical field of nucleic acid detection, and particularly provides a CRISPR-Cas12a cascade signal amplification system and application thereof. The system comprises a first-stage sensing unit, a second-stage sensing unit and a third-stage sensing unit, wherein the first-stage sensing unit is configured to recognize a target to be detected and activate the trans-cleavage activity of first-stage Cas12a protein; an engineered scaffold RNA composed of an RNA sequence and a DNA sequence is cut, the obtained RNA sequence serves as an activator of a second-stage Cas12a protein, and the second-stage Cas12a protein obtains trans-cleavage activity, so that a fluorescence report probe is cut, and a signal is output. According to the system, an activation event of a first Cas12a is converted into an exponential signal output unit of a second Cas12a, and a cascade amplification network is formed. According to the design, background signal leakage is eradicated from a physical mechanism, and the detection sensitivity is remarkably improved. The detection method provided by the invention is low in cost, high in sensitivity and high in reaction speed.

The application provides a data expansion method and device, the method comprises the following steps: obtaining N groups of real source data, each group of real source data contains a data sequence; dividing the data sequences in the N groups of real source data into a plurality of sequence combinations, each sequence combination is a calculation group; performing weighted calculation on each calculation group according to the sequence number requirement of weighted average calculation, to obtain the weight value allocated to each calculation group; performing weighted average on the data sequences in each calculation group according to the weight value allocated to each calculation group, to obtain the data expansion sequence of each calculation group. In the data expansion method, the calculation results of all calculation groups are not all considered all data sequences, therefore, in the case that some part of data sequences are abnormal, more calculation results are not affected because they do not involve abnormal data sequences, which greatly avoids the influence of abnormal sequences in small data on the final data expansion sequence.

Split-pegRNA recorder reagents and methods are provided that provide specific molecular interactions within cells induce precise genome editing that inserts an event-specific barcode sequence into the predetermined genomic locus of DNA Tape. The split pegRNAs include (a) a crRNA component, including in 5′ to 3′ order (i) a spacer sequence for a genomic locus of interest; (ii) a crRNA repeat sequence necessary for binding to a CRISPR-Cas protein; and (iii) a first RNA extension; and (b) a petracrRNA component, including in 5′ to 3′ order (i) a second RNA extension; (ii) a petracrRNA antirepeat sequence necessary for binding to a CRISPR-Cas protein; (iii) a gRNA scaffold; wherein the crRNA component and the petracrRNA component are not covalently bound to each other.

Embodiments of the present disclosure provide a data processing method and apparatus, a storage medium and an electronic apparatus. The method includes: dividing data to be transmitted into G groups, wherein each group respectively includes D(g) subgroups, each of the subgroups includes one or more pieces of data, g is an identifier corresponding to each group, g=1, 2, . . . , G, and D(g)>=1; respectively performing first processing on the D(g) subgroups in each group to obtain D(g) groups of first data sequences; performing second processing on the D(g) groups of first data sequences in each group to obtain a second data sequence of each group; and transmitting G groups of the second data sequences. By means of some embodiments of the present disclosure, the problem of transmission interference in the related art can be solved.

Various aspects of the present disclosure generally relate to wireless communication. In some aspects, a user equipment (UE) may receive a probabilistic shaping configuration including a configuration for peeling-based arithmetic coding. The UE may transmit an uplink communication in accordance with the probabilistic shaping configuration, wherein the configuration for peeling-based arithmetic coding includes a configuration for determining a sequence composition during a composition element selection stage and a configuration for generating a sequence during a sequence generation stage, wherein the configuration for generating the sequence during the sequence generation stage includes a configuration for generating the sequence in accordance with the sequence composition determined during the composition element selection stage. Numerous other aspects are described.

