The application discloses an application method of a
sequence search tool CircBLAST considering
gene sequence evolution rearrangement, and belongs to the technical field of
bioinformatics. The method flow comprises the following steps: firstly, all
protein sequences are
cut according to the length of a required word_size, a
data set is constructed in combination with
sequence annotation data, and is written into a
database; then, a request sequence is prepared, and is
cut into small fragments of word_size; further, a search matching, construction of a circular sequence and
sequence alignment are carried out to complete a retrieval process; finally, an alignment result containing matching fragments, a similarity
score and the like information is generated, is used for presenting to a user for viewing and judging the reliability of matching. The application considers the evolution rearrangement of
gene sequences, significantly improves the accuracy of
sequence alignment, and can find more reordered sequences.