The invention discloses a method for realizing multi-site sequence
molecular typing on
haemophilus parasuis. According to the method, the operation technology of carrying out
molecular typing on the
haemophilus parasuis by adopting the multi-site sequence
typing (MLST) method is disclosed for the first time. In the method, the
genome DNA of the
pathogenic bacteria is extracted, 7 house-keeping genes including
rpoB, atpD, g3pd, infB, mdh, _6pgd and frdB are adopted for carrying out screening, amplification, detection and sequencing, CExpress and DNAMAN
software is adopted for carrying out splicing, multi-
sequence alignment and analysis on the sequencing result, and then the sequence type (ST) of the strain is obtained through an on-line
haemophilus parasuis MLST
typing database. The amplification primers used by the
typing technology are adopted for the first time, the PCR
reaction system and conditions automatically optimized, and the technique process can be used for the classification identification and
genetic diversity research of haemophilus parasuis.