The present invention refers to a sgRNA molecule comprising a targeting domain for specifically targeting a SNP in the BEST1 coding region of a pathologic allele, wherein said targeting domain consists of a sequence selected from the group consisting of SEQ ID NO: 3-8, 41-44, 14-20, 50-52 and 54-55, or a sgRNA molecule combination of specifically defined first and second sgRNA molecules, wherein the first and the second sgRNA molecule each comprise a targeting domain for specifically targeting a SNP in the BEST1 gene coding or non-coding region of a pathologic allele. The present invention also refers to a nucleic acid comprising a sequence that encodes the sgRNA molecule or sgRNA molecule combination and to nucleic acid combinations. The present invention further relates to a recombinant adenovirus-associated virus (AAV) comprising the nucleic acids according to the present invention or recombinant AAV combinations. The sgRNA molecule, the nucleic acid, the recombinant AAV and combinations are useful tools for editing of the target domain in the bestrophin-1 (BEST1) gene to restore BEST1 channel function by e.g. CRISPR / Cas9)-based gene editing. The present invention further relates to the sgRNA molecule, the sgRNA molecule combination, the nucleic acid, the nucleic acid combination, the recombinant AAV and the recombinant AAV combination for use in a method for treatment of the human or animal body by surgery or therapy and for use in method of treating or preventing BEST1-related retinopathies, in particular autosomal dominant BEST1-related retinopathies.

This application provides a data transmission method and an apparatus. In an example method, a first sequence and a common sequence group are obtained, where a quantity of sequences in the common sequence group is N; the first sequence includes K first sub-sequences, lengths of the K first sub-sequences are sequentially B1, B2, . . . , BK, K is a positive integer, B1=log2N, and the length B1 of a 1st first sub-sequence is greater than or equal to a length of another first sub-sequence. The K first sub-sequences are separately mapped into K second sub-sequences based on the common sequence group. A second sequence including the K second sub-sequences is output.

The application discloses a test strip RPA primer for detecting potato brown stem nematode, wherein the primer is composed of a nucleotide sequence shown in SEQ ID No:1 and SEQ ID No:2; a combination of the primer and a probe is also disclosed, wherein the probe is composed of a sequence shown in SEQ ID No:3. In addition, a detection kit comprising the primer and the probe combination is disclosed. The test strip RPA primer and the probe combination of the application have the advantages of high specificity and sensitivity for detecting potato brown stem nematode, low requirement for equipment and personnel quality, simple operation, fast detection speed and low cost, and are suitable for port quarantine and primary site detection.

Methods, kits, and oligonucleotides used in the detection of coronavirus, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), are disclosed. In some aspects, the oligonucleotides are primers or probes used in the described methods or kits. The nucleotide sequence of the oligonucleotide consists of 300 or less, 150 or less, or 40 or less continuous nucleotides from a nucleotide sequence selected from the group consisting of: SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, and SEQ ID NO: 5, or is a variant thereof. In some embodiments, the oligonucleotide is modified with an internal spacer or a detectable label. For example, the 5′ terminus is labeled with a fluorophore and the 3′ terminus is complexed to a quencher of fluorescence of said fluorophore. In some embodiments, the nucleotide sequence of the oligonucleotide further comprises a universal tail sequence.

The invention discloses a kit for rapidly and quantitatively detecting an integrated HIV (Human Immunodeficiency Virus) previrus genome and application of the kit. The kit comprises an anchor primer in an Alu region in an HIV virus, and degenerate primers in gag, nef and LTR regions of an HIV sequence. According to the kit disclosed by the invention, after pre-amplification, the integrated copy number AU-LTR detected by the QPCR is obviously improved compared with AU-gag and AU-nef, and the capture efficiency is higher. Meanwhile, the kit provided by the invention can be used for carrying out three-generation sequencing on a detected sample with a relatively high integration level on the basis of Alu-LTR capture and detection of an integrated copy number, and can be used for carrying out sequence composition and genome position analysis on a storage library of HIV (Human Immunodeficiency Virus).

This invention relates to the field of biotechnology, specifically disclosing a Decacovirus nucleic acid detection composition based on RT-RPA and CRISPR / Cas13a technologies and its applications. This invention successfully obtains a novel method for Decacovirus nucleic acid detection based on RT-RPA and CRISPR / Cas13a technologies. The nucleic acid detection composition includes: an RT-RPA primer pair and crRNA; the RT-RPA primer pair consists of two RT-RPA primers, with sequences shown in SEQ ID NO.1 and SEQ ID NO.2; the crRNA sequence consists of an anchoring sequence for binding to the Cas13a protein and a guide sequence targeting the Decacovirus gene target sequence, with the guide sequence shown in positions 23-50 of SEQ ID NO.3. Using this composition for Decacovirus detection offers advantages such as high specificity, high sensitivity, rapid and convenient operation, solving the problem of inconvenience in current field monitoring of the virus's prevalence.

The invention relates to the technical field of agriculture, in particular to an efficient ABE base editing tool based on Cas12i3 and application of the efficient ABE base editing tool. The Cas12i3-based efficient ABE base editing tool provided by the invention comprises a protein expression cassette and an RNA (Ribonucleic Acid) expression cassette, the protein expression cassette comprises a promoter, a 2 * BP NLS nuclear localization signal fragment, a 2 * adenine deaminase TadA8e, a connecting peptide, a Cas12i3 notch enzyme fragment and a terminator, the RNA expression cassette comprises a promoter, a crRNA sequence and a terminator; wherein the crRNA sequence is composed of a DR sequence and a target specific spacer sequence. The tool realizes efficient and specific single base substitution of A-to-G in a rice genome by utilizing the targeted binding characteristic of Cas12i3 and the efficient deamination activity of 2 * TadA8e, has the advantages of high editing efficiency, low off-target rate, wide application range and the like, provides an efficient and accurate technical means for plant functional genome research and molecular breeding, and has a wide application prospect. The creation of excellent rice varieties can be accelerated.

The application provides a blood stream infection related septic shock multi-modal fusion prediction method and system, belonging to the field of intelligent medical treatment, and solving the problems of long time consumption, insufficient sensitivity and insufficient multi-modal information analysis capability in blood stream infection related septic shock. The method comprises: acquiring multi-modal clinical data of a target patient suspected of blood stream infection within a preset observation time window to obtain a multi-modal multi-scale feature sequence formed by combining each modality spatiotemporal feature vector sequence; inputting the multi-modal multi-scale feature sequence into a time sequence encoding network based on a multi-head attention mechanism to obtain an aligned multi-modal time sequence representation sequence; setting a learnable modality weight parameter for each modality, and updating the learnable modality weight parameter, the encoder and the time sequence encoding network through a task loss function according to the label of whether blood stream infection related septic shock occurs or not in the training process to obtain a multi-modal fusion model for prediction.

A siRNA characterised by having a sequence selected from the group comprised of: - SEQ ID NO. 1 : 5 ' CUCUUUCCCUUGAAUAAGC 3 '; - SEQ ID NO. 2 : 5 ' UGAUUCCCAAAGCUCACAG 3 '; and - a sequence including at least 16 contiguous nucleotides differing by no more than 3 nucleotides from SEQ ID No. 1 or SEQ ID No. 2, to silence TRAPl-low SEQ ID N°3. The use of the 5 'UTR SEQ ID N°4 region of the TRAPl-low transcript SEQ ID N°3 or a portion of that 5 'UTR region selected from the group comprised of: - the SEQ ID No. 6 portion of said 5 ' UTR region comprising nucleotide positions 1 through 644; - a portion of said region 5 ' UTR comprising the portion of SEQ ID No. 6 extended by a predetermined number of nucleotide positions beyond nucleotide position 644 along said region 5 'UTR.

The present application relates to the technical field of microorganism detection, and in particular to a sequence combination for detecting total amount of bacteria based on a CRISPR / Cas12a system and application, the sequence combination comprising at least one of crRNAs shown in SEQ ID NO: 1-3.The present application also provides a corresponding detection method, which realizes the detection of total amount of bacteria based on the CRISPR / Cas12a system for the first time, and provides a good technical means for the quantitative detection of total amount of bacteria.The detection method based on the CRISPR / Cas12a system provided by the present application is completed under isothermal conditions, does not require professional instruments and equipment, can be quickly and simply detected, and has good sensitivity.

The present disclosure provides isolated monoclonal anti-CD3epsilon antibodies or antigen-binding fragments thereof comprising one or more heavy chain CDR sequences selected from the group consisting of: SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, and 47, and / or one or more kappa light chain CDR sequences selected from the group consisting of: SEQ ID NOs: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46 and 48, isolated polynucleotides encoding the same, pharmaceutical compositions comprising the same, and the use thereof.

The invention relates to an HER2 protein detection sequence group based on a tetrahedral nucleic acid framework and catalytic hairpin assembly, which comprises tetrahedral skeleton sequences S1, S2, S3 and S4, a cholesterol modified sequence Chol, an aptamer chain sequence L and hairpin probes H1, H2 and H3, and the 5'end of the tetrahedral skeleton sequence S1 extends to form an HER2 aptamer sequence HApt; the aptamer chain sequence L and the HER2 aptamer sequence HApt part are complementarily paired; the 3'ends of the tetrahedral skeleton sequences S2, S3 and S4 extend to form connecting sequences used for being combined with a cholesterol modification sequence Chol. By adopting the technical scheme, the invention provides the HER2 protein detection sequence group based on the tetrahedral nucleic acid framework and catalytic hairpin assembly, the kit and application thereof, and an HER2 in-situ detection technology with high stability, excellent membrane anchoring capability and signal amplification function.

The invention relates to the technical field of processing and application of bioactive peptides, and particularly discloses a preparation method of suaeda salsa oligopeptide and a determination method of sequence composition of the suaeda salsa oligopeptide, the molecular weight of the suaeda salsa oligopeptide is less than 2KDa, and the suaeda salsa oligopeptide contains 48 peptide fragment sequences and has antioxidant activity and in-vitro lipid lowering effect; crude protein is extracted from suaeda salsa and separated to obtain suaeda salsa oligopeptide, the sequence composition of the suaeda salsa oligopeptide is analyzed and determined, and the antioxidant activity and the in-vitro lipid-lowering effect of the suaeda salsa oligopeptide are detected. Compared with the suaeda salsa crude protein, the oligopeptide has good biological activity, is easier to absorb and utilize, and can be widely applied to the industries of medicines, special medical foods, health care products and the like.

The present disclosure provides isolated monoclonal anti-CD3epsilon antibodies or antigen-binding fragments thereof comprising one or more heavy chain CDR sequences selected from the group consisting of: SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, and 47, and / or one or more kappa light chain CDR sequences selected from the group consisting of: SEQ ID NOs: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46 and 48, isolated polynucleotides encoding the same, pharmaceutical compositions comprising the same, and the use thereof.

A method for producing a SARS-CoV-2 virus-like particle-based protein subunit vaccine. The method comprises creating a fusion protein by combining a DNA sequence encoding an iLOV protein with a DNA sequence encoding a peptide linker and a cleavage site for enterokinase protease and a DNA sequence encoding a Receptor Binding Domain (RBD) of the SARS-CoV-2 viral spike protein. The RBD protein is attached to a “Spy Tag” peptide. The peptide linker cleavage site DNA sequence is between the iLOV protein DNA sequence and either the SARS-CoV-2 viral protein DNA sequence or the “Spy Tag” peptide DNA sequence. The DNA sequence encoding the fusion protein is introduced into a P. pastoris host to form transformants. At least one optimal recombinant is identified from the transformants using fluorescence to detect optimal expression levels of the SARS-CoV-2 viral protein. The SARS-CoV-2 viral protein is isolated from the fusion protein by cleaving the iLOV protein and linker sequences from the target protein. The RBD sequence can be mutated to represent RBD variants or homologous sequences or improve expression and alter its glycosylation pattern. The RBD sequence can belong to any virus within the coronavirus family.

Embodiments of the present application disclose a person care method and device, computer equipment and a storage medium. The method comprises: obtaining log data of each door magnetic sensor arranged at a residence of a monitored person to obtain original sequence group data; performing adaptive fuzzy clustering on the original sequence group data to obtain a plurality of sequence group sets; determining a clustering center of each sequence group set and a maximum distance of the clustering center, and setting the maximum distance of the clustering center as a time deviation threshold; obtaining a monitoring signal collected by each door magnetic sensor in real time; determining whether a distance between current monitoring data and any sequence group set is within the corresponding time deviation threshold; if yes, determining that the current monitoring data are matched with the plurality of sequence group sets successfully; determining a behavior category of the monitored person according to the matched sequence group set; and if not, generating a remote reminding information. The method of the embodiments of the present application can improve the safety care efficiency of the monitored person.

The invention discloses a sequence combination, a kit and a method for co-detecting a new bunyavirus and an anaplasma phagocytophilum based on RPA-CRISPR, and application of the sequence combination, the kit and the method. Comprising a first RPA primer pair and a first crRNA which are designed according to an SFTSV L segment conserved region, a second RPA primer pair and a second crRNA which are designed according to an AP 16S rRNA gene conserved region, an optimized universal T7 auxiliary primer, a kit prepared by using the sequence combination, and a detection method for detecting the new bunyavirus and the anaplasma phagocytophilum by using the kit. According to the detection method disclosed by the invention, an RPA amplification method and a CRISPR detection method are combined to detect the new bunyavirus and the anaplasma phagocytophilum at the same time, and the bottlenecks in co-amplification efficiency and orthogonal detection sensitivity in the prior art can be effectively overcome